Strain Gene Zyg FClass FTerms similarity score p-value rank description GOBP GOMF GOCC YGR154C GTO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 1 0 1 Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization glutathione metabolic process glutathione transferase activity peroxisome YGL102C_d YGL102C het 0.16275724445875 2.34364766819705e-21 2 Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Unknown Unknown Unknown YDL199C_p YDL199C hom 0.114140094622575 3.39113427148324e-11 3 Putative transporter, member of the sugar porter family transport|transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YNL256W FOL1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.108773241329653 2.65403796247814e-10 4 Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities tetrahydrofolate biosynthetic process dihydroneopterin aldolase activity|2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity|dihydropteroate synthase activity mitochondrial envelope|mitochondrion|cytoplasm YOL079W_d YOL079W hom 0.105500103258584 8.98035109736633e-10 5 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR480W DIG2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.101052304917383 4.44011883771327e-09 6 MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown transcription factor binding nucleus YLR289W GUF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION|RIBOSOME 0.101017369027614 4.49501950896457e-09 7 Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor positive regulation of translation ribosome binding|GTPase activity mitochondrion|mitochondrial ribosome|mitochondrial matrix YKR009C FOX2 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0975389899204127 1.50418076385282e-08 8 Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities fatty acid beta-oxidation enoyl-CoA hydratase activity|3-hydroxyacyl-CoA dehydrogenase activity peroxisome YLL048C YBT1 hom FT NUCLEOTIDE METABOLISM 0.0965886940761061 2.07449787378351e-08 9 Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters bile acid and bile salt transport bile acid-exporting ATPase activity integral to membrane|fungal-type vacuole YOL158C ENB1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0952179981400461 3.26536094868529e-08 10 Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment ferric-enterobactin transport ferric-enterobactin transmembrane transporter activity integral to membrane|cellular bud membrane|plasma membrane YGR127W_p YGR127W hom 0.0928325459706871 7.14385054715548e-08 11 Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component YLR097C HRT3 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.0909097497108313 1.32425873242172e-07 12 Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YGR211W ZPR1 het FT CELL CYCLE|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0881454849223331 3.21143752717954e-07 13 Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress regulation of mitotic cell cycle|cellular response to starvation protein binding nucleus|cytoplasm YLL063C AYT1 hom 0.0870036886693717 4.46677391039382e-07 14 Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis secondary metabolic process trichothecene 3-O-acetyltransferase activity cellular_component YOR074C CDC21 het FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0858602712617191 6.31525150627556e-07 15 Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S dTMP biosynthetic process thymidylate synthase activity nucleus YKL017C HCS1 hom FT NUCLEUS|CHROMOSOME 0.0853963288737088 7.25899843753907e-07 16 Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities lagging strand elongation ATP-dependent 5'-3' DNA helicase activity DNA helicase A complex|alpha DNA polymerase:primase complex YBL083C_d YBL083C hom 0.0851332458648581 7.85304599105022e-07 17 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Unknown Unknown Unknown YGR131W FHN1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0844870124787832 9.51762962993555e-07 18 Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain establishment of protein localization to plasma membrane molecular_function integral to membrane|membrane raft YLR189C ATG26 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM 0.0804173841684025 3.09399675999501e-06 19 UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy sterol metabolic process|ascospore-type prospore membrane assembly sterol 3-beta-glucosyltransferase activity cytoplasm YAL067C SEO1 hom 0.0795884086789003 3.90751471658525e-06 20 Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide transport transporter activity integral to membrane|membrane YOR062C_p YOR062C hom FT NUCLEUS 0.0795429172838924 3.95763376150333e-06 21 Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YOR329C SCD5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0787477080490535 4.93982485504607e-06 22 Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency protein secretion|endocytosis|actin filament organization|cortical actin cytoskeleton organization protein phosphatase 1 binding nucleus|actin cortical patch YDL124W YDL124W hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0777268857379721 6.54603502008961e-06 23 NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|cellular ketone metabolic process|cellular amide metabolic process|cellular aromatic compound metabolic process alpha-keto amide reductase activity|aldo-keto reductase (NADP) activity|alditol:NADP+ 1-oxidoreductase activity|alpha-keto ester reductase activity nucleus|plasma membrane|cytoplasm YHR032W ERC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0776992222882048 6.59585277407526e-06 24 Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine S-adenosylmethionine biosynthetic process|transmembrane transport antiporter activity integral to membrane YDR061W_p YDR061W hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0766764950109487 8.71434121045826e-06 25 Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion YPL250C ICY2 hom 0.0763493362781531 9.51951263937456e-06 26 Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate mitochondrion degradation molecular_function cellular_component YNL182C IPI3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0740792733841064 1.74072043961488e-05 27 Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene ribosomal large subunit assembly|rRNA processing molecular_function nucleoplasm|nucleus YKL147C_d YKL147C hom 0.0739310070404155 1.80964716276639e-05 28 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3 Unknown Unknown Unknown YHR042W NCP1 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.073907084143544 1.82100921791653e-05 29 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p ergosterol biosynthetic process electron carrier activity mitochondrial outer membrane|mitochondrion YML023C NSE5 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0734722837146086 2.04554582197319e-05 30 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair molecular_function Smc5-Smc6 complex|nucleus YMR103C_d YMR103C hom 0.0733850817495119 2.08651938403919e-05 31 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR041C URB2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0725617508666311 2.61755306147362e-05 32 Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis rRNA metabolic process|ribosome biogenesis molecular_function nucleolus YKR041W YKR041W hom FT NUCLEUS|CYTOSKELETON 0.0720664245048689 2.93908876324757e-05 33 Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking biological_process molecular_function nucleus|mitotic spindle|cytoplasm YOR291W YPK9 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS 0.0714349173230606 3.47949466879968e-05 34 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome biological_process molecular_function fungal-type vacuole membrane|integral to membrane YER056C FCY2 hom FT PLASMA MEMBRANE 0.0711253366378447 3.72268038245696e-05 35 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress cytosine transport|cytidine transport|purine nucleobase transport|transmembrane transport nucleobase transmembrane transporter activity|cytidine transmembrane transporter activity integral to membrane|plasma membrane YBR201W DER1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.070898197635227 3.94211162227578e-05 36 Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p ER-associated protein catabolic process molecular_function integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YKR016W FCJ1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0708110271304767 4.02953820273836e-05 37 Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane cristae formation|protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial crista|mitochondrial crista junction|integral to mitochondrial inner membrane YPL248C GAL4 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0701325040171019 4.77573384678618e-05 38 DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p galactose metabolic process|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus YDR291W HRQ1 hom FT NUCLEUS 0.0694863750737986 5.93077125079303e-05 39 3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) biological_process molecular_function nucleus YFL049W SWP82 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0690880666628796 6.2007318188678e-05 40 Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YDL236W PHO13 hom FT NUCLEUS 0.0687798123092894 6.67109683273612e-05 41 Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity protein dephosphorylation 4-nitrophenylphosphatase activity|phosphoprotein phosphatase activity|alkaline phosphatase activity nucleus|cytoplasm YMR037C MSN2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0684190978601135 7.28567519468257e-05 42 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|cellular response to methylmercury|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YNL050C_p YNL050C hom 0.0664865816745637 0.000115983392756175 43 Putative protein of unknown function; YNL050c is not an essential gene biological_process molecular_function cellular_component YLL056C_p YLL056C hom 0.0663594472784333 0.000120096036578946 44 Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component YDL218W_p YDL218W hom 0.0658181023833544 0.00013583849813139 45 Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin biological_process molecular_function integral to membrane|cytoplasm YDR026C NSI1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0650522524297937 0.000162875042429935 46 RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication termination of RNA polymerase I transcription|chromatin silencing at rDNA|Unknown DNA binding ribosome|nucleolus|rDNA heterochromatin YBR199W KTR4 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0649303408257616 0.000167183346650956 47 Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YDR221W GTB1 hom FT CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM 0.0648517500246785 0.000171412029328614 48 Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress polysaccharide biosynthetic process|protein N-linked glycosylation alpha-glucosidase activity glucosidase II complex|endoplasmic reticulum|endoplasmic reticulum lumen YGL154C LYS5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0645891429383665 0.00018094843698584 49 Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine lysine biosynthetic process via aminoadipic acid|protein-cofactor linkage holo-[acyl-carrier-protein] synthase activity cytoplasm YJL138C TIF2 hom FT TRANSLATION|RIBOSOME 0.0644984874075169 0.000184780991260105 50 Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication translational initiation|regulation of translational initiation ATP-dependent RNA helicase activity|translation initiation factor activity ribosome|eukaryotic translation initiation factor 4F complex|plasma membrane|cytoplasm YNL326C PFA3 hom FT MEMBRANE ORGANIZATION 0.064293989658837 0.00019370823238004 51 Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions vacuole fusion, non-autophagic|protein palmitoylation palmitoyltransferase activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YIL136W OM45 hom FT MITOCHONDRION 0.0641958635676067 0.000198134266498823 52 Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YMR272W-B_p YMR272W-B hom 0.0632876486818212 0.000243855169162875 53 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YCR007C_p YCR007C hom 0.062926559329036 0.000277258292646925 54 Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component YML054C-A_p YML054C-A hom 0.0622904590338095 0.000305359453202064 55 Putative protein of unknown function biological_process molecular_function cellular_component YOR164C GET4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0611661171189869 0.000391997303616786 56 Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 protein insertion into ER membrane|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YLR223C IFH1 het FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0601658145867162 0.000487875635472402 57 Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|chromatin silencing at telomere transcription coactivator activity nuclear chromatin|nucleolus|nucleus|CURI complex YDR196C CAB5 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0600210530922535 0.000503437885578214 58 Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies coenzyme A biosynthetic process dephospho-CoA kinase activity nuclear envelope|mitochondrion|endoplasmic reticulum YMR149W SWP1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0595454294620575 0.000557886123986227 59 Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YBL008W HIR1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0593619588181927 0.000585844856520556 60 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores DNA replication-independent nucleosome assembly|regulation of transcription from RNA polymerase II promoter|transcription elongation from RNA polymerase II promoter nucleosome binding|transcription corepressor activity|DNA binding chromosome, centromeric region|HIR complex|nucleus YBR183W YPC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0593585135100752 0.000580746914488646 61 Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication ceramide catabolic process|ceramide biosynthetic process phytoceramidase activity|sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YIL115C NUP159 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0589259851706745 0.00063703143904298 62 FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|protein export from nucleus|ribosomal large subunit export from nucleus|ncRNA export from nucleus|nuclear pore distribution|ribosomal small subunit export from nucleus nucleocytoplasmic transporter activity|adenyl-nucleotide exchange factor activity nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YBR288C APM3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0587761024111699 0.000657690739394502 63 Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YER091C MET6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0585677171597428 0.000691272613917659 64 Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs methionine biosynthetic process 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity plasma membrane|cytoplasm YDR476C_p YDR476C hom FT ENDOPLASMIC RETICULUM 0.0582100074387626 0.000745543718944683 65 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene biological_process molecular_function endoplasmic reticulum YNL219C ALG9 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0581370630469867 0.000752956130187799 66 Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation mannosyltransferase activity integral to membrane|endoplasmic reticulum YDR022C ATG31 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0574505000095642 0.000869474503716805 67 Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion autophagy|mitochondrion degradation|piecemeal microautophagy of nucleus molecular_function pre-autophagosomal structure|cytoplasm YDL091C UBX3 hom 0.0574139929433977 0.000876115320735929 68 Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10 biological_process molecular_function cytoplasm YKR012C_d YKR012C hom 0.0572243324036887 0.000911378319904062 69 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Unknown Unknown Unknown YGL087C MMS2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0570995842833778 0.000935284289686514 70 Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress postreplication repair|response to DNA damage stimulus|protein polyubiquitination|free ubiquitin chain polymerization ubiquitin-protein ligase activity ubiquitin conjugating enzyme complex|nucleus|cytoplasm YLR252W_d YLR252W hom 0.0566724736058904 0.00102160070591123 71 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Unknown Unknown Unknown YDL187C_d YDL187C hom 0.0564939853363774 0.00105980933344 72 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL038C OCH1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0564132226479268 0.00107938687236224 73 Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins protein N-linked glycosylation alpha-1,6-mannosyltransferase activity|transferase activity, transferring glycosyl groups Golgi cis cisterna YML010W SPT5 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0563549982332139 0.00109047254621322 74 Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair 7-methylguanosine mRNA capping|intracellular mRNA localization|mRNA splicing, via spliceosome|positive regulation of transcription elongation from RNA polymerase II promoter|Unknown|regulation of transcription-coupled nucleotide-excision repair core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rRNA binding|RNA polymerase II core binding|RNA polymerase I transcription factor binding|RNA polymerase I core binding DSIF complex|mitochondrion|rDNA heterochromatin|nucleus YMR078C CTF18 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0560193554202042 0.00116793459060204 75 Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint maintenance of DNA trinucleotide repeats|double-strand break repair via homologous recombination|DNA replication initiation|mitotic sister chromatid cohesion molecular_function mitochondrion|nuclear replication fork|Ctf18 RFC-like complex YGR059W SPR3 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0560056928830403 0.00117119285034335 76 Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI ascospore formation structural molecule activity septin complex|ascospore wall|prospore membrane YLR290C_p YLR290C hom FT MITOCHONDRION 0.0555386448248326 0.00128775544069744 77 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene biological_process molecular_function mitochondrion YDR163W CWC15 hom FT RNA PROCESSING|NUCLEUS 0.054726507060414 0.00151624267967845 78 Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YDR376W ARH1 het FT KETONE METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0544915145957838 0.00158900301889753 79 Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability ubiquinone biosynthetic process|cellular iron ion homeostasis NADPH-adrenodoxin reductase activity mitochondrion|mitochondrial inner membrane YLR117C CLF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|NUCLEUS|CHROMOSOME 0.0543139662812073 0.0016460930042925 80 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins cis assembly of pre-catalytic spliceosome|DNA replication initiation DNA replication origin binding|chromatin binding|first spliceosomal transesterification activity|second spliceosomal transesterification activity chromatin|U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YDR034C LYS14 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0541287849777897 0.00171036854267747 81 Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YHR155W YSP1 hom FF|FT MITOCHONDRION 0.0540133612481514 0.00174706932949374 82 Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication biological_process molecular_function mitochondrion YNL290W RFC3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0538875873309583 0.00181379469452035 83 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity|ATPase activity Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|nucleus|Ctf18 RFC-like complex YGL260W_p YGL260W hom 0.053172579894831 0.00206366760051995 84 Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium biological_process molecular_function cellular_component YOR274W MOD5 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0524363186949768 0.00237643234408364 85 Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis tRNA modification tRNA dimethylallyltransferase activity cytosol|mitochondrion|nucleolus|nucleus YHR047C AAP1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0523636149797629 0.00240992311143295 86 Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication glycogen metabolic process|proteolysis aminopeptidase activity nucleus|cytoplasm YOR316C COT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0521946881113631 0.00248941254768248 87 Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication cellular zinc ion homeostasis|cobalt ion transport|zinc ion transport cobalt ion transmembrane transporter activity|zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|fungal-type vacuole YHL041W_d YHL041W hom 0.0521000206511213 0.00253500016525121 88 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YNL323W LEM3 hom FF|FT MEMBRANE ORGANIZATION|SIGNALING|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0515729895341379 0.00290290280130515 89 Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane phospholipid translocation|cell surface receptor signaling pathway phospholipid-translocating ATPase activity integral to membrane|endoplasmic reticulum|plasma membrane|cytoplasm YGL130W CEG1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0515258788527415 0.00282822091535478 90 Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 7-methylguanosine mRNA capping|positive regulation of transcription from RNA polymerase II promoter mRNA guanylyltransferase activity mRNA cap methyltransferase complex|nucleus YMR316C-B_d YMR316C-B hom 0.0509694386447508 0.00314162097531062 91 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR321W ASP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0504851856362268 0.00343977327980691 92 Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively asparagine catabolic process asparaginase activity intracellular YJR062C NTA1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0500388222366519 0.00373716396124607 93 Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation cellular protein modification process|protein catabolic process protein-N-terminal asparagine amidohydrolase activity mitochondrion YPR089W_p YPR089W hom FT GOLGI APPARATUS 0.0499979890319313 0.00376550317717173 94 Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p biological_process molecular_function cellular_component YHR122W CIA2 het FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS 0.0498892528082651 0.00384192337105114 95 Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B establishment of mitotic sister chromatid cohesion molecular_function nucleus|cytoplasm YOR298W MUM3 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS 0.0498752503160953 0.00385714963655998 96 Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases ascospore wall assembly|phospholipid biosynthetic process transferase activity, transferring acyl groups cellular_component YPL199C_p YPL199C hom 0.0498089308004125 0.00389927548891969 97 Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cytoplasm YKR044W UIP5 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.049414095497169 0.00419266126310071 98 Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates biological_process molecular_function nuclear envelope YAL034C-B_d YAL034C-B het 0.049363970899323 0.00423130486062953 99 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL053W AIM39 hom FT MITOCHONDRION 0.0492941640523601 0.00428566055389482 100 Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YGL221C NIF3 hom FT MITOCHONDRION 0.0492187621855807 0.00434508321660637 101 Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YNL308C KRI1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0487362146621846 0.00474337658763552 102 Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YLR361C DCR2 hom FT CELL CYCLE|SIGNALING 0.0484396350145174 0.00501078062100676 103 Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START endoplasmic reticulum unfolded protein response|traversing start control point of mitotic cell cycle|protein dephosphorylation hydrolase activity, acting on ester bonds|phosphoprotein phosphatase activity|phosphoric ester hydrolase activity cellular_component YNR005C_d YNR005C hom 0.0483942947300058 0.00504528467823363 104 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL014W_p YPL014W hom FT NUCLEUS 0.0480348294802312 0.0053813112684646 105 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YLR034C SMF3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0480003478703138 0.00541458337940503 106 Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress cellular iron ion homeostasis|iron ion transport metal ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YLR236C_d YLR236C hom 0.0479589098767189 0.00545481340339448 107 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL178W DLD2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.047747169897194 0.00566462159169564 108 D-lactate dehydrogenase, located in the mitochondrial matrix lactate metabolic process actin binding|D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial matrix YIL085C KTR7 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0476436998757819 0.00576977087514171 109 Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication fungal-type cell wall organization|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YPR138C MEP3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0475070124539853 0.00591137286595373 110 Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YGR207C CIR1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0472912407746924 0.00614128215563771 111 Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion YDL179W PCL9 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.0472836892555709 0.00614947228836789 112 Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p regulation of establishment or maintenance of cell polarity|regulation of transcription involved in G1/S phase of mitotic cell cycle cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|incipient cellular bud site|cellular bud neck YMR182C RGM1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|CHROMOSOME 0.04725433418223 0.00618140332401884 113 Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nuclear chromatin YNL056W OCA2 hom 0.0471753186236813 0.00626809539419483 114 Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene biological_process molecular_function cytoplasm YKL074C MUD2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0471516837097405 0.00629423820281377 115 Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 mRNA branch site recognition pre-mRNA branch point binding commitment complex YDR156W RPA14 hom FT NUCLEUS 0.0471016676125192 0.00634988520952505 116 RNA polymerase I subunit A14 transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YPL010W RET3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0470300285926046 0.00643036045609562 117 Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER molecular_function COPI vesicle coat YML065W ORC1 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.0468983906705746 0.00658062974302025 118 Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding|ATPase activity|ATP binding DNA replication preinitiation complex|nucleus|pre-replicative complex|nuclear origin of replication recognition complex YNR030W ALG12 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0468727295258458 0.00669983218684688 119 Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation alpha-1,6-mannosyltransferase activity integral to membrane|endoplasmic reticulum YDL053C PBP4 hom FT NUCLEUS 0.046735369515029 0.00677109499029867 120 Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasmic stress granule|cytoplasm YJL118W YJL118W hom 0.0466360851172098 0.00688950595066225 121 Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein biological_process molecular_function ribosome YGR152C RSR1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0463576229063522 0.00723159657641656 122 GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases bipolar cellular bud site selection|small GTPase mediated signal transduction|axial cellular bud site selection|cellular bud site selection GTPase activity|signal transducer activity plasma membrane|cellular bud neck YML028W TSA1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|TRANSLATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0463456937431281 0.00724658615084673 123 Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress protein folding|cellular response to oxidative stress|replicative cell aging|DNA protection|DNA damage checkpoint|cell redox homeostasis|response to hydroperoxide unfolded protein binding|ribosome binding|peroxiredoxin activity|thioredoxin peroxidase activity cytosol|polysome|cytoplasm YPL150W_p YPL150W hom FT PROTEIN PHOSPHORYLATION 0.0463400259094018 0.00725371782178745 124 Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study protein phosphorylation protein kinase activity cellular_component YKL077W_p YKL077W hom 0.0461537891099644 0.00749158428394575 125 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YJL097W PHS1 het FT KETONE METABOLISM|LIPID METABOLISM|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0461433762817908 0.007505087902733 126 Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking vacuolar transport|sphingolipid biosynthetic process enoyl-CoA hydratase activity fungal-type vacuole membrane|integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|fungal-type vacuole|nuclear outer membrane-endoplasmic reticulum membrane network YBL043W ECM13 hom FT CELL WALL ORG/BIOGENESIS 0.0460097385526308 0.00771674680284674 127 Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication fungal-type cell wall organization molecular_function cellular_component YOR150W MRPL23 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0458869094642329 0.00784464103825489 128 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YBR093C PHO5 hom FT RESPONSE TO NUTRIENT LEVELS 0.0458307487565495 0.00792080703854939 129 Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 cellular response to phosphate starvation|phosphate-containing compound metabolic process|regulation of cell size nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|acid phosphatase activity fungal-type cell wall|cell wall-bounded periplasmic space YGR206W MVB12 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|VACUOLAR TRANSPORT 0.0457554385050652 0.00802398290615737 130 ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin negative regulation of protein complex assembly|protein targeting to vacuole|endosome transport via multivesicular body sorting pathway ubiquitin binding cytosol|endosome|ESCRT I complex YIL011W TIR3 hom 0.0456817586623075 0.00815458518920944 131 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth biological_process molecular_function fungal-type cell wall YKL105C SEG2 hom 0.045151757065231 0.00889546077758205 132 Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes biological_process molecular_function cellular_component YGR142W BTN2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0447357886246947 0.00960872093602545 133 v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication intracellular protein transport|amino acid transport|retrograde transport, endosome to Golgi|regulation of pH SNARE binding cytosol|late endosome|perinuclear region of cytoplasm YLR024C UBR2 hom 0.0446898038099687 0.00961841570348365 134 Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YPL155C KIP2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.044664665673911 0.00965921819772312 135 Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle nuclear migration along microtubule|mitotic anaphase B|negative regulation of microtubule depolymerization microtubule motor activity mitochondrion|kinesin complex|cytoplasmic microtubule YPL015C HST2 hom FT GENE SILENCING|NUCLEUS 0.0446628112883135 0.00966223420839962 136 Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export negative regulation of chromatin silencing at telomere|chronological cell aging|chromatin silencing at rDNA|negative regulation of mitotic recombination NAD-dependent histone deacetylase activity cytoplasm YLR017W MEU1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0443165354489017 0.0102403491632186 137 Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression L-methionine salvage from methylthioadenosine|glutamate biosynthetic process S-methyl-5-thioadenosine phosphorylase activity|mRNA binding cytoplasm YFL030W AGX1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.044138950569873 0.0105486077234723 138 Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases glycine biosynthetic process, by transamination of glyoxylate alanine-glyoxylate transaminase activity mitochondrion YFL044C OTU1 hom FT PROTEOLYSIS|NUCLEUS 0.043944800514248 0.0109068912017425 139 Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress protein deubiquitination|regulation of transcription, DNA-dependent ubiquitin-specific protease activity nucleus|cytoplasm YDL100C GET3 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.043834864123664 0.0111076678769661 140 Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress retrograde vesicle-mediated transport, Golgi to ER|pheromone-dependent signal transduction involved in conjugation with cellular fusion|response to heat|protein insertion into ER membrane|response to metal ion|posttranslational protein targeting to membrane ATPase activity|guanyl-nucleotide exchange factor activity endoplasmic reticulum|GET complex YPL252C YAH1 het FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0438144986484825 0.0111331706627908 141 Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin ubiquinone biosynthetic process|iron-sulfur cluster assembly|heme a biosynthetic process oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor mitochondrial matrix YMR213W CEF1 het FT CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0429204001337534 0.0128955459777663 142 Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YDL138W RGT2 hom FT SIGNALING|PLASMA MEMBRANE 0.0428815743563255 0.0129773775215982 143 Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication glucose transport|signal transduction|detection of glucose glucose transmembrane transporter activity|receptor activity|glucose binding integral to membrane|plasma membrane YJR011C_p YJR011C hom 0.0426942157112539 0.0133787535842061 144 Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YGR125W_p YGR125W hom 0.04265741879779 0.0134588592730946 145 Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function integral to membrane|fungal-type vacuole YGL159W_p YGL159W hom 0.0425733787472764 0.0136995605294062 146 Putative protein of unknown function; deletion mutant has no detectable phenotype biological_process molecular_function cellular_component YBR053C_p YBR053C hom 0.042395277114581 0.0140562130555618 147 Putative protein of unknown function; induced by cell wall perturbation biological_process molecular_function cellular_component YAL046C AIM1 hom 0.0423686559036064 0.0141166957258372 148 Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YKL013C ARC19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0423181896868231 0.0142175344653597 149 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin cortical patch assembly structural molecule activity Arp2/3 protein complex YDR541C_p YDR541C hom FT OXIDATION-REDUCTION PROCESS 0.0421757064964092 0.0145472801101666 150 Putative dihydrokaempferol 4-reductase biological_process carbonyl reductase (NADPH) activity cellular_component YAL027W SAW1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0420467093082125 0.0148516096863093 151 5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus double-strand break repair via single-strand annealing, removal of nonhomologous ends molecular_function nucleus YJL186W MNN5 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0419757601106128 0.0150213715741184 152 Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YKL001C MET14 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0418251552952726 0.0153873999111254 153 Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process adenylylsulfate kinase activity cytoplasm YOR044W IRC23 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0416465059610676 0.0158317440570661 154 Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication biological_process molecular_function endoplasmic reticulum YMR048W CSM3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0416461601901243 0.0158326148971334 155 Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress DNA repair|DNA replication checkpoint|meiotic chromosome segregation|maintenance of DNA repeat elements|replication fork arrest|replication fork protection|mitotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion molecular_function replication fork protection complex|nucleus YGR122W YGR122W hom FF|FT TRANSCRIPTION FROM RNA POL II 0.0415779331912194 0.0160052737950173 156 Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function cytoplasm YKR013W PRY2 hom 0.0415717836527212 0.0160209171362405 157 Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication biological_process molecular_function extracellular region|fungal-type vacuole YOR186W_p YOR186W hom 0.0414639318814125 0.0162974654931581 158 Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication biological_process molecular_function cellular_component YHR113W APE4 hom FT PROTEOLYSIS 0.041376014080054 0.0165259931765574 159 Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family proteolysis aminopeptidase activity fungal-type vacuole lumen|ribosome|cytoplasm YHL001W RPL14B hom FT TRANSLATION|RIBOSOME 0.0412772530548563 0.0168514010946329 160 Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YGR261C APL6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0411594258327456 0.0171010190136603 161 Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools vesicle-mediated transport|Golgi to vacuole transport molecular_function AP-3 adaptor complex YFR051C RET2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0411509862451437 0.0171237763747403 162 Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER|Golgi inheritance|ER to Golgi vesicle-mediated transport|Golgi localization molecular_function COPI vesicle coat YDR513W GRX2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0411409138147263 0.0171509714573606 163 Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication cellular response to oxidative stress disulfide oxidoreductase activity|glutathione transferase activity|glutathione peroxidase activity cytosol|mitochondrion YNL327W EGT2 hom FT CELL CYCLE|CELL DIVISION 0.0411407006650595 0.0171515473602853 164 Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner cytokinesis cellulase activity fungal-type cell wall|cellular bud|cell septum YBR130C SHE3 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.041052837813403 0.017390390427592 165 Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance mating type switching|endoplasmic reticulum inheritance|intracellular mRNA localization mRNA binding actin cap|cytoplasm|cellular bud tip YHR200W RPN10 hom FT PROTEOLYSIS 0.0410218947281131 0.0174751963853839 166 Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein ubiquitin-dependent protein catabolic process structural molecule activity|polyubiquitin binding proteasome complex|proteasome regulatory particle, base subcomplex YPL156C PRM4 hom 0.0408858405585475 0.0178523958705895 167 Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function integral to membrane YMR204C INP1 hom FT PEROXISOME ORGANIZATION 0.040821812138576 0.0180495405687316 168 Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane peroxisome inheritance molecular_function peroxisome|extrinsic to intraperoxisomal membrane YOL084W PHM7 hom FT PLASMA MEMBRANE 0.0407665502351895 0.0181889661375371 169 Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|fungal-type vacuole YDL156W CMR1 hom FT NUCLEUS|CHROMOSOME 0.0407475807827087 0.0182429959440789 170 DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS biological_process DNA binding nuclear chromatin|nucleus|cytoplasm YML020W_p YML020W hom 0.0407091931831102 0.0183527634191191 171 Putative protein of unknown function biological_process molecular_function cellular_component YDL204W RTN2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.040619275536759 0.0186121433847252 172 Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum tubular network maintenance molecular_function endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YJL145W SFH5 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0404026174763755 0.0192503319460673 173 Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Golgi to plasma membrane protein transport|phosphatidylinositol metabolic process|Unknown|regulation of exocytosis|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cell periphery|cytosol|plasma membrane|cortical endoplasmic reticulum YOL063C CRT10 hom FT TRANSCRIPTION FROM RNA POL II 0.0404017135108765 0.0192530342258421 174 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YMR164C MSS11 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0403119392046576 0.0195230581264566 175 Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YGR174W-A_p YGR174W-A hom 0.0402680019226747 0.0197846789136577 176 Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis biological_process molecular_function cellular_component YMR192W GYL1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0401700675396346 0.019974992996849 177 Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress ER to Golgi vesicle-mediated transport|regulation of exocytosis protein binding mitochondrion|incipient cellular bud site|cellular bud|cellular bud neck|cellular bud tip|cytoplasm|Golgi-associated vesicle|plasma membrane YGL142C GPI10 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0398896248097628 0.0208381333027938 178 Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog GPI anchor biosynthetic process dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to membrane YGR168C_p YGR168C hom 0.0398115597171628 0.0210895004980214 179 Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function integral to membrane YFR040W SAP155 hom FT CELL CYCLE 0.0396811481648045 0.0215153140158928 180 Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity cytoplasm YDR059C UBC5 hom 0.0396736077958493 0.0216182553821124 181 Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication protein polyubiquitination|response to stress ubiquitin-protein ligase activity proteasome complex YGR241C YAP1802 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0396492599998962 0.0216205651416966 182 Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YDL215C GDH2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0394724821953198 0.0222920081685455 183 NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels nitrogen compound metabolic process glutamate dehydrogenase (NAD+) activity mitochondrion YGL109W_d YGL109W hom 0.0394082398834197 0.022430673791475 184 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Unknown Unknown Unknown YCL022C_d YCL022C hom 0.0393463764313368 0.0226428133164708 185 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Unknown Unknown Unknown YML115C VAN1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0391294176129106 0.0234006322401033 186 Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant protein N-linked glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YGL213C SKI8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0391028614659691 0.02349488546791 187 Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|protein complex assembly|nuclear-transcribed mRNA catabolic process, non-stop decay|protein-DNA complex assembly|reciprocal meiotic recombination molecular_function Ski complex|nuclear chromosome YNL067W RPL9B hom FT TRANSLATION|RIBOSOME 0.0390077856759871 0.0238350342132331 188 Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YGR266W YGR266W hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0389715515597773 0.023965786722287 189 Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrial outer membrane|mitochondrion|plasma membrane YJR039W_p YJR039W hom FT MITOCHONDRION 0.0389263862117336 0.0241296381757775 190 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YKL157W APE2 hom FT PROTEOLYSIS|MITOCHONDRION 0.0389063430174698 0.0242026615950162 191 Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication peptide catabolic process metalloaminopeptidase activity mitochondrion|extracellular region|cell wall-bounded periplasmic space YCL012C YCL012C hom 0.0388938329758077 0.0244396789692145 192 Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene biological_process molecular_function cellular_component YKR018C YKR018C hom FT NUCLEUS|MITOCHONDRION 0.0388591229162462 0.0243754561212533 193 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YGR088W CTT1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0388532026274573 0.0243971957012538 194 Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide response to reactive oxygen species catalase activity cytoplasm YJR019C TES1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0388345929825771 0.0247011704988097 195 Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids fatty acid beta-oxidation|fatty acid oxidation acyl-CoA hydrolase activity mitochondrion|peroxisome YDL065C PEX19 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0388327572247324 0.0245364170248975 196 Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning protein stabilization|protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization|protein exit from endoplasmic reticulum peroxisome membrane targeting sequence binding|protein binding cytosol|peroxisomal membrane|endoplasmic reticulum YLR255C_d YLR255C hom 0.0387662842307123 0.0247183028125361 197 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHL033C RPL8A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0387373101525578 0.02482615339365 198 Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YMR129W POM152 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|NUCLEAR PORE 0.0386820702570467 0.0250329026300199 199 Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 nucleocytoplasmic transport|nuclear pore organization|protein import into nucleus|spindle pole body duplication associated with nuclear envelope protein anchor|structural constituent of nuclear pore mitochondrion|integral to membrane|nuclear pore transmembrane ring|nuclear envelope lumen|nuclear pore YOL037C_d YOL037C hom 0.0386427013628429 0.0252029043992258 200 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Unknown Unknown Unknown YLR286C CTS1 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0386147264761833 0.0252869678771886 201 Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p cytokinesis, completion of separation|chitin catabolic process endochitinase activity nuclear envelope|extracellular region|fungal-type cell wall|endoplasmic reticulum|cellular bud neck YJL010C NOP9 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0385364625605793 0.0255850309036631 202 Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit export from nucleus RNA binding preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YAR031W PRM9 hom FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0385037140681337 0.0257768927670664 203 Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family vesicle organization molecular_function integral to membrane|endoplasmic reticulum|plasma membrane YJL048C UBX6 hom FT PROTEOLYSIS|NUCLEUS 0.0384332074714214 0.0259829157559085 204 UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication sporulation resulting in formation of a cellular spore|ubiquitin-dependent protein catabolic process molecular_function nucleus YNL096C RPS7B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.038322034397808 0.0264172789930402 205 Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosome biogenesis structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit YGL167C PMR1 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0382618640862512 0.026654974869257 206 High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease manganese ion transport|calcium ion transport|exocytosis manganese-transporting ATPase activity|calcium-transporting ATPase activity|calcium ion binding integral to membrane|Golgi membrane YER172C BRR2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0381951385131905 0.0269207234577807 207 RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) ATP-dependent RNA helicase activity U4/U6 x U5 tri-snRNP complex|U5 snRNP YNR051C BRE5 hom FT VESICLE-MEDIATED TRANSPORT 0.038110301467312 0.0272619039087895 208 Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A protein deubiquitination|ribophagy|regulation of ER to Golgi vesicle-mediated transport|Unknown molecular_function cytoplasm YPL116W HOS3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0380700761702817 0.0274249730532058 209 Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of transcription from RNA polymerase II promoter|histone deacetylation histone deacetylase activity nucleus|cellular bud neck|cytoplasm YKR056W TRM2 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE 0.0380642474898284 0.0274717483700652 210 tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair double-strand break repair|DNA repair|tRNA modification tRNA (m5U54) methyltransferase activity|double-stranded DNA specific 5'-3' exodeoxyribonuclease activity|single-stranded DNA specific endodeoxyribonuclease activity cellular_component YBL104C SEA4 hom 0.0380384911754161 0.0275536041839563 211 Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites biological_process molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane|cytoplasm YPL034W_p YPL034W hom 0.0380231494967326 0.0276162712379102 212 Putative protein of unknown function; YPL034W is not essential gene biological_process molecular_function cellular_component YOR020W-A_p YOR020W-A hom FT MITOCHONDRION 0.0379153780152789 0.028059964729335 213 Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOL008W COQ10 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.037841363196329 0.0283682279318555 214 Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes cellular respiration ubiquinone binding mitochondrion|mitochondrial inner membrane YOR101W RAS1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0378202990676991 0.0284801415730776 215 GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress adenylate cyclase-activating G-protein coupled receptor signaling pathway|replicative cell aging|Ras protein signal transduction GTPase activity plasma membrane YLR066W SPC3 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0377483270371498 0.0287598460284065 216 Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex YDL006W PTC1 hom FF|FT RNA PROCESSING|SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.037657859224523 0.0294106939042331 217 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation mitochondrion inheritance|inactivation of MAPK activity involved in osmosensory signaling pathway|pheromone-dependent signal transduction involved in conjugation with cellular fusion|tRNA splicing, via endonucleolytic cleavage and ligation|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YLR022C SDO1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0376526208809559 0.0291675492828492 218 Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes mature ribosome assembly molecular_function nucleus|preribosome, large subunit precursor|polysome|cytoplasm YFL011W HXT10 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0376522057865366 0.0291693283357523 219 Putative hexose transporter, expressed at low levels and expression is repressed by glucose hexose transport|transmembrane transport galactose transmembrane transporter activity|mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YGR208W SER2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0375437627905614 0.0297347003847122 220 Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source L-serine biosynthetic process phosphoserine phosphatase activity nucleus|cytoplasm YIL014W MNT3 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0375402439681306 0.0296526059357366 221 Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation protein O-linked glycosylation alpha-1,3-mannosyltransferase activity cellular_component YBR114W RAD16 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0374941897926876 0.029853385311847 222 Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex nucleotide-excision repair, DNA damage recognition|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|damaged DNA binding|DNA-dependent ATPase activity nucleotide-excision repair factor 4 complex|Cul3-RING ubiquitin ligase complex YDL155W CLB3 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.037471485078323 0.0299527987777388 223 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress G2/M transition of mitotic cell cycle|S phase of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation cyclin-dependent protein kinase regulator activity nucleus|cytoplasm YGR216C GPI1 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0374518194978915 0.0300391350306631 224 Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YDR399W HPT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0374101923523796 0.0302225928899907 225 Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome GMP salvage|IMP salvage hypoxanthine phosphoribosyltransferase activity nucleus|cytoplasm YBR037C SCO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0373614369124086 0.0304386892076228 226 Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication copper ion transport|response to redox state|protein complex assembly copper ion binding|thioredoxin peroxidase activity mitochondrion|mitochondrial inner membrane YPL128C TBF1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0372977736952665 0.0307228566915805 227 Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation telomere maintenance via telomere lengthening|negative regulation of chromatin silencing|snoRNA transcription from an RNA polymerase II promoter|negative regulation of telomere maintenance via telomerase RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|chromatin insulator sequence binding|telomeric DNA binding nuclear chromosome, telomeric region|nucleus YGL136C MRM2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0372844607526448 0.0307825674215038 228 Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ rRNA methylation rRNA (uridine-2'-O-)-methyltransferase activity mitochondrion YBR200W-A_p YBR200W-A hom 0.0372404356552506 0.0309807369839965 229 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YBR252W DUT1 het FT NUCLEOTIDE METABOLISM|NUCLEUS 0.037125612729658 0.0315788250742653 230 deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate pyrimidine deoxyribonucleoside triphosphate catabolic process|dUTP catabolic process|dITP catabolic process dITP diphosphatase activity|dUTP diphosphatase activity nucleus|cytoplasm YNL311C SKP2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.0370342024786527 0.0319236892711834 231 F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular protein catabolic process|regulation of sulfur amino acid metabolic process ubiquitin-protein ligase activity|protein binding, bridging ribosome|SCF ubiquitin ligase complex YDR147W EKI1 hom FT LIPID METABOLISM 0.0370005516908863 0.0320798601830975 232 Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolamine kinase activity|choline kinase activity cytoplasm YOL151W GRE2 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0368880928083726 0.0326065312654141 233 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress ergosterol metabolic process|filamentous growth methylglyoxal reductase (NADPH-dependent) activity|3-methylbutanol:NAD(P) oxidoreductase activity nucleus|cytoplasm YJR149W_p YJR149W hom FT OXIDATION-REDUCTION PROCESS 0.0367723249911957 0.0332351440419634 234 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YKR046C PET10 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0366994498078693 0.0335066146097331 235 Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange aerobic respiration molecular_function lipid particle YIR020W-A_d YIR020W-A hom 0.03668602212481 0.0335714850879151 236 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YNL159C ASI2 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0365602457497705 0.0341843541855847 237 Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals ubiquitin-dependent protein catabolic process molecular_function nuclear inner membrane YMR290W-A_d YMR290W-A het FF 0.0364985258200519 0.0344885756993905 238 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Unknown Unknown Unknown YBR225W_p YBR225W hom 0.0364791494699959 0.0345845585599421 239 Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components biological_process molecular_function cellular_component YCR102C_p YCR102C hom FT OXIDATION-REDUCTION PROCESS 0.0363654092594309 0.0354811764039127 240 Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to copper ion molecular_function cellular_component YHL015W-A_p YHL015W-A hom 0.0363460821392261 0.0353866736875896 241 Putative protein of unknown function biological_process molecular_function cellular_component YPR171W BSP1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0361849627701536 0.036236795559579 242 Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton actin cortical patch localization protein binding, bridging mating projection tip|membrane|cellular bud neck|actin cortical patch|cellular bud tip YGR124W ASN2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0361346905301983 0.0364129603786359 243 Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication asparagine biosynthetic process asparagine synthase (glutamine-hydrolyzing) activity cytoplasm YDR003W-A_p YDR003W-A hom 0.0360834560731165 0.0365951329582803 244 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YOL029C_p YOL029C hom 0.0360205889322801 0.0369235613954236 245 Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component YMR303C ADH2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.035983833675976 0.0371167367838949 246 Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 ethanol metabolic process|NADH oxidation|fermentation|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity cytoplasm YKR095W MLP1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0359369944236352 0.0373641566914899 247 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs protein localization to nuclear pore|poly(A)+ mRNA export from nucleus|protein import into nucleus|transcriptional activation by promoter-terminator looping|nuclear retention of unspliced pre-mRNA at the site of transcription|telomere tethering at nuclear periphery ribonucleoprotein complex binding nucleoplasm|nuclear envelope|integral to membrane|ribonucleoprotein complex|spindle pole body YMR158C-A_p YMR158C-A hom 0.0358135503838872 0.0380229588885585 248 Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene biological_process molecular_function cellular_component YGL008C PMA1 het FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.035660615779726 0.0388528185224774 249 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] proton transport|regulation of pH|transmembrane transport hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|membrane raft|plasma membrane YPL091W GLR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0356488820093503 0.0389171192280929 250 Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|protein glutathionylation glutathione-disulfide reductase activity mitochondrion|nucleus|cytoplasm YNR049C MSO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0356427909407891 0.0390965459080418 251 Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress cellular membrane fusion|ascospore-type prospore membrane assembly|vesicle docking involved in exocytosis molecular_function SNARE complex|cellular bud membrane|plasma membrane|prospore membrane|cellular bud neck|cellular bud tip YDR352W YPQ2 hom 0.0356241856929556 0.03914043728892 252 Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter biological_process molecular_function fungal-type vacuole membrane|integral to membrane YCL001W RER1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0356133334722766 0.0391124759446305 253 Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER protein retention in ER lumen|retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport molecular_function COPI-coated vesicle|integral to membrane|ER to Golgi transport vesicle YOL129W VPS68 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|MITOCHONDRION 0.0355675546739436 0.0393652787392301 254 Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria late endosome to vacuole transport via multivesicular body sorting pathway|protein targeting to vacuole molecular_function Vps55/Vps68 complex|mitochondrion|fungal-type vacuole membrane|integral to membrane YDL240W LRG1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0354828243462891 0.0398368414364249 255 Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis fungal-type cell wall biogenesis|small GTPase mediated signal transduction Rho GTPase activator activity mitochondrion|cellular bud neck|cytoplasm YBL017C PEP1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0354579965779479 0.0399759235531965 256 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments vacuolar transport|protein targeting to vacuole signal sequence binding integral to membrane|Golgi apparatus YPR054W SMK1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.0354488472151918 0.040027280887046 257 Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p ascospore wall assembly|protein phosphorylation|negative regulation of sporulation resulting in formation of a cellular spore MAP kinase activity mitochondrion YGR178C PBP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS|MITOCHONDRION 0.0354404680897154 0.0400743637825376 258 Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress positive regulation of translation|mRNA polyadenylation|stress granule assembly molecular_function mitochondrion|nucleus|polysome|cytoplasmic stress granule|cytoplasm YML019W OST6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0353731491073019 0.0404543407336286 259 Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p protein N-linked glycosylation via asparagine|protein complex assembly dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YJL050W MTR4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|OXIDATION-REDUCTION PROCESS|RNA LOCALIZATION|NUCLEUS 0.0353588496004089 0.0405354452073795 260 ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent 3'-5' RNA helicase activity|poly(A) RNA binding nucleolus|nucleus|TRAMP complex YJR079W_p YJR079W hom 0.0353442906360134 0.0409489931269788 261 Putative protein of unknown function; mutation results in impaired mitochondrial respiration biological_process molecular_function cellular_component YDL201W TRM8 hom FT RNA PROCESSING|NUCLEUS 0.0353441954327074 0.0406187041742621 262 Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YOL124C TRM11 hom FT RNA PROCESSING 0.0352149337351912 0.0413594119922361 263 Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain tRNA methylation S-adenosylmethionine-dependent methyltransferase activity|RNA binding|tRNA (guanine-N2-)-methyltransferase activity cytoplasm|tRNA (m2G10) methyltransferase complex YDL222C FMP45 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0349336273586237 0.0431978088893754 264 Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C fungal-type cell wall organization|ascospore formation molecular_function cell cortex|mitochondrion|integral to membrane|plasma membrane YNL247W YNL247W het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0347900339079024 0.0438752725549254 265 Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments cysteinyl-tRNA aminoacylation cysteine-tRNA ligase activity ribosome|cytoplasm YDR134C YDR134C hom 0.0347723019374084 0.0440145394226971 266 Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication biological_process molecular_function fungal-type cell wall YDL224C WHI4 hom 0.0345592808294129 0.0452948844297626 267 Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication regulation of cell size RNA binding cytoplasm YGR132C PHB1 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0345519764607244 0.0455659322655494 268 Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation mitochondrion inheritance|protein folding|negative regulation of proteolysis|replicative cell aging|mitochondrion morphogenesis|inner mitochondrial membrane organization molecular_function mitochondrion|mitochondrial inner membrane YDR238C SEC26 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0345431609160021 0.0453954854162683 269 Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) ER to Golgi vesicle-mediated transport molecular_function coated vesicle|COPI vesicle coat YBL048W_d RRT1 hom 0.0344934742245789 0.0457390586717869 270 Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YKL063C_p YKL063C hom FT GOLGI APPARATUS 0.0344144671741023 0.0462054058772857 271 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi biological_process molecular_function Golgi apparatus YLR076C_d YLR076C het 0.0343696162582278 0.0471150560645796 272 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Unknown Unknown Unknown YKR054C DYN1 hom FT CELL CYCLE|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0343297408102417 0.046745234516442 273 Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p nuclear migration along microtubule|establishment of mitotic spindle orientation|mitotic spindle elongation|mitotic sister chromatid segregation microtubule motor activity cytoplasmic dynein complex|cell cortex|cytoplasmic microtubule|spindle pole body|astral microtubule YNR021W_p YNR021W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0343168999377174 0.0468603111726823 274 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene biological_process molecular_function endoplasmic reticulum YLL020C_d YLL020C hom 0.0343137417109571 0.0468477649483847 275 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Unknown Unknown Unknown YIL155C GUT2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0342206284877003 0.0474482428132353 276 Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner NADH oxidation|replicative cell aging|glycerol metabolic process glycerol-3-phosphate dehydrogenase activity mitochondrion|integral to mitochondrial outer membrane YER057C HMF1 hom FT NUCLEUS 0.0341467059965333 0.0479295559531619 277 Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro biological_process molecular_function cytosol|nucleus|cytoplasm YDR461W MFA1 hom FT SIGNALING|PLASMA MEMBRANE 0.034123142638021 0.0483848635307406 278 Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 pheromone-dependent signal transduction involved in conjugation with cellular fusion mating pheromone activity extracellular region YNL178W RPS3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0340708566542213 0.0484276711636311 279 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome|DNA-(apurinic or apyrimidinic site) lyase activity preribosome, small subunit precursor|cytosolic small ribosomal subunit|90S preribosome YDL035C GPR1 hom FT SIGNALING|PLASMA MEMBRANE 0.034063001268906 0.0484795062412804 280 Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis replicative cell aging|G-protein coupled receptor signaling pathway|sucrose mediated signaling|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|invasive growth in response to glucose limitation|detection of glucose|detection of sucrose stimulus G-protein coupled receptor activity|glucose binding integral to membrane|plasma membrane YOL022C TSR4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS 0.0340318984739886 0.0486852007559533 281 Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 maturation of SSU-rRNA molecular_function cytoplasm YDL160C DHH1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|RNA LOCALIZATION 0.0339909656628242 0.0489907467712962 282 Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|cell morphogenesis involved in conjugation with cellular fusion|stress granule assembly|cytoplasmic mRNA processing body assembly protein binding|RNA helicase activity cytoplasmic mRNA processing body|cytoplasmic stress granule|cytoplasm YKL156W RPS27A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0339743772912702 0.0491013135002992 283 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasm YOR172W YRM1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0339625270329621 0.0491466200146812 284 Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|nucleic acid binding transcription factor activity nucleus|cytoplasm YER162C RAD4 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0339038921748769 0.0495394810248774 285 Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins proteasomal ubiquitin-dependent protein catabolic process|nucleotide-excision repair damaged DNA binding cytosol|nucleotide-excision repair factor 2 complex|nucleus YGR141W VPS62 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.0338686937756589 0.0497765770388984 286 Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins protein targeting to vacuole molecular_function cellular_component YPR005C HAL1 hom FT TRANSCRIPTION FROM RNA POL II 0.033816656256289 0.0501288432129509 287 Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p response to salt stress|positive regulation of transcription from RNA polymerase II promoter molecular_function cytoplasm YJL208C NUC1 hom FT NUCLEUS|MITOCHONDRION 0.0337493948791662 0.0505872593749382 288 Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy DNA catabolic process|RNA catabolic process|apoptotic process|DNA recombination ribonuclease activity|endodeoxyribonuclease activity|exodeoxyribonuclease activity mitochondrion|mitochondrial inner membrane|nucleus YBR147W RTC2 hom FT MITOCHONDRION 0.0334975783830041 0.0523347899587266 289 Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter biological_process molecular_function mitochondrion|integral to membrane YLR078C BOS1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0334578357833853 0.0526151489597209 290 v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|ER to Golgi transport vesicle membrane|endoplasmic reticulum membrane YDR500C RPL37B hom FT TRANSLATION|RIBOSOME 0.0334276411907922 0.0528643405417471 291 Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR372W SUR4 hom FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0333154256969415 0.0536300872774287 292 Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis post-Golgi vesicle-mediated transport|fatty acid elongation|fatty acid biosynthetic process|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum YIL025C_d YIL025C hom 0.0332768428478834 0.053907858577525 293 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR037C SPC34 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0332709980511859 0.054345301156993 294 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|DASH complex|condensed nuclear chromosome kinetochore|spindle pole body|spindle YKR055W RHO4 hom FT SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0332262520498314 0.0542738971884234 295 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity positive regulation of formin-nucleated actin cable assembly|maintenance of cell polarity|regulation of formin-nucleated actin cable assembly GTPase activity incipient cellular bud site|plasma membrane|cellular bud neck YIL038C NOT3 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0331425378590671 0.0548841447780192 296 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|positive regulation of transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|cytoplasm YGR003W CUL3 hom FT PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0330942708617948 0.0552749106392617 297 Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity nucleus|Cul3-RING ubiquitin ligase complex|cytoplasm YLR304C ACO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0330903277692704 0.0552676260082752 298 Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy propionate metabolic process|glutamate biosynthetic process|tricarboxylic acid cycle|mitochondrial genome maintenance|citrate metabolic process double-stranded DNA binding|aconitate hydratase activity|single-stranded DNA binding mitochondrial nucleoid|cytosol|mitochondrion|mitochondrial matrix YNL217W_p YNL217W hom 0.0330641345083322 0.0554608538716594 299 Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate biological_process phosphatase activity fungal-type vacuole YPL228W CET1 het FT RNA PROCESSING|NUCLEUS 0.0330313588366849 0.0557034324862397 300 Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 7-methylguanosine mRNA capping polynucleotide 5'-phosphatase activity mRNA cap methyltransferase complex|nucleus YDR286C_p YDR286C hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0330080170216087 0.0558767275692836 301 Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component YDR086C SSS1 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0329178664642157 0.0565502461539404 302 Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation structural molecule activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex|Ssh1 translocon complex YBR274W CHK1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0327959835481794 0.057471564469246 303 Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase protein phosphorylation|replication fork protection|DNA damage checkpoint protein serine/threonine kinase activity nucleus YLR179C YLR179C hom FT NUCLEUS 0.0327490640970731 0.0578295405289015 304 Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YIL103W DPH1 hom 0.032723774353117 0.0581730830583855 305 Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YHL002W HSE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0326330771156732 0.0587224294526176 306 Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT-0 complex YIR014W_p YIR014W hom 0.0325988001615156 0.0589884797409277 307 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene biological_process molecular_function fungal-type vacuole YNL190W_p YNL190W hom 0.032552546298432 0.0593490769463145 308 Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site cellular response to water deprivation molecular_function fungal-type cell wall YHR193C EGD2 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.0325208495249851 0.05963526188417 309 Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes 'de novo' cotranslational protein folding unfolded protein binding|phosphatidic acid binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding nascent polypeptide-associated complex YKL096C-B_p YKL096C-B hom 0.0324020394793404 0.0605351105171633 310 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YFR016C YFR016C hom 0.0323727027681633 0.0607685631984496 311 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene biological_process molecular_function cellular bud|cytoplasm YGR092W DBF2 hom FF|FT CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0323261326316535 0.0611792880832435 312 Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis nuclear division|protein phosphorylation|vacuolar acidification protein serine/threonine kinase activity|protein kinase activity spindle pole body|cellular bud neck YML047C PRM6 hom 0.0322968383944501 0.0613757281035213 313 Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family biological_process molecular_function integral to membrane YBL076C ILS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0321796497726277 0.0623625272119219 314 Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A cytoplasmic translation|isoleucyl-tRNA aminoacylation isoleucine-tRNA ligase activity cytosol YMR215W GAS3 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0320566551020384 0.0633311808816762 315 Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation biological_process 1,3-beta-glucanosyltransferase activity fungal-type cell wall|integral to membrane|plasma membrane YLR101C_d YLR101C het 0.0319808168100526 0.0639592047839425 316 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W Unknown Unknown Unknown YJR128W_d YJR128W hom 0.0319576472376599 0.0641520962013687 317 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Unknown Unknown Unknown YNL095C_p YNL095C hom 0.0319309672462739 0.0643748070194376 318 Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication biological_process molecular_function integral to membrane YGR143W SKN1 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0319011233678447 0.0646246838236487 319 Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p fungal-type cell wall organization|(1->6)-beta-D-glucan biosynthetic process|sphingolipid biosynthetic process glucosidase activity integral to membrane YEL053C MAK10 hom 0.0318431362989698 0.0651124854779948 320 Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatC complex|cytoplasm YNL047C SLM2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0318307610331936 0.0661051429364768 321 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication eisosome assembly|TOR signaling cascade|regulation of cell growth|actin filament bundle assembly|endosomal transport|establishment or maintenance of actin cytoskeleton polarity|actin cytoskeleton organization phosphatidylinositol binding plasma membrane YKL185W ASH1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0318200395394089 0.0653076246819684 322 Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|core promoter proximal region sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|cellular bud|Rpd3L complex YML101C CUE4 hom FT ENDOPLASMIC RETICULUM 0.0318150115423503 0.0653501689023571 323 Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication biological_process molecular_function endoplasmic reticulum|cytoplasm YGR227W DIE2 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0317745684719259 0.0656932080418869 324 Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 dolichol-linked oligosaccharide biosynthetic process|protein N-linked glycosylation dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YDR167W TAF10 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.0317641495236672 0.0657818217720478 325 Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|protein complex scaffold SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YLR177W_p YLR177W hom 0.0317630745037586 0.0657909704778482 326 Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication biological_process molecular_function cytoplasm YIL120W QDR1 hom FT PLASMA MEMBRANE 0.0317606462620897 0.0658116393194946 327 Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication drug transmembrane transport drug transmembrane transporter activity integral to membrane|plasma membrane YKR024C DBP7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0317065005507398 0.0664765225523446 328 Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YPR026W ATH1 hom FT CARBOHYDRATE METABOLISM 0.0316866793203284 0.0664438030284639 329 Acid trehalase required for utilization of extracellular trehalose trehalose catabolic process|response to stress alpha,alpha-trehalase activity fungal-type cell wall|cell wall-bounded periplasmic space|fungal-type vacuole YBR249C ARO4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0316190751557948 0.0670259516306081 330 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity nucleus|cytoplasm YIL142W CCT2 het 0.0315955148250235 0.0672298164013973 331 Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YMR107W SPG4 hom 0.0315814824001791 0.0673514795211053 332 Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component YOR013W_d IRC11 hom 0.0314966787368225 0.0682139976015396 333 Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YLR118C YLR118C hom FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.0314896159785728 0.0681524554462198 334 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS protein deacylation palmitoyl-(protein) hydrolase activity nucleus|cytoplasm YKL043W PHD1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0314669498970673 0.0683512787055739 335 Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication pseudohyphal growth|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YKL205W LOS1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0313977038457057 0.0690859499500393 336 Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm tRNA re-export from nucleus|tRNA splicing, via endonucleolytic cleavage and ligation|tRNA export from nucleus tRNA binding|Ran GTPase binding mitochondrion|integral to membrane|nuclear matrix|cytoplasm YDR266C HEL2 hom 0.0313831421893054 0.0690905698982313 337 RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor biological_process molecular_function ribosome|cytoplasm YBR101C FES1 hom FT TRANSLATION|RIBOSOME 0.031377652673128 0.0691392226903823 338 Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress cytoplasmic translation adenyl-nucleotide exchange factor activity cytosol|cytosolic ribosome YDR284C DPP1 hom FT LIPID METABOLISM|SIGNALING 0.0312711790601933 0.0700884601223667 339 Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism phospholipid metabolic process|signal transduction diacylglycerol diphosphate phosphatase activity|phosphatidate phosphatase activity fungal-type vacuole membrane|integral to membrane YER098W UBP9 hom FT PROTEOLYSIS 0.0312123439091884 0.0706175585231384 340 Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication protein deubiquitination ubiquitin-specific protease activity cytoplasm YFR057W_p YFR057W hom 0.0311780040629571 0.0709699606908913 341 Putative protein of unknown function biological_process molecular_function cellular_component YJR052W RAD7 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0311023206183989 0.0718275820353068 342 Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex nucleotide-excision repair, DNA damage recognition|protein ubiquitination involved in ubiquitin-dependent protein catabolic process damaged DNA binding|ubiquitin-protein ligase activity|DNA-dependent ATPase activity nucleotide-excision repair factor 4 complex|Cul3-RING ubiquitin ligase complex YDR425W SNX41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT 0.0311011649913684 0.0717533342263338 343 Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p retrograde transport, endosome to Golgi phosphatidylinositol binding|phosphatidylinositol-3-phosphate binding endosome YDL242W_d YDL242W hom 0.0310188806342912 0.0723804843413432 344 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR094W VAS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0310117997000314 0.0724456862074494 345 Mitochondrial and cytoplasmic valyl-tRNA synthetase valyl-tRNA aminoacylation valine-tRNA ligase activity mitochondrion|cytoplasm YLR090W XDJ1 hom FT NUCLEUS|MITOCHONDRION 0.0309983774775315 0.0725694111184086 346 Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding chaperone binding mitochondrion|integral to mitochondrial outer membrane|nucleus YPL145C KES1 hom FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0308983851425707 0.0734965832920379 347 One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication post-Golgi vesicle-mediated transport|positive regulation of phosphatase activity|maintenance of cell polarity|endocytosis|exocytosis|sterol transport phosphatidic acid binding|lipid binding|phosphatidylinositol-4,5-bisphosphate binding|oxysterol binding|sterol transporter activity|phosphatidylinositol-4-phosphate binding Golgi membrane|extrinsic to membrane|cytoplasm YPL200W CSM4 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CHROMOSOME 0.0308219837355074 0.0742115176342124 348 Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements meiotic telomere clustering|meiotic chromosome segregation|synapsis|regulation of reciprocal meiotic recombination molecular_function nuclear chromosome, telomeric region|endoplasmic reticulum membrane YDR485C VPS72 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0307853858450334 0.074555993328547 349 Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting chromatin remodeling|histone exchange histone binding Swr1 complex|nucleus|cytoplasm YLL028W TPO1 hom FT PLASMA MEMBRANE 0.0307294773327995 0.0750847492262625 350 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane spermidine transport|putrescine transport|spermine transport spermidine transmembrane transporter activity|spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|cellular bud membrane|plasma membrane YBL029W_p YBL029W hom FT NUCLEUS 0.0305762530109496 0.0765495754079451 351 Non-essential protein of unknown function biological_process molecular_function nucleus|cytoplasm YDR224C HTB1 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0305534929950562 0.0767691350276678 352 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation chromatin assembly or disassembly|negative regulation of transcription from RNA polymerase II promoter|postreplication repair DNA binding replication fork protection complex|nuclear nucleosome YPR058W YMC1 hom FT MITOCHONDRION 0.0305483232748079 0.0771276857539841 353 Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication mitochondrial transport organic acid transmembrane transporter activity mitochondrion|integral to membrane YJR034W PET191 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0304778978199797 0.077502066029244 354 Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|integral to mitochondrial inner membrane YCR048W ARE1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0304519690797872 0.077932134625329 355 Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication sterol metabolic process ergosterol O-acyltransferase activity|lanosterol O-acyltransferase activity integral to membrane|endoplasmic reticulum YDR262W_p YDR262W hom 0.0304300711433283 0.0779687038751873 356 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment biological_process molecular_function fungal-type vacuole YHR132C ECM14 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS 0.0302922354493863 0.0793263501626033 357 Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly fungal-type cell wall organization|proteolysis metalloendopeptidase activity fungal-type vacuole YFL017W-A SMX2 het FT RNA PROCESSING|NUCLEUS 0.0302686013105375 0.0795610589068243 358 Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YJR070C LIA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CYTOSKELETON ORGANIZATION|NUCLEUS 0.0302556756130113 0.0798244575190159 359 Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress microtubule cytoskeleton organization|peptidyl-lysine modification to hypusine deoxyhypusine monooxygenase activity nucleus|cytoplasm YPL086C ELP3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION|NUCLEUS 0.0301946076158836 0.0802995356316219 360 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification histone acetyltransferase activity Elongator holoenzyme complex|nucleus|cytoplasm YDR094W_d YDR094W hom 0.0301793425172015 0.0804525758342912 361 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Unknown Unknown Unknown YER015W FAA2 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0301270983857764 0.080978142246068 362 Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome long-chain fatty acid metabolic process long-chain fatty acid-CoA ligase activity|very long-chain fatty acid-CoA ligase activity|medium-chain fatty acid-CoA ligase activity mitochondrion|peroxisome YML087C AIM33 hom FT OXIDATION-REDUCTION PROCESS 0.030120242953091 0.0810473129631148 363 Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL162C_p YPL162C hom 0.0300721465693005 0.0815339504351643 364 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function fungal-type vacuole membrane|integral to membrane YJR090C GRR1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON|UBIQUITIN LIGASE COMPLEX 0.0300624890175654 0.0816319503295678 365 F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury|cell cycle arrest in response to pheromone|G1/S transition of mitotic cell cycle|response to DNA damage stimulus|protein ubiquitination ubiquitin-protein ligase activity|protein binding, bridging cellular bud neck contractile ring|SCF ubiquitin ligase complex|nucleus|cytoplasm YLR217W_d YLR217W hom 0.0299949039831712 0.0824576476436795 366 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Unknown Unknown Unknown YNL270C ALP1 hom FT PLASMA MEMBRANE 0.0299895110206738 0.0824213091155623 367 Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication basic amino acid transport basic amino acid transmembrane transporter activity integral to membrane|plasma membrane YOL024W_p YOL024W hom 0.029980236721309 0.0824704814210669 368 Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication biological_process molecular_function cellular_component YGR193C PDX1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0298742860104801 0.0836069581796921 369 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core acetyl-CoA biosynthetic process from pyruvate structural molecule activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YPL064C CWC27 hom FT RNA PROCESSING|NUCLEUS 0.0298390344243362 0.0839262511578828 370 Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress biological_process molecular_function U2-type spliceosomal complex YOR045W TOM6 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0297914861847898 0.0844211194194445 371 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial matrix protein channel activity mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YBR215W HPC2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0297899352929389 0.0844373003111856 372 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding HIR complex|nucleus YNL023C FAP1 hom FT NUCLEUS 0.0297810757587724 0.0845297824265658 373 Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 biological_process sequence-specific DNA binding transcription factor activity cytoplasm YDL011C_d YDL011C hom 0.0297758023719721 0.0850032880815185 374 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Unknown Unknown Unknown YPL147W PXA1 hom FT NUCLEOTIDE METABOLISM 0.0297741054189539 0.0846026013525731 375 Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder fatty acid transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|integral to peroxisomal membrane YBR133C HSL7 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0297725984238159 0.0846183515519218 376 Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress G2/M transition of mitotic cell cycle|regulation of cell cycle protein-arginine omega-N symmetric methyltransferase activity|protein-arginine omega-N monomethyltransferase activity cellular bud neck YMR006C PLB2 hom FT LIPID METABOLISM 0.0297208653568226 0.0851604735623762 377 Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine glycerophospholipid metabolic process lysophospholipase activity extracellular region|fungal-type cell wall YER059W PCL6 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0296614530638072 0.0858800201288143 378 Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding regulation of glycogen catabolic process|regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YBR236C ABD1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|RNA POL II, HOLOENZYME 0.0296611170430986 0.0857900786570429 379 Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA 7-methylguanosine mRNA capping|transcription from RNA polymerase II promoter mRNA (guanine-N7-)-methyltransferase activity nucleus|DNA-directed RNA polymerase II, holoenzyme YDR240C SNU56 het FT RNA PROCESSING|NUCLEUS 0.0296454878880007 0.0859553916925932 380 Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YML070W DAK1 hom FT CARBOHYDRATE METABOLISM 0.0296217162364558 0.0862073228513619 381 Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation response to stress|glycerol catabolic process glycerone kinase activity cytoplasm YHR046C INM1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|NUCLEUS 0.0296210051820377 0.0862148677557219 382 Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate inositol phosphate dephosphorylation inositol monophosphate 1-phosphatase activity nucleus|cytoplasm YOR358W HAP5 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0295981360388118 0.0864578136740029 383 Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex YPL274W SAM3 hom FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0294433134186447 0.088117110562112 384 High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p spermidine transport|putrescine transport|S-adenosylmethionine transport S-adenosylmethionine transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YLR300W EXG1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0293669575364736 0.0889448470198572 385 Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes fungal-type cell wall organization|cellular glucan metabolic process glucan exo-1,3-beta-glucosidase activity extracellular region|fungal-type cell wall YDR487C RIB3 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0293171838099126 0.0894877826994988 386 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration riboflavin biosynthetic process|aerobic respiration 3,4-dihydroxy-2-butanone-4-phosphate synthase activity cytosol|mitochondrial intermembrane space YDR192C NUP42 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0292174090762061 0.0905841657010302 387 FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|mRNA export from nucleus in response to heat stress|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YBR092C PHO3 hom 0.0291493381520581 0.0913383479334225 388 Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin phosphate-containing compound metabolic process|thiamine-containing compound metabolic process acid phosphatase activity cell wall-bounded periplasmic space YJL164C TPK1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0291247744496505 0.0916117336825755 389 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication protein phosphorylation|Ras protein signal transduction cAMP-dependent protein kinase activity|protein kinase activity cAMP-dependent protein kinase complex|nucleus|cytoplasm YER064C VHR2 hom FT NUCLEUS 0.029106131231243 0.0918196648936766 390 Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress regulation of transcription, DNA-dependent molecular_function nucleus YAL031C GIP4 hom FT CHROMOSOME SEGREGATION 0.0290946416606559 0.0919479988355072 391 Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate chromosome segregation|regulation of phosphoprotein phosphatase activity protein phosphatase 1 binding|protein phosphatase type 1 regulator activity cytoplasm YGL010W_p YGL010W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0290437367223083 0.0925183206010782 392 Putative protein of unknown function; YGL010W is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YDR246W TRS23 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.02900963755953 0.0929019416031754 393 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 chromosome organization|ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YHR022C_p YHR022C hom FT SIGNALING 0.0289785720486737 0.0932525435426377 394 Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component YOR006C TSR3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0289560939505715 0.0935068900312396 395 Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress maturation of SSU-rRNA molecular_function nucleus|cytoplasm YPR076W_d YPR076W hom 0.0289536236740622 0.0935837816679831 396 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL139C YUR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0289473050599249 0.093606490143357 397 Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication cell wall mannoprotein biosynthetic process|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YGR051C_d YGR051C hom 0.0289321991653039 0.0939248741390616 398 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Unknown Unknown Unknown YMR015C ERG5 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0288997157500554 0.0941472742307252 399 C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs ergosterol biosynthetic process C-22 sterol desaturase activity endoplasmic reticulum YCR082W AHC2 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0288786563316856 0.0943873820295433 400 Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex|nucleus|cytoplasm YOR049C RSB1 hom FT MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0288070216746494 0.095207795535137 401 Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane phospholipid translocation|fatty acid transport phospholipid-translocating ATPase activity integral to membrane|endoplasmic reticulum|plasma membrane YCR019W MAK32 hom 0.0287375625498153 0.0960087352892345 402 Protein necessary for structural stability of L-A double-stranded RNA-containing particles interspecies interaction between organisms molecular_function cellular_component YDR275W BSC2 hom 0.028699457435992 0.0964504134074973 403 Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function lipid particle YER018C SPC25 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0286200075250167 0.0973765464470974 404 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YPR168W NUT2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0285557354382998 0.0981309417515341 405 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YKL036C_d YKL036C het 0.0285096152779187 0.0986751484983846 406 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Unknown Unknown Unknown YGL055W OLE1 het FF|FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.028488802470147 0.0989215220061256 407 Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria unsaturated fatty acid biosynthetic process electron carrier activity|stearoyl-CoA 9-desaturase activity integral to endoplasmic reticulum membrane|integral to membrane YAR002C-A ERP1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0284702985662976 0.0993931627497967 408 Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|ER to Golgi transport vesicle YFR027W ECO1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0284487370859218 0.0993971812831539 409 Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress double-strand break repair|regulation of DNA replication|mitotic chromosome condensation|internal peptidyl-lysine acetylation|establishment of mitotic sister chromatid cohesion|DNA repair|chromosome organization|DNA replication|regulation of mitosis|telomere organization|tRNA gene clustering acetyltransferase activity|chromatin binding nuclear chromatin|nuclear replication fork YPL120W VPS30 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.028446717696304 0.099471657076891 410 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 phosphatidylinositol biosynthetic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|peroxisome degradation|retrograde transport, endosome to Golgi|macroautophagy|CVT pathway|late endosome to vacuole transport 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|extrinsic to membrane|cytoplasm YLR247C IRC20 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0284464361346917 0.0994245536402332 411 Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci double-strand break repair via synthesis-dependent strand annealing helicase activity mitochondrion|nucleus YNL141W AAH1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0283353146165961 0.100753637385495 412 Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome adenine catabolic process|hypoxanthine salvage|purine-containing compound salvage adenine deaminase activity nucleus|cytoplasm YKL090W CUE2 hom 0.0283322607669059 0.100790362193294 413 Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination biological_process ubiquitin binding cellular_component YGL096W TOS8 hom FT NUCLEUS|CHROMOSOME 0.0283230477030034 0.100901220726555 414 Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication biological_process chromatin binding|sequence-specific DNA binding nuclear chromatin YDL127W PCL2 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0282341322201995 0.101976116663119 415 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth G1 phase of mitotic cell cycle|regulation of establishment or maintenance of cell polarity|regulation of cyclin-dependent protein kinase activity|septin ring organization cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|incipient cellular bud site|nucleus|cellular bud neck|cellular bud tip YMR252C_p YMR252C hom FT MITOCHONDRION 0.028215502505951 0.102253636078365 416 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion YOR181W LAS17 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0282088272748011 0.102334862280406 417 Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) bipolar cellular bud site selection|endocytosis|actin polymerization or depolymerization|actin filament organization|actin cortical patch localization|positive regulation of actin filament bundle assembly|response to osmotic stress|cytokinesis cytoskeletal protein binding mating projection tip|cytoplasm|actin cortical patch YIL039W TED1 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0281455656542908 0.103055831761928 418 Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum YER175C TMT1 hom 0.0281166479897519 0.103410328224542 419 Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle biological_process trans-aconitate 3-methyltransferase activity cytosol YPR072W NOT5 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0280938094177532 0.103690986411952 420 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|cytoplasm YOR206W NOC2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0280626633891852 0.104333076476683 421 Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors ribosomal subunit export from nucleus|ribosome assembly molecular_function mitochondrion|nucleus|Noc2p-Noc3p complex|Noc1p-Noc2p complex YML013W UBX2 hom FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.0280509291201217 0.104219567218481 422 Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis proteasomal ubiquitin-dependent protein catabolic process|ER-associated protein catabolic process|lipid particle organization protein binding, bridging mitochondrial outer membrane|Doa10p ubiquitin ligase complex|integral to endoplasmic reticulum membrane|lipid particle|endoplasmic reticulum YFR025C HIS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.028019730241668 0.104709017763755 423 Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control histidine biosynthetic process histidinol-phosphatase activity intracellular YDR184C ATC1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS 0.0280107373806745 0.104716946727062 424 Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress bipolar cellular bud site selection|response to stress molecular_function nucleus YLR405W DUS4 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS 0.0280012701570825 0.105041758938473 425 Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p tRNA modification tRNA dihydrouridine synthase activity cellular_component YER187W_p YER187W hom 0.0279747709724007 0.105163632524083 426 Putative protein of unknown function; induced in respiratory-deficient cells biological_process molecular_function cellular_component YMR167W MLH1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0279742688547237 0.105169879270182 427 Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer mismatch repair|meiotic mismatch repair|reciprocal meiotic recombination|meiotic heteroduplex formation double-stranded DNA binding|DNA insertion or deletion binding|loop DNA binding|single-stranded DNA binding|dinucleotide insertion or deletion binding|ATPase activity|ATP binding mitochondrion|nucleus|MutLalpha complex|MutLbeta complex|cytoplasm YMR171C EAR1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0279631772256175 0.105307943103284 428 Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function endosome YLR035C MLH2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0279523917673546 0.105442333680927 429 Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p mismatch repair|reciprocal meiotic recombination molecular_function nucleus YPL044C_d YPL044C het 0.0279236027281076 0.10585375834514 430 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Unknown Unknown Unknown YPL227C ALG5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0278653855319046 0.106531442270921 431 UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein N-linked glycosylation dolichyl-phosphate beta-glucosyltransferase activity endoplasmic reticulum membrane YGR144W THI4 hom FT MITOCHONDRION ORGANIZATION|NUCLEUS 0.0278056429087057 0.107284423566332 432 Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents thiamine biosynthetic process|mitochondrial genome maintenance|thiazole biosynthetic process ferrous iron binding cytosol YNL098C RAS2 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|RESPONSE TO NUTRIENT LEVELS|PLASMA MEMBRANE 0.0277975668727816 0.107386534463632 433 GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes pseudohyphal growth|ascospore formation|activation of adenylate cyclase activity|regulation of protein localization|replicative cell aging|positive regulation of transcription by galactose|Ras protein signal transduction GTP binding|GTPase activity plasma membrane YGR087C PDC6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0277571153550891 0.107899149290861 434 Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation L-phenylalanine catabolic process|ethanol metabolic process|tryptophan catabolic process|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway pyruvate decarboxylase activity cytoplasm YKL070W_p YKL070W hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0277350620115513 0.10839001399339 435 Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YKR038C KAE1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|PROTEOLYSIS|NUCLEUS|CHROMOSOME 0.0276687140110063 0.109026139119581 436 Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription chromosome organization|threonylcarbamoyladenosine metabolic process|telomere maintenance|positive regulation of transcription from RNA polymerase II promoter chromatin DNA binding nuclear chromatin|EKC/KEOPS complex|nucleus|cytoplasm YPL046C ELC1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0276320365868339 0.109602298451217 437 Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair global genome nucleotide-excision repair|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YNL084C END3 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0275995912674052 0.10991382074874 438 EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p bipolar cellular bud site selection|ascospore wall assembly|endocytosis|actin filament organization|cytokinesis|actin cortical patch assembly protein binding, bridging mating projection tip|actin cortical patch YOL162W_p YOL162W hom 0.027561805799192 0.110401473518 439 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YGL101W_p YGL101W hom FT NUCLEUS 0.0275508512041077 0.110543170591067 440 Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p biological_process molecular_function nucleus|cytoplasm YOR170W_d YOR170W hom 0.027531628613243 0.110792160687121 441 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Unknown Unknown Unknown YER062C HOR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0275231557624297 0.110902049925741 442 Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication cellular carbohydrate metabolic process|glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YJR124C_p YJR124C hom 0.027505876027557 0.111126427249893 443 Putative protein of unknown function; expression induced under calcium shortage biological_process molecular_function integral to membrane YKL140W TGL1 hom FT LIPID METABOLISM 0.0274392555224271 0.111994848558722 444 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes cellular lipid metabolic process|sterol metabolic process lipase activity|sterol esterase activity integral to membrane|lipid particle YIL010W DOT5 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0274073774263973 0.112412278856703 445 Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth cellular response to oxidative stress|cell redox homeostasis thioredoxin peroxidase activity nucleus YAL029C MYO4 hom FT RNA LOCALIZATION|MITOCHONDRION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0273831969960912 0.112729728483903 446 Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication mating type switching|endoplasmic reticulum inheritance|intracellular mRNA localization microfilament motor activity|actin filament binding mitochondrion|filamentous actin|myosin V complex|cellular bud|cellular bud tip YNL032W SIW14 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0273725620369036 0.112869571606887 447 Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm actin filament organization|endocytosis|protein dephosphorylation|response to stress protein tyrosine phosphatase activity cytoplasm YLR129W DIP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0273102772951829 0.113691323179784 448 Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YJR080C AIM24 hom FT MITOCHONDRION 0.0273092673946876 0.113704685964084 449 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YLL015W BPT1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS 0.0272788801712384 0.11416130474398 450 ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p cadmium ion transport|bilirubin transport bilirubin transmembrane transporter activity|cadmium ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YDR054C CDC34 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.027225372651626 0.114819086860619 451 Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein autoubiquitination|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle|protein polyubiquitination ubiquitin-protein ligase activity|protein homodimerization activity SCF ubiquitin ligase complex|nucleus|cytoplasm YDR006C SOK1 hom FT SIGNALING|NUCLEUS 0.0272027074023666 0.115121625632987 452 Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 cAMP-mediated signaling molecular_function nucleus YDL227C HO hom FT NUCLEUS 0.0271787769864056 0.115441731822289 453 Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p mating type switching|gene conversion at mating-type locus, DNA double-strand break formation endonuclease activity nucleus YFR021W ATG18 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|VACUOLAR PROTEIN PROCESSING 0.0271481911661263 0.115851882283637 454 Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood piecemeal microautophagy of nucleus|peroxisome degradation|vacuolar protein processing|macroautophagy|late endosome to vacuole transport|CVT pathway ubiquitin binding|phosphatidylinositol binding cytosol|endosome|fungal-type vacuole membrane|PAS complex|pre-autophagosomal structure YMR322C_p SNO4 hom FT PROTEOLYSIS 0.0270692330959177 0.116915989396895 455 Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation biological_process cysteine-type peptidase activity cellular_component YDR354W TRP4 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0270521095908902 0.117805215401408 456 Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis tryptophan biosynthetic process anthranilate phosphoribosyltransferase activity nucleus|cytoplasm YDR511W ACN9 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0270491839505408 0.117460891259403 457 Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes carbon utilization|regulation of gluconeogenesis molecular_function mitochondrion|mitochondrial intermembrane space YLR136C TIS11 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 0.0270419442197594 0.117285536593713 458 mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress cellular iron ion homeostasis|nuclear-transcribed mRNA catabolic process mRNA binding nucleus|cytoplasm YMR143W RPS16A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0269100721429011 0.119084285817015 459 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YPL251W_d YPL251W het 0.02688639657013 0.119409499509649 460 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Unknown Unknown Unknown YML046W PRP39 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0268649562745087 0.119704608651568 461 U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats mRNA 5'-splice site recognition pre-mRNA 5'-splice site binding commitment complex|U1 snRNP|U2-type prespliceosome YDR191W HST4 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0268215476927573 0.120303842517967 462 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|chromatin silencing at telomere|histone deacetylation histone deacetylase activity|DNA binding nucleus|cytoplasm YDR101C ARX1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|PROTEOLYSIS|NUCLEUS|RIBOSOME 0.0268172553638634 0.120418542629497 463 Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex ribosomal large subunit biogenesis molecular_function nucleoplasm|cytosolic large ribosomal subunit|cytoplasm YMR190C SGS1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0267686318897872 0.12103749084231 464 Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress DNA double-strand break processing|meiotic DNA double-strand break processing|telomeric 3' overhang formation|meiotic chromosome segregation|DNA unwinding involved in replication|double-strand break repair via homologous recombination|intra-S DNA damage checkpoint|telomere maintenance via recombination|replicative cell aging|DNA duplex unwinding|response to DNA damage stimulus|negative regulation of meiotic joint molecule formation|chromosome organization|regulation of reciprocal meiotic recombination|gene conversion at mating-type locus, DNA double-strand break processing|mitotic sister chromatid segregation ATP-dependent DNA helicase activity RecQ helicase-Topo III complex|nucleolus YCL061C MRC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0267664297088534 0.121068098529427 465 S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres maintenance of DNA repeat elements|intra-S DNA damage checkpoint|DNA repair|DNA replication checkpoint|telomere maintenance|replication fork protection|DNA replication|mitotic sister chromatid cohesion|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere molecular_function nuclear chromosome|replication fork protection complex|nucleus|nuclear replication fork YNL055C POR1 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0267325787176028 0.121539348906168 466 Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication DNA transport|mitochondrion organization|apoptotic process|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YJR040W GEF1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0267110367602508 0.12183998710245 467 Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism cellular copper ion homeostasis|cellular iron ion homeostasis voltage-gated chloride channel activity endosome|integral to membrane|endoplasmic reticulum|Golgi apparatus|plasma membrane|fungal-type vacuole|Golgi medial cisterna YDR520C_p URC2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM|NUCLEUS 0.0266721689026877 0.122607027275427 468 Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism uracil catabolic process sequence-specific DNA binding nucleus|cytoplasm YDL211C_p YDL211C hom 0.0266705392124 0.122574062791262 469 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YDR148C KGD2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0266700121306085 0.122637289063613 470 Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated tricarboxylic acid cycle|mitochondrial genome maintenance|2-oxoglutarate metabolic process dihydrolipoyllysine-residue succinyltransferase activity mitochondrial nucleoid|mitochondrion|mitochondrial oxoglutarate dehydrogenase complex YOR166C SWT1 hom FT NUCLEUS 0.0266665044444923 0.122463319476243 471 RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain nuclear mRNA surveillance of mRNP export|transcription, DNA-dependent endoribonuclease activity nucleus|cytoplasm YJL002C OST1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0265746974237505 0.123756228247911 472 Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation via asparagine|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YOL058W ARG1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0265429692343627 0.12420552083764 473 Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate argininosuccinate metabolic process|arginine biosynthetic process|citrulline metabolic process argininosuccinate synthase activity cytosol YLR323C CWC24 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0265220358883663 0.124502646945852 474 Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p generation of catalytic spliceosome for first transesterification step|snoRNA splicing molecular_function nucleus|U2-type spliceosomal complex YMR172W HOT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0264917123722624 0.125215630522687 475 Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p cellular hyperosmotic salinity response|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YDL062W_d YDL062W hom 0.0264682809639075 0.125268178382101 476 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Unknown Unknown Unknown YLR109W AHP1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0264538940752056 0.125642881561042 477 Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p cellular response to oxidative stress|response to metal ion|cell redox homeostasis thioredoxin peroxidase activity plasma membrane|cytoplasm YPL273W SAM4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0264299075709258 0.125816898993889 478 S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio homocysteine metabolic process|L-methionine biosynthetic process from S-adenosylmethionine|S-adenosylhomocysteine metabolic process homocysteine S-methyltransferase activity nucleus|cytoplasm YIR011C STS1 het FT PROTEIN LOCALIZATION|PROTEOLYSIS|CHROMOSOME SEGREGATION|NUCLEUS 0.0263825014905979 0.126610543976844 479 Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation proteasome localization|chromosome segregation|nucleus-associated proteasomal ubiquitin-dependent protein catabolic process molecular_function nucleus YHR049W FSH1 hom FT NUCLEUS 0.0263549280645919 0.126894468779962 480 Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process hydrolase activity nucleus|cytoplasm YNL305C BXI1 hom FT SIGNALING|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0263476673219726 0.126999196864332 481 Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p endoplasmic reticulum unfolded protein response|apoptotic process|calcium-mediated signaling molecular_function mitochondrion|integral to membrane|endoplasmic reticulum|fungal-type vacuole YLR165C PUS5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.02630333358499 0.127640120699571 482 Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA modification|pseudouridine synthesis pseudouridylate synthase activity mitochondrion YKL208W CBT1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0262757367074616 0.128496182984695 483 Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p cytochrome complex assembly|mRNA processing|mRNA stabilization molecular_function mitochondrion YHR127W YHR127W hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS 0.026269371169439 0.128132807357255 484 Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis mitotic spindle elongation molecular_function nucleus YDL172C_d YDL172C hom 0.0262512256358889 0.128396646419734 485 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL172W APC1 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|CYTOSKELETON|UBIQUITIN LIGASE COMPLEX 0.0262238821153875 0.128795022751601 486 Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress mitotic metaphase/anaphase transition|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|exit from mitosis|cyclin catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YPL189C-A COA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0261822064331408 0.1294040546562 487 Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p mitochondrial respiratory chain complex IV assembly molecular_function mitochondrial matrix YLL004W ORC3 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.0261801744085824 0.129433806917925 488 Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YER106W MAM1 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0260901869054493 0.130756707221576 489 Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle meiotic chromosome segregation|meiotic sister chromatid cohesion involved in meiosis I molecular_function condensed nuclear chromosome kinetochore|monopolin complex YLR193C UPS1 hom FT LIPID METABOLISM|MITOCHONDRION 0.0260728939714113 0.131012126164632 490 Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Unknown|cardiolipin metabolic process molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial intermembrane space YNR002C ATO2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0260561292682988 0.13126011211035 491 Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication ammonium transport|nitrogen utilization ammonium transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YLR360W VPS38 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0260508774364202 0.13133787276089 492 Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated ER-associated protein catabolic process|late endosome to vacuole transport molecular_function phosphatidylinositol 3-kinase complex II YDR387C_p YDR387C hom 0.0260363289144274 0.131553470216928 493 Putative transporter, member of the sugar porter family; YDR387C is not an essential gene transport|transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YJR048W CYC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0259954965069749 0.132160038699444 494 Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen|mitochondrial electron transport, ubiquinol to cytochrome c electron carrier activity mitochondrion|mitochondrial intermembrane space YAL045C_d YAL045C hom 0.0259909536562227 0.132227656750156 495 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Unknown Unknown Unknown YLL047W_d YLL047W hom 0.025979648917969 0.132396038372176 496 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Unknown Unknown Unknown YOR057W SGT1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|SIGNALING|UBIQUITIN LIGASE COMPLEX 0.0259101825392459 0.133434371600934 497 Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress regulation of cell cycle|kinetochore assembly|cAMP-mediated signaling|protein complex assembly|protein ubiquitination protein binding, bridging|chaperone binding ubiquitin ligase complex YLR046C_p YLR046C hom 0.0258696266727702 0.134043477023265 498 Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function integral to membrane YJL169W_d YJL169W hom 0.0258644131914693 0.134121933569175 499 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Unknown Unknown Unknown YNR027W BUD17 hom FT CELL CYCLE|CELL DIVISION|COFACTOR METABOLISM|NUCLEUS 0.0258587298334149 0.134207501624826 500 Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) pyridoxal phosphate biosynthetic process|cellular bud site selection pyridoxal kinase activity nucleus|cytoplasm YPL135W ISU1 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0258237685378776 0.13473480379803 501 Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable cellular iron ion homeostasis|iron-sulfur cluster assembly|tRNA wobble uridine modification ferrous iron binding mitochondrion|mitochondrial matrix YKR100C SKG1 hom FT CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0258204045973703 0.134843788140905 502 Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell fungal-type cell wall organization molecular_function incipient cellular bud site|cellular bud|cellular bud tip YOR002W ALG6 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0257635868074612 0.135646239442129 503 Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation|aerobic respiration transferase activity, transferring hexosyl groups|dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum YOL164W-A_p YOL164W-A hom 0.0257242643782615 0.136302763654171 504 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR356W CIR2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0257132518359045 0.13664621089562 505 Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response biological_process molecular_function mitochondrion YLL051C FRE6 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0257076722740371 0.136555784853738 506 Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels intracellular sequestering of iron ion|copper ion import ferric-chelate reductase activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YPL058C PDR12 hom FT KETONE METABOLISM|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0256875082496626 0.136805231132553 507 Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity propionate metabolic process|transport|organic acid transport xenobiotic-transporting ATPase activity|organic acid transmembrane transporter activity integral to membrane|plasma membrane YMR157C AIM36 hom FT MITOCHONDRION 0.0256606435564235 0.137216311298802 508 Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YPL054W LEE1 hom 0.0256475659195003 0.137416767963501 509 Zinc-finger protein of unknown function biological_process molecular_function cellular_component YER049W TPA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0256377483188646 0.138155364627057 510 Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|translational termination poly(A) RNA binding nucleus YJR035W RAD26 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0255462985509625 0.138976664066778 511 Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein transcription-coupled nucleotide-excision repair|nucleotide-excision repair DNA-dependent ATPase activity nucleus|cytoplasm YHR059W FYV4 hom FT MITOCHONDRION 0.0255147864656891 0.139464838349664 512 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function mitochondrion YJL151C SNA3 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.0254847740197244 0.139931006406151 513 Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles cellular protein catabolic process|endosome transport via multivesicular body sorting pathway ubiquitin protein ligase binding fungal-type vacuole lumen|integral to membrane YOR171C LCB4 hom FT LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0254448638178816 0.140552766522738 514 Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes calcium-mediated signaling|sphingolipid metabolic process D-erythro-sphingosine kinase activity Golgi apparatus|endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum YDL237W AIM6 hom 0.0254436176886461 0.140572214074577 515 Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene biological_process molecular_function cellular_component YKL107W_p YKL107W hom FT OXIDATION-REDUCTION PROCESS 0.0254434621297512 0.140574641929198 516 Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein biological_process molecular_function cellular_component YOR102W_d YOR102W het 0.0254393860866099 0.140638269452356 517 Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Unknown Unknown Unknown YPR117W_p YPR117W hom 0.0253818185863973 0.141717465759347 518 Putative protein of unknown function biological_process molecular_function cellular_component YNL150W_d YNL150W het 0.0253807448396557 0.141556118228531 519 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Unknown Unknown Unknown YML111W BUL2 hom FT UBIQUITIN LIGASE COMPLEX 0.0253787071947937 0.141588093815297 520 Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication protein polyubiquitination|protein monoubiquitination ubiquitin-ubiquitin ligase activity ubiquitin ligase complex|cytoplasm YBR141C_p YBR141C hom FT NUCLEUS 0.025371301196135 0.141704358605791 521 Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity nucleolus YHR180W_d YHR180W hom 0.0253636573191511 0.141824434652512 522 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER046W SPO73 hom FT CELL WALL ORG/BIOGENESIS 0.0253499766974596 0.142039535662548 523 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function cytosol YDR185C UPS3 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0253205840146598 0.142502525068521 524 Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations mitochondrion morphogenesis molecular_function extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial intermembrane space YBR172C SMY2 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0253106554984873 0.142659179045659 525 Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum membrane|cytoplasmic mRNA processing body|cytoplasm|extrinsic to membrane YMR183C SSO2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0253073356938585 0.142711589007647 526 Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Golgi to plasma membrane transport|ascospore-type prospore assembly|vesicle fusion phosphatidic acid binding|SNAP receptor activity endoplasmic reticulum|plasma membrane|prospore membrane|cytoplasm YBR162W-A YSY6 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0252431667931502 0.143727532226118 527 Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion protein secretion molecular_function endoplasmic reticulum YDR066C RTR2 hom FT NUCLEUS 0.0252404641548154 0.144069485276052 528 Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication biological_process molecular_function cytoplasm YER081W SER3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0252155147915199 0.14440647987341 529 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication serine family amino acid biosynthetic process phosphoglycerate dehydrogenase activity cytoplasm YLR008C PAM18 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0252128520867993 0.144209408680005 530 Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p protein import into mitochondrial matrix unfolded protein binding|ATPase activator activity mitochondrion|presequence translocase-associated import motor|mitochondrial inner membrane presequence translocase complex YLR100W ERG27 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0251707118337788 0.144881316832705 531 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs ergosterol biosynthetic process 3-keto sterol reductase activity mitochondrial outer membrane|endoplasmic reticulum|endoplasmic reticulum membrane YJR051W OSM1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0251319221891877 0.145561961306159 532 Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity mitochondrion YOL109W ZEO1 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0251314086516436 0.14551014733345 533 Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria fungal-type cell wall organization|response to stress molecular_function mitochondrial outer membrane|mitochondrion|extrinsic to plasma membrane|plasma membrane YMR023C MSS1 hom FT RNA PROCESSING|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0251156508365606 0.145762850030541 534 Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 mitochondrial tRNA wobble uridine modification|Unknown GTP binding mitochondrion|mitochondrial inner membrane YMR197C VTI1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0251139265132732 0.145790522785266 535 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Golgi to vacuole transport|vacuole fusion, non-autophagic|intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to Golgi membrane YBL040C ERD2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0251097548804753 0.146158281958837 536 HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins protein retention in ER lumen|ER to Golgi vesicle-mediated transport HDEL sequence binding integral to endoplasmic reticulum membrane|integral to membrane YJR152W DAL5 hom FT PLASMA MEMBRANE 0.0251076023154821 0.146132653345106 537 Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression dipeptide transport|allantoate transport dipeptide transporter activity|allantoate transmembrane transporter activity integral to membrane|plasma membrane YJL161W_p FMP33 hom FT MITOCHONDRION 0.0251055735122418 0.145924632597982 538 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YGR114C_d YGR114C het FF 0.0250868187118699 0.147070934199087 539 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Unknown Unknown Unknown YGL108C YGL108C hom 0.0250606019402271 0.146889389806553 540 Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress biological_process molecular_function cellular_component YHR094C HXT1 hom FT PLASMA MEMBRANE 0.025057705629594 0.146694985990195 541 Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication mannose transport|glucose transport galactose transmembrane transporter activity|pentose transmembrane transporter activity|mannose transmembrane transporter activity|glucose transmembrane transporter activity|fructose transmembrane transporter activity integral to membrane|plasma membrane YFR038W IRC5 hom FT NUCLEUS 0.0250466759357916 0.146872930417446 542 Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci mitotic recombination molecular_function cellular_component YCR004C YCP4 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0250466427214845 0.146873466520104 543 Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process electron carrier activity mitochondrion|membrane raft|plasma membrane|cytoplasm YOR008C-A_p YOR008C-A hom 0.025025766468625 0.147391783420999 544 Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres biological_process molecular_function cellular_component YDL200C MGT1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0249600332738824 0.14845808433578 545 DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage DNA dealkylation involved in DNA repair methylated-DNA-[protein]-cysteine S-methyltransferase activity nucleus YDL120W YFH1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0249362200290391 0.148664050337834 546 Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia cellular iron ion homeostasis|iron-sulfur cluster assembly|glutathione metabolic process ferrous iron binding|ferroxidase activity|iron chaperone activity mitochondrion|mitochondrial matrix YPL263C KEL3 hom 0.0249313144942089 0.148743981967295 547 Cytoplasmic protein of unknown function biological_process molecular_function cytoplasm YGR226C_d YGR226C hom 0.0248720556606323 0.149712148588046 548 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Unknown Unknown Unknown YGR068C ART5 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0248660567784172 0.149810425135825 549 Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane ubiquitin-dependent endocytosis ubiquitin protein ligase binding cellular_component YHR014W SPO13 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0248596846607258 0.149914870072804 550 Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II protein localization to chromosome, centromeric region|meiotic sister chromatid cohesion|positive regulation of sister chromatid cohesion molecular_function nucleus|condensed nuclear chromosome, centromeric region YFR009W GCN20 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0248141357651216 0.150967774817285 551 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA regulation of translational elongation ATPase activity cytosol|cytosolic ribosome YGR160W_d YGR160W hom 0.0247968818831872 0.150947237935108 552 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR148W YSW1 hom FT MEMBRANE ORGANIZATION 0.0247940055404937 0.150994649347515 553 Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane ascospore-type prospore membrane assembly molecular_function prospore membrane YML131W YML131W hom FT OXIDATION-REDUCTION PROCESS 0.0247401388813978 0.151884642438706 554 Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm YLR366W_d YLR366W hom 0.0247161931037107 0.152342696943387 555 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Unknown Unknown Unknown YML027W YOX1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0246933677275655 0.152660636033671 556 Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication negative regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II regulatory region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nuclear chromatin|nucleus YGR239C PEX21 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.024677569855709 0.152923423202147 557 Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress protein import into peroxisome matrix|positive regulation of protein binding protein binding cytosol|peroxisome YDL078C MDH3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.0246338454229705 0.153652543787767 558 Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle glyoxylate cycle|fatty acid beta-oxidation|malate metabolic process|NADH regeneration L-malate dehydrogenase activity|mRNA binding peroxisomal matrix|peroxisome YLR343W GAS2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0246290041910122 0.154471648178449 559 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p ascospore wall assembly 1,3-beta-glucanosyltransferase activity integral to membrane|cytoplasm YLR103C CDC45 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.024622537427982 0.153841537932866 560 DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes S phase of mitotic cell cycle|regulation of chromatin silencing at telomere|DNA replication initiation|pre-replicative complex assembly|double-strand break repair via break-induced replication DNA replication origin binding|chromatin binding replication fork protection complex|nucleus|DNA replication preinitiation complex|nuclear replication fork|pre-replicative complex YPL219W PCL8 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0245996248722871 0.154225024281305 561 Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation negative regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YDR095C_d YDR095C hom 0.0245923559221829 0.154777632882284 562 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR025W CSI1 hom FT SIGNALING|NUCLEUS 0.0245242010589303 0.155492518739109 563 Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YLR348C DIC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0245201312444256 0.155931417575859 564 Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix dicarboxylic acid transport dicarboxylic acid transmembrane transporter activity mitochondrial envelope|mitochondrion|integral to membrane YML077W BET5 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0245106453324039 0.155721157753341 565 Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YPL025C_d YPL025C hom 0.0244893750242569 0.156080429433834 566 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL068W NUP100 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0244884096594033 0.156096750079508 567 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p positive regulation of transcription, DNA-dependent|mRNA export from nucleus|NLS-bearing substrate import into nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|protein import into nucleus|protein targeting to nuclear inner membrane|mRNA export from nucleus in response to heat stress nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YLR173W_p YLR173W hom 0.0244876003601099 0.156110433247717 568 Putative protein of unknown function biological_process molecular_function cellular_component YJR006W POL31 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0244848960693601 0.156156162442067 569 Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair mismatch repair|RNA-dependent DNA replication|lagging strand elongation|base-excision repair|postreplication repair|DNA replication, removal of RNA primer|leading strand elongation|nucleotide-excision repair single-stranded DNA specific 3'-5' exodeoxyribonuclease activity|DNA-directed DNA polymerase activity delta DNA polymerase complex YBR216C YBP1 hom FT RESPONSE TO OXIDATIVE STRESS 0.0244710689109993 0.15645190599133 570 Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication cellular response to oxidative stress molecular_function cytoplasm YFL013C IES1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0244486595610197 0.157265285285506 571 Subunit of the INO80 chromatin remodeling complex nucleosome mobilization molecular_function nucleus|Ino80 complex YDR116C MRPL1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0244468569776789 0.156800475147507 572 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR045C STU2 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.02440639473894 0.157488040770939 573 Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p microtubule nucleation|mitotic spindle organization in nucleus microtubule binding|structural constituent of cytoskeleton cell cortex|spindle microtubule|condensed nuclear chromosome kinetochore|spindle pole body|kinetochore YOL017W ESC8 hom FT GENE SILENCING|NUCLEUS 0.0243398932269411 0.158623043105138 574 Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication chromatin silencing molecular_function nucleus YDR466W PKH3 hom FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION 0.0243212134111978 0.158942968214259 575 Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant MAPK cascade involved in cell wall biogenesis|protein phosphorylation protein kinase activity cellular_component YNL131W TOM22 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0242740573778961 0.15975277040425 576 Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes protein import into mitochondrial matrix|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YDR441C APT2 hom 0.0242622438699042 0.159956129375751 577 Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication biological_process adenine phosphoribosyltransferase activity|molecular_function cytoplasm YOR376W_d YOR376W hom 0.0242574863369223 0.160038081288698 578 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Unknown Unknown Unknown YBR138C_p YBR138C hom 0.0242269615933238 0.160564646942121 579 Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm YKL040C NFU1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0241991748996379 0.161045116945596 580 Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria cellular iron ion homeostasis|iron-sulfur cluster assembly|intracellular sequestering of iron ion molecular_function mitochondrion|mitochondrial matrix YOR284W HUA2 hom 0.0241512612302324 0.161876158649679 581 Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YKR059W TIF1 hom FT TRANSLATION|RIBOSOME 0.0241211918574306 0.162461996279044 582 Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication translational initiation ATP-dependent RNA helicase activity|translation initiation factor activity ribosome|eukaryotic translation initiation factor 4F complex YLL061W MMP1 hom FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0240775669122552 0.163160664279814 583 High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p S-methylmethionine transport S-methylmethionine transmembrane transporter activity integral to membrane|plasma membrane YML051W GAL80 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEUS 0.0240648764958615 0.163445420079643 584 Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding negative regulation of kinase activity|galactose metabolic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity RNA polymerase II activating transcription factor binding|kinase inhibitor activity nucleus|cytoplasm YGR204C-A_p YGR204C-A hom 0.0239837783021529 0.164806508055671 585 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPL007C TFC8 het FT NUCLEUS 0.0239774399641389 0.164918185110395 586 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter|transcription from RNA polymerase III promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YDL221W_d YDL221W het 0.0239733252106581 0.165053725139679 587 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Unknown Unknown Unknown YER065C ICL1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.023968882642963 0.165069049417443 588 Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose glyoxylate cycle isocitrate lyase activity cellular_component YMR056C AAC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0239543729175632 0.165388147675795 589 Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress heme biosynthetic process|mitochondrial transport|heme transport|aerobic respiration ATP:ADP antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YCL069W VBA3 hom 0.0239496752408051 0.165471119266368 590 Permease of basic amino acids in the vacuolar membrane basic amino acid transport|transmembrane transport basic amino acid transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YBL073W_d YBL073W het 0.0238995136333016 0.16629584973994 591 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Unknown Unknown Unknown YNL183C NPR1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|GOLGI APPARATUS|PLASMA MEMBRANE 0.0238929919288506 0.16641153846821 592 Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex regulation of transmembrane transporter activity|regulation of nitrogen utilization|protein phosphorylation|negative regulation of endocytosis protein serine/threonine kinase activity Golgi apparatus|plasma membrane|cytoplasm YJR094W-A RPL43B hom FT TRANSLATION|RIBOSOME 0.0238409974334868 0.167336034836151 593 Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR277C YSH1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0238121330406173 0.167850924857154 594 Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication termination of RNA polymerase II transcription|mRNA cleavage|mRNA polyadenylation|RNA splicing|snoRNA splicing|snoRNA 3'-end processing endoribonuclease activity|RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YOR142W LSC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0237991370686549 0.168146569837883 595 Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated tricarboxylic acid cycle|succinyl-CoA metabolic process succinate-CoA ligase (ADP-forming) activity mitochondrial nucleoid|mitochondrion YBR065C ECM2 hom FT RNA PROCESSING|NUCLEUS 0.0237666614148173 0.168664469741443 596 Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p mRNA splicing, via spliceosome molecular_function nucleus YGL137W SEC27 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0237474156517195 0.169009690245013 597 Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP retrograde vesicle-mediated transport, Golgi to ER|late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YGR049W SCM4 hom FT MITOCHONDRION 0.0237086421200434 0.169706798302443 598 Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to membrane YAR015W ADE1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0237045217883603 0.169844661782588 599 N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolesuccinocarboxamide synthase activity nucleus|cytoplasm YER046W-A_d YER046W-A hom 0.0236955691251487 0.169942322331923 600 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR082C SRL2 hom FT NUCLEUS 0.0236880627073977 0.170077669140155 601 Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation nucleobase-containing compound metabolic process molecular_function nucleus|cytoplasm YLL002W RTT109 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0236587444647911 0.170926179530095 602 Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p negative regulation of transposition, RNA-mediated|histone acetylation|double-strand break repair via nonhomologous end joining|response to DNA damage stimulus|regulation of transcription from RNA polymerase II promoter in response to stress H3 histone acetyltransferase activity nucleus YOR288C MPD1 hom FT ENDOPLASMIC RETICULUM 0.023645286865965 0.170850493983605 603 Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation protein folding protein disulfide oxidoreductase activity|protein disulfide isomerase activity fungal-type vacuole YOR162C YRR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0236327758437964 0.171077024956609 604 Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter in response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YOR190W SPR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0236065398295776 0.171552797609478 605 Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance ascospore formation glucan exo-1,3-beta-glucosidase activity fungal-type cell wall|ascospore wall YIL023C YKE4 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0235311483713879 0.172925480803624 606 Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family zinc ion transport zinc ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YPR106W ISR1 hom FT PROTEIN PHOSPHORYLATION 0.0235014751551854 0.173467998540172 607 Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C biological_process protein kinase activity cellular_component YMR320W_d YMR320W hom 0.0234956175972035 0.173575242825424 608 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL061W ALD6 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0234933334157173 0.17361707664223 609 Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress response to salt stress|NADPH regeneration|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity cytosol|mitochondrion YGL124C MON1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0234906010904203 0.173667127913967 610 Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate autophagy|vesicle docking|protein targeting to vacuole molecular_function cytosol|fungal-type vacuole membrane|Mon1-Ccz1 complex YPL056C_p LCL1 hom FT PLASMA MEMBRANE 0.0234377816087585 0.174701087281668 611 Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan biological_process molecular_function cellular_component YHR106W TRR2 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0233961919476505 0.175403158886579 612 Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication cellular response to oxidative stress|cell redox homeostasis thioredoxin-disulfide reductase activity mitochondrion YJR103W URA8 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0233458529126852 0.176334085580355 613 Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YGR002C SWC4 het FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0233453756213411 0.176342929778812 614 Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex DNA repair|histone acetylation|chromosome organization|chromatin modification|chromatin remodeling|histone exchange|establishment of mitotic sister chromatid cohesion DNA binding Swr1 complex|histone acetyltransferase complex|nucleus|NuA4 histone acetyltransferase complex YER056C-A RPL34A hom FT TRANSLATION|RIBOSOME 0.0233356539806443 0.176523143508538 615 Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YAL002W VPS8 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0233166525821494 0.177069540340944 616 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif late endosome to vacuole transport via multivesicular body sorting pathway|protein targeting to vacuole GTPase binding|protein anchor late endosome|membrane|CORVET complex YOR112W CEX1 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.023278294713614 0.177653875998966 617 Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p tRNA export from nucleus tRNA binding nuclear pore|cytoplasm YDR223W CRF1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.023236841433991 0.178362659765926 618 Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter transcription corepressor activity nuclear chromatin|nucleus|cytoplasm YBL082C ALG3 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0232207243144169 0.178664047205087 619 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation alpha-1,3-mannosyltransferase activity integral to membrane|endoplasmic reticulum YML048W GSF2 hom FF|FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0232154800890388 0.178762194953295 620 ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression protein folding|protein localization to cell surface unfolded protein binding mitochondrial outer membrane|integral to endoplasmic reticulum membrane|cytoplasm YGR237C_p YGR237C hom 0.0231991624406809 0.179327314147142 621 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YJR119C JHD2 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0231610407515798 0.17978341776984 622 JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation histone demethylation histone demethylase activity (H3-K4 specific) nucleus|cytoplasm YLR329W REC102 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0231535025293503 0.180380244719937 623 Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome|nucleus YFR032C_p RRT5 hom 0.0231394960231253 0.180188768252192 624 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function cellular_component YFL024C EPL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0231238744930899 0.181722667685232 625 Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YPL184C MRN1 hom FT TRANSLATION 0.0231125676331908 0.180696362717848 626 RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation biological_process molecular_function cytoplasm YGL111W NSA1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0231117666554877 0.180711477216932 627 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YLR114C AVL9 hom FT VESICLE-MEDIATED TRANSPORT 0.0230870998705465 0.181177400548681 628 Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress post-Golgi vesicle-mediated transport molecular_function cytoplasm YJR075W HOC1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0230653154634014 0.181589620192562 629 Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele cell wall mannoprotein biosynthetic process|substituted mannan metabolic process alpha-1,6-mannosyltransferase activity|transferase activity, transferring glycosyl groups alpha-1,6-mannosyltransferase complex YNL114C_d YNL114C het 0.0230636281688792 0.181621577351919 630 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Unknown Unknown Unknown YML055W SPC2 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0230246092134572 0.182361755782308 631 Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex YDR420W HKR1 hom FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0230178357626003 0.182686247422081 632 Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection (1->3)-beta-D-glucan biosynthetic process|fungal-type cell wall organization|osmosensory signaling pathway via Sho1 osmosensor|hyperosmotic response|cellular bud site selection osmosensor activity site of polarized growth|plasma membrane YGL045W RIM8 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0229810299203251 0.183387099362062 633 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family meiosis|protein processing|invasive growth in response to glucose limitation|ubiquitin-dependent endocytosis molecular_function internal side of plasma membrane YJR033C RAV1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM 0.0229686901904943 0.183426418538282 634 Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate early endosome to late endosome transport|vacuolar acidification|regulation of protein complex assembly molecular_function RAVE complex|cytoplasm|extrinsic to membrane YPR071W_p YPR071W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0229150345152889 0.184452309169333 635 Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C biological_process molecular_function integral to membrane YDR436W PPZ2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0229050197282233 0.184644260017603 636 Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular sodium ion homeostasis protein serine/threonine phosphatase activity cellular_component YLR215C CDC123 het FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.0228981645116768 0.184841229897005 637 Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein regulation of mitotic cell cycle|regulation of translation molecular_function cytoplasm YCL045C EMC1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0228922124586845 0.184889948923944 638 Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YDL018C ERP3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.022885599392083 0.185016905638741 639 Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport vesicle-mediated transport molecular_function integral to membrane YAR020C PAU7 hom 0.022885076464177 0.185026947484279 640 Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component YNL043C_d YNL043C hom 0.0228293058537211 0.186100232075258 641 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Unknown Unknown Unknown YHR171W ATG7 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0227769610487372 0.187111766379301 642 Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation C-terminal protein lipidation|protein modification by small protein conjugation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway APG8 activating enzyme activity|APG12 activating enzyme activity cytosol|mitochondrion|membrane|pre-autophagosomal structure YEL003W GIM4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.022722431885814 0.188763616346405 643 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YLR071C RGR1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0227182241717123 0.188251649553928 644 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding core mediator complex YDL225W SHS1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0226690176510486 0.189540697074462 645 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress cytokinesis, actomyosin contractile ring assembly|G1 phase of mitotic cell cycle|barrier septum assembly|exit from mitosis|cell cycle cytokinesis|septin ring assembly|cellular bud neck septin ring organization GTP binding cellular bud neck septin ring|mating projection tip YKL007W CAP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0226330109242941 0.189914440606251 646 Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YKL020C SPT23 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0226288785222739 0.190193632439833 647 ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting cellular response to cold|positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA binding integral to endoplasmic reticulum membrane|nucleus YLR328W NMA1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0225940966758922 0.190677372789702 648 Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication NAD biosynthetic process nicotinamide-nucleotide adenylyltransferase activity nucleus|cytoplasm YDR415C_p YDR415C hom FT PROTEOLYSIS 0.022589659595433 0.190764506881518 649 Putative aminopeptidase biological_process molecular_function cellular_component YOR239W ABP140 hom FT RNA PROCESSING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0225867947866504 0.190820780723747 650 AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift actin filament bundle assembly|tRNA methylation tRNA (cytosine-3-)-methyltransferase activity|actin filament binding|protein binding, bridging actin filament|actin filament bundle|mating projection tip|actin cortical patch YCL008C STP22 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.0225610564481098 0.191326910437858 651 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT I complex|internal side of plasma membrane YHR124W NDT80 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.02255186304018 0.191507932649988 652 Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) meiosis|transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nuclear chromosome YPL198W RPL7B hom FT TRANSLATION|NUCLEUS|RIBOSOME 0.0224323006916707 0.19387364743635 653 Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|nucleolus|cytoplasm YOR149C SMP3 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0224129479250883 0.194258577913798 654 Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure GPI anchor biosynthetic process|plasmid maintenance dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to membrane|endoplasmic reticulum YDR406W PDR15 hom FT NUCLEOTIDE METABOLISM 0.0224025755173311 0.194465117848497 655 Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element transport|response to drug ATPase activity, coupled to transmembrane movement of substances integral to membrane YMR294W-A_d YMR294W-A hom 0.0223477253882784 0.19555999595813 656 Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Unknown Unknown Unknown YOL056W GPM3 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0223474174314449 0.195566155892161 657 Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process phosphoglycerate mutase activity|molecular_function cytosol YPL154C PEP4 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0223120935072506 0.19627366961475 658 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates microautophagy|proteolysis involved in cellular protein catabolic process|cellular response to starvation peptidase activity fungal-type vacuole lumen|mitochondrion|fungal-type vacuole YML107C PML39 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.022285428581895 0.196808989777332 659 Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes maintenance of RNA location molecular_function ribosome|nuclear pore YJL108C PRM10 hom 0.0222814319932648 0.196889316562401 660 Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function integral to membrane YGR263C SAY1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0222605238968143 0.197309936137785 661 Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum response to toxin|sterol metabolic process|sterol deacetylation steryl deacetylase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum lumen YMR313C TGL3 hom FT LIPID METABOLISM|CELL DIVISION 0.0222353726012452 0.197816789096688 662 Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation triglyceride catabolic process|cell budding|cellular lipid metabolic process triglyceride lipase activity|lysophosphatidylethanolamine acyltransferase activity lipid particle YOR069W VPS5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0222067791935589 0.198729055508989 663 Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes protein retention in Golgi apparatus|retrograde transport, endosome to Golgi protein transporter activity|phosphatidylinositol-3-phosphate binding cytosol|endosome|retromer complex, outer shell|retromer complex YER027C GAL83 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0221916244955481 0.198700675305921 664 One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain signal transduction|cell adhesion|protein phosphorylation|invasive growth in response to glucose limitation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|cytoplasm YLR183C TOS4 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0221564096519379 0.19941424938358 665 Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication response to DNA damage stimulus chromatin binding nuclear chromatin|Rpd3L-Expanded complex|mitochondrion|nucleus|cytoplasm YCL009C ILV6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0221540153552674 0.199462833942587 666 Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria branched-chain amino acid biosynthetic process enzyme regulator activity|acetolactate synthase activity mitochondrial nucleoid|acetolactate synthase complex|mitochondrion YKL148C SDH1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0221289592451222 0.199971785831279 667 Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits cellular respiration|mitochondrial electron transport, succinate to ubiquinone|tricarboxylic acid cycle succinate dehydrogenase (ubiquinone) activity|flavin adenine dinucleotide binding mitochondrial respiratory chain complex II|mitochondrion|mitochondrial membrane YOR336W KRE5 het FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM 0.0221165699896509 0.20022379235964 668 Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects (1->6)-beta-D-glucan biosynthetic process UDP-glucose:glycoprotein glucosyltransferase activity endoplasmic reticulum YNL052W COX5A hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0220955858185777 0.200651154330674 669 Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YAL037W_p YAL037W hom 0.0220425686825703 0.201733860753937 670 Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication biological_process molecular_function cellular_component YNL146C-A_p YNL146C-A hom 0.0220376373916165 0.201834782712804 671 Putative protein of unknown function biological_process molecular_function cellular_component YJL065C DLS1 hom FT GENE SILENCING|NUCLEUS 0.0220235888492596 0.202391780090871 672 Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication chromatin silencing at telomere molecular_function chromatin accessibility complex|nucleus YKL142W MRP8 hom 0.0219964418586612 0.202679312932783 673 Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis biological_process molecular_function cytoplasm YDR250C_d YDR250C hom 0.0219892431010592 0.202827155010988 674 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YDR015C_d YDR015C hom 0.0219498304615606 0.203637970825328 675 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Unknown Unknown Unknown YKL112W ABF1 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS 0.0219219434766179 0.204213098298952 676 DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair negative regulation of transcription from RNA polymerase II promoter|global genome nucleotide-excision repair|chromatin remodeling|DNA-dependent DNA replication|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific DNA binding, bending nucleotide-excision repair factor 4 complex|nucleus YLR377C FBP1 hom FT CARBOHYDRATE METABOLISM 0.0219162200352691 0.204331281693264 677 Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p gluconeogenesis|reactive oxygen species metabolic process fructose 1,6-bisphosphate 1-phosphatase activity cytosol YLR019W PSR2 hom FT PLASMA MEMBRANE 0.021906675130425 0.205001558915232 678 Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p response to stress phosphoprotein phosphatase activity plasma membrane YER051W JHD1 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.021815568087961 0.206417778301671 679 JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe histone demethylation methylated histone residue binding|histone demethylase activity (H3-K36 specific) cellular_component YPL111W CAR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS|SITE OF POLARIZED GROWTH 0.0218068658959147 0.206598896837049 680 Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance regulation of ornithine metabolic process|arginine catabolic process to ornithine manganese ion binding|arginase activity|ornithine carbamoyltransferase inhibitor activity|zinc ion binding cytosol|nucleus|mating projection tip|cytoplasm YER073W ALD5 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0218065297025073 0.206605896336283 681 Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity mitochondrion YNR033W ABZ1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0218006654409972 0.206998976117056 682 Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress para-aminobenzoic acid biosynthetic process 4-amino-4-deoxychorismate synthase activity cytoplasm YLR106C MDN1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|NUCLEUS|MITOCHONDRION 0.021796861841913 0.206807253464158 683 Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus ribosomal large subunit assembly|rRNA processing ATPase activity nucleoplasm|mitochondrion|nucleus YDR165W TRM82 hom FT RNA PROCESSING|NUCLEUS 0.0217894242124148 0.206962257387065 684 Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YML001W YPT7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|MITOCHONDRION 0.0217687858336817 0.207731870239924 685 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole inheritance|Golgi to vacuole transport|vesicle-mediated transport|endocytosis GTPase activity mitochondrial outer membrane|fungal-type vacuole YNL001W DOM34 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.0217627464735347 0.207722317162599 686 Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication nuclear-transcribed mRNA catabolic process, no-go decay|ribosome disassembly|nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay|nonfunctional rRNA decay ribosome binding|endoribonuclease activity cytoplasm YNL113W RPC19 het FT NUCLEUS 0.0217617311634731 0.207540135514162 687 RNA polymerase subunit AC19, common to RNA polymerases I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex YIR004W DJP1 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0217561468685075 0.207656806078817 688 Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ protein import into peroxisome matrix chaperone binding cytosol YNL067W-B_p YNL067W-B hom 0.0216328952756572 0.210243972977478 689 Putative protein of unknown function biological_process molecular_function cellular_component YGR110W CLD1 hom FT LIPID METABOLISM|MITOCHONDRION 0.0216071271252584 0.210923934112177 690 Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 cardiolipin acyl-chain remodeling|cardiolipin metabolic process phospholipase A2 activity mitochondrion YGR173W RBG2 hom FT TRANSLATION 0.0216026081807206 0.210883281349631 691 Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein cytoplasmic translation GTP binding cytoplasm YPL131W RPL5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0215984134593566 0.211653778540932 692 Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YJR091C JSN1 hom FT PROTEIN LOCALIZATION|MITOCHONDRION 0.0215706020703208 0.21176482843701 693 Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|cellular protein complex localization|establishment of mitochondrion localization mRNA binding external side of mitochondrial outer membrane YLR410W VIP1 hom FT CYTOSKELETON 0.0215550368294051 0.211890266076034 694 Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 inositol phosphate biosynthetic process inositol hexakisphosphate 1-kinase activity|inositol hexakisphosphate 3-kinase activity|inositol heptakisphosphate kinase activity|inositol hexakisphosphate 4-kinase activity|inositol hexakisphosphate 6-kinase activity cytoplasm YGL063W PUS2 hom FT RNA PROCESSING|MITOCHONDRION 0.0215399713190321 0.212209894018393 695 Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion YDR100W TVP15 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0215378174342993 0.212255618993745 696 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p vesicle-mediated transport molecular_function COPI-coated vesicle|integral to membrane|integral to Golgi membrane YLL050C COF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0214904343242583 0.213331576072924 697 Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes actin filament depolymerization|actin filament severing actin filament binding plasma membrane|actin cortical patch YML024W RPS17A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0214818314952289 0.213446645422123 698 Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YOR053W_d YOR053W hom 0.0214717007127308 0.214141252352361 699 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Unknown Unknown Unknown YNR055C HOL1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.021465519601019 0.213862865056073 700 Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake organic alcohol transport|cation transport alcohol transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YOR233W KIN4 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0214561665420497 0.214336085064295 701 Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication mitotic cell cycle spindle orientation checkpoint|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity cell cortex|spindle pole body|cellular bud neck YER007C-A TMA20 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION 0.0214519746721051 0.214083777145244 702 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress ribosome biogenesis RNA binding ribosome|cytoplasm YDR304C CPR5 hom FT ENDOPLASMIC RETICULUM 0.0214500567336203 0.214124751942446 703 Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER biological_process peptidyl-prolyl cis-trans isomerase activity endoplasmic reticulum|cytoplasm YOR017W PET127 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.021440169634847 0.214336069408791 704 Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane mitochondrial RNA 5'-end processing molecular_function mitochondrial envelope|mitochondrion YDR486C VPS60 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0214360346339673 0.214424491558131 705 Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p late endosome to vacuole transport via multivesicular body sorting pathway|filamentous growth molecular_function fungal-type vacuole membrane|cytoplasm YMR194C-A_d YMR194C-A hom 0.0213865382958534 0.21548495414436 706 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR283C YGR283C hom FT NUCLEUS 0.0213713020170407 0.215812151408745 707 Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus biological_process S-adenosylmethionine-dependent methyltransferase activity ribosome|nucleolus YLL055W YCT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0213574099557792 0.216110793327203 708 High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene cysteine transport ion transmembrane transporter activity|cysteine transmembrane transporter activity integral to membrane|endoplasmic reticulum YOR007C SGT2 hom FT PROTEIN LOCALIZATION 0.0213543441570839 0.216176739785997 709 Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress response to heat|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YKL086W SRX1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0213511782451839 0.216244854913772 710 Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress cellular response to oxidative stress sulfiredoxin activity nucleus|cytoplasm YMR193C-A_d YMR193C-A hom 0.0213239860717509 0.216830535156397 711 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YAL013W DEP1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0213105618522389 0.21712009376785 712 Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at rDNA|regulation of transcription from RNA polymerase II promoter|histone deacetylation|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of chromatin silencing at telomere|negative regulation of transcription from RNA polymerase II promoter|regulation of DNA-dependent DNA replication initiation|Unknown molecular_function Rpd3L-Expanded complex|Rpd3L complex YIL093C RSM25 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0213067199377563 0.217203014495362 713 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YPL067C_p YPL067C hom 0.0212565846894893 0.218287180282339 714 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm YNR009W NRM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0212507505535704 0.218413594466236 715 Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase negative regulation of transcription involved in G1 phase of mitotic cell cycle repressing transcription factor binding|transcription factor binding transcription factor activity MBF transcription complex|nucleus|cytoplasm YER137C_p YER137C hom 0.0212484824539217 0.218462753889577 716 Putative protein of unknown function biological_process molecular_function cellular_component YER060W FCY21 hom 0.021181826567011 0.219911022000989 717 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YAL042W ERV46 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0211706396224089 0.22015476003281 718 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YNR061C YNR061C hom 0.0211653933931734 0.220269130115017 719 Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress biological_process molecular_function integral to membrane|fungal-type vacuole YLR390W ECM19 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0211584794385339 0.220557583113236 720 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies fungal-type cell wall organization molecular_function mitochondrion YGR197C SNG1 hom FT PLASMA MEMBRANE 0.021151559132703 0.220570927293767 721 Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance nucleobase-containing compound transport molecular_function integral to membrane|plasma membrane YMR083W ADH3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0211146227746308 0.221378153257238 722 Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production NADH oxidation|fermentation|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity mitochondrion|mitochondrial matrix YOR184W SER1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0210887680980193 0.22242750327882 723 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress serine family amino acid biosynthetic process|L-serine biosynthetic process|purine nucleobase biosynthetic process O-phospho-L-serine:2-oxoglutarate aminotransferase activity cytoplasm YAL048C GEM1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.021084618006186 0.222035448770536 724 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion inheritance|regulation of mitochondrion organization|phospholipid homeostasis GTPase activity|calcium ion binding mitochondrial outer membrane|ERMES complex|mitochondrion YDR077W SED1 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0210528726500435 0.222732394044049 725 Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites fungal-type cell wall organization|mitochondrial genome maintenance structural constituent of cell wall mitochondrion|ribosome|fungal-type cell wall YIR036C_p IRC24 hom FT OXIDATION-REDUCTION PROCESS 0.0210243063422544 0.223360882397689 726 Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci biological_process molecular_function integral to membrane|cytoplasm YNR067C DSE4 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION 0.0210087768293645 0.223703079357284 727 Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother cytokinesis, completion of separation glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall|extracellular region|cell septum YER091C-A_d YER091C-A hom 0.0210080834038657 0.223718367908947 728 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YAL022C FUN26 hom 0.0209480467171994 0.225044881653029 729 Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis nucleoside transport|nicotinamide riboside transport|transmembrane transport nicotinamide riboside transporter activity|nucleoside transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|intracellular|membrane YBR259W YBR259W hom 0.0209339004491628 0.225358260048224 730 Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component YER010C YER010C hom 0.0209193780760742 0.225680294089723 731 Protein of unknown function, forms a ring-shaped homotrimer; has similarity to members of the prokaryotic rraA family; possibly involved in a phosphotransfer reaction biological_process molecular_function cellular_component YKL167C MRP49 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0209157704675414 0.225760343808394 732 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YBR016W YBR016W hom FT PLASMA MEMBRANE 0.020891790810786 0.226292946621408 733 Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication biological_process molecular_function cellular bud membrane|plasma membrane|mating projection membrane YDR035W ARO3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS|MITOCHONDRION 0.0208830472767747 0.226903271634974 734 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity mitochondrion|nucleus|cytoplasm YEL010W_d YEL010W hom 0.0208639087925664 0.226913349288834 735 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR316C-A_p YOR316C-A hom 0.0208426971909368 0.227386139897064 736 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YKR045C_p YKR045C hom 0.0208138734946011 0.228724454192623 737 Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm YHR115C DMA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0207887618395912 0.228591478469904 738 Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger establishment of mitotic spindle orientation|protein autoubiquitination|septin ring assembly|mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity cytoplasm YLR292C SEC72 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0207558822394425 0.229328493892908 739 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation protein transporter activity Sec62/Sec63 complex|endoplasmic reticulum membrane YKL041W VPS24 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.0207336577495728 0.229827625184968 740 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YML120C NDI1 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0207245811444572 0.230031695148991 741 NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID mitochondrial electron transport, NADH to ubiquinone|NADH oxidation|chronological cell aging|positive regulation of apoptotic process NADH dehydrogenase (ubiquinone) activity mitochondrion|mitochondrial matrix YCR030C SYP1 hom FT CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0207203199028737 0.230127545365917 742 Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization septin cytoskeleton organization|actin cortical patch assembly molecular_function mating projection base|mating projection tip|cellular bud neck|cellular bud tip YJL115W ASF1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0206854010114401 0.23209985247446 743 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition DNA replication-independent nucleosome assembly|histone acetylation|nucleosome disassembly|histone exchange|positive regulation of histone acetylation|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere|DNA replication-dependent nucleosome assembly|regulation of transcription from RNA polymerase II promoter in response to stress histone binding chromatin assembly complex YNL236W SIN4 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0206633608924167 0.2320386259266 744 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding core mediator complex YDL191W RPL35A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0206524139169573 0.231658820287861 745 Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YOR165W SEY1 hom FT MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0206348348299288 0.232056403185128 746 Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 cellular membrane organization|endoplasmic reticulum organization|endoplasmic reticulum membrane fusion|endoplasmic reticulum inheritance GTPase activity integral to membrane|endoplasmic reticulum|cortical endoplasmic reticulum|cytoplasm YLR417W VPS36 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0206152176150831 0.232709683569247 747 Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome negative regulation of transcription from RNA polymerase II promoter by glucose|protein retention in Golgi apparatus|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole ubiquitin binding ESCRT II complex YDL079C MRK1 hom FT PROTEIN PHOSPHORYLATION 0.0205516854563545 0.233943537770462 748 Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation regulation of protein catabolic process|protein phosphorylation|response to stress protein serine/threonine kinase activity cellular_component YHL024W RIM4 hom FT CELL CYCLE 0.0205323311301513 0.234384353400548 749 Putative RNA-binding protein required for the expression of early and middle sporulation genes meiosis|reciprocal meiotic recombination|sporulation resulting in formation of a cellular spore|premeiotic DNA replication RNA binding cytoplasm YKL065C YET1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0205153533032328 0.234771525682072 750 Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function ribosome|integral to membrane|endoplasmic reticulum|endoplasmic reticulum membrane YML078W CPR3 hom FT MITOCHONDRION 0.02048888216104 0.235376091869329 751 Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria protein folding|apoptotic process peptidyl-prolyl cis-trans isomerase activity mitochondrion YEL030W ECM10 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.020481953181361 0.235604362174079 752 Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication protein refolding|protein targeting to mitochondrion molecular_function mitochondrial nucleoid|mitochondrion YDL167C NRP1 hom 0.0204413839122421 0.236463649108866 753 Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis biological_process molecular_function cytoplasmic stress granule|cytoplasm YLL006W MMM1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0204156924500274 0.237193265676483 754 ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization|mitochondrial genome maintenance|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrion|integral to endoplasmic reticulum membrane|ERMES complex YMR115W MGR3 hom FT PROTEOLYSIS|MITOCHONDRION 0.0204142472362651 0.237086583571882 755 Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA misfolded or incompletely synthesized protein catabolic process misfolded protein binding mitochondrion|mitochondrial inner membrane|i-AAA complex YMR170C ALD2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0204077318227798 0.237236320199823 756 Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular aldehyde metabolic process|beta-alanine biosynthetic process|polyamine catabolic process aldehyde dehydrogenase (NAD) activity cytoplasm YML067C ERV41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0203736329567447 0.23802106667705 757 Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YCR020W-B HTL1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|RSC COMPLEX 0.0203699992145769 0.238735635978437 758 Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance regulation of cell cycle|nucleosome disassembly|response to DNA damage stimulus|chromatin remodeling|transcription elongation from RNA polymerase II promoter chromatin binding RSC complex YDR233C RTN1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0203475357979927 0.238622899389352 759 Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum organization|endoplasmic reticulum tubular network maintenance|nuclear pore complex assembly molecular_function integral to endoplasmic reticulum membrane|mitochondrion|Golgi apparatus|endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YDR313C PIB1 hom 0.0202907782711797 0.240005600174768 760 RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain protein ubiquitination ubiquitin-protein ligase activity|phosphatidylinositol-3-phosphate binding fungal-type vacuole membrane|late endosome YGL177W_d YGL177W hom 0.0202889005942536 0.239979012804782 761 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL090C DPB11 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.0202428597254104 0.241047643804967 762 DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress lagging strand elongation|recombinational repair|nucleotide-excision repair|DNA replication initiation|mismatch repair|positive regulation of protein phosphorylation|mating type switching|DNA replication checkpoint|double-strand break repair via break-induced replication|mitotic cell cycle G2/M transition DNA damage checkpoint|leading strand elongation protein kinase activator activity|protein binding DNA replication preinitiation complex|replication fork|epsilon DNA polymerase complex YDR270W CCC2 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0202392794020801 0.241130884994277 763 Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism cellular iron ion homeostasis|copper ion export|transmembrane transport copper ion binding|cation-transporting ATPase activity integral to membrane|trans-Golgi network transport vesicle membrane YJL110C GZF3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0202290143359453 0.241369656124405 764 GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication maintenance of protein location in nucleus|nitrogen catabolite repression of transcription|negative regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding nucleus YDR325W YCG1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0202148972361826 0.241698299352653 765 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation rDNA condensation|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation molecular_function nuclear condensin complex YOR178C GAC1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.020205408843633 0.242130065895755 766 Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock meiosis|glycogen metabolic process|negative regulation of transcription from RNA polymerase II promoter|response to heat|mitotic cell cycle spindle assembly checkpoint protein phosphatase type 1 regulator activity|structural molecule activity|heat shock protein binding protein phosphatase type 1 complex YHL023C NPR3 hom FT CELL CYCLE|SIGNALING|RESPONSE TO NUTRIENT LEVELS 0.0201930471243359 0.242207585966073 767 Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair meiosis|regulation of TOR signaling cascade|cellular response to amino acid starvation molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YKL034W TUL1 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0201604694287232 0.242968313567398 768 Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|Golgi apparatus YGR065C VHT1 het FT PLASMA MEMBRANE 0.0201455077974786 0.243599537077124 769 High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin biotin transport biotin transporter activity integral to membrane|plasma membrane YCL043C PDI1 het FT ENDOPLASMIC RETICULUM 0.0201240479178692 0.243820784653843 770 Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication protein folding protein disulfide oxidoreductase activity|protein disulfide isomerase activity endoplasmic reticulum lumen YPL235W RVB2 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0201070584403902 0.244782513172563 771 ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly regulation of transcription from RNA polymerase II promoter|rRNA processing|chromatin remodeling|box C/D snoRNP assembly|histone exchange ATP-dependent 5'-3' DNA helicase activity|ATP-dependent 3'-5' DNA helicase activity nuclear telomeric heterochromatin|ASTRA complex|R2TP complex|Swr1 complex|nucleus|Ino80 complex YOL100W PKH2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0200974023179713 0.244445772357395 772 Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p regulation of nuclear-transcribed mRNA poly(A) tail shortening|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cell cortex|eisosome|nucleus YOL061W PRS5 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0200909174768149 0.244598047937679 773 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YGL153W PEX14 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0200553292249157 0.245434908097953 774 Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p protein import into peroxisome matrix, docking protein binding, bridging peroxisomal membrane YCR107W AAD3 hom FT OXIDATION-REDUCTION PROCESS 0.0200355527469529 0.245900820056461 775 Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YOL139C CDC33 het FT CELL CYCLE|TRANSLATION|NUCLEUS|RIBOSOME 0.0200041212154279 0.246642586944209 776 mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth regulation of cell cycle|translational initiation|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay phosphatidylinositol-3-phosphate binding|translation initiation factor activity ribosome|nucleus|eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule|cytoplasm YPR189W SKI3 hom FT NUCLEUS 0.0200012238408951 0.246711042191585 777 Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function Ski complex YOR076C SKI7 hom FT TRANSLATION|NUCLEOTIDE METABOLISM 0.0199912626222143 0.246946493897523 778 Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay|nonfunctional rRNA decay protein binding, bridging Ski complex|cytoplasmic exosome (RNase complex)|cytoplasm YKR103W NFT1 hom FT NUCLEOTIDE METABOLISM 0.0199537231822477 0.247905740770657 779 Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YER066W_p RRT13 hom 0.0199344954749181 0.248291292649298 780 Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component YNR004W_p SWM2 hom FT NUCLEUS 0.0199069904245911 0.248944718534148 781 Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds biological_process molecular_function nucleolus YOL064C MET22 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0198504333399975 0.250292095252083 782 Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway methionine biosynthetic process|hyperosmotic salinity response|sulfate assimilation 3'(2'),5'-bisphosphate nucleotidase activity cellular_component YGL236C MTO1 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0198482243016382 0.250344824943354 783 Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants mitochondrial tRNA wobble uridine modification molecular_function mitochondrion YDR105C TMS1 hom 0.0198230455248414 0.250946389619447 784 Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function fungal-type vacuole membrane|integral to membrane|membrane YPR110C RPC40 het FT NUCLEUS 0.0198031068811833 0.251423473334739 785 RNA polymerase subunit AC40, common to RNA polymerase I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex YLR073C RFU1 hom 0.0197944945123056 0.251629742047822 786 Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes regulation of ubiquitin homeostasis enzyme inhibitor activity endosome YHL036W MUP3 hom 0.0197719809473423 0.252523392742224 787 Low affinity methionine permease, similar to Mup1p amino acid transport L-methionine transmembrane transporter activity integral to membrane|membrane YLR249W YEF3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0197718310088217 0.252173103105739 788 Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication translational elongation|translational termination ATPase activity|translation elongation factor activity cytosolic ribosome|ribosome YAR050W FLO1 hom FT PLASMA MEMBRANE 0.0197483205547983 0.252737633388506 789 Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p cellular response to stress|flocculation via cell wall protein-carbohydrate interaction|flocculation mannose binding fungal-type cell wall YDL125C HNT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0197410910080577 0.25291140523633 790 Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress nucleotide metabolic process hydrolase activity|nucleotide binding nucleus|cytoplasm YDR180W SCC2 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0197370169515787 0.253080134597503 791 Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin double-strand break repair|establishment of protein localization to chromatin|protein localization to chromatin|2-micrometer plasmid partitioning|mitotic chromosome condensation|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery|establishment of mitotic sister chromatid cohesion|rDNA condensation|tRNA gene clustering molecular_function nuclear chromatin|2-micrometer circle DNA|SMC loading complex YGR121C MEP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0197287763981686 0.253207594923469 792 Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YKL008C LAC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0197156698156266 0.253523098242259 793 Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p replicative cell aging|ceramide biosynthetic process sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YDR344C_d YDR344C hom 0.0196946260116357 0.254030239493072 794 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR178W_d YBR178W hom 0.0196815550851825 0.254345594941591 795 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Unknown Unknown Unknown YDR008C_d YDR008C hom FF 0.0196701773873321 0.255614291611637 796 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR451W LEU3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0196417390395884 0.255378763596376 797 Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YKL141W SDH3 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0196020606662111 0.256269378369926 798 Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex mitochondrial electron transport, succinate to ubiquinone|cellular respiration|tricarboxylic acid cycle|protein import into mitochondrial inner membrane succinate dehydrogenase (ubiquinone) activity mitochondrial respiratory chain complex II|mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YOR299W BUD7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0195830039105175 0.257015925670539 799 Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process|cellular bud site selection molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YMR268C PRP24 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0195506426792728 0.257519072631307 800 Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling assembly of spliceosomal tri-snRNP|spliceosomal complex assembly U6 snRNA binding|snRNA binding U6 snRNP YIL110W HPM1 hom FT NUCLEUS 0.019532867087535 0.258023066102438 801 AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance peptidyl-histidine methylation, to form tele-methylhistidine S-adenosylmethionine-dependent methyltransferase activity|protein-histidine N-methyltransferase activity nucleus|cytoplasm YOR137C SIA1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0194612689284082 0.259985674578204 802 Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose proton transport molecular_function cellular_component YDR217C RAD9 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0194388941465488 0.260249632660275 803 DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate DNA repair|mitotic cell cycle G1/S transition DNA damage checkpoint|regulation of cell cycle|intra-S DNA damage checkpoint|nucleotide-excision repair|DNA damage checkpoint|positive regulation of transcription from RNA polymerase II promoter double-stranded DNA binding|histone binding chromatin|nucleus YDR245W MNN10 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0194325831077709 0.260617758453074 804 Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family N-glycan processing|cell wall mannoprotein biosynthetic process|substituted mannan metabolic process|barrier septum assembly alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YDR329C PEX3 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOPLASMIC RETICULUM 0.019411958839734 0.261053007836243 805 Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization protein binding peroxisomal membrane|endoplasmic reticulum YMR318C ADH6 hom FT OXIDATION-REDUCTION PROCESS 0.0194035159659802 0.26111825252181 806 NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress cellular aldehyde metabolic process|furaldehyde metabolic process|alcohol metabolic process hydroxymethylfurfural reductase (NADH) activity|hydroxymethylfurfural reductase (NADPH) activity|alcohol dehydrogenase (NADP+) activity cellular_component YFL052W_p YFL052W hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0193669740598612 0.262017546286285 807 Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity biological_process DNA binding cellular_component YKL191W DPH2 hom 0.0193428610220706 0.262612136555255 808 Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YDR283C GCN2 hom FF|FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|RIBOSOME 0.0193156049100795 0.263499005099714 809 Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control cellular amino acid biosynthetic process|protein phosphorylation|regulation of translational initiation|DNA damage checkpoint protein homodimerization activity|protein kinase activity|eukaryotic translation initiation factor 2alpha kinase activity cytosolic ribosome YML018C YML018C hom 0.0192702657649973 0.264764260869361 810 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication biological_process molecular_function fungal-type vacuole membrane|integral to membrane YMR013W-A_d YMR013W-A hom 0.0192539681672076 0.26481213884546 811 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Unknown Unknown Unknown YJL033W HCA4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0192410920687527 0.265345715638487 812 DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis rRNA processing RNA-dependent ATPase activity|ATP-dependent RNA helicase activity nucleolus YER016W BIM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0192154488425939 0.265769382064484 813 Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally nuclear migration along microtubule|microtubule nucleation|microtubule depolymerization|negative regulation of microtubule depolymerization|positive regulation of microtubule polymerization|mitotic sister chromatid cohesion|mitotic cell cycle spindle assembly checkpoint microtubule plus-end binding|protein homodimerization activity|structural constituent of cytoskeleton spindle midzone|microtubule plus end|cytoplasmic microtubule|spindle pole body|kinetochore|spindle pole|spindle YJL121C RPE1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.0192079044974913 0.265957145026333 814 D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress pentose-phosphate shunt ribulose-phosphate 3-epimerase activity cytosol YOR192C THI72 hom 0.0191964830991791 0.266526922327345 815 Transporter of thiamine or related compound; shares sequence similarity with Thi7p thiamine transport transporter activity integral to membrane|membrane YGR167W CLC1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0191782126423739 0.26691102336898 816 Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W endocytosis|vesicle-mediated transport structural molecule activity clathrin vesicle coat YDR481C PHO8 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0191479277446367 0.267453088200854 817 Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN nicotinamide nucleotide metabolic process|protein dephosphorylation nucleotide phosphatase activity|alkaline phosphatase activity fungal-type vacuole membrane YKR094C RPL40B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0191362373049238 0.268102348853746 818 Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication cytoplasmic translation|protein ubiquitination|ribosome biogenesis structural constituent of ribosome|protein tag mitochondrion|cytosolic large ribosomal subunit YER034W YER034W hom FT NUCLEUS 0.0191290456135784 0.267925241671255 819 Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YLR012C_p YLR012C hom 0.0190955919949776 0.269120340442085 820 Putative protein of unknown function; YLR012C is not an essential gene biological_process molecular_function cellular_component YDR080W VPS41 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING 0.019000601310215 0.271152222346717 821 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole organization|vesicle-mediated transport|protein transport|vacuole fusion, non-autophagic|vacuolar protein processing Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding endosome|fungal-type vacuole membrane|HOPS complex YPL245W_p YPL245W hom FT NUCLEUS 0.0189866762378182 0.271503662539454 822 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus|cytoplasm YER093C-A AIM11 hom 0.018961685082182 0.272135169654485 823 Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication biological_process molecular_function cellular_component YOL107W_p YOL107W hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0189483546093543 0.272472431052343 824 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein biological_process molecular_function COPI-coated vesicle|Golgi apparatus YBR131W CCZ1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0189457562097055 0.272538203928117 825 Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway autophagy|autophagic vacuole fusion|vacuolar transport|vesicle docking|protein targeting to vacuole guanyl-nucleotide exchange factor activity Mon1-Ccz1 complex|membrane YOR025W HST3 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0189411955229671 0.272653674118785 826 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|histone deacetylation|chromatin silencing at telomere histone deacetylase activity|DNA binding nucleus YNL058C_p YNL058C hom 0.0189362227818589 0.272779615070931 827 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene biological_process molecular_function fungal-type vacuole YNL145W MFA2 hom FT SIGNALING|PLASMA MEMBRANE 0.0189353098405359 0.273375526135866 828 Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 pheromone-dependent signal transduction involved in conjugation with cellular fusion mating pheromone activity extracellular region YGR166W TRS65 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|GOLGI APPARATUS 0.0189287110752158 0.272969933951439 829 Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response intra-Golgi vesicle-mediated transport|protein complex assembly Rab guanyl-nucleotide exchange factor activity TRAPP complex|trans-Golgi network YKR078W YKR078W hom 0.0189191241406737 0.274432142495799 830 Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) biological_process phosphatidylinositol-3-phosphate binding cytoplasm YNL224C SQS1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|NUCLEUS 0.0189114558374801 0.273407461615188 831 Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress positive regulation of helicase activity|positive regulation of ATPase activity|mRNA splicing, via spliceosome|maturation of SSU-rRNA|rRNA processing molecular_function preribosome, small subunit precursor|nucleus|preribosome, large subunit precursor|90S preribosome|cytoplasm YGL251C HFM1 hom FT CELL CYCLE|NUCLEUS 0.0188913571725492 0.27391769178064 832 Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity meiosis|synapsis|DNA unwinding involved in replication|reciprocal meiotic recombination DNA helicase activity nucleus YDL096C_d OPI6 hom 0.0188580404725722 0.275051345612128 833 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Unknown Unknown Unknown YKL084W HOT13 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0188571161220298 0.274788441494825 834 Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein import into mitochondrial intermembrane space zinc ion binding mitochondrial intermembrane space YMR038C CCS1 hom FF|FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0188329394280377 0.276552673051431 835 Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress intracellular copper ion transport superoxide dismutase copper chaperone activity cytosol|mitochondrial inner membrane|nucleus YJL068C YJL068C hom 0.0188204619086498 0.275722649459601 836 Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D formaldehyde catabolic process S-formylglutathione hydrolase activity cytosol YOR005C DNL4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.0188149263443783 0.27586392271269 837 DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth double-strand break repair via nonhomologous end joining|replicative cell aging DNA ligase (ATP) activity DNA ligase IV complex|nuclear chromatin YPR013C_p CMR3 hom 0.0187913683020127 0.27675232574516 838 Putative zinc finger protein; YPR013C is not an essential gene biological_process sequence-specific DNA binding cellular_component YLR341W SPO77 hom FT CELL WALL ORG/BIOGENESIS 0.0187877444451487 0.276558348673361 839 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function intracellular YHR158C KEL1 hom FT CELL CYCLE|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH 0.0187469678056638 0.277602316456557 840 Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate negative regulation of exit from mitosis|regulation of cell shape|cell morphogenesis|cytogamy|plasma membrane fusion molecular_function mating projection tip|cellular bud neck|cytoplasm|cellular bud tip YLR086W SMC4 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0187408467620299 0.27775925932498 841 Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate mitotic chromosome condensation|mitotic sister chromatid segregation|tRNA gene clustering chromatin binding|ATPase activity SMC loading complex|nuclear condensin complex YGL226C-A OST5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0187202300567027 0.278288313525089 842 Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YOR217W RFC1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.0187147163194314 0.278429920034619 843 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon DNA repair|mismatch repair|mitotic cell cycle|leading strand elongation DNA clamp loader activity DNA replication factor C complex YDL105W NSE4 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0187098981995317 0.278840549786346 844 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair molecular_function Smc5-Smc6 complex|nucleus YPR176C BET2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION 0.0187072827121466 0.278620911161002 845 Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YBL042C FUI1 hom FF|FT PLASMA MEMBRANE 0.0187035679591556 0.278716387417048 846 High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress uridine transport|transmembrane transport uridine transmembrane transporter activity integral to membrane|plasma membrane YDR345C HXT3 hom FT PLASMA MEMBRANE 0.018692506696937 0.279000814756129 847 Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication hexose transport|transmembrane transport mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YBR031W RPL4A hom FT TRANSLATION|RIBOSOME 0.0186797058603443 0.279330219443482 848 Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOL019W_p YOL019W hom FT PLASMA MEMBRANE 0.0186597496253403 0.279844281475456 849 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication biological_process molecular_function integral to membrane|plasma membrane|fungal-type vacuole YKL222C YKL222C hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|CYTOSKELETON 0.0186576985393448 0.279897152760234 850 Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine biological_process sequence-specific DNA binding ribosome|nucleus|mitotic spindle YDR200C VPS64 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0186427907382429 0.280281638235089 851 Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance re-entry into mitotic cell cycle after pheromone arrest|protein targeting to vacuole molecular_function endoplasmic reticulum membrane|cytoplasm YER039C-A_p YER039C-A hom 0.018569041333362 0.282188975243074 852 Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function integral to membrane YHR175W-A_p YHR175W-A hom 0.0185664902829703 0.282255108592892 853 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YNL214W PEX17 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0185624882689422 0.282358877843017 854 Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis protein import into peroxisome matrix, docking protein binding peroxisomal membrane YBR036C CSG2 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0185426605593212 0.282873376962859 855 Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress cellular calcium ion homeostasis|glycosphingolipid biosynthetic process enzyme regulator activity integral to endoplasmic reticulum membrane|integral to membrane YPR103W PRE2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0185377758129911 0.283000226188014 856 Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasome core complex assembly|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YOR082C_d YOR082C hom 0.0185314283098944 0.283165118471933 857 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Unknown Unknown Unknown YPL258C THI21 hom 0.0185142257572057 0.283612324893563 858 Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p thiamine biosynthetic process phosphomethylpyrimidine kinase activity|hydroxymethylpyrimidine kinase activity cellular_component YHR095W_d YHR095W hom 0.0185046347026111 0.283861866363906 859 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNR001C CIT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0184295941394046 0.285819411539679 860 Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication glutamate biosynthetic process|tricarboxylic acid cycle|acetyl-CoA catabolic process|citrate metabolic process citrate (Si)-synthase activity mitochondrion YPL149W ATG5 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0183879378168554 0.286910005961174 861 Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation C-terminal protein lipidation|mitochondrion degradation|piecemeal microautophagy of nucleus|macroautophagy|CVT pathway Atg8 ligase activity cytosol|Atg12-Atg5-Atg16 complex|pre-autophagosomal structure|autophagic vacuole YDR078C SHU2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0183846514712078 0.287715900542886 862 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function recombinational repair|maintenance of rDNA|error-free translesion synthesis molecular_function Shu complex YBL097W BRN1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0183725429176319 0.287313765965733 863 Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation molecular_function nucleus|SMC loading complex|nuclear condensin complex YKL029C MAE1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0183596965537157 0.288010624915714 864 Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids pyruvate metabolic process|cellular amino acid metabolic process malic enzyme activity mitochondrion YLR350W ORM2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0183555431807899 0.287760060937901 865 Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress cellular sphingolipid homeostasis|negative regulation of sphingolipid biosynthetic process|response to unfolded protein molecular_function integral to membrane|endoplasmic reticulum|SPOTS complex YMR027W_p YMR027W hom FT NUCLEUS 0.0183505036593163 0.28789245353402 866 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene biological_process molecular_function nucleus|cytoplasm YBR014C GRX7 hom FT RESPONSE TO OXIDATIVE STRESS|GOLGI APPARATUS 0.01830236356349 0.289159205257594 867 Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication cellular response to oxidative stress glutathione-disulfide reductase activity|protein homodimerization activity integral to membrane|Golgi apparatus|Golgi lumen|fungal-type vacuole|cis-Golgi network YOL006C TOP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.018295567323583 0.289338342445091 868 Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination nuclear migration|chromatin assembly or disassembly|DNA topological change|DNA strand elongation involved in DNA replication|regulation of mitotic recombination|chromatin silencing at rDNA|regulation of transcription from RNA polymerase II promoter|mitotic chromosome condensation|transcription elongation from RNA polymerase II promoter DNA topoisomerase type I activity replication fork protection complex|nucleolus|nucleus YHR191C CTF8 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0182681577204742 0.290277423792758 869 Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion maintenance of DNA trinucleotide repeats|mitotic sister chromatid cohesion molecular_function Ctf18 RFC-like complex YIL020C HIS6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0182629872212393 0.290198135365287 870 Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts histidine biosynthetic process 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity intracellular YEL024W RIP1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0182100364133074 0.292031246236186 871 Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|mitochondrial respiratory chain complex III YDR516C EMI2 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0181972759132785 0.292441688590803 872 Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cytoplasm YPL163C SVS1 hom 0.0181700965997197 0.292658958652585 873 Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication response to chemical stimulus molecular_function fungal-type cell wall|fungal-type vacuole YPL226W NEW1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0181510788211196 0.293164492619286 874 ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction ribosomal small subunit biogenesis|poly(A)+ mRNA export from nucleus ATPase activity|ATP binding mitochondrion|polysome|cytoplasm YHR034C PIH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0181457054832861 0.293451421045162 875 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|rRNA processing|regulation of cell size|box C/D snoRNP assembly molecular_function R2TP complex|nucleus|small nucleolar ribonucleoprotein complex|cytoplasm YNL300W_p TOS6 hom 0.0181442506882014 0.293346142099082 876 Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid biological_process molecular_function fungal-type cell wall YKL039W PTM1 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0181384435468718 0.293500689432543 877 Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YPR075C OPY2 hom FT CELL CYCLE|SIGNALING|PLASMA MEMBRANE 0.0181366089577811 0.293549525294357 878 Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress cell cycle arrest in response to pheromone|osmosensory signaling pathway molecular_function integral to membrane|plasma membrane|fungal-type vacuole|cytoplasm YDR020C_p DAS2 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0181341780720769 0.293614242672807 879 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases biological_process molecular_function nucleus|cytoplasm YDL051W LHP1 hom FT RNA PROCESSING|NUCLEUS 0.0180987225669624 0.294559260667235 880 RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding|tRNA binding nucleoplasm|nucleolus|nucleus YMR283C RIT1 hom FT RNA PROCESSING 0.0180763900734839 0.295443722387968 881 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA charged-tRNA amino acid modification transferase activity, transferring pentosyl groups cytoplasm YGL117W_p YGL117W hom FF 0.018068146046987 0.295808286693974 882 Putative protein of unknown function biological_process molecular_function cellular_component YNL137C NAM9 het FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0180586492148337 0.295629811587255 883 Mitochondrial ribosomal component of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YKR002W PAP1 het FT RNA PROCESSING|NUCLEUS 0.0180377476870396 0.296189223408368 884 Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping mRNA polyadenylation|snoRNA polyadenylation polynucleotide adenylyltransferase activity nucleoplasm|mRNA cleavage and polyadenylation specificity factor complex YMR296C LCB1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0180266758928854 0.296485837369532 885 Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine sphingolipid biosynthetic process serine C-palmitoyltransferase activity SPOTS complex|serine C-palmitoyltransferase complex YHR057C CPR2 hom 0.0180074710517543 0.297000807045789 886 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity cellular_component YJR031C GEA1 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0179994082139729 0.29721718670545 887 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|Golgi-associated vesicle|extrinsic to membrane YHR134W WSS1 hom FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0179868625040091 0.297554081131484 888 Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response protein sumoylation|response to UV-B|response to UV-C|response to DNA damage stimulus molecular_function nuclear envelope YKR027W BCH2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.0179510695112173 0.2985166447939 889 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function trans-Golgi network transport vesicle|mitochondrion|clathrin-coated vesicle|exomer complex YGL030W RPL30 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0179385908773179 0.299068956159499 890 Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog cytoplasmic translation|negative regulation of mRNA splicing, via spliceosome|negative regulation of translation|rRNA processing structural constituent of ribosome|RNA binding|pre-mRNA 5'-splice site binding cytosolic large ribosomal subunit|cytoplasm YJL004C SYS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0179233587935859 0.299551667208586 891 Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation vesicle organization|Golgi to plasma membrane protein transport|Golgi to endosome transport molecular_function trans-Golgi network|integral to membrane|integral to Golgi membrane YKR039W GAP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PLASMA MEMBRANE 0.0178555949791606 0.301094350387021 892 General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth amino acid transport|gamma-aminobutyric acid transport|ammonia assimilation cycle|polyamine transport amino acid transmembrane transporter activity|polyamine transmembrane transporter activity|L-proline transmembrane transporter activity endosome|fungal-type vacuole lumen|integral to membrane|plasma membrane|ER to Golgi transport vesicle|integral to plasma membrane|multivesicular body YBR226C_d YBR226C hom 0.0178481767429923 0.301295253067853 893 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Unknown Unknown Unknown YAL053W FLC2 hom FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0177950387927238 0.302736957835075 894 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication protein folding|fungal-type cell wall biogenesis|FAD transport|transmembrane transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|cytoplasm YDL175C AIR2 hom FT RNA PROCESSING|NUCLEUS 0.0177738043177188 0.303314358273749 895 RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication nuclear polyadenylation-dependent CUT catabolic process|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|tRNA modification polynucleotide adenylyltransferase activity|RNA binding|protein binding, bridging nucleolus|TRAMP complex YNL265C IST1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0177691840113426 0.303440088884724 896 Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p late endosome to vacuole transport via multivesicular body sorting pathway molecular_function endosome|cytoplasm YLR056W ERG3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0177377640258505 0.304296026955199 897 C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase ergosterol biosynthetic process C-5 sterol desaturase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum lumen YER144C UBP5 hom FT PROTEOLYSIS|SITE OF POLARIZED GROWTH 0.0177240080484705 0.304671268923746 898 Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck protein deubiquitination ubiquitin-specific protease activity incipient cellular bud site|cellular bud neck YDR093W DNF2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0177215778156117 0.304737593847051 899 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity integral to membrane|plasma membrane YGR153W_p YGR153W hom 0.01771923008082 0.304873793026 900 Putative protein of unknown function biological_process molecular_function cellular_component YMR075C-A_d YMR075C-A hom 0.0176621552157196 0.306578762429555 901 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Unknown Unknown Unknown YBR299W MAL32 hom FT CARBOHYDRATE METABOLISM 0.0176293126264495 0.307262743328618 902 Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YJL082W IML2 hom FT NUCLEUS|MITOCHONDRION 0.0175864332063876 0.308513125144374 903 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication biological_process molecular_function mitochondrial outer membrane|mitochondrion|nucleus|cytoplasm YMR237W BCH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.017586000971233 0.308452876188465 904 Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function ribosome|clathrin-coated vesicle|exomer complex YER019W ISC1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0175598514332435 0.309245040759559 905 Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance response to salt stress|ceramide biosynthetic process|sphingolipid catabolic process inositol phosphosphingolipid phospholipase activity mitochondrion|endoplasmic reticulum|integral to mitochondrial outer membrane YPL211W NIP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.0175528464765001 0.309365963531015 906 Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p ribosomal large subunit assembly|ribosomal large subunit biogenesis|rRNA processing molecular_function cytosolic large ribosomal subunit|nucleolus|preribosome, large subunit precursor YBL084C CDC27 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.017536197799619 0.310041657111796 907 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YNL296W_d YNL296W hom 0.0175195724389224 0.310284136247965 908 Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Unknown Unknown Unknown YOR183W_p FYV12 hom 0.0175120330222958 0.310492430569539 909 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cellular_component YDR285W ZIP1 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0174928532705505 0.311022732607852 910 Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate synapsis|reciprocal meiotic recombination chromatin binding|SUMO polymer binding transverse filament YIL078W THS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0174728395340892 0.311576729942872 911 Threonyl-tRNA synthetase, essential cytoplasmic protein cytoplasmic translation|threonyl-tRNA aminoacylation threonine-tRNA ligase activity mitochondrion|cytoplasm YJR083C ACF4 hom 0.0174703383478932 0.311646010604644 912 Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate biological_process molecular_function cellular_component YLR431C ATG23 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0174633484799308 0.31183967763635 913 Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p protein localization to pre-autophagosomal structure|positive regulation of macroautophagy|CVT pathway molecular_function pre-autophagosomal structure|extrinsic to membrane YHR081W LRP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS 0.0174570615345886 0.312664132528846 914 Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination U5 snRNA 3'-end processing|nuclear mRNA surveillance|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|nuclear retention of pre-mRNA at the site of transcription|U4 snRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) double-stranded RNA binding|double-stranded DNA binding nuclear exosome (RNase complex) YGR202C PCT1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.0174530450056753 0.312341835652017 915 Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity phosphatidylcholine biosynthetic process|CDP-choline pathway choline-phosphate cytidylyltransferase activity nuclear envelope|Golgi apparatus|nucleus YER114C BOI2 hom FT SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0174139734759775 0.313209960156694 916 Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication cytokinesis, completion of separation|establishment of cell polarity|budding cell apical bud growth|Rho protein signal transduction phospholipid binding site of polarized growth|cellular bud|cellular bud neck YDL108W KIN28 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0173940199077827 0.313837006720173 917 Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase I promoter|protein phosphorylation|transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activating kinase activity|protein kinase activity TFIIK complex|holo TFIIH complex YDR431W_d YDR431W hom 0.0173795664797014 0.31445596104758 918 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR153C SPO16 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0173141622081569 0.318239253455119 919 Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation synaptonemal complex assembly|ascospore formation|regulation of reciprocal meiotic recombination molecular_function condensed nuclear chromosome YDL145C COP1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0172769449469705 0.317033577536232 920 Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YDR448W ADA2 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.017249265041527 0.317809651679043 921 Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes chromatin silencing at rDNA|chromatin modification|regulation of transcription from RNA polymerase II promoter|positive regulation of histone acetylation|chromatin silencing at telomere chromatin binding|transcription coactivator activity|histone acetyltransferase activity|phosphatidylserine binding SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YDR203W_d YDR203W hom FF 0.0172436605312574 0.318111291342276 922 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR180C DCI1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0172425940671411 0.317996874779106 923 Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed fatty acid beta-oxidation dodecenoyl-CoA delta-isomerase activity peroxisomal matrix YDR182W-A_p YDR182W-A hom 0.0172279027217887 0.318409446905133 924 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YBR157C ICS2 hom 0.0172216916742815 0.318583975016202 925 Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization biological_process molecular_function cellular_component YGR164W_d YGR164W hom 0.0172154459287017 0.318759541261845 926 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL113W SKM1 hom FT CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|PLASMA MEMBRANE 0.0171930287179863 0.319462368535899 927 Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth negative regulation of gene expression|cytokinetic cell separation|protein phosphorylation|sterol import protein serine/threonine kinase activity|protein kinase activity plasma membrane|nucleus YLR125W_p YLR125W hom 0.0171487483796366 0.32063833933566 928 Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene biological_process molecular_function cellular_component YGR250C YGR250C hom 0.0171227839059886 0.322166621586832 929 Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress biological_process RNA binding cytoplasmic stress granule|cytoplasm YKL161C KDX1 hom FT PROTEIN PHOSPHORYLATION 0.0170961820364659 0.322412924367511 930 Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p biological_process protein kinase activity cellular_component YDR004W RAD57 hom FF|FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0170580842627577 0.323203826042679 931 Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p DNA recombinase assembly|telomere maintenance via recombination|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YBL060W YEL1 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|SIGNALING|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0170551900629564 0.323285941766317 932 Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip positive regulation of phosphatidylinositol biosynthetic process|protein localization|actin cortical patch localization ARF guanyl-nucleotide exchange factor activity cellular bud neck|cellular bud tip|cytoplasm YOR381W FRE3 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.0170519590016972 0.323377631153151 933 Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular iron ion homeostasis|siderophore transport ferric-chelate reductase activity integral to membrane|plasma membrane YDR289C RTT103 hom FT RNA PROCESSING|NUCLEUS|CHROMOSOME 0.0170400021218657 0.323717084644713 934 Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated|mRNA 3'-end processing RNA polymerase II core binding nuclear chromatin|site of double-strand break YAL012W CYS3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0170283646942776 0.324842526488836 935 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress sulfur amino acid metabolic process|cysteine biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|cysteine metabolic process cystathionine gamma-lyase activity cytoplasm YLR131C ACE2 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|NUCLEUS 0.0170091462497605 0.325244254499674 936 Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YOR036W PEP12 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.017006694164581 0.324736063016899 937 Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Golgi to vacuole transport|vacuole inheritance SNAP receptor activity endosome|Golgi apparatus YBL004W UTP20 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0169639875483545 0.325880548637966 938 Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|small-subunit processome|preribosome, small subunit precursor|nucleolus|90S preribosome|cytoplasm YNR014W_p YNR014W hom 0.0169421867449201 0.326502754969918 939 Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication biological_process molecular_function cytoplasm YOR157C PUP1 het FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0169329280874077 0.32748956632962 940 Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YAL004W_d YAL004W hom 0.016916952319073 0.327657049986107 941 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Unknown Unknown Unknown YOR319W HSH49 het FF|FT RNA PROCESSING|NUCLEUS 0.0169167177249075 0.327230630670355 942 U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YHR005C-A TIM10 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0168991107746024 0.327734432506999 943 Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity|unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex|mitochondrial intermembrane space protein transporter complex YLR077W FMP25 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0168662006988529 0.328677463374215 944 Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|integral to mitochondrial inner membrane YCR075C ERS1 hom 0.0168509460631086 0.329115177426246 945 Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains L-cystine transport|transmembrane transport L-cystine transmembrane transporter activity endosome|integral to membrane|fungal-type vacuole YJL194W CDC6 het FT CELL CYCLE|CELL DIVISION|GENE SILENCING|NUCLEUS|PLASMA MEMBRANE 0.0168507493183971 0.329120825252516 946 Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of DNA-dependent DNA replication initiation|regulation of chromatin silencing at telomere|pre-replicative complex assembly|G1/S transition of mitotic cell cycle DNA replication origin binding|protein binding|chromatin binding|ATPase activity|GTP binding|GTPase activity|ATP binding DNA replication preinitiation complex|pre-replicative complex YOR185C GSP2 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS 0.0168297076399458 0.329941636197304 947 GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress nucleus organization GTPase activity integral to membrane|nucleus YJL030W MAD2 hom FT CELL CYCLE|CELL DIVISION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|NUCLEAR PORE|KINETOCHORE 0.0168220458090736 0.329945471480993 948 Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I chromosome decondensation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex|condensed nuclear chromosome kinetochore|nuclear pore YIL002W-A_p YIL002W-A hom 0.0167970765044392 0.330663921468475 949 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YDR114C_p YDR114C hom 0.01679531151858 0.330714744245322 950 Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium biological_process molecular_function cellular_component YER026C CHO1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0167402508365036 0.33230275365201 951 Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline phosphatidylserine biosynthetic process CDP-diacylglycerol-serine O-phosphatidyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YBR190W_d YBR190W het 0.016737715286308 0.332376000047257 952 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Unknown Unknown Unknown YBR217W ATG12 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0167333780008508 0.333078666738964 953 Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation C-terminal protein lipidation|piecemeal microautophagy of nucleus|mitochondrion degradation|CVT pathway|autophagic vacuole assembly Atg8 ligase activity|protein tag Atg12-Atg5-Atg16 complex|pre-autophagosomal structure YFR049W YMR31 hom FT TRANSLATION|MITOCHONDRION|RIBOSOME 0.0167256068585024 0.33272592928596 954 Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 cytoplasmic translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YBR084C-A RPL19A hom FT TRANSLATION|RIBOSOME 0.0167102027741338 0.334617196529795 955 Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YPL207W TYW1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0166040583464928 0.336251805142066 956 Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron wybutosine biosynthetic process molecular_function endoplasmic reticulum YDL202W MRPL11 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0165846301470508 0.336817597244321 957 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YNL122C_p YNL122C hom FT TRANSLATION|MITOCHONDRION 0.0165642556409942 0.337627798334824 958 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene biological_process molecular_function mitochondrion YDR287W INM2 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM 0.0165622734528986 0.337974166067383 959 Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy inositol phosphate dephosphorylation inositol monophosphate 1-phosphatase activity cellular_component YDR319C_p YFT2 hom 0.0165450103431225 0.337973308719819 960 Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function integral to membrane YPR144C NOC4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0165320434783543 0.33835210508968 961 Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits ribosomal small subunit biogenesis|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|90S preribosome|Noc4p-Nop14p complex YDL009C_p YDL009C hom 0.0165234396223503 0.338603596766001 962 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene biological_process molecular_function cellular_component YOR349W CIN1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0165110447054087 0.338966112374571 963 Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl protein folding|tubulin complex assembly|tubulin complex biogenesis beta-tubulin binding cellular_component YEL012W UBC8 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS 0.0165089246596173 0.339028142526885 964 Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YPL201C YIG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0164894326835871 0.339598796933837 965 Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol glycerol biosynthetic process molecular_function cytosol|nucleus YMR200W ROT1 het FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0164791445975257 0.339900242897351 966 Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation budding cell apical bud growth|cell cycle cytokinesis|establishment or maintenance of actin cytoskeleton polarity|protein O-linked mannosylation|endoplasmic reticulum unfolded protein response|protein folding|fungal-type cell wall biogenesis|'de novo' protein folding|protein N-linked glycosylation unfolded protein binding endoplasmic reticulum|endoplasmic reticulum membrane YGR184C UBR1 hom FT PROTEOLYSIS 0.0164755336252523 0.340799338147514 967 E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) regulation of dipeptide transport|ubiquitin-dependent protein catabolic process via the N-end rule pathway|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process|protein polyubiquitination ubiquitin-protein ligase activity proteasome regulatory particle, base subcomplex|cytoplasm YBR196C-B_p YBR196C-B hom 0.0164163959742967 0.341742518771671 968 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YMR261C TPS3 hom FT CARBOHYDRATE METABOLISM 0.016414831087785 0.341788544720152 969 Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication trehalose biosynthetic process|response to stress enzyme regulator activity|alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity|trehalose-phosphatase activity alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) YLR009W RLP24 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0164140847889465 0.342242718643864 970 Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp ribosomal large subunit biogenesis molecular_function nucleolus|preribosome, large subunit precursor YGR107W_d YGR107W hom 0.0164121444566974 0.341867572311489 971 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR310C_p YMR310C hom FT NUCLEUS 0.0163985967422886 0.342266258047257 972 Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity nucleus YLR448W RPL6B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0163772400060772 0.343111324344659 973 Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YKL143W LTV1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0163583790999738 0.343451543425996 974 Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature ribosomal small subunit biogenesis|cellular response to oxidative stress|response to osmotic stress|ribosomal small subunit export from nucleus molecular_function preribosome, small subunit precursor|EGO complex|nucleus|late endosome membrane|cytoplasm YKR081C RPF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0163430233023358 0.344120721493526 975 Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles ribosomal large subunit assembly|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 7S RNA binding|5S rRNA binding|rRNA binding nucleolus|preribosome, large subunit precursor YNR029C_p YNR029C hom 0.0163204520858659 0.344571718013087 976 Putative protein of unknown function, deletion confers reduced fitness in saline biological_process molecular_function cytoplasm YPL117C IDI1 het FT LIPID METABOLISM|NUCLEUS 0.0162412863505422 0.346917376324349 977 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability farnesyl diphosphate biosynthetic process isopentenyl-diphosphate delta-isomerase activity nucleus|cytoplasm YPR132W RPS23B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0162383037620765 0.347005947702427 978 Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit YEL047C FRD1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0162252995854976 0.347392289682142 979 Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity cytosol|mitochondrion|ribosome|plasma membrane YIL072W HOP1 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0162074627779231 0.347922649326506 980 Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments synaptonemal complex assembly|meiotic recombination checkpoint|synapsis four-way junction DNA binding condensed nuclear chromosome|lateral element YPL057C SUR1 hom FT LIPID METABOLISM 0.0162051778247393 0.347990627289868 981 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication glycosphingolipid biosynthetic process|mannosyl-inositol phosphorylceramide metabolic process|sphingolipid biosynthetic process mannosyltransferase activity|transferase activity, transferring glycosyl groups integral to membrane|intracellular YGR271C-A EFG1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0162024356507983 0.348072218834329 982 Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus G1 phase of mitotic cell cycle|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YDR138W HPR1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.016201491067224 0.348100327099106 983 Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|response to DNA damage stimulus|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|Cdc73/Paf1 complex|nucleoplasmic THO complex YOR345C_d YOR345C hom 0.0161161623786785 0.350645427964014 984 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YEL022W GEA2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0161153787529757 0.350668855703825 985 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|secretory granule organization|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|extrinsic to membrane YGR129W SYF2 hom FT RNA PROCESSING|NUCLEUS 0.0161046356024109 0.35099013933884 986 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest mRNA splicing, via spliceosome first spliceosomal transesterification activity|second spliceosomal transesterification activity U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YMR205C PFK2 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0160907928843978 0.351619920899534 987 Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes proton transport|glycolysis mRNA binding|6-phosphofructokinase activity mitochondrion|6-phosphofructokinase complex|cytoplasm YPL093W NOG1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0160892033246008 0.35145198180669 988 Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|rRNA processing GTP binding nucleolus|preribosome, large subunit precursor YDR442W_d YDR442W hom 0.0159810879757801 0.35491366294062 989 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHL022C SPO11 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0159763843517937 0.354840001525108 990 Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic DNA double-strand break formation endodeoxyribonuclease activity, producing 3'-phosphomonoesters nuclear chromosome YMR316C-A_p YMR316C-A hom 0.0159275179751391 0.356313862738305 991 Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W biological_process molecular_function cellular_component YJL008C CCT8 het 0.0158866058504294 0.357550777332954 992 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YFL039C ACT1 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MEMBRANE ORGANIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPON 0.0158849309742616 0.357601472117462 993 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions ascospore wall assembly|vacuole inheritance|protein secretion|DNA repair|budding cell isotropic bud growth|mitochondrion inheritance|fungal-type cell wall organization|vesicle transport along actin filament|establishment of cell polarity|chronological cell aging|cytokinesis|actin filament reorganization involved in cell cycle|histone acetylation|endocytosis|exocytosis|establishment of mitotic spindle orientation|regulation of transcription from RNA polymerase II promoter|actomyosin contractile ring contraction|cellular response to oxidative stress structural constituent of cytoskeleton cellular bud neck contractile ring|actin filament|histone acetyltransferase complex|Swr1 complex|actin filament bundle|NuA4 histone acetyltransferase complex|Ino80 complex|actin cortical patch YPL172C COX10 hom FT COFACTOR METABOLISM|MITOCHONDRION 0.0158632516953554 0.358544883689653 994 Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders heme a biosynthetic process protoheme IX farnesyltransferase activity mitochondrion|integral to membrane YBR033W_p EDS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0158487557864326 0.358697518332314 995 Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication biological_process sequence-specific DNA binding cellular_component YGR200C ELP2 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0158398628280594 0.358967282710537 996 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin regulation of transcription from RNA polymerase II promoter|protein urmylation|tRNA wobble uridine modification molecular_function Elongator holoenzyme complex|nucleus|cytoplasm YMR224C MRE11 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0158307207758702 0.359244736093129 997 Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress DNA repair|meiotic DNA double-strand break formation|double-strand break repair via nonhomologous end joining|base-excision repair|ascospore formation|meiotic DNA double-strand break processing|double-strand break repair via break-induced replication|regulation of transcription during meiosis 3'-5' exonuclease activity|protein complex scaffold|double-stranded telomeric DNA binding|adenylate kinase activity|telomeric DNA binding|single-stranded telomeric DNA binding|endodeoxyribonuclease activity|G-quadruplex DNA binding|endonuclease activity mitochondrion|nucleus|Mre11 complex YBR240C THI2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0157615273530855 0.361349062528136 998 Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type thiamine biosynthetic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription nucleus YMR207C HFA1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0157472211633825 0.361785107557775 999 Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress fatty acid biosynthetic process acetyl-CoA carboxylase activity mitochondrion YBL078C ATG8 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM 0.0157210306092841 0.362584235369754 1000 Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis piecemeal microautophagy of nucleus|mitochondrion degradation|cellular membrane fusion|ER to Golgi vesicle-mediated transport|CVT pathway|autophagic vacuole assembly molecular_function cytosol|fungal-type vacuole membrane|pre-autophagosomal structure|extrinsic to membrane|autophagic vacuole YPL137C GIP3 hom FT CHROMOSOME SEGREGATION|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0157161490193072 0.362733304813535 1001 Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication chromosome segregation|regulation of phosphoprotein phosphatase activity protein phosphatase type 1 regulator activity|protein phosphatase 1 binding mitochondrion|ribosome|endoplasmic reticulum|cytoplasm YLR258W GSY2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0156786984272463 0.363878211601971 1002 Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress glycogen biosynthetic process glycogen (starch) synthase activity cytoplasm YOR374W ALD4 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0156625976571499 0.364371123837965 1003 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed NADPH regeneration|ethanol metabolic process|pyruvate metabolic process|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity|aldehyde dehydrogenase (NAD) activity mitochondrial nucleoid|mitochondrion YNL100W AIM37 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0156613424285166 0.364409569182069 1004 Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YDL238C GUD1 hom 0.0156599141344741 0.364453318283559 1005 Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures guanine metabolic process guanine deaminase activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides cytoplasm YGR175C ERG1 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0156120863665172 0.365920192140343 1006 Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine ergosterol biosynthetic process drug binding|squalene monooxygenase activity endoplasmic reticulum|lipid particle YIL016W SNL1 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.015600133610936 0.366287356569847 1007 Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein protein folding|nuclear pore organization ribosome binding nuclear envelope|mitochondrion|cytosolic ribosome|endoplasmic reticulum membrane|membrane YBR041W FAT1 hom FT KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0155862398537129 0.366714433208723 1008 Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids very long-chain fatty acid metabolic process|long-chain fatty acid transport very long-chain fatty acid-CoA ligase activity|long-chain fatty acid transporter activity intrinsic to internal side of plasma membrane|integral to membrane|endoplasmic reticulum|lipid particle|peroxisome YHR098C SFB3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.0155475511946329 0.367905308327366 1009 Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat|mating projection tip YHR187W IKI1 hom FT RNA PROCESSING|NUCLEUS 0.0155100758035141 0.369061130098618 1010 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin tRNA modification tRNA binding|ATPase activity Elongator holoenzyme complex|nucleus|cytoplasm YKR092C SRP40 hom FT NUCLEUS 0.0154715400633977 0.370252006568516 1011 Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 nucleocytoplasmic transport molecular_function nucleolus YLR030W_p YLR030W hom 0.0154181317778026 0.371906431424265 1012 Putative protein of unknown function biological_process molecular_function cellular_component YGR007W ECT1 hom FT LIPID METABOLISM|NUCLEUS 0.0154089270973753 0.372192026360874 1013 Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases phosphatidylethanolamine biosynthetic process ethanolamine-phosphate cytidylyltransferase activity nucleus|cytoplasm YDR346C SVF1 hom FT RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0154071422601035 0.372247420487111 1014 Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance cellular response to oxidative stress molecular_function nucleus|cytoplasm YAR014C BUD14 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0153824910771027 0.373155586693587 1015 Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress regulation of cell shape during vegetative growth phase|regulation of protein localization|regulation of transcription, DNA-dependent|cytoskeleton organization|negative regulation of actin filament polymerization protein phosphatase type 1 regulator activity incipient cellular bud site|cellular bud neck|cellular bud tip YFR001W LOC1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS 0.0153771777133701 0.373178161318669 1016 Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|intracellular mRNA localization mRNA binding nucleolus|preribosome, large subunit precursor YLL045C RPL8B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0153378123541752 0.37440309145118 1017 Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR264C-A_p YLR264C-A hom 0.0153370266373746 0.3744275658366 1018 Putative protein of unknown function biological_process molecular_function cellular_component YJL077C ICS3 hom 0.0152704388894226 0.376576472699651 1019 Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 biological_process molecular_function cellular_component YPR083W MDM36 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0151550101952953 0.380194887182759 1020 Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission mitochondrial fission|mitochondrion organization molecular_function mitochondrion|cytoplasm YMR154C RIM13 hom FF|FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS 0.0151432803738017 0.381275131347803 1021 Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB protein processing cysteine-type endopeptidase activity cellular_component YER088C DOT6 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0151394211275601 0.380614171375817 1022 Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress chromatin silencing at rDNA|filamentous growth|regulation of transcription from RNA polymerase II promoter|unidimensional cell growth|chromatin silencing at telomere sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|Rpd3L complex|cytoplasm YMR230W-A_p YMR230W-A hom 0.0151355573969593 0.380735758748903 1023 Putative protein of unknown function biological_process molecular_function cellular_component YDR482C CWC21 hom FT RNA PROCESSING|NUCLEUS 0.015115190368413 0.381448082091604 1024 Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YER178W PDA1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0150849510818811 0.382330484760517 1025 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose acetyl-CoA biosynthetic process from pyruvate pyruvate dehydrogenase (acetyl-transferring) activity mitochondrial nucleoid|mitochondrion|mitochondrial pyruvate dehydrogenase complex YOL039W RPP2A hom FT TRANSLATION|RIBOSOME 0.0150696011410093 0.382885956634776 1026 Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL026W_d YDL026W hom 0.0150684584843074 0.383063992847991 1027 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR218C PYC2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0150668988488777 0.38290034292438 1028 Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication NADPH regeneration|gluconeogenesis pyruvate carboxylase activity cytosol YOL038W PRE6 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0150462490414651 0.383552836689226 1029 Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YDR294C DPL1 hom FT KETONE METABOLISM|LIPID METABOLISM|SIGNALING|RESPONSE TO NUTRIENT LEVELS|ENDOPLASMIC RETICULUM 0.0149948575826475 0.385250523809003 1030 Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate calcium-mediated signaling|sphingolipid metabolic process|cellular response to starvation sphinganine-1-phosphate aldolase activity endoplasmic reticulum|cortical endoplasmic reticulum|perinuclear endoplasmic reticulum YNL027W CRZ1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|NUCLEUS 0.0149725468259427 0.385887227034894 1031 Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress regulation of potassium ion concentration by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to calcium ion|positive regulation of sodium ion transport by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|cell wall chitin biosynthetic process|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter by pheromones|ion homeostasis|Unknown|positive regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YBL034C STU1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0149504025829295 0.3865902985996 1032 Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles microtubule nucleation structural constituent of cytoskeleton spindle pole body YPR108W-A_p YPR108W-A hom 0.0149362789154947 0.387251571394944 1033 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YDL089W NUR1 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.0149063119408894 0.388275635926652 1034 Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate maintenance of rDNA molecular_function nuclear periphery YOR147W MDM32 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0149001176524269 0.388189725887852 1035 Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 mitochondrion inheritance|cellular ion homeostasis|mitochondrion organization molecular_function mitochondrion|mitochondrial inner membrane YDR007W TRP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0148948352943615 0.388357976930495 1036 Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) tryptophan biosynthetic process phosphoribosylanthranilate isomerase activity cytoplasm YCL032W STE50 hom FT CELL CYCLE|SIGNALING 0.0148883069069086 0.388919852282994 1037 Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction pheromone-dependent signal transduction involved in conjugation with cellular fusion|MAPK cascade involved in osmosensory signaling pathway|osmosensory signaling pathway via Sho1 osmosensor|signal transduction involved in filamentous growth|cellular response to heat SAM domain binding|protein kinase regulator activity cytoplasm YNL090W RHO2 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0148749220445374 0.388992642909463 1038 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly microtubule-based process|fungal-type cell wall organization|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction GTPase activity membrane YPR191W QCR2 hom FT OXIDATION-REDUCTION PROCESS|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0148727095497315 0.389063197459236 1039 Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial crista|mitochondrial respiratory chain complex III YOR188W MSB1 hom FT MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0148673047584204 0.389235584323239 1040 Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress establishment of cell polarity molecular_function mitochondrion|cellular bud neck|cellular bud tip YLR119W SRN2 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0148272614239131 0.390514220806918 1041 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole protein binding endosome|ESCRT I complex YJR115W_p YJR115W hom 0.0148253233823337 0.390788205594948 1042 Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication biological_process molecular_function cellular_component YDR323C PEP7 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.0147917038407654 0.391651757082997 1043 Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Golgi to vacuole transport|vacuole inheritance|vesicle fusion|vesicle docking involved in exocytosis|Golgi to endosome transport phosphatidylinositol-3-phosphate binding external side of endosome membrane|cytoplasm YLR087C CSF1 hom FF|FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0147617188329533 0.392824372612142 1044 Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies fermentation molecular_function mitochondrion YKR020W VPS51 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL DIVISION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0147591734890626 0.392835377337705 1045 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p vesicle organization|retrograde transport, endosome to Golgi|budding cell apical bud growth|regulation of cell size|protein targeting to vacuole protein binding Golgi apparatus|GARP complex YLR436C ECM30 hom FT CELL WALL ORG/BIOGENESIS 0.0147562348544782 0.392788459220704 1046 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization molecular_function cytoplasm YBR029C CDS1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0147477576389195 0.393060430772583 1047 Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids phosphatidylserine metabolic process|phosphatidylglycerol biosynthetic process phosphatidate cytidylyltransferase activity mitochondrion|integral to membrane|endoplasmic reticulum YER002W NOP16 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.014691952890516 0.394924128486212 1048 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YPL060W MFM1 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0146788727080217 0.395274668164697 1049 Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity integral to membrane|mitochondrial inner membrane YMR100W MUB1 hom FT CELL DIVISION 0.0146440323018501 0.396749744110996 1050 MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene regulation of cell budding|protein monoubiquitination molecular_function cellular_component YLR312W-A MRPL15 hom FT RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0146273327796048 0.397147544907477 1051 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YJL127C SPT10 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS 0.0146222218684999 0.397101288018728 1052 Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box DNA repair|negative regulation of transcription from RNA polymerase II promoter|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette histone acetyltransferase activity|sequence-specific DNA binding nucleus YIL129C TAO3 het FT CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0145958870744666 0.397952143245355 1053 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p cell morphogenesis|cell budding|budding cell apical bud growth molecular_function incipient cellular bud site|mitochondrion|cellular bud|mating projection tip YDR535C_d YDR535C hom 0.014582872309013 0.398373044610944 1054 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Unknown Unknown Unknown YLR339C_d YLR339C het 0.0145673357608208 0.399298035782803 1055 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Unknown Unknown Unknown YHL027W RIM101 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CELL DIVISION|ION HOMEOSTASIS|NUCLEUS 0.0145640334723416 0.399053087180345 1056 Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC meiosis|negative regulation of transcription from RNA polymerase II promoter|fungal-type cell wall biogenesis|cellular response to anoxia|response to pH|ascospore formation|barrier septum assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding nucleus YDL180W_p YDL180W hom 0.0145437269474945 0.39964063167608 1057 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function integral to membrane|fungal-type vacuole YKR070W_p YKR070W hom FT MITOCHONDRION 0.0145324612665996 0.400216744887136 1058 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOL005C RPB11 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0145259167614588 0.40028841556341 1059 RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase II, core complex YMR315W YMR315W hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0145154920225847 0.400556425512871 1060 Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress NADPH regeneration oxidoreductase activity nucleus|cytoplasm YGR174C CBP4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0144729068451812 0.402010233605305 1061 Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|integral to mitochondrial membrane YLR347C KAP95 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0144675649200154 0.402394630249929 1062 Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p protein targeting to membrane|regulation of protein desumoylation|protein import into nucleus|phosphatidylcholine biosynthetic process|nuclear pore complex assembly protein transporter activity|Ran guanyl-nucleotide exchange factor activity integral to membrane|nuclear exosome (RNase complex)|nuclear pore|cytoplasm YGR128C UTP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.0144165716164433 0.403774817314126 1063 Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|tRNA export from nucleus snoRNA binding|tRNA binding small-subunit processome|t-UTP complex|nucleolus|rDNA heterochromatin|90S preribosome YER074W RPS24A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0144021917581195 0.404243953260166 1064 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|mitochondrion YPR051W MAK3 hom FT NUCLEUS 0.0143575941655206 0.405701000231033 1065 Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatC complex YKL168C KKQ8 hom FT PROTEIN PHOSPHORYLATION 0.0143420257386086 0.406700685699786 1066 Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication biological_process protein kinase activity cytoplasm YDR467C_d YDR467C hom 0.0143340637014731 0.406471025612076 1067 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML035C AMD1 hom FT NUCLEOTIDE METABOLISM 0.0143134394246102 0.407846937976106 1068 AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools guanine salvage|purine nucleotide metabolic process AMP deaminase activity cytoplasm YNL298W CLA4 hom FF|FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0142644453283981 0.409593989856899 1069 Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p negative regulation of gene expression|vacuole inheritance|protein phosphorylation|budding cell apical bud growth|Rho protein signal transduction|regulation of exit from mitosis|establishment of cell polarity|sterol import|response to pheromone|cytokinesis protein serine/threonine kinase activity|protein kinase activity nucleus|cellular bud|fungal-type vacuole YLR069C MEF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0142359220360061 0.409692063733499 1070 Mitochondrial elongation factor involved in translational elongation mitochondrial translational elongation|mitochondrial translation translation elongation factor activity mitochondrion YKR074W AIM29 hom 0.0141981038923613 0.41128631969508 1071 Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cytoplasm YBR162C TOS1 hom 0.014182729682115 0.411932745070825 1072 Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C biological_process molecular_function fungal-type cell wall|fungal-type vacuole YKL098W MTC2 hom 0.0141534426071395 0.412759415692141 1073 Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YOR068C VAM10 hom FT MEMBRANE ORGANIZATION 0.014127069765701 0.413282314526763 1074 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole fusion, non-autophagic molecular_function fungal-type vacuole membrane YEL009C GCN4 hom FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|AMINO ACID METABOLISM|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0141231838622434 0.413410825787377 1075 Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels nitrogen catabolite activation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription initiation from RNA polymerase II promoter|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels chromatin binding|TFIID-class binding transcription factor activity|sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding transcription factor activity nucleus YGR134W CAF130 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0141057771318376 0.41398677641087 1076 Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex YGL197W MDS3 hom FT CELL CYCLE|SIGNALING|MITOCHONDRION 0.014096916652541 0.414280132465873 1077 Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain TOR signaling cascade|negative regulation of sporulation resulting in formation of a cellular spore molecular_function mitochondrion|cytoplasm YDL160C-A MHF2 hom FT RESPONSE TO DNA DAMAGE 0.0140747815514868 0.415013526496642 1078 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 response to DNA damage stimulus molecular_function cellular_component YAL064C-A_p TDA8 hom 0.0140408792112006 0.416138285836938 1079 Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process molecular_function cellular_component YDR373W FRQ1 het FT SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0139765115678524 0.418278717920837 1080 N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily regulation of signal transduction enzyme activator activity|calcium ion binding Golgi membrane YER183C FAU1 hom FT COFACTOR METABOLISM|MITOCHONDRION 0.0139543697619973 0.41908586343094 1081 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis folic acid-containing compound biosynthetic process 5-formyltetrahydrofolate cyclo-ligase activity mitochondrion YNL108C_p YNL108C hom FT NUCLEUS 0.0139195883156766 0.420176987492063 1082 Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YDR281C PHM6 hom 0.0138994674097657 0.420849185207007 1083 Protein of unknown function, expression is regulated by phosphate levels biological_process molecular_function fungal-type vacuole YGR089W NNF2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0138431013359983 0.422735615224277 1084 Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation biological_process molecular_function integral to membrane YBR056W YBR056W hom FT CARBOHYDRATE METABOLISM 0.0138186265789818 0.42390205476945 1085 Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function cytoplasm YMR306W FKS3 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0137686678239206 0.425234286600773 1086 Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies ascospore wall assembly 1,3-beta-D-glucan synthase activity mitochondrion|integral to membrane YOR243C PUS7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0137357102461908 0.42641236674086 1087 Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria snRNA pseudouridine synthesis|tRNA pseudouridine synthesis|enzyme-directed rRNA pseudouridine synthesis pseudouridine synthase activity nucleus YOR198C BFR1 hom FT CELL CYCLE|ENDOMEMBRANE SYSTEM 0.0137103353830103 0.427198469994971 1088 Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity meiosis|mRNA metabolic process|regulation of mitosis RNA binding ribonucleoprotein complex|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YGR020C VMA7 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0137036008781913 0.427425575516679 1089 Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane vacuolar acidification proton-transporting ATPase activity, rotational mechanism fungal-type vacuole membrane|vacuolar proton-transporting V-type ATPase, V1 domain|integral to membrane YDL209C CWC2 het FT CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0136830614420564 0.42887720735769 1090 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 mRNA cis splicing, via spliceosome|mRNA splicing, via spliceosome|spliceosomal snRNP assembly pre-mRNA binding|first spliceosomal transesterification activity|U6 snRNA binding|second spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome|U2-type catalytic step 2 spliceosome YDR478W SNM1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0136741520133521 0.428419495440557 1091 Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP mRNA cleavage|rRNA processing ribonuclease MRP activity|RNA binding ribonuclease MRP complex YGL175C SAE2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0136445143227856 0.42976532596351 1092 Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents DNA double-strand break processing|DNA catabolic process, endonucleolytic|meiotic DNA double-strand break formation|meiotic DNA double-strand break processing|gene conversion at mating-type locus, DNA double-strand break processing double-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleus|cytoplasm YDR261C EXG2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0136415765515099 0.429589301800357 1093 Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor fungal-type cell wall organization glucan exo-1,3-beta-glucosidase activity fungal-type cell wall YBR171W SEC66 hom FF|FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0136259424506513 0.430255853168734 1094 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation|filamentous growth protein transporter activity integral to membrane|Sec62/Sec63 complex|endoplasmic reticulum membrane YPL065W VPS28 hom FF|FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0135366523583808 0.43307795911314 1095 Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole protein complex binding endosome|ESCRT I complex YDR016C DAD1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0135162436894066 0.433771889233091 1096 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton condensed nuclear chromosome kinetochore|DASH complex|spindle YPR112C MRD1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0134794093320384 0.43502594609896 1097 Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|small-subunit processome assembly|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|90S preribosome YMR179W SPT21 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS 0.0134657976271204 0.435489896905373 1098 Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress chromatin silencing at rDNA|regulation of transcription from RNA polymerase II promoter|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette molecular_function nucleus YPL232W SSO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0134537346360991 0.435901298637575 1099 Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p ascospore formation|ascospore-type prospore assembly|vesicle fusion phosphatidic acid binding|phosphatidylinositol-3,4-bisphosphate binding|phosphatidylinositol-4,5-bisphosphate binding|phosphatidylinositol-3,5-bisphosphate binding|SNAP receptor activity SNARE complex|fungal-type vacuole membrane|plasma membrane|prospore membrane YGR287C IMA1 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0134391103430671 0.436400352435462 1100 Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family disaccharide catabolic process oligo-1,6-glucosidase activity mitochondrion|ribosome YMR300C ADE4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0133838354036155 0.438699566634645 1101 Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway 'de novo' IMP biosynthetic process amidophosphoribosyltransferase activity cytoplasm YLR168C UPS2 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0132953567077411 0.44132342952185 1102 Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI cristae formation|Unknown|phosphatidylethanolamine metabolic process|cardiolipin metabolic process molecular_function extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial intermembrane space YHL028W WSC4 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0132948149608072 0.441682738839896 1103 ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 response to heat|SRP-dependent cotranslational protein targeting to membrane, translocation|protein targeting to ER|response to stress transmembrane signaling receptor activity endoplasmic reticulum membrane YFR034C PHO4 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0132582390167123 0.44287194902908 1104 Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability cellular response to phosphate starvation|positive regulation of phosphate metabolic process|phosphate-containing compound metabolic process|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YNL121C TOM70 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0132447118603379 0.443065390429081 1105 Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication protein import into mitochondrial matrix|protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YPL142C_d YPL142C het 0.0132158526940729 0.444739901912684 1106 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Unknown Unknown Unknown YHR141C RPL42B hom FT TRANSLATION|RIBOSOME 0.0131868298003904 0.445061081862058 1107 Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR226W ADK1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0131593939615261 0.446008817590883 1108 Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress ADP biosynthetic process|DNA replication initiation|nucleotide metabolic process adenylate kinase activity mitochondrion|mitochondrial intermembrane space|cytoplasm YGL113W SLD3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0131556943192087 0.446136704821313 1109 Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress DNA unwinding involved in replication|double-strand break repair via break-induced replication|DNA replication initiation chromatin binding nuclear chromatin|DNA replication preinitiation complex YLR244C MAP1 hom FT PROTEOLYSIS|RIBOSOME 0.013102241905598 0.449139727506201 1110 Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p negative regulation of gene expression|protein initiator methionine removal involved in protein maturation metalloaminopeptidase activity|mRNA binding cytosolic ribosome YGL027C CWH41 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0130536783370584 0.449671351259379 1111 Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress fungal-type cell wall biogenesis|protein N-linked glycosylation mannosyl-oligosaccharide glucosidase activity endoplasmic reticulum membrane YNL089C_d YNL089C hom 0.0130511856788732 0.449757914959072 1112 Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Unknown Unknown Unknown YDL019C OSH2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0130425273958504 0.450058668530494 1113 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability endocytosis|maintenance of cell polarity|sterol transport|exocytosis lipid binding|oxysterol binding|sterol transporter activity|1-phosphatidylinositol binding endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum|cellular bud neck YHL025W SNF6 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|NUCLEUS|RSC COMPLEX 0.0130338735634688 0.450359381301382 1114 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|nucleotide-excision repair|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YMR123W PKR1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0130058346512624 0.453026259508619 1115 V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress vacuolar proton-transporting V-type ATPase complex assembly molecular_function integral to endoplasmic reticulum membrane YPL090C RPS6A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0130034688745129 0.45141681803992 1116 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YPL205C_d YPL205C hom 0.0129841729318481 0.452088634186895 1117 Hypothetical protein; deletion of locus affects telomere length Unknown Unknown Unknown YKR035W-A DID2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.0129640998915636 0.452855491914829 1118 Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors late endosome to vacuole transport via multivesicular body sorting pathway|late endosome to vacuole transport|protein targeting to vacuole molecular_function late endosome|cytoplasm YPL043W NOP4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0129184576737904 0.454380838888039 1119 Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) rRNA processing RNA binding nucleolus YGR029W ERV1 het FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0129127071454931 0.454581733333497 1120 Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) cellular response to oxidative stress|cellular iron ion homeostasis|protein import into mitochondrial intermembrane space thiol oxidase activity|flavin-linked sulfhydryl oxidase activity mitochondrion|mitochondrial intermembrane space YLR167W RPS31 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION|NUCLEUS|RIBOSOME 0.0128979561755087 0.455097286487407 1121 Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S)|ribosomal small subunit assembly|ribosome biogenesis structural constituent of ribosome|protein tag cytosolic small ribosomal subunit|cytoplasm YBL090W MRP21 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0128885906889027 0.455424785269368 1122 Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial translational initiation|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YNR062C_p YNR062C hom 0.0128596053773696 0.456439203388832 1123 Putative membrane protein of unknown function biological_process molecular_function integral to membrane YHL032C GUT1 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0128446787018085 0.456962097007181 1124 Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p glycerol metabolic process glycerol kinase activity mitochondrion|cytoplasm YBR049C REB1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0128166557576289 0.457944671553742 1125 RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication termination of RNA polymerase I transcription|regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nucleoplasm YOL052C-A DDR2 hom 0.0127910071420713 0.458845032158925 1126 Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication response to stress molecular_function fungal-type vacuole|cytoplasm YNL244C SUI1 het FT TRANSLATION 0.0127776718879568 0.459581449650224 1127 Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase translational initiation translation initiation factor binding|translation initiation factor activity multi-eIF complex YDR079C-A TFB5 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0127624078278702 0.45985014145157 1128 Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleus YJR045C SSC1 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0127336479456191 0.460862134884074 1129 Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication protein refolding|positive regulation of endodeoxyribonuclease activity|protein import into mitochondrial matrix|protein unfolding enzyme regulator activity|ATPase activity mitochondrial nucleoid|mitochondrion|TRC complex|mitochondrial inner membrane|presequence translocase-associated import motor YGL194C HOS2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|NUCLEUS|CHROMOSOME 0.0127169047899178 0.461451860287272 1130 Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS negative regulation of meiosis|chromatin organization|regulation of transcription, DNA-dependent|positive regulation of stress-activated MAPK cascade|histone deacetylation NAD-independent histone deacetylase activity|NAD-dependent histone deacetylase activity Rpd3L-Expanded complex|Set3 complex YLR031W_p YLR031W hom 0.012715802676422 0.461490693570285 1131 Putative protein of unknown function biological_process molecular_function cellular_component YJR076C CDC11 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0126968920299455 0.462424435217739 1132 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells maintenance of cell polarity|cell cycle cytokinesis|positive regulation of protein kinase activity structural molecule activity|GTP binding|1-phosphatidylinositol binding spindle microtubule|septin complex|prospore membrane|ascospore wall|mating projection base|meiotic spindle|cellular bud neck septin ring|septin filament array|cytoplasmic microtubule|mating projection tip YCR044C PER1 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0126924971145818 0.462445814577084 1133 Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 GPI anchor biosynthetic process|cellular manganese ion homeostasis molecular_function fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum YNL248C RPA49 hom FT NUCLEUS 0.0126282067661663 0.464582989773065 1134 RNA polymerase I subunit A49 transcription elongation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YHR161C YAP1801 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0126081446475759 0.465292837678818 1135 Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YCR033W SNT1 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|NUCLEUS|CHROMOSOME 0.0125921815706604 0.466124192130633 1136 Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance negative regulation of meiosis|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|Set3 complex YLL031C GPI13 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0125912010883969 0.465892812247492 1137 ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity, transferring phosphorus-containing groups integral to membrane|endoplasmic reticulum YBR156C SLI15 hom FT CELL DIVISION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0125539559121573 0.467744882972399 1138 Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly chromosome segregation|protein phosphorylation|regulation of cytokinesis protein kinase activator activity spindle microtubule|spindle midzone|chromosome passenger complex|kinetochore microtubule YDR317W HIM1 hom FT RESPONSE TO DNA DAMAGE 0.0125218621229448 0.468352587883287 1139 Protein of unknown function involved in DNA repair DNA repair molecular_function cellular_component YGL099W LSG1 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|SITE OF POLARIZED GROWTH 0.0124659213774268 0.470342293228339 1140 Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm conjugation with cellular fusion|ribosomal large subunit assembly|ribosomal subunit export from nucleus|ascospore formation GTPase activity mating projection tip|cytoplasm YGL075C MPS2 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0124334506292284 0.471499352458216 1141 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p mitotic anaphase|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton nuclear envelope|spindle pole body YML062C MFT1 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0124276831242536 0.472300389092212 1142 Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance mRNA export from nucleus|DNA recombination|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YLR055C SPT8 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0124089634955448 0.472372961043958 1143 Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters histone acetylation|negative regulation of transcription from RNA polymerase II promoter|chromatin modification|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|transcription cofactor activity nucleus|SAGA complex YOR355W GDS1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0123726756465559 0.47366921182737 1144 Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies aerobic respiration molecular_function mitochondrion|nucleus|cytoplasm YML106W URA5 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0123568072122763 0.474236667431802 1145 Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine ribonucleoside biosynthetic process orotate phosphoribosyltransferase activity nucleus|cytoplasm YML085C TUB1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0123312690072645 0.475348264502764 1146 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|cytoplasmic microtubule|kinetochore microtubule|nuclear microtubule|spindle pole body|polar microtubule YER148W SPT15 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0123236191190695 0.475490501262123 1147 TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability transposon integration|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly RNA polymerase III regulatory region DNA binding|RNA polymerase I transcription factor recruiting transcription factor activity|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding|DNA binding, bending|sequence-specific DNA binding|RNA polymerase II core promoter sequence-specific DNA binding|TFIIIB-type transcription factor activity|chromatin binding|RNA polymerase I transcription factor binding transcription factor TFIIIB complex|RNA polymerase I upstream activating factor complex|nucleus|RNA polymerase I core factor complex|transcription factor TFIID complex YEL036C ANP1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0123232808444261 0.475436797923041 1148 Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol protein N-linked glycosylation alpha-1,6-mannosyltransferase activity|mannosyltransferase activity alpha-1,6-mannosyltransferase complex|Golgi cis cisterna YLR229C CDC42 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0123039050808651 0.476131143293919 1149 Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins budding cell apical bud growth|Rho protein signal transduction|budding cell isotropic bud growth|regulation of initiation of mating projection growth|regulation of vacuole fusion, non-autophagic|regulation of exit from mitosis|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|establishment of cell polarity|invasive growth in response to glucose limitation|positive regulation of exocytosis GTPase activity|signal transducer activity nuclear membrane|fungal-type vacuole membrane|incipient cellular bud site|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YDR213W UPC2 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0122551463976984 0.477880903551691 1150 Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication cellular response to hypoxia|positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|membrane|cytoplasm|perinuclear region of cytoplasm YGR147C NAT2 het FT MITOCHONDRION 0.0122446025022694 0.478259744769366 1151 Protein with an apparent role in acetylation of N-terminal methionine residues N-terminal peptidyl-methionine acetylation molecular_function mitochondrion YOR216C RUD3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|GOLGI APPARATUS 0.0122108496177027 0.479473585390442 1152 Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus YKL104C GFA1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0121979105716069 0.480135856523617 1153 Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication fungal-type cell wall chitin biosynthetic process glutamine-fructose-6-phosphate transaminase (isomerizing) activity cellular_component YHR076W PTC7 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0121558560844458 0.481454888364551 1154 Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope biological_process protein serine/threonine phosphatase activity nuclear envelope|mitochondrion YAL056C-A_d YAL056C-A hom 0.0121548037288996 0.481492845931856 1155 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL237C HAP2 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0121352754601484 0.482393504782172 1156 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex|nucleus YOR048C RAT1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0120726216834503 0.484657583523367 1157 Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination termination of RNA polymerase II transcription, exosome-dependent|nuclear mRNA surveillance|termination of RNA polymerase II transcription, poly(A)-coupled|rRNA processing|RNA processing 5'-3' exoribonuclease activity mitochondrion|nucleus YDL136W RPL35B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0120693215204872 0.484776998725918 1158 Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YDR524C-B_p YDR524C-B hom FT PLASMA MEMBRANE 0.012027774587813 0.486086614718283 1159 Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YNR006W VPS27 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0120110068894625 0.487214950770926 1160 Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) protein retention in Golgi apparatus|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding|phosphatidylinositol-3-phosphate binding endosome|ESCRT-0 complex YKL114C APN1 hom FF|FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0119394118827685 0.489296004881425 1161 Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine base-excision repair 3'-tyrosyl-DNA phosphodiesterase activity|DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity mitochondrion|nucleus YKL218C SRY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0118046543721481 0.494598893969993 1162 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity cellular modified amino acid catabolic process serine racemase activity|threo-3-hydroxyaspartate ammonia-lyase activity cellular_component YGL208W SIP2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PLASMA MEMBRANE 0.0117516038762691 0.496155025592089 1163 One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane cellular response to glucose starvation|signal transduction|protein phosphorylation|replicative cell aging|invasive growth in response to glucose limitation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|plasma membrane|cytoplasm YDR263C DIN7 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0117269479935104 0.497444450211606 1164 Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination DNA repair nuclease activity mitochondrion YLR338W_d OPI9 hom FF 0.0117232800497596 0.497193883877713 1165 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Unknown Unknown Unknown YBR187W_p GDT1 hom 0.0117118030357089 0.497935877325307 1166 Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis biological_process molecular_function integral to membrane|fungal-type vacuole YHR167W THP2 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0117047314900289 0.497874833049404 1167 Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance mRNA export from nucleus|transcription elongation from RNA polymerase II promoter|DNA recombination nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YOR050C_d YOR050C hom 0.0115879434641203 0.502173691380461 1168 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Unknown Unknown Unknown YFR008W FAR7 hom FT CELL CYCLE|SIGNALING 0.0115693564836161 0.503114499800687 1169 Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YKR073C_d YKR073C hom 0.011560404435184 0.503190229605213 1170 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL100W LSB6 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0115464265080513 0.503706607186124 1171 Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity fungal-type vacuole membrane|plasma membrane|cytoplasm YJR088C EMC2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.011520316982707 0.50467190302385 1172 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YNL268W LYP1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.011492465899335 0.505702659495295 1173 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids basic amino acid transport basic amino acid transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YLR170C APS1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0114744449226656 0.506370197773012 1174 Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YCR065W HCM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0114601390207197 0.506963803761704 1175 Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role positive regulation of transcription involved in S-phase of mitotic cell cycle|cellular response to oxidative stress|mitochondrion organization|spindle pole body organization|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nuclear chromatin|nucleus|cytoplasm YEL046C GLY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0114522194212904 0.507384144916082 1176 Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis threonine catabolic process|glycine biosynthetic process threonine aldolase activity|L-allo-threonine aldolase activity cytosol YNR018W RCF2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.011400041471108 0.509131168713388 1177 Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|mitochondrial respiratory chain complex IV|integral to mitochondrial inner membrane YGL026C TRP5 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0113665255772985 0.510377450146887 1178 Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis tryptophan biosynthetic process tryptophan synthase activity nucleus|cytoplasm YOR014W RTS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.0112652958086608 0.514214056573708 1179 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle spindle orientation checkpoint|cellular protein localization|meiotic sister chromatid cohesion, centromeric|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|spindle pole body|condensed nuclear chromosome, centromeric region|cellular bud neck|cytoplasm YHR077C NMD2 hom 0.0112559954331866 0.514498762790156 1180 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|translational frameshifting molecular_function polysome|cytoplasm YBR103W SIF2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0111370553774557 0.518952892105756 1181 WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus negative regulation of meiosis|negative regulation of chromatin silencing at telomere|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|nucleus|Set3 complex YBR004C GPI18 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0110969407808039 0.520459601025078 1182 Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria GPI anchor biosynthetic process mannosyltransferase activity|dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to endoplasmic reticulum membrane|mitochondrion|mannosyltransferase complex|integral to membrane|endoplasmic reticulum YDR392W SPT3 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0110773357792607 0.521196786049382 1183 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters conjugation with cellular fusion|histone acetylation|pseudohyphal growth|ascospore formation|chromatin modification|invasive growth in response to glucose limitation|transcription from RNA polymerase II promoter transcription cofactor activity SLIK (SAGA-like) complex|SAGA complex YHR060W VMA22 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0109885374904725 0.524542484089113 1184 Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) vacuolar proton-transporting V-type ATPase complex assembly|vacuolar acidification unfolded protein binding extrinsic to endoplasmic reticulum membrane YPL183C RTT10 hom FT VESICLE-MEDIATED TRANSPORT 0.0109642864239521 0.52564340739133 1185 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endocytic recycling molecular_function endosome|cytoplasm YGR095C RRP46 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0109530236500579 0.525883628779019 1186 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YPL195W APL5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0109028278972103 0.527782205235508 1187 Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function vesicle-mediated transport|Golgi to vacuole transport protein binding AP-3 adaptor complex YNL195C_p YNL195C hom FT MITOCHONDRION 0.0108862471704669 0.528410112007721 1188 Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YCR093W CDC39 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0108585300186974 0.530075113187084 1189 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex|nucleus|cytoplasm YGL212W VAM7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0108540833179682 0.530059148269708 1190 Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress piecemeal microautophagy of nucleus|vacuole fusion, non-autophagic|vesicle fusion SNAP receptor activity|phosphatidylinositol-3-phosphate binding SNARE complex|fungal-type vacuole membrane YMR071C TVP18 hom FF|FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0107486553060177 0.533818384213317 1191 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|clathrin-coated vesicle YKL110C KTI12 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0107177848105895 0.534993973720817 1192 Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p regulation of transcription from RNA polymerase II promoter|cell growth|tRNA wobble uridine modification chromatin binding nucleus|cytoplasm YDR315C IPK1 hom FT NUCLEUS 0.0106937173435068 0.535728892547643 1193 Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable nuclear-transcribed mRNA catabolic process, non-stop decay|inositol phosphate biosynthetic process inositol pentakisphosphate 2-kinase activity nucleus YDL183C YDL183C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0106884126810296 0.536235410286258 1194 Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene potassium ion transport|proton transport molecular_function integral to mitochondrial inner membrane YML123C PHO84 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0106864222879881 0.536068002630138 1195 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity phosphate ion transport|manganese ion transport|polyphosphate metabolic process manganese ion transmembrane transporter activity|inorganic phosphate transmembrane transporter activity integral to membrane|integral to plasma membrane YGL145W TIP20 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0106808765090686 0.536218830139701 1196 Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p retrograde vesicle-mediated transport, Golgi to ER molecular_function Dsl1p complex|endoplasmic reticulum YOR125C CAT5 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0106396589015681 0.537792995232367 1197 Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation ubiquinone biosynthetic process monooxygenase activity mitochondrion|mitochondrial inner membrane YPL102C_d YPL102C hom 0.0106130566009978 0.538810208916292 1198 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Unknown Unknown Unknown YAL009W SPO7 hom FF|FT CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0105911629251858 0.539708558312891 1199 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation negative regulation of phospholipid biosynthetic process|nuclear envelope organization phosphoprotein phosphatase activity Nem1-Spo7 phosphatase complex|integral to membrane YER129W SAK1 hom FT CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION 0.0105670147247485 0.540633399137973 1200 Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome protein phosphorylation|glucose metabolic process|DNA-dependent DNA replication protein serine/threonine kinase activity|protein kinase activity cytoplasm YML112W CTK3 hom FF|FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0105553684344901 0.541381567128713 1201 Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress mRNA 3'-end processing|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation cyclin-dependent protein kinase activity nucleoplasm|nucleolus|carboxy-terminal domain protein kinase complex YDL111C RRP42 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0105543804321548 0.54105726026331 1202 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) nuclear polyadenylation-dependent tRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YOR254C SEC63 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0105521329311382 0.541143423099434 1203 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation|cytosol to ER transport|SRP-dependent cotranslational protein targeting to membrane|posttranslational protein targeting to membrane protein transporter activity mitochondrion|integral to membrane|Sec62/Sec63 complex|endoplasmic reticulum membrane YBL085W BOI1 hom FT SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0104949901939297 0.543336412930459 1204 Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication cytokinesis, completion of separation|establishment of cell polarity|budding cell apical bud growth|Rho protein signal transduction phospholipid binding site of polarized growth|cellular bud|mating projection tip|cellular bud neck YNR023W SNF12 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RSC COMPLEX 0.0104422024039653 0.545665935947037 1205 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YKR083C DAD2 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0103958573347673 0.547211090038565 1206 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton DASH complex|condensed nuclear chromosome kinetochore|mitotic spindle YBR099C_d YBR099C hom 0.0103203755315048 0.550064953614445 1207 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Unknown Unknown Unknown YCR008W SAT4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0103134054904063 0.550334383914477 1208 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p cellular cation homeostasis|protein phosphorylation|cellular protein localization|G1/S transition of mitotic cell cycle|protein dephosphorylation protein kinase activity cellular_component YNL216W RAP1 het FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0102495612651903 0.552805331185044 1209 Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) negative regulation of chromatin silencing|establishment of protein localization to chromatin|establishment of chromatin silencing at telomere|regulation of transcription by chromatin organization|protection from non-homologous end joining at telomere|negative regulation of transcription from RNA polymerase II promoter|telomere maintenance via telomere lengthening|telomere maintenance|regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter|establishment of protein localization to telomere|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere core promoter proximal region sequence-specific DNA binding|DNA binding, bending|sequence-specific DNA binding|TFIID-class transcription factor binding|TBP-class protein binding RNA polymerase II transcription factor activity|telomeric DNA binding|RNA polymerase II transcription factor binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|G-quadruplex DNA binding|nucleosomal DNA binding nuclear chromosome|nuclear nucleosome|nuclear chromosome, telomeric region|nuclear telomere cap complex YLR051C FCF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0102050312786621 0.554591023730351 1210 Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YOR109W INP53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|CYTOSKELETON 0.0101507026435937 0.55664213067822 1211 Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity membrane|actin cortical patch|cytoplasm YGR158C MTR3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0101235310170044 0.557698956604141 1212 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) nuclear mRNA surveillance|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YLR065C ENV10 hom FF|FT VACUOLAR PROTEIN PROCESSING 0.0100913231198158 0.559246116893494 1213 Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene vacuolar protein processing molecular_function integral to membrane YJR064W CCT5 het 0.0100563364018696 0.56049211394416 1214 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YML072C TCB3 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.0100242186921836 0.56156993778482 1215 Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding mitochondrion|cortical endoplasmic reticulum|cellular bud YDR367W KEI1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00995994193273748 0.564140318317635 1216 Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene inositolphosphoceramide metabolic process inositol phosphoceramide synthase regulator activity integral to membrane|Golgi apparatus|integral to Golgi membrane|inositol phosphoceramide synthase complex|cytoplasm YLR438C-A LSM3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00994645419418122 0.564610068682324 1217 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YOR235W_d IRC13 hom 0.00993836319293648 0.564926832922009 1218 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YDR202C RAV2 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS 0.00989986780081596 0.566435103803147 1219 Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme vacuolar acidification|regulation of protein complex assembly molecular_function RAVE complex YJL094C KHA1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|GOLGI APPARATUS 0.0098971906214832 0.566945342807929 1220 Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies monovalent inorganic cation transport potassium:hydrogen antiporter activity mitochondrion|integral to membrane|Golgi apparatus YNL077W APJ1 hom FT NUCLEUS|MITOCHONDRION 0.00983357964499969 0.569036826580221 1221 Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress biological_process unfolded protein binding mitochondrion|nucleus|cytoplasm YDR443C SSN2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00982493748722599 0.569434016632624 1222 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II transcription coactivator activity|RNA polymerase II transcription factor recruiting transcription factor activity mediator complex YJL014W CCT3 het FF 0.00980139342542045 0.570532149006012 1223 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YDR479C PEX29 hom FT PEROXISOME ORGANIZATION 0.00967152006964021 0.57542136466041 1224 Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress peroxisome organization molecular_function integral to membrane|peroxisomal membrane YGR267C FOL2 het FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00966753948379004 0.575578609579606 1225 GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway folic acid-containing compound biosynthetic process GTP cyclohydrolase I activity nucleus|cytoplasm YMR032W HOF1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00964393038008729 0.57651165581793 1226 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p cytokinesis after mitosis|barrier septum assembly involved in cell cycle cytokinesis|response to DNA damage stimulus|cytokinesis cytoskeletal protein binding cellular bud neck contractile ring|cellular bud neck septin ring YER112W LSM4 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00962201523010132 0.577435155490108 1227 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|cytoplasmic mRNA processing body assembly U6 snRNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YIL060W_p YIL060W hom 0.00953091645525674 0.580987881928242 1228 Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene biological_process molecular_function cellular_component YJL031C BET4 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION 0.00950321421592772 0.582650823921563 1229 Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YGR055W MUP1 hom FT PLASMA MEMBRANE 0.00946750548123098 0.584293321434073 1230 High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake cysteine transport|sulfur amino acid transport|methionine transport L-methionine secondary active transmembrane transporter activity integral to membrane|integral to plasma membrane YDR414C ERD1 hom FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0094659811797606 0.583567154662529 1231 Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) protein retention in ER lumen|protein glycosylation molecular_function integral to membrane|membrane YDR386W MUS81 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS 0.00943064967733814 0.584972782205864 1232 Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p DNA repair|DNA topological change|reciprocal meiotic recombination|resolution of meiotic recombination intermediates|response to DNA damage stimulus crossover junction endodeoxyribonuclease activity|endonuclease activity Holliday junction resolvase complex|nucleus YGR232W NAS6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.00936798898219779 0.588082959712368 1233 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 proteolysis|proteasome regulatory particle assembly molecular_function cytosol|nucleus|proteasome regulatory particle YMR201C RAD14 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0092256648313421 0.593158758817303 1234 Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein nucleotide-excision repair, DNA damage recognition damaged DNA binding|zinc ion binding nucleotide-excision repair factor 1 complex|nucleus YIL043C CBR1 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00916823554739741 0.595461542569198 1235 Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia biological_process cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion YMR156C TPP1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00908768663899817 0.598698220957368 1236 DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase DNA repair polynucleotide 3'-phosphatase activity cellular_component YLR303W MET17 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00903209474767926 0.600936698350325 1237 Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis methionine metabolic process|cysteine biosynthetic process O-acetylhomoserine aminocarboxypropyltransferase activity|cysteine synthase activity plasma membrane|cytoplasm YMR257C PET111 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00903097208479999 0.60098194268547 1238 Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane mitochondrial respiratory chain complex IV biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrion|mitochondrial inner membrane YBL096C_d YBL096C hom 0.00901677998498022 0.601554029763806 1239 Non-essential protein of unknown function Unknown Unknown Unknown YGR195W SKI6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00892303280164133 0.605392949760818 1240 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) nuclear mRNA surveillance|U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|U1 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YBL072C RPS8A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00883960495429782 0.608716568773915 1241 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YNL287W SEC21 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00881525601047101 0.609703866923377 1242 Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport molecular_function endosome|COPI vesicle coat YOL093W TRM10 hom FT RNA PROCESSING|NUCLEUS 0.00879204023373998 0.610645879378809 1243 tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress tRNA methylation tRNA (guanine) methyltransferase activity nucleus|cytoplasm YMR063W RIM9 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00874050533693592 0.613322608912272 1244 Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; ascospore formation molecular_function integral to membrane YDL185W VMA1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM 0.00862094935162333 0.617607932441906 1245 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease intron homing|cellular protein metabolic process|vacuolar acidification endodeoxyribonuclease activity|proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V1 domain|fungal-type vacuole membrane YGL091C NBP35 het FT RNA PROCESSING|COFACTOR METABOLISM|NUCLEUS 0.00861002664260647 0.618053578092699 1246 Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases iron-sulfur cluster assembly|tRNA wobble uridine modification 4 iron, 4 sulfur cluster binding|ATPase activity|iron ion binding mitochondrion|nucleus|cytoplasm YER159C BUR6 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00845825845127494 0.624260188884099 1247 Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity|transcription corepressor activity nucleus|negative cofactor 2 complex YFR002W NIC96 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00839330939044002 0.626924501902242 1248 Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization structural constituent of nuclear pore integral to membrane|nuclear pore nuclear basket|nuclear pore linkers|nuclear pore YHR041C SRB2 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RNA POL II, HOLOENZYME 0.00833189330811143 0.629448369913795 1249 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus|RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|protein domain specific binding core mediator complex YER090W TRP2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00831028100245633 0.631308070933762 1250 Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p tryptophan biosynthetic process anthranilate synthase activity anthranilate synthase complex|cytoplasm YKL109W HAP4 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00830746527773365 0.630453435383195 1251 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex regulation of cellular respiration|carbon catabolite activation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity CCAAT-binding factor complex YHL029C_p OCA5 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.00828012143614801 0.631579280043583 1252 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm YDR453C TSA2 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.00819974759204892 0.634893502269938 1253 Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|cell redox homeostasis peroxiredoxin activity|thioredoxin peroxidase activity cytoplasm YPL176C TRE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.00815611320628846 0.636695843329168 1254 Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function fungal-type vacuole YPL047W SGF11 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.00808901951789743 0.639471377502599 1255 Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation histone deubiquitination|regulation of transcription from RNA polymerase II promoter enzyme activator activity DUBm complex|nucleus|SAGA complex YGL033W HOP2 hom FF|FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.00808781768336709 0.63952114121354 1256 Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair synapsis|reciprocal meiotic recombination protein binding|double-stranded DNA binding condensed nuclear chromosome YOR024W_d YOR024W hom 0.00802172234861432 0.642260406448401 1257 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL077C VAM6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION 0.00799906129721493 0.643200697897304 1258 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle-mediated transport|vacuole organization|vacuole fusion, non-autophagic Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding fungal-type vacuole membrane|HOPS complex|fungal-type vacuole YGL249W ZIP2 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.00789853105430469 0.647526015362638 1259 Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress synaptonemal complex assembly|synapsis|reciprocal meiotic recombination molecular_function synaptonemal complex YBL022C PIM1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.00788294827766794 0.648027599103611 1260 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria chaperone-mediated protein complex assembly|misfolded or incompletely synthesized protein catabolic process ATP-dependent peptidase activity mitochondrion|mitochondrial matrix YGL013C PDR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RSC COMPLEX 0.00787396515663377 0.648401654260338 1261 Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Unknown|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YHR072W ERG7 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.00777815923858487 0.652396492976484 1262 Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis ergosterol biosynthetic process lanosterol synthase activity endoplasmic reticulum|lipid particle|plasma membrane YDL032W_d YDL032W hom 0.00776088184200982 0.653117978339593 1263 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Unknown Unknown Unknown YGR078C PAC10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.00775224552880425 0.654107206883018 1264 Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding tubulin complex assembly tubulin binding prefoldin complex|polysome|cytoplasm YOL120C RPL18A het FF|FT TRANSLATION|RIBOSOME 0.00769827457169645 0.655735103245989 1265 Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YMR113W FOL3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.00764910187060955 0.657793601379483 1266 Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis folic acid-containing compound biosynthetic process dihydrofolate synthase activity cytoplasm YJL183W MNN11 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.00753705262560088 0.662635613312015 1267 Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p protein glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YAL058W CNE1 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00751318586492266 0.663732400978606 1268 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast protein folding|ER-associated protein catabolic process unfolded protein binding integral to endoplasmic reticulum membrane YDR010C_d YDR010C hom 0.00744040939275003 0.667073111222511 1269 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL022W_d YJL022W hom 0.00738921537944939 0.668715946220161 1270 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Unknown Unknown Unknown YJL095W BCK1 hom FF|FT SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH 0.00731425551318493 0.67187950817344 1271 Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p intracellular protein kinase cascade|endoplasmic reticulum unfolded protein response|peroxisome degradation|establishment of cell polarity|protein phosphorylation|response to acid|response to nutrient protein kinase activity|MAP kinase kinase kinase activity intracellular|mating projection tip YGL163C RAD54 hom FF|FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0072501560615821 0.674589341495363 1272 DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress positive regulation of endodeoxyribonuclease activity|DNA geometric change|heteroduplex formation|telomere maintenance via recombination|double-strand break repair via synthesis-dependent strand annealing|chromatin remodeling|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|DNA translocase activity nucleus YGR104C SRB5 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00714511454987243 0.679039139138329 1273 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly core mediator complex YDL126C CDC48 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION|SITE OF POLARIZED GROWTH|UBIQUITI 0.0071367029948582 0.679486256657043 1274 AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP proteasomal ubiquitin-dependent protein catabolic process|mitotic spindle disassembly|ER-associated protein catabolic process|mitochondria-associated protein catabolic process|macroautophagy|sister chromatid biorientation|ER-associated misfolded protein catabolic process|nucleus-associated proteasomal ubiquitin-dependent protein catabolic process|retrograde protein transport, ER to cytosol|piecemeal microautophagy of nucleus|endoplasmic reticulum membrane fusion|ribophagy|Unknown|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process ubiquitin binding|ATPase activity mitochondrion|Cdc48p-Npl4p-Ufd1p AAA ATPase complex|Cdc48p-Npl4p-Vms1p AAA ATPase complex|cytosol|Doa10p ubiquitin ligase complex|nucleus|endoplasmic reticulum membrane|mating projection tip YGL040C HEM2 het FT COFACTOR METABOLISM|NUCLEUS 0.00701771072150504 0.684451285349779 1275 Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus heme biosynthetic process porphobilinogen synthase activity nucleus|cytoplasm YPL062W_d YPL062W hom 0.00700452542360179 0.68519017081772 1276 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Unknown Unknown Unknown YNL246W VPS75 hom FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00693233622441174 0.688087120718093 1277 NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress double-strand break repair via nonhomologous end joining|nucleosome assembly|positive regulation of histone acetylation histone binding|acetyltransferase activator activity chromatin|nucleus YDR455C_d YDR455C hom FF 0.00692664837839858 0.688329605832651 1278 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Unknown Unknown Unknown YER084W_p YER084W hom 0.00692075787416667 0.688624767010048 1279 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YOR072W_d YOR072W hom FF 0.00689820559190475 0.689542663089524 1280 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Unknown Unknown Unknown YGR064W_d YGR064W hom FF 0.0068081711891463 0.693387791553069 1281 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Unknown Unknown Unknown YOR191W ULS1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.00670929113737217 0.697619803360586 1282 Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress chromatin assembly or disassembly|mating type switching SUMO binding|DNA-dependent ATPase activity mitochondrion|nucleolus|nucleus YNR035C ARC35 het FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00661185912555686 0.70201079961763 1283 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin mitochondrion inheritance|receptor-mediated endocytosis|microtubule cytoskeleton organization|actin cytoskeleton organization structural molecule activity cytosol|Arp2/3 protein complex YPL085W SEC16 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00653325125411526 0.705177456205886 1284 COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p COPII vesicle coating protein anchor ER to Golgi transport vesicle membrane YGL148W ARO2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0064712742979586 0.707969908717533 1285 Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress aromatic amino acid family biosynthetic process chorismate synthase activity|riboflavin reductase (NADPH) activity cytoplasm YLR457C NBP1 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.00639217731453585 0.711255073017046 1286 Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication establishment of spindle pole body localization to nuclear envelope|spindle pole body duplication associated with nuclear envelope lipid binding integral to nuclear inner membrane|central plaque of spindle pole body|spindle pole body YER086W ILV1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.00632749484630877 0.714047836454475 1287 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation threonine catabolic process|isoleucine biosynthetic process L-threonine ammonia-lyase activity mitochondrion YLR032W RAD5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00632201076221196 0.714284796879899 1288 DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress double-strand break repair|postreplication repair|protein polyubiquitination|free ubiquitin chain polymerization four-way junction helicase activity|four-way junction DNA binding|DNA-dependent ATPase activity|Y-form DNA binding nuclear chromatin YJR065C ARP3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00623102481627286 0.718220189194275 1289 Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity mitochondrion inheritance|Arp2/3 complex-mediated actin nucleation|actin filament organization ATP binding Arp2/3 protein complex|plasma membrane YMR093W UTP15 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.00599845967473378 0.728741413941982 1290 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|90S preribosome YOL020W TAT2 hom FT PLASMA MEMBRANE 0.00593529507019468 0.731139627472096 1291 High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance aromatic amino acid transport|tryptophan transport high-affinity tryptophan transmembrane transporter activity|aromatic amino acid transmembrane transporter activity integral to membrane|plasma membrane YGR081C SLX9 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00593179046005837 0.731330778905614 1292 Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YOL081W IRA2 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.00592595565906394 0.73150784511881 1293 GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication negative regulation of cAMP biosynthetic process|negative regulation of Ras protein signal transduction|positive regulation of Ras GTPase activity|response to stress Ras GTPase activator activity mitochondrion|integral to membrane|cytoplasm YCR034W FEN1 hom FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00590328606378748 0.732457152465181 1294 Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication fatty acid elongation|vesicle-mediated transport|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum membrane YOR353C SOG2 het FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH 0.00586686109183994 0.734045255077493 1295 Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis signal transduction|cytokinetic cell separation|budding cell apical bud growth molecular_function incipient cellular bud site|cellular bud|mating projection tip|cytoplasm YNL066W SUN4 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0058420989879344 0.735125515197435 1296 Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family mitochondrion organization glucosidase activity fungal-type cell wall|mitochondrial matrix YDR303C RSC3 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.00582804219005018 0.7368415372897 1297 Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p nucleosome positioning|ATP-dependent chromatin remodeling|regulation of cell cycle|nucleosome disassembly|regulation of DNA replication involved in S phase|regulation of transcription, DNA-dependent|G1/S transition of mitotic cell cycle|G2/M transition of mitotic cell cycle|transcription elongation from RNA polymerase II promoter sequence-specific DNA binding|DNA translocase activity|DNA binding RSC complex|nucleus YJR104C SOD1 hom FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.00579751622936014 0.737109535969386 1298 Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid cellular copper ion homeostasis|fungal-type cell wall organization|superoxide metabolic process|cellular zinc ion homeostasis|positive regulation of sequence-specific DNA binding transcription factor activity|age-dependent response to reactive oxygen species involved in chronological cell aging superoxide dismutase activity cytosol|mitochondrion|nucleus|mitochondrial intermembrane space YPL249C GYP5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0057815645535834 0.737919250461625 1299 GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress vesicle-mediated transport|ER to Golgi vesicle-mediated transport Rab GTPase activator activity cytosol|incipient cellular bud site|plasma membrane|cellular bud neck|Golgi-associated vesicle|cellular bud tip YIL026C IRR1 het FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.00574392926447979 0.739600642185376 1300 Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability spore germination|fungal-type cell wall organization|cytogamy|mitotic sister chromatid cohesion chromatin binding nucleus|nuclear mitotic cohesin complex YBR108W AIM3 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00573191075671011 0.73993886215656 1301 Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss actin cortical patch assembly molecular_function membrane raft|actin cortical patch YHR012W VPS29 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.00569663309614149 0.741482058642372 1302 Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval retrograde transport, endosome to Golgi protein transporter activity endosome|retromer complex, inner shell|retromer complex YNL135C FPR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.00565664258704962 0.743343501005031 1303 Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function protein folding|chromatin organization|homoserine metabolic process peptidyl-prolyl cis-trans isomerase activity|macrolide binding mitochondrion|nucleus|cytoplasm YDR357C_p CNL1 hom 0.00562298814167466 0.744743690501533 1304 Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function BLOC-1 complex|cytoplasm YJR063W RPA12 hom FF|FT NUCLEUS 0.00559655536458412 0.745865529183727 1305 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex termination of RNA polymerase I transcription|transcription of nuclear large rRNA transcript from RNA polymerase I promoter molecular_function DNA-directed RNA polymerase I complex YAL036C RBG1 hom FT TRANSLATION|RIBOSOME 0.00558284184237971 0.74646683517331 1306 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation GTP binding polysomal ribosome|polysome|cytoplasm YNL294C RIM21 hom FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00550879408134404 0.749716314928281 1307 pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH fungal-type cell wall biogenesis|ascospore formation|invasive growth in response to glucose limitation molecular_function integral to membrane YDR125C ECM18 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.00549953816576549 0.750122811231245 1308 Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication fungal-type cell wall organization molecular_function mitochondrion YKL165C MCD4 het FF|FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0054868602457379 0.750679705834162 1309 Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes GPI anchor biosynthetic process|ATP transport mannose-ethanolamine phosphotransferase activity integral to membrane|fungal-type cell wall|endoplasmic reticulum|fungal-type vacuole YJL102W MEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.00544285471637843 0.752613713618028 1310 Mitochondrial elongation factor involved in translational elongation translational elongation translation elongation factor activity mitochondrion YDR076W RAD55 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00543285777900258 0.753053287948885 1311 Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p DNA recombinase assembly|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YML097C VPS9 hom FF|FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.00540311285078868 0.754361672036237 1312 A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin vacuole inheritance|protein targeting to vacuole ubiquitin binding|guanyl-nucleotide exchange factor activity cytosol YLR180W SAM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.00540021111502878 0.754489347896977 1313 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cytoplasm YLR110C CCW12 hom FF|FT CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH 0.0053582546972494 0.756441732750275 1314 Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication conjugation with cellular fusion|agglutination involved in conjugation with cellular fusion|fungal-type cell wall organization molecular_function fungal-type cell wall|mating projection tip|cellular bud tip YKL195W MIA40 het FF|FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00526153116554267 0.760772088034374 1315 Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial outer membrane translocase complex|mitochondrial intermembrane space YDR067C_p OCA6 hom 0.00524094403143254 0.761507267804524 1316 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm YJL024C APS3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00522832860410296 0.762063999602356 1317 Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YML034C-A_d YML034C-A hom 0.0051611320877818 0.765031518718066 1318 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR198W SGE1 hom FF|FT PLASMA MEMBRANE 0.00515843136195128 0.765320860699478 1319 Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations response to drug|drug transport drug transmembrane transporter activity integral to membrane|integral to plasma membrane YGR203W YCH1 hom FT NUCLEUS 0.00510386637330312 0.767563201682723 1320 Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus dephosphorylation phosphatase activity|protein tyrosine phosphatase activity|thiosulfate sulfurtransferase activity nucleus|cytoplasm YGL168W HUR1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS 0.00509843619869044 0.767803395664973 1321 Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene DNA replication molecular_function cellular_component YGL225W VRG4 het FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.00495489298415729 0.774259085623236 1322 Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi GDP-mannose transport GDP-mannose transmembrane transporter activity mitochondrion|integral to membrane|Golgi apparatus YLR039C RIC1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.00489866513056166 0.776655223830038 1323 Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes intracellular protein transport|retrograde transport, endosome to Golgi guanyl-nucleotide exchange factor activity nucleus|Ric1p-Rgp1p complex|Golgi membrane|guanyl-nucleotide exchange factor complex YKR026C GCN3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.00489598826953754 0.776774035078036 1324 Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YOR030W DFG16 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.00485482791503802 0.778762386174526 1325 Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p pseudohyphal growth|protein processing molecular_function integral to membrane YBR069C TAT1 hom FF|FT PLASMA MEMBRANE 0.00482303683498405 0.780493862086686 1326 Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress amino acid transport|tyrosine transport|tryptophan transport|transmembrane transport amino acid transmembrane transporter activity|low-affinity tryptophan transmembrane transporter activity integral to membrane|plasma membrane YOR067C ALG8 hom FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00478089397585364 0.781887344627351 1327 Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p oligosaccharide-lipid intermediate biosynthetic process|protein N-linked glycosylation dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YLR038C COX12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0047053644101482 0.785248050009317 1328 Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV mitochondrial respiratory chain complex IV assembly cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDR495C VPS3 hom FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.00469113123244998 0.785881808912043 1329 Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase vacuole inheritance|vacuolar acidification|protein targeting to vacuole molecular_function CORVET complex|cytoplasm YPR100W MRPL51 hom FT TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION|RIBOSOME 0.00463735196693482 0.788370178293462 1330 Mitochondrial ribosomal protein of the large subunit mitochondrial translation|aerobic respiration structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YPR062W FCY1 hom FT NUCLEUS 0.00462194165722487 0.788964618015842 1331 Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) cytosine metabolic process|pyrimidine-containing compound salvage|cytidine metabolic process cytosine deaminase activity nucleus|cytoplasm YKL135C APL2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.00447740856855218 0.795415044568776 1332 Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YOL150C_d YOL150C hom 0.00447020224697955 0.795737028565421 1333 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL125W KAP120 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00445014181488316 0.796633527070551 1334 Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p protein import into nucleus|transcription factor import into nucleus protein transporter activity|structural constituent of nuclear pore integral to membrane|nucleus|nuclear pore|cytoplasm YNL231C PDR16 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00442419410509106 0.797911541390013 1335 Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress response to drug|sterol biosynthetic process|phospholipid biosynthetic process|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cell periphery|lipid particle|plasma membrane|cytoplasm YIL154C IMP2' hom FF|FT RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS 0.00439721824194219 0.799293458897456 1336 Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat DNA repair|cellular carbohydrate metabolic process transcription coactivator activity cytoplasm YNL295W_p YNL295W hom 0.00435295205788445 0.801358747190574 1337 Putative protein of unknown function biological_process molecular_function cellular_component YBR055C PRP6 het FT RNA PROCESSING|NUCLEUS 0.00431510168980961 0.802934887375109 1338 Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YOR234C RPL33B hom FT TRANSLATION|RIBOSOME 0.00429317097988109 0.803657711591115 1339 Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR399C BDF1 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00423719762646339 0.80619462381177 1340 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p DNA repair|snRNA transcription|regulation of chromatin silencing at telomere|regulation of transcription by chromatin organization|regulation of chromatin silencing at silent mating-type cassette|Unknown|chromatin remodeling|negative regulation of heterochromatin assembly histone acetyl-lysine binding|chromatin binding|core promoter binding|TFIID-class transcription factor binding nuclear chromatin|Swr1 complex YDR372C VPS74 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.00415846917370255 0.809837281037609 1341 Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 retrograde vesicle-mediated transport, Golgi to ER|endoplasmic reticulum unfolded protein response|protein localization to Golgi apparatus enzyme binding|phosphatidylinositol-4-phosphate binding cytosol|Golgi apparatus|nucleus|extrinsic to membrane|cytoplasm YPL042C SSN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00406481131689127 0.813986377054 1342 Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression phosphorylation of RNA polymerase II C-terminal domain|negative regulation of transcription from RNA polymerase II promoter|negative regulation of filamentous growth|protein phosphorylation|protein destabilization|nuclear-transcribed mRNA catabolic process, non-stop decay|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II carboxy-terminal domain kinase activity|protein kinase activity|cyclin-dependent protein kinase activity mediator complex YNR012W URK1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.00393821108232888 0.819599305505458 1343 Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP pyrimidine-containing compound salvage uridine kinase activity nucleus|cytoplasm YMR073C IRC21 hom FF 0.00391506614151503 0.820851864179333 1344 Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study response to drug molecular_function cytoplasm YDL196W_d YDL196W het FF 0.00390754288835782 0.8210589848193 1345 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Unknown Unknown Unknown YJL185C ATG36 hom 0.00389122036956242 0.821715454860352 1346 Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene peroxisome degradation molecular_function peroxisome YNL130C-A_p DGR1 hom FT MITOCHONDRION 0.0038245300885242 0.824720965583222 1347 Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose biological_process molecular_function cellular_component YLR396C VPS33 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.00382300834197597 0.824789575835361 1348 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole regulation of SNARE complex assembly|endocytosis|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle fusion with vacuole|Golgi to vacuole transport|vacuole organization|vacuole fusion, non-autophagic|vesicle docking|protein targeting to vacuole phosphatidylinositol binding|ATP binding cytosol|fungal-type vacuole membrane|HOPS complex|fungal-type vacuole|CORVET complex YNL240C NAR1 het FT COFACTOR METABOLISM|NUCLEUS 0.00379430731085985 0.826083855400326 1349 Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf iron-sulfur cluster assembly iron-sulfur cluster binding|ferredoxin hydrogenase activity cytosol|membrane YCR081W SRB8 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00364407504210012 0.832939917843305 1350 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription by galactose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity mediator complex YOR070C GYP1 hom FF|FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.00357063051211056 0.836258790336168 1351 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion vesicle-mediated transport Rab GTPase activator activity mitochondrion|Golgi apparatus YBR013C_p YBR013C hom 0.00349343465511038 0.839679569345446 1352 Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YML075C HMG1 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00347678055266759 0.840550968301798 1353 HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress ergosterol biosynthetic process|isopentenyl diphosphate biosynthetic process, mevalonate pathway hydroxymethylglutaryl-CoA reductase (NADPH) activity nuclear envelope|integral to membrane|endoplasmic reticulum membrane YER095W RAD51 hom FF|FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00344746377417302 0.841761207787339 1354 Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein strand invasion|telomere maintenance via recombination|heteroduplex formation|meiotic joint molecule formation|reciprocal meiotic recombination|double-strand break repair via single-strand annealing recombinase activity|single-stranded DNA binding|DNA-dependent ATPase activity condensed nuclear chromosome|nuclear chromosome YDR540C IRC4 hom FF|FT NUCLEUS 0.00342659284727162 0.84270664424072 1355 Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus mitotic recombination molecular_function nucleus|cytoplasm YOR236W DFR1 het FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.00342450132285758 0.842801401157409 1356 Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism tetrahydrofolate biosynthetic process|dihydrofolate metabolic process dihydrofolate reductase activity|mRNA binding cytosol YOR303W CPA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00321098187398075 0.852486675019442 1357 Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader arginine biosynthetic process carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity carbamoyl-phosphate synthase complex YMR031W-A_d YMR031W-A hom 0.00316652530503481 0.854613377246005 1358 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Unknown Unknown Unknown YER131W RPS26B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.00316168159176659 0.854726161861032 1359 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YBR086C IST2 hom FT PLASMA MEMBRANE 0.00314695586400235 0.855395312281521 1360 Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process response to osmotic stress lipid binding integral to membrane|cellular bud membrane|plasma membrane YGL043W DST1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS 0.00312048383125455 0.856598486538575 1361 General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress transcription elongation from RNA polymerase I promoter|transcription initiation from RNA polymerase II promoter|mRNA cleavage|maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter|tRNA transcription from RNA polymerase III promoter|regulation of mRNA 3'-end processing|positive regulation of transcription elongation from RNA polymerase II promoter|transcription from RNA polymerase III promoter|transcription antitermination|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter RNA polymerase II regulatory region DNA binding|RNA polymerase II core binding|core RNA polymerase II recruiting transcription factor activity nucleus YBR289W SNF5 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.00311581589692849 0.856810682380922 1362 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|double-strand break repair via homologous recombination|chromatin remodeling|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding SWI/SNF complex|nucleus YJL197W UBP12 hom FT PROTEOLYSIS|NUCLEUS 0.00311089418325649 0.857034425777011 1363 Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm biological_process ubiquitin-specific protease activity nucleus|cytoplasm YGL158W RCK1 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION 0.00305307683602519 0.859663684410628 1364 Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication protein phosphorylation|regulation of meiosis protein serine/threonine kinase activity|protein kinase activity cellular_component YDR477W SNF1 hom FT KETONE METABOLISM|SIGNALING|TRANSLATION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00294273381900731 0.864685856321669 1365 AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation negative regulation of translation|protein phosphorylation|replicative cell aging|signal transduction|biofilm formation|positive regulation of gluconeogenesis|pseudohyphal growth|cell adhesion|regulation of carbohydrate metabolic process|invasive growth in response to glucose limitation|cellular response to nitrogen starvation AMP-activated protein kinase activity|protein kinase activity mitochondrion|AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|fungal-type vacuole|cytoplasm YBR027C_d YBR027C hom 0.00292611674720246 0.865442647440456 1366 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR319C FET4 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00287899923402191 0.86772618143871 1367 Low-affinity Fe(II) transporter of the plasma membrane intracellular copper ion transport|zinc ion transport|low-affinity iron ion transport|copper ion import copper ion transmembrane transporter activity|iron ion transmembrane transporter activity integral to membrane|plasma membrane|integral to plasma membrane YNL112W DBP2 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.00278309732733322 0.872055871939488 1368 ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|rRNA processing RNA helicase activity mitochondrion|nucleus|cytoplasm YML090W_d YML090W hom 0.00273086049458231 0.874344258646834 1369 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Unknown Unknown Unknown YAR042W SWH1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00267823665555013 0.876746117282295 1370 Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction endocytosis|maintenance of cell polarity|exocytosis|sterol transport lipid binding|oxysterol binding|1-phosphatidylinositol binding|sterol transporter activity nuclear envelope|endoplasmic reticulum|Golgi trans cisterna|early endosome YDR229W IVY1 hom FT MEMBRANE ORGANIZATION 0.00249390329915026 0.885168272676194 1371 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase vacuole fusion, non-autophagic phospholipid binding fungal-type vacuole membrane YER168C CCA1 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.00248520615397904 0.885565971787351 1372 ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites tRNA 3'-terminal CCA addition tRNA adenylyltransferase activity mitochondrion|nucleus|mitochondrial matrix|cytoplasm YMR138W CIN4 hom FT SIGNALING 0.00244427529814288 0.887438024676991 1373 GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog protein folding|tubulin complex assembly|tubulin complex biogenesis GTP binding cytoplasm YER022W SRB4 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00242731397888833 0.888213968447817 1374 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination transcription initiation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter activating transcription factor binding|structural molecule activity|core RNA polymerase II recruiting transcription factor activity core mediator complex YER149C PEA2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00232202946977578 0.893032874673644 1375 Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth bipolar cellular bud site selection|regulation of initiation of mating projection growth|regulation of termination of mating projection growth|actin filament organization|pseudohyphal growth|establishment of cell polarity|filamentous growth|Rho protein signal transduction cytoskeletal regulatory protein binding polarisome|actin cap|mating projection tip YHR143W-A RPC10 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00231564853030374 0.893325061090973 1376 RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YBL058W SHP1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.00229566241281151 0.894318738036153 1377 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate glycogen metabolic process|proteasomal ubiquitin-dependent protein catabolic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|ascospore formation|sister chromatid biorientation|autophagic vacuole assembly protein phosphatase type 1 regulator activity nucleus|cytoplasm YPR123C_d YPR123C hom FF 0.00229246827955353 0.894433591924789 1378 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Unknown Unknown Unknown YBL045C COR1 hom FT OXIDATION-REDUCTION PROCESS|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00228971139424942 0.894512884113169 1379 Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YHR013C ARD1 hom FT CHROMATIN ORGANIZATION|RIBOSOME 0.0022822683425483 0.894853791597444 1380 Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress histone acetylation|N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity cytosolic ribosome|NatA complex YBR227C MCX1 hom FT MITOCHONDRION 0.00220922399811384 0.898200397151093 1381 Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein refolding molecular_function mitochondrion|mitochondrial matrix YNL064C YDJ1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION 0.0021877524712864 0.89918448535004 1382 Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family protein refolding|ER-associated protein catabolic process|protein targeting to mitochondrion|protein targeting to ER|'de novo' protein folding unfolded protein binding|ATPase activator activity cytosol|TRC complex|perinuclear region of cytoplasm YMR068W AVO2 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.00217793442706513 0.899634520042468 1383 Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|cytoplasm YPR193C HPA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION 0.00212317191120907 0.902159802781538 1384 Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity protein homotetramerization|histone acetylation histone acetyltransferase activity|protein homodimerization activity cytoplasm YNL297C MON2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00205798517706943 0.905149334505267 1385 Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins transport|endocytosis|protein targeting to vacuole|Golgi to endosome transport guanyl-nucleotide exchange factor activity cytosol|endosome|trans-Golgi network|extrinsic to membrane YNL243W SLA2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00200658175039244 0.907493967850973 1386 Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo bipolar cellular bud site selection|fungal-type cell wall organization|actin filament organization|endocytosis|exocytosis protein binding, bridging incipient cellular bud site|mating projection tip|actin cortical patch YOR246C ENV9 hom FF|FT OXIDATION-REDUCTION PROCESS|VACUOLAR PROTEIN PROCESSING 0.00193155618331836 0.910990980547407 1387 Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts vacuole organization|vacuolar protein processing oxidoreductase activity lipid particle YIL064W SEE1 hom FT VESICLE-MEDIATED TRANSPORT 0.00186878086719988 0.913821104036594 1388 Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport vesicle-mediated transport|peptidyl-lysine dimethylation protein-lysine N-methyltransferase activity cytoplasm YLR240W VPS34 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|GOLGI APPARATUS 0.0017803167355913 0.917885845015755 1389 Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery peroxisome degradation|phosphatidylinositol phosphorylation|protein phosphorylation 1-phosphatidylinositol-3-kinase activity|protein kinase activity endosome|fungal-type vacuole membrane|phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|peroxisome|pre-autophagosomal structure YPL183W-A RTC6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00174893199076204 0.919328429382069 1390 Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress mitochondrial translation|ribosome biogenesis structural constituent of ribosome mitochondrial large ribosomal subunit YLR233C EST1 hom FT NUCLEUS|CHROMOSOME 0.00170271366130935 0.921453314105958 1391 TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA telomere maintenance via telomerase|telomere maintenance|G-quadruplex DNA formation single-stranded DNA binding|RNA binding|telomeric DNA binding nucleolus|nucleus|telomerase holoenzyme complex YDL226C GCS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|CYTOSKELETON 0.00166020774340115 0.923408016169125 1392 ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p retrograde vesicle-mediated transport, Golgi to ER|Golgi to plasma membrane protein transport|actin filament reorganization involved in cell cycle|ER to Golgi vesicle-mediated transport actin binding|ARF GTPase activator activity|GTPase activator activity endosome|trans-Golgi network|endoplasmic reticulum-Golgi intermediate compartment|cytoskeleton YDR160W SSY1 het FT PLASMA MEMBRANE 0.0016489333117817 0.923926566869955 1393 Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes response to amino acid stimulus molecular_function integral to membrane|plasma membrane YNR008W LRO1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.00159784090458039 0.926276887899588 1394 Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase lipid storage|triglyceride biosynthetic process phospholipid:diacylglycerol acyltransferase activity lipid particle|perinuclear endoplasmic reticulum YGR036C CAX4 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0015635199876723 0.927856059732286 1395 Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation lipid biosynthetic process|protein N-linked glycosylation dolichyldiphosphatase activity integral to endoplasmic reticulum membrane|integral to membrane YGR228W_d YGR228W hom 0.00153535115065394 0.929152374993677 1396 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Unknown Unknown Unknown YKL033W TTI1 het FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.00153049988994879 0.929386149752541 1397 Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ASTRA complex|mitochondrion|cytoplasm YBL036C YBL036C hom 0.00151374431389097 0.930146837957832 1398 Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process pyridoxal phosphate binding cellular_component YML034W SRC1 hom FF|FT CELL CYCLE|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.00151092452259257 0.930318116924856 1399 Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance maintenance of rDNA|mitotic sister chromatid segregation molecular_function nuclear envelope YER082C UTP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00149228649568615 0.931144793130313 1400 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YOR012W_p YOR012W hom 0.00148291331087147 0.931606760595356 1401 Putative protein of unknown function biological_process molecular_function cellular_component YMR021C MAC1 hom FF|FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 0.00148106740561821 0.931651011853482 1402 Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport cellular copper ion homeostasis|positive regulation of protein catabolic process|positive regulation of transcription from RNA polymerase II promoter core promoter proximal region DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YIL128W MET18 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00145997712108954 0.932652036402831 1403 Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 methionine metabolic process|nucleotide-excision repair|transcription from RNA polymerase II promoter molecular_function cytoplasm YBR189W RPS9B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.00145225541351834 0.932977479679052 1404 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication positive regulation of translational fidelity|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YNL220W ADE12 hom FT NUCLEOTIDE METABOLISM 0.00141248660833765 0.934808690259298 1405 Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence purine nucleotide biosynthetic process DNA replication origin binding|adenylosuccinate synthase activity cytoplasm YGR155W CYS4 hom FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|MITOCHONDRION 0.0013977654453898 0.935573073690398 1406 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria cysteine biosynthetic process from serine|hydrogen sulfide biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|traversing start control point of mitotic cell cycle cystathionine beta-synthase activity mitochondrion|cytoplasm YEL049W PAU2 hom 0.00134672910210118 0.937837339017704 1407 Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YDR382W RPP2B hom FT TRANSLATION|RIBOSOME 0.00133674484555297 0.938297272023257 1408 Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR152C_p YOR152C hom 0.00133583044763997 0.93833939554749 1409 Putative protein of unknown function; YOR152C is not an essential gene biological_process molecular_function membrane YOL141W PPM2 hom FT RNA PROCESSING|MITOCHONDRION 0.00131930153048061 0.939100861992564 1410 AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA methylation|wybutosine biosynthetic process tRNA methyltransferase activity mitochondrion|cytoplasm YMR135W-A_d YMR135W-A hom 0.00131777735793268 0.939225417230704 1411 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFL031W HAC1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|NUCLEUS 0.00129146207761279 0.940383516076008 1412 Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress endoplasmic reticulum unfolded protein response|positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response|negative regulation of transcription from RNA polymerase II promoter during meiosis|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YJR098C_p YJR098C hom FT LIPID METABOLISM|MITOCHONDRION 0.00128318029856926 0.940765114539956 1413 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YIL158W AIM20 hom FT CELL CYCLE 0.0012758329952311 0.941103666669475 1414 Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process molecular_function fungal-type vacuole YDL109C_p YDL109C hom FT LIPID METABOLISM 0.0012457333951748 0.942490719085778 1415 Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication cellular lipid metabolic process molecular_function cellular_component YER060W-A FCY22 hom 0.00124019365644568 0.942814236125693 1416 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YGL146C_p RRT6 hom 0.00117815342472359 0.945605574044554 1417 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins biological_process molecular_function membrane YDL235C YPD1 het FT SIGNALING|NUCLEUS 0.00114829172135213 0.946982209213758 1418 Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus osmosensory signaling pathway via two-component system protein histidine kinase binding|transferase activity, transferring phosphorus-containing groups nucleus|cytoplasm YAL066W_d YAL066W hom 0.00114819204935482 0.947042081006916 1419 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR262C BUD32 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|LIPID METABOLISM|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.0011276143507761 0.947935536334687 1420 Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription threonylcarbamoyladenosine metabolic process|protein phosphorylation|telomere maintenance|cellular bud site selection|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity EKC/KEOPS complex|nucleus|cytoplasm YDR048C_d YDR048C hom 0.00107929660688246 0.950163506912351 1421 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFR003C YPI1 het FT CELL CYCLE|LIPID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00105979514153882 0.951062846687801 1422 Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance negative regulation of phosphoprotein phosphatase activity|glycogen metabolic process|cellular ion homeostasis|Unknown|positive regulation of phosphoprotein phosphatase activity|negative regulation of protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint protein serine/threonine phosphatase inhibitor activity|protein phosphatase type 1 activator activity nucleus YKR099W BAS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00104477152023549 0.952508804739638 1423 Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes positive regulation of transcription from RNA polymerase II promoter core promoter sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity nucleus YJL175W_d YJL175W hom 0.0010337031191769 0.952287547611738 1424 Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Unknown Unknown Unknown YBR200W BEM1 hom FT SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00102602612075061 0.952620303211305 1425 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p establishment of cell polarity|cell morphogenesis involved in conjugation with cellular fusion protein complex scaffold|phosphatidylinositol-3-phosphate binding incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip YAL061W_p BDH2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00102109875878459 0.95284757268251 1426 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor nucleus|cytoplasm YDR107C TMN2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.00101231861783401 0.953252557085303 1427 Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments cellular copper ion homeostasis|pseudohyphal growth|vacuolar transport|invasive growth in response to glucose limitation molecular_function fungal-type vacuole membrane|integral to membrane YPL041C_p YPL041C hom 0.00100343464627829 0.953710675821458 1428 Protein of unknown function involved in maintenance of proper telomere length biological_process molecular_function cellular_component YJL117W PHO86 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.000984812554663353 0.954521353136375 1429 Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress protein folding|ER to Golgi vesicle-mediated transport|regulation of phosphate transport unfolded protein binding endoplasmic reticulum YCR021C HSP30 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.000969669330746296 0.955219927150026 1430 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase negative regulation of ATPase activity|response to stress molecular_function integral to membrane|plasma membrane YDL208W NHP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.000964976809770101 0.955436405443207 1431 Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing cleavage involved in rRNA processing|rRNA pseudouridine synthesis|snRNA pseudouridine synthesis snoRNA binding box H/ACA snoRNP complex YLR084C RAX2 hom FT CELL CYCLE|CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.000964654609226378 0.955457905234117 1432 N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cellular bud site selection molecular_function mitochondrion|cellular bud scar|cellular bud neck|membrane YPR180W AOS1 het FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0009641573822252 0.955607043264915 1433 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity nucleus|SUMO activating enzyme complex YFR039C_p YFR039C hom 0.000959481773912763 0.955689910027136 1434 Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component YER133W GLC7 het FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CELL DIVISION|CARBOHYDRATE METABOLISM 0.000952827777399451 0.956016554147966 1435 Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest replication fork processing|glycogen metabolic process|chromosome segregation|regulation of cell shape during vegetative growth phase|termination of RNA polymerase II transcription, poly(A)-coupled|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|rRNA processing|regulation of cell cycle|ascospore formation|protein localization to kinetochore|cell budding|DNA damage checkpoint|meiosis|termination of RNA polymerase II transcription, exosome-dependent|DNA replication checkpoint|response to heat|cellular ion homeostasis|histone dephosphorylation|regulation of carbohydrate metabolic process protein serine/threonine phosphatase activity protein phosphatase type 1 complex|mating projection base|condensed nuclear chromosome kinetochore|mRNA cleavage and polyadenylation specificity factor complex|nucleolus|spindle pole body|cellular bud neck YOR210W RPB10 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.000946281030781865 0.956298923202609 1436 RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase II, core complex YGR018C_p YGR018C hom 0.000929454105472742 0.95707526636255 1437 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component YLR390W-A CCW14 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.000921106664440984 0.957485760851737 1438 Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall fungal-type cell wall organization structural constituent of cell wall mitochondrion|fungal-type cell wall YIL042C PKP1 hom FT SIGNALING|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.000903145255859753 0.958314018729072 1439 Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p peptidyl-serine phosphorylation|protein phosphorylation|carbon utilization protein kinase activity|pyruvate dehydrogenase (acetyl-transferring) kinase activity mitochondrion YGL119W COQ8 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.000902792803198857 0.958336486996657 1440 Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication ubiquinone biosynthetic process|aerobic respiration molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial matrix YGR097W ASK10 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|RNA POL II, HOLOENZYME 0.000900071249770291 0.958430999114407 1441 Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication cellular protein catabolic process|cellular response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter|positive regulation of glycerol transport molecular_function DNA-directed RNA polymerase II, holoenzyme|cytoplasm YOL154W ZPS1 hom 0.000882947755057802 0.959221137382388 1442 Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH biological_process molecular_function fungal-type cell wall|fungal-type vacuole YJR137C MET5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS 0.000879506880554532 0.959410183287137 1443 Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine fungal-type cell wall organization|sulfate assimilation sulfite reductase (NADPH) activity sulfite reductase complex (NADPH)|cytoplasm YGL149W_d YGL149W hom 0.000872036790685223 0.959724628587874 1444 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Unknown Unknown Unknown YCL023C_d YCL023C hom 0.000855086740770142 0.960506827804187 1445 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Unknown Unknown Unknown YMR074C YMR074C hom FT NUCLEUS 0.000850705237860622 0.960709028590995 1446 Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress apoptotic process molecular_function nucleus|cytoplasm YKL220C FRE2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.000849858016227922 0.960748127083717 1447 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YLR209C PNP1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.000849396201004574 0.960769439467777 1448 Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway guanosine catabolic process|inosine catabolic process|NAD biosynthesis via nicotinamide riboside salvage pathway|nicotinate nucleotide salvage nicotinamide riboside hydrolase activity|inosine nucleosidase activity|purine-nucleoside phosphorylase activity intracellular YGR251W NOP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.000834252485689963 0.961468324626828 1449 Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|nucleolus|nucleus|90S preribosome YOR087W YVC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.000823522566109658 0.961963530272759 1450 Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular cation homeostasis voltage-gated ion channel activity|calcium activated cation channel activity|sodium channel activity|calcium channel activity|potassium channel activity fungal-type vacuole membrane|fungal-type vacuole YGR022C_d YGR022C hom 0.000816512532282509 0.962287064240577 1451 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Unknown Unknown Unknown YDR517W GRH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000802583155437592 0.962929964894979 1452 Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress protein secretion|ER to Golgi vesicle-mediated transport|Golgi organization molecular_function COPII vesicle coat|cis-Golgi network|cytoplasm YBR090C_p YBR090C hom FT NUCLEUS 0.000798236281478463 0.963169069574972 1453 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YDR139C RUB1 hom 0.000789144016910763 0.963550261782359 1454 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein neddylation protein tag cytoplasm YCR071C IMG2 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.000786122022872322 0.963722222826504 1455 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YIL114C POR2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.000784176469827448 0.96377954949092 1456 Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication DNA transport|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to membrane YDR256C CTA1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.000783946362491498 0.963790170657472 1457 Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation response to reactive oxygen species|age-dependent response to reactive oxygen species|hydrogen peroxide catabolic process catalase activity peroxisomal matrix|mitochondrial matrix YNL263C YIF1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.000783763684461987 0.963798602613025 1458 Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 ER to Golgi vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|endoplasmic reticulum membrane|ER to Golgi transport vesicle YER044C-A MEI4 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.000776881764694399 0.964116257553382 1459 Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic DNA double-strand break formation molecular_function condensed nuclear chromosome YGR023W MTL1 hom FT CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|PLASMA MEMBRANE 0.000751710566993384 0.96531956945712 1460 Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p cellular response to glucose starvation|fungal-type cell wall organization|cellular response to oxidative stress molecular_function integral to plasma membrane YBL086C_p YBL086C hom 0.000741545456507456 0.965747395777193 1461 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YGR187C HGH1 hom 0.000736122653647894 0.966002794370882 1462 Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function cytoplasm YJL171C YJL171C hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0007310688239163 0.966231029202664 1463 GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion|fungal-type cell wall|plasma membrane YML108W YML108W hom FT NUCLEUS 0.000728471769010254 0.96635091913312 1464 Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL081W_p YGL081W hom 0.000719870253057672 0.966748003233252 1465 Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis biological_process molecular_function cellular_component YDR465C RMT2 hom FT NUCLEUS 0.000709757720766313 0.967273531062255 1466 Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress peptidyl-arginine methylation protein-arginine N5-methyltransferase activity nucleus|cytoplasm YNR032W PPG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.000708588343982826 0.96726883863887 1467 Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases glycogen metabolic process|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YNL218W MGS1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.000700866214368426 0.967625342968784 1468 Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress regulation of DNA repair|DNA replication, Okazaki fragment processing|DNA strand renaturation ubiquitin binding|single-stranded DNA-dependent ATPase activity|enzyme activator activity nucleus|cytoplasm YGR209C TRX2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.000699173403985295 0.967703495104895 1469 Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress vacuole inheritance|regulation of DNA replication|cell redox homeostasis|protein deglutathionylation|sulfate assimilation|retrograde vesicle-mediated transport, Golgi to ER|cellular response to oxidative stress|ER to Golgi vesicle-mediated transport|vacuole fusion, non-autophagic disulfide oxidoreductase activity cytosol|fungal-type vacuole YBR118W TEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|CYTOSKELETON|RIBOSOME 0.000696845661961772 0.967810960695895 1470 Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication translational elongation|tRNA export from nucleus GDP binding|GTP binding|translation elongation factor activity ribosome|eukaryotic translation elongation factor 1 complex|cytoplasm YBR097W VPS15 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|PROTEIN PHOSPHORYLATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.000690188774960418 0.96811829448969 1471 Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery protein retention in Golgi apparatus|vacuole inheritance|peroxisome degradation|protein phosphorylation|macroautophagy|inositol lipid-mediated signaling|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding|protein serine/threonine kinase activity mitochondrion|phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|Golgi membrane YNL142W MEP2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.000681987304370767 0.968515728814818 1472 Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation nitrogen utilization|ammonium transport|pseudohyphal growth ammonium transmembrane transporter activity integral to membrane|plasma membrane YKL113C RAD27 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.000665633336740832 0.96925200550355 1473 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family double-strand break repair via nonhomologous end joining|replicative cell aging|DNA replication, removal of RNA primer|base-excision repair, base-free sugar-phosphate removal|gene conversion at mating-type locus, DNA repair synthesis 5'-3' exonuclease activity|5'-flap endonuclease activity mitochondrion|nucleus YMR008C PLB1 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.000665322499039025 0.969266357111433 1474 Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol glycerophospholipid metabolic process lysophospholipase activity periplasmic space|plasma membrane YDL059C RAD59 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00066046064775172 0.969504476659574 1475 Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p DNA strand renaturation|telomere maintenance via recombination|double-strand break repair via break-induced replication|double-strand break repair via single-strand annealing protein binding nucleus YDR242W AMD2 hom 0.000659711607354325 0.969584514612599 1476 Putative amidase biological_process amidase activity cellular_component YHR183W GND1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.000647074662717216 0.970131171974133 1477 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication cellular response to oxidative stress|pentose-phosphate shunt, oxidative branch phosphogluconate dehydrogenase (decarboxylating) activity mitochondrion|cytoplasm YDL157C_p YDL157C hom FT MITOCHONDRION 0.000641831226362037 0.970350995096064 1478 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDR023W SES1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.000635621670350932 0.970637711679179 1479 Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p seryl-tRNA aminoacylation serine-tRNA ligase activity cytoplasm YLR211C_p YLR211C hom 0.000631936933873299 0.970807850446127 1480 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron biological_process molecular_function cytoplasm YGL114W_p YGL114W hom FT PROTEIN LOCALIZATION 0.00063063621109928 0.970867910246946 1481 Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters transport oligopeptide transporter activity integral to membrane|membrane YFL009W CDC4 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.000624229434391017 0.971168038154205 1482 F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p meiosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity|ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus|nuclear matrix YKR023W_p YKR023W hom FT NUCLEUS|MITOCHONDRION 0.000622426972556846 0.971246968859108 1483 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YLR200W YKE2 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.000620843178569498 0.971320100721782 1484 Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding|tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YBL015W ACH1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.000617337629021222 0.971481970716518 1485 Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth acetate metabolic process acetate CoA-transferase activity|acetyl-CoA hydrolase activity cytosol|mitochondrion YLR346C_p YLR346C hom FT MITOCHONDRION 0.000614904651595882 0.971598548759228 1486 Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene biological_process molecular_function mitochondrion YOR301W RAX1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.000608218082025326 0.971903074520195 1487 Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily cellular bud site selection molecular_function integral to membrane|cellular bud neck YMR270C RRN9 het FT NUCLEUS 0.000602422384208866 0.9721831460991 1488 Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YNL222W SSU72 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.000597760878518291 0.97238595552172 1489 Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo transcription initiation from RNA polymerase II promoter|mRNA 3'-end processing|mRNA cleavage|termination of RNA polymerase II transcription|termination of RNA polymerase II transcription, poly(A)-coupled|transcription antitermination|transcriptional start site selection at RNA polymerase II promoter|dephosphorylation of RNA polymerase II C-terminal domain|termination of RNA polymerase II transcription, exosome-dependent|snoRNA transcription|transcription elongation from RNA polymerase II promoter protein tyrosine phosphatase activity|phosphoprotein phosphatase activity|CTD phosphatase activity mRNA cleavage and polyadenylation specificity factor complex|nucleus YLR305C STT4 het FT LIPID METABOLISM|SIGNALING|MITOCHONDRION|PLASMA MEMBRANE 0.000597701548779959 0.97238869521279 1490 Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity mitochondrion|plasma membrane YIL108W YIL108W hom FT PROTEOLYSIS 0.000590953188797063 0.972700318822046 1491 Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cytoplasm YLR004C THI73 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.000588721649533559 0.972819585456892 1492 Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs transport transporter activity integral to membrane|endoplasmic reticulum YDR143C SAN1 hom FT PROTEOLYSIS|NUCLEUS 0.000585881371572148 0.972934526320438 1493 Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition nucleus-associated proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|response to misfolded protein|response to stress ubiquitin-protein ligase activity nucleus|cytoplasm YMR203W TOM40 het FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.000583405516041309 0.973072969565168 1494 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore protein import into mitochondrial matrix protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YBR165W UBS1 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.000582305572899488 0.973099651746337 1495 Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity protein export from nucleus|protein ubiquitination molecular_function nucleus YOR300W_d YOR300W hom 0.000580097046366221 0.973201639016348 1496 Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Unknown Unknown Unknown YER068W MOT2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.000570489892925502 0.973645291626002 1497 Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|protein polyubiquitination|protein ubiquitination ubiquitin-protein ligase activity|sequence-specific DNA binding CCR4-NOT core complex|cytoplasm YLR414C PUN1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.000562194578648503 0.974028370848594 1498 Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress fungal-type cell wall organization|protein oligomerization molecular_function integral to membrane|membrane raft|plasma membrane|cellular bud|cytoplasm YCL074W YCL074W hom FT NUCLEUS 0.000556982546964927 0.974269066551817 1499 Pseudogene: encodes fragment of Ty Pol protein transposition, RNA-mediated ribonuclease activity|peptidase activity|RNA binding|RNA-directed DNA polymerase activity|DNA-directed DNA polymerase activity retrotransposon nucleocapsid YCR084C TUP1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.000555034985465165 0.974359007068752 1500 General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes nucleosome positioning|regulation of transcription from RNA polymerase II promoter in response to osmotic stress|histone exchange|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by glucose|negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to hypoxia|negative regulation of transcription from RNA polymerase II promoter during mitosis|Unknown ubiquitin binding|histone deacetylase binding|histone binding|phosphatidylinositol-3,5-bisphosphate binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription|mediator complex binding nucleus|transcriptional repressor complex YNL080C EOS1 hom FT CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.000552145214526909 0.9744924604467 1501 Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene cellular response to oxidative stress|protein N-linked glycosylation molecular_function integral to membrane|endoplasmic reticulum membrane YAL068C PAU8 hom FT TRANSCRIPTION FROM RNA POL II 0.000541076118479251 0.975007378222915 1502 Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YHR049C-A_d YHR049C-A hom 0.000533562598818597 0.97535064668399 1503 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR039W CKB2 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.000525090549541488 0.975759999489673 1504 Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase inhibitor activity|protein kinase regulator activity protein kinase CK2 complex|UTP-C complex|CURI complex YGL067W NPY1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.000520391185910733 0.975958948955628 1505 NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member NADH metabolic process NAD+ diphosphatase activity peroxisome|cytoplasm YOR347C PYK2 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.000518192335963889 0.976060500967091 1506 Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication pyruvate metabolic process pyruvate kinase activity cytosol|mitochondrion YDL110C TMA17 hom FT NUCLEUS 0.000517867116128245 0.976100496204148 1507 Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion biological_process molecular_function ribosome|nucleus|cytoplasm YGL235W_p YGL235W hom 0.000514486842000417 0.976231636925572 1508 Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component YKL050C YKL050C hom 0.000511331087808602 0.976377384353581 1509 Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p biological_process molecular_function cellular_component YPR011C_p YPR011C hom FT MITOCHONDRION 0.000511171655904852 0.97638826790266 1510 Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies transport transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YDR127W ARO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.000499226377369431 0.977001854044659 1511 Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids aromatic amino acid family biosynthetic process shikimate kinase activity|shikimate 3-dehydrogenase (NADP+) activity|3-phosphoshikimate 1-carboxyvinyltransferase activity|3-dehydroquinate dehydratase activity|3-dehydroquinate synthase activity cytoplasm YDL184C RPL41A hom FT TRANSLATION|RIBOSOME 0.000491288335344566 0.977303070221801 1512 Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YGR177C ATF2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0004904083100662 0.977363987061351 1513 Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking steroid metabolic process|response to toxin|Unknown|sterol acetylation alcohol O-acetyltransferase activity integral to endoplasmic reticulum membrane|endoplasmic reticulum|cytoplasm YML022W APT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.000488991175743118 0.97740916781346 1514 Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication AMP biosynthetic process|adenine salvage adenine phosphoribosyltransferase activity nucleus|cytoplasm YPR088C SRP54 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS 0.000488501565123387 0.977431781229767 1515 Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|SRP-dependent cotranslational protein targeting to membrane|protein targeting to ER 7S RNA binding|GTP binding|signal sequence binding signal recognition particle, endoplasmic reticulum targeting YOR094W ARF3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.00048582478547644 0.977555412717252 1516 Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity intracellular protein transport|positive regulation of phosphatidylinositol biosynthetic process|endocytosis|actin filament bundle assembly|actin cortical patch localization|cellular bud site selection mRNA binding|GTPase activity cellular bud neck|cellular bud tip YGL181W GTS1 hom FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS|CYTOSKELETON 0.000474975761056588 0.978056498309971 1517 Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations endocytosis|ultradian rhythm|regulation of transcription from RNA polymerase II promoter|negative regulation of phosphatidylinositol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter ARF GTPase activator activity|repressing transcription factor binding|transcription factor binding transcription factor activity nucleus|cytoplasm|actin cortical patch YJL152W_d YJL152W hom 0.000474678816244531 0.978070213469179 1518 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR012C_d YBR012C hom 0.000470341564982071 0.978270541300699 1519 Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Unknown Unknown Unknown YGR041W BUD9 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.000469810809546807 0.978295055788815 1520 Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole cellular bud site selection molecular_function plasma membrane|cellular bud neck YJL001W PRE3 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.000467383584660263 0.978407164511748 1521 Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YPL234C VMA11 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.000465125842415147 0.978511445549004 1522 Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane|fungal-type vacuole YJR153W PGU1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0004640930038795 0.978559150618338 1523 Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins pseudohyphal growth|pectin catabolic process polygalacturonase activity extracellular region YLR028C ADE16 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.000453217266252891 0.979061487209631 1524 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine ascospore formation|'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process|aerobic respiration phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol YAR007C RFA1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.000448130506777439 0.979318055519682 1525 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|DNA replication|double-strand break repair via homologous recombination|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex|cytoplasm YJR078W BNA2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.000445791991276181 0.979404456066815 1526 Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp de novo NAD biosynthetic process from tryptophan tryptophan 2,3-dioxygenase activity|indoleamine 2,3-dioxygenase activity cytoplasm YKL010C UFD4 hom FT PROTEOLYSIS|MITOCHONDRION 0.000440101494054351 0.979682459387383 1527 Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress protein monoubiquitination|protein polyubiquitination ubiquitin-protein ligase activity mitochondrion|cytoplasm YDL039C PRM7 hom 0.00042739773896426 0.980254091456395 1528 Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements conjugation with cellular fusion molecular_function integral to membrane YFR014C CMK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.000414194113149324 0.980878252584334 1529 Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YFL014W HSP12 hom FT MEMBRANE ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|PLASMA MEMBRANE 0.00041233533162036 0.980949844761104 1530 Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension cellular response to oxidative stress|cell adhesion|plasma membrane organization|cellular response to osmotic stress|cellular response to heat lipid binding cytosol|endosome|nucleus|plasma membrane|cytoplasm YGR179C OKP1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.000409414766006239 0.981084751395347 1531 Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 attachment of spindle microtubules to kinetochore|chromosome segregation molecular_function COMA complex|condensed nuclear chromosome kinetochore YDR330W UBX5 hom FT PROTEOLYSIS|NUCLEUS 0.000401448441565625 0.981463796976435 1532 UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p proteasomal ubiquitin-dependent protein catabolic process molecular_function nucleus|cytoplasm YGR008C STF2 hom FT MITOCHONDRION 0.000393838338123761 0.981812405333698 1533 Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress cellular response to desiccation molecular_function cytoplasm YLR205C HMX1 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.000390521187400071 0.981965566346203 1534 ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants response to oxidative stress|cellular iron ion homeostasis|heme catabolic process heme oxygenase (decyclizing) activity endoplasmic reticulum|membrane|nuclear outer membrane YDR169C STB3 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.000382102083970326 0.982346403217381 1535 Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter by glucose sequence-specific DNA binding nucleus|cytoplasm YJR109C CPA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.000375650042607708 0.982644448240989 1536 Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor arginine biosynthetic process carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity carbamoyl-phosphate synthase complex YDL028C MPS1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.00037053071404468 0.982880931773187 1537 Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p protein phosphorylation|sister chromatid biorientation|spindle assembly|spindle pole body duplication associated with nuclear envelope|mitotic cell cycle spindle assembly checkpoint protein serine/threonine/tyrosine kinase activity|protein kinase activity condensed nuclear chromosome kinetochore|spindle pole body YKR060W UTP30 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS 0.000365837820403201 0.983097717786434 1538 Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit ribosomal small subunit biogenesis molecular_function nucleolus|nucleus|90S preribosome YOR298C-A MBF1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.000363905641023524 0.983189480870695 1539 Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity mitochondrion|nucleus YLR026C SED5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000359903774735721 0.983374319068119 1540 cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|cis-Golgi network YLR199C PBA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.000349660432895931 0.983845034052007 1541 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly proteasome assembly molecular_function cytoplasm YHR132W-A IGO2 hom FT NUCLEUS 0.000348520524081906 0.983897692812067 1542 Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p mRNA stabilization molecular_function nucleus|cytoplasm YBR169C SSE2 hom 0.000347825748520054 0.983929788413385 1543 Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication protein folding|protein refolding adenyl-nucleotide exchange factor activity cytoplasm YLR278C_p YLR278C hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.000347182070533499 0.983959523552741 1544 Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene biological_process sequence-specific DNA binding nucleus YKL166C TPK3 hom FT SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.000346761538257452 0.983998068828205 1545 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication protein phosphorylation|mitochondrion organization|Ras protein signal transduction cAMP-dependent protein kinase activity|protein kinase activity cAMP-dependent protein kinase complex|nucleus|cytoplasm YLR187W SKG3 hom FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0003364654350106 0.98445458901008 1546 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication biological_process molecular_function cellular bud|cellular bud neck|cytoplasm YML083C YML083C hom 0.000335935518407312 0.984479069182575 1547 Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component YOR373W NUD1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.000335366889189874 0.984505337749839 1548 Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance meiotic chromosome segregation|spindle pole body separation|exit from mitosis|establishment of spindle localization structural constituent of cytoskeleton spindle pole body YCR013C_d YCR013C het 0.000332648886531971 0.98463089965392 1549 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Unknown Unknown Unknown YDR110W FOB1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.000323428929170545 0.985056831193535 1550 Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases extrachromosomal circular DNA accumulation involved in cell aging|negative regulation of DNA replication|rDNA condensation|chromatin silencing at rDNA|replicative cell aging|protein localization to nucleolar rDNA repeats|DNA recombination rDNA spacer replication fork barrier binding nucleolus|rDNA heterochromatin YBR089W_d YBR089W het 0.000320169209508168 0.985207420486977 1551 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Unknown Unknown Unknown YGR292W MAL12 hom FT CARBOHYDRATE METABOLISM 0.000315753947552815 0.985422271718211 1552 Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YBR294W SUL1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.000313057908121317 0.985544571025421 1553 High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates sulfate transport|transmembrane transport sulfate transmembrane transporter activity integral to membrane|plasma membrane YOL034W SMC5 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.000297796277034011 0.986245100031748 1554 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair DNA repair|resolution of recombination intermediates|recombinational repair|rDNA separation damaged DNA binding|single-stranded DNA binding|ATPase activity Smc5-Smc6 complex|nucleus YGR136W LSB1 hom FT NUCLEUS 0.000296327292085124 0.986314986129579 1555 Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL125W MET13 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.000293732186485449 0.98642875082844 1556 Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway methionine biosynthetic process methylenetetrahydrofolate reductase (NADPH) activity mitochondrion YGR074W SMD1 het FT RNA PROCESSING|NUCLEUS 0.000292290326150304 0.986495362437037 1557 Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U4/U6 x U5 tri-snRNP complex|U2-type prespliceosome|U5 snRNP YLR280C_d YLR280C hom 0.00029082468402299 0.986563072822294 1558 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR169C PUS6 hom FT RNA PROCESSING|MITOCHONDRION 0.0002876838757554 0.986708173601285 1559 tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|cytoplasm YDR104C SPO71 hom FT CELL WALL ORG/BIOGENESIS 0.000283884604812147 0.986883694986159 1560 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains ascospore wall assembly molecular_function ascospore wall YJR108W ABM1 hom FT CYTOSKELETON ORGANIZATION 0.000281401475073725 0.986998412699587 1561 Protein of unknown function, required for normal microtubule organization microtubule cytoskeleton organization molecular_function cellular_component YGR278W CWC22 het FT RNA PROCESSING|NUCLEUS 0.00027389651708556 0.987345134676293 1562 Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex|U2-type catalytic step 1 spliceosome YBL099W ATP1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.000262563160976275 0.987870538283279 1563 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrial nucleoid|proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, catalytic core YNL166C BNI5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.000257588294661711 0.988119878568191 1564 Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner septin ring assembly|cytokinesis molecular_function cellular bud neck septin ring|cellular bud neck YNL157W IGO1 hom FT NUCLEUS 0.000255294335910516 0.988206306733163 1565 Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p mRNA stabilization molecular_function nucleus|cytoplasmic mRNA processing body|cytoplasm YER071C_p TDA2 hom FT SITE OF POLARIZED GROWTH 0.000242687296807826 0.988802051729466 1566 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function mating projection tip|cytoplasm YKL164C PIR1 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS 0.000242514871002775 0.988794962239334 1567 O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication intracellular protein transport|fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall YOR126C IAH1 hom FT KETONE METABOLISM|LIPID METABOLISM 0.000235519416135463 0.989126271290994 1568 Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing acetate metabolic process hydrolase activity, acting on ester bonds cellular_component YGR076C MRPL25 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.000225281208954563 0.989591171001221 1569 Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YML025C YML6 het FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0002249559796896 0.989607746674227 1570 Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YKL174C TPO5 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000223956582524183 0.989652370224656 1571 Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles polyamine transport polyamine transmembrane transporter activity integral to membrane|Golgi apparatus YDR358W GGA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.00022228819801675 0.989729451542492 1572 Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Golgi to vacuole transport|protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to endosome transport ubiquitin binding trans-Golgi network YDL176W YDL176W hom 0.000219788599039501 0.989852509032964 1573 Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene biological_process molecular_function cellular_component YGR054W YGR054W hom FT TRANSLATION 0.000216038806483108 0.99001818159491 1574 Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A translational initiation translation initiation factor activity cytosolic ribosome|cytosolic small ribosomal subunit|ribosome YMR139W RIM11 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION 0.000214653386990608 0.990082189963507 1575 Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress proteolysis|ascospore formation|protein phosphorylation|response to stress protein serine/threonine kinase activity|protein kinase activity cytoplasm YDL197C ASF2 hom FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.000213719870859076 0.990125319784363 1576 Anti-silencing protein that causes derepression of silent loci when overexpressed chromatin silencing at silent mating-type cassette molecular_function nuclear chromosome, telomeric region|nucleus YOL075C_p YOL075C hom FT NUCLEOTIDE METABOLISM 0.000212829324770802 0.990166464352787 1577 Putative ABC transporter transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YKL152C GPM1 het FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.000208813248779745 0.990352013425848 1578 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis gluconeogenesis|glycolysis phosphoglycerate mutase activity cytosol|mitochondrion YML053C_p YML053C hom FT NUCLEUS 0.000203672578994666 0.990590924396213 1579 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus|cytoplasm YJL119C_d YJL119C hom 0.000198696345128711 0.990819432691138 1580 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR346W REV1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.000198215813267474 0.990841634212747 1581 Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress error-prone translesion synthesis|error-free translesion synthesis deoxycytidyl transferase activity|DNA-directed DNA polymerase activity nuclear chromatin|mitochondrion|replication fork YJR037W_d YJR037W hom 0.000196542705680115 0.990929772890891 1582 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Unknown Unknown Unknown YOR079C ATX2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000196465777358643 0.990926550438066 1583 Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation cellular manganese ion homeostasis manganese ion transmembrane transporter activity integral to membrane|Golgi membrane YDL230W PTP1 hom FT MITOCHONDRION 0.000193461266119601 0.991061304066125 1584 Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation pseudohyphal growth|invasive growth in response to glucose limitation|protein dephosphorylation protein tyrosine phosphatase activity mitochondrion|cytoplasm YLR006C SSK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.000193448809692805 0.99106187957955 1585 Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate activation of MAPKKK activity involved in osmosensory signaling pathway|positive regulation of protein autophosphorylation|osmosensory signaling pathway via two-component system protein kinase activator activity|two-component response regulator activity|protein homodimerization activity|mitogen-activated protein kinase kinase kinase binding cytoplasm YNL042W BOP3 hom FT NUCLEUS 0.000190482419139512 0.991204183504691 1586 Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury cellular response to methylmercury molecular_function nucleus|cytoplasm YGR264C MES1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.000188700212456506 0.991281275212564 1587 Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs methionyl-tRNA aminoacylation methionine-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|cytoplasm YDL234C GYP7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM 0.000187345939496338 0.991343845722442 1588 GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking regulation of vacuole fusion, non-autophagic|vesicle-mediated transport Rab GTPase activator activity cytoplasm YGL139W FLC3 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.000184659922253549 0.991474308712136 1589 Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER FAD transport|transmembrane transport FAD transmembrane transporter activity mitochondrion|integral to membrane|endoplasmic reticulum YDR446W ECM11 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|NUCLEUS 0.000184458015251724 0.99148363034168 1590 Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure fungal-type cell wall organization|reciprocal meiotic recombination molecular_function nucleus YOR218C_d YOR218C het 0.000183111536510445 0.991539485171949 1591 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Unknown Unknown Unknown YER067W RGI1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|PLASMA MEMBRANE 0.000182454956830033 0.991569820754492 1592 Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress energy reserve metabolic process molecular_function cell periphery|nucleus|cytoplasm YDR215C_d YDR215C hom 0.000181708698310308 0.991609308169146 1593 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Unknown Unknown Unknown YPL107W_p YPL107W hom FT MITOCHONDRION 0.000173673496031676 0.99197554681374 1594 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene biological_process molecular_function mitochondrion YDR133C_d YDR133C hom 0.000173315404615615 0.992004039743711 1595 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Unknown Unknown Unknown YDR013W PSF1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.000168432415283614 0.99221769921017 1596 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YLL046C RNP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS 0.000166533393822934 0.99231117759939 1597 Ribonucleoprotein that contains two RNA recognition motifs (RRM) ribosome biogenesis RNA binding cytoplasm YFL021W GAT1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.000166064675094513 0.992327095578399 1598 Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p nitrogen catabolite activation of transcription from RNA polymerase II promoter|nitrogen catabolite activation of transcription sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity cytosol|nucleus YML079W_p YML079W hom FT NUCLEUS 0.000165016899640703 0.992422256429948 1599 Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm biological_process molecular_function nucleus|cytoplasm YLR267W_p BOP2 hom 0.000164079652650545 0.992418809403464 1600 Protein of unknown function biological_process molecular_function cellular_component YER044C ERG28 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.000154558076003529 0.992858735379389 1601 Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p ergosterol biosynthetic process protein binding, bridging endoplasmic reticulum membrane YDL036C PUS9 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.000153178707380569 0.992922466613587 1602 Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|nucleus|cytoplasm YER063W THO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA LOCALIZATION|NUCLEUS 0.000152646600676057 0.992952311379556 1603 Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation poly(A)+ mRNA export from nucleus|transcription, DNA-dependent|ribonucleoprotein complex assembly chromatin binding|double-stranded DNA binding|RNA binding nucleus YBL003C HTA2 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.000148389051568696 0.993143764351589 1604 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair|chromatin assembly or disassembly DNA binding replication fork protection complex|nuclear nucleosome YDR521W_d YDR521W hom 0.000148035945972333 0.993163139068509 1605 Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Unknown Unknown Unknown YIL092W_p YIL092W hom FT NUCLEUS 0.000144491201887319 0.993323858124266 1606 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YBL087C RPL23A hom FT TRANSLATION|RIBOSOME 0.000140977584604554 0.993515399999264 1607 Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YKL066W_d YKL066W hom 0.000137529819097191 0.993645498276102 1608 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 Unknown Unknown Unknown YNL188W KAR1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.000135682147404706 0.993737413612118 1609 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p karyogamy involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope protein binding half bridge of spindle pole body YKL117W SBA1 hom FT NUCLEUS 0.000130774117652111 0.993958537174237 1610 Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress protein folding|positive regulation of telomere maintenance via telomerase|regulation of telomerase activity chaperone binding nucleus|cytoplasm YDR235W PRP42 het FT RNA PROCESSING|NUCLEUS 0.000130010339905164 0.993993821277643 1611 U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats mRNA splicing, via spliceosome RNA binding U1 snRNP|U2-type prespliceosome YDR037W KRS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.00012977066331745 0.994003999535113 1612 Lysyl-tRNA synthetase lysyl-tRNA aminoacylation lysine-tRNA ligase activity cytoplasm YGR001C_p YGR001C hom 0.000128803725764147 0.994053113882495 1613 Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae biological_process methyltransferase activity cytoplasm YJL013C MAD3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS 0.000127766490014314 0.99409659984127 1614 Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover distributive segregation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex YDR031W MIC14 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.000125147863351992 0.994217590311464 1615 Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress aerobic respiration molecular_function nucleus|mitochondrial intermembrane space|cytoplasm YJR146W_p YJR146W hom 0.000123625003074218 0.994287952285632 1616 Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 biological_process molecular_function cellular_component YDR097C MSH6 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.000120974605249942 0.994410410916097 1617 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p mismatch repair|meiotic mismatch repair guanine/thymine mispair binding|four-way junction DNA binding|ATPase activity|single base insertion or deletion binding|ATP binding MutSalpha complex|nucleus YDL012C YDL012C hom FT PLASMA MEMBRANE 0.000118317465444432 0.994533181184285 1618 Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication biological_process molecular_function plasma membrane YML109W ZDS2 hom FT PROTEIN LOCALIZATION|CELL CYCLE|GENE SILENCING|NUCLEUS|SITE OF POLARIZED GROWTH 0.000115329085367685 0.994671256205048 1619 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|establishment of cell polarity|regulation of protein localization|chromatin silencing at rDNA|cell aging molecular_function nucleus|cellular bud neck|cellular bud tip|cytoplasm YMR034C_p YMR034C hom 0.000112764532010845 0.994789748876473 1620 Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process sterol transporter activity integral to membrane YIL147C SLN1 het FT SIGNALING|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.000111746208352375 0.994837569423181 1621 Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators negative regulation of protein kinase activity|protein autophosphorylation|peptidyl-histidine phosphorylation|osmosensory signaling pathway via two-component system osmosensor activity|protein histidine kinase activity|histidine phosphotransfer kinase activity plasma membrane|integral to plasma membrane YDR051C DET1 hom FT NUCLEUS 0.00010550729617446 0.995125062951039 1622 Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel intracellular sterol transport|dephosphorylation acid phosphatase activity nucleus|cytoplasm YPL152W-A_p YPL152W-A hom 0.000102847867328883 0.995247939694854 1623 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPR025C CCL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|NUCLEUS|MITOCHONDRION|RNA POL II, HOLOENZYME 9.96696049723678e-05 0.995394788865994 1624 Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|cyclin-dependent protein kinase regulator activity mitochondrion|holo TFIIH complex|TFIIK complex YLL052C AQY2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 9.72324253491392e-05 0.995507396984518 1625 Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains water transport water channel activity plasma membrane|endoplasmic reticulum membrane YKL130C SHE2 hom FT RNA LOCALIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 9.51047650988029e-05 0.995605704057902 1626 RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud mating type switching|intracellular mRNA localization mRNA binding cellular bud tip|cytoplasm YDR327W_d YDR327W het 9.37172109622923e-05 0.995669815075123 1627 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Unknown Unknown Unknown YEL052W AFG1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|RESPONSE TO OXIDATIVE STRESS|PEROXISOME ORGANIZATION|MITOCHONDRION 8.92855715910727e-05 0.995874576213767 1628 Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain protein import into peroxisome matrix|cellular response to oxidative stress|misfolded or incompletely synthesized protein catabolic process molecular_function mitochondrion|mitochondrial inner membrane YOR364W_d YOR364W hom 8.83870365408636e-05 0.995916092500014 1629 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Unknown Unknown Unknown YDR459C PFA5 hom FT PLASMA MEMBRANE 8.48227423217873e-05 0.996080778736659 1630 Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain protein palmitoylation palmitoyltransferase activity integral to membrane|plasma membrane YER130C_p YER130C hom FT NUCLEUS 8.21257278473927e-05 0.996207656636042 1631 Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress biological_process sequence-specific DNA binding cellular_component YAL003W EFB1 het FT TRANSLATION|RIBOSOME 7.44091127817215e-05 0.996562448285067 1632 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site translational elongation translation elongation factor activity|guanyl-nucleotide exchange factor activity ribosome|eukaryotic translation elongation factor 1 complex YER089C PTC2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 7.28089586186794e-05 0.996635870134979 1633 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication endoplasmic reticulum unfolded protein response|inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of cyclin-dependent protein kinase activity|protein dephosphorylation|DNA damage checkpoint protein serine/threonine phosphatase activity nucleus|cytoplasm YNL104C LEU4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 7.13769708836839e-05 0.996702034589179 1634 Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication leucine biosynthetic process 2-isopropylmalate synthase activity mitochondrion|cytoplasm YNL271C BNI1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 6.90044670915001e-05 0.996812606171486 1635 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 barbed-end actin filament capping|actin nucleation|actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assembly|establishment of mitotic spindle orientation|formin-nucleated actin cable assembly profilin binding incipient cellular bud site|actin filament|polarisome|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YGR025W_d YGR025W hom 6.7647739115755e-05 0.99687574083744 1636 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR497C ITR1 hom FT PLASMA MEMBRANE 6.70878982985753e-05 0.996900209707102 1637 Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress myo-inositol transport|transmembrane transport myo-inositol transmembrane transporter activity integral to membrane|plasma membrane|membrane YKL179C COY1 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 6.600690445947e-05 0.996950156684847 1638 Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Golgi vesicle transport molecular_function Golgi membrane YJR100C AIM25 hom FT MITOCHONDRION 6.47910827678711e-05 0.99700811927768 1639 Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YDR430C CYM1 hom FT PROTEOLYSIS|MITOCHONDRION 6.47273125623108e-05 0.997009279838347 1640 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis|proteolysis involved in cellular protein catabolic process metalloendopeptidase activity mitochondrion|mitochondrial intermembrane space YKL187C FAT3 hom FT MITOCHONDRION 5.93266488468275e-05 0.99726208761466 1641 Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|integral to membrane YDR447C RPS17B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 5.86720648877145e-05 0.997289060928245 1642 Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YKR032W_d YKR032W hom 5.86548547782708e-05 0.997292686122298 1643 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR138C TPO2 hom FT PLASMA MEMBRANE 5.83148685286519e-05 0.997307172512957 1644 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication spermine transport spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|plasma membrane YDR057W YOS9 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 5.69898996308579e-05 0.997366784982028 1645 ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family ER-associated protein catabolic process oligosaccharide binding luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum lumen YGL254W FZF1 hom FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 5.5569787442322e-05 0.997432400954316 1646 Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress positive regulation of sulfite transport by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YCR083W TRX3 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 5.54375473214935e-05 0.997438511080993 1647 Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p cellular response to oxidative stress disulfide oxidoreductase activity mitochondrion YKL024C URA6 het FT NUCLEOTIDE METABOLISM|NUCLEUS 5.51567181034973e-05 0.997451486735822 1648 Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) 'de novo' pyrimidine nucleobase biosynthetic process|nucleobase-containing compound metabolic process uridylate kinase activity|adenylate kinase activity nucleus|cytoplasm YDL046W NPC2 hom 5.37712842521115e-05 0.997517353278658 1649 Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes intracellular sterol transport molecular_function fungal-type vacuole lumen|fungal-type vacuole YPR037C ERV2 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 5.12399171854546e-05 0.997632461765404 1650 Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER protein thiol-disulfide exchange thiol oxidase activity integral to endoplasmic reticulum membrane YLR016C PML1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 4.99317360349693e-05 0.997693250057944 1651 Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p mRNA export from nucleus|mRNA splicing, via spliceosome|maintenance of RNA location molecular_function RES complex|nucleus|cytoplasm YHR121W LSM12 hom FT NUCLEUS 4.67656780847917e-05 0.997839193266933 1652 Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress RNA metabolic process RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YHR006W STP2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|PLASMA MEMBRANE 4.20430549410372e-05 0.998057401470435 1653 Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YGL152C_d YGL152C hom 3.83435754253759e-05 0.998228335554605 1654 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Unknown Unknown Unknown YGR077C PEX8 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 3.58936448872035e-05 0.9983415343974 1655 Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p protein targeting to peroxisome|peroxisome organization peroxisome targeting sequence binding peroxisomal matrix|peroxisomal membrane|extrinsic to intraperoxisomal membrane YLR288C MEC3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 3.14874364641541e-05 0.998545122984428 1656 DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 telomere maintenance via recombination|DNA damage checkpoint|DNA metabolic process|chromatin silencing at telomere DNA binding nucleus|checkpoint clamp complex YOR224C RPB8 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 3.04942399057614e-05 0.99859227460833 1657 RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YGR199W PMT6 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 2.90657702648774e-05 0.998657817145117 1658 Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases protein O-linked glycosylation|protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane YFL027C GYP8 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM 2.89062197568175e-05 0.998664387987199 1659 GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport vesicle-mediated transport Rab GTPase activator activity intracellular YIL059C_d YIL059C hom 2.78896186171913e-05 0.998711360005823 1660 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Unknown Unknown Unknown YNL161W CBK1 het FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH 2.7492003462862e-05 0.998729921209119 1661 Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress regulation of fungal-type cell wall organization|establishment or maintenance of cell polarity|protein phosphorylation|establishment or maintenance of actin cytoskeleton polarity|budding cell apical bud growth|Unknown protein serine/threonine kinase activity incipient cellular bud site|cellular bud|cellular bud neck|cellular bud tip|cytoplasm|cell cortex|nucleus|mating projection tip YAL007C ERP2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 2.65879225517283e-05 0.998771504831605 1662 Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles ER to Golgi vesicle-mediated transport molecular_function ER to Golgi transport vesicle YPL233W NSL1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 2.65515957662114e-05 0.998773183309689 1663 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YER156C_p YER156C hom FT NUCLEUS 2.16246545325029e-05 0.999000832535481 1664 Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 biological_process molecular_function nucleus|cytoplasm YPR056W TFB4 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 2.05435591220536e-05 0.999050784539836 1665 Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YLR042C_p YLR042C hom 1.9212367046386e-05 0.999112292263594 1666 Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene biological_process molecular_function fungal-type cell wall YKL097C_d YKL097C hom 1.77078198508782e-05 0.999181931865727 1667 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Unknown Unknown Unknown YJR106W ECM27 hom FT CELL WALL ORG/BIOGENESIS 1.74325527679943e-05 0.999194528582348 1668 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p fungal-type cell wall organization molecular_function integral to membrane YHL040C ARN1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 1.58680565146955e-05 0.999266816138841 1669 ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress siderophore transport siderophore transmembrane transporter activity endosome|integral to membrane|plasma membrane|cytoplasmic membrane-bounded vesicle YKR063C LAS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 1.35563111666567e-05 0.999373723751369 1670 Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability maturation of LSU-rRNA|maturation of 5.8S rRNA molecular_function mitochondrion|nucleus|preribosome|cytoplasm YKL201C MNN4 hom FT CARBOHYDRATE METABOLISM 8.73965492900663e-06 0.99959618403375 1671 Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication protein O-linked glycosylation|protein N-linked glycosylation|response to stress molecular_function membrane YLL017W YLL017W hom FT SIGNALING 6.9530285636628e-06 0.999678735142911 1672 Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Ras protein signal transduction Ras guanyl-nucleotide exchange factor activity membrane YHR178W STB5 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 6.52140142019075e-06 0.999699758654939 1673 Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro response to xenobiotic stimulus|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YLR401C DUS3 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 3.5422321932232e-06 0.999836331070458 1674 Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress tRNA modification tRNA dihydrouridine synthase activity nucleus|cytoplasm YOR296W_p YOR296W hom -3.30647145339301e-06 0.999847315542666 1675 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene biological_process molecular_function cytoplasm YPL012W RRP12 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME -3.66294377747494e-06 0.999830753588687 1676 Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosome biogenesis molecular_function ribosome|nucleus|90S preribosome YLR400W_d YLR400W hom -3.91617994494613e-06 0.999819052805259 1677 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL151C YKL151C hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM -4.3411279261764e-06 0.999799507812424 1678 NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress nicotinamide nucleotide metabolic process ATP-dependent NAD(P)H-hydrate dehydratase activity cytoplasm YDL014W NOP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -4.70592743465668e-06 0.99978256250308 1679 Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin box C/D snoRNA 3'-end processing|rRNA processing|snoRNA processing|rRNA methylation rRNA methyltransferase activity small-subunit processome|box C/D snoRNP complex|ribosome|nucleolus|90S preribosome YER030W CHZ1 hom FT CHROMATIN ORGANIZATION|NUCLEUS -9.27516400927914e-06 0.999571440826123 1680 Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin remodeling histone binding chromatin assembly complex|nucleus YGL243W TAD1 hom FT RNA PROCESSING|NUCLEUS -1.2038803114089e-05 0.999443746833792 1681 tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala tRNA modification tRNA-specific adenosine-37 deaminase activity nucleus YOR266W PNT1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -1.25852837046306e-05 0.999418496690297 1682 Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine protein insertion into mitochondrial membrane from inner side molecular_function mitochondrion|integral to mitochondrial inner membrane YBR210W ERV15 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION -1.33679474186702e-05 0.999382425802856 1683 Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle YHL015W RPS20 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME -1.45233922686925e-05 0.999328946361268 1684 Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YLR144C ACF2 hom FT CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION -1.53379511892578e-05 0.999291309659207 1685 Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress actin cytoskeleton organization glucan endo-1,3-beta-D-glucosidase activity intracellular YMR140W SIP5 hom FT RESPONSE TO NUTRIENT LEVELS -1.54025646464874e-05 0.999288324193915 1686 Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress cellular response to glucose starvation molecular_function cytoplasm YHL012W_p YHL012W hom -1.76197309109752e-05 0.999185880015832 1687 Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication biological_process molecular_function cellular_component YMR005W TAF4 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME -2.0133120235509e-05 0.999069748866125 1688 TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding transcription factor TFIID complex YPL076W GPI2 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM -2.22995903207139e-05 0.998970415538651 1689 Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane|endoplasmic reticulum YDR063W AIM7 hom FT CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON -2.68586132776246e-05 0.998758997574539 1690 Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss actin filament debranching|negative regulation of Arp2/3 complex-mediated actin nucleation molecular_function nucleus|actin cortical patch|cytoplasm YJL007C_d YJL007C hom -2.71090789313769e-05 0.998748172064479 1691 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL138W SRV2 hom FT SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -2.88165405097387e-05 0.99866853161336 1692 CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis cytoskeleton organization|Ras protein signal transduction cytoskeletal protein binding|adenylate cyclase binding mating projection tip|actin cortical patch YHR131C_p YHR131C hom -3.20206357072697e-05 0.998521810686767 1693 Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study biological_process molecular_function cytoplasm YOR375C GDH1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS -3.31585746157935e-05 0.99846790813787 1694 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication glutamate biosynthetic process|ammonia assimilation cycle glutamate dehydrogenase (NADP+) activity nucleus|cytoplasm YJR130C STR2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS -3.37776348558662e-05 0.998440934221438 1695 Cystathionine gamma-synthase, converts cysteine into cystathionine sulfur compound metabolic process|transsulfuration cystathionine gamma-synthase activity nucleus|cytoplasm YJR016C ILV3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION -3.67767555571486e-05 0.998300730336201 1696 Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids branched-chain amino acid biosynthetic process dihydroxy-acid dehydratase activity mitochondrion YCR089W FIG2 hom FT CELL WALL ORG/BIOGENESIS -3.81526316097832e-05 0.99823768384673 1697 Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating filamentous growth of a population of unicellular organisms|cell morphogenesis involved in conjugation with cellular fusion|invasive growth in response to glucose limitation|cytogamy molecular_function fungal-type cell wall|mating projection YBL035C POL12 het FT ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME -3.98301798521883e-05 0.998159647134272 1698 B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation telomere capping|lagging strand elongation|DNA replication initiation DNA-directed DNA polymerase activity nuclear envelope|alpha DNA polymerase:primase complex|nucleus YBR005W RCR1 hom FT VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -4.10515987567236e-05 0.998103211596699 1699 Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication fungal-type cell wall organization|vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane YEL066W HPA3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS -4.14577007855981e-05 0.998084447695408 1700 D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro D-amino acid metabolic process D-amino-acid N-acetyltransferase activity nucleus|cytoplasm YPL026C SKS1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN PHOSPHORYLATION|NUCLEUS -4.20477251778684e-05 0.99805718568266 1701 Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway glucose transport|protein phosphorylation|response to glucose stimulus|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YMR104C YPK2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS -4.59586764567109e-05 0.997878381184157 1702 Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication eisosome assembly|protein phosphorylation protein kinase activity nucleus|cytoplasm YNL180C RHO5 hom FT SIGNALING|NUCLEUS|PLASMA MEMBRANE -5.17750977094854e-05 0.997608804127496 1703 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Rho protein signal transduction GTPase activity endomembrane system|fungal-type vacuole membrane|plasma membrane|nucleus|cytoplasm YLR272C YCS4 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME -5.19866951978928e-05 0.99759795702196 1704 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation maintenance of rDNA|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation|chromatin silencing at silent mating-type cassette molecular_function nucleolus|nuclear condensin complex YMR096W SNZ1 hom FT COFACTOR METABOLISM -5.2751308629662e-05 0.997564445908724 1705 Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins pyridoxine metabolic process|thiamine biosynthetic process protein binding cellular_component YML074C FPR3 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS -5.86337858115943e-05 0.997291233564084 1706 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p meiotic recombination checkpoint peptidyl-prolyl cis-trans isomerase activity|macrolide binding nucleolus YER068C-A_d YER068C-A hom -6.15473535997442e-05 0.997157481232639 1707 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL127W PGM1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -6.25611717404006e-05 0.997109365751877 1708 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism glucose 6-phosphate metabolic process|galactose catabolic process|glucose 1-phosphate metabolic process|UDP-glucose metabolic process|trehalose biosynthetic process|glycogen biosynthetic process phosphoglucomutase activity cytoplasm YMR019W STB4 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -6.3675112374301e-05 0.997057896439564 1709 Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; biological_process sequence-specific DNA binding|DNA binding nucleus YML104C MDM1 hom FT MITOCHONDRION ORGANIZATION|CYTOSKELETON -6.49192501051065e-05 0.997001753413087 1710 Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) nuclear migration|mitochondrion inheritance|mitochondrion organization structural constituent of cytoskeleton|phosphatidylinositol-3-phosphate binding intermediate filament|integral to membrane|cytoplasm YLR226W BUR2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS -6.97086654013801e-05 0.996779118044485 1711 Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain|positive regulation of histone ubiquitination|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation|mitotic sister chromatid segregation cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YEL004W YEA4 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -7.0101638715784e-05 0.996762409969516 1712 Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER fungal-type cell wall chitin biosynthetic process|UDP-N-acetylglucosamine transport|UDP-glucose transport|transmembrane transport UDP-N-acetylglucosamine transmembrane transporter activity integral to membrane|endoplasmic reticulum YBR104W YMC2 hom FT MITOCHONDRION -7.31292484854272e-05 0.996621071265413 1713 Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication mitochondrial transport|transmembrane transport organic acid transmembrane transporter activity mitochondrion|integral to membrane YGR148C RPL24B hom FT TRANSLATION|RIBOSOME -7.3945726273748e-05 0.996583346229873 1714 Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YLR178C TFS1 hom FT SIGNALING|PROTEOLYSIS -7.46091650688141e-05 0.99655269230653 1715 Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress regulation of Ras protein signal transduction|regulation of proteolysis lipid binding|peptidase inhibitor activity|phospholipid binding fungal-type vacuole lumen|fungal-type vacuole membrane|cytoplasm YIR010W DSN1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE -7.77600999339376e-05 0.996407104695507 1716 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YKL202W_d YKL202W hom -8.11301766132245e-05 0.996251391862226 1717 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR252W BTT1 hom FT PROTEIN LOCALIZATION|NUCLEUS -8.248059498509e-05 0.99618899644605 1718 Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 'de novo' cotranslational protein folding unfolded protein binding nascent polypeptide-associated complex|CCR4-NOT complex YMR059W SEN15 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION -8.26354065747733e-05 0.99618184345423 1719 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity tRNA-intron endonuclease complex YGR011W_d YGR011W hom -8.34431213641711e-05 0.996144523402672 1720 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL123W RPS2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|NUCLEUS|RIBOSOME -8.51414864897392e-05 0.996066637915022 1721 Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 positive regulation of translational fidelity|rRNA export from nucleus structural constituent of ribosome|SSU rRNA binding small-subunit processome|cytosolic small ribosomal subunit YDL082W RPL13A hom FT TRANSLATION|RIBOSOME -8.68123272023181e-05 0.995988851050202 1722 Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJL137C GLG2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -8.77099604088636e-05 0.995951003433078 1723 Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication glycogen biosynthetic process glycogenin glucosyltransferase activity cytoplasm YDR219C MFB1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX -9.02593520136393e-05 0.995829583271727 1724 Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|mitochondrion organization molecular_function extrinsic to mitochondrial outer membrane|SCF ubiquitin ligase complex YHR066W SSF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -9.09529305220691e-05 0.995800043938883 1725 Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication conjugation with cellular fusion|ribosomal large subunit assembly|regulation of cell size rRNA binding nucleolus YJR022W LSM8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -9.56396520860162e-05 0.995580989967497 1726 Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|nucleus|U4/U6 x U5 tri-snRNP complex YPR108W RPN7 het FT PROTEOLYSIS|NUCLEUS -9.61445378757286e-05 0.995559649520519 1727 Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits ubiquitin-dependent protein catabolic process structural molecule activity nucleus|proteasome regulatory particle, lid subcomplex YOR155C ISN1 hom FT NUCLEOTIDE METABOLISM -9.69841425364485e-05 0.995518868717032 1728 Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms nicotinamide riboside biosynthetic process|inosine salvage|nicotinic acid riboside biosynthetic process IMP 5'-nucleotidase activity cellular_component YNR073C_p YNR073C hom -9.92385112791149e-05 0.995416758640754 1729 Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p biological_process mannitol dehydrogenase activity cellular_component YML113W DAT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000100219881657276 0.995369363743103 1730 DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability negative regulation of transcription from RNA polymerase II promoter AT DNA binding nucleus YNR046W TRM112 het FT RNA PROCESSING|NUCLEUS -0.000100226717145775 0.995369047914496 1731 Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress rRNA (guanine-N7)-methylation|peptidyl-glutamine methylation|tRNA methylation protein methyltransferase activity|zinc ion binding|tRNA (guanine-N2-)-methyltransferase activity|rRNA (guanine) methyltransferase activity eRF1 methyltransferase complex|nucleolus|nucleus|tRNA (m2G10) methyltransferase complex|cytoplasm YOL077W-A ATP19 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION -0.000102412107556233 0.995268073631061 1732 Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YNL013C_d YNL013C hom -0.000105016515964421 0.995148462549519 1733 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Unknown Unknown Unknown YFR042W KEG1 het FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000107229685826788 0.995045481401448 1734 Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability chromosome organization|(1->6)-beta-D-glucan biosynthetic process molecular_function integral to endoplasmic reticulum membrane|integral to membrane YDR099W BMH2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS -0.000108821417715323 0.99497193682654 1735 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|ascospore formation|Ras protein signal transduction|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|DNA damage checkpoint|DNA replication initiation|signal transduction involved in filamentous growth|pseudohyphal growth|fungal-type cell wall chitin biosynthetic process|pre-replicative complex assembly DNA replication origin binding|phosphoserine binding nucleus|plasma membrane YGR254W ENO1 hom FT MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000109443723798106 0.994945446090432 1736 Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|gluconeogenesis|glycolysis phosphopyruvate hydratase activity mitochondrion|phosphopyruvate hydratase complex|plasma membrane|fungal-type vacuole|cytoplasm YDL174C DLD1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000110032340031546 0.994915987252265 1737 D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane cellular carbohydrate metabolic process|aerobic respiration D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial inner membrane YOR261C RPN8 het FT PROTEOLYSIS -0.000110046452471027 0.994915335199864 1738 Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p ubiquitin-dependent protein catabolic process molecular_function proteasome storage granule|proteasome regulatory particle, lid subcomplex YDR365C ESF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000111304215939898 0.994861824061755 1739 Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels rRNA processing RNA binding nucleolus|nucleus YBR064W_d YBR064W hom -0.000117622501746891 0.994565291251049 1740 Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Unknown Unknown Unknown YJR067C YAE1 het FT NUCLEUS -0.000117784081323932 0.994557825635835 1741 Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species biological_process molecular_function nucleus|cytoplasm YOR252W TMA16 hom FT NUCLEUS -0.000118524497359903 0.994523615494071 1742 Protein of unknown function that associates with ribosomes biological_process molecular_function ribosome|nucleus YOL082W ATG19 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT -0.000118608112735909 0.994525474688714 1743 Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles vesicle organization|ER-associated protein catabolic process|protein processing|protein complex localization|CVT pathway protein binding, bridging pre-autophagosomal structure|cytoplasm|CVT complex|extrinsic to membrane YLR216C CPR6 hom -0.000122532247084677 0.994338441822687 1744 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding|protein refolding unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytoplasm YDR374C_p YDR374C hom -0.000123216360031853 0.994313627821879 1745 Putative protein of unknown function biological_process molecular_function cellular_component YKL215C OXP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000127750590751343 0.994097334447033 1746 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress glutathione metabolic process 5-oxoprolinase (ATP-hydrolyzing) activity cytoplasm YER011W TIR1 hom -0.000132167669815952 0.993893248971482 1747 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking response to stress structural constituent of cell wall fungal-type cell wall YBR276C PPS1 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS -0.000133217451556332 0.993844745210211 1748 Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle inactivation of MAPK activity|protein dephosphorylation|regulation of S phase of mitotic cell cycle protein tyrosine/serine/threonine phosphatase activity cellular_component YML081C-A ATP18 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION -0.000133316801644283 0.993840154873449 1749 Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YKL173W SNU114 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEOTIDE METABOLISM|NUCLEUS -0.00013576617887483 0.993726984768254 1750 GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 assembly of spliceosomal tri-snRNP|spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step GTP binding|GTPase activity|U5 snRNA binding U4/U6 x U5 tri-snRNP complex|U5 snRNP YHL042W_p YHL042W hom -0.000135847555325199 0.993723224883471 1751 Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component YJR147W HMS2 hom FT NUCLEUS -0.000136980337198882 0.993670886299898 1752 Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication pseudohyphal growth molecular_function nucleus|cytoplasm YAL059W ECM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|CELL WALL ORG/BIOGENESIS|NUCLEUS -0.000141242205182557 0.993473972966042 1753 Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm fungal-type cell wall organization|ribosomal large subunit export from nucleus molecular_function nucleolus|nucleus|preribosome, large subunit precursor YNL014W HEF3 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME -0.000142375520942327 0.993421609846745 1754 Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication translational elongation ATPase activity|translation elongation factor activity cytosolic ribosome YLR250W SSP120 hom -0.000146111340775459 0.993249002213581 1755 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm YGR194C XKS1 hom FT CARBOHYDRATE METABOLISM -0.000146335636976183 0.993238638973162 1756 Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains xylulose catabolic process xylulokinase activity cytoplasm YHR003C YHR003C hom FT MITOCHONDRION -0.000148312643282245 0.993147294668234 1757 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion YKL038W RGT1 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS -0.000149730541644568 0.993081783075879 1758 Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication glucose transport|regulation of glucose import|negative regulation of transcription from RNA polymerase II promoter|glucose metabolic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nucleus YDR471W RPL27B hom FT TRANSLATION|RIBOSOME -0.000149967114740625 0.993070852621183 1759 Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJL130C URA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|MITOCHONDRION -0.000151032976778348 0.993021606307511 1760 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP glutamine metabolic process|'de novo' pyrimidine nucleobase biosynthetic process|negative regulation of pyrimidine nucleobase metabolic process aspartate carbamoyltransferase activity|carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity mitochondrion|integral to membrane|cytoplasm YIL056W VHR1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000154622693004822 0.99285681511278 1761 Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter in response to biotin starvation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II regulatory region sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YDR400W URH1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS -0.000154657490960536 0.992854142092734 1762 Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways pyrimidine-containing compound salvage|NAD biosynthesis via nicotinamide riboside salvage pathway|pyrimidine nucleoside catabolic process|nicotinate nucleotide salvage ribosylpyrimidine nucleosidase activity|nicotinic acid riboside hydrolase activity|nicotinamide riboside hydrolase activity|uridine nucleosidase activity nucleus|cytoplasm YOR010C TIR2 hom -0.00015483289034104 0.992846038085042 1763 Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis response to stress molecular_function fungal-type cell wall YGL150C INO80 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME -0.000156231175766541 0.992781432889907 1764 ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription nucleosome positioning|histone exchange|transcription from RNA polymerase II promoter|DNA repair|nucleosome mobilization|chromatin remodeling|chromatin silencing at telomere|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter ATPase activity|ATP-dependent 3'-5' DNA helicase activity Ino80 complex YMR297W PRC1 hom FT PROTEOLYSIS|ENDOPLASMIC RETICULUM -0.00015700950579959 0.99274547160895 1765 Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family vacuolar protein catabolic process|phytochelatin biosynthetic process serine-type carboxypeptidase activity fungal-type vacuole lumen|endoplasmic reticulum|fungal-type vacuole|cytoplasm YPR137W RRP9 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000157679464088209 0.992717776843436 1766 Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein rRNA modification|rRNA processing snoRNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|90S preribosome YOR304W ISW2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS -0.000158472213617032 0.992677889974576 1767 ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth negative regulation of transcription from RNA polymerase II promoter by pheromones|nucleosome positioning|termination of RNA polymerase II transcription|negative regulation of antisense RNA transcription|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere single-stranded DNA binding|DNA translocase activity chromatin accessibility complex|nucleus YDL017W CDC7 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|PROTEIN PHOSPHORYLATION|GENE SILENCING|NUCLEUS -0.000159172040052026 0.992645555842927 1768 DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p negative regulation of exit from mitosis|regulation of chromatin silencing at telomere|protein phosphorylation|double-strand break repair via break-induced replication|DNA replication initiation protein serine/threonine kinase activity nucleus|Dbf4-dependent protein kinase complex YMR309C NIP1 het FT TRANSLATION -0.000163229035898805 0.992461484503732 1769 eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YIR013C GAT4 hom FT NUCLEUS -0.000163759844222412 0.992444874949029 1770 Protein containing GATA family zinc finger motifs transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YMR308C PSE1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RNA LOCALIZATION|NUCLEUS -0.000165454765204051 0.992355275183054 1771 Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p mRNA export from nucleus|regulation of protein desumoylation|protein import into nucleus|regulation of mitosis protein transporter activity|nuclear localization sequence binding integral to membrane|nucleus|cytoplasm YDL086W_p YDL086W hom FT MITOCHONDRION -0.000167296843260047 0.992274777108336 1772 Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene biological_process carboxymethylenebutenolidase activity mitochondrion|cytoplasm YNL092W_p YNL092W hom -0.000171517813001298 0.99207514523145 1773 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YLL012W YEH1 hom FT LIPID METABOLISM -0.000172561295355209 0.992026933493765 1774 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes sterol metabolic process sterol esterase activity integral to membrane|lipid particle YEL060C PRB1 hom FT PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS -0.000174566400842596 0.991934292220019 1775 Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress vacuolar protein catabolic process|sporulation resulting in formation of a cellular spore|cellular response to starvation serine-type endopeptidase activity fungal-type vacuole lumen|fungal-type vacuole YLR367W RPS22B hom FT TRANSLATION|RIBOSOME -0.000175275846097625 0.991901513964499 1776 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YDL008W APC11 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -0.000175483944150602 0.9918918992842 1777 Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YBR280C SAF1 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX -0.000176600976071652 0.991842722946328 1778 F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity SCF ubiquitin ligase complex YAL043C PTA1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS -0.000177982844961482 0.99177644355148 1779 Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII tRNA processing|termination of RNA polymerase II transcription, exosome-dependent|mRNA cleavage|termination of RNA polymerase II transcription, poly(A)-coupled|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YOL045W PSK2 hom FT SIGNALING|TRANSLATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000179334151846887 0.991716480849836 1780 PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication regulation of (1->6)-beta-D-glucan biosynthetic process|negative regulation of glycogen biosynthetic process|protein phosphorylation protein serine/threonine kinase activity mitochondrion|cytoplasm YDR305C HNT2 hom FT NUCLEUS|MITOCHONDRION -0.000180866500921854 0.991643211341774 1781 Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins nucleoside catabolic process hydrolase activity|nucleoside-triphosphatase activity mitochondrion|nucleus|cytoplasm YNL020C ARK1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000181666947636029 0.991607480258455 1782 Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|cytokinesis|actin cortical patch assembly protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YML052W SUR7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE -0.000181792467368141 0.991600429395698 1783 Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants endocytosis|ascospore formation molecular_function cell cortex|eisosome|mitochondrion|membrane raft|integral to membrane|plasma membrane|actin cortical patch YGR172C YIP1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS -0.000182945256824255 0.991552210213511 1784 Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 vesicle-mediated transport|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus molecular_function integral to membrane|ER to Golgi transport vesicle|integral to Golgi membrane|endoplasmic reticulum membrane YPR136C_d YPR136C het -0.000184229967732175 0.991487810949977 1785 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Unknown Unknown Unknown YMR012W CLU1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION -0.000186684421277031 0.991374409397362 1786 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation|mitochondrion organization molecular_function eukaryotic translation initiation factor 3 complex|cytoplasm YMR229C RRP5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000196418698060985 0.990926017705893 1787 RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding small-subunit processome|nucleolus|90S preribosome YDR429C TIF35 het FF|FT TRANSLATION -0.00019735416181341 0.990881444230445 1788 eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity eukaryotic translation initiation factor 3 complex YDR055W PST1 hom FT CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE -0.000197935259176835 0.990868241563264 1789 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 fungal-type cell wall organization molecular_function fungal-type cell wall|plasma membrane YDL123W SNA4 hom -0.000201930135104563 0.990670025389352 1790 Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated biological_process molecular_function fungal-type vacuole lumen|fungal-type vacuole membrane YPL027W SMA1 hom FT MEMBRANE ORGANIZATION -0.000203856486481426 0.990581024414608 1791 Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p ascospore-type prospore membrane assembly molecular_function prospore membrane YKR082W NUP133 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE -0.000207301318660662 0.990421867128234 1792 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 poly(A)+ mRNA export from nucleus|double-strand break repair|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|tRNA export from nucleus|telomere tethering at nuclear periphery|nuclear pore distribution|mRNA export from nucleus in response to heat stress|positive regulation of transcription, DNA-dependent|negative regulation of transcription from RNA polymerase II promoter|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore outer ring YDL131W LYS21 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS|MITOCHONDRION -0.000207948525224538 0.990391965092581 1793 Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication lysine biosynthetic process via aminoadipic acid homocitrate synthase activity nucleus YDL054C MCH1 hom -0.000207956422095189 0.990393032874229 1794 Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport transport monocarboxylic acid transmembrane transporter activity|transporter activity integral to membrane|membrane YJL029C VPS53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS -0.000208273269866196 0.990376961347089 1795 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Golgi to vacuole transport|retrograde transport, endosome to Golgi molecular_function Golgi apparatus|GARP complex|cytoplasm YOR054C VHS3 hom FT CELL CYCLE|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|COFACTOR METABOLISM -0.000209607484504709 0.990315318435432 1796 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis coenzyme A biosynthetic process|cellular monovalent inorganic cation homeostasis phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex YDL146W LDB17 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000211191926253367 0.990242114694643 1797 Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck endocytosis molecular_function cellular bud|cellular bud neck|cytoplasm|actin cortical patch YJL143W TIM17 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000212270356653926 0.990192289533687 1798 Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex protein import into mitochondrial matrix|mitochondrial genome maintenance protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane presequence translocase complex YOL080C REX4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000217659864773121 0.989943286457291 1799 Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly ribosomal large subunit assembly|rRNA processing 3'-5' exonuclease activity nucleolus|nucleus YJR072C NPA3 het FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION -0.000217760754183221 0.989938625229768 1800 Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress cellular protein complex localization|nucleocytoplasmic transport|mitotic sister chromatid cohesion ATPase activity|GTPase activity cytoplasm YDR380W ARO10 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000219283519807726 0.98986827143527 1801 Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway L-phenylalanine catabolic process|methionine catabolic process to 3-methylthiopropanol|tryptophan catabolic process|branched-chain amino acid catabolic process to alcohol via Ehrlich pathway|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway|leucine catabolic process pyruvate decarboxylase activity|carboxy-lyase activity|phenylpyruvate decarboxylase activity cytoplasm YDR530C APA2 hom FT NUCLEOTIDE METABOLISM|NUCLEUS -0.000220011117836911 0.989834655467643 1802 Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication nucleoside catabolic process bis(5'-nucleosyl)-tetraphosphatase activity nucleus|cytoplasm YML007C-A_p YML007C-A hom FF|FT MITOCHONDRION -0.000222168329795939 0.989734989598351 1803 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria biological_process molecular_function mitochondrion YLR459W GAB1 het FT CELL CYCLE|LIPID METABOLISM|CELL DIVISION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000223047958854747 0.98969434969021 1804 GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|GPI-anchor transamidase complex YBR115C LYS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS -0.000223239742857637 0.989685489044846 1805 Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p lysine biosynthetic process via aminoadipic acid L-aminoadipate-semialdehyde dehydrogenase activity cytoplasm YOL125W TRM13 hom FT RNA PROCESSING|NUCLEUS -0.000230700860057632 0.989340777696759 1806 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases tRNA methylation tRNA methyltransferase activity nucleus|cytoplasm YOR275C RIM20 hom FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS|NUCLEUS -0.000233930495260907 0.989191565815868 1807 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation proteolysis|response to pH|protein processing|invasive growth in response to glucose limitation|sporulation resulting in formation of a cellular spore molecular_function nucleus|cytoplasm YIL089W YIL089W hom FT ENDOPLASMIC RETICULUM -0.000234482575436705 0.989167674621552 1808 Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking biological_process molecular_function fungal-type vacuole lumen|integral to membrane|endoplasmic reticulum YER014C-A BUD25 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION -0.000234810348820444 0.98915091590173 1809 Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cellular bud site selection molecular_function cellular_component YLR385C SWC7 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME -0.000241762576050192 0.988829718631663 1810 Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin chromatin remodeling molecular_function Swr1 complex|nucleus YMR177W MMT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000242391034793542 0.98880068353091 1811 Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p cellular iron ion homeostasis molecular_function mitochondrion|integral to membrane YKL171W NNK1 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION -0.000243010312204614 0.988772072626692 1812 Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm proteolysis|protein phosphorylation protein kinase activity cytoplasm YPL109C_p YPL109C hom FT MITOCHONDRION -0.000244051763273558 0.988723957135827 1813 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDR153C ENT5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM -0.000248512764130645 0.98851785742837 1814 Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin late endosome to vacuole transport via multivesicular body sorting pathway|early endosome to Golgi transport|Golgi to endosome transport clathrin binding|phosphatidylinositol-3,5-bisphosphate binding endosome|clathrin vesicle coat|cytoplasm YEL016C NPP2 hom FT NUCLEOTIDE METABOLISM -0.000248857116489853 0.988507092070565 1815 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication nucleoside triphosphate metabolic process nucleotide diphosphatase activity|nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|phosphodiesterase I activity cellular_component YOL048C RRT8 hom -0.000251116378444844 0.988397569943414 1816 Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|lipid particle YLR326W_p YLR326W hom -0.000252891940443334 0.988315538786644 1817 Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cellular_component YDR434W GPI17 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.00025449003580439 0.988241706713307 1818 Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|endoplasmic reticulum|GPI-anchor transamidase complex YGL202W ARO8 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000254952712260305 0.988220331060217 1819 Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis aromatic amino acid family metabolic process aromatic-amino-acid:2-oxoglutarate aminotransferase activity|2-aminoadipate transaminase activity cytoplasm YBR153W RIB7 het FT OXIDATION-REDUCTION PROCESS -0.000255429846771325 0.988198287453807 1820 Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway riboflavin biosynthetic process 5-amino-6-(5-phosphoribosylamino)uracil reductase activity cellular_component YER147C SCC4 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME -0.000257135219725385 0.988119499325654 1821 Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX double-strand break repair|establishment of protein localization to chromatin|rDNA condensation|mitotic chromosome condensation|mitotic sister chromatid cohesion molecular_function nuclear chromatin|SMC loading complex YCL017C NFS1 het FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|COFACTOR METABOLISM|NUCLEUS|MITOCHONDRION -0.000258743631311272 0.988045190913 1822 Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria cellular iron ion homeostasis|iron-sulfur cluster assembly|mitochondrial tRNA thio-modification|tRNA thio-modification|tRNA wobble uridine modification cysteine desulfurase activity mitochondrion|nucleus YLR314C CDC3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000259099641519309 0.988035884454568 1823 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells septin ring disassembly|maintenance of cell polarity|cell cycle cytokinesis|septin ring assembly structural molecule activity|GTP binding|1-phosphatidylinositol binding mating projection base|septin filament array|cellular bud neck septin ring|septin complex|prospore membrane|ascospore wall YLR213C CRR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM -0.000259394189509119 0.988015135235617 1824 Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation ascospore wall assembly hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ascospore wall YLR283W_p YLR283W hom FT MITOCHONDRION -0.000260452164819288 0.987975230692686 1825 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene biological_process molecular_function mitochondrion YDL219W DTD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION -0.000261185287301665 0.987932386912156 1826 D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Unknown D-leucyl-tRNA(Leu) deacylase activity|D-tyrosyl-tRNA(Tyr) deacylase activity cytoplasm YKL131W_d YKL131W hom -0.000261610852229918 0.987912725925261 1827 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML084W_d YML084W hom -0.00026548797367151 0.987733604281651 1828 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL044W_p YBL044W hom -0.000265589623322323 0.987738058777021 1829 Putative protein of unknown function; YBL044W is not an essential protein biological_process molecular_function cellular_component YKL091C YKL091C hom FT NUCLEUS -0.000266540422197588 0.98768498163709 1830 Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study biological_process phosphatidylcholine binding|phosphatidylinositol transporter activity|phosphatidylinositol binding|phosphatidylcholine transporter activity nucleus YBR078W ECM33 hom FF|FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE -0.000268971209994626 0.987572680518412 1831 GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p fungal-type cell wall organization molecular_function mitochondrion|fungal-type cell wall|plasma membrane YDR411C DFM1 hom FT SIGNALING|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX -0.000270104746372626 0.987522172500499 1832 Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p endoplasmic reticulum unfolded protein response|ER-associated protein catabolic process molecular_function Doa10p ubiquitin ligase complex|integral to membrane|endoplasmic reticulum|Hrd1p ubiquitin ligase ERAD-L complex YCR005C CIT2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.000270277938989263 0.987519759597459 1833 Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication glyoxylate cycle|glutamate biosynthetic process|citrate metabolic process citrate (Si)-synthase activity mitochondrion|peroxisome YLR222C UTP13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000270957049271247 0.987480935970089 1834 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YML100W-A_p YML100W-A hom -0.000274168425461663 0.98733257272381 1835 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR009W GAL3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000275734552267633 0.987267818484844 1836 Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication maintenance of protein location|galactose metabolic process|positive regulation of transcription from RNA polymerase II promoter by galactose protein binding nucleus|cytoplasm YJL200C ACO2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.00027970949867665 0.987084289248326 1837 Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process aconitate hydratase activity mitochondrion YPL230W USV1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000279773147396131 0.987073639695414 1838 Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to salt stress sequence-specific DNA binding nucleus YDR295C HDA2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME -0.000279831521740176 0.987070942861211 1839 Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance negative regulation of transcription from RNA polymerase II promoter|gene silencing by RNA|histone deacetylation|gene silencing involved in chronological cell aging histone deacetylase activity|chromatin binding|DNA binding nucleus|HDA1 complex YPR126C_d YPR126C hom -0.00027986268977201 0.987102316191368 1840 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR059C REX2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000281840856488016 0.986978113768306 1841 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease U5 snRNA 3'-end processing|U4 snRNA 3'-end processing|mitochondrial genome maintenance|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 3'-5'-exoribonuclease activity|3'-5' exonuclease activity mitochondrion YEL019C MMS21 het FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME -0.000286707209664656 0.986753294245576 1842 SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination DNA repair SUMO ligase activity nuclear envelope|Smc5-Smc6 complex|nucleus|cytoplasm YBR129C OPY1 hom FT MITOCHONDRION -0.000289784724686633 0.986611117383817 1843 Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YOR161C-C_p YOR161C-C hom -0.000291953162315423 0.986510938872877 1844 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YML009C MRPL39 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME -0.000294832139606194 0.986377934842092 1845 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOL086W-A MHF1 hom FT RESPONSE TO DNA DAMAGE -0.000295426251932087 0.986350487873659 1846 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 response to DNA damage stimulus molecular_function cellular_component YDL135C RDI1 hom FT MEMBRANE ORGANIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH -0.000296832023007735 0.986293724555116 1847 Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|actin filament organization|small GTPase mediated signal transduction Rho GDP-dissociation inhibitor activity cytosol|plasma membrane|cellular bud neck|cellular bud tip YDR179C CSN9 hom FT SIGNALING|NUCLEUS -0.000296988761743736 0.98628648782619 1848 Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YMR114C YMR114C hom FT NUCLEUS -0.000297414467470728 0.986293506426143 1849 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene biological_process molecular_function ribosome|nucleus|cytoplasm YHR019C DED81 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION -0.000298700638574878 0.986205390899939 1850 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity cytosol YPL133C RDS2 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000299605644906074 0.98615740755903 1851 Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|cellular response to xenobiotic stimulus|positive regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YNL301C RPL18B hom FT TRANSLATION|RIBOSOME -0.000299941112498408 0.986141909604552 1852 Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL114W_p YDL114W hom FT OXIDATION-REDUCTION PROCESS -0.000301424818004503 0.986081672706312 1853 Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene biological_process molecular_function integral to membrane YDL063C SYO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.000304686498348674 0.985931079742647 1854 Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus ribosomal large subunit biogenesis molecular_function nucleus|cytoplasm YMR291W TDA1 hom FT PROTEIN PHOSPHORYLATION|NUCLEUS -0.000306291801956388 0.985848521016939 1855 Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress protein phosphorylation protein kinase activity nucleus|cytoplasm YNL045W LAP2 hom FT KETONE METABOLISM|LIPID METABOLISM|PROTEOLYSIS|NUCLEUS -0.000313654577260694 0.985508378574435 1856 Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus cellular lipid metabolic process|protein catabolic process aminopeptidase activity|leukotriene-A4 hydrolase activity|epoxide hydrolase activity nucleus|cytoplasm YPR105C COG4 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS -0.000316631082300459 0.985370871971914 1857 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|intra-Golgi vesicle-mediated transport|retrograde transport, vesicle recycling within Golgi|macroautophagy|CVT pathway molecular_function Golgi transport complex YCR057C PWP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|NUCLEUS -0.000316970670982178 0.985366104315029 1858 Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|establishment of cell polarity|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytokinesis snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|cytoplasm|90S preribosome YGR017W_p YGR017W hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS -0.000319337304635106 0.985254652963298 1859 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus|cytoplasm YJL035C TAD2 het FT RNA PROCESSING|NUCLEUS -0.000320435971011877 0.985195096883945 1860 Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YOR159C SME1 het FT RNA PROCESSING|NUCLEUS -0.000320783082170959 0.985179061366591 1861 Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YGR213C RTA1 hom FT PLASMA MEMBRANE -0.000322700911762584 0.985090463373169 1862 Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein biological_process molecular_function integral to membrane|plasma membrane YLL011W SOF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000324777651597194 0.984994524408919 1863 Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YMR210W YMR210W hom FT KETONE METABOLISM|LIPID METABOLISM -0.000326449175565691 0.984917305323249 1864 Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification medium-chain fatty acid biosynthetic process transferase activity, transferring acyl groups cellular_component YPR167C MET16 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS -0.000327836814675198 0.984853200921516 1865 3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process phosphoadenylyl-sulfate reductase (thioredoxin) activity cytoplasm YDR082W STN1 het FT NUCLEUS|CHROMOSOME -0.00032852736163545 0.984828089979997 1866 Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping telomere capping|negative regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YOR100C CRC1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION -0.000329398204518137 0.984781069919403 1867 Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation fatty acid metabolic process carnitine:acyl carnitine antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YPL190C NAB3 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS -0.000329534739710499 0.984774762454887 1868 RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing nuclear mRNA surveillance|mRNA 3'-end processing|antisense RNA transcript catabolic process|CUT catabolic process|termination of RNA polymerase II transcription, exosome-dependent|tRNA 3'-end processing|snRNA 3'-end processing|snoRNA 3'-end processing RNA binding|transcription regulatory region RNA binding Nrd1 complex|nucleus YLR393W ATP10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000329704374142288 0.984766925918681 1869 Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 mitochondrial proton-transporting ATP synthase complex assembly unfolded protein binding integral to mitochondrial membrane|mitochondrion|mitochondrial inner membrane YDL193W NUS1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM -0.000333759642433765 0.984600290395298 1870 Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY protein glycosylation prenyltransferase activity nuclear envelope|lipid particle|endoplasmic reticulum YGR112W SHY1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000335316321911218 0.984507673771947 1871 Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane YMR195W ICY1 hom -0.000335825118500606 0.984484169248731 1872 Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation biological_process molecular_function fungal-type vacuole membrane YLR113W HOG1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS -0.000336587276181501 0.984448960404057 1873 Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter by pheromones|protein phosphorylation|hyperosmotic response|response to arsenic-containing substance|osmosensory signaling pathway|positive regulation of transcription from RNA polymerase II promoter|cellular response to heat MAP kinase activity nucleus|cytoplasm YPL246C RBD2 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS -0.000337750744000696 0.984395212584886 1874 Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p biological_process molecular_function COPI-coated vesicle|integral to membrane|Golgi apparatus YJL131C AIM23 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000338169703594216 0.984375858251276 1875 Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss positive regulation of mitochondrial translational initiation ribosome binding|translation initiation factor activity mitochondrion YBR079C RPG1 het FT TRANSLATION -0.000340570639451579 0.984264944359187 1876 eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation translation reinitiation|translational initiation translation initiation factor activity incipient cellular bud site|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YDR512C EMI1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MITOCHONDRION ORGANIZATION -0.000341689095846214 0.984213276130488 1877 Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs ascospore formation|mitochondrial respiratory chain complex assembly|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YJL077W-B_p YJL077W-B hom -0.000341855591599139 0.984205584695796 1878 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR350C ATP22 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000345895060932801 0.984026126754254 1879 Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane proton-transporting ATP synthase complex biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrial inner membrane YOR122C PFY1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE|CYTOSKELETON -0.000347230035939826 0.98395730775763 1880 Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress positive regulation of formin-nucleated actin cable assembly|sequestering of actin monomers|intracellular transport actin monomer binding|phosphatidylinositol-4,5-bisphosphate binding|proline-rich region binding cytosol|extrinsic to plasma membrane YOR230W WTM1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING|NUCLEUS -0.000349445079719753 0.983857389453681 1881 Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats negative regulation of chromatin silencing at silent mating-type cassette|maintenance of protein location in nucleus|negative regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of chromatin silencing at telomere|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter protein binding|transcription corepressor activity nucleus YOR271C_p FSF1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000349869492077886 0.983842607413223 1882 Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis biological_process molecular_function mitochondrion|integral to membrane YJR151W-A_p YJR151W-A hom -0.000353156679760181 0.983693255866462 1883 Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis biological_process molecular_function cellular_component YDL094C_d YDL094C hom -0.000353406501907429 0.983671982681066 1884 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Unknown Unknown Unknown YDR109C_p YDR109C hom FT CARBOHYDRATE METABOLISM -0.000356470632553325 0.983542715065406 1885 Putative kinase biological_process molecular_function cellular_component YJL198W PHO90 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS -0.000358013853220834 0.98345914503429 1886 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication phosphate ion transport phosphate ion transmembrane transporter activity integral to membrane|membrane YBR054W YRO2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000361070895438464 0.983317924918981 1887 Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication biological_process molecular_function mitochondrion|integral to membrane|cellular bud YOR028C CIN5 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000361694465758626 0.983289119141846 1888 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication hyperosmotic salinity response|response to drug|hyperosmotic response|regulation of transcription from RNA polymerase II promoter repressing transcription factor binding|sequence-specific DNA binding nucleus|cytoplasm YNL303W_d YNL303W hom -0.000362378141898716 0.983277515939111 1889 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR002W MPP10 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000364367594364893 0.983165634443773 1890 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|Mpp10 complex|nucleolus|nucleus|90S preribosome YHR164C DNA2 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.000364836824009308 0.983143958505748 1891 Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress DNA repair|lagging strand elongation|meiotic DNA double-strand break processing|DNA strand renaturation|replicative cell aging|telomere maintenance|DNA-dependent DNA replication ATP-dependent DNA helicase activity|single-stranded DNA specific endodeoxyribonuclease activity|5'-flap endonuclease activity nuclear chromosome, telomeric region|nucleus|cytoplasm YKL206C ADD66 hom FT PROTEIN COMPLEX ORG/BIOGENESIS -0.00036514656411504 0.983129650151154 1892 Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly proteasome assembly molecular_function cytosol|cytoplasm YPL189W GUP2 hom -0.000366006988233698 0.983089903136344 1893 Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog glycerol transport O-acyltransferase activity integral to membrane|membrane YEL044W IES6 hom FT NUCLEUS -0.000366762431635212 0.983062585655221 1894 Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes biological_process molecular_function nucleus YLR049C_p YLR049C hom -0.000367466849697567 0.983022465402635 1895 Putative protein of unknown function biological_process molecular_function cellular_component YOR003W YSP3 hom FT PROTEOLYSIS -0.000368045837794751 0.982995719305765 1896 Putative precursor to the subtilisin-like protease III protein catabolic process peptidase activity cellular_component YEL026W SNU13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000368531771802274 0.982975810270084 1897 RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mRNA splicing, via spliceosome RNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|U4/U6 x U5 tri-snRNP complex YJL043W_p YJL043W hom FT MITOCHONDRION -0.000369174965405105 0.982943559814479 1898 Putative protein of unknown function; YJL043W is a non-essential gene biological_process molecular_function mitochondrion|cytoplasm YOL047C_p YOL047C hom -0.000369253268160131 0.982939942660153 1899 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function integral to membrane|cytoplasm YOR119C RIO1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS -0.000370274422246258 0.982892771019727 1900 Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA regulation of exit from mitosis|S phase of mitotic cell cycle|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) protein kinase activity nucleus|cytoplasm YDR029W_d YDR029W hom -0.000370376656551541 0.982951941868188 1901 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR376W-A_p YOR376W-A hom -0.000370821947670154 0.982867478414391 1902 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YKL196C YKT6 het FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE -0.000371625221780344 0.98283037168455 1903 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus intra-Golgi vesicle-mediated transport|vacuole fusion, non-autophagic|vesicle fusion palmitoyltransferase activity|SNAP receptor activity SNARE complex|endosome|mitochondrion|Golgi apparatus|fungal-type vacuole|membrane YDR355C_d YDR355C het -0.000371723179118345 0.982846340731608 1904 Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Unknown Unknown Unknown YOR083W WHI5 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS -0.000372007508966663 0.982812712187383 1905 Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 negative regulation of transcription involved in G1/S phase of mitotic cell cycle|regulation of cell size RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription SBF transcription complex|nucleus|cytoplasm YMR244C-A COA6 hom FT NUCLEUS|MITOCHONDRION -0.000374080849088478 0.98278146800705 1906 Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YDL129W YDL129W hom FT NUCLEUS -0.000377150013849448 0.982575158485593 1907 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YGR117C_p YGR117C hom -0.000377162439621312 0.982574584489341 1908 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YDR428C BNA7 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM -0.000380744520013482 0.982416982983959 1909 Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine NAD biosynthetic process arylformamidase activity cellular_component YJL173C RFA3 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.000382295938917555 0.982337448337132 1910 Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YDL163W_d YDL163W het -0.000382809021455178 0.982313747159184 1911 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Unknown Unknown Unknown YJR099W YUH1 hom FT PROTEOLYSIS -0.000382868711467976 0.982310989857986 1912 Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p protein deubiquitination ubiquitin-specific protease activity cytoplasm YCR022C_d YCR022C hom -0.000384480125122016 0.982236552815362 1913 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Unknown Unknown Unknown YLR164W SHH4 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.000384532965419496 0.982234111932939 1914 Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner biological_process molecular_function mitochondrion|mitochondrial inner membrane YLR394W CST9 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.00038486813307663 0.982218629343377 1915 SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate synaptonemal complex assembly|protein sumoylation|synapsis|reciprocal meiotic recombination SUMO ligase activity condensed nuclear chromosome|nuclear chromosome YIL006W YIA6 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000385325824473116 0.982205450371648 1916 Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs transport|mitochondrial pyruvate transport|NAD transmembrane transport pyruvate secondary active transmembrane transporter activity|transporter activity|NAD transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YGR085C RPL11B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME -0.000386530750079906 0.98216047217909 1917 Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YDR046C BAP3 hom FT MITOCHONDRION|PLASMA MEMBRANE -0.000387307768310882 0.982105934092269 1918 Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YGL250W RMR1 hom FT CELL CYCLE|NUCLEUS -0.000388326860786524 0.982058858765885 1919 Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus meiotic gene conversion|reciprocal meiotic recombination molecular_function nucleus|cytoplasm YLL035W GRC3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME -0.000389442596186672 0.982007319247742 1920 Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated termination of RNA polymerase I transcription|rRNA processing polynucleotide 5'-hydroxyl-kinase activity nucleolar chromatin YER053C PIC2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000389940109132118 0.981984337499123 1921 Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature phosphate ion transport|transmembrane transport inorganic phosphate transmembrane transporter activity mitochondrion|integral to membrane YGR042W YGR042W hom FT NUCLEUS -0.000390033285734289 0.982004226169916 1922 Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YLR083C EMP70 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT -0.000392933225712455 0.981846075980077 1923 Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments cellular copper ion homeostasis|vacuolar transport|pseudohyphal growth|endosomal transport|invasive growth in response to glucose limitation molecular_function endosome|fungal-type vacuole membrane|integral to membrane YLR127C APC2 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -0.000393232403762004 0.981840382764543 1924 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p mitotic metaphase/anaphase transition|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|exit from mitosis|cyclin catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YKR102W FLO10 hom -0.000393369309369826 0.981825931942444 1925 Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth filamentous growth of a population of unicellular organisms|flocculation via cell wall protein-carbohydrate interaction|flocculation|invasive growth in response to glucose limitation mannose binding fungal-type cell wall YMR137C PSO2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS -0.00039430294816463 0.981782804350504 1926 Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress DNA repair|double-strand break repair damaged DNA binding|5'-3' exonuclease activity nucleus YDL057W_p YDL057W hom -0.000395177013133851 0.981742428701484 1927 Putative protein of unknown function; YDL057W is not an essential gene biological_process molecular_function cellular_component YNL310C ZIM17 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000397217760123804 0.981648160737566 1928 Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix protein folding|protein stabilization|protein import into mitochondrial matrix|response to unfolded protein|mitochondrion organization chaperone binding mitochondrion|mitochondrial matrix YDR279W RNH202 hom FT NUCLEUS -0.00039753701355911 0.981633413527719 1929 Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome RNA catabolic process|DNA replication, removal of RNA primer ribonuclease H activity ribonuclease H2 complex|nucleus YKL124W SSH4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT -0.000397663132463727 0.981627587744205 1930 Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|vesicle-mediated transport molecular_function fungal-type vacuole YDL043C PRP11 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -0.000400160233100608 0.981512239912072 1931 Subunit of the SF3a splicing factor complex, required for spliceosome assembly spliceosomal complex assembly RNA binding U2 snRNP|U2-type prespliceosome YPL223C GRE1 hom -0.000400384747763751 0.981501868991449 1932 Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway biological_process molecular_function cytoplasm YCR016W_p YCR016W hom FT NUCLEUS -0.000400856263202095 0.981480088467936 1933 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleolus|nucleus YKL094W YJU3 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION -0.000401054802068334 0.981470917445852 1934 Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family triglyceride metabolic process serine hydrolase activity|acylglycerol lipase activity mitochondrial outer membrane|lipid particle|plasma membrane|membrane|cytoplasm YGR295C COS6 hom -0.000401075883280742 0.98148376028887 1935 Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function integral to membrane|fungal-type vacuole YKL014C URB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000402697687464307 0.981395028427937 1936 Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YLR344W RPL26A hom FT TRANSLATION|RIBOSOME -0.000403357528481972 0.9813645487553 1937 Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDL210W UGA4 hom -0.000405609391164394 0.981260529907931 1938 Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane gamma-aminobutyric acid transport|putrescine transport|transmembrane transport putrescine transmembrane transporter activity|gamma-aminobutyric acid:hydrogen symporter activity fungal-type vacuole membrane|integral to membrane YJL172W CPS1 hom FT PROTEOLYSIS -0.00040786355015238 0.981167644738199 1939 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions nitrogen compound metabolic process|proteolysis involved in cellular protein catabolic process carboxypeptidase activity fungal-type vacuole lumen YLR206W ENT2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000409963944787393 0.981059383712628 1940 Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus actin filament organization|endocytosis|actin cortical patch assembly clathrin binding mating projection tip|actin cortical patch YOL057W YOL057W hom FT PROTEOLYSIS|NUCLEUS -0.000412118239402384 0.980962711173142 1941 Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers proteolysis dipeptidyl-peptidase activity nucleus|cytoplasm YPL005W AEP3 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000413262147928009 0.980921269623577 1942 Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA mRNA metabolic process|mitochondrial translational initiation molecular_function mitochondrion|mitochondrial inner membrane|cytoplasm|extrinsic to membrane YOL046C_d YOL046C hom -0.000414534895584819 0.980848242961623 1943 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Unknown Unknown Unknown YPL119C-A_p YPL119C-A hom -0.000414759192884115 0.980837882285017 1944 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YKL089W MIF2 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE -0.000415583092413092 0.980799824981696 1945 Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 chromosome segregation|mitotic spindle organization in nucleus centromeric DNA binding condensed nuclear chromosome, centromeric region|condensed nuclear chromosome inner kinetochore YMR112C MED11 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME -0.000415786472345768 0.980790430527664 1946 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter TFIIH-class transcription factor binding|TFIIH-class transcription factor recruiting transcription factor activity core mediator complex YLR352W_p YLR352W hom -0.00041712355760656 0.98074016288368 1947 Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene biological_process molecular_function SCF ubiquitin ligase complex YBR272C HSM3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|NUCLEUS -0.000417263452758664 0.980750965119919 1948 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation mismatch repair|proteasome regulatory particle assembly molecular_function cytosol|proteasome regulatory particle, base subcomplex|nucleus|cytoplasm YJR110W YMR1 hom FT LIPID METABOLISM -0.000417613301603732 0.980706046395218 1949 Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family phosphatidylinositol dephosphorylation mRNA binding|phosphatidylinositol-3-phosphatase activity cytoplasm YIL146C ATG32 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION -0.000417672886320854 0.980703294086534 1950 Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane fungal-type cell wall organization|mitochondrion degradation molecular_function integral to mitochondrial outer membrane YDR499W LCD1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.000417806302008222 0.980697131413903 1951 Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress establishment of protein localization|telomere maintenance via telomerase|DNA damage checkpoint damaged DNA binding nuclear chromosome YGR277C CAB4 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS -0.000418211339059557 0.980681302605634 1952 Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved coenzyme A biosynthetic process pantetheine-phosphate adenylyltransferase activity nucleus|cytoplasm YIL045W PIG2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -0.000421020967207023 0.980548641509401 1953 Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase regulation of glycogen biosynthetic process protein phosphatase type 1 regulator activity cytoplasm YLR319C BUD6 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000421499171446981 0.980526552640763 1954 Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate bipolar cellular bud site selection|positive regulation of formin-nucleated actin cable assembly|cellular bud site selection|Rho protein signal transduction|extrachromosomal circular DNA localization involved in cell aging|establishment of cell polarity|response to osmotic stress|cytokinesis cytoskeletal regulatory protein binding polarisome|actin cap|spindle pole body|mating projection tip YJL205C NCE101 hom FT PROTEIN LOCALIZATION -0.000421844672805781 0.980516404870505 1955 Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences protein secretion molecular_function cellular_component YFR028C CDC14 het FT CELL CYCLE|NUCLEUS|CYTOSKELETON -0.000422745462555876 0.980503953684041 1956 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis regulation of exit from mitosis|mitotic cell cycle|protein dephosphorylation phosphoprotein phosphatase activity nucleolus|RENT complex|nucleus|spindle pole body YDR299W BFR2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000424554476845016 0.980385424509434 1957 Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock rRNA processing molecular_function nucleolus|90S preribosome YJL041W NSP1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE -0.000425499851307662 0.98034175668093 1958 FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|ncRNA export from nucleus|ribosomal large subunit export from nucleus|tRNA export from nucleus|protein import into nucleus|ribosomal small subunit export from nucleus nucleocytoplasmic transporter activity|phospholipid binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore nuclear basket|nuclear pore YKL184W SPE1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM -0.000429865611113323 0.980163796289319 1959 Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation pantothenate biosynthetic process|putrescine biosynthetic process ornithine decarboxylase activity cytoplasm YLR353W BUD8 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE -0.000430266814149687 0.980121566614914 1960 Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole pseudohyphal growth|cellular bud site selection molecular_function incipient cellular bud site|plasma membrane|cellular bud tip YGL016W KAP122 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE -0.000431678606760426 0.980056355003881 1961 Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance protein import into nucleus|regulation of cell size protein transporter activity integral to membrane|nuclear pore|cytoplasm YBL019W APN2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS -0.000434169174508163 0.979941314398731 1962 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII base-excision repair DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity nucleus YJL109C UTP10 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|CHROMOSOME -0.000435967048534088 0.979870283328548 1963 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding mitochondrion|90S preribosome|small-subunit processome|preribosome, small subunit precursor|integral to membrane|t-UTP complex|rDNA heterochromatin|nucleolus YGL248W PDE1 hom FT SIGNALING|NUCLEOTIDE METABOLISM -0.000436635961986689 0.979827372627462 1964 Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation cAMP-mediated signaling 3',5'-cyclic-AMP phosphodiesterase activity cellular_component YNL042W-B_p YNL042W-B hom -0.000439633211631503 0.979688929178109 1965 Putative protein of unknown function biological_process molecular_function cellular_component YKL088W CAB3 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM -0.000439969685430712 0.97967338743661 1966 Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|purine nucleotide binding phosphopantothenoylcysteine decarboxylase complex|cytoplasm YJR068W RFC2 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME -0.000440927628120023 0.979638251878645 1967 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|cell cycle checkpoint|leading strand elongation DNA clamp loader activity|purine nucleotide binding Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|Ctf18 RFC-like complex YNL209W SSB2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RESPONSE TO NUTRIENT LEVELS -0.00044110540570039 0.979633083414102 1968 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication cellular response to glucose starvation|cytoplasmic translation|ribosomal subunit export from nucleus|rRNA processing|regulation of translational fidelity|'de novo' cotranslational protein folding unfolded protein binding|ATPase activity plasma membrane|polysome|cytoplasm YER014W HEM14 hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION -0.000441368557259571 0.9796087735601 1969 Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides heme biosynthetic process oxygen-dependent protoporphyrinogen oxidase activity mitochondrion|mitochondrial inner membrane YER025W GCD11 het FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME -0.000441846163954156 0.979586712942007 1970 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met positive regulation of translational fidelity GTP binding|tRNA binding|translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YBL021C HAP3 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS -0.000444000742483424 0.979487193312449 1971 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex YBL020W RFT1 het FT MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000444810264775644 0.979449801692684 1972 Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein glycolipid translocation molecular_function|glycolipid-translocating activity integral to membrane|endoplasmic reticulum membrane YDR454C GUK1 het FT NUCLEOTIDE METABOLISM|NUCLEUS -0.000446082681274134 0.979391029203673 1973 Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins GMP metabolic process guanylate kinase activity nucleus|cytoplasm YBR241C_p YBR241C hom -0.000446960896870465 0.979350464786613 1974 Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene transport|transmembrane transport substrate-specific transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|membrane YIL121W QDR2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.000447505666637891 0.979325302122013 1975 Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper drug transmembrane transport|potassium ion import drug transmembrane transporter activity integral to membrane|integral to plasma membrane YDR042C_p YDR042C hom -0.000448961991917444 0.979270406472935 1976 Putative protein of unknown function; expression is increased in ssu72-ts69 mutant biological_process molecular_function cellular_component YOR320C GNT1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS -0.000449547255936972 0.979231002235909 1977 N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus protein N-linked glycosylation acetylglucosaminyltransferase activity Golgi medial cisterna YGR019W UGA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000451376295556948 0.979149628883896 1978 Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress nitrogen utilization|gamma-aminobutyric acid catabolic process 4-aminobutyrate transaminase activity intracellular YBR050C REG2 hom FT TRANSCRIPTION FROM RNA POL II -0.000454167774991171 0.979017584077076 1979 Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease negative regulation of transcription from RNA polymerase II promoter protein phosphatase type 1 regulator activity protein phosphatase type 1 complex YJL106W IME2 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|NUCLEUS -0.000457084641882502 0.978892302527356 1980 Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p protein phosphorylation|regulation of meiosis protein kinase activity nucleus YKL162C_p YKL162C hom FT MITOCHONDRION -0.000458889270380435 0.978799503451249 1981 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion YGL004C RPN14 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS -0.000462236902571277 0.978667175356305 1982 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|cytoplasm YDR505C PSP1 hom FT MITOCHONDRION -0.000462901086843701 0.978639721107851 1983 Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication biological_process molecular_function mitochondrion|cytoplasm YLL038C ENT4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON -0.000465797980875878 0.978480400648801 1984 Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome endocytosis|actin filament organization|establishment of protein localization to plasma membrane|actin cortical patch assembly clathrin binding actin cortical patch YHL045W_d YHL045W hom -0.000466232168892165 0.978460346280866 1985 Putative protein of unknown function; not an essential gene Unknown Unknown Unknown YDR422C SIP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION -0.000468000108264204 0.978378688463843 1986 Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions signal transduction|protein phosphorylation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|fungal-type vacuole YGL229C SAP4 hom FT CELL CYCLE|MITOCHONDRION -0.000468950254702069 0.978334803068293 1987 Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity mitochondrion|cytoplasm YBL107C MIC23 hom -0.00047225714445867 0.978182064845166 1988 Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) biological_process molecular_function cytoplasm YOR034C AKR2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS -0.000472588727141569 0.978166749788424 1989 Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication endocytosis|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus YGR258C RAD2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|NUCLEUS -0.000473637618517368 0.978118303912957 1990 Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein nucleotide-excision repair, DNA incision, 3'-to lesion|transcription from RNA polymerase II promoter single-stranded DNA specific endodeoxyribonuclease activity nucleotide-excision repair factor 3 complex YCL034W LSB5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON -0.000474175090708228 0.978103277725566 1991 Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat endocytosis|actin filament organization|actin cortical patch localization|actin cytoskeleton organization molecular_function cell cortex|cytoplasm|actin cortical patch YFR018C_p YFR018C hom FT PROTEOLYSIS -0.000474378510980922 0.978084083846067 1992 Putative protein of unknown function biological_process molecular_function cellular_component YEL071W DLD3 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS -0.000474382357748469 0.978083906173097 1993 D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm lactate metabolic process|filamentous growth D-lactate dehydrogenase (cytochrome) activity cytoplasm YAL034W-A MTW1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE -0.000475503931815231 0.978032103402261 1994 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly chromosome segregation|protein localization to kinetochore molecular_function nuclear MIS12/MIND type complex|spindle pole YER024W YAT2 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM -0.000477107066307799 0.977958058690631 1995 Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane carnitine metabolic process|alcohol metabolic process carnitine O-acetyltransferase activity cytosol|cytoplasm YKL062W MSN4 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS -0.000479597701287906 0.977843022961773 1996 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|cellular response to acidity|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|replicative cell aging|age-dependent response to oxidative stress involved in chronological cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YLR398C SKI2 hom FT TRANSLATION -0.000481168346320938 0.977770479330071 1997 Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay RNA helicase activity Ski complex YLR203C MSS51 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000483506194222263 0.977662501118113 1998 Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis mitochondrial respiratory chain complex IV biogenesis|positive regulation of mitochondrial translation translation regulator activity extrinsic to mitochondrial inner membrane|mitochondrion YKR050W TRK2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.000487035068877362 0.977499513713219 1999 Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication cellular potassium ion homeostasis potassium ion transmembrane transporter activity integral to membrane|plasma membrane YDR437W GPI19 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM -0.000490062397859934 0.977359691842741 2000 Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|nuclear envelope|integral to endoplasmic reticulum membrane|endoplasmic reticulum YLR349W_d YLR349W hom -0.000491887239999236 0.977275409022247 2001 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Unknown Unknown Unknown YBR084W MIS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION -0.000494222550041828 0.977167549916189 2002 Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase nucleobase-containing compound metabolic process|folic acid biosynthetic process formate-tetrahydrofolate ligase activity|methylenetetrahydrofolate dehydrogenase (NADP+) activity|methenyltetrahydrofolate cyclohydrolase activity mitochondrion YGL141W HUL5 hom FT PROTEOLYSIS|NUCLEUS -0.000495774690289406 0.977095862680774 2003 Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD ER-associated protein catabolic process|protein polyubiquitination ubiquitin-ubiquitin ligase activity proteasome complex|nucleus|cytoplasm YFR054C_d YFR054C hom -0.000498364208966246 0.976976263439661 2004 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR023C AHC1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE -0.000499126136951419 0.976941073211127 2005 Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex YKL012W PRP40 het FT RNA PROCESSING|NUCLEUS -0.000499725315835727 0.976913399706181 2006 U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex mRNA splicing, via spliceosome RNA binding U1 snRNP|nucleus|U2-type prespliceosome YJL135W_d YJL135W hom -0.000502624963468694 0.976779477801209 2007 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Unknown Unknown Unknown YGR191W HIP1 het FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.00050621466692961 0.976613686174983 2008 High-affinity histidine permease, also involved in the transport of manganese ions manganese ion transport|histidine transport high affinity L-histidine transmembrane transporter activity integral to membrane|plasma membrane YAL037C-A_p YAL037C-A hom -0.000506902831252611 0.976581903196203 2009 Putative protein of unknown function biological_process molecular_function cellular_component YMR150C IMP1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000507043727491798 0.976575395885554 2010 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein processing involved in protein targeting to mitochondrion endopeptidase activity mitochondrion|mitochondrial inner membrane peptidase complex YOR019W YOR019W hom FT CELL CYCLE -0.000509426782003685 0.976465334447564 2011 Protein of unknown function that may interact with ribosomes, based on co-purification experiments mitochondrion degradation molecular_function ribosome YDR130C FIN1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE -0.000509577317154738 0.976458381992501 2012 Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress intermediate filament cytoskeleton organization|mitotic cell cycle spindle checkpoint|mitotic spindle stabilization|mitotic sister chromatid segregation microtubule binding|structural constituent of cytoskeleton spindle microtubule|condensed nuclear chromosome kinetochore|intermediate filament|nucleus|spindle pole body|astral microtubule|spindle YLR154C RNH203 hom FT NUCLEUS -0.000513901250910812 0.976272841365346 2013 Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome RNA catabolic process|DNA replication, removal of RNA primer ribonuclease H activity ribonuclease H2 complex|nucleus|cytoplasm YGR026W_p YGR026W hom FT PLASMA MEMBRANE -0.000515183534187797 0.976199460541899 2014 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function integral to membrane YDR402C DIT2 hom FT CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS -0.000515445884455492 0.976187344034732 2015 N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s ascospore wall assembly oxidoreductase activity cellular_component YJR058C APS2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE -0.000516409803862963 0.976142825978265 2016 Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex vesicle-mediated transport molecular_function AP-2 adaptor complex YGR082W TOM20 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION|MITOCHONDRION -0.000517270531535436 0.976103073834757 2017 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins tRNA import into mitochondrion|protein import into mitochondrial matrix|mitochondrial outer membrane translocase complex assembly protein channel activity|mitochondrion targeting sequence binding mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YNL335W_p DDI3 hom -0.000519152372039582 0.976019737601715 2018 Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants biological_process molecular_function cellular_component YJR009C TDH2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000520646558284088 0.975947154825346 2019 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication gluconeogenesis|reactive oxygen species metabolic process|glycolysis|apoptotic process glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YIR044C YIR044C hom -0.000520829796604574 0.975938692140957 2020 Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family biological_process molecular_function cellular_component YDR089W YDR089W hom FT MEMBRANE ORGANIZATION -0.000521644475905138 0.975911845438861 2021 Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress cellular membrane organization molecular_function integral to membrane|membrane YER038W-A_d YER038W-A hom -0.000522400779438444 0.975887730874173 2022 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Unknown Unknown Unknown YMR288W HSH155 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -0.000522466341310081 0.975877504978146 2023 U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 spliceosomal complex assembly|mRNA splicing, via spliceosome mRNA binding U2 snRNP|U2-type prespliceosome YDL133C-A RPL41B hom FT TRANSLATION|RIBOSOME -0.000523701954327904 0.975806044672208 2024 Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YNL254C RTC4 hom FT NUCLEUS -0.000523907847258485 0.975796535757641 2025 Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YER157W COG3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS -0.000524103343577669 0.975787507001108 2026 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|ER to Golgi vesicle-mediated transport|macroautophagy|retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway protein transporter activity Golgi transport complex YOR249C APC5 het FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -0.000527218759826939 0.975643625760918 2027 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YJL083W TAX4 hom FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS -0.000527376267008083 0.975636351528093 2028 EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication autophagy|fungal-type cell wall organization|inositol lipid-mediated signaling|CVT pathway|cellular response to starvation molecular_function pre-autophagosomal structure YJL204C RCY1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH -0.000532824371117278 0.975384740265397 2029 F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth early endosome to Golgi transport|endocytosis SNARE binding endosome|Golgi apparatus|site of polarized growth YLR334C_d YLR334C hom -0.000535698549706403 0.975252002284694 2030 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Unknown Unknown Unknown YHR068W DYS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000540478580869076 0.975075946926625 2031 Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric peptidyl-lysine modification to hypusine transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm YFL034C-B MOB2 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH -0.000546145889572724 0.97476951934557 2032 Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p positive regulation of protein phosphorylation|establishment or maintenance of cell polarity|Unknown|budding cell apical bud growth protein kinase activator activity nucleus|cellular bud|cellular bud neck|mating projection tip|cellular bud tip YFL045C SEC53 het FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM -0.000548080559813612 0.974721719360482 2033 Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen protein targeting to ER phosphomannomutase activity cytosol YGR012W YGR012W hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION -0.000548249180528162 0.974672385489846 2034 Putative cysteine synthase, localized to the mitochondrial outer membrane cysteine biosynthetic process cysteine synthase activity mitochondrial outer membrane|mitochondrion YAR008W SEN34 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION -0.000548805919935344 0.974646674299932 2035 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YGR149W_p YGR149W hom -0.00054910792055031 0.974632727399581 2036 Putative protein of unknown function; predicted to be an integal membrane protein biological_process molecular_function integral to membrane YNL227C JJJ1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|RIBOSOME -0.000557506024460687 0.974244891845104 2037 Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|endocytosis|regulation of cell size|rRNA processing ATPase activator activity cytosol|mitochondrion|cytosolic large ribosomal subunit|nucleolus|cytoplasm YIL099W SGA1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -0.000557883448405917 0.974227462049982 2038 Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation ascospore formation|glycogen catabolic process glucan 1,4-alpha-glucosidase activity fungal-type vacuole YFL008W SMC1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME -0.000558191037093343 0.974263270240985 2039 Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure double-strand break repair|mitotic sister chromatid cohesion|mitotic sister chromatid segregation double-stranded DNA binding|ATPase activity|AT DNA binding|DNA secondary structure binding nuclear mitotic cohesin complex YFR017C IGD1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -0.000558493282779836 0.974226231857981 2040 Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication negative regulation of glycogen catabolic process enzyme inhibitor activity cytoplasm YHR166C CDC23 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|CHROMOSOME|UBIQUITIN LIGASE COMPLEX -0.00055867275856199 0.974191010999458 2041 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity|cyclin binding anaphase-promoting complex YEL037C RAD23 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|MITOCHONDRION -0.00055958949152809 0.974152528835162 2042 Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover proteasomal ubiquitin-dependent protein catabolic process|nucleotide-excision repair, DNA damage recognition|ER-associated protein catabolic process|protein deglycosylation damaged DNA binding|ubiquitin binding|peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity|protein binding, bridging proteasome complex|mitochondrion|nucleotide-excision repair factor 2 complex YLR011W LOT6 hom FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS -0.000561828202475281 0.974045290310085 2043 FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions cellular response to oxidative stress|cytoplasmic sequestering of transcription factor NAD(P)H dehydrogenase (quinone) activity|transcription factor binding cytosol|nucleus|cytoplasm YMR176W ECM5 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS|CHROMOSOME -0.000562929810871128 0.973994417438762 2044 Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress fungal-type cell wall organization molecular_function nucleus|Snt2C complex YFL047W RGD2 hom FT SIGNALING|SITE OF POLARIZED GROWTH -0.000564231111415928 0.973934322812289 2045 GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress small GTPase mediated signal transduction Rho GTPase activator activity cellular bud neck|cytoplasm YKL022C CDC16 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -0.000565402579310864 0.973880224040682 2046 Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|negative regulation of DNA-dependent DNA replication initiation|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YDR119W_p VBA4 hom -0.000565748564480197 0.973895428129678 2047 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function fungal-type vacuole membrane|integral to membrane YNL129W NRK1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM -0.000565788928628353 0.973874071959556 2048 Nicotinamide riboside kinase, catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis nicotinamide riboside metabolic process|NAD biosynthesis via nicotinamide riboside salvage pathway|NAD biosynthetic process nicotinic acid riboside kinase activity|ribosylnicotinamide kinase activity cytoplasm YLR128W DCN1 hom -0.00056839169933018 0.973742186042634 2049 Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation protein neddylation|positive regulation of ubiquitin-protein ligase activity ubiquitin binding|NEDD8 ligase activity|small conjugating protein binding cellular_component YDR088C SLU7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -0.000569031889727142 0.973724378619181 2050 RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity spliceosomal complex YFR024C-A LSB3 hom FT MITOCHONDRION|SITE OF POLARIZED GROWTH -0.000569237121394256 0.973703144470961 2051 Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication actin cortical patch localization molecular_function mitochondrion|cellular bud neck|cytoplasm YKL108W SLD2 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS -0.000570027059815236 0.973666665189576 2052 Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress DNA replication preinitiation complex assembly|DNA strand renaturation|DNA replication initiation|double-strand break repair via break-induced replication|mitotic cell cycle DNA replication checkpoint DNA replication origin binding|single-stranded DNA binding DNA replication preinitiation complex YDR504C SPG3 hom -0.000570631414329936 0.973638756192559 2053 Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component YGR096W TPC1 hom FT MITOCHONDRION -0.000571062475930271 0.973622782090287 2054 Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family thiamine pyrophosphate transport thiamine transmembrane transporter activity integral to membrane|integral to mitochondrial inner membrane YKL189W HYM1 het FT CELL DIVISION|SITE OF POLARIZED GROWTH -0.000572832010846144 0.973537133262306 2055 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response cytokinesis, completion of separation|regulation of cell shape|regulation of transcription, DNA-dependent|budding cell apical bud growth molecular_function incipient cellular bud site|intracellular|cellular bud|mating projection tip YOR380W RDR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000575326921243699 0.973441733272462 2056 Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to xenobiotic stimulus sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YOR269W PAC1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CYTOSKELETON -0.000575423686510726 0.973417450957379 2057 Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly nuclear migration along microtubule microtubule plus-end binding cytoplasmic microtubule|nucleus|cytoplasm YJL122W ALB1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.000575642453395408 0.973407348437805 2058 Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p ribosomal large subunit biogenesis molecular_function ribosome|nucleus|cytoplasm YLL057C JLP1 hom FT OXIDATION-REDUCTION PROCESS -0.000577881877803316 0.973303933447771 2059 Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation sulfur compound catabolic process sulfonate dioxygenase activity cellular_component YBR261C TAE1 hom FT TRANSLATION -0.000579613137521761 0.973223985440788 2060 AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm cytoplasmic translation|N-terminal peptidyl-proline dimethylation N-terminal protein N-methyltransferase activity cytosol|cytoplasm YLR265C NEJ1 hom FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS -0.000580597781827404 0.973178515577784 2061 Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p double-strand break repair via nonhomologous end joining|double-strand break repair via single-strand annealing DNA end binding DNA ligase IV complex|nucleus YML029W USA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX -0.000581395264122505 0.973149695936252 2062 Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p ER-associated protein catabolic process|mRNA splicing, via spliceosome|positive regulation of protein oligomerization protein complex scaffold Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YGL018C JAC1 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.000581607419979901 0.973131891615075 2063 Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix iron-sulfur cluster assembly|aerobic respiration chaperone binding mitochondrion YIL032C_d YIL032C hom -0.000582342820248929 0.973105951952866 2064 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YEL072W RMD6 hom FT CELL CYCLE -0.000583056095286625 0.97306499356708 2065 Protein required for sporulation biological_process molecular_function cellular_component YDL075W RPL31A hom FT TRANSLATION|RIBOSOME -0.000583128230741301 0.973061662445247 2066 Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YGR234W YHB1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION -0.000583268590933059 0.973071249152678 2067 Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress response to stress nitric oxide reductase activity cytosol|mitochondrion|mitochondrial matrix|cytoplasm YPL151C PRP46 het FT CELL CYCLE|RNA PROCESSING|NUCLEUS -0.000585162234604494 0.972971764189482 2068 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs mRNA splicing, via spliceosome molecular_function Prp19 complex|spliceosomal complex YLR105C SEN2 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION -0.000589421140573358 0.972799488107787 2069 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YKL102C_d YKL102C hom -0.000590134380008465 0.972738129796775 2070 Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Unknown Unknown Unknown YNL134C YNL134C hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS -0.000590917110890488 0.972701984827316 2071 Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress biological_process alcohol dehydrogenase (NADP+) activity nucleus|cytoplasm YGR201C_p YGR201C hom -0.000591780175763886 0.972662130257429 2072 Putative protein of unknown function biological_process molecular_function cellular_component YDR258C HSP78 hom FT PROTEIN LOCALIZATION|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000592349160147641 0.972635855760131 2073 Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates positive regulation of mitochondrial translation in response to stress|protein refolding|protein stabilization|protein import into mitochondrial matrix|mitochondrial genome maintenance|protein unfolding|cellular response to heat misfolded protein binding|ATPase activity mitochondrion|mitochondrial matrix YOR378W_p YOR378W hom FT PLASMA MEMBRANE -0.000592759215285649 0.972616920298189 2074 Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene biological_process molecular_function integral to membrane YDL149W ATG9 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|MITOCHONDRION -0.0005944369649405 0.972539445598196 2075 Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS piecemeal microautophagy of nucleus|mitochondrion degradation|protein homooligomerization|CVT pathway|autophagic vacuole assembly molecular_function mitochondrion|integral to membrane|pre-autophagosomal structure YOR292C_p YOR292C hom -0.000594806628748257 0.972522375391002 2076 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene biological_process molecular_function integral to membrane|fungal-type vacuole YDR528W HLR1 hom FT CELL WALL ORG/BIOGENESIS -0.000595499419340864 0.972490383981019 2077 Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication fungal-type cell wall organization molecular_function cytoplasm YDL093W PMT5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000596084289089047 0.972463376131544 2078 Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YIR043C YIR043C hom -0.0005977351751478 0.972387142435639 2079 Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family biological_process molecular_function cellular_component YDR193W_d YDR193W hom -0.000598340529002306 0.972396289599422 2080 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL185W_d YPL185W hom -0.000599238319287611 0.972317731302991 2081 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Unknown Unknown Unknown YOL019W-A_p YOL019W-A hom -0.000600136472459439 0.972296924280545 2082 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPL004C LSP1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|MITOCHONDRION -0.000600934451973339 0.97223940873272 2083 Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family negative regulation of protein kinase activity|endocytosis|response to heat lipid binding mitochondrial outer membrane|eisosome|mitochondrion|plasma membrane|cytoplasm YJL049W_p YJL049W hom FT PROTEIN LOCALIZATION -0.000603971882709838 0.972099149512859 2084 Putative protein of unknown function; YJL049W is a non-essential gene biological_process molecular_function cellular_component YGL245W GUS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION -0.000604033294456662 0.97209631371594 2085 Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress glutamyl-tRNA aminoacylation glutamate-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|mitochondrion|cytoplasm YGL164C YRB30 hom FT PROTEIN LOCALIZATION|NUCLEUS -0.000605305640672718 0.972037560939945 2086 RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes biological_process GTPase regulator activity nucleus|cytoplasm YLR108C YLR108C hom FT NUCLEUS -0.000606177090111323 0.971997320350016 2087 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication biological_process molecular_function nucleus YGL073W HSF1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|CYTOSKELETON ORGANIZATION|NUCLEUS|MITOCHONDRION -0.000606244620585193 0.971994202023931 2088 Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated spindle pole body duplication|negative regulation of TOR signaling cascade|response to heat|regulation of transcription from RNA polymerase II promoter|response to stress sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity mitochondrion|nucleus YEL059W_d HHY1 hom -0.000610186454076421 0.971812182391755 2089 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Unknown Unknown Unknown YLR054C OSW2 hom FT CELL WALL ORG/BIOGENESIS -0.000610652749995904 0.971794855144632 2090 Protein of unknown function proposed to be involved in the assembly of the spore wall ascospore wall assembly molecular_function integral to membrane|prospore membrane|cytoplasm YKL172W EBP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000613010257790065 0.971694454315929 2091 Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering nuclear division|rRNA processing molecular_function nuclear periphery|nucleolus|preribosome, large subunit precursor YPL121C MEI5 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.00061523554386688 0.971579035808279 2092 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p meiotic DNA recombinase assembly|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YKL072W STB6 hom -0.000615975071499395 0.971544887611014 2093 Protein that binds Sin3p in a two-hybrid assay biological_process molecular_function cellular_component YHL016C DUR3 hom FT PLASMA MEMBRANE -0.000616096453491326 0.971539282719 2094 Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway urea transport|spermidine transport|putrescine transport|urea catabolic process urea transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YMR175W SIP18 hom -0.000616140278647855 0.971537259064386 2095 Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress cellular response to water deprivation phospholipid binding cytoplasm YKL129C MYO3 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON -0.00061668739576447 0.97152898346213 2096 One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization bipolar cellular bud site selection|fungal-type cell wall organization|endocytosis|exocytosis|actin cortical patch localization|response to osmotic stress microfilament motor activity actin cortical patch YFL063W_d YFL063W hom -0.000616920539251769 0.971518224342047 2097 Dubious open reading frame, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR133W XPT1 hom FT NUCLEOTIDE METABOLISM -0.000620155379807948 0.971368943255358 2098 Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine XMP salvage|hypoxanthine metabolic process xanthine phosphoribosyltransferase activity|hypoxanthine phosphoribosyltransferase activity cytoplasm YLR023C IZH3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000620694834013588 0.971344048675994 2099 Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity cellular zinc ion homeostasis metal ion binding integral to membrane|endoplasmic reticulum|membrane YDL130W RPP1B hom FT TRANSLATION|RIBOSOME -0.000620787309026246 0.971348335332047 2100 Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component cytoplasmic translation|translational elongation structural constituent of ribosome cytosolic large ribosomal subunit YFR012W DCV1 hom -0.000620869110088451 0.971318903329082 2101 Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YOR046C DBP5 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH|NUCLEAR PORE -0.000621514037571511 0.971289123703646 2102 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p translational termination|mRNA export from nucleus RNA helicase activity|inositol hexakisphosphate binding|RNA-dependent ATPase activity nuclear pore cytoplasmic filaments|nucleus|polysome|nuclear pore|cellular bud tip|cytoplasm YIL165C_p YIL165C hom -0.000622394361213222 0.971248474689075 2103 Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene mitochondrion degradation molecular_function cellular_component YJR127C RSF2 hom FT NUCLEUS -0.000622603282968063 0.971238827721291 2104 Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YOR314W_d YOR314W hom -0.000625275702988386 0.971115429024756 2105 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL166W CUP2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000626322798861858 0.971080018419745 2106 Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter|response to copper ion RNA polymerase II core promoter proximal region sequence-specific DNA binding|copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity|copper ion binding nucleus YGL214W_d YGL214W hom -0.000626946120080498 0.971038298052581 2107 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Unknown Unknown Unknown YIL149C MLP2 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CYTOSKELETON -0.000627211587009459 0.971026040227402 2108 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length poly(A)+ mRNA export from nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|regulation of transcription, DNA-dependent|telomere tethering at nuclear periphery|spindle pole body organization|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery ribonucleoprotein complex binding nucleoplasm|nuclear envelope|mitochondrion|ribonucleoprotein complex|integral to membrane|spindle pole body YPR188C MLC2 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000628522397000904 0.970987157657688 2109 Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring actomyosin contractile ring contraction myosin II binding cellular bud neck contractile ring YER055C HIS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000629032255711477 0.970941971790839 2110 ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control histidine biosynthetic process ATP phosphoribosyltransferase activity cellular_component YMR182W-A_p YMR182W-A hom -0.000630512234572088 0.970873634769418 2111 Putative protein of unknown function biological_process molecular_function cellular_component YDR288W NSE3 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.000630976529914455 0.970886968621553 2112 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress DNA repair DNA binding Smc5-Smc6 complex|nucleus|cytoplasm YML043C RRN11 het FT NUCLEUS -0.000631102661552976 0.970846372263122 2113 Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I CORE element sequence-specific DNA binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity RNA polymerase I core factor complex YNL245C CWC25 het FT RNA PROCESSING|NUCLEUS -0.000634562263727469 0.970686628510777 2114 Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YIL127C_p RRT14 hom FT NUCLEUS -0.000635726315959776 0.970663532023716 2115 Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleolus YBR083W TEC1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000637289463450211 0.97056070355475 2116 Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member pseudohyphal growth|chronological cell aging|positive regulation of transposition, RNA-mediated|invasive growth in response to glucose limitation|positive regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YDR043C NRG1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000638858771488659 0.970488243097321 2117 Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter by glucose|cellular hyperosmotic salinity response|biofilm formation|negative regulation of transcription from RNA polymerase II promoter|pseudohyphal growth|Unknown RNA polymerase II repressing transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nucleus YPL264C_p YPL264C hom -0.000641145710784813 0.970382647523365 2118 Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene biological_process molecular_function integral to membrane YDR277C MTH1 hom FT SIGNALING -0.000642371829844322 0.970352579371395 2119 Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation glucose transport|signal transduction molecular_function cellular_component YDL188C PPH22 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME -0.000644000099142467 0.970268590895598 2120 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication actin filament organization|budding cell bud growth|G1/S transition of mitotic cell cycle|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity condensed nuclear chromosome, centromeric region YPR200C ARR2 hom -0.000644037542983001 0.970253556850395 2121 Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p response to arsenic-containing substance arsenate reductase activity cellular_component YER039C HVG1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS -0.000644993684495608 0.970204974984169 2122 Protein of unknown function, has homology to Vrg4p biological_process molecular_function integral to membrane YJR017C ESS1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEOLYSIS|GENE SILENCING|NUCLEUS -0.000650067237713064 0.969970716007486 2123 Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 termination of RNA polymerase II transcription|positive regulation of protein dephosphorylation|negative regulation of histone deacetylation|negative regulation of transcription from RNA polymerase II promoter|protein peptidyl-prolyl isomerization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription involved in G2/M-phase of mitotic cell cycle peptidyl-prolyl cis-trans isomerase activity|RNA polymerase II core binding nucleus YLR297W YLR297W hom -0.000651833015721505 0.969889186110294 2124 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication biological_process molecular_function fungal-type vacuole YNL128W TEP1 hom FT CELL WALL ORG/BIOGENESIS -0.000653830100581993 0.969801478034036 2125 PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 ascospore wall assembly|phosphatidylinositol dephosphorylation molecular_function cytoplasm YGR084C MRP13 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME -0.000655570615496282 0.969748221537839 2126 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YPL123C RNY1 hom -0.000655597748045294 0.969719874644297 2127 Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity cell morphogenesis|RNA catabolic process|apoptotic process endoribonuclease activity cytosol|extracellular region|vacuole YHR025W THR1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000656225633544287 0.969686370543952 2128 Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway threonine biosynthetic process|methionine metabolic process|isoleucine metabolic process|homoserine metabolic process homoserine kinase activity cellular_component YDR025W RPS11A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME -0.000656770036688105 0.969683849326602 2129 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YDR201W SPC19 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE -0.000656790201364546 0.969660303509724 2130 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|condensed nuclear chromosome kinetochore|DASH complex|spindle pole body|spindle YJL045W YJL045W hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.00065881928745911 0.969566617501312 2131 Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner cellular respiration succinate dehydrogenase (ubiquinone) activity mitochondrion YCR027C RHB1 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE -0.000661063975283345 0.969467528444121 2132 Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins lysine transport|retrograde transport, endosome to Golgi|arginine transport GTPase activity extrinsic to plasma membrane YML086C ALO1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION -0.000661647037401599 0.96943605666197 2133 D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress cellular response to oxidative stress|dehydro-D-arabinono-1,4-lactone biosynthetic process D-arabinono-1,4-lactone oxidase activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YJR112W NNF1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE -0.000661943394698987 0.969422373513035 2134 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore YIR007W_p YIR007W hom FT CARBOHYDRATE METABOLISM -0.000662241486332832 0.969408610296915 2135 Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene biological_process molecular_function cytoplasm YKL188C PXA2 hom FT NUCLEOTIDE METABOLISM -0.000663080843667185 0.969369856301519 2136 Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder fatty acid transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|integral to peroxisomal membrane YDR248C_p YDR248C hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM -0.000663837587843053 0.969334916702983 2137 Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 biological_process molecular_function cytoplasm YOR372C NDD1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS -0.000664220567370886 0.969353835925744 2138 Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes positive regulation of transcription involved in G2/M-phase of mitotic cell cycle transcription coactivator activity nucleus YKL162C-A_d YKL162C-A hom -0.000666235137956619 0.969224219922111 2139 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL092W SSU1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.000667742726531924 0.969182206190482 2140 Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein sulfite transport sulfite transmembrane transporter activity integral to membrane|plasma membrane YLR419W_p YLR419W hom FT MITOCHONDRION -0.000668046540185706 0.969140586444592 2141 Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene biological_process molecular_function mitochondrion|cytoplasm YBR202W MCM7 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME -0.000668287393456078 0.9691294661368 2142 Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex S phase of mitotic cell cycle|regulation of transcription from RNA polymerase II promoter|DNA replication initiation|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|pre-replicative complex assembly|double-strand break repair via break-induced replication|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere DNA replication origin binding|single-stranded DNA-dependent ATPase activity|transcription cofactor activity|single-stranded DNA binding|ATP-dependent 3'-5' DNA helicase activity|ATP binding|four-way junction helicase activity|DNA helicase activity|chromatin binding replication fork protection complex|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YPR111W DBF20 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000668968370541756 0.969098025139713 2143 Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs cytokinesis after mitosis|protein phosphorylation|barrier septum assembly involved in cell cycle cytokinesis|regulation of mRNA catabolic process|exit from mitosis protein serine/threonine kinase activity spindle pole body|cellular bud neck|cytoplasm YPL146C NOP53 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000669277824016069 0.969083737559137 2144 Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired ribosomal large subunit export from nucleus|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA binding nucleolus|nucleus YJR046W TAH11 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS -0.000669509946071138 0.969073020405103 2145 DNA replication licensing factor, required for pre-replication complex assembly regulation of DNA-dependent DNA replication initiation|double-strand break repair via break-induced replication|pre-replicative complex assembly DNA replication origin binding|ATP binding pre-replicative complex|nucleus|cytoplasm YPL229W_p YPL229W hom -0.000670445158219154 0.96902984139952 2146 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication biological_process molecular_function cytoplasm YBR062C_p YBR062C hom -0.000671080495394433 0.969018991816912 2147 Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. biological_process molecular_function cellular_component YNL024C_p YNL024C hom -0.000674033424843678 0.96886417097236 2148 Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern biological_process S-adenosylmethionine-dependent methyltransferase activity cytoplasm YML089C_d YML089C hom -0.000674200585979775 0.968856453168075 2149 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Unknown Unknown Unknown YIL116W HIS5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000677577665796544 0.968719197273296 2150 Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts histidine biosynthetic process histidinol-phosphate transaminase activity intracellular YAL032C PRP45 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -0.000679856758401691 0.968595310268751 2151 Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene generation of catalytic spliceosome for second transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity nucleus|post-mRNA release spliceosomal complex|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YIL058W_d YIL058W hom -0.000682282456767201 0.968483317911957 2152 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YAL028W FRT2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000682602650761513 0.968468534883662 2153 Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication response to stress molecular_function endoplasmic reticulum YBL002W HTB2 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME -0.000683475922849562 0.968428216862133 2154 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation chromatin assembly or disassembly DNA binding replication fork protection complex|nuclear nucleosome YOL163W_p YOL163W hom -0.00068413907594983 0.968397599859382 2155 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YEL002C WBP1 het FT CELL CYCLE|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM -0.000684726440392106 0.968375195746525 2156 Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum cell cycle|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity nuclear envelope|oligosaccharyltransferase complex|endoplasmic reticulum YLL019C KNS1 hom FT PROTEIN PHOSPHORYLATION -0.000685053148322098 0.968355398283748 2157 Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation negative regulation of transcription from RNA polymerase III promoter|protein autophosphorylation|protein phosphorylation protein serine/threonine kinase activity|protein tyrosine kinase activity|protein kinase activity nucleus|cytoplasm YHR192W LNP1 hom -0.000685839302286599 0.968319102611504 2158 Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS endoplasmic reticulum tubular network organization molecular_function endoplasmic reticulum tubular network|cytoplasm YGL255W ZRT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.000685893663988416 0.968340208864468 2159 High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor high-affinity zinc ion transport high affinity zinc uptake transmembrane transporter activity integral to membrane|integral to plasma membrane YFL003C MSH4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME -0.000686140827189197 0.968324079859903 2160 Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein reciprocal meiotic recombination DNA binding nuclear chromosome YPR118W MRI1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS -0.000687172078168855 0.968257570289683 2161 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway L-methionine salvage from methylthioadenosine S-methyl-5-thioribose-1-phosphate isomerase activity nucleus|cytoplasm YML042W CAT2 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION -0.000687584915292274 0.968238510239131 2162 Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes carnitine metabolic process carnitine O-acetyltransferase activity mitochondrion|peroxisomal matrix|peroxisome YOR060C SLD7 het FT CELL CYCLE|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CYTOSKELETON -0.000687827341298788 0.96822731781435 2163 Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria regulation of DNA-dependent DNA replication initiation molecular_function nuclear envelope|endoplasmic reticulum|DNA replication preinitiation complex|nucleus|spindle pole body|cytoplasm YGL128C CWC23 het FT RNA PROCESSING|NUCLEUS -0.000688267315669167 0.968211743025198 2164 Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p spliceosomal complex disassembly molecular_function U2-type spliceosomal complex YDR524C AGE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS -0.000689827843082745 0.968153957674085 2165 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport phospholipid binding|ARF GTPase activator activity endosome|trans-Golgi network|cytoplasm YAL060W BDH1 hom FT OXIDATION-REDUCTION PROCESS -0.00069013580189028 0.968120740158571 2166 NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source alcohol metabolic process|butanediol biosynthetic process (R,R)-butanediol dehydrogenase activity cytoplasm YGL017W ATE1 hom FT PROTEOLYSIS -0.000692112762672435 0.968029467731276 2167 Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway cellular protein modification process|protein arginylation arginyltransferase activity cytoplasm YOL070C NBA1 hom FT SITE OF POLARIZED GROWTH -0.000693139000743394 0.967986860082272 2168 Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate biological_process molecular_function ribosome|cellular bud neck|cytoplasm YKL116C PRR1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN PHOSPHORYLATION -0.000695083184750589 0.967892329919053 2169 Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor negative regulation of transcription from RNA polymerase II promoter by pheromones|negative regulation of conjugation with cellular fusion|protein phosphorylation protein kinase activity|receptor signaling protein serine/threonine kinase activity cytoplasm YEL008W_d YEL008W hom -0.000695470316167849 0.967893611765325 2170 Hypothetical protein predicted to be involved in metabolism Unknown Unknown Unknown YGL058W RAD6 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|GENE SILENCING|NUCLEUS|CHROMOSOME -0.000695975981570187 0.967851111651445 2171 Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p protein ubiquitination involved in ubiquitin-dependent protein catabolic process|histone monoubiquitination|protein monoubiquitination|error-free translesion synthesis|error-prone translesion synthesis|transcription from RNA polymerase II promoter|mitotic cell cycle G1/S transition DNA damage checkpoint|regulation of dipeptide transport|meiotic DNA double-strand break formation|ubiquitin-dependent protein catabolic process via the N-end rule pathway|double-strand break repair via homologous recombination|protein polyubiquitination|error-free postreplication DNA repair|chromatin silencing at telomere ubiquitin-protein ligase activity nuclear chromatin|proteasome complex|nucleus|cytoplasm YER118C SHO1 hom FT SIGNALING|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE -0.000696990900075317 0.967804255425559 2172 Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p signal transduction involved in filamentous growth|establishment of cell polarity|osmosensory signaling pathway|cellular response to heat|cytokinesis osmosensor activity|MAP-kinase scaffold activity integral to membrane|plasma membrane|plasma membrane part|cellular bud|cellular bud neck|mating projection tip YLR112W_d YLR112W hom -0.000697136730611188 0.967797522806897 2173 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR221C RSA3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.000697574094311461 0.967777330869821 2174 Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosomal large subunit assembly molecular_function nucleolus|preribosome, large subunit precursor YKR061W KTR2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS -0.000697591664640585 0.967776519694154 2175 Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication cell wall mannoprotein biosynthetic process|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YBR080C SEC18 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|SITE OF POLARIZED GROWTH -0.000700392075115465 0.967647232568949 2176 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Golgi vesicle docking|SNARE complex disassembly|Golgi to plasma membrane protein transport|vesicle fusion with Golgi apparatus|vacuole fusion, non-autophagic|inter-Golgi cisterna vesicle-mediated transport|autophagic vacuole assembly ATPase activity mating projection tip|cytoplasm YPR156C TPO3 hom FT PLASMA MEMBRANE -0.000702620236621415 0.967554039429236 2177 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication spermine transport spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|plasma membrane YPR199C ARR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000702871980746686 0.967532742966188 2178 Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YML130C ERO1 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000703175062315659 0.967518750669724 2179 Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds protein folding|protein thiol-disulfide exchange thiol oxidase activity endoplasmic reticulum YGR072W UPF3 hom FT NUCLEUS -0.000704989856526334 0.967464096849194 2180 Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|translational frameshifting|protein ubiquitination molecular_function nucleolus|nucleus|polysome|cytoplasm YBL028C YBL028C hom FT NUCLEUS -0.000705856278011563 0.967394968042718 2181 Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function ribosome|nucleolus|nucleus|preribosome, large subunit precursor YJR131W MNS1 hom FT CARBOHYDRATE METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000705966871129018 0.967389862350748 2182 Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation ER-associated protein catabolic process|protein deglycosylation involved in glycoprotein catabolic process mannosyl-oligosaccharide 1,2-alpha-mannosidase activity endoplasmic reticulum YLR003C CMS1 hom FT NUCLEUS -0.000706643808831548 0.96735861056718 2183 Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process molecular_function nucleus|90S preribosome YIL118W RHO3 het FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE -0.000706811163940299 0.967350884390325 2184 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p positive regulation of formin-nucleated actin cable assembly|establishment or maintenance of actin cytoskeleton polarity|positive regulation of exocytosis GTP binding|GTPase activity cytosol|plasma membrane|cellular bud YHR050W-A_p YHR050W-A hom -0.000707001590821387 0.967342093077359 2185 Protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YGL259W_p YPS5 hom FT PROTEOLYSIS -0.000707111564815989 0.967337015981862 2186 Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p biological_process aspartic-type endopeptidase activity cellular_component YAL062W GDH3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS|MITOCHONDRION -0.000707255520018064 0.967330370100797 2187 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication glutamate biosynthetic process|ammonia assimilation cycle glutamate dehydrogenase (NADP+) activity mitochondrion|nucleus YLL034C RIX7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.000707768407926511 0.967306691974994 2188 Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus ATPase activity nucleolus|nucleus|preribosome, large subunit precursor YLR210W CLB4 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|CYTOSKELETON -0.000709024923032909 0.967253564246279 2189 B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation G2/M transition of mitotic cell cycle|S phase of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation cyclin-dependent protein kinase regulator activity nucleus|spindle pole body|cytoplasm YGR185C TYS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|NUCLEUS -0.000709029121301583 0.967263134253659 2190 Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress tyrosyl-tRNA aminoacylation tyrosine-tRNA ligase activity nucleus|cytoplasm YOR283W YOR283W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS -0.000710785786782722 0.967167391407693 2191 Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene dephosphorylation phosphatase activity nucleus|cytoplasm YPR190C RPC82 het FT NUCLEUS -0.00071173854886103 0.967128305744539 2192 RNA polymerase III subunit C82 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YOL153C YOL153C hom -0.000711829046765257 0.967119228411931 2193 Hypothetical protein biological_process molecular_function cellular_component YDR254W CHL4 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE -0.000714142696092926 0.967012417225752 2194 Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 kinetochore assembly|chromosome segregation|establishment of meiotic sister chromatid cohesion|protein localization to chromosome, centromeric region|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion structural molecule activity condensed nuclear chromosome outer kinetochore YGL037C PNC1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|GENE SILENCING|NUCLEUS -0.000714200374900477 0.967009754452137 2195 Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress replicative cell aging|chromatin silencing at rDNA|nicotinate nucleotide salvage|chromatin silencing at telomere nicotinamidase activity nucleus|peroxisome|cytoplasm YGR044C RME1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|NUCLEUS -0.000714497333163474 0.966996045217751 2196 Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription during meiosis|negative regulation of meiosis|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YDR340W_d YDR340W hom -0.000714599416173456 0.96699133250416 2197 Putative protein of unknown function Unknown Unknown Unknown YDR056C_p YDR056C hom FT ENDOPLASMIC RETICULUM -0.000714635319760087 0.966989674997267 2198 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein biological_process molecular_function endoplasmic reticulum YJR021C REC107 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME -0.000715174070652284 0.966964803312108 2199 Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation reciprocal meiotic recombination molecular_function condensed nuclear chromosome YCL044C MGR1 hom FT PROTEOLYSIS|MITOCHONDRION -0.000717951758443182 0.96687612823088 2200 Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA misfolded or incompletely synthesized protein catabolic process misfolded protein binding mitochondrion|i-AAA complex YBR262C AIM5 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000719805369449608 0.966750998579621 2201 Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YCR060W TAH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS -0.000719844665405364 0.966749184485036 2202 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|box C/D snoRNP assembly chaperone binding R2TP complex|nucleus|cytoplasm YDR498C SEC20 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000722134941835229 0.966653396832098 2203 Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p retrograde vesicle-mediated transport, Golgi to ER|vesicle fusion with endoplasmic reticulum SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum|peroxisome YHR093W_d AHT1 hom -0.000722906153033128 0.966607851688452 2204 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Unknown Unknown Unknown YPR155C NCA2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -0.000723510833373226 0.966579936898416 2205 Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p mRNA metabolic process|aerobic respiration molecular_function mitochondrial outer membrane|mitochondrion YDR488C PAC11 hom FT CYTOSKELETON -0.000724266614131393 0.966545046684576 2206 Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 nuclear migration along microtubule|microtubule-based process plus-end-directed microtubule motor activity|microtubule motor activity cytoplasmic dynein complex|cytoplasmic microtubule YOR090C PTC5 hom FT MITOCHONDRION -0.000724745757064779 0.966542887037253 2207 Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p positive regulation of catalytic activity|regulation of catalytic activity|protein dephosphorylation [pyruvate dehydrogenase (lipoamide)] phosphatase activity|protein serine/threonine phosphatase activity mitochondrion YMR042W ARG80 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS -0.000725448723159677 0.966570463282188 2208 Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding nucleus YMR094W CTF13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME|KINETOCHORE -0.000727209773945241 0.966409177899052 2209 Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis kinetochore assembly|septin ring assembly DNA binding, bending|centromeric DNA binding condensed nuclear chromosome kinetochore|CBF3 complex YCR072C RSA4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.00072845151030242 0.966351854355105 2210 WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus ribosomal large subunit assembly molecular_function ribosome|nucleolus YOR308C SNU66 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000729676239407735 0.96629531610455 2211 Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 maturation of 5S rRNA|mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YDR534C FIT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS -0.000731659264144941 0.96620377234276 2212 Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall siderophore transport molecular_function fungal-type cell wall YOR084W LPX1 hom FT PEROXISOME ORGANIZATION -0.000731939930082207 0.966210973272107 2213 Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction peroxisome organization lipase activity peroxisomal matrix YBR205W KTR3 hom FT CARBOHYDRATE METABOLISM -0.000732294842697841 0.966179472455428 2214 Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress N-glycan processing|protein O-linked glycosylation mannosyltransferase activity fungal-type vacuole YGL138C_p YGL138C hom -0.000732422127465463 0.966168555866754 2215 Putative protein of unknown function; has no significant sequence similarity to any known protein biological_process molecular_function cellular_component YOL011W PLB3 hom FT LIPID METABOLISM|PLASMA MEMBRANE -0.000733626542389997 0.966118005764376 2216 Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro phosphatidylinositol metabolic process|phosphatidylserine catabolic process lysophospholipase activity extracellular region|plasma membrane YNL106C INP52 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000737847968409801 0.965943483467468 2217 Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity mating projection tip|cytoplasm|actin cortical patch YHL010C ETP1 hom -0.000737992121667365 0.965911427186329 2218 Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences response to ethanol ubiquitin binding|nuclear localization sequence binding cellular_component YOL031C SIL1 hom FT PROTEIN LOCALIZATION|ENDOPLASMIC RETICULUM -0.00073849967414783 0.96589816450107 2219 Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein SRP-dependent cotranslational protein targeting to membrane, translocation adenyl-nucleotide exchange factor activity endoplasmic reticulum YEL056W HAT2 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|HISTONE ACETYLTRANSFERASE -0.000739612168831197 0.965836641311077 2220 Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing histone acetylation|chromatin assembly or disassembly|chromatin silencing at telomere histone acetyltransferase activity|histone binding histone acetyltransferase complex|nucleus|cytoplasm YLR389C STE23 hom FT PROTEOLYSIS -0.000740144457158166 0.965812069469496 2221 Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family peptide pheromone maturation metallopeptidase activity|metalloendopeptidase activity extrinsic to membrane YLR365W_d YLR365W hom -0.000740782469015145 0.96578771618587 2222 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Unknown Unknown Unknown YDR311W TFB1 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME -0.000741027657969953 0.965771298648083 2223 Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase I promoter|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-5-phosphate binding core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YOL130W ALR1 het FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE -0.000741279680355764 0.965759664662851 2224 Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions magnesium ion transport|cation transport inorganic cation transmembrane transporter activity integral to membrane|plasma membrane YML098W TAF13 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME -0.000741490986115572 0.96574991026478 2225 TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YLR152C_p YLR152C hom -0.000742130735164401 0.9657510388928 2226 Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function integral to membrane YPR152C URN1 hom FT RNA PROCESSING|NUCLEUS -0.000743501342813677 0.965657107468821 2227 Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase biological_process molecular_function nucleus YMR147W_p YMR147W hom -0.000743791618486783 0.965643707699058 2228 Putative protein of unknown function biological_process molecular_function cellular_component YKL106C-A_p YKL106C-A hom -0.000743922721058079 0.965678641712332 2229 Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi biological_process molecular_function cellular_component YPL231W FAS2 het FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION -0.000746735291171204 0.965507821778622 2230 Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities fatty acid biosynthetic process holo-[acyl-carrier-protein] synthase activity|3-oxoacyl-[acyl-carrier-protein] synthase activity|fatty acid synthase activity|3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity cytosol|fatty acid synthase complex|mitochondrion|cytoplasm YDR198C RKM2 hom -0.000746807121414071 0.96550450596096 2231 Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp peptidyl-lysine trimethylation|peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity cellular_component YJL211C_d YJL211C hom -0.000749660256408688 0.965403771921751 2232 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Unknown Unknown Unknown YDR522C SPS2 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE -0.000750735301205897 0.965323174782213 2233 Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component ascospore wall assembly|ascospore formation molecular_function fungal-type cell wall|plasma membrane YBR011C IPP1 het -0.000751577126358646 0.965289488151167 2234 Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase phosphate-containing compound metabolic process inorganic diphosphatase activity|protein homodimerization activity cytosol YGL230C_p YGL230C hom -0.000751894827127756 0.965269649518853 2235 Putative protein of unknown function; non-essential gene biological_process molecular_function cellular_component YBR235W VHC1 hom -0.000753483936048656 0.965196294385327 2236 Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family transmembrane transport ion transmembrane transporter activity integral to membrane YGL262W_p YGL262W hom -0.000754733260795016 0.965138624294917 2237 Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene biological_process molecular_function cellular_component YKL099C UTP11 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000754758685454323 0.965137450668891 2238 Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YJL103C GSM1 hom FT TRANSCRIPTION FROM RNA POL II|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS -0.000754850362297239 0.9651332187808 2239 Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis oxidative phosphorylation sequence-specific DNA binding|DNA binding cellular_component YOR004W UTP23 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION -0.000760881631952757 0.964854811942348 2240 Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|mitochondrion|nucleolus YNL304W YPT11 hom FT SIGNALING|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH -0.00076159327380799 0.964821962431272 2241 Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells mitochondrion inheritance|endoplasmic reticulum inheritance|Golgi inheritance|Golgi localization GTPase activity incipient cellular bud site|endoplasmic reticulum|cellular bud neck|cellular bud tip YJL051W IRC8 hom -0.000761878181291984 0.964829791462184 2242 Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc mitotic recombination molecular_function integral to membrane|cellular bud YDR154C_d YDR154C hom -0.000762206932284668 0.964798882078526 2243 Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YHR116W COX23 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000762527623268204 0.964784081172964 2244 Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex assembly molecular_function mitochondrion|cytoplasm YOR334W MRS2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION -0.000762937696476801 0.964759903811351 2245 Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YBR071W YBR071W hom FT SITE OF POLARIZED GROWTH -0.000764896236760831 0.964674762527121 2246 Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking biological_process molecular_function cellular bud neck|cytoplasm YJL144W YJL144W hom -0.000765845521084329 0.964625679207401 2247 Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress cellular response to water deprivation molecular_function cytoplasm YIL028W_d YIL028W hom -0.000766645439921914 0.964588755288958 2248 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR212W NGR1 hom FT MITOCHONDRION ORGANIZATION -0.000766998772793121 0.964572445615803 2249 RNA binding protein that neg