Strain Gene Zyg FClass FTerms similarity score p-value rank description GOBP GOMF GOCC YGR202C PCT1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 1 0 1 Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity phosphatidylcholine biosynthetic process|CDP-choline pathway choline-phosphate cytidylyltransferase activity nuclear envelope|Golgi apparatus|nucleus YGL077C HNM1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.405119820512943 1.20625784647381e-132 2 Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol choline transport|ethanolamine transport|glycine betaine transport choline transmembrane transporter activity|ethanolamine transmembrane transporter activity integral to membrane|plasma membrane YPR029C APL4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.388109174095721 5.65733189618742e-121 3 Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YHL019C APM2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.354062774491779 1.33051306907376e-99 4 Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YLR169W_d YLR169W hom 0.342653149881471 5.26904971133748e-93 5 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR115C TRS33 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.329142537819343 1.5276130482719e-85 6 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport|protein complex assembly molecular_function trans-Golgi network|TRAPP complex|cis-Golgi network YLR170C APS1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.316024065267477 1.19092962904789e-78 7 Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YNL183C NPR1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|GOLGI APPARATUS|PLASMA MEMBRANE 0.31022905468111 1.0274525660361e-75 8 Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex regulation of transmembrane transporter activity|regulation of nitrogen utilization|protein phosphorylation|negative regulation of endocytosis protein serine/threonine kinase activity Golgi apparatus|plasma membrane|cytoplasm YGR166W TRS65 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|GOLGI APPARATUS 0.310187380827681 1.07804460585548e-75 9 Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response intra-Golgi vesicle-mediated transport|protein complex assembly Rab guanyl-nucleotide exchange factor activity TRAPP complex|trans-Golgi network YJL154C VPS35 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.309435620475146 2.56225242901391e-75 10 Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p protein retention in Golgi apparatus|retrograde transport, endosome to Golgi protein transporter activity endosome|retromer complex, inner shell|retromer complex YML037C_p YML037C hom 0.30835126540872 9.34918461740959e-75 11 Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene biological_process molecular_function clathrin-coated vesicle YJL052W TDH1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.306036794592913 1.37292008197095e-73 12 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress gluconeogenesis|glycolysis glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YBL010C_p YBL010C hom FT NUCLEUS 0.306023801964329 1.26244087201441e-73 13 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles biological_process molecular_function clathrin-coated vesicle YKL191W DPH2 hom 0.303230341277642 2.95308104933104e-72 14 Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YLR020C YEH2 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.303029046129543 3.70125387013106e-72 15 Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism cell wall mannoprotein biosynthetic process|sterol metabolic process sterol esterase activity integral to membrane|plasma membrane YOR357C SNX3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.300598041718072 5.57986967849617e-71 16 Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p protein localization|late endosome to Golgi transport phosphatidylinositol-3-phosphate binding cytosol|endosome YNL267W PIK1 het FF|FT LIPID METABOLISM|SIGNALING|NUCLEUS|GOLGI APPARATUS 0.30026013404074 1.36741113980949e-70 17 Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity trans-Golgi network|nucleus YBR245C ISW1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.300030854780396 1.04689577044524e-70 18 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions termination of RNA polymerase I transcription|nucleosome positioning|termination of RNA polymerase II transcription|regulation of transcription by chromatin organization|DNA-dependent transcription, elongation|regulation of transcriptional start site selection at RNA polymerase II promoter|heterochromatin maintenance involved in chromatin silencing|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter nucleosome binding|ATPase activity|rDNA binding|DNA binding ISW1 complex|nucleolar chromatin YBR246W RRT2 hom FT VESICLE-MEDIATED TRANSPORT 0.282641630758005 1.32185541699606e-62 19 Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-diphthamide biosynthetic process from peptidyl-histidine|endocytic recycling molecular_function endosome|cytoplasm YHR108W GGA2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.275119523631132 2.66142704396319e-59 20 Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to vacuole transport|Golgi to endosome transport ubiquitin binding|phosphatidylinositol-4-phosphate binding trans-Golgi network YMR126C_p DLT1 hom 0.271472609876248 1.03548115418505e-57 21 Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) biological_process molecular_function cellular_component YOR039W CKB2 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.269298725492406 1.01594875773085e-56 22 Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase inhibitor activity|protein kinase regulator activity protein kinase CK2 complex|UTP-C complex|CURI complex YFL034W_p YFL034W hom 0.268711387757977 1.66970016742706e-56 23 Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk biological_process molecular_function integral to membrane YNL295W_p YNL295W hom 0.265968066513381 3.43141075806187e-55 24 Putative protein of unknown function biological_process molecular_function cellular_component YML106W URA5 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.263421581318134 2.43516691250898e-54 25 Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine ribonucleoside biosynthetic process orotate phosphoribosyltransferase activity nucleus|cytoplasm YBL082C ALG3 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.26306307376677 3.42520327013415e-54 26 Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation alpha-1,3-mannosyltransferase activity integral to membrane|endoplasmic reticulum YJL124C LSM1 hom FF|FT RNA PROCESSING|NUCLEUS 0.249951339017466 8.37444538376987e-49 27 Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay RNA binding|RNA cap binding mRNA cap binding complex|cytoplasmic mRNA processing body|cytoplasm YHR012W VPS29 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.247623519298293 4.99755149870087e-48 28 Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval retrograde transport, endosome to Golgi protein transporter activity endosome|retromer complex, inner shell|retromer complex YDR159W SAC3 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.245837495981126 3.11574994247524e-47 29 Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) ribosomal small subunit biogenesis|actin filament-based process|mRNA export from nucleus|mRNA 3'-end processing|mitosis|protein export from nucleus|transcription-coupled nucleotide-excision repair|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery protein binding transcription export complex 2|nuclear pore YPR153W YPR153W hom 0.244621088176903 7.05968064149128e-47 30 Putative protein of unknown function biological_process molecular_function integral to membrane YCR095C_p OCA4 hom 0.244417073593982 8.44033993990737e-47 31 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm YGL019W CKB1 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.244334144007031 1.02706012336491e-46 32 Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein kinase regulator activity|protein serine/threonine kinase inhibitor activity protein kinase CK2 complex|UTP-C complex YPL205C_d YPL205C hom 0.238079697308705 1.99616198319278e-44 33 Hypothetical protein; deletion of locus affects telomere length Unknown Unknown Unknown YDR455C_d YDR455C hom FF 0.232321151870485 2.49612853999478e-42 34 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Unknown Unknown Unknown YAL009W SPO7 hom FF|FT CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.229603889229844 2.39305255483582e-41 35 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation negative regulation of phospholipid biosynthetic process|nuclear envelope organization phosphoprotein phosphatase activity Nem1-Spo7 phosphatase complex|integral to membrane YNL079C TPM1 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.228558526190139 5.45632152228524e-41 36 Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication vacuole inheritance|exocytosis|intracellular mRNA localization|filamentous growth|budding cell apical bud growth|mitochondrion inheritance|budding cell isotropic bud growth|actin polymerization or depolymerization|actin filament organization|pseudohyphal growth|vesicle-mediated transport|establishment of cell polarity|actin filament bundle assembly actin lateral binding cellular bud neck contractile ring|actin filament bundle YJL053W PEP8 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.225166504256601 8.3944332018506e-40 37 Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress protein retention in Golgi apparatus|retrograde transport, endosome to Golgi protein transporter activity endosome|retromer complex, inner shell|retromer complex YGL199C_d YGL199C hom 0.220583563321114 3.14068264890644e-38 38 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Unknown Unknown Unknown YOL159C-A YOL159C-A hom FT NUCLEUS 0.220562466045303 3.71145725092305e-38 39 Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function nucleus|cytoplasm YCR075W-A_p YCR075W-A hom 0.21905894650825 1.02921532197305e-37 40 Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YDR508C GNP1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.215491103123016 1.59816639222539e-36 41 High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity|L-proline transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YGL161C YIP5 hom FT GOLGI APPARATUS 0.215102875035153 2.14758262892193e-36 42 Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process Rab GTPase binding integral to membrane|Golgi apparatus YDL090C RAM1 hom FF|FT PROTEIN PRENYLATION 0.215047672890709 3.69352210647086e-36 43 Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit protein farnesylation protein farnesyltransferase activity protein farnesyltransferase complex YPR091C NVJ2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.214627629840439 3.08105515393437e-36 44 Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding ribosome|nucleus-vacuole junction|endoplasmic reticulum YML097C VPS9 hom FF|FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.21321247810456 8.97909899069997e-36 45 A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin vacuole inheritance|protein targeting to vacuole ubiquitin binding|guanyl-nucleotide exchange factor activity cytosol YOL072W THP1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.211427840008801 3.42224988106785e-35 46 Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding mRNA export from nucleus|mRNA 3'-end processing|transcription-coupled nucleotide-excision repair|cellular bud site selection|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|transcription elongation from RNA polymerase II promoter double-stranded DNA binding|RNA binding nuclear envelope|nucleus|transcription export complex 2|nuclear pore YDR084C TVP23 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.209839219122079 1.11462336412164e-34 47 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|cytoplasm YDR317W HIM1 hom FT RESPONSE TO DNA DAMAGE 0.209152471880889 1.8514935451539e-34 48 Protein of unknown function involved in DNA repair DNA repair molecular_function cellular_component YJR105W ADO1 hom FF|FT NUCLEOTIDE METABOLISM|NUCLEUS 0.208482072742311 3.7936232127212e-34 49 Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle purine nucleobase metabolic process adenosine kinase activity nucleus|cytoplasm YKR072C SIS2 hom FF|FT CELL CYCLE|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.207134324237365 8.89274099979882e-34 50 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis regulation of mitotic cell cycle|response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex|nucleus|cytoplasm YJL204C RCY1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.206877870523212 9.81610283841847e-34 51 F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth early endosome to Golgi transport|endocytosis SNARE binding endosome|Golgi apparatus|site of polarized growth YCL046W_d YCL046W hom 0.206717443737623 1.23153369292346e-33 52 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Unknown Unknown Unknown YLR133W CKI1 hom FT LIPID METABOLISM 0.206483159678489 1.30851520303745e-33 53 Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolamine kinase activity|choline kinase activity cytoplasm YNL077W APJ1 hom FT NUCLEUS|MITOCHONDRION 0.206430242516516 1.35986560413695e-33 54 Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress biological_process unfolded protein binding mitochondrion|nucleus|cytoplasm YLR360W VPS38 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.205462421156424 2.74429474299697e-33 55 Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated ER-associated protein catabolic process|late endosome to vacuole transport molecular_function phosphatidylinositol 3-kinase complex II YPL077C_p YPL077C hom 0.20462391478341 5.02778935082926e-33 56 Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL120W VPS30 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.204399810522663 6.03655728686469e-33 57 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 phosphatidylinositol biosynthetic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|peroxisome degradation|retrograde transport, endosome to Golgi|macroautophagy|CVT pathway|late endosome to vacuole transport 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|extrinsic to membrane|cytoplasm YIL048W NEO1 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.20220621517012 2.83846217475449e-32 58 Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus retrograde vesicle-mediated transport, Golgi to ER|vacuole organization|endocytosis phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism endosome|COPI-coated vesicle|integral to membrane|Golgi apparatus|Golgi membrane YBR295W PCA1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.200109299416139 1.35849182204743e-31 59 Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function cadmium ion transport|cellular iron ion homeostasis|cellular metal ion homeostasis|transmembrane transport copper-transporting ATPase activity|cadmium-exporting ATPase activity|copper ion binding|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism integral to membrane|plasma membrane YKR006C MRPL13 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.198414233583296 4.100354417212e-31 60 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YDR372C VPS74 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.198269198334188 5.01851766495434e-31 61 Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 retrograde vesicle-mediated transport, Golgi to ER|endoplasmic reticulum unfolded protein response|protein localization to Golgi apparatus enzyme binding|phosphatidylinositol-4-phosphate binding cytosol|Golgi apparatus|nucleus|extrinsic to membrane|cytoplasm YLR057W MNL2 hom FT PROTEOLYSIS|ENDOPLASMIC RETICULUM 0.19791975200348 5.78528932602691e-31 62 Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene ER-associated protein catabolic process molecular_function endoplasmic reticulum YBL022C PIM1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.197727269842352 6.61322577107578e-31 63 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria chaperone-mediated protein complex assembly|misfolded or incompletely synthesized protein catabolic process ATP-dependent peptidase activity mitochondrion|mitochondrial matrix YER177W BMH1 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.193896863182166 9.20144532328198e-30 64 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|pseudohyphal growth|signal transduction involved in filamentous growth|ascospore formation|fungal-type cell wall chitin biosynthetic process|Ras protein signal transduction|DNA damage checkpoint|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle DNA replication origin binding|phosphoserine binding plasma membrane|nucleus YDR378C LSM6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.192817592035479 2.40566393420927e-29 65 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasmic mRNA processing body YKL135C APL2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.191268493892018 5.42898040995156e-29 66 Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YMR071C TVP18 hom FF|FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.190959023865284 7.06541058778379e-29 67 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|clathrin-coated vesicle YDR156W RPA14 hom FT NUCLEUS 0.190877504216901 7.0540129644622e-29 68 RNA polymerase I subunit A14 transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YCR079W PTC6 hom FT RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.189996505263649 1.2698955706586e-28 69 Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase mitochondrion degradation|positive regulation of catalytic activity|macroautophagy|protein dephosphorylation protein serine/threonine phosphatase activity mitochondrion|mitochondrial matrix|mitochondrial intermembrane space YHL020C OPI1 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|SIGNALING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.189694428937546 1.55248699921343e-28 70 Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance endoplasmic reticulum unfolded protein response|negative regulation of transcription from RNA polymerase II promoter|phospholipid biosynthetic process|positive regulation of transcription from RNA polymerase II promoter transcription corepressor activity nuclear membrane|nucleoplasm|nuclear envelope|endoplasmic reticulum|nucleus YLR025W SNF7 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.188403087312938 3.65088123700594e-28 71 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes cellular response to anoxia|intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport protein binding ESCRT III complex|cytoplasm YDR492W IZH1 hom FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.186844240427256 1.016542094528e-27 72 Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc response to zinc ion|cellular zinc ion homeostasis|cellular lipid metabolic process metal ion binding integral to membrane|endoplasmic reticulum|membrane YKR073C_d YKR073C hom 0.18570965519039 2.12990939297079e-27 73 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL098W MTC2 hom 0.185389440672846 2.8637915170863e-27 74 Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YCR077C PAT1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|TRANSLATION|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|RIBOSOME 0.185063870390991 3.35384543200623e-27 75 Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|chromosome segregation|regulation of translational initiation|formation of translation preinitiation complex|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly molecular_function cytosolic small ribosomal subunit|cytoplasmic mRNA processing body YGR178C PBP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS|MITOCHONDRION 0.183623692978005 8.19555576417651e-27 76 Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress positive regulation of translation|mRNA polyadenylation|stress granule assembly molecular_function mitochondrion|nucleus|polysome|cytoplasmic stress granule|cytoplasm YLR095C IOC2 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.182018626018325 2.2863517366437e-26 77 Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif chromatin remodeling protein binding|nucleosome binding|ATPase activity|DNA binding ISW1 complex YNL056W OCA2 hom 0.181392964386159 3.40182395944758e-26 78 Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene biological_process molecular_function cytoplasm YKL176C LST4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.180292967828628 6.8170662547356e-26 79 Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface intracellular protein transport|vesicle-mediated transport|protein transport|Golgi to plasma membrane transport protein transporter activity vesicle coat YJR102C VPS25 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.179888269667503 9.24270466892588e-26 80 Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome negative regulation of transcription from RNA polymerase II promoter by glucose|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole structural molecule activity ESCRT II complex YNL070W TOM7 hom FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.179608616598708 1.04818984992364e-25 81 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex protein import into mitochondrial matrix|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YLR171W_d YLR171W hom 0.178422050842989 2.42753254717657e-25 82 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL065W VPS28 hom FF|FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.177498016415652 3.90829800505087e-25 83 Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole protein complex binding endosome|ESCRT I complex YOL089C HAL9 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.175764156950457 1.13821212377911e-24 84 Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication response to salt stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding mitochondrion|nucleus YPL244C HUT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.17418458833044 3.12797957038945e-24 85 Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 UDP-galactose transport|UDP-glucose transport UDP-galactose transmembrane transporter activity integral to membrane YPL202C AFT2 hom FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.172865843568769 6.73379533226995e-24 86 Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication cellular response to oxidative stress|cellular iron ion homeostasis|positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription mitochondrion|nucleus YML036W CGI121 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|CHROMOSOME 0.171744981733626 1.36063441039379e-23 87 Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification telomere maintenance|positive regulation of transcription from RNA polymerase II promoter molecular_function EKC/KEOPS complex YDR101C ARX1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|PROTEOLYSIS|NUCLEUS|RIBOSOME 0.171733498044858 1.32921858427772e-23 88 Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex ribosomal large subunit biogenesis molecular_function nucleoplasm|cytosolic large ribosomal subunit|cytoplasm YOL144W NOP8 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.17160204192791 1.5274529152484e-23 89 Nucleolar protein required for 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus YML072C TCB3 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.170787901062646 2.30242265312445e-23 90 Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding mitochondrion|cortical endoplasmic reticulum|cellular bud YHR004C NEM1 hom FF|FT LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.170168734533911 3.74542577825653e-23 91 Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard negative regulation of phospholipid biosynthetic process|nuclear envelope organization phosphoprotein phosphatase activity Nem1-Spo7 phosphatase complex|mitochondrion|integral to membrane YML130C ERO1 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.170026085438755 3.61965127786542e-23 92 Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds protein folding|protein thiol-disulfide exchange thiol oxidase activity endoplasmic reticulum YKR095W MLP1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.169777035907003 4.19470628309092e-23 93 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs protein localization to nuclear pore|poly(A)+ mRNA export from nucleus|protein import into nucleus|transcriptional activation by promoter-terminator looping|nuclear retention of unspliced pre-mRNA at the site of transcription|telomere tethering at nuclear periphery ribonucleoprotein complex binding nucleoplasm|nuclear envelope|integral to membrane|ribonucleoprotein complex|spindle pole body YDL045C FAD1 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.169407586447125 5.2181130998417e-23 94 Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin FAD biosynthetic process FMN adenylyltransferase activity cytoplasm YDR334W SWR1 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.168908071951708 7.00426817467805e-23 95 Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin remodeling|histone exchange structural molecule activity Swr1 complex|nucleus YBL083C_d YBL083C hom 0.166968864659064 2.17762344064034e-22 96 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Unknown Unknown Unknown YFL023W BUD27 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RIBOSOME 0.166352024361947 3.53882828776968e-22 97 Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP formation of translation preinitiation complex molecular_function cytosolic ribosome|cytoplasm YPR173C VPS4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.166026510200063 3.76045642880698e-22 98 AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism late endosome to vacuole transport via multivesicular body sorting pathway|protein retention in Golgi apparatus|intralumenal vesicle formation|protein homooligomerization|sterol metabolic process|late endosome to vacuole transport ATPase activity|protein homodimerization activity|ATP binding endosome|endoplasmic reticulum|membrane|cytoplasm YML094C-A_d YML094C-A hom FF 0.16463943767898 8.35478110948185e-22 99 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Unknown Unknown Unknown YER120W SCS2 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.16455231640129 8.78234061825702e-22 100 Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication endoplasmic reticulum inheritance|regulation of intracellular lipid transport|negative regulation of transcription factor import into nucleus|phospholipid biosynthetic process|chromatin silencing at telomere phosphatidylinositol binding|FFAT motif binding nuclear membrane|integral to endoplasmic reticulum membrane|nuclear envelope|endoplasmic reticulum|cellular bud tip YKL190W CNB1 hom FF|FT SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS 0.162540351449941 2.75977471739454e-21 101 Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|fungal-type cell wall organization|cellular ion homeostasis calcium-dependent protein serine/threonine phosphatase activity|calcium ion binding calcineurin complex YJL179W PFD1 hom FT CYTOSKELETON ORGANIZATION 0.162432775753788 2.9328055113504e-21 102 Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin protein folding|cytoskeleton organization unfolded protein binding prefoldin complex YOR089C VPS21 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|MITOCHONDRION 0.161624129762614 4.6262219470201e-21 103 Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication endocytosis|vacuole inheritance|protein targeting to vacuole GTP binding|GTPase activity mitochondrial outer membrane|late endosome|mitochondrion YHL034C SBP1 hom FT TRANSLATION|NUCLEUS 0.16139757017524 5.25414025534125e-21 104 Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 negative regulation of translation|negative regulation of translation in response to stress RNA binding|eukaryotic initiation factor 4G binding nucleolus|cytoplasmic mRNA processing body|cytoplasm YDR389W SAC7 hom FF|FT CELL CYCLE|SIGNALING|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.161394023904445 5.26461033824363e-21 105 GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate actin filament reorganization involved in cell cycle|small GTPase mediated signal transduction Rho GTPase activator activity intracellular YDL033C SLM3 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.160744144886689 7.88348054958183e-21 106 tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) mitochondrial tRNA thio-modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity mitochondrion YAR042W SWH1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.159965465152932 1.16963744019303e-20 107 Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction endocytosis|maintenance of cell polarity|exocytosis|sterol transport lipid binding|oxysterol binding|1-phosphatidylinositol binding|sterol transporter activity nuclear envelope|endoplasmic reticulum|Golgi trans cisterna|early endosome YMR230W-A_p YMR230W-A hom 0.159292401169842 1.69938069926716e-20 108 Putative protein of unknown function biological_process molecular_function cellular_component YGL079W_p KXD1 hom 0.156716053283986 6.99527805605638e-20 109 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome biological_process molecular_function endosome YJR087W_d YJR087W hom 0.155710691800789 1.20729092051065e-19 110 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Unknown Unknown Unknown YLR087C CSF1 hom FF|FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.155509469368943 1.39700587814072e-19 111 Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies fermentation molecular_function mitochondrion YBR296C PHO89 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.155121949219985 1.65911674618369e-19 112 Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification phosphate ion transport|transmembrane transport sodium:inorganic phosphate symporter activity integral to membrane|plasma membrane YGL072C_d YGL072C hom FF 0.155066056241197 1.70984373722126e-19 113 Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YDR491C_d YDR491C hom 0.154004091293096 3.02376244659749e-19 114 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR097W JJJ3 hom FT NUCLEUS 0.153911936781337 3.17651373743324e-19 115 Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 peptidyl-diphthamide biosynthetic process from peptidyl-histidine ferrous iron binding nucleus|cytoplasm YIL052C RPL34B hom FF|FT TRANSLATION|RIBOSOME 0.153198255057599 4.64793780741477e-19 116 Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJL207C LAA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.15086848227624 1.74555841834241e-18 117 AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene protein localization|retrograde transport, endosome to Golgi molecular_function AP-1 adaptor complex|ribosome|clathrin-coated vesicle YOR293W RPS10A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.150577646251257 1.98511955777989e-18 118 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YJL081C ARP4 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.150575714866954 2.08160400833787e-18 119 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes DNA repair|histone acetylation|kinetochore assembly|chromatin organization|regulation of transcription, DNA-dependent|regulation of transcription from RNA polymerase II promoter|chromatin remodeling chromatin binding|histone acetyltransferase activity|ATP-dependent 3'-5' DNA helicase activity nuclear chromatin|Swr1 complex|nucleus|Ino80 complex|NuA4 histone acetyltransferase complex YGL045W RIM8 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.150564618148102 1.93023981691102e-18 120 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family meiosis|protein processing|invasive growth in response to glucose limitation|ubiquitin-dependent endocytosis molecular_function internal side of plasma membrane YEL048C TCA17 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|GOLGI APPARATUS 0.149543845704199 3.20912703305293e-18 121 Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder early endosome to Golgi transport|protein complex assembly molecular_function TRAPP complex|Golgi apparatus|clathrin-coated vesicle YHL027W RIM101 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CELL DIVISION|ION HOMEOSTASIS|NUCLEUS 0.148971305768978 4.31690450511211e-18 122 Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC meiosis|negative regulation of transcription from RNA polymerase II promoter|fungal-type cell wall biogenesis|cellular response to anoxia|response to pH|ascospore formation|barrier septum assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding nucleus YPL145C KES1 hom FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.148443867300036 5.60357718430108e-18 123 One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication post-Golgi vesicle-mediated transport|positive regulation of phosphatase activity|maintenance of cell polarity|endocytosis|exocytosis|sterol transport phosphatidic acid binding|lipid binding|phosphatidylinositol-4,5-bisphosphate binding|oxysterol binding|sterol transporter activity|phosphatidylinositol-4-phosphate binding Golgi membrane|extrinsic to membrane|cytoplasm YML041C VPS71 hom FF|FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|VACUOLAR TRANSPORT|NUCLEUS|CHROMOSOME 0.148267472153135 6.71476934577908e-18 124 Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting chromatin remodeling|protein targeting to vacuole|histone exchange nucleosome binding Swr1 complex|nucleus YGL227W VID30 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.147193807682737 1.07594099919187e-17 125 Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm regulation of nitrogen utilization|negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|vacuolar protein catabolic process molecular_function nucleus|GID complex|cytoplasm YDR456W NHX1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|GOLGI APPARATUS 0.147184257845896 1.06926969142541e-17 126 Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion sodium ion transmembrane transport|potassium ion transmembrane transport|cellular monovalent inorganic cation homeostasis|vacuolar acidification sodium:hydrogen antiporter activity|potassium:hydrogen antiporter activity trans-Golgi network|late endosome|fungal-type vacuole membrane|integral to membrane|early endosome YGL035C MIG1 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.144783789301833 3.60691052133173e-17 127 Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion negative regulation of transcription from RNA polymerase II promoter by glucose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|nuclear envelope lumen|cytoplasm YHR007C ERG11 het FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.144684614400162 3.79106869425905e-17 128 Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p ergosterol biosynthetic process sterol 14-demethylase activity endoplasmic reticulum YBL067C UBP13 hom FT PROTEOLYSIS 0.144544939931664 4.06624763683045e-17 129 Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication biological_process ubiquitin-specific protease activity cellular_component YDL133W SRF1 hom 0.143896028765906 5.62582931639793e-17 130 Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients positive regulation of catalytic activity molecular_function integral to membrane YGL043W DST1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS 0.143869493143124 5.70083394926467e-17 131 General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress transcription elongation from RNA polymerase I promoter|transcription initiation from RNA polymerase II promoter|mRNA cleavage|maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter|tRNA transcription from RNA polymerase III promoter|regulation of mRNA 3'-end processing|positive regulation of transcription elongation from RNA polymerase II promoter|transcription from RNA polymerase III promoter|transcription antitermination|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter RNA polymerase II regulatory region DNA binding|RNA polymerase II core binding|core RNA polymerase II recruiting transcription factor activity nucleus YAL002W VPS8 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.143636694989365 6.40255557710903e-17 132 Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif late endosome to vacuole transport via multivesicular body sorting pathway|protein targeting to vacuole GTPase binding|protein anchor late endosome|membrane|CORVET complex YLR216C CPR6 hom 0.142370856985382 1.19959427960485e-16 133 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding|protein refolding unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytoplasm YJR088C EMC2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.14228990540385 1.2485022289263e-16 134 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YDR525W_d API2 hom 0.142149828095147 1.39437520001298e-16 135 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Unknown Unknown Unknown YBL046W PSY4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.140827335759398 2.55989331505564e-16 136 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Unknown|protein dephosphorylation protein phosphatase regulator activity nucleus|cytoplasm YNL205C_d YNL205C hom 0.139969848222739 4.08626237439301e-16 137 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL124C MON1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.139317248281679 5.33044508715548e-16 138 Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate autophagy|vesicle docking|protein targeting to vacuole molecular_function cytosol|fungal-type vacuole membrane|Mon1-Ccz1 complex YDL025C RTK1 hom FT PROTEIN PHOSPHORYLATION 0.138425244177344 8.1901857247834e-16 139 Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress biological_process protein serine/threonine kinase activity|protein kinase activity cellular_component YOL001W PHO80 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.137901427968199 1.05260840982573e-15 140 Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling negative regulation of phosphate metabolic process|regulation of cyclin-dependent protein kinase activity|negative regulation of calcium-mediated signaling|negative regulation of transcription from RNA polymerase II promoter|cellular metal ion homeostasis|regulation of protein localization|negative regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YER180C-A SLO1 hom FT PROTEIN LOCALIZATION 0.13737887410015 1.35070876256027e-15 141 Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO intracellular protein transport small GTPase regulator activity cytoplasm YDR162C NBP2 hom FF|FT PROTEIN PHOSPHORYLATION|NUCLEUS 0.137329229821798 1.50870645831687e-15 142 Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) negative regulation of protein kinase activity|response to heat|hyperosmotic response molecular_function nucleus|cytoplasm YNL294C RIM21 hom FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.137031390512175 1.59347437974291e-15 143 pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH fungal-type cell wall biogenesis|ascospore formation|invasive growth in response to glucose limitation molecular_function integral to membrane YDR467C_d YDR467C hom 0.137000471115132 1.61705036601599e-15 144 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL136W_d YKL136W hom 0.13681736728616 1.76387119558373e-15 145 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Unknown Unknown Unknown YDR028C REG1 hom FF|FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.135546106237735 3.2144718559379e-15 146 Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p cellular response to glucose starvation|vacuolar protein catabolic process|glycogen metabolic process|negative regulation of transcription from RNA polymerase II promoter|regulation of carbohydrate metabolic process protein phosphatase type 1 regulator activity protein phosphatase type 1 complex|cytoplasm YCL008C STP22 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.135217320032046 3.75077182181975e-15 147 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT I complex|internal side of plasma membrane YBR255W MTC4 hom 0.135205761114673 3.7711475424472e-15 148 Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm YMR007W_d YMR007W hom 0.135081940879609 3.99636447669865e-15 149 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML121W GTR1 hom FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|GENE SILENCING|NUCLEUS 0.134764203510736 4.63663923801281e-15 150 Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB phosphate ion transport|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|chromatin silencing at telomere GDP binding|GTP binding fungal-type vacuole membrane|EGO complex|nucleus|late endosome membrane|cytoplasm YFR049W YMR31 hom FT TRANSLATION|MITOCHONDRION|RIBOSOME 0.134536853157113 5.15570892057039e-15 151 Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 cytoplasmic translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YLR172C DPH5 hom 0.134053500790772 6.63755141836319e-15 152 Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-diphthamide biosynthetic process from peptidyl-histidine diphthine synthase activity cytoplasm YKL061W_p BLI1 hom 0.133317800885957 9.4165032444192e-15 153 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome biological_process molecular_function endosome YOR275C RIM20 hom FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS|NUCLEUS 0.133190023562336 9.63146550404906e-15 154 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation proteolysis|response to pH|protein processing|invasive growth in response to glucose limitation|sporulation resulting in formation of a cellular spore molecular_function nucleus|cytoplasm YDR072C IPT1 hom FT LIPID METABOLISM 0.132997199741573 1.05274763173975e-14 155 Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin mannosyl diphosphorylinositol ceramide metabolic process|sphingolipid biosynthetic process transferase activity, transferring phosphorus-containing groups integral to membrane YLR085C ARP6 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.132375621334551 1.41375861023786e-14 156 Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin remodeling|histone exchange nucleosome binding Swr1 complex|cytoplasm YOR036W PEP12 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.131848038896969 1.79995438230867e-14 157 Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Golgi to vacuole transport|vacuole inheritance SNAP receptor activity endosome|Golgi apparatus YGL100W SEH1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE|NUCLEAR PORE 0.131645886354661 2.00935787901258e-14 158 Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 nucleocytoplasmic transport structural constituent of nuclear pore fungal-type vacuole membrane|integral to membrane|nuclear pore|Seh1-associated complex|nuclear pore outer ring YDR125C ECM18 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.131299688630807 2.29082557968387e-14 159 Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication fungal-type cell wall organization molecular_function mitochondrion YDR359C EAF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.131129543452747 2.47512512292938e-14 160 Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 DNA repair|cellular protein complex assembly|chromatin modification molecular_function histone acetyltransferase complex|NuA4 histone acetyltransferase complex YGR055W MUP1 hom FT PLASMA MEMBRANE 0.130481488405121 3.75212620623339e-14 161 High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake cysteine transport|sulfur amino acid transport|methionine transport L-methionine secondary active transmembrane transporter activity integral to membrane|integral to plasma membrane YPL237W SUI3 het FT TRANSLATION|RIBOSOME 0.130090789651866 3.96106863367758e-14 162 Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding translational initiation translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YDR395W SXM1 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.129927716610521 4.2630861597317e-14 163 Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 mRNA export from nucleus|nucleocytoplasmic transport protein transporter activity integral to membrane|nucleus|cytoplasm YLR200W YKE2 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.12942919789305 5.33372416723263e-14 164 Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding|tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YLR417W VPS36 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.128339206918713 8.67926017778317e-14 165 Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome negative regulation of transcription from RNA polymerase II promoter by glucose|protein retention in Golgi apparatus|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole ubiquitin binding ESCRT II complex YDR073W SNF11 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RSC COMPLEX 0.128073112718973 1.01029441728339e-13 166 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YML062C MFT1 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.127842420759286 1.16689652669307e-13 167 Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance mRNA export from nucleus|DNA recombination|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YGL083W SCY1 hom FT PROTEIN PHOSPHORYLATION 0.127726358942999 1.13916377075254e-13 168 Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase biological_process molecular_function clathrin-coated vesicle YLR143W DPH6 hom 0.127032256036163 1.54762626025506e-13 169 Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene biological_process molecular_function cytoplasm YDR524C-B_p YDR524C-B hom FT PLASMA MEMBRANE 0.126343507325168 2.09412938238694e-13 170 Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YHL009C YAP3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.125678455499688 2.79992144561345e-13 171 Basic leucine zipper (bZIP) transcription factor regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YLR408C_p BLS1 hom 0.124727586582123 4.26396759026116e-13 172 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene biological_process molecular_function endosome|BLOC-1 complex YNL246W VPS75 hom FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.124669039633353 4.33846457577202e-13 173 NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress double-strand break repair via nonhomologous end joining|nucleosome assembly|positive regulation of histone acetylation histone binding|acetyltransferase activator activity chromatin|nucleus YNL086W_p SNN1 hom 0.123783261827022 6.40305738023623e-13 174 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes biological_process molecular_function endosome|BLOC-1 complex YPR092W_d YPR092W hom 0.123779387769103 6.61866285891535e-13 175 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL035C CKA1 hom FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.123632608723014 6.77682472158289e-13 176 Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase activity|protein kinase activity protein kinase CK2 complex|UTP-C complex YGL198W YIP4 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.123540463417156 7.38894153625801e-13 177 Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process Rab GTPase binding integral to membrane|Golgi apparatus YBR231C SWC5 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.123423917799488 7.41023705651748e-13 178 Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress chromatin remodeling|histone exchange molecular_function Swr1 complex|nucleus YMR099C YMR099C hom FT CARBOHYDRATE METABOLISM|NUCLEUS 0.123407225540144 7.46333938244159e-13 179 Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS cellular carbohydrate metabolic process glucose-6-phosphate 1-epimerase activity nucleus|cytoplasm YNL147W LSM7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.123375056578155 7.56673224214863e-13 180 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasm YHR079C IRE1 hom FF|FT RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.123206491550848 8.13191597977065e-13 181 Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|inositol metabolic process|protein phosphorylation unfolded protein binding|protein serine/threonine kinase activity|endoribonuclease activity|protein kinase activity|protein homodimerization activity nucleus YDR392W SPT3 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.122911560302312 9.22205586746459e-13 182 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters conjugation with cellular fusion|histone acetylation|pseudohyphal growth|ascospore formation|chromatin modification|invasive growth in response to glucose limitation|transcription from RNA polymerase II promoter transcription cofactor activity SLIK (SAGA-like) complex|SAGA complex YLR119W SRN2 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.12255478047962 1.07336089116066e-12 183 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole protein binding endosome|ESCRT I complex YBR287W_p YBR287W hom FT ENDOPLASMIC RETICULUM 0.121847428088476 1.44834411699139e-12 184 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YIL069C RPS24B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.121703257303478 1.55097472461997e-12 185 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YOR085W OST3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.120676746042641 2.36913067877677e-12 186 Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins protein glycosylation|protein complex assembly|protein O-linked mannosylation|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YEL022W GEA2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.120512430657535 2.53759431180867e-12 187 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|secretory granule organization|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|extrinsic to membrane YOR073W SGO1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.120502861490627 2.54775892569511e-12 188 Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability positive regulation of maintenance of meiotic sister chromatid cohesion|meiotic sister chromatid segregation|meiotic sister chromatid separation|sister chromatid biorientation|maintenance of meiotic sister chromatid cohesion|mitotic cell cycle spindle assembly checkpoint|mitotic sister chromatid segregation molecular_function condensed nuclear chromosome kinetochore|nucleus|spindle pole body|condensed nuclear chromosome, centromeric region YNL204C SPS18 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.120240727390744 2.8422632123252e-12 189 Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation sporulation resulting in formation of a cellular spore molecular_function cellular_component YOR295W UAF30 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.119239845467942 4.30633982270436e-12 190 Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication transcription of nuclear large rRNA transcript from RNA polymerase I promoter|chromatin organization RNA polymerase I upstream control element sequence-specific DNA binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YGL188C-A_p YGL188C-A hom 0.118879349086832 4.99728689213314e-12 191 Putative protein of unknown function biological_process molecular_function cellular_component YGR060W ERG25 het FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.118507117640583 5.82452698815753e-12 192 C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol ergosterol biosynthetic process C-4 methylsterol oxidase activity plasma membrane|endoplasmic reticulum membrane YJL129C TRK1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.118292234886162 6.36167029132321e-12 193 Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication cellular potassium ion homeostasis potassium ion transmembrane transporter activity membrane raft|integral to membrane|plasma membrane YAL011W SWC3 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.118081327820353 6.93594964531304e-12 194 Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae endoplasmic reticulum organization|chromatin remodeling|histone exchange molecular_function mitochondrion|Swr1 complex|nucleus YHL029C_p OCA5 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.11801367469241 7.13069736432727e-12 195 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm YOR070C GYP1 hom FF|FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.11785823986188 7.76310937630362e-12 196 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion vesicle-mediated transport Rab GTPase activator activity mitochondrion|Golgi apparatus YNR044W AGA1 hom 0.117437404044187 9.02181593377334e-12 197 Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds agglutination involved in conjugation with cellular fusion cell adhesion molecule binding fungal-type cell wall YDL081C RPP1A hom FT TRANSLATION|RIBOSOME 0.117379530646336 9.2369055171348e-12 198 Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component cytoplasmic translation|translational elongation structural constituent of ribosome cytosolic large ribosomal subunit YMR161W HLJ1 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.116475839670342 1.33247948465308e-11 199 Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ ER-associated protein catabolic process ATPase activator activity endoplasmic reticulum|endoplasmic reticulum membrane YDR407C TRS120 het FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.115139096073585 2.27942420581576e-11 200 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic regulation of Rab GTPase activity|early endosome to Golgi transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity trans-Golgi network|TRAPP complex|early endosome YJL086C_d YJL086C het FF 0.114785201858519 2.69698524853006e-11 201 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Unknown Unknown Unknown YIL170W HXT12 hom 0.114513510932461 2.92436978873544e-11 202 Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family hexose transport|biological_process hexose transmembrane transporter activity|molecular_function integral to membrane YMR154C RIM13 hom FF|FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS 0.1142101460194 3.55093523579775e-11 203 Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB protein processing cysteine-type endopeptidase activity cellular_component YGR200C ELP2 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.113968947748785 3.62870855927673e-11 204 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin regulation of transcription from RNA polymerase II promoter|protein urmylation|tRNA wobble uridine modification molecular_function Elongator holoenzyme complex|nucleus|cytoplasm YLR268W SEC22 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.11391914272864 3.70085109798523e-11 205 R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion with endoplasmic reticulum|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|Golgi apparatus|endoplasmic reticulum|ER to Golgi transport vesicle membrane YDL070W BDF2 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS 0.113411804411175 4.64167430396368e-11 206 Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress negative regulation of heterochromatin assembly histone acetyl-lysine binding|core promoter binding|histone binding|TFIID-class transcription factor binding nucleus YLR055C SPT8 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.113332511335666 4.66364793945136e-11 207 Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters histone acetylation|negative regulation of transcription from RNA polymerase II promoter|chromatin modification|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|transcription cofactor activity nucleus|SAGA complex YGR057C LST7 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.113099994603914 5.10961952291726e-11 208 Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface intracellular protein transport|vesicle-mediated transport|protein transport|Golgi to plasma membrane transport protein transporter activity vesicle coat YPL002C SNF8 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.112997739057745 5.31870598026601e-11 209 Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression negative regulation of transcription from RNA polymerase II promoter by glucose|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole molecular_function ESCRT II complex YPL084W BRO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.112667043282068 6.05379965670787e-11 210 Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes protein deubiquitination|vacuolar transport|protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|response to nutrient|ubiquitin-dependent protein catabolic process ubiquitin-specific protease activity endosome|cytoplasm YDR495C VPS3 hom FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.112534912715835 6.37450460995702e-11 211 Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase vacuole inheritance|vacuolar acidification|protein targeting to vacuole molecular_function CORVET complex|cytoplasm YLR330W CHS5 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.112152258422746 7.39988392287148e-11 212 Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane ascospore wall assembly|conjugation with cellular fusion|cell wall chitin catabolic process|Golgi to plasma membrane transport|cellular bud site selection|regulation of transcription, DNA-dependent molecular_function mating projection tip|cytoplasm|exomer complex YDR193W_d YDR193W hom 0.112072480822359 8.09029615084816e-11 213 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL212W VAM7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.111920454740595 8.4714171929618e-11 214 Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress piecemeal microautophagy of nucleus|vacuole fusion, non-autophagic|vesicle fusion SNAP receptor activity|phosphatidylinositol-3-phosphate binding SNARE complex|fungal-type vacuole membrane YDR081C PDC2 het FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.111676676835436 8.90087164844991e-11 215 Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions positive regulation of thiamine biosynthetic process|Unknown|positive regulation of transcription from RNA polymerase II promoter transcription regulatory region sequence-specific DNA binding nucleus YMR261C TPS3 hom FT CARBOHYDRATE METABOLISM 0.111325160060268 1.01976765307571e-10 216 Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication trehalose biosynthetic process|response to stress enzyme regulator activity|alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity|trehalose-phosphatase activity alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) YBL051C PIN4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE 0.111006896995202 1.15298662505917e-10 217 Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage G2/M transition of mitotic cell cycle|DNA damage checkpoint molecular_function cytoplasm YGR122W YGR122W hom FF|FT TRANSCRIPTION FROM RNA POL II 0.11091064905614 1.19652152027209e-10 218 Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function cytoplasm YJR040W GEF1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.110537344113795 1.38108814292874e-10 219 Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism cellular copper ion homeostasis|cellular iron ion homeostasis voltage-gated chloride channel activity endosome|integral to membrane|endoplasmic reticulum|Golgi apparatus|plasma membrane|fungal-type vacuole|Golgi medial cisterna YBR175W SWD3 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.109814577107537 1.83212429292295e-10 220 Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 histone H3-K4 methylation|telomere maintenance|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YCR049C_d YCR049C hom 0.109438607386549 2.10084591869675e-10 221 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR202W ERG2 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.10938405355319 2.23935780897965e-10 222 C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis ergosterol biosynthetic process C-8 sterol isomerase activity endoplasmic reticulum YBL015W ACH1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.109319664351378 2.19790141990129e-10 223 Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth acetate metabolic process acetate CoA-transferase activity|acetyl-CoA hydrolase activity cytosol|mitochondrion YGL218W_d YGL218W hom 0.108055921548666 3.64900289550543e-10 224 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YNL115C_p YNL115C hom 0.107882094651778 3.77933727382549e-10 225 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene biological_process molecular_function integral to membrane|fungal-type vacuole YNL251C NRD1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.107779601330071 3.92719879072671e-10 226 RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites mRNA 3'-end processing|nuclear mRNA surveillance|termination of RNA polymerase II transcription, exosome-dependent|antisense RNA transcript catabolic process|tRNA 3'-end processing|snRNA 3'-end processing|snoRNA 3'-end processing|CUT catabolic process RNA binding|transcription regulatory region RNA binding|protein domain specific binding Nrd1 complex|nucleus YKL039W PTM1 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.107296701197337 4.70329966408052e-10 227 Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YKL207W EMC3 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.107167234003383 4.93562350503972e-10 228 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane YLR217W_d YLR217W hom 0.107084349998206 5.18123305442838e-10 229 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Unknown Unknown Unknown YBR291C CTP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.106885557498367 5.48041754925527e-10 230 Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family mitochondrial citrate transport|transmembrane transport tricarboxylate secondary active transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YDL047W SIT4 hom FT CELL CYCLE|RNA PROCESSING|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.106821484025856 5.61229956717879e-10 231 Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization intracellular protein kinase cascade|replicative cell aging|G1/S transition of mitotic cell cycle|actin cytoskeleton organization|DNA repair|cellular response to oxidative stress|fungal-type cell wall organization|TOR signaling cascade|dephosphorylation|tRNA wobble uridine modification protein serine/threonine phosphatase activity nucleus|cytoplasm YJL169W_d YJL169W hom 0.106468349871422 6.3966058859483e-10 232 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Unknown Unknown Unknown YOR198C BFR1 hom FT CELL CYCLE|ENDOMEMBRANE SYSTEM 0.106202539166475 7.05650211040678e-10 233 Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity meiosis|mRNA metabolic process|regulation of mitosis RNA binding ribonucleoprotein complex|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YFL031W HAC1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|NUCLEUS 0.105135404332072 1.04406294622532e-09 234 Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress endoplasmic reticulum unfolded protein response|positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response|negative regulation of transcription from RNA polymerase II promoter during meiosis|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YBR111W-A SUS1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE|HISTONE ACETYLTRANSFERASE 0.105030273793365 1.12258813039115e-09 235 Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP poly(A)+ mRNA export from nucleus|regulation of protein localization|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|histone deubiquitination|histone H3-K4 methylation|transcription elongation from RNA polymerase II promoter|histone H3-K79 methylation|positive regulation of transcription from RNA polymerase II promoter enzyme activator activity DUBm complex|nucleoplasm|transcription export complex 2|SAGA complex|nuclear pore YDR323C PEP7 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.10440555896695 1.36183420074017e-09 236 Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Golgi to vacuole transport|vacuole inheritance|vesicle fusion|vesicle docking involved in exocytosis|Golgi to endosome transport phosphatidylinositol-3-phosphate binding external side of endosome membrane|cytoplasm YBR154C RPB5 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.104288708603658 1.50276569397464e-09 237 RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YER171W RAD3 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.104109513138959 1.51603621353714e-09 238 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress regulation of transposition, RNA-mediated|phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair, DNA incision|regulation of mitotic recombination|transcription from RNA polymerase II promoter damaged DNA binding|ATP-dependent 5'-3' DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YLR407W_p YLR407W hom 0.103989020766775 1.5835552322598e-09 239 Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus biological_process molecular_function cellular_component YML007C-A_p YML007C-A hom FF|FT MITOCHONDRION 0.103705580500381 1.75413968917581e-09 240 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria biological_process molecular_function mitochondrion YLR451W LEU3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.103290293722899 2.04805044060092e-09 241 Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YLR324W PEX30 hom FF|FT PEROXISOME ORGANIZATION 0.102906932473836 2.3368062747019e-09 242 Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function integral to membrane|peroxisome|integral to peroxisomal membrane YLL014W EMC6 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.102819668502143 2.47755563555092e-09 243 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane|endoplasmic reticulum YEL044W IES6 hom FT NUCLEUS 0.102752685626109 2.50998369769144e-09 244 Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes biological_process molecular_function nucleus YDR490C PKH1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION 0.102404990826082 2.85647700200089e-09 245 Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p regulation of nuclear-transcribed mRNA poly(A) tail shortening|endocytosis|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cytosol|cell cortex|eisosome YIL107C PFK26 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.102382856996802 2.81740903891613e-09 246 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YDR526C_d YDR526C het FF 0.102020509266952 3.25668528919989e-09 247 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER119C-A_d YER119C-A hom FF 0.101741459872301 3.53763430857106e-09 248 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Unknown Unknown Unknown YDL167C NRP1 hom 0.101663815990118 3.63613352403926e-09 249 Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis biological_process molecular_function cytoplasmic stress granule|cytoplasm YJL148W RPA34 hom FT NUCLEUS 0.101514210664232 3.83350301367696e-09 250 RNA polymerase I subunit A34.5 transcription elongation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter molecular_function DNA-directed RNA polymerase I complex|nucleolus|nucleus YDL041W_d YDL041W hom FF 0.101480402810329 3.87952779110034e-09 251 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Unknown Unknown Unknown YJL168C SET2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.101415089599676 3.96997021114193e-09 252 Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p negative regulation of histone H3-K14 acetylation|DNA-dependent transcription, elongation|ascospore formation|regulation of transcription, DNA-dependent|histone deacetylation|regulation of DNA-dependent DNA replication initiation|histone methylation|negative regulation of antisense RNA transcription|Unknown|negative regulation of reciprocal meiotic recombination|positive regulation of histone acetylation histone methyltransferase activity|histone methyltransferase activity (H3-K36 specific) nucleus YJR083C ACF4 hom 0.101153746502055 4.35283377757655e-09 253 Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate biological_process molecular_function cellular_component YML021C UNG1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.101045568538444 4.52161695866102e-09 254 Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus DNA repair uracil DNA N-glycosylase activity mitochondrion|nucleus YDL226C GCS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|CYTOSKELETON 0.100990335584895 4.61023134902639e-09 255 ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p retrograde vesicle-mediated transport, Golgi to ER|Golgi to plasma membrane protein transport|actin filament reorganization involved in cell cycle|ER to Golgi vesicle-mediated transport actin binding|ARF GTPase activator activity|GTPase activator activity endosome|trans-Golgi network|endoplasmic reticulum-Golgi intermediate compartment|cytoskeleton YML107C PML39 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.100782332734765 4.95935717206064e-09 256 Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes maintenance of RNA location molecular_function ribosome|nuclear pore YPR041W TIF5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.100175785337235 6.13070622380386e-09 257 Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 mature ribosome assembly|negative regulation of translational initiation|regulation of translational initiation translation initiation factor binding|GDP-dissociation inhibitor activity|GTPase activator activity|translation initiation factor activity cytosolic small ribosomal subunit|multi-eIF complex YHL011C PRS3 hom FF|FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.10001861507857 6.47566017183114e-09 258 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process|regulation of cell size ribose phosphate diphosphokinase activity cytoplasm YLR021W IRC25 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0998847664149787 6.88997900318382e-09 259 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions proteasome assembly|chaperone-mediated protein complex assembly|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function intracellular YLR068W FYV7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0997018350372126 7.41727464835328e-09 260 Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YKR019C IRS4 hom FF|FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|MITOCHONDRION 0.0993596324342426 8.56499759531441e-09 261 EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication autophagy|fungal-type cell wall organization|chromatin silencing at rDNA|inositol lipid-mediated signaling|CVT pathway|cellular response to starvation molecular_function mitochondrion|pre-autophagosomal structure YKL092C BUD2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0989667918861369 9.32079516321183e-09 262 GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint bipolar cellular bud site selection|small GTPase mediated signal transduction|filamentous growth|cellular bud site selection|axial cellular bud site selection GTPase activator activity|Ras GTPase activator activity intracellular YMR070W MOT3 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0989499162044713 9.37513394595048e-09 263 Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion cellular hyperosmotic response|negative regulation of transcription from RNA polymerase II promoter|negative regulation of ergosterol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding nucleus YGR206W MVB12 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|VACUOLAR TRANSPORT 0.09884756554485 9.71135240206836e-09 264 ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin negative regulation of protein complex assembly|protein targeting to vacuole|endosome transport via multivesicular body sorting pathway ubiquitin binding cytosol|endosome|ESCRT I complex YJL026W RNR2 het FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|NUCLEUS 0.0986932500095854 1.02922185634858e-08 265 Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ribonucleoside-diphosphate reductase complex|nucleus|cytoplasm YKL051W SFK1 hom FT LIPID METABOLISM|SIGNALING|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0984107580818499 1.12827220074763e-08 266 Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane vacuole organization|inositol lipid-mediated signaling|actin cytoskeleton organization molecular_function integral to membrane|plasma membrane YOL159C YOL159C hom 0.098330830148003 1.15958374118067e-08 267 Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA biological_process molecular_function cellular_component YJL094C KHA1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|GOLGI APPARATUS 0.0978618473105429 1.40904931573895e-08 268 Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies monovalent inorganic cation transport potassium:hydrogen antiporter activity mitochondrion|integral to membrane|Golgi apparatus YPL243W SRP68 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0978051508419458 1.42938402699723e-08 269 Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding signal recognition particle, endoplasmic reticulum targeting YPR060C ARO7 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0972835081264423 1.6565935624415e-08 270 Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis aromatic amino acid family biosynthetic process chorismate mutase activity nucleus|cytoplasm YIL135C VHS2 hom FT CELL CYCLE 0.0971706882149397 1.72110818479771e-08 271 Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p G1/S transition of mitotic cell cycle molecular_function cytoplasm YNL011C_p YNL011C hom 0.0966484078450132 2.05294400059602e-08 272 Putative protein of unknown function; YNL011C is not an essential gene biological_process molecular_function cellular_component YGR163W GTR2 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|MEMBRANE ORGANIZATION|NUCLEUS|CHROMOSOME 0.0960953198498861 2.47189029560573e-08 273 Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD microautophagy|positive regulation of transcription from RNA polymerase II promoter GTP binding nuclear chromatin|fungal-type vacuole membrane|integral to membrane|EGO complex|nucleus|late endosome membrane|cytoplasm YBR189W RPS9B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.0959468945974533 2.59774752051597e-08 274 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication positive regulation of translational fidelity|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YDL013W SLX5 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.09587678063862 2.65934128346773e-08 275 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers protein sumoylation|telomere maintenance|response to DNA damage stimulus|protein ubiquitination SUMO binding|ubiquitin-protein ligase activity ubiquitin ligase complex|nucleus YOR030W DFG16 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0951409205234822 3.47801868205511e-08 276 Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p pseudohyphal growth|protein processing molecular_function integral to membrane YBL079W NUP170 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0946040384264461 4.11443736482081e-08 277 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication nucleocytoplasmic transport|chromosome segregation|protein targeting to nuclear inner membrane|mitotic cell cycle spindle assembly checkpoint|nuclear pore complex assembly structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YFL063W_d YFL063W hom 0.0945101411019249 4.26336419885847e-08 278 Dubious open reading frame, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR466W PKH3 hom FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION 0.0943640588852401 4.39142332372438e-08 279 Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant MAPK cascade involved in cell wall biogenesis|protein phosphorylation protein kinase activity cellular_component YPR070W MED1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.093778030979928 5.32229248770413e-08 280 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YDR443C SSN2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0937757498261213 5.35059179163406e-08 281 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II transcription coactivator activity|RNA polymerase II transcription factor recruiting transcription factor activity mediator complex YHR151C MTC6 hom 0.0936796200372681 5.49630404924142e-08 282 Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YMR264W CUE1 hom FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.093613227861487 5.6167993640923e-08 283 Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication ER-associated protein catabolic process|establishment of protein localization to endoplasmic reticulum membrane ubiquitin-protein ligase activator activity Doa10p ubiquitin ligase complex|mitochondrion|integral to endoplasmic reticulum membrane YNL293W MSB3 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0933064923219531 6.23559658289368e-08 284 Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress actin filament organization|exocytosis Rab GTPase activator activity incipient cellular bud site|polarisome|cellular bud tip YMR104C YPK2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0932263307719962 6.5461313518036e-08 285 Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication eisosome assembly|protein phosphorylation protein kinase activity nucleus|cytoplasm YKR035W-A DID2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.0931797382284511 6.49799648344271e-08 286 Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors late endosome to vacuole transport via multivesicular body sorting pathway|late endosome to vacuole transport|protein targeting to vacuole molecular_function late endosome|cytoplasm YHL014C YLF2 hom FT MITOCHONDRION 0.0927013178897542 7.55442700760107e-08 287 Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process GTP binding mitochondrion YDR110W FOB1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0926352615642358 7.71752849831021e-08 288 Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases extrachromosomal circular DNA accumulation involved in cell aging|negative regulation of DNA replication|rDNA condensation|chromatin silencing at rDNA|replicative cell aging|protein localization to nucleolar rDNA repeats|DNA recombination rDNA spacer replication fork barrier binding nucleolus|rDNA heterochromatin YPR139C LOA1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.0924776026558698 8.19305206626312e-08 289 Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA lipid particle organization|cellular triglyceride homeostasis|protein targeting to vacuole lysophosphatidic acid acyltransferase activity endoplasmic reticulum|lipid particle|cytoplasm YIL027C EMC5 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0924058955799512 8.34763605621165e-08 290 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YKL053W_d YKL053W hom 0.0922664455575687 8.73121179303213e-08 291 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Unknown Unknown Unknown YOR202W HIS3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0921720821771804 8.96081827952768e-08 292 Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p histidine biosynthetic process imidazoleglycerol-phosphate dehydratase activity cellular_component YAL042W ERV46 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.091594733338818 1.07837847952925e-07 293 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YJR073C OPI3 hom FF|FT LIPID METABOLISM|MITOCHONDRION 0.0913362001078174 1.19681701472188e-07 294 Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis phosphatidylcholine biosynthetic process phosphatidyl-N-dimethylethanolamine N-methyltransferase activity|phosphatidyl-N-methylethanolamine N-methyltransferase activity mitochondrion|integral to membrane YGR182C_d YGR182C hom 0.0912236306544181 1.23512788237178e-07 295 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W Unknown Unknown Unknown YOR164C GET4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0910046708796129 1.30156708190199e-07 296 Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 protein insertion into ER membrane|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YGR127W_p YGR127W hom 0.0905891442039747 1.48489055921284e-07 297 Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component YGR135W PRE9 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0905372828136804 1.50945247153063e-07 298 Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform proteasomal ubiquitin-dependent protein catabolic process|proteasome core complex assembly|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YMR039C SUB1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0904707958407097 1.54151617293377e-07 299 Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress termination of RNA polymerase II transcription|hyperosmotic response|RNA polymerase III transcriptional preinitiation complex assembly|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase III promoter transcription coactivator activity nucleus YML001W YPT7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|MITOCHONDRION 0.0903957525460352 1.61233898529726e-07 300 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole inheritance|Golgi to vacuole transport|vesicle-mediated transport|endocytosis GTPase activity mitochondrial outer membrane|fungal-type vacuole YGR237C_p YGR237C hom 0.0901106371512004 1.75635138221328e-07 301 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YCL010C SGF29 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.089797250220372 1.90574610016882e-07 302 Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation histone H3-K14 acetylation|cellular protein complex localization|histone H3-K18 acetylation|cellular protein localization|histone H3-K9 acetylation|positive regulation of transcription from RNA polymerase II promoter methylated histone residue binding|RNA polymerase II transcription factor recruiting transcription factor activity Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YER031C YPT31 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0896598749338749 1.99795587564476e-07 303 Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication early endosome to Golgi transport|vesicle-mediated transport|exocytosis GTPase activity mitochondrial outer membrane|endosome|Golgi apparatus YOL016C CMK2 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.0896491880855619 1.99630387805968e-07 304 Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YMR067C UBX4 hom FT PROTEOLYSIS|NUCLEUS 0.0895087617588445 2.08602351865277e-07 305 UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress ER-associated protein catabolic process|sporulation resulting in formation of a cellular spore protein complex binding nucleus|cytoplasm|extrinsic to membrane YAL059W ECM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|CELL WALL ORG/BIOGENESIS|NUCLEUS 0.0895086250183603 2.0861127529788e-07 306 Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm fungal-type cell wall organization|ribosomal large subunit export from nucleus molecular_function nucleolus|nucleus|preribosome, large subunit precursor YBR260C RGD1 hom FT SIGNALING|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0894601125848557 2.11800488068059e-07 307 GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization response to acid|osmosensory signaling pathway Rho GTPase activator activity|phosphatidylinositol-4,5-bisphosphate binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding|phosphatidylinositol-5-phosphate binding cellular bud|mating projection tip|actin cortical patch YDR417C_d YDR417C hom 0.0894575857898786 2.11967883819195e-07 308 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Unknown Unknown Unknown YFL015C_d YFL015C hom 0.089329587704834 2.20616781052174e-07 309 Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Unknown Unknown Unknown YNL032W SIW14 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0892357405193262 2.2717363476731e-07 310 Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm actin filament organization|endocytosis|protein dephosphorylation|response to stress protein tyrosine phosphatase activity cytoplasm YNL310C ZIM17 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0890494341500822 2.40752525326418e-07 311 Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix protein folding|protein stabilization|protein import into mitochondrial matrix|response to unfolded protein|mitochondrion organization chaperone binding mitochondrion|mitochondrial matrix YOR004W UTP23 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0887366105113098 2.65333811096163e-07 312 Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|mitochondrion|nucleolus YCR094W CDC50 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS 0.0886057438158139 2.82016702520725e-07 313 Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication intracellular protein transport|phospholipid translocation|endocytosis|actin cortical patch localization phospholipid-translocating ATPase activity trans-Golgi network|late endosome membrane YDR141C DOP1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0884858407883956 2.86772064595943e-07 314 Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies endoplasmic reticulum organization|cell morphogenesis|Golgi to endosome transport molecular_function trans-Golgi network|endosome|mitochondrion|cytoplasm YDL052C SLC1 hom FT LIPID METABOLISM 0.0878161634218007 3.52537549325314e-07 315 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes glycerophospholipid biosynthetic process 1-acylglycerol-3-phosphate O-acyltransferase activity lipid particle YMR273C ZDS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL WALL ORG/BIOGENESIS|RNA LOCALIZATION|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH|NUCLEAR PORE 0.0874501121857125 3.94407669967084e-07 316 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication mRNA export from nucleus|positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|chromatin silencing|establishment of cell polarity|regulation of protein localization|cell aging protein phosphatase type 2A regulator activity incipient cellular bud site|nuclear pore|cellular bud neck|cellular bud tip|cytoplasm YCR052W RSC6 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.08735490631988 4.14200079923685e-07 317 Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p nucleosome positioning|ATP-dependent chromatin remodeling|G2/M transition of mitotic cell cycle|G1 phase of mitotic cell cycle|nucleosome disassembly|positive regulation of transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YDR360W_d OPI7 hom 0.0872806223704057 4.15381894564775e-07 318 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Unknown Unknown Unknown YLR381W CTF3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.087200979567753 4.25605255065281e-07 319 Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 chromosome segregation|DNA replication initiation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YML019W OST6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0870683132047589 4.43175971564406e-07 320 Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p protein N-linked glycosylation via asparagine|protein complex assembly dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YAL021C CCR4 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS|RNA POL II, HOLOENZYME 0.0870322414804064 4.48073082254862e-07 321 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|DNA replication checkpoint|nuclear-transcribed mRNA poly(A) tail shortening|traversing start control point of mitotic cell cycle|replication fork protection|transcription elongation from RNA polymerase II promoter|DNA replication 3'-5'-exoribonuclease activity CCR4-NOT core complex|cytoplasmic mRNA processing body|Cdc73/Paf1 complex|cytoplasm YCR085W_d YCR085W hom 0.0868684119264238 4.72832005783753e-07 322 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR404W FLD1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0866198741532987 5.07897266288932e-07 323 Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy lipid particle organization molecular_function integral to membrane|endoplasmic reticulum|cortical endoplasmic reticulum YJL032W_d YJL032W het 0.0865110514236501 5.24926090838689e-07 324 Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Unknown Unknown Unknown YNL203C_d YNL203C hom 0.0862382698501919 5.70064847846877e-07 325 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL036C YBL036C hom 0.0854341693659373 7.25952551906372e-07 326 Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process pyridoxal phosphate binding cellular_component YLR308W CDA2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0854176061515492 7.2955990515967e-07 327 Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall ascospore wall assembly chitin deacetylase activity chitosan layer of spore wall YCR064C_d YCR064C hom FF 0.0853183626698369 7.54393921220677e-07 328 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Unknown Unknown Unknown YNL307C MCK1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION 0.0852516935833889 7.6666006466695e-07 329 Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family meiosis|double-strand break repair via nonhomologous end joining|ascospore formation|protein phosphorylation|response to stress|mitotic sister chromatid segregation protein serine/threonine/tyrosine kinase activity|protein serine/threonine kinase activity|protein kinase activity cellular_component YER025W GCD11 het FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0851857207786792 7.81911612295006e-07 330 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met positive regulation of translational fidelity GTP binding|tRNA binding|translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YDR479C PEX29 hom FT PEROXISOME ORGANIZATION 0.085172668867026 7.84963363911671e-07 331 Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress peroxisome organization molecular_function integral to membrane|peroxisomal membrane YLL040C VPS13 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|MITOCHONDRION|GOLGI APPARATUS 0.0850445665196915 8.15530820186078e-07 332 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively protein retention in Golgi apparatus|late endosome to vacuole transport|protein targeting to vacuole|ascospore-type prospore membrane assembly molecular_function endosome|mitochondrion|prospore membrane|extrinsic to membrane YGL095C VPS45 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0849899086240102 8.38331565335826e-07 333 Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment vacuole inheritance|CVT pathway|positive regulation of SNARE complex assembly|Golgi vesicle fusion to target membrane|Golgi to vacuole transport|vacuole organization|vacuolar acidification|protein targeting to vacuole|Golgi to endosome transport unfolded protein binding|SNARE binding cytosol|SNARE complex|Golgi membrane|extrinsic to membrane YLR315W NKP2 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0849317860580746 8.82301693532755e-07 334 Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 biological_process molecular_function spindle pole body|kinetochore YNL124W NAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0842420075939678 1.03477416901173e-06 335 RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p box H/ACA snoRNP assembly RNA binding nucleoplasm|nucleus YAL020C ATS1 hom FT RNA PROCESSING|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0836158191548152 1.24418090149781e-06 336 Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle microtubule-based process|budding cell bud growth|microtubule cytoskeleton organization|tRNA wobble uridine modification molecular_function cytoplasm YIL057C RGI2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.083354020375477 1.34331882182969e-06 337 Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose energy reserve metabolic process molecular_function cytoplasm YHR205W SCH9 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|PROTEIN PHOSPHORYLATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0832391324175509 1.38918308170105e-06 338 AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan protein phosphorylation|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|regulation of protein localization|positive regulation of transcription from RNA polymerase III promoter|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase I promoter|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|regulation of cell size|regulation of response to osmotic stress protein serine/threonine kinase activity chromatin|fungal-type vacuole membrane|nucleus|cytoplasm YLR271W_p YLR271W hom FT NUCLEUS 0.0831875593429893 1.53238810093502e-06 339 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YGL116W CDC20 het FF|FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0829674028578258 1.50372765182448e-06 340 Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress spindle checkpoint|activation of mitotic anaphase-promoting complex activity|meiotic anaphase I|positive regulation of protein catabolic process|activation of anaphase-promoting complex activity involved in meiotic cell cycle|positive regulation of mitotic metaphase/anaphase transition|cyclin catabolic process anaphase-promoting complex binding|ubiquitin-protein ligase activator activity mitotic checkpoint complex|anaphase-promoting complex YER064C VHR2 hom FT NUCLEUS 0.0828643811954382 1.54948487293104e-06 341 Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress regulation of transcription, DNA-dependent molecular_function nucleus YKL131W_d YKL131W hom 0.0824945621019938 1.72501734360604e-06 342 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL037C EGD1 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.082251235272937 1.87048390313606e-06 343 Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b 'de novo' cotranslational protein folding unfolded protein binding nascent polypeptide-associated complex YMR134W ERG29 het FT LIPID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0821270469310889 1.95237566397586e-06 344 Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes cellular iron ion homeostasis|mitochondrion organization molecular_function nuclear envelope|endoplasmic reticulum|cytoplasm YMR164C MSS11 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0820542386443854 1.95897577721654e-06 345 Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YDL019C OSH2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.081837560748842 2.08500842162294e-06 346 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability endocytosis|maintenance of cell polarity|sterol transport|exocytosis lipid binding|oxysterol binding|sterol transporter activity|1-phosphatidylinositol binding endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum|cellular bud neck YGL231C EMC4 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0816362369601929 2.20905958094476e-06 347 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YHR187W IKI1 hom FT RNA PROCESSING|NUCLEUS 0.0815379590799586 2.27216007533553e-06 348 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin tRNA modification tRNA binding|ATPase activity Elongator holoenzyme complex|nucleus|cytoplasm YJR098C_p YJR098C hom FT LIPID METABOLISM|MITOCHONDRION 0.0812450833657353 2.47062988163143e-06 349 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YMR022W UBC7 hom FT CHROMATIN ORGANIZATION|CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0811283294678532 2.55429898494397e-06 350 Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly fungal-type cell wall organization|chromatin assembly or disassembly|ER-associated protein catabolic process ubiquitin-protein ligase activity endoplasmic reticulum membrane YCL052C PBN1 het FT LIPID METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0811128625722335 2.565584842601e-06 351 Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X GPI anchor biosynthetic process|ER-associated protein catabolic process|protein processing mannosyltransferase activity endoplasmic reticulum YIL149C MLP2 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CYTOSKELETON 0.0809560629918918 2.68273207041812e-06 352 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length poly(A)+ mRNA export from nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|regulation of transcription, DNA-dependent|telomere tethering at nuclear periphery|spindle pole body organization|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery ribonucleoprotein complex binding nucleoplasm|nuclear envelope|mitochondrion|ribonucleoprotein complex|integral to membrane|spindle pole body YGR144W THI4 hom FT MITOCHONDRION ORGANIZATION|NUCLEUS 0.0807802394065465 2.82019414445928e-06 353 Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents thiamine biosynthetic process|mitochondrial genome maintenance|thiazole biosynthetic process ferrous iron binding cytosol YNL219C ALG9 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0807554870105924 2.84008026533253e-06 354 Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation mannosyltransferase activity integral to membrane|endoplasmic reticulum YBL043W ECM13 hom FT CELL WALL ORG/BIOGENESIS 0.0807488802453676 2.88447568640363e-06 355 Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication fungal-type cell wall organization molecular_function cellular_component YGR227W DIE2 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0803048059276666 3.22655969243543e-06 356 Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 dolichol-linked oligosaccharide biosynthetic process|protein N-linked glycosylation dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YPL213W LEA1 hom FT RNA PROCESSING|NUCLEUS 0.0800351367693134 3.48141118324567e-06 357 Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein mRNA splicing, via spliceosome molecular_function U2 snRNP|U2-type prespliceosome|cytoplasm YOL013C HRD1 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0800152990287542 3.50090197126101e-06 358 Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon fungal-type cell wall organization|ER-associated protein catabolic process ubiquitin-protein ligase activity Hrd1p ubiquitin ligase ERAD-M complex|integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YBR036C CSG2 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0799253852569463 3.59056205175826e-06 359 Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress cellular calcium ion homeostasis|glycosphingolipid biosynthetic process enzyme regulator activity integral to endoplasmic reticulum membrane|integral to membrane YGR081C SLX9 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0794517011395887 4.10043219095516e-06 360 Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YOR274W MOD5 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0792462901028389 4.34247280175181e-06 361 Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis tRNA modification tRNA dimethylallyltransferase activity cytosol|mitochondrion|nucleolus|nucleus YBR284W_p YBR284W hom FT NUCLEOTIDE METABOLISM 0.0792461491923025 4.34264344321661e-06 362 Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication biological_process AMP deaminase activity|molecular_function cellular_component YCL021W-A_p YCL021W-A hom 0.079119960127916 4.49806304717437e-06 363 Putative protein of unknown function biological_process molecular_function cellular_component YPL057C SUR1 hom FT LIPID METABOLISM 0.078948318959366 4.71803333960578e-06 364 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication glycosphingolipid biosynthetic process|mannosyl-inositol phosphorylceramide metabolic process|sphingolipid biosynthetic process mannosyltransferase activity|transferase activity, transferring glycosyl groups integral to membrane|intracellular YBR300C_d YBR300C hom 0.0788799472330312 4.80849400020637e-06 365 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Unknown Unknown Unknown YDL160C DHH1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|RNA LOCALIZATION 0.0787551767847481 4.99406736697194e-06 366 Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|cell morphogenesis involved in conjugation with cellular fusion|stress granule assembly|cytoplasmic mRNA processing body assembly protein binding|RNA helicase activity cytoplasmic mRNA processing body|cytoplasmic stress granule|cytoplasm YMR135C GID8 hom FT KETONE METABOLISM|CELL CYCLE|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.0786727848709877 5.09283398689451e-06 367 Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|traversing start control point of mitotic cell cycle molecular_function nucleus|GID complex|cytoplasm YEL005C VAB2 hom 0.0786641459427752 5.10503417169888e-06 368 Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter biological_process molecular_function cytoplasm YGL244W RTF1 hom FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|GENE SILENCING|NUCLEUS|CHROMOSOME|RNA POL II, HOLOENZYME 0.0783712135821949 5.5356803727267e-06 369 Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay mRNA 3'-end processing|snoRNA transcription from an RNA polymerase II promoter|regulation of chromatin silencing at telomere|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|regulation of histone H3-K4 methylation|regulation of transcription-coupled nucleotide-excision repair|negative regulation of transcription from RNA polymerase II promoter|recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex|global genome nucleotide-excision repair|snoRNA 3'-end processing|Unknown|transcription elongation from RNA polymerase II promoter RNA binding|RNA polymerase II transcription factor binding transcription factor activity nucleus|transcriptionally active chromatin|Cdc73/Paf1 complex YKL093W MBR1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0783556381918479 5.55952533142385e-06 370 Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants aerobic respiration molecular_function cellular_component YNL288W CAF40 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0782389951315629 5.7412486760502e-06 371 Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter molecular_function CCR4-NOT core complex YKL002W DID4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0781533929276664 5.93492913167126e-06 372 Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis protein retention in Golgi apparatus|intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YOR006C TSR3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0781522332637222 5.88009009029953e-06 373 Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress maturation of SSU-rRNA molecular_function nucleus|cytoplasm YGR169C PUS6 hom FT RNA PROCESSING|MITOCHONDRION 0.0776203867595011 6.80399170093965e-06 374 tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|cytoplasm YDL007W RPT2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0775396156786883 6.95590980173804e-06 375 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle positive regulation of protein catabolic process|proteasome regulatory particle assembly|peptide catabolic process|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex|nucleus YCL051W LRE1 hom FT CELL WALL ORG/BIOGENESIS 0.0774235391055159 7.17992227012227e-06 376 Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication fungal-type cell wall organization molecular_function cellular_component YBR017C KAP104 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SITE OF POLARIZED GROWTH 0.0773800718737995 7.26556990175171e-06 377 Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis protein import into nucleus|cell cycle|mRNA-binding (hnRNP) protein import into nucleus|transcription factor import into nucleus nuclear localization sequence binding cytosol|integral to membrane|cellular bud neck|cellular bud tip YJL121C RPE1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.0773365739479126 7.35225514601973e-06 378 D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress pentose-phosphate shunt ribulose-phosphate 3-epimerase activity cytosol YNL239W LAP3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS|MITOCHONDRION 0.0772477407419005 7.53236815463371e-06 379 Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH response to antibiotic|negative regulation of transcription from RNA polymerase II promoter|homocysteine catabolic process double-stranded DNA binding|cysteine-type peptidase activity|mRNA binding|single-stranded DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding mitochondrion|cytoplasm YJL165C HAL5 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.077035854857521 8.23148368097999e-06 380 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication cellular cation homeostasis|protein phosphorylation|cellular protein localization|protein dephosphorylation protein kinase activity cellular_component YPL176C TRE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.0769470202305959 8.17391489512479e-06 381 Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function fungal-type vacuole YCR082W AHC2 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0768378731959896 8.41942680333398e-06 382 Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex|nucleus|cytoplasm YBL061C SKT5 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0767716080056656 8.5719018474956e-06 383 Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication fungal-type cell wall chitin biosynthetic process|response to osmotic stress|cytokinesis enzyme activator activity incipient cellular bud site|cellular bud neck septin ring|cellular bud neck YGR068C ART5 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0764716264830054 9.29576883988306e-06 384 Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane ubiquitin-dependent endocytosis ubiquitin protein ligase binding cellular_component YML083C YML083C hom 0.0762858297057573 9.77299373123748e-06 385 Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component YBR281C DUG2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS|NUCLEUS 0.0759876480945084 1.05881177327744e-05 386 Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process peptidase activity|gamma-glutamyltransferase activity|omega peptidase activity nucleus|cytoplasm YBR048W RPS11B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.075794262623313 1.11510285952018e-05 387 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YNR064C YNR064C hom 0.0757535091302707 1.12732444026551e-05 388 Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides response to toxin epoxide hydrolase activity cellular_component YFL049W SWP82 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0757074293911204 1.14473862904792e-05 389 Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YDR144C MKC7 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|PLASMA MEMBRANE 0.075587922809132 1.17830959907889e-05 390 GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p proteolysis|fungal-type cell wall organization aspartic-type endopeptidase activity fungal-type cell wall YPR111W DBF20 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0751360725258518 1.32887018212745e-05 391 Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs cytokinesis after mitosis|protein phosphorylation|barrier septum assembly involved in cell cycle cytokinesis|regulation of mRNA catabolic process|exit from mitosis protein serine/threonine kinase activity spindle pole body|cellular bud neck|cytoplasm YLR402W_d YLR402W hom 0.0748577608225554 1.43900888203322e-05 392 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR064C YNG1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0747985114579173 1.47467172090726e-05 393 Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 histone acetylation|chromatin modification histone acetyltransferase activity|methylated histone residue binding NuA3 histone acetyltransferase complex YDR485C VPS72 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0747918468140139 1.45570109065301e-05 394 Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting chromatin remodeling|histone exchange histone binding Swr1 complex|nucleus|cytoplasm YDR290W_d YDR290W hom 0.0747770926903729 1.46138730091663e-05 395 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Unknown Unknown Unknown YNL196C_p SLZ1 hom 0.0746635265930203 1.51029057224675e-05 396 Sporulation-specific protein with a leucine zipper motif biological_process molecular_function cellular_component YNL227C JJJ1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0744805894534697 1.58024815852502e-05 397 Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|endocytosis|regulation of cell size|rRNA processing ATPase activator activity cytosol|mitochondrion|cytosolic large ribosomal subunit|nucleolus|cytoplasm YBR197C YBR197C hom FT NUCLEUS 0.0742466667280966 1.68046529954071e-05 398 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YDL191W RPL35A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0742105759226956 1.6964564115152e-05 399 Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YHL033C RPL8A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0741825055921703 1.70899391237856e-05 400 Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YCR035C RRP43 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0741779976043343 1.71101559393719e-05 401 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nucleoplasm|nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex)|cytoplasm YJR063W RPA12 hom FF|FT NUCLEUS 0.073884846801319 1.8474872139665e-05 402 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex termination of RNA polymerase I transcription|transcription of nuclear large rRNA transcript from RNA polymerase I promoter molecular_function DNA-directed RNA polymerase I complex YLR367W RPS22B hom FT TRANSLATION|RIBOSOME 0.0738705888212177 1.85438233694045e-05 403 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YJL130C URA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0736715484636112 1.95323328584432e-05 404 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP glutamine metabolic process|'de novo' pyrimidine nucleobase biosynthetic process|negative regulation of pyrimidine nucleobase metabolic process aspartate carbamoyltransferase activity|carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity mitochondrion|integral to membrane|cytoplasm YGL034C_d YGL034C hom 0.0736426716372331 1.96798467453234e-05 405 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL145W RPT1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0732821466666269 2.16131413112034e-05 406 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of protein catabolic process|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YIL154C IMP2' hom FF|FT RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS 0.0732794602888568 2.22486628777581e-05 407 Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat DNA repair|cellular carbohydrate metabolic process transcription coactivator activity cytoplasm YEL054C RPL12A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.073153834281554 2.23438184909613e-05 408 Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YLR267W_p BOP2 hom 0.0726155115943202 2.56733668223379e-05 409 Protein of unknown function biological_process molecular_function cellular_component YDL006W PTC1 hom FF|FT RNA PROCESSING|SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0725411832158957 2.69794005837168e-05 410 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation mitochondrion inheritance|inactivation of MAPK activity involved in osmosensory signaling pathway|pheromone-dependent signal transduction involved in conjugation with cellular fusion|tRNA splicing, via endonucleolytic cleavage and ligation|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YEL007W MIT1 hom FT NUCLEUS 0.0724992313738597 2.64520608632443e-05 411 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus|cytoplasm YLR205C HMX1 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.072336402973591 2.78095082607428e-05 412 ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants response to oxidative stress|cellular iron ion homeostasis|heme catabolic process heme oxygenase (decyclizing) activity endoplasmic reticulum|membrane|nuclear outer membrane YJR122W IBA57 hom FF|FT COFACTOR METABOLISM|MITOCHONDRION 0.0723279145482367 2.83351196737652e-05 413 Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system iron-sulfur cluster assembly molecular_function mitochondrion|mitochondrial matrix YOL081W IRA2 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0722636600802588 2.81764390535042e-05 414 GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication negative regulation of cAMP biosynthetic process|negative regulation of Ras protein signal transduction|positive regulation of Ras GTPase activity|response to stress Ras GTPase activator activity mitochondrion|integral to membrane|cytoplasm YDR173C ARG82 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0722551329856457 2.81603632440784e-05 415 Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes protein stabilization|negative regulation of transcription from RNA polymerase II promoter|phosphatidylinositol phosphorylation|regulation of arginine metabolic process|inositol phosphate biosynthetic process|positive regulation of transcription from RNA polymerase II promoter inositol-1,4,5-trisphosphate 3-kinase activity|inositol-1,4,5-trisphosphate 6-kinase activity|phosphatidylinositol 3-kinase activity|protein binding, bridging|inositol tetrakisphosphate 6-kinase activity|inositol tetrakisphosphate 3-kinase activity|inositol-1,3,4,5,6-pentakisphosphate kinase activity nucleus YKL175W ZRT3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.072107196182354 2.92461702506069e-05 416 Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency cellular zinc ion homeostasis|zinc ion transport zinc ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YKR007W MEH1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0720823066627313 2.98387860323514e-05 417 Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification microautophagy|vacuolar acidification molecular_function fungal-type vacuole membrane|EGO complex|late endosome membrane YNL043C_d YNL043C hom 0.0720181062897654 2.99191276494859e-05 418 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Unknown Unknown Unknown YGR123C PPT1 hom FT NUCLEUS 0.0719320737182496 3.05829336425456e-05 419 Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YDL138W RGT2 hom FT SIGNALING|PLASMA MEMBRANE 0.071902533029855 3.08140713120659e-05 420 Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication glucose transport|signal transduction|detection of glucose glucose transmembrane transporter activity|receptor activity|glucose binding integral to membrane|plasma membrane YBL026W LSM2 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0718409489022411 3.13012753840253e-05 421 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YPL125W KAP120 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0716809988621058 3.26010801190965e-05 422 Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p protein import into nucleus|transcription factor import into nucleus protein transporter activity|structural constituent of nuclear pore integral to membrane|nucleus|nuclear pore|cytoplasm YCR026C NPP1 hom FT NUCLEOTIDE METABOLISM|RESPONSE TO NUTRIENT LEVELS 0.071656895669827 3.28013391040824e-05 423 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication cellular response to phosphate starvation|phosphate-containing compound metabolic process|nucleoside triphosphate metabolic process nucleotide diphosphatase activity|nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|phosphodiesterase I activity cellular_component YDR210W_p YDR210W hom FT PLASMA MEMBRANE 0.0714360163101353 3.46916943474529e-05 424 Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YCR087W_d YCR087W hom 0.0711301783927259 3.74804998693685e-05 425 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Unknown Unknown Unknown YKL185W ASH1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0710138098755815 3.85964621297784e-05 426 Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|core promoter proximal region sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|cellular bud|Rpd3L complex YDR067C_p OCA6 hom 0.0709583615138263 3.91392280674849e-05 427 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT biological_process molecular_function cytoplasm YNL109W_d YNL109W hom 0.0709134184464639 3.95844662842083e-05 428 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Unknown Unknown Unknown YMR061W RNA14 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0707960512194377 4.07699577477956e-05 429 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation RNA binding|protein heterodimerization activity mRNA cleavage factor complex|mitochondrion|nucleus YJL097W PHS1 het FT KETONE METABOLISM|LIPID METABOLISM|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0707179394565242 4.15775216182851e-05 430 Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking vacuolar transport|sphingolipid biosynthetic process enoyl-CoA hydratase activity fungal-type vacuole membrane|integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|fungal-type vacuole|nuclear outer membrane-endoplasmic reticulum membrane network YBR083W TEC1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0705705330399521 4.31429931651044e-05 431 Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member pseudohyphal growth|chronological cell aging|positive regulation of transposition, RNA-mediated|invasive growth in response to glucose limitation|positive regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YGL008C PMA1 het FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0705131877555012 4.37669761756949e-05 432 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] proton transport|regulation of pH|transmembrane transport hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|membrane raft|plasma membrane YGR289C MAL11 hom FT CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0704681612139191 4.47299854821715e-05 433 Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization trehalose transport|maltose transport|disaccharide catabolic process trehalose transmembrane transporter activity|alpha-glucoside:hydrogen symporter activity|maltose:hydrogen symporter activity integral to membrane|plasma membrane YNL248C RPA49 hom FT NUCLEUS 0.0704498430614569 4.44661829715321e-05 434 RNA polymerase I subunit A49 transcription elongation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YML105C SEC65 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS 0.0703835300813246 4.5805241811234e-05 435 Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER 7S RNA binding signal recognition particle, endoplasmic reticulum targeting YMR214W SCJ1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOPLASMIC RETICULUM 0.0702975456392092 4.61908520720563e-05 436 One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins ER-associated protein catabolic process|protein folding in endoplasmic reticulum|response to unfolded protein chaperone binding endoplasmic reticulum lumen YER048C CAJ1 hom FT NUCLEUS 0.070220414733202 4.70883129204039e-05 437 Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly protein folding chaperone binding nucleus YFR039C_p YFR039C hom 0.0697518235187968 5.29059826009104e-05 438 Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component YBR267W REI1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|CELL DIVISION|RIBOSOME 0.0697472017816567 5.29666164348193e-05 439 Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network ribosomal large subunit biogenesis|nucleocytoplasmic transport|mitotic cell cycle|budding cell bud growth sequence-specific DNA binding cytosolic large ribosomal subunit|preribosome, large subunit precursor|cytoplasm YML067C ERV41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0696682220325424 5.40130010899852e-05 440 Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YLR363C NMD4 hom 0.0695957576080393 5.49902607736226e-05 441 Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process, nonsense-mediated decay molecular_function cytoplasm YOR319W HSH49 het FF|FT RNA PROCESSING|NUCLEUS 0.0694821959681809 5.65555097213035e-05 442 U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YDL037C BSC1 hom 0.0693617635098885 5.82615032300288e-05 443 Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function cellular_component YIL097W FYV10 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.0690833620942466 6.30273685340471e-05 444 Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|anti-apoptosis ubiquitin-protein ligase activity nucleus|GID complex|cytoplasm YOL133W HRT1 het FT CELL CYCLE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0689764317558832 6.40531469121014e-05 445 RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity SCF ubiquitin ligase complex|nucleus|cytoplasm YLR117C CLF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|NUCLEUS|CHROMOSOME 0.0689615856641423 6.42868367141818e-05 446 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins cis assembly of pre-catalytic spliceosome|DNA replication initiation DNA replication origin binding|chromatin binding|first spliceosomal transesterification activity|second spliceosomal transesterification activity chromatin|U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YEL003W GIM4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0689588124959675 6.58051679623669e-05 447 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YML070W DAK1 hom FT CARBOHYDRATE METABOLISM 0.0688508630528862 6.60553166523629e-05 448 Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation response to stress|glycerol catabolic process glycerone kinase activity cytoplasm YPR044C_d OPI11 hom 0.0686303063363644 7.02399496507983e-05 449 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Unknown Unknown Unknown YDL077C VAM6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION 0.0679082540857001 8.30890715948632e-05 450 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle-mediated transport|vacuole organization|vacuole fusion, non-autophagic Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding fungal-type vacuole membrane|HOPS complex|fungal-type vacuole YLL027W ISA1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0679041872219217 8.35785633519828e-05 451 Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins biotin biosynthetic process|iron-sulfur cluster assembly iron-sulfur cluster binding|iron ion binding mitochondrial matrix YLR024C UBR2 hom 0.067842055002553 8.44295258960217e-05 452 Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YLR350W ORM2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0673994040647146 9.39315181522506e-05 453 Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress cellular sphingolipid homeostasis|negative regulation of sphingolipid biosynthetic process|response to unfolded protein molecular_function integral to membrane|endoplasmic reticulum|SPOTS complex YPL227C ALG5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0672788743072194 9.66886161031242e-05 454 UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein N-linked glycosylation dolichyl-phosphate beta-glucosyltransferase activity endoplasmic reticulum membrane YIL105C SLM1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.0669842592870575 0.000103752861572912 455 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication eisosome assembly|TOR signaling cascade|regulation of cell growth|actin filament bundle assembly|endosomal transport|establishment or maintenance of actin cytoskeleton polarity|actin cytoskeleton organization phosphatidylinositol-4,5-bisphosphate binding|phosphatidylinositol binding|sphingolipid binding eisosome|mitochondrion|membrane raft|plasma membrane|cytoplasm YPR033C HTS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0665250266246672 0.000116288316720784 456 Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome histidyl-tRNA aminoacylation|mitochondrial translation aminoacyl-tRNA ligase activity mitochondrion|cytoplasm YFL039C ACT1 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MEMBRANE ORGANIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPON 0.0664069324986104 0.000119030688076403 457 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions ascospore wall assembly|vacuole inheritance|protein secretion|DNA repair|budding cell isotropic bud growth|mitochondrion inheritance|fungal-type cell wall organization|vesicle transport along actin filament|establishment of cell polarity|chronological cell aging|cytokinesis|actin filament reorganization involved in cell cycle|histone acetylation|endocytosis|exocytosis|establishment of mitotic spindle orientation|regulation of transcription from RNA polymerase II promoter|actomyosin contractile ring contraction|cellular response to oxidative stress structural constituent of cytoskeleton cellular bud neck contractile ring|actin filament|histone acetyltransferase complex|Swr1 complex|actin filament bundle|NuA4 histone acetyltransferase complex|Ino80 complex|actin cortical patch YIL065C FIS1 hom FT MITOCHONDRION ORGANIZATION|PEROXISOME ORGANIZATION|MITOCHONDRION 0.0663928536441464 0.000119428495433991 458 Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation peroxisome fission|mitochondrial fission|chronological cell aging|peroxisome organization molecular_function mitochondrial outer membrane|mitochondrion|peroxisome YGL055W OLE1 het FF|FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0663847870171018 0.000119656987622924 459 Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria unsaturated fatty acid biosynthetic process electron carrier activity|stearoyl-CoA 9-desaturase activity integral to endoplasmic reticulum membrane|integral to membrane YCL058C FYV5 hom FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0663760271457786 0.000120186672287427 460 Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis pheromone-dependent signal transduction involved in conjugation with cellular fusion|cellular ion homeostasis molecular_function integral to membrane YOR012W_p YOR012W hom 0.066268986313014 0.000124136602012352 461 Putative protein of unknown function biological_process molecular_function cellular_component YFL001W DEG1 hom FT RNA PROCESSING|NUCLEUS 0.0662214931291978 0.000127009040325765 462 tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity nucleus|cytoplasm YLR221C RSA3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0660584026190096 0.000129255322189146 463 Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosomal large subunit assembly molecular_function nucleolus|preribosome, large subunit precursor YDR448W ADA2 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0658524703242109 0.000135679729646139 464 Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes chromatin silencing at rDNA|chromatin modification|regulation of transcription from RNA polymerase II promoter|positive regulation of histone acetylation|chromatin silencing at telomere chromatin binding|transcription coactivator activity|histone acetyltransferase activity|phosphatidylserine binding SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YAR003W SWD1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0657758035402004 0.000138147386633873 465 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 histone H3-K4 methylation|telomere maintenance|regulation of transcription, DNA-dependent|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YDL213C NOP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0656432044874557 0.000142515349273329 466 rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes ribosomal small subunit biogenesis RNA binding|rRNA binding nucleolus|90S preribosome YNL297C MON2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0656086711024065 0.000144003516875184 467 Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins transport|endocytosis|protein targeting to vacuole|Golgi to endosome transport guanyl-nucleotide exchange factor activity cytosol|endosome|trans-Golgi network|extrinsic to membrane YOR183W_p FYV12 hom 0.0655956213731753 0.000144114245340087 468 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cellular_component YBR091C TIM12 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0654708293673587 0.00014838811114549 469 Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins protein import into mitochondrial inner membrane phospholipid binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YBL092W RPL32 het FF|FT TRANSLATION|RIBOSOME 0.0654479302664572 0.000151585118058862 470 Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR299W BUD7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0652274952004732 0.000158496702586429 471 Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process|cellular bud site selection molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YOR377W ATF1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM 0.0650243559001357 0.00016467585344382 472 Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Unknown|fermentation alcohol O-acetyltransferase activity lipid particle YAL048C GEM1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0650217128819422 0.000164777097228231 473 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion inheritance|regulation of mitochondrion organization|phospholipid homeostasis GTPase activity|calcium ion binding mitochondrial outer membrane|ERMES complex|mitochondrion YGL203C KEX1 hom FT PROTEOLYSIS|GOLGI APPARATUS 0.0649658174847266 0.000166931991488248 474 Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins protein processing serine-type carboxypeptidase activity trans-Golgi network YHR111W UBA4 hom FT RNA PROCESSING|CELL DIVISION|RESPONSE TO OXIDATIVE STRESS 0.064919506884346 0.000169877679409159 475 Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p cellular response to oxidative stress|tRNA wobble position uridine thiolation|cell budding|protein urmylation|tRNA thio-modification|Unknown|invasive growth in response to glucose limitation URM1 activating enzyme activity|sulfurtransferase activity|thiosulfate sulfurtransferase activity|protein adenylyltransferase activity cytoplasm YJR060W CBF1 hom FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME|KINETOCHORE 0.0648800450416949 0.000170290308943029 476 Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress regulation of transcription from RNA polymerase II promoter in response to methionine|negative regulation of transcription from RNA polymerase II promoter|methionine biosynthetic process|chromosome segregation|Unknown|chromatin remodeling|negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity|RNA polymerase II repressing transcription factor binding|centromeric DNA binding chromosome, centromeric region|mitochondrion|nucleus|kinetochore YDR310C SUM1 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS 0.0646371073377167 0.000180150275253768 477 Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint negative regulation of transcription by competitive promoter binding|negative regulation of transcription from RNA polymerase II promoter during mitosis|DNA replication initiation|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus YMR230W RPS10B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0645786001904156 0.000182603766148327 478 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YPL249C-A RPL36B hom FT TRANSLATION|RIBOSOME 0.0644720188966132 0.000187154137912036 479 Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDR053W_d YDR053W het 0.0644376287284819 0.000188644978315154 480 Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Unknown Unknown Unknown YDR264C AKR1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0643153584763245 0.000197926578657626 481 Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication protein targeting to membrane|regulation of endocytosis|regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus|membrane YLR292C SEC72 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0643076449588434 0.000194381257060638 482 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation protein transporter activity Sec62/Sec63 complex|endoplasmic reticulum membrane YOR216C RUD3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|GOLGI APPARATUS 0.0641694175917563 0.000200660853323586 483 Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus YCR059C YIH1 hom FT TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0640980544044337 0.000207584623381857 484 Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT negative regulation of protein phosphorylation protein kinase inhibitor activity|ribosome binding|actin monomer binding ribosome|nucleus|polysome|cytoplasm YNL042W BOP3 hom FT NUCLEUS 0.0640973585820277 0.000205805646393732 485 Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury cellular response to methylmercury molecular_function nucleus|cytoplasm YOR298C-A MBF1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0640264533069331 0.000207809658213471 486 Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity mitochondrion|nucleus YPR181C SEC23 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0635524144600585 0.000231085593190843 487 GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p regulation of COPII vesicle coating GTPase activator activity COPII vesicle coat YMR210W YMR210W hom FT KETONE METABOLISM|LIPID METABOLISM 0.0634331280997792 0.000237445948787008 488 Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification medium-chain fatty acid biosynthetic process transferase activity, transferring acyl groups cellular_component YCR087C-A_p YCR087C-A hom FT NUCLEUS 0.0633833753452378 0.000240146983959419 489 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene biological_process molecular_function nucleolus YOR236W DFR1 het FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0633499630801523 0.000241977051494315 490 Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism tetrahydrofolate biosynthetic process|dihydrofolate metabolic process dihydrofolate reductase activity|mRNA binding cytosol YMR073C IRC21 hom FF 0.0632907991302343 0.00024948368089823 491 Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study response to drug molecular_function cytoplasm YPL238C_d YPL238C het FF 0.0631471765920516 0.000254992140449071 492 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Unknown Unknown Unknown YLL039C UBI4 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0631406483556332 0.000253742618708753 493 Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress protein deubiquitination|ascospore formation|protein monoubiquitination|protein polyubiquitination|protein ubiquitination|response to stress ATP-dependent protein binding|protein tag cytoplasm YGR270W YTA7 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0630021460137686 0.000261820376607138 494 Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p negative regulation of transcription from RNA polymerase II promoter|negative regulation of chromatin silencing|positive regulation of transcription from RNA polymerase II promoter chromatin binding|histone binding extrinsic to endoplasmic reticulum membrane|nucleus YGR252W GCN5 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0628889276824861 0.000268601877179329 495 Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation histone acetylation|regulation of transcription by chromatin organization|chromatin modification|positive regulation of transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|transcription coactivator activity|histone acetyltransferase activity|H3 histone acetyltransferase activity SLIK (SAGA-like) complex|chromosome, centromeric region|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YLR443W ECM7 hom FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS 0.062853460617879 0.000270759893929367 496 Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency fungal-type cell wall organization|calcium ion transport molecular_function integral to membrane YJR125C ENT3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|CYTOSKELETON 0.0627786135644816 0.000275367432965574 497 Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p late endosome to vacuole transport via multivesicular body sorting pathway|early endosome to Golgi transport|actin filament organization|endocytosis|actin cortical patch assembly|Golgi to endosome transport protein binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding clathrin vesicle coat|actin cortical patch YLR048W RPS0B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.062759474505135 0.000276557358558814 498 Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 cytoplasmic translation|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA export from nucleus|ribosomal small subunit assembly|endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YCL045C EMC1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0626774004907621 0.000281714940298946 499 Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YHR123W EPT1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0622760529362444 0.000308259037444032 500 sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolaminephosphotransferase activity|diacylglycerol cholinephosphotransferase activity integral to membrane|Golgi apparatus YHR186C KOG1 het FT SIGNALING|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION|PLASMA MEMBRANE 0.0620689469448571 0.000322855166589069 501 Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors TOR signaling cascade|regulation of cell growth|cellular response to starvation ubiquitin binding TORC1 complex|fungal-type vacuole membrane|mitochondrion|plasma membrane YDL171C GLT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.062067400382924 0.000322966555915135 502 NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source glutamate biosynthetic process|ammonia assimilation cycle glutamate synthase (NADH) activity mitochondrion YLL012W YEH1 hom FT LIPID METABOLISM 0.0620607898166605 0.000323443079672846 503 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes sterol metabolic process sterol esterase activity integral to membrane|lipid particle YJL024C APS3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0620142342250772 0.000326817721460812 504 Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YPL239W YAR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS 0.0618445178457625 0.000339400747976364 505 Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock ribosomal small subunit biogenesis|cellular response to oxidative stress|response to osmotic stress molecular_function cytoplasm YDR102C_d YDR102C hom 0.0618187450531953 0.000341350743123216 506 Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Unknown Unknown Unknown YMR133W REC114 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0617300947971412 0.000348138396497319 507 Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YGL054C ERV14 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0616884312652626 0.000359339524517145 508 COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication ascospore formation|ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle|endoplasmic reticulum membrane YBR181C RPS6B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0616063538466953 0.00035782403238814 509 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|cytoplasm YHR117W TOM71 hom FT MITOCHONDRION 0.0614685609428639 0.00036890570338227 510 Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication biological_process protein transporter activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane YMR100W MUB1 hom FT CELL DIVISION 0.0614595709711573 0.000373399614348597 511 MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene regulation of cell budding|protein monoubiquitination molecular_function cellular_component YPR034W ARP7 het FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0613656102351973 0.000377393709636076 512 Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation nucleosome mobilization|ATP-dependent chromatin remodeling|chromatin organization|nucleosome disassembly|transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA translocase activity RSC complex|SWI/SNF complex|nucleus YBR286W APE3 hom FT PROTEOLYSIS 0.0611885493784272 0.000392420040381728 513 Vacuolar aminopeptidase Y, processed to mature form by Prb1p vacuolar protein catabolic process aminopeptidase activity fungal-type vacuole YNR040W_p YNR040W hom FT MITOCHONDRION 0.0611480662061699 0.000396727788274117 514 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YLR049C_p YLR049C hom 0.0611430159876178 0.000396373590696193 515 Putative protein of unknown function biological_process molecular_function cellular_component YOL012C HTZ1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0611213739320507 0.000398265729975832 516 Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin nuclear-transcribed mRNA catabolic process, non-stop decay|regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette chromatin binding nuclear chromatin|chromatin assembly complex YBR171W SEC66 hom FF|FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0611197494886463 0.000400809313384396 517 Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation|filamentous growth protein transporter activity integral to membrane|Sec62/Sec63 complex|endoplasmic reticulum membrane YLR259C HSP60 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0611119437607186 0.000399092834297931 518 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated protein refolding|protein stabilization|chaperone-mediated protein complex assembly|'de novo' protein folding|protein maturation|protein import into mitochondrial intermembrane space unfolded protein binding|DNA replication origin binding|single-stranded DNA binding|ATPase activity|chaperone binding mitochondrial nucleoid|mitochondrion YMR139W RIM11 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION 0.0611050590039646 0.00039969769737652 519 Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress proteolysis|ascospore formation|protein phosphorylation|response to stress protein serine/threonine kinase activity|protein kinase activity cytoplasm YPL152W RRD2 hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION 0.0610960799264549 0.000400487844200573 520 Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress mitotic spindle organization in nucleus|response to osmotic stress peptidyl-prolyl cis-trans isomerase activity|protein phosphatase type 2A regulator activity protein phosphatase type 2A complex YMR132C_p JLP2 hom 0.0609432664262893 0.000419976883345836 521 Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) biological_process molecular_function cytoplasm YBR115C LYS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0607037827265992 0.00043646824441366 522 Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p lysine biosynthetic process via aminoadipic acid L-aminoadipate-semialdehyde dehydrogenase activity cytoplasm YPL011C TAF3 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0606860294378027 0.000438165782658631 523 TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly transcription factor TFIID complex YDR140W MTQ2 hom FT TRANSLATION|NUCLEUS 0.0606187465958622 0.000444655323026162 524 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC peptidyl-glutamine methylation|regulation of translation protein methyltransferase activity|S-adenosylmethionine-dependent methyltransferase activity eRF1 methyltransferase complex|nucleus|cytoplasm YPL036W PMA2 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0605826877728957 0.000448170053183009 525 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential proton transport|regulation of pH hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YER030W CHZ1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0604702263432816 0.000459299081937432 526 Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin remodeling histone binding chromatin assembly complex|nucleus YNR039C ZRG17 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0603276069993831 0.000473783277851657 527 Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency zinc ion transport zinc ion transmembrane transporter activity|metal ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YBR084W MIS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0602515319078785 0.000481682434087888 528 Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase nucleobase-containing compound metabolic process|folic acid biosynthetic process formate-tetrahydrofolate ligase activity|methylenetetrahydrofolate dehydrogenase (NADP+) activity|methenyltetrahydrofolate cyclohydrolase activity mitochondrion YMR077C VPS20 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.060087632037731 0.000502032734297125 529 Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport protein binding ESCRT III complex|cytoplasm YLL036C PRP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0600708703002109 0.000500935843531292 530 Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain generation of catalytic spliceosome for first transesterification step ubiquitin-protein ligase activity|first spliceosomal transesterification activity mitochondrion|nucleus|Prp19 complex|cytoplasm|U2-type catalytic step 1 spliceosome YGR262C BUD32 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|LIPID METABOLISM|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.0600575371936468 0.000502384823673119 531 Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription threonylcarbamoyladenosine metabolic process|protein phosphorylation|telomere maintenance|cellular bud site selection|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity EKC/KEOPS complex|nucleus|cytoplasm YDL008W APC11 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0600559437734708 0.000502558250225664 532 Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YPL042C SSN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0600447029221013 0.000506719487336871 533 Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression phosphorylation of RNA polymerase II C-terminal domain|negative regulation of transcription from RNA polymerase II promoter|negative regulation of filamentous growth|protein phosphorylation|protein destabilization|nuclear-transcribed mRNA catabolic process, non-stop decay|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II carboxy-terminal domain kinase activity|protein kinase activity|cyclin-dependent protein kinase activity mediator complex YOR312C RPL20B hom FT TRANSLATION|RIBOSOME 0.060040926406153 0.000504195471133548 534 Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YHR077C NMD2 hom 0.0599598343430349 0.000513122492736292 535 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|translational frameshifting molecular_function polysome|cytoplasm YLR179C YLR179C hom FT NUCLEUS 0.0599438320575262 0.000514901411682746 536 Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YMR063W RIM9 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0597994557710316 0.000542565928175813 537 Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; ascospore formation molecular_function integral to membrane YDR036C EHD3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0597656469384464 0.000535101655357625 538 3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis fatty acid beta-oxidation|biological_process 3-hydroxyisobutyryl-CoA hydrolase activity mitochondrion YFL033C RIM15 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0596836729723371 0.000550935611296575 539 Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase protein phosphorylation|age-dependent response to oxidative stress involved in chronological cell aging|response to stress|regulation of meiosis protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YOL141W PPM2 hom FT RNA PROCESSING|MITOCHONDRION 0.0596662662838153 0.000546686319907807 540 AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA methylation|wybutosine biosynthetic process tRNA methyltransferase activity mitochondrion|cytoplasm YOL117W RRI2 hom FT SIGNALING|NUCLEUS 0.0595991968215512 0.000555696490763544 541 Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YJR106W ECM27 hom FT CELL WALL ORG/BIOGENESIS 0.0595574221329833 0.000559641843318378 542 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p fungal-type cell wall organization molecular_function integral to membrane YLR326W_p YLR326W hom 0.0595368016121055 0.000562128245039646 543 Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cellular_component YGL148W ARO2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0595212634651667 0.000567241970307216 544 Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress aromatic amino acid family biosynthetic process chorismate synthase activity|riboflavin reductase (NADPH) activity cytoplasm YGR155W CYS4 hom FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|MITOCHONDRION 0.0594659965227069 0.000580599827471929 545 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria cysteine biosynthetic process from serine|hydrogen sulfide biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|traversing start control point of mitotic cell cycle cystathionine beta-synthase activity mitochondrion|cytoplasm YEL063C CAN1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0594022922819743 0.000580802163913423 546 Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication basic amino acid transport|transmembrane transport basic amino acid transmembrane transporter activity|arginine transmembrane transporter activity mitochondrion|integral to membrane|membrane raft|plasma membrane YML014W TRM9 hom FT RNA PROCESSING|NUCLEUS 0.0593454146509335 0.000585701153425541 547 tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses chronological cell aging|tRNA methylation|tRNA wobble uridine modification|response to stress tRNA (uracil) methyltransferase activity nucleus|cytoplasm YDR349C YPS7 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0590578701871967 0.000622852099378852 548 Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum fungal-type cell wall organization aspartic-type endopeptidase activity endoplasmic reticulum|cytoplasm YJL159W HSP150 hom FT CELL WALL ORG/BIOGENESIS 0.0590123142981319 0.000628935236390547 549 O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication fungal-type cell wall organization structural constituent of cell wall|ATPase activity fungal-type cell wall|extracellular region YDR434W GPI17 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0590121043215262 0.000628963402309946 550 Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|endoplasmic reticulum|GPI-anchor transamidase complex YGR164W_d YGR164W hom 0.0588654088062094 0.000648930105691334 551 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL105W ARC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0587850882000035 0.000660110612373459 552 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress positive regulation of ligase activity|tRNA aminoacylation for protein translation enzyme activator activity|tRNA binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding methionyl glutamyl tRNA synthetase complex|cytoplasm YGL081W_p YGL081W hom 0.0587517204502228 0.000664807779313566 553 Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis biological_process molecular_function cellular_component YBR191W RPL21A hom FT TRANSLATION|RIBOSOME 0.0587139321657837 0.000670164723514214 554 Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR008C-A_p YOR008C-A hom 0.0586689223735376 0.000680372555621659 555 Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres biological_process molecular_function cellular_component YAL047C SPC72 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0584997055751964 0.000701299909899893 556 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization microtubule nucleation|nuclear migration along microtubule|mitotic cell cycle spindle orientation checkpoint|mitotic spindle elongation|mitotic sister chromatid segregation structural constituent of cytoskeleton outer plaque of spindle pole body YNR020C ATP23 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0583732864473933 0.000720298389151338 557 Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p protein processing|mitochondrial proton-transporting ATP synthase complex assembly metalloendopeptidase activity mitochondrial inner membrane|mitochondrial intermembrane space YML128C MSC1 hom FT CELL CYCLE|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0583117827797598 0.000729713083762405 558 Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated reciprocal meiotic recombination molecular_function mitochondrion|endoplasmic reticulum|plasma membrane YJR145C RPS4A hom FT TRANSLATION|RIBOSOME 0.0582269497696594 0.000746972574712503 559 Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasm|90S preribosome YDR351W SBE2 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|GOLGI APPARATUS 0.0581688669204797 0.000752032933025855 560 Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth fungal-type cell wall organization molecular_function Golgi apparatus YDL190C UFD2 hom FT PROTEOLYSIS|NUCLEUS 0.058163568539854 0.000752872462363522 561 Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 ER-associated protein catabolic process|protein ubiquitination|ubiquitin-dependent protein catabolic process|response to stress ubiquitin-ubiquitin ligase activity nucleus|cytoplasm YDR190C RVB1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0581105976180475 0.000766881070011757 562 ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly regulation of transcription from RNA polymerase II promoter|box C/D snoRNP assembly|chromatin remodeling|histone exchange ATP-dependent 5'-3' DNA helicase activity|ATP-dependent 3'-5' DNA helicase activity ASTRA complex|R2TP complex|Swr1 complex|nucleus|Ino80 complex YML073C RPL6A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0580091797920395 0.000783389456475628 563 Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDR057W YOS9 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0579781622182975 0.00078280381463398 564 ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family ER-associated protein catabolic process oligosaccharide binding luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum lumen YOR340C RPA43 het FF|FT NUCLEUS 0.0578188882153546 0.000809394188538983 565 RNA polymerase I subunit A43 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex YMR290W-A_d YMR290W-A het FF 0.0577726373498645 0.000817271172468975 566 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Unknown Unknown Unknown YAL058W CNE1 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0577371709443295 0.000830801983641403 567 Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast protein folding|ER-associated protein catabolic process unfolded protein binding integral to endoplasmic reticulum membrane YCR102W-A_d YCR102W-A hom 0.0576902035065441 0.00083148700526434 568 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR118W TEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|CYTOSKELETON|RIBOSOME 0.0575057037504391 0.000864139067065743 569 Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication translational elongation|tRNA export from nucleus GDP binding|GTP binding|translation elongation factor activity ribosome|eukaryotic translation elongation factor 1 complex|cytoplasm YDL065C PEX19 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0574979953796924 0.000870178002396919 570 Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning protein stabilization|protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization|protein exit from endoplasmic reticulum peroxisome membrane targeting sequence binding|protein binding cytosol|peroxisomal membrane|endoplasmic reticulum YLR386W VAC14 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM 0.0574979033796518 0.000865545386423115 571 Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p phosphatidylinositol biosynthetic process|positive regulation of kinase activity protein binding, bridging fungal-type vacuole membrane|PAS complex|extrinsic to vacuolar membrane YOL160W_d YOL160W hom 0.0574762713993523 0.000875684780703371 572 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR062C_p YOR062C hom FT NUCLEUS 0.0574074977472945 0.000881999999880932 573 Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YGL011C SCL1 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0571153932454929 0.000937167731137772 574 Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|nucleus|proteasome storage granule|nuclear outer membrane-endoplasmic reticulum membrane network YGR214W RPS0A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0570204128396618 0.000962528393121038 575 Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; cytoplasmic translation|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA export from nucleus|ribosomal small subunit assembly|endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YBL006C LDB7 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CELL WALL ORG/BIOGENESIS|NUCLEUS|RSC COMPLEX 0.0569880417925177 0.000963897049446292 576 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions ATP-dependent chromatin remodeling|cell wall mannoprotein biosynthetic process|nucleosome disassembly|transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YGL246C RAI1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0568782241092476 0.00101047293785122 577 Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z deadenylation-dependent decapping of nuclear-transcribed mRNA|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|termination of RNA polymerase II transcription, poly(A)-coupled|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) enzyme regulator activity|m7G(5')pppN diphosphatase activity nucleus YPL106C SSE1 hom 0.0567354301003476 0.00101371303251329 578 ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication protein folding|protein refolding peptide binding|adenyl-nucleotide exchange factor activity|ATP binding polysome|cytoplasm YJR018W_d YJR018W hom 0.0566984771744096 0.00102145807001565 579 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL122W UBP1 hom FT PROTEOLYSIS|ENDOPLASMIC RETICULUM 0.0566019344380911 0.00106201799118608 580 Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains protein deubiquitination ubiquitin-specific protease activity endoplasmic reticulum|cytoplasm YNL256W FOL1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0565810997083719 0.00104642404230563 581 Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities tetrahydrofolate biosynthetic process dihydroneopterin aldolase activity|2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity|dihydropteroate synthase activity mitochondrial envelope|mitochondrion|cytoplasm YHR131C_p YHR131C hom 0.0564792875523646 0.00107966488793863 582 Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study biological_process molecular_function cytoplasm YOR246C ENV9 hom FF|FT OXIDATION-REDUCTION PROCESS|VACUOLAR PROTEIN PROCESSING 0.0564187799874717 0.00108936403258949 583 Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts vacuole organization|vacuolar protein processing oxidoreductase activity lipid particle YBR106W PHO88 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0564041390749292 0.00108513176430852 584 Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations phosphate ion transport molecular_function mitochondrion|membrane YNL197C WHI3 hom FT PROTEIN LOCALIZATION|CELL CYCLE 0.0564016076146549 0.00108756666903514 585 RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication pseudohyphal growth|invasive growth in response to glucose limitation|regulation of cell size|cytoplasmic sequestering of protein RNA binding cytoplasm YOR105W_p YOR105W hom 0.0563638558188859 0.00109412617876342 586 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YKL183W LOT5 hom FT NUCLEUS 0.0562850553047133 0.00111382978242804 587 Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YHR167W THP2 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0561963680197492 0.00113226661500792 588 Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance mRNA export from nucleus|transcription elongation from RNA polymerase II promoter|DNA recombination nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YLR264W RPS28B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0561709961048708 0.00113815032634801 589 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YHR140W_p YHR140W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0561630207652835 0.00114000562392883 590 Putative integral membrane protein of unknown function biological_process molecular_function integral to membrane YDL174C DLD1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0560583099297369 0.00116462501971154 591 D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane cellular carbohydrate metabolic process|aerobic respiration D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial inner membrane YBR298C MAL31 hom FT CARBOHYDRATE METABOLISM 0.0560434628102727 0.00117813971022968 592 Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C alpha-glucoside transport|transmembrane transport alpha-glucoside:hydrogen symporter activity integral to membrane|membrane YLR307W CDA1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0560266084716309 0.00118621740082126 593 Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall ascospore wall assembly chitin deacetylase activity chitosan layer of spore wall YHR101C BIG1 het FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0560054844042386 0.0011772307749824 594 Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan fungal-type cell wall biogenesis molecular_function integral to endoplasmic reticulum membrane YKL082C RRP14 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.0558779482170931 0.0012081859159872 595 Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family ribosomal small subunit biogenesis|ribosomal large subunit biogenesis molecular_function cytosolic large ribosomal subunit|nucleolus YML117W NAB6 hom 0.0557422782961931 0.00125667567054504 596 Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP biological_process molecular_function cytoplasm YFR037C RSC8 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|RSC COMPLEX 0.0557097638666484 0.00125015641130982 597 Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters ATP-dependent chromatin remodeling|double-strand break repair via nonhomologous end joining|nucleosome disassembly|regulation of transcription, DNA-dependent|transcription elongation from RNA polymerase II promoter|G1/S transition of mitotic cell cycle DNA translocase activity|DNA binding RSC complex|nucleus YGR285C ZUO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0553746547653053 0.00133782230171181 598 Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p protein folding|ribosomal subunit export from nucleus|rRNA processing|regulation of translational fidelity unfolded protein binding mitochondrion|ribosome|nucleolus|polysome|cytoplasm YGR208W SER2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0553714001276652 0.00134764221730837 599 Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source L-serine biosynthetic process phosphoserine phosphatase activity nucleus|cytoplasm YGL137W SEC27 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0553173999293856 0.00135335559821592 600 Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP retrograde vesicle-mediated transport, Golgi to ER|late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YJL071W ARG2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0552302248037188 0.00137732590944119 601 Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p arginine biosynthetic process|ornithine biosynthetic process acetyl-CoA:L-glutamate N-acetyltransferase activity mitochondrial matrix YPL006W NCR1 hom FT LIPID METABOLISM 0.0551690853117426 0.0013966767078951 602 Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein sphingolipid metabolic process sphingolipid transporter activity fungal-type vacuole membrane|integral to membrane YDR500C RPL37B hom FT TRANSLATION|RIBOSOME 0.0551530853919382 0.00140117525836382 603 Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YER065C ICL1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.0551055614429951 0.00141228477996031 604 Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose glyoxylate cycle isocitrate lyase activity cellular_component YLR410W VIP1 hom FT CYTOSKELETON 0.0549905621689274 0.00144525685615938 605 Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 inositol phosphate biosynthetic process inositol hexakisphosphate 1-kinase activity|inositol hexakisphosphate 3-kinase activity|inositol heptakisphosphate kinase activity|inositol hexakisphosphate 4-kinase activity|inositol hexakisphosphate 6-kinase activity cytoplasm YNL305C BXI1 hom FT SIGNALING|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.054946249768585 0.0014581501103261 606 Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p endoplasmic reticulum unfolded protein response|apoptotic process|calcium-mediated signaling molecular_function mitochondrion|integral to membrane|endoplasmic reticulum|fungal-type vacuole YOL003C PFA4 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0549443933945187 0.0014586925512482 607 Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein palmitoylation palmitoyltransferase activity integral to membrane|endoplasmic reticulum YKL154W SRP102 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.054803079923308 0.00150053488202435 608 Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane protein targeting to ER|SRP-dependent cotranslational protein targeting to membrane signal recognition particle binding|GTP binding|GTPase activity integral to endoplasmic reticulum membrane|signal recognition particle receptor complex YNL107W YAF9 hom FF|FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.054705482910199 0.00153007520334016 609 Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain DNA repair|chromatin remodeling|histone exchange|chromatin silencing at telomere molecular_function Swr1 complex|nucleus|NuA4 histone acetyltransferase complex|cytoplasm YNL211C_p YNL211C hom FT MITOCHONDRION 0.0546573471655258 0.00154484106708542 610 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene biological_process molecular_function mitochondrion YGR020C VMA7 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0545895070516066 0.00156587411497487 611 Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane vacuolar acidification proton-transporting ATPase activity, rotational mechanism fungal-type vacuole membrane|vacuolar proton-transporting V-type ATPase, V1 domain|integral to membrane YGR170W PSD2 hom FT LIPID METABOLISM|GOLGI APPARATUS 0.0545875106348207 0.00156649705451713 612 Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine phosphatidylcholine biosynthetic process phosphatidylserine decarboxylase activity endosome YBR174C_d YBR174C hom 0.0545214800952756 0.00158722945945299 613 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Unknown Unknown Unknown YGR159C NSR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0543614758824679 0.00163851946829342 614 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis ribosomal small subunit assembly|rRNA processing single-stranded DNA binding|RNA binding mitochondrion|nucleolus|nucleus YBR058C UBP14 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.0542462124822734 0.00167640756018729 615 Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T protein deubiquitination|negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process ubiquitin-specific protease activity cytoplasm YBR207W FTH1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0540301942448844 0.00174959235707462 616 Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress endocytosis|high-affinity iron ion transport iron ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YKR008W RSC4 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0538869361888476 0.00179973396454488 617 Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|DNA translocase activity RSC complex YJL088W ARG3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0538746397429786 0.00180409877405616 618 Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine arginine biosynthetic process|ornithine metabolic process ornithine carbamoyltransferase activity cytosol YGR179C OKP1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0538541905541729 0.00181137906184888 619 Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 attachment of spindle microtubules to kinetochore|chromosome segregation molecular_function COMA complex|condensed nuclear chromosome kinetochore YJR014W TMA22 hom FT TRANSLATION|RIBOSOME 0.0537934966539491 0.00184765777430547 620 Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress biological_process RNA binding ribosome|cytoplasm YFR032C_p RRT5 hom 0.0537347408762455 0.00185444730939349 621 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function cellular_component YOR084W LPX1 hom FT PEROXISOME ORGANIZATION 0.0537125030966313 0.00187432123738353 622 Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction peroxisome organization lipase activity peroxisomal matrix YJR011C_p YJR011C hom 0.0536211983140099 0.00189625550966918 623 Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YFL032W_d YFL032W hom 0.0534333317874171 0.00196733544574594 624 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Unknown Unknown Unknown YDL053C PBP4 hom FT NUCLEUS 0.0533978863855196 0.00198101740417827 625 Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasmic stress granule|cytoplasm YFR006W_p YFR006W hom 0.0533876989627248 0.00198496587923612 626 Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene biological_process molecular_function cytoplasm YMR113W FOL3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0532499074175011 0.00203908561586358 627 Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis folic acid-containing compound biosynthetic process dihydrofolate synthase activity cytoplasm YLR185W RPL37A hom FT TRANSLATION|RIBOSOME 0.0531244515395107 0.00208953387153655 628 Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLL021W SPA2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0530267336236911 0.00212961810987134 629 Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate regulation of initiation of mating projection growth|regulation of termination of mating projection growth|pseudohyphal growth|invasive filamentous growth|mating projection assembly cytoskeletal regulatory protein binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip YFL048C EMP47 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.052969973640187 0.00215322362293002 630 Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport ER to Golgi vesicle-mediated transport glycoprotein binding fungal-type vacuole membrane|integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YAL056C-A_d YAL056C-A hom 0.0527763552247919 0.00223556175528273 631 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCR081W SRB8 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0524359804296511 0.00239812758388551 632 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription by galactose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity mediator complex YPR146C_d YPR146C hom 0.0523887231489665 0.0024091342039021 633 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR192W LNP1 hom 0.0523864726896361 0.00241017695101653 634 Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS endoplasmic reticulum tubular network organization molecular_function endoplasmic reticulum tubular network|cytoplasm YCR045C RRT12 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.052325580463009 0.00244220404506207 635 Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores ascospore wall assembly serine-type peptidase activity nuclear envelope|ascospore wall YDL136W RPL35B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.052219145826212 0.00250004203541599 636 Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YLR353W BUD8 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0521663962430346 0.0025141665297131 637 Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole pseudohyphal growth|cellular bud site selection molecular_function incipient cellular bud site|plasma membrane|cellular bud tip YKL179C COY1 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0521609282221328 0.00251680171423864 638 Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Golgi vesicle transport molecular_function Golgi membrane YDR161W_p YDR161W hom FT PROTEOLYSIS|NUCLEUS 0.0521306642523679 0.00253143244932992 639 Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) ER-associated protein catabolic process molecular_function nucleus|cytoplasm YGR001C_p YGR001C hom 0.0520341952761227 0.00259777561231749 640 Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae biological_process methyltransferase activity cytoplasm YOL038W PRE6 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0520154551018249 0.00258784309506776 641 Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YPR159W KRE6 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0519610743267133 0.00262261159337818 642 Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p fungal-type cell wall organization|(1->6)-beta-D-glucan biosynthetic process glucosidase activity integral to endoplasmic reticulum membrane|integral to membrane|site of polarized growth|transport vesicle|plasma membrane YGR254W ENO1 hom FT MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0518723905098261 0.00267127770691921 643 Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|gluconeogenesis|glycolysis phosphopyruvate hydratase activity mitochondrion|phosphopyruvate hydratase complex|plasma membrane|fungal-type vacuole|cytoplasm YER087C-A_d YER087C-A hom 0.0518109704090392 0.00269080331009317 644 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Unknown Unknown Unknown YOL006C TOP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0517394558998028 0.0027276856733128 645 Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination nuclear migration|chromatin assembly or disassembly|DNA topological change|DNA strand elongation involved in DNA replication|regulation of mitotic recombination|chromatin silencing at rDNA|regulation of transcription from RNA polymerase II promoter|mitotic chromosome condensation|transcription elongation from RNA polymerase II promoter DNA topoisomerase type I activity replication fork protection complex|nucleolus|nucleus YDR420W HKR1 hom FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0516840812643835 0.00276869521678495 646 Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection (1->3)-beta-D-glucan biosynthetic process|fungal-type cell wall organization|osmosensory signaling pathway via Sho1 osmosensor|hyperosmotic response|cellular bud site selection osmosensor activity site of polarized growth|plasma membrane YML063W RPS1B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0516358133110686 0.00278195728432237 647 Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YIL133C RPL16A hom FT TRANSLATION|RIBOSOME 0.0516075027274633 0.00279695235444963 648 Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YOL139C CDC33 het FT CELL CYCLE|TRANSLATION|NUCLEUS|RIBOSOME 0.0515797304496943 0.00281173395964591 649 mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth regulation of cell cycle|translational initiation|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay phosphatidylinositol-3-phosphate binding|translation initiation factor activity ribosome|nucleus|eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule|cytoplasm YGL094C PAN2 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEOLYSIS 0.0515389712859567 0.00283355689208844 650 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes mRNA 3'-end processing|postreplication repair|mRNA processing poly(A)-specific ribonuclease activity PAN complex|cytoplasm YOR163W DDP1 hom FT CARBOHYDRATE METABOLISM|NUCLEUS 0.0515331888712391 0.00283666535465097 651 Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress polyphosphate catabolic process|inositol phosphate dephosphorylation|diadenosine polyphosphate catabolic process diphosphoinositol-polyphosphate diphosphatase activity|endopolyphosphatase activity|bis(5'-adenosyl)-pentaphosphatase activity|bis(5'-adenosyl)-hexaphosphatase activity nucleus|cytoplasm YKR023W_p YKR023W hom FT NUCLEUS|MITOCHONDRION 0.0515099371086939 0.00284919628814782 652 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YGL084C GUP1 hom FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0514585655666608 0.0028770609353362 653 Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport GPI anchor biosynthetic process|glycerol transport|glycerol catabolic process O-acyltransferase activity integral to membrane|endoplasmic reticulum|plasma membrane|membrane YEL042W GDA1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0513614861650599 0.00293039812346841 654 Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate protein glycosylation guanosine-diphosphatase activity|uridine-diphosphatase activity Golgi apparatus YDR241W_d BUD26 hom 0.0512364583512703 0.00300041930240062 655 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YJL029C VPS53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0511318804782022 0.00306015568563514 656 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Golgi to vacuole transport|retrograde transport, endosome to Golgi molecular_function Golgi apparatus|GARP complex|cytoplasm YAL034C-B_d YAL034C-B het 0.0511088762815729 0.00307344062796352 657 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR061W RPL22A hom FT TRANSLATION|RIBOSOME 0.0511065400144015 0.00309248075942907 658 Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR017W MEU1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0510540917962657 0.00310529064719874 659 Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression L-methionine salvage from methylthioadenosine|glutamate biosynthetic process S-methyl-5-thioadenosine phosphorylase activity|mRNA binding cytoplasm YHR122W CIA2 het FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS 0.0510299949990882 0.00311939480262246 660 Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B establishment of mitotic sister chromatid cohesion molecular_function nucleus|cytoplasm YMR262W_p YMR262W hom 0.0509019781347306 0.00319530833339313 661 Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component YBR029C CDS1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0508959182027283 0.00319894322222567 662 Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids phosphatidylserine metabolic process|phosphatidylglycerol biosynthetic process phosphatidate cytidylyltransferase activity mitochondrion|integral to membrane|endoplasmic reticulum YDL229W SSB1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RESPONSE TO NUTRIENT LEVELS 0.0507039439296463 0.00331606176156091 663 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication cytoplasmic translation|cellular response to glucose starvation|translational termination|ribosomal subunit export from nucleus|regulation of translational fidelity|rRNA processing|'de novo' cotranslational protein folding unfolded protein binding|ATPase activity|calmodulin binding plasma membrane|polysome|cytoplasm YPR133W-A TOM5 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0507028403242581 0.00333083717619429 664 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore mitochondrial outer membrane translocase complex assembly|protein targeting to mitochondrion protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YHR166C CDC23 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|CHROMOSOME|UBIQUITIN LIGASE COMPLEX 0.0506316526343576 0.00336116722244199 665 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity|cyclin binding anaphase-promoting complex YDR336W_p YDR336W hom 0.0505476489028729 0.00344319014015346 666 Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene biological_process molecular_function cellular_component YPR152C URN1 hom FT RNA PROCESSING|NUCLEUS 0.0504299530421233 0.00348998398647165 667 Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase biological_process molecular_function nucleus YMR078C CTF18 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0504269761209264 0.00349191839809708 668 Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint maintenance of DNA trinucleotide repeats|double-strand break repair via homologous recombination|DNA replication initiation|mitotic sister chromatid cohesion molecular_function mitochondrion|nuclear replication fork|Ctf18 RFC-like complex YOR104W PIN2 hom 0.0503608976537903 0.00353510823362668 669 Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated biological_process molecular_function cellular_component YJR092W BUD4 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0503495808100248 0.00354255362385037 670 Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p cellular bud site selection|axial cellular bud site selection GTP binding cellular bud neck contractile ring YOL075C_p YOL075C hom FT NUCLEOTIDE METABOLISM 0.050322068932135 0.0035607132916079 671 Putative ABC transporter transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YFR051C RET2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0502092769491981 0.00363605173912412 672 Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER|Golgi inheritance|ER to Golgi vesicle-mediated transport|Golgi localization molecular_function COPI vesicle coat YBR214W SDS24 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION 0.0501927171000516 0.00364723391863986 673 Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication endocytosis|cytokinetic cell separation molecular_function cytoplasm YLR378C SEC61 het FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.050166216845427 0.00366519349019799 674 Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER posttranslational protein targeting to membrane, translocation|retrograde protein transport, ER to cytosol|ER-associated protein catabolic process|SRP-dependent cotranslational protein targeting to membrane, translocation|peptide transport peptide transporter activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity|signal sequence binding integral to membrane|Sec61 translocon complex YKL025C PAN3 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION 0.0500702192662236 0.00373092729213333 675 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes DNA repair|mRNA 3'-end processing|postreplication repair poly(A)-specific ribonuclease activity PAN complex|cytoplasm YMR239C RNT1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS 0.0500066591244813 0.00377503722635836 676 Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes U5 snRNA 3'-end processing|mRNA cleavage|chromatin organization|termination of RNA polymerase II transcription, exosome-dependent|U4 snRNA 3'-end processing|U1 snRNA 3'-end processing|rRNA transcription|rRNA processing ribonuclease III activity nucleoplasm|nucleolus YML115C VAN1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0500034486654255 0.00377727776217969 677 Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant protein N-linked glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YLR448W RPL6B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.049956906886794 0.00382564312200001 678 Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDL130W RPP1B hom FT TRANSLATION|RIBOSOME 0.0499285034432105 0.00386162125970462 679 Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component cytoplasmic translation|translational elongation structural constituent of ribosome cytosolic large ribosomal subunit YKL010C UFD4 hom FT PROTEOLYSIS|MITOCHONDRION 0.0497860969034017 0.00395875759034136 680 Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress protein monoubiquitination|protein polyubiquitination ubiquitin-protein ligase activity mitochondrion|cytoplasm YEL060C PRB1 hom FT PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS 0.0497593853489709 0.00395118485712863 681 Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress vacuolar protein catabolic process|sporulation resulting in formation of a cellular spore|cellular response to starvation serine-type endopeptidase activity fungal-type vacuole lumen|fungal-type vacuole YDL115C IWR1 hom FT PROTEIN LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0496633494491158 0.00402158484815946 682 RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress cellular protein complex localization|nucleocytoplasmic transport protein binding nucleus|DNA-directed RNA polymerase II, holoenzyme|cytoplasm YPL180W TCO89 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0496591046532904 0.00402472264014071 683 Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin TOR signaling cascade|fungal-type cell wall organization|response to salt stress|regulation of cell growth|glycerol metabolic process molecular_function TORC1 complex|fungal-type vacuole membrane|extrinsic to internal side of plasma membrane|extrinsic to vacuolar membrane YDL071C_d YDL071C hom 0.0496314765097221 0.00406724262456284 684 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Unknown Unknown Unknown YFL009W CDC4 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0496097817743679 0.00406686253378875 685 F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p meiosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity|ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus|nuclear matrix YJL143W TIM17 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0495862113757953 0.00407895378739298 686 Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex protein import into mitochondrial matrix|mitochondrial genome maintenance protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane presequence translocase complex YNR050C LYS9 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0495796188025688 0.00408389107324686 687 Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 lysine biosynthetic process via aminoadipic acid saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity cytoplasm YOR179C SYC1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0495344743681964 0.00411784641008964 688 Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication termination of RNA polymerase II transcription, exosome-dependent|termination of RNA polymerase II transcription, poly(A)-coupled RNA binding mRNA cleavage and polyadenylation specificity factor complex|nucleus YKL117W SBA1 hom FT NUCLEUS 0.0495029196423814 0.00414733571656713 689 Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress protein folding|positive regulation of telomere maintenance via telomerase|regulation of telomerase activity chaperone binding nucleus|cytoplasm YOR130C ORT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0494682521355385 0.00419635663841048 690 Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome arginine biosynthetic process|mitochondrial ornithine transport L-ornithine transmembrane transporter activity mitochondrial envelope|integral to membrane YNL067W RPL9B hom FT TRANSLATION|RIBOSOME 0.0494184106295663 0.00420632226004484 691 Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YML112W CTK3 hom FF|FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0493786128014918 0.00427140900505852 692 Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress mRNA 3'-end processing|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation cyclin-dependent protein kinase activity nucleoplasm|nucleolus|carboxy-terminal domain protein kinase complex YOR304W ISW2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0493616912877896 0.00425018355918614 693 ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth negative regulation of transcription from RNA polymerase II promoter by pheromones|nucleosome positioning|termination of RNA polymerase II transcription|negative regulation of antisense RNA transcription|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere single-stranded DNA binding|DNA translocase activity chromatin accessibility complex|nucleus YBL071W-A KTI11 hom FT RNA PROCESSING|NUCLEUS 0.0492521971398897 0.00434766214052896 694 Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs peptidyl-diphthamide biosynthetic process from peptidyl-histidine|tRNA wobble uridine modification electron carrier activity|iron ion binding|zinc ion binding nucleus|cytoplasm YDR017C KCS1 hom 0.0492105520234772 0.004369091890568 695 Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance inositol phosphate biosynthetic process inositol heptakisphosphate kinase activity|inositol hexakisphosphate kinase activity|inositol-1,3,4,5,6-pentakisphosphate kinase activity|inositol heptakisphosphate 5-kinase activity cytoplasm YDL119C_p YDL119C hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0492047223282757 0.004373738179524 696 Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria transport transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YBR107C IML3 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0491796162083256 0.00439379905011219 697 Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 establishment of meiotic sister chromatid cohesion|ascospore formation|protein localization to chromosome, centromeric region|meiotic sister chromatid segregation|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome outer kinetochore YFR031C-A RPL2A hom FT TRANSLATION|RIBOSOME 0.0491717191857958 0.0044177905073952 698 Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR127W ARO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0491630416665867 0.00452033018970658 699 Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids aromatic amino acid family biosynthetic process shikimate kinase activity|shikimate 3-dehydrogenase (NADP+) activity|3-phosphoshikimate 1-carboxyvinyltransferase activity|3-dehydroquinate dehydratase activity|3-dehydroquinate synthase activity cytoplasm YBR090C_p YBR090C hom FT NUCLEUS 0.04892895400002 0.00464150880318198 700 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YDL175C AIR2 hom FT RNA PROCESSING|NUCLEUS 0.0489218552375424 0.00460463503639388 701 RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication nuclear polyadenylation-dependent CUT catabolic process|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|tRNA modification polynucleotide adenylyltransferase activity|RNA binding|protein binding, bridging nucleolus|TRAMP complex YPL131W RPL5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0488669121084766 0.00471255895789122 702 Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YIL124W AYR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0488165294289854 0.00469957978253 703 NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones phosphatidic acid biosynthetic process acylglycerone-phosphate reductase activity mitochondrial outer membrane|mitochondrion|integral to membrane|endoplasmic reticulum|lipid particle|cytoplasm YGR160W_d YGR160W hom 0.0487584843749714 0.00474296330149607 704 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL091C UTP25 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0486894007959045 0.00480258000398166 705 Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) U3 snoRNA binding|rRNA binding small-subunit processome|nucleolus|nucleus YGR097W ASK10 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|RNA POL II, HOLOENZYME 0.0485540644525438 0.00492133888981299 706 Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication cellular protein catabolic process|cellular response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter|positive regulation of glycerol transport molecular_function DNA-directed RNA polymerase II, holoenzyme|cytoplasm YGL017W ATE1 hom FT PROTEOLYSIS 0.0485415808759583 0.00493242599603358 707 Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway cellular protein modification process|protein arginylation arginyltransferase activity cytoplasm YAR014C BUD14 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0485367908545443 0.00494957715891401 708 Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress regulation of cell shape during vegetative growth phase|regulation of protein localization|regulation of transcription, DNA-dependent|cytoskeleton organization|negative regulation of actin filament polymerization protein phosphatase type 1 regulator activity incipient cellular bud site|cellular bud neck|cellular bud tip YBR015C MNN2 hom FF|FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0485357204972216 0.00493763859024655 709 Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YLL006W MMM1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0485329249701237 0.00495302494297409 710 ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization|mitochondrial genome maintenance|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrion|integral to endoplasmic reticulum membrane|ERMES complex YOR258W HNT3 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0482757033284317 0.00517398702956925 711 DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress response to DNA damage stimulus DNA 5'-adenosine monophosphate hydrolase activity nucleus|cytoplasm YBR262C AIM5 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0482256872496503 0.00522060517642973 712 Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YMR222C FSH2 hom 0.0481802212982371 0.00526331108845505 713 Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm YGR078C PAC10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0481256434078021 0.00540444748290823 714 Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding tubulin complex assembly tubulin binding prefoldin complex|polysome|cytoplasm YKR049C FMP46 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0480986120329957 0.00534761145578439 715 Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process oxidoreductase activity mitochondrion YJL177W RPL17B hom FT TRANSLATION|RIBOSOME 0.0480914059318282 0.00534764593138372 716 Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJR117W STE24 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0480265626683092 0.00540998629865538 717 Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans CAAX-box protein processing|peptide pheromone maturation metalloendopeptidase activity mitochondrial outer membrane|integral to endoplasmic reticulum membrane|integral to membrane|nuclear inner membrane YNL308C KRI1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.048025356335449 0.00541115225660889 718 Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YDL117W CYK3 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0479840006565881 0.00545126119788975 719 SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants barrier septum assembly involved in cell cycle cytokinesis|cytokinesis molecular_function cellular bud neck contractile ring|cellular bud neck|cytoplasm YLR436C ECM30 hom FT CELL WALL ORG/BIOGENESIS 0.0477981903306677 0.00563480026595079 720 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization molecular_function cytoplasm YNR006W VPS27 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0477935413156322 0.00569694337401656 721 Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) protein retention in Golgi apparatus|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding|phosphatidylinositol-3-phosphate binding endosome|ESCRT-0 complex YER087C-B SBH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0477903514033365 0.00564266446125976 722 Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation ARF guanyl-nucleotide exchange factor activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex YDL062W_d YDL062W hom 0.0477736335436423 0.00565946927822223 723 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Unknown Unknown Unknown YKR041W YKR041W hom FT NUCLEUS|CYTOSKELETON 0.0477169383720625 0.00572402522681734 724 Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking biological_process molecular_function nucleus|mitotic spindle|cytoplasm YHL016C DUR3 hom FT PLASMA MEMBRANE 0.0477155024816329 0.00571825453490592 725 Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway urea transport|spermidine transport|putrescine transport|urea catabolic process urea transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YNL084C END3 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.047685855428794 0.00574844668098706 726 EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p bipolar cellular bud site selection|ascospore wall assembly|endocytosis|actin filament organization|cytokinesis|actin cortical patch assembly protein binding, bridging mating projection tip|actin cortical patch YCL035C GRX1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0476119662297587 0.00582432069694894 727 Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress protein glutathionylation|cellular response to oxidative stress disulfide oxidoreductase activity|glutathione transferase activity|glutathione peroxidase activity nucleus|cytoplasm YDR093W DNF2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0475914054600609 0.00584559366596512 728 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity integral to membrane|plasma membrane YOR180C DCI1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.047572082486905 0.00586564975168131 729 Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed fatty acid beta-oxidation dodecenoyl-CoA delta-isomerase activity peroxisomal matrix YKL100C_p YKL100C hom FT PROTEOLYSIS 0.0475529214830717 0.00588559892397855 730 Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene biological_process molecular_function integral to membrane YDL110C TMA17 hom FT NUCLEUS 0.0475335435381817 0.0059579470091206 731 Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion biological_process molecular_function ribosome|nucleus|cytoplasm YGL121C GPG1 hom FT SIGNALING 0.0475019665966539 0.00593894754125732 732 Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing signal transduction|invasive growth in response to glucose limitation signal transducer activity cellular_component YLR094C GIS3 hom FT SIGNALING|NUCLEUS 0.0474937685523745 0.00594757126988591 733 Protein of unknown function intracellular signal transduction molecular_function nucleus|cytoplasm YKL041W VPS24 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.047482252987195 0.00595970383178797 734 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YER148W SPT15 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0473939667667333 0.00606102380559957 735 TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability transposon integration|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly RNA polymerase III regulatory region DNA binding|RNA polymerase I transcription factor recruiting transcription factor activity|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding|DNA binding, bending|sequence-specific DNA binding|RNA polymerase II core promoter sequence-specific DNA binding|TFIIIB-type transcription factor activity|chromatin binding|RNA polymerase I transcription factor binding transcription factor TFIIIB complex|RNA polymerase I upstream activating factor complex|nucleus|RNA polymerase I core factor complex|transcription factor TFIID complex YJR066W TOR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS|PLASMA MEMBRANE 0.0473321044929214 0.00611995011546178 736 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication regulation of cell cycle|G1 phase of mitotic cell cycle|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell growth|response to DNA damage stimulus|mitochondria-nucleus signaling pathway|ribosome biogenesis|meiosis|signal transduction|TOR signaling cascade|fungal-type cell wall organization|negative regulation of autophagy protein binding|protein kinase activity TORC1 complex|fungal-type vacuole membrane|endosome membrane|extrinsic to internal side of plasma membrane|plasma membrane|nucleus|Golgi membrane|cytoplasm YIL127C_p RRT14 hom FT NUCLEUS 0.047294129123777 0.00621494972707786 737 Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleolus YOL164W BDS1 hom 0.0472402569205294 0.00625858496484052 738 Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources dodecyl sulfate metabolic process arylsulfatase activity|alkyl sulfatase activity cellular_component YGR037C ACB1 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.0472365895919569 0.00622389669918427 739 Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress long-chain fatty acid transport|chronological cell aging|fatty acid metabolic process long-chain fatty acid transporter activity|fatty-acyl-CoA binding extracellular region|nucleus|cytoplasm YMR031W-A_d YMR031W-A hom 0.0471946505824796 0.00630898931550624 740 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Unknown Unknown Unknown YOR034C-A_p YOR034C-A hom 0.0471665080958671 0.0063089730468126 741 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YMR312W ELP6 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0470931871469066 0.00640661108216405 742 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity protein urmylation|regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification ATPase activity|tRNA binding Elongator holoenzyme complex YPR067W ISA2 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0470915938812178 0.00638473043034421 743 Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations iron-sulfur cluster assembly|biotin biosynthetic process iron ion binding mitochondrion|mitochondrial intermembrane space YGL020C GET1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0470561960717107 0.00642455817887139 744 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance retrograde vesicle-mediated transport, Golgi to ER|protein insertion into ER membrane protein anchor mitochondrion|integral to membrane|endoplasmic reticulum|GET complex YGR025W_d YGR025W hom 0.0469176394581046 0.00660685443528881 745 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR179W OYE2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS|MITOCHONDRION 0.04684770798678 0.00666370449333701 746 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress apoptotic process NADPH dehydrogenase activity mitochondrion|nucleus|cytoplasm YLR322W_d VPS65 hom 0.0466207748672296 0.00712034334078056 747 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Unknown Unknown Unknown YOR235W_d IRC13 hom 0.0465634900829833 0.00700259860781492 748 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YLR148W PEP3 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.0465602879072592 0.0070489628374199 749 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|vacuole fusion, non-autophagic|late endosome to vacuole transport|vesicle docking involved in exocytosis|Golgi to endosome transport phosphatidylinositol binding|protein binding, bridging fungal-type vacuole membrane|HOPS complex|proteasome storage granule|CORVET complex|extrinsic to vacuolar membrane YJL193W_p YJL193W hom 0.0464697193768099 0.00716073624525985 750 Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect biological_process molecular_function integral to membrane YER084W_p YER084W hom 0.0464512990258552 0.0071491726414815 751 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YAR015W ADE1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0464233984792916 0.00718389492253342 752 N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolesuccinocarboxamide synthase activity nucleus|cytoplasm YJL127C-B_p YJL127C-B hom 0.0463091316436642 0.0073716533850039 753 Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii biological_process molecular_function cellular_component YJL161W_p FMP33 hom FT MITOCHONDRION 0.0460701702357095 0.00762771133537481 754 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YEL043W YEL043W hom FT ENDOPLASMIC RETICULUM 0.0460640523712388 0.00763576792132201 755 Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold biological_process molecular_function ribosome|endoplasmic reticulum YLR096W KIN2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.0460593296033878 0.00764199252507704 756 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p protein autophosphorylation|exocytosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity plasma membrane YFR012W-A_p YFR012W-A hom 0.0459867329325309 0.00774739476513792 757 Putative protein of unknown function; identified by homology biological_process molecular_function cellular_component YFR011C AIM13 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0459539012461578 0.00778213573062313 758 Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial crista junction|cytoplasm|mitochondrial intermembrane space YOR304C-A_p YOR304C-A hom FT SITE OF POLARIZED GROWTH 0.0459415765504892 0.00783553888975657 759 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck biological_process molecular_function cellular bud|mating projection tip|cellular bud neck|cytoplasm YML011C RAD33 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.045869351035744 0.00789618456476708 760 Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus nucleotide-excision repair molecular_function nucleus YBL086C_p YBL086C hom 0.045851805168044 0.00792003863955544 761 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YFL007W BLM10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0458436586983554 0.00793113589629898 762 Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 proteasome assembly|regulation of proteasomal protein catabolic process proteasome binding|peptidase activator activity proteasome core complex|proteasome complex|integral to membrane|nucleus YBL001C ECM15 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS 0.0458228809351119 0.00796884935430614 763 Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis fungal-type cell wall organization molecular_function nucleus|cytoplasm YPL178W CBC2 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.045820699944842 0.00799995016913219 764 Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif mRNA splicing, via spliceosome RNA cap binding commitment complex|nuclear cap binding complex YJR127C RSF2 hom FT NUCLEUS 0.045757695533875 0.00804908754831705 765 Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YAL025C MAK16 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0456895413910849 0.00814371618429344 766 Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|preribosome, large subunit precursor YOL018C TLG2 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0456734682480451 0.00832978463003306 767 Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) inositolphosphoceramide metabolic process|Golgi to vacuole transport|endocytosis|mannosyl-inositol phosphorylceramide metabolic process|protein exit from endoplasmic reticulum|vesicle fusion|CVT pathway SNAP receptor activity SNARE complex|trans-Golgi network|integral to endosome membrane YKL099C UTP11 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0456531920774083 0.00819459130935112 768 Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YGL013C PDR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RSC COMPLEX 0.0456230018498864 0.00823706200017998 769 Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Unknown|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YJR041C URB2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0455930178469845 0.00832771973358395 770 Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis rRNA metabolic process|ribosome biogenesis molecular_function nucleolus YGL061C DUO1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0455830771400132 0.0083031752392514 771 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|DASH complex|condensed nuclear chromosome kinetochore|spindle pole body|spindle YGL066W SGF73 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RNA LOCALIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0455503325520177 0.00834009930810421 772 SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay histone deubiquitination|mRNA export from nucleus|histone acetylation|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|protein complex assembly|chromatin modification histone acetyltransferase activity|enzyme activator activity|structural molecule activity DUBm complex|SLIK (SAGA-like) complex|SAGA complex YDR025W RPS11A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.045449118696645 0.00853472935704433 773 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YOR238W_p YOR238W hom 0.0452499040035972 0.00877841235223573 774 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YGL070C RPB9 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|NUCLEUS|RNA POL II, HOLOENZYME 0.0452207145736643 0.00891351868907489 775 RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription transcription initiation from RNA polymerase II promoter|maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter|transcription-coupled nucleotide-excision repair|response to DNA damage stimulus|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity DNA-directed RNA polymerase II, core complex YDR501W PLM2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0452123220398334 0.00886507295549688 776 Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication response to DNA damage stimulus chromatin binding nuclear chromatin YDR442W_d YDR442W hom 0.0451545746049219 0.00895236685021372 777 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YEL049W PAU2 hom 0.0451415530378631 0.00894147007740103 778 Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YPL183C RTT10 hom FT VESICLE-MEDIATED TRANSPORT 0.0450304993083145 0.00914252546152693 779 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endocytic recycling molecular_function endosome|cytoplasm YKL171W NNK1 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION 0.0450234619288329 0.00912226032124685 780 Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm proteolysis|protein phosphorylation protein kinase activity cytoplasm YHR066W SSF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0449775110917346 0.00923529981278054 781 Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication conjugation with cellular fusion|ribosomal large subunit assembly|regulation of cell size rRNA binding nucleolus YLR264C-A_p YLR264C-A hom 0.0448190168812671 0.00944297660362479 782 Putative protein of unknown function biological_process molecular_function cellular_component YMR105C PGM2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0447682249494692 0.00957791891630572 783 Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress glucose 6-phosphate metabolic process|cellular calcium ion homeostasis|galactose catabolic process|cellular cation homeostasis|glucose 1-phosphate metabolic process|trehalose biosynthetic process|UDP-glucose metabolic process|glycogen biosynthetic process phosphoglucomutase activity cytoplasm YOR082C_d YOR082C hom 0.0447603265312399 0.00953688371250681 784 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Unknown Unknown Unknown YOR016C ERP4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0446859708961943 0.00965705077705678 785 Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport ER to Golgi vesicle-mediated transport molecular_function integral to membrane YKL156W RPS27A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0445795027822201 0.00984244491726943 786 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasm YOL048C RRT8 hom 0.0444757261198826 0.0100041631580813 787 Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|lipid particle YAL012W CYS3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0444405626211522 0.0101869282685614 788 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress sulfur amino acid metabolic process|cysteine biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|cysteine metabolic process cystathionine gamma-lyase activity cytoplasm YDR236C FMN1 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0443872804818361 0.0102325057553565 789 Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane FMN biosynthetic process riboflavin kinase activity|FMN adenylyltransferase activity intracellular membrane-bounded organelle|mitochondrial inner membrane YCR008W SAT4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0443190282176835 0.0102700499164469 790 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p cellular cation homeostasis|protein phosphorylation|cellular protein localization|G1/S transition of mitotic cell cycle|protein dephosphorylation protein kinase activity cellular_component YDL165W CDC36 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0442856558532798 0.0103274820321998 791 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|nucleus|cytoplasm YDL035C GPR1 hom FT SIGNALING|PLASMA MEMBRANE 0.0442267702640494 0.0104295182371334 792 Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis replicative cell aging|G-protein coupled receptor signaling pathway|sucrose mediated signaling|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|invasive growth in response to glucose limitation|detection of glucose|detection of sucrose stimulus G-protein coupled receptor activity|glucose binding integral to membrane|plasma membrane YBR290W BSD2 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.0441549120893955 0.010555247116729 793 Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification metal ion transport|ubiquitin-dependent protein catabolic process|protein targeting to vacuole protein binding fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum YOL038C-A_p YOL038C-A hom 0.0441062909809465 0.0106410811472128 794 Putative protein of unknown function; identified by SAGE analysis biological_process molecular_function cellular_component YDR061W_p YDR061W hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0440967262723594 0.0106580392009718 795 Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance biological_process molecular_function mitochondrion YNL191W DUG3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0440055591334162 0.0108208864506382 796 Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process peptidase activity|omega peptidase activity|gamma-glutamyltransferase activity|carbon-nitrogen ligase activity, with glutamine as amido-N-donor cytoplasm YJR129C_p YJR129C hom 0.0440040580201676 0.0108235862135303 797 Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process S-adenosylmethionine-dependent methyltransferase activity cytoplasm YBR173C UMP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.043999619591302 0.0108315722607117 798 Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly proteasome assembly|response to DNA damage stimulus|ubiquitin-dependent protein catabolic process molecular_function nucleus|cytoplasm YNL001W DOM34 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.0439982085155986 0.010869598520124 799 Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication nuclear-transcribed mRNA catabolic process, no-go decay|ribosome disassembly|nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay|nonfunctional rRNA decay ribosome binding|endoribonuclease activity cytoplasm YLR418C CDC73 hom FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME|RNA POL II, HOLOENZYME 0.0439394427214957 0.0109403660970709 800 Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress transcription elongation from RNA polymerase I promoter|negative regulation of DNA recombination|mRNA 3'-end processing|recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex|positive regulation of transcription elongation from RNA polymerase II promoter|Unknown|regulation of transcription-coupled nucleotide-excision repair|transcription elongation from RNA polymerase II promoter chromatin binding|TFIIF-class binding transcription factor activity|RNA polymerase II core binding|RNA polymerase II transcription factor binding transcription factor activity nucleus|transcriptionally active chromatin|Cdc73/Paf1 complex YGL127C SOH1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0439387939051575 0.0109415443629743 801 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits DNA repair|meiotic gene conversion|meiotic sister chromatid segregation|transcription from RNA polymerase II promoter molecular_function core mediator complex|nucleus YLR082C SRL2 hom FT NUCLEUS 0.043916321613873 0.0109824242033756 802 Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation nucleobase-containing compound metabolic process molecular_function nucleus|cytoplasm YBR027C_d YBR027C hom 0.0437636496013916 0.0112637626235711 803 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR261C APL6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0437630765605837 0.0112648305424569 804 Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools vesicle-mediated transport|Golgi to vacuole transport molecular_function AP-3 adaptor complex YDR398W UTP5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0436719277363096 0.0114358437711865 805 Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|t-UTP complex|rDNA heterochromatin|nucleolus YNL059C ARP5 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0433031893957463 0.0121514079525886 806 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes nucleosome mobilization|chromatin remodeling ATP-dependent 3'-5' DNA helicase activity nucleus|Ino80 complex YKL059C MPE1 het FT RNA PROCESSING|NUCLEUS 0.0432770427883601 0.012203621748353 807 Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YLL061W MMP1 hom FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0432356076214443 0.012286773249219 808 High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p S-methylmethionine transport S-methylmethionine transmembrane transporter activity integral to membrane|plasma membrane YPR131C NAT3 hom FT MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0431735700635212 0.0124122073070026 809 Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met mitochondrion inheritance|N-terminal peptidyl-methionine acetylation|cytoskeleton organization peptide alpha-N-acetyltransferase activity NatB complex|cytoplasm YPR101W SNT309 hom FT RNA PROCESSING|NUCLEUS 0.0431724583586392 0.012414465370564 810 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YKL122C SRP21 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEUS 0.0430197004570279 0.0127282223580172 811 Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding nucleus|signal recognition particle, endoplasmic reticulum targeting YFR040W SAP155 hom FT CELL CYCLE 0.0428363240115821 0.0131141181440597 812 Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity cytoplasm YCL038C ATG22 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.0428316367860232 0.0131241160071889 813 Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation amino acid export from vacuole molecular_function integral to fungal-type vacuolar membrane|integral to membrane YMR178W YMR178W hom FT COFACTOR METABOLISM|NUCLEUS 0.0427798792128499 0.0132349637802856 814 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; YMR178W is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL213C SKI8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0426974222092878 0.013413269099807 815 Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|protein complex assembly|nuclear-transcribed mRNA catabolic process, non-stop decay|protein-DNA complex assembly|reciprocal meiotic recombination molecular_function Ski complex|nuclear chromosome YER172C BRR2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0426900215848617 0.0134293755371272 816 RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) ATP-dependent RNA helicase activity U4/U6 x U5 tri-snRNP complex|U5 snRNP YOR067C ALG8 hom FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0426409004667184 0.0135367145516771 817 Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p oligosaccharide-lipid intermediate biosynthetic process|protein N-linked glycosylation dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YLR346C_p YLR346C hom FT MITOCHONDRION 0.0424983950571823 0.0138666036764049 818 Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene biological_process molecular_function mitochondrion YGL042C_d YGL042C hom 0.0424971854624821 0.013897738090402 819 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Unknown Unknown Unknown YMR268C PRP24 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0424475724711506 0.0139665679956897 820 Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling assembly of spliceosomal tri-snRNP|spliceosomal complex assembly U6 snRNA binding|snRNA binding U6 snRNP YKL089W MIF2 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0424204727553152 0.0140277751912248 821 Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 chromosome segregation|mitotic spindle organization in nucleus centromeric DNA binding condensed nuclear chromosome, centromeric region|condensed nuclear chromosome inner kinetochore YLL046C RNP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS 0.0424007894865522 0.0141442990406373 822 Ribonucleoprotein that contains two RNA recognition motifs (RRM) ribosome biogenesis RNA binding cytoplasm YML074C FPR3 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0422874634104509 0.0143461804714137 823 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p meiotic recombination checkpoint peptidyl-prolyl cis-trans isomerase activity|macrolide binding nucleolus YIR001C SGN1 hom 0.0422854515258242 0.014336273124862 824 Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation mRNA metabolic process poly(A) RNA binding cytoplasm YPR103W PRE2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0422324417624951 0.0144590161153473 825 Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasome core complex assembly|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YDL063C SYO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0421951526237798 0.0146048326979743 826 Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus ribosomal large subunit biogenesis molecular_function nucleus|cytoplasm YKL057C NUP120 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0421118694322373 0.0147416541124154 827 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 double-strand break repair|protein export from nucleus|ribosomal large subunit export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|telomere tethering at nuclear periphery|nuclear pore distribution|mRNA export from nucleus in response to heat stress|mRNA export from nucleus|positive regulation of transcription, DNA-dependent|negative regulation of transcription from RNA polymerase II promoter|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore outer ring YLR304C ACO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0420935651818506 0.014784984621022 828 Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy propionate metabolic process|glutamate biosynthetic process|tricarboxylic acid cycle|mitochondrial genome maintenance|citrate metabolic process double-stranded DNA binding|aconitate hydratase activity|single-stranded DNA binding mitochondrial nucleoid|cytosol|mitochondrion|mitochondrial matrix YBR131W CCZ1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0420042667443602 0.0149979879143746 829 Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway autophagy|autophagic vacuole fusion|vacuolar transport|vesicle docking|protein targeting to vacuole guanyl-nucleotide exchange factor activity Mon1-Ccz1 complex|membrane YKR035C_d OPI8 hom 0.041976460918523 0.0151404671037026 830 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Unknown Unknown Unknown YLR441C RPS1A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0419573888999183 0.0151108833780193 831 Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YMR033W ARP9 het FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0418680823221586 0.0153433132814233 832 Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation nucleosome positioning|ATP-dependent chromatin remodeling|nucleosome mobilization|chromatin organization|nucleosome disassembly|transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA translocase activity RSC complex|SWI/SNF complex|nucleus YMR012W CLU1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION 0.0418339853315709 0.01541165920636 833 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation|mitochondrion organization molecular_function eukaryotic translation initiation factor 3 complex|cytoplasm YLR114C AVL9 hom FT VESICLE-MEDIATED TRANSPORT 0.0417095288569654 0.0157203235854408 834 Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress post-Golgi vesicle-mediated transport molecular_function cytoplasm YEL008W_d YEL008W hom 0.0416802992499519 0.0158561717723314 835 Hypothetical protein predicted to be involved in metabolism Unknown Unknown Unknown YBR044C TCM62 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0416579124290337 0.0158499237414602 836 Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone mitochondrial respiratory chain complex II assembly unfolded protein binding mitochondrion|mitochondrial inner membrane YGR216C GPI1 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0415498179228504 0.0161243773766068 837 Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YKL112W ABF1 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS 0.0415239797822811 0.0161905958775395 838 DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair negative regulation of transcription from RNA polymerase II promoter|global genome nucleotide-excision repair|chromatin remodeling|DNA-dependent DNA replication|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific DNA binding, bending nucleotide-excision repair factor 4 complex|nucleus YBR247C ENP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0415043427980378 0.0162410815852087 839 Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing snoRNA binding preribosome, small subunit precursor|nucleolus|nucleus|cytoplasm|90S preribosome YDR486C VPS60 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0414596638636966 0.0163564644278271 840 Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p late endosome to vacuole transport via multivesicular body sorting pathway|filamentous growth molecular_function fungal-type vacuole membrane|cytoplasm YDR422C SIP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION 0.041437641922932 0.0164136002376824 841 Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions signal transduction|protein phosphorylation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|fungal-type vacuole YOL088C MPD2 hom FT ENDOPLASMIC RETICULUM 0.0412842524690592 0.0168164524417984 842 Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p protein folding protein disulfide isomerase activity|protein disulfide oxidoreductase activity endoplasmic reticulum YCR068W ATG15 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0412500181184796 0.0169075386529652 843 Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway membrane disassembly|autophagy|piecemeal microautophagy of nucleus|vacuolar protein processing|multivesicular body membrane disassembly lipase activity integral to membrane|vacuolar lumen YKL195W MIA40 het FF|FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0412370535291014 0.0170244901358548 844 Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial outer membrane translocase complex|mitochondrial intermembrane space YDR482C CWC21 hom FT RNA PROCESSING|NUCLEUS 0.041184663536479 0.0170991622686344 845 Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YER060W FCY21 hom 0.0411492007024351 0.0171782990999655 846 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YGR258C RAD2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0411117326830253 0.0172798899701711 847 Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein nucleotide-excision repair, DNA incision, 3'-to lesion|transcription from RNA polymerase II promoter single-stranded DNA specific endodeoxyribonuclease activity nucleotide-excision repair factor 3 complex YGR086C PIL1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.0411080340804649 0.0173066259984986 848 Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress eisosome assembly|endocytosis|negative regulation of protein kinase activity|response to heat|protein localization lipid binding mitochondrial outer membrane|eisosome|mitochondrion|plasma membrane|cytoplasm YGL144C ROG1 hom FT LIPID METABOLISM 0.0411056365886599 0.0172964686579016 849 Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication cellular lipid metabolic process lipase activity cellular_component YJL035C TAD2 het FT RNA PROCESSING|NUCLEUS 0.0410494574204893 0.0174499087454239 850 Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YDL173W PAR32 hom 0.0409581828472308 0.0177017478144885 851 Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene biological_process molecular_function cytoplasm YGL226C-A OST5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0409022963406309 0.0178575130099057 852 Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YOR010C TIR2 hom 0.0408673464392115 0.0179555332374704 853 Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis response to stress molecular_function fungal-type cell wall YPL100W ATG21 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0408090717836991 0.0181200177167836 854 Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein piecemeal microautophagy of nucleus|vesicle organization|mitochondrion degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway|protein lipidation phosphatidylinositol binding endosome|cytosol|fungal-type vacuole membrane YER139C RTR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0407734031769015 0.0182560044336277 855 CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication dephosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase II promoter CTD phosphatase activity nucleus|cytoplasm YJR076C CDC11 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0407011772291818 0.0184979942701956 856 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells maintenance of cell polarity|cell cycle cytokinesis|positive regulation of protein kinase activity structural molecule activity|GTP binding|1-phosphatidylinositol binding spindle microtubule|septin complex|prospore membrane|ascospore wall|mating projection base|meiotic spindle|cellular bud neck septin ring|septin filament array|cytoplasmic microtubule|mating projection tip YLR136C TIS11 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 0.0406817667838009 0.018483939949505 857 mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress cellular iron ion homeostasis|nuclear-transcribed mRNA catabolic process mRNA binding nucleus|cytoplasm YER026C CHO1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0405495091516403 0.0188687760489019 858 Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline phosphatidylserine biosynthetic process CDP-diacylglycerol-serine O-phosphatidyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YDL137W ARF2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0405356011302233 0.0189096490138832 859 ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport GTPase activity cytosol|Golgi-associated vesicle YOR194C TOA1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0404917489151528 0.0190390292605095 860 TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription factor TFIIA complex YAL038W CDC19 het FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.040459493914553 0.0192606277280668 861 Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication pyruvate metabolic process|glycolysis pyruvate kinase activity cytosol|plasma membrane YFR027W ECO1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.040434265311888 0.0192097984222703 862 Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress double-strand break repair|regulation of DNA replication|mitotic chromosome condensation|internal peptidyl-lysine acetylation|establishment of mitotic sister chromatid cohesion|DNA repair|chromosome organization|DNA replication|regulation of mitosis|telomere organization|tRNA gene clustering acetyltransferase activity|chromatin binding nuclear chromatin|nuclear replication fork YOR224C RPB8 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0403995913374051 0.0194220675272649 863 RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YGR271C-A EFG1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0403473953754204 0.0194704059671572 864 Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus G1 phase of mitotic cell cycle|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YDR242W AMD2 hom 0.0402998240536804 0.0198531693942496 865 Putative amidase biological_process amidase activity cellular_component YDR537C_d YDR537C hom 0.0402290637360685 0.0198303621908214 866 Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Unknown Unknown Unknown YGL210W YPT32 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0401763809321857 0.0200480598849407 867 Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication early endosome to Golgi transport|vesicle-mediated transport|exocytosis GTPase activity endosome|mitochondrial outer membrane|Golgi apparatus YHR003C YHR003C hom FT MITOCHONDRION 0.0401642367343558 0.0200300129610727 868 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion YER007C-A TMA20 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION 0.0401338636298293 0.0201241561926029 869 Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress ribosome biogenesis RNA binding ribosome|cytoplasm YER166W DNF1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE 0.0401310644984596 0.0201328516585936 870 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YOR014W RTS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.040121349522261 0.020181677953586 871 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle spindle orientation checkpoint|cellular protein localization|meiotic sister chromatid cohesion, centromeric|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|spindle pole body|condensed nuclear chromosome, centromeric region|cellular bud neck|cytoplasm YMR044W IOC4 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0400024386272116 0.0206304840129491 872 Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YMR052C-A_d YMR052C-A hom 0.0399353666722917 0.0207489656901921 873 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFL041W FET5 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0398813572932659 0.0209792715344548 874 Multicopper oxidase, integral membrane protein with similarity to Fet3p; may have a role in iron transport iron ion transport ferroxidase activity fungal-type vacuole membrane|integral to membrane YOL041C NOP12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0398399182419332 0.0210553809859704 875 Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA binding nucleolus|preribosome YML026C RPS18B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|MITOCHONDRION|RIBOSOME 0.0398009264467844 0.0212589277312158 876 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit|mitochondrion YLR258W GSY2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0397979012520647 0.0211915113668865 877 Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress glycogen biosynthetic process glycogen (starch) synthase activity cytoplasm YAL041W CDC24 het FT CELL CYCLE|SIGNALING|NUCLEUS|SITE OF POLARIZED GROWTH 0.0397624742074618 0.021306885899115 878 Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of exit from mitosis|establishment of cell polarity|small GTPase mediated signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|nucleus|cellular bud neck|mating projection tip|cellular bud tip|cytoplasm YPL083C SEN54 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0397530779638271 0.0213375780541286 879 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YCR024C-A PMP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0397281945738114 0.0214190438447669 880 Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication cation transport enzyme regulator activity plasma membrane|membrane YCL001W RER1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0397108505182246 0.0214759865767494 881 Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER protein retention in ER lumen|retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport molecular_function COPI-coated vesicle|integral to membrane|ER to Golgi transport vesicle YER116C SLX8 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0396926368960204 0.021535925985277 882 Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress protein sumoylation|telomere maintenance|response to DNA damage stimulus|protein ubiquitination ubiquitin-protein ligase activity ubiquitin ligase complex|nucleus YOR035C SHE4 hom FF|FT CYTOSKELETON ORGANIZATION|RNA LOCALIZATION 0.0396511571033389 0.0218500224534099 883 Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization mating type switching|intracellular mRNA localization|actin cytoskeleton organization myosin binding cytoplasm YOR289W_p YOR289W hom FT NUCLEUS 0.0396207195841638 0.0219714800596819 884 Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YPR040W TIP41 hom FT SIGNALING|NUCLEUS 0.0395801560514716 0.0219093258483861 885 Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress signal transduction molecular_function nucleus|cytoplasm YNL271C BNI1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0394614090493089 0.0223496469683684 886 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 barbed-end actin filament capping|actin nucleation|actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assembly|establishment of mitotic spindle orientation|formin-nucleated actin cable assembly profilin binding incipient cellular bud site|actin filament|polarisome|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YPL069C BTS1 hom FF|FT PROTEIN LOCALIZATION|LIPID METABOLISM|MITOCHONDRION 0.0394348184056249 0.022561121226397 887 Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic terpenoid biosynthetic process farnesyltranstransferase activity mitochondrion YPL220W RPL1A hom FT TRANSLATION|RIBOSOME 0.0393791670640197 0.0225905763718923 888 Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YKL126W YPK1 hom FF|FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0392870422310766 0.022970123205888 889 Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication eisosome assembly|protein autophosphorylation|negative regulation of phospholipid translocation|protein phosphorylation protein serine/threonine kinase activity cytosol|plasma membrane|cellular bud neck YOR300W_d YOR300W hom 0.0391102001723335 0.0235309810619654 890 Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Unknown Unknown Unknown YDL048C STP4 hom FT NUCLEUS|MITOCHONDRION 0.0391084451080183 0.0235372272577372 891 Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress biological_process sequence-specific DNA binding mitochondrion|nucleus|cytoplasm YDL074C BRE1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0390998071056611 0.0235679904866934 892 E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control mitotic cell cycle G1/S transition DNA damage checkpoint|intra-S DNA damage checkpoint|meiotic DNA double-strand break formation|histone monoubiquitination|double-strand break repair via homologous recombination|transcription from RNA polymerase II promoter|chromatin silencing at telomere ubiquitin-protein ligase activity nuclear chromatin YDL061C RPS29B hom FT TRANSLATION|RIBOSOME 0.0390906212821677 0.0236007429736315 893 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YDL236W PHO13 hom FT NUCLEUS 0.0390169854411844 0.0238647246782882 894 Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity protein dephosphorylation 4-nitrophenylphosphatase activity|phosphoprotein phosphatase activity|alkaline phosphatase activity nucleus|cytoplasm YLR016C PML1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.0390013185261322 0.0239422217213707 895 Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p mRNA export from nucleus|mRNA splicing, via spliceosome|maintenance of RNA location molecular_function RES complex|nucleus|cytoplasm YER147C SCC4 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0389828883162314 0.0239878262355122 896 Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX double-strand break repair|establishment of protein localization to chromatin|rDNA condensation|mitotic chromosome condensation|mitotic sister chromatid cohesion molecular_function nuclear chromatin|SMC loading complex YDL082W RPL13A hom FT TRANSLATION|RIBOSOME 0.0389584860803678 0.0240762637060997 897 Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YHR010W RPL27A hom FT TRANSLATION|RIBOSOME 0.0389038454684154 0.024275315097876 898 Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YNL322C KRE1 hom FT CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.038838119790197 0.0245166362415258 899 Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall|integral to membrane YGR207C CIR1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0387406578824884 0.0248783054824004 900 Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion YJR016C ILV3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0386925352499003 0.0250585795843534 901 Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids branched-chain amino acid biosynthetic process dihydroxy-acid dehydratase activity mitochondrion YKL220C FRE2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.0386657912625138 0.0251592541570176 902 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YLL045C RPL8B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0386645448806784 0.0251639545366616 903 Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YMR170C ALD2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0386160154513408 0.0253475613687786 904 Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular aldehyde metabolic process|beta-alanine biosynthetic process|polyamine catabolic process aldehyde dehydrogenase (NAD) activity cytoplasm YDR083W RRP8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0386106656806073 0.0253678724952004 905 Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 rRNA processing methyltransferase activity nucleolus YBR110W ALG1 het FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0385726861397527 0.0255124720912591 906 Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog oligosaccharide-lipid intermediate biosynthetic process|protein N-linked glycosylation beta-1,4-mannosyltransferase activity integral to membrane|endoplasmic reticulum YPL226W NEW1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0385479487777331 0.0256070375648549 907 ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction ribosomal small subunit biogenesis|poly(A)+ mRNA export from nucleus ATPase activity|ATP binding mitochondrion|polysome|cytoplasm YKL066W_d YKL066W hom 0.0384672721271517 0.0259175556481033 908 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 Unknown Unknown Unknown YLR065C ENV10 hom FF|FT VACUOLAR PROTEIN PROCESSING 0.0384651825502103 0.0260368807632503 909 Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene vacuolar protein processing molecular_function integral to membrane YGR031W IMO32 hom FT MITOCHONDRION 0.0384323525140513 0.0260529643826527 910 Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 biological_process molecular_function mitochondrion YOR023C AHC1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0384260971050442 0.0260772856052704 911 Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex YIL167W SDL1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0383595041464648 0.0263374195155244 912 Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase serine family amino acid metabolic process L-serine ammonia-lyase activity cellular_component YLR062C_d BUD28 hom 0.0383551031031715 0.0263546901152803 913 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YLR189C ATG26 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM 0.0383476226023358 0.0263840675828035 914 UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy sterol metabolic process|ascospore-type prospore membrane assembly sterol 3-beta-glucosyltransferase activity cytoplasm YDR176W NGG1 hom FF|FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0382900958407457 0.0266109335435537 915 Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex histone acetylation|chromatin modification histone acetyltransferase activity|transcription cofactor activity SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YGR085C RPL11B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0382715254576235 0.0268435272633088 916 Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YBL019W APN2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0382704800271707 0.0266886757401253 917 Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII base-excision repair DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity nucleus YLR228C ECM22 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0382206595608049 0.026955384910125 918 Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|membrane|perinuclear region of cytoplasm YBL068W PRS4 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0382004453571063 0.0269678417644658 919 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YBR259W YBR259W hom 0.0381999791620697 0.0269697084804943 920 Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component YDL076C RXT3 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0381589767436416 0.0271343251672308 921 Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase I promoter molecular_function Rpd3L-Expanded complex|nucleus|Rpd3L complex YJR033C RAV1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM 0.0381334929752906 0.0272370737102235 922 Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate early endosome to late endosome transport|vacuolar acidification|regulation of protein complex assembly molecular_function RAVE complex|cytoplasm|extrinsic to membrane YDL211C_p YDL211C hom 0.0379203832329063 0.0281800209557302 923 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YDR273W DON1 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION 0.0379064387732138 0.0282380376390998 924 Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II meiosis|ascospore wall assembly molecular_function prospore membrane YNL139C THO2 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS 0.0378256589910553 0.0285049889519864 925 Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|transcription elongation from RNA polymerase II promoter DNA binding|nucleic acid binding THO complex part of transcription export complex|nucleus|nucleoplasmic THO complex YPL264C_p YPL264C hom 0.0378230186899677 0.028516080063368 926 Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene biological_process molecular_function integral to membrane YLR282C_d YLR282C hom 0.0378163312946917 0.0286154206381445 927 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YDR484W VPS52 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS|CYTOSKELETON 0.0378015552681413 0.0286063793956012 928 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin actin filament-based process|Golgi to vacuole transport|retrograde transport, endosome to Golgi protein binding Golgi apparatus|GARP complex YOR061W CKA2 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0377422702445444 0.0288570790248078 929 Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching regulation of transcription from RNA polymerase I promoter|chronological cell aging|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase activity protein kinase CK2 complex|UTP-C complex YDR475C JIP4 hom 0.0377300324851916 0.0289090638207215 930 Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence biological_process molecular_function cellular_component YLR097C HRT3 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.0376476307361293 0.0292612007830405 931 Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YOR103C OST2 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0374503899133945 0.0301191081319658 932 Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YLR413W_p YLR413W hom FT PLASMA MEMBRANE 0.0374341762715501 0.0303140121899453 933 Putative protein of unknown function; YLR413W is not an essential gene biological_process molecular_function integral to membrane YCL001W-A_p YCL001W-A hom 0.0374317688828926 0.0302012061832482 934 Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III biological_process molecular_function cellular_component YER067C-A_d YER067C-A hom 0.0374245028652291 0.030233293340561 935 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Unknown Unknown Unknown YOL086W-A MHF1 hom FT RESPONSE TO DNA DAMAGE 0.0373350714744856 0.0306306317226998 936 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 response to DNA damage stimulus molecular_function cellular_component YIL022W TIM44 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0373288959415 0.0306582339485508 937 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) protein import into mitochondrial matrix protein binding, bridging|chaperone binding mitochondrion|integral to membrane|presequence translocase-associated import motor YEL026W SNU13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0373106984587549 0.0307646398247066 938 RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mRNA splicing, via spliceosome RNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|U4/U6 x U5 tri-snRNP complex YOR223W YOR223W hom FT ENDOPLASMIC RETICULUM 0.0373095520641115 0.0307448317635241 939 Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 biological_process molecular_function fungal-type vacuole lumen|endoplasmic reticulum YAL061W_p BDH2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0372964178705243 0.0308037499332759 940 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor nucleus|cytoplasm YIL053W RHR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.03727533167083 0.0308985423531704 941 Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YOR270C VPH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0372504275689023 0.031035914489451 942 Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress polyphosphate metabolic process|proton transport|protein complex assembly|vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YNL099C OCA1 hom FF|FT RESPONSE TO OXIDATIVE STRESS 0.0371857106478464 0.0313042289570263 943 Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA cellular response to oxidative stress protein tyrosine phosphatase activity cytoplasm YDR131C_p YDR131C hom FT PROTEOLYSIS 0.037183706044945 0.031414517588777 944 F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YPL175W SPT14 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0371075465662062 0.0319685547989862 945 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|endoplasmic reticulum YDR411C DFM1 hom FT SIGNALING|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0370159174891056 0.0321110401232933 946 Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p endoplasmic reticulum unfolded protein response|ER-associated protein catabolic process molecular_function Doa10p ubiquitin ligase complex|integral to membrane|endoplasmic reticulum|Hrd1p ubiquitin ligase ERAD-L complex YDR200C VPS64 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0369443589087003 0.0324195392165505 947 Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance re-entry into mitotic cell cycle after pheromone arrest|protein targeting to vacuole molecular_function endoplasmic reticulum membrane|cytoplasm YKL079W SMY1 hom FT VESICLE-MEDIATED TRANSPORT|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0369397388936387 0.0324412164612626 948 Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins exocytosis motor activity incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip YGL146C_p RRT6 hom 0.0369065266965943 0.0325974146622516 949 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins biological_process molecular_function membrane YJL156C SSY5 het FT PROTEOLYSIS|PLASMA MEMBRANE 0.0368110373698428 0.0330762850095047 950 Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p protein processing|response to amino acid stimulus serine-type endopeptidase activity extrinsic to plasma membrane YLL044W_d YLL044W hom 0.0367588113215155 0.0332999487603207 951 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Unknown Unknown Unknown YOL127W RPL25 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.03674873284927 0.0333483503413101 952 Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YER159C BUR6 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0367264259199985 0.0334556927421721 953 Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity|transcription corepressor activity nucleus|negative cofactor 2 complex YIL141W_d YIL141W hom 0.0366810683306434 0.033674867825303 954 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YEL046C GLY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0366063359562769 0.0341188540795916 955 Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis threonine catabolic process|glycine biosynthetic process threonine aldolase activity|L-allo-threonine aldolase activity cytosol YOL058W ARG1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0365697706085309 0.0342178833828594 956 Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate argininosuccinate metabolic process|arginine biosynthetic process|citrulline metabolic process argininosuccinate synthase activity cytosol YKR025W RPC37 het FT NUCLEUS 0.0365464533862159 0.0343594507860585 957 RNA polymerase III subunit C37 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YGR185C TYS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|NUCLEUS 0.0365081386407765 0.0345217877264065 958 Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress tyrosyl-tRNA aminoacylation tyrosine-tRNA ligase activity nucleus|cytoplasm YGL088W_d YGL088W hom 0.0365003484932671 0.0345603641572108 959 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Unknown Unknown Unknown YGR223C HSV2 hom FT MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS 0.0364984323508102 0.0345698584395292 960 Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization piecemeal microautophagy of nucleus phosphatidylinositol binding endosome|fungal-type vacuole|extrinsic to membrane|cytoplasm YDR108W TRS85 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|GOLGI APPARATUS 0.0364712914729835 0.0347045779623897 961 Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role meiosis|vesicle organization|piecemeal microautophagy of nucleus|ER to Golgi vesicle-mediated transport|peroxisome degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway molecular_function TRAPP complex|pre-autophagosomal structure YHR073W OSH3 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM 0.0364191904507985 0.0349644487514156 962 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability karyogamy involved in conjugation with cellular fusion|endocytosis|exocytosis|positive regulation of phosphatase activity|maintenance of cell polarity|pseudohyphal growth|sterol transport|invasive growth in response to glucose limitation lipid binding|oxysterol binding|1-phosphatidylinositol binding|sterol transporter activity cortical endoplasmic reticulum|cytoplasm YNL272C SEC2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SITE OF POLARIZED GROWTH 0.0363987427704502 0.0350668910252754 963 Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles autophagy|exocytosis guanyl-nucleotide exchange factor activity|phosphatidylinositol-4-phosphate binding cytosol|transport vesicle|mating projection tip|cellular bud neck|cellular bud tip YCR093W CDC39 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0363776666051295 0.0354466959320632 964 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex|nucleus|cytoplasm YGL219C MDM34 hom FT MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0363094943804024 0.0355170265257226 965 Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization|phospholipid transport molecular_function mitochondrial outer membrane|ERMES complex|mitochondrion|cytoplasm YDR269C_d YDR269C hom 0.0362671932372059 0.035732093140137 966 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR118W RPS23A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0361817369855656 0.0361699567277274 967 Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit YMR029C FAR8 hom FT CELL CYCLE|SIGNALING|ENDOPLASMIC RETICULUM 0.0361531463406754 0.036317467439672 968 Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YOR293C-A_p YOR293C-A hom 0.0361290976475212 0.0364419409284197 969 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YLR116W MSL5 het FT RNA PROCESSING|NUCLEUS 0.0360096741104342 0.037065460546675 970 Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA mRNA splicing, via spliceosome pre-mRNA branch point binding commitment complex YDR344C_d YDR344C hom 0.0359759162733695 0.0372433503763291 971 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL065W_d YBL065W hom 0.0359710563681335 0.0382458635973371 972 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Unknown Unknown Unknown YML081C-A ATP18 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0358918860825536 0.0376893121231012 973 Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YHR015W MIP6 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0358591233995918 0.0378644152911708 974 Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication mRNA export from nucleus RNA binding nuclear pore YJR118C ILM1 hom FF|FT MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0358377598654507 0.0379789668321813 975 Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth mitochondrial genome maintenance molecular_function integral to membrane|endoplasmic reticulum YLR207W HRD3 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0358239614804063 0.0380531104315855 976 Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events ER-associated protein catabolic process ubiquitin-protein ligase activity Hrd1p ubiquitin ligase ERAD-M complex|luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YBL071C-B_p YBL071C-B hom 0.0357755533810808 0.0383430694743676 977 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPL010W RET3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0356985382447001 0.0387327135260705 978 Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER molecular_function COPI vesicle coat YEL004W YEA4 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0356821410333881 0.0389097482515542 979 Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER fungal-type cell wall chitin biosynthetic process|UDP-N-acetylglucosamine transport|UDP-glucose transport|transmembrane transport UDP-N-acetylglucosamine transmembrane transporter activity integral to membrane|endoplasmic reticulum YLR269C_d YLR269C hom 0.0356744807783267 0.0388642398205396 980 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR032W GSC2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE|CYTOSKELETON 0.0355443662885683 0.0395821885699087 981 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) ascospore wall assembly|(1->3)-beta-D-glucan biosynthetic process 1,3-beta-D-glucan synthase activity 1,3-beta-D-glucan synthase complex|integral to membrane|actin cap|prospore membrane YKL134C OCT1 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0354672963933583 0.0400127296730026 982 Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis cellular iron ion homeostasis|protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion YDR203W_d YDR203W hom FF 0.03540212727692 0.0404396487674532 983 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL119C DBP1 hom FT TRANSLATION 0.0353667336721234 0.0405804759733079 984 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress translational initiation RNA helicase activity cytoplasm YNL044W YIP3 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.035337090829274 0.0408996020931717 985 Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus|endoplasmic reticulum|ER to Golgi transport vesicle YMR182W-A_p YMR182W-A hom 0.0351041630035677 0.0420951367262196 986 Putative protein of unknown function biological_process molecular_function cellular_component YER019C-A SBH2 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.035101346416443 0.042265328875801 987 Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p SRP-dependent cotranslational protein targeting to membrane ARF guanyl-nucleotide exchange factor activity Ssh1 translocon complex YPR132W RPS23B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0350747936384446 0.0422674922160444 988 Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit YMR147W_p YMR147W hom 0.0350437820186869 0.0424501327072386 989 Putative protein of unknown function biological_process molecular_function cellular_component YJL201W ECM25 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS 0.035033274268017 0.0425121684207236 990 Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p fungal-type cell wall organization|filamentous growth molecular_function cytoplasm YOR279C RFM1 hom FT GENE SILENCING|NUCLEUS 0.0350300668894028 0.0425311194194335 991 DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance chromatin silencing|chromatin silencing at silent mating-type cassette unfolded protein binding histone deacetylase complex|nucleus YDL184C RPL41A hom FT TRANSLATION|RIBOSOME 0.0349756618279915 0.0428536646784725 992 Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YCL076W_d YCL076W hom 0.0349734210884868 0.042866993301161 993 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR221W-A_p YBR221W-A hom 0.0348808993685681 0.0434517052356431 994 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YBR167C POP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0348125005574957 0.0438334008909804 995 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YLR366W_d YLR366W hom 0.0347563711495585 0.0442064252841322 996 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Unknown Unknown Unknown YDR259C YAP6 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0347507063107059 0.0443044066601173 997 Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding transcription factor recruiting transcription factor activity|sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding nucleus YIR004W DJP1 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0347274325726992 0.0443516637234734 998 Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ protein import into peroxisome matrix chaperone binding cytosol YJL122W ALB1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0347045354956755 0.0444920400467736 999 Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p ribosomal large subunit biogenesis molecular_function ribosome|nucleus|cytoplasm YOR055W_d YOR055W hom 0.0346443233836217 0.0448629749718454 1000 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR271W EFM2 hom 0.0344273538816971 0.0462213018628204 1001 S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis peptidyl-lysine methylation S-adenosylmethionine-dependent methyltransferase activity|protein-lysine N-methyltransferase activity cytoplasm YDR122W KIN1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.0343583646386302 0.0466603932282831 1002 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p protein autophosphorylation|exocytosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity internal side of plasma membrane|plasma membrane YNL136W EAF7 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0343462410926812 0.0467379165361788 1003 Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter molecular_function histone acetyltransferase complex|nucleus|NuA4 histone acetyltransferase complex YDR487C RIB3 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0343285382237557 0.0468513110790817 1004 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration riboflavin biosynthetic process|aerobic respiration 3,4-dihydroxy-2-butanone-4-phosphate synthase activity cytosol|mitochondrial intermembrane space YPR010C RPA135 het FT NUCLEUS 0.0343020628673355 0.0470213289244902 1005 RNA polymerase I second largest subunit A135 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex YIL126W STH1 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|RSC COMPLEX 0.0342370317950077 0.0474742443185825 1006 ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p double-strand break repair|ATP-dependent chromatin remodeling|nucleosome positioning|chromosome segregation|chromatin remodeling at centromere|nucleosome disassembly|regulation of transcription, DNA-dependent|G2/M transition of mitotic cell cycle|meiosis|cytoskeleton organization|transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|DNA translocase activity|helicase activity|DNA-dependent ATPase activity RSC complex|nucleus YML094W GIM5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0342136634132056 0.0475927696809533 1007 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YDL151C_d BUD30 hom 0.0341615436827025 0.0481996079971411 1008 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YLR283W_p YLR283W hom FT MITOCHONDRION 0.0341392414207017 0.0482454996364403 1009 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene biological_process molecular_function mitochondrion YDR008C_d YDR008C hom FF 0.0341298259515727 0.048609986983474 1010 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL226W_d YNL226W hom 0.0341294898557853 0.0481423056690816 1011 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Unknown Unknown Unknown YDR425W SNX41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT 0.0341121131319118 0.0482564125404721 1012 Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p retrograde transport, endosome to Golgi phosphatidylinositol binding|phosphatidylinositol-3-phosphate binding endosome YHR064C SSZ1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION 0.0340204897946701 0.0488618329875861 1013 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP cytoplasmic translation|regulation of translational fidelity|rRNA processing unfolded protein binding polysome|cytoplasm YBL071C_d YBL071C hom 0.0339856692432256 0.0490935823747669 1014 Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Unknown Unknown Unknown YBR077C SLM4 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|SIGNALING 0.0339819631411582 0.0494236190429796 1015 Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 signal transduction|microautophagy phosphatidylinositol-3,4-bisphosphate binding fungal-type vacuole membrane|EGO complex|late endosome membrane|cytoplasm YHR019C DED81 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0339329821551894 0.0495479665937777 1016 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity cytosol YAL060W BDH1 hom FT OXIDATION-REDUCTION PROCESS 0.0339111716890495 0.0495925011823373 1017 NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source alcohol metabolic process|butanediol biosynthetic process (R,R)-butanediol dehydrogenase activity cytoplasm YBL066C SEF1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0338950077975191 0.0497013120363412 1018 Putative transcription factor, has homolog in Kluyveromyces lactis biological_process molecular_function cellular_component YEL062W NPR2 hom FT SIGNALING|RESPONSE TO NUTRIENT LEVELS 0.0337856872208658 0.050545733773963 1019 Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline proline transport|urea transport|regulation of TOR signaling cascade|cellular response to amino acid starvation|response to drug|cellular response to nitrogen starvation molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YDR255C RMD5 hom FT KETONE METABOLISM|CELL CYCLE|CARBOHYDRATE METABOLISM|PROTEOLYSIS 0.0337543238526121 0.0506568353008826 1020 Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity cytosol|GID complex YKL210W UBA1 het FT NUCLEUS 0.0337070850039686 0.0511197203894065 1021 Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress protein ubiquitination ubiquitin activating enzyme activity nucleus|cytoplasm YMR152W YIM1 hom FT RESPONSE TO DNA DAMAGE|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0336892690686423 0.0511038606578041 1022 Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress response to DNA damage stimulus molecular_function mitochondrion|lipid particle|cytoplasm YDR088C SLU7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0336860680215803 0.0512300434565026 1023 RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity spliceosomal complex YLR003C CMS1 hom FT NUCLEUS 0.0336765634845908 0.0511915516276204 1024 Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process molecular_function nucleus|90S preribosome YMR242W-A_p YMR242W-A hom 0.0336643584596264 0.051275906403752 1025 Putative protein of unknown function biological_process molecular_function cellular_component YIL043C CBR1 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.033574674885131 0.0518993268410416 1026 Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia biological_process cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion YDR457W TOM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.033537318927772 0.0521608640171853 1027 E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mitosis|nucleocytoplasmic transport|nucleus organization|cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of cell size|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|nucleolus|nucleus YGL071W AFT1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION|NUCLEUS 0.0334077285624713 0.0531832340622635 1028 Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation|meiotic chromosome segregation|positive regulation of iron ion transport|establishment of mitotic sister chromatid cohesion|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|cytoplasm YFR014C CMK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.0333978713062642 0.0533247294838104 1029 Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YGR277C CAB4 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0333890441199462 0.0532453956695263 1030 Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved coenzyme A biosynthetic process pantetheine-phosphate adenylyltransferase activity nucleus|cytoplasm YJL188C_d BUD19 hom 0.0333841786686869 0.0532445760063247 1031 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YJL171C YJL171C hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0333417024844259 0.0535484718596138 1032 GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion|fungal-type cell wall|plasma membrane YJL072C PSF2 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0332611431630237 0.0541647214889689 1033 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|nuclear replication fork|nucleus|DNA replication preinitiation complex YGL253W HXK2 hom FT CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0332498218813921 0.054210786514066 1034 Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication replicative cell aging|fructose import|regulation of transcription by glucose|glucose import|mannose metabolic process|glycolysis|glucose metabolic process|regulation of cell size|fructose metabolic process hexokinase activity cytosol|mitochondrion|nucleus YLR396C VPS33 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.0332479138564737 0.0542246124662694 1035 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole regulation of SNARE complex assembly|endocytosis|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle fusion with vacuole|Golgi to vacuole transport|vacuole organization|vacuole fusion, non-autophagic|vesicle docking|protein targeting to vacuole phosphatidylinositol binding|ATP binding cytosol|fungal-type vacuole membrane|HOPS complex|fungal-type vacuole|CORVET complex YLR180W SAM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0331946850228086 0.0546115069358894 1036 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cytoplasm YJL181W_p YJL181W hom 0.0331649935081045 0.0549732405987644 1037 Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication biological_process molecular_function cellular_component YGL165C_d YGL165C hom 0.0331642680660252 0.054833624451835 1038 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Unknown Unknown Unknown YLR184W_d YLR184W hom 0.0331095977983362 0.0552347428595183 1039 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR058C APS2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE 0.03308803440808 0.0553936253599368 1040 Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex vesicle-mediated transport molecular_function AP-2 adaptor complex YAL068C PAU8 hom FT TRANSCRIPTION FROM RNA POL II 0.0330317891705197 0.0558464257098303 1041 Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YBR270C_p BIT2 hom 0.0330036576413644 0.056018990601298 1042 Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication biological_process molecular_function cellular_component YCL023C_d YCL023C hom 0.0329968157820541 0.0560699561711883 1043 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Unknown Unknown Unknown YOR371C GPB1 hom FT SIGNALING|PROTEOLYSIS|PLASMA MEMBRANE 0.0329828969693265 0.0561737576767819 1044 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication positive regulation of Ras protein signal transduction|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|negative regulation of phosphorylation|signal transduction|glucose mediated signaling pathway|pseudohyphal growth|invasive growth in response to glucose limitation|negative regulation of Ras protein signal transduction|protein polyubiquitination cAMP-dependent protein kinase inhibitor activity|signal transducer activity|GTPase activating protein binding plasma membrane|cytoplasm YNL078W NIS1 hom FT CELL CYCLE|NUCLEUS|SITE OF POLARIZED GROWTH 0.0329677329669773 0.0562870273491178 1045 Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network regulation of mitosis molecular_function nucleus|cellular bud neck YKR014C YPT52 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0329098539610468 0.0567211114762364 1046 Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress endocytosis|protein targeting to vacuole GTPase activity late endosome YDR163W CWC15 hom FT RNA PROCESSING|NUCLEUS 0.0328618451214788 0.0570832802678128 1047 Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YKL213C DOA1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0328328859504104 0.0577879004950349 1048 WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress double-strand break repair via nonhomologous end joining|ubiquitin-dependent protein catabolic process ubiquitin binding nucleus|cytoplasm YMR096W SNZ1 hom FT COFACTOR METABOLISM 0.0328185767542538 0.0575976581249105 1049 Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins pyridoxine metabolic process|thiamine biosynthetic process protein binding cellular_component YLR309C IMH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0328023050363468 0.057535107306829 1050 Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Golgi to plasma membrane protein transport|vesicle-mediated transport molecular_function cytosol|Golgi apparatus YLR380W CSR1 hom FT KETONE METABOLISM|LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0327458090361312 0.0579665781570131 1051 Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress phosphatidylinositol metabolic process|Golgi to plasma membrane protein transport|Golgi to plasma membrane transport|Unknown|negative regulation of fatty acid biosynthetic process|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cytosol|endosome|mitochondrion|lipid particle YER123W YCK3 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE 0.0326872243802715 0.0586051097360499 1052 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway vesicle-mediated transport|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity fungal-type vacuole membrane|plasma membrane|nucleus|fungal-type vacuole YOR374W ALD4 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0326568847607585 0.0586511525782029 1053 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed NADPH regeneration|ethanol metabolic process|pyruvate metabolic process|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity|aldehyde dehydrogenase (NAD) activity mitochondrial nucleoid|mitochondrion YIL008W URM1 hom FT RNA PROCESSING|CELL DIVISION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0326423360317299 0.058763790747508 1054 Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response cellular response to oxidative stress|tRNA wobble position uridine thiolation|cell budding|protein urmylation|invasive growth in response to glucose limitation|tRNA wobble uridine modification protein tag cytosol|nucleus|cytoplasm YAL045C_d YAL045C hom 0.0326192401221831 0.0589429710084221 1055 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Unknown Unknown Unknown YBL041W PRE7 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0325676598106768 0.0597255278765349 1056 Beta 6 subunit of the 20S proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YHR121W LSM12 hom FT NUCLEUS 0.0325547504284597 0.0594456898823287 1057 Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress RNA metabolic process RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YNL025C SSN8 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.032545834342122 0.0595154727726329 1058 Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance positive regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription by galactose|positive regulation of transcription from RNA polymerase II promoter cyclin-dependent protein kinase regulator activity mediator complex YPR023C EAF3 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0325240070354055 0.0596865937451995 1059 Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition DNA repair|histone acetylation|regulation of DNA-dependent DNA replication initiation|negative regulation of antisense RNA transcription|regulation of transcription from RNA polymerase II promoter|negative regulation of transcription, DNA-dependent|transcription elongation from RNA polymerase II promoter|histone deacetylation histone acetyltransferase activity Rpd3S complex|histone acetyltransferase complex|Rpd3L complex|NuA4 histone acetyltransferase complex YCR065W HCM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0325027448508612 0.0598918289727994 1060 Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role positive regulation of transcription involved in S-phase of mitotic cell cycle|cellular response to oxidative stress|mitochondrion organization|spindle pole body organization|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nuclear chromatin|nucleus|cytoplasm YBR292C_d YBR292C hom 0.0324700713317258 0.0601111871423318 1061 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Unknown Unknown Unknown YMR173W DDR48 hom FT RESPONSE TO DNA DAMAGE 0.0324587405052676 0.0602007032777133 1062 DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress DNA repair ATPase activity|GTPase activity cytoplasm YGR111W_p YGR111W hom FT NUCLEUS 0.0324310296919424 0.0604200906034642 1063 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus regulation of cell size molecular_function nucleus|cytoplasm YER179W DMC1 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0324098150496159 0.0605884948704825 1064 Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p meiosis|reciprocal meiotic recombination|meiotic joint molecule formation double-stranded DNA binding|single-stranded DNA binding condensed nuclear chromosome|nucleus YMR125W STO1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.0322986963563661 0.0615544833551369 1065 Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|mRNA splicing, via spliceosome|response to osmotic stress mRNA binding commitment complex|nuclear cap binding complex|polysome YIL110W HPM1 hom FT NUCLEUS 0.0322646418684772 0.061790223886752 1066 AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance peptidyl-histidine methylation, to form tele-methylhistidine S-adenosylmethionine-dependent methyltransferase activity|protein-histidine N-methyltransferase activity nucleus|cytoplasm YDL118W_d YDL118W hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0322569831712176 0.0618132156591748 1067 Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component YLR288C MEC3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0322191135335237 0.0621198304602608 1068 DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 telomere maintenance via recombination|DNA damage checkpoint|DNA metabolic process|chromatin silencing at telomere DNA binding nucleus|checkpoint clamp complex YDL152W_d YDL152W het FF 0.0322102403574732 0.0621918544965792 1069 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Unknown Unknown Unknown YOR157C PUP1 het FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.032104975224752 0.0634462068010828 1070 Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YMR015C ERG5 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0320554255322695 0.0634596474769909 1071 C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs ergosterol biosynthetic process C-22 sterol desaturase activity endoplasmic reticulum YLR165C PUS5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0320260581749977 0.0637025317615 1072 Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA modification|pseudouridine synthesis pseudouridylate synthase activity mitochondrion YMR150C IMP1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0320104430153737 0.0638319900093269 1073 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein processing involved in protein targeting to mitochondrion endopeptidase activity mitochondrion|mitochondrial inner membrane peptidase complex YFR032C-B_p YFR032C-B hom 0.0320070633368205 0.063860037972697 1074 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR524W-C_p YDR524W-C hom 0.03191987148435 0.0647869459392282 1075 Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin biological_process molecular_function cellular_component YDL012C YDL012C hom FT PLASMA MEMBRANE 0.0319137073412306 0.0646388305560661 1076 Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication biological_process molecular_function plasma membrane YPL121C MEI5 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0318824509930132 0.0649013207681507 1077 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p meiotic DNA recombinase assembly|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YIL034C CAP2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0318715459936338 0.0649931073275259 1078 Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping|filamentous growth actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YIL148W RPL40A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION|NUCLEUS|RIBOSOME 0.0318211583153643 0.0654588089661546 1079 Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress cytoplasmic translation|protein ubiquitination|ribosome biogenesis structural constituent of ribosome|protein tag cytosolic large ribosomal subunit YKR018C YKR018C hom FT NUCLEUS|MITOCHONDRION 0.0318158404375115 0.065463648149468 1080 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YDR357C_p CNL1 hom 0.0316966158543242 0.0665207462785612 1081 Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function BLOC-1 complex|cytoplasm YAL053W FLC2 hom FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0316760208787797 0.0666570716807829 1082 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication protein folding|fungal-type cell wall biogenesis|FAD transport|transmembrane transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|cytoplasm YDR177W UBC1 het FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS 0.0316596402840994 0.0667980521178917 1083 Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress spore germination|vesicle organization|endocytosis|ER-associated protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity proteasome complex YLR385C SWC7 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0316583466099576 0.0668091966395355 1084 Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin chromatin remodeling molecular_function Swr1 complex|nucleus YLR193C UPS1 hom FT LIPID METABOLISM|MITOCHONDRION 0.0315500283164532 0.0677477608250768 1085 Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Unknown|cardiolipin metabolic process molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial intermembrane space YJL136C RPS21B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0315301184925436 0.0679214509145598 1086 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytoplasmic translation|endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YGL179C TOS3 hom FT CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION 0.0315158001820554 0.0680465877071643 1087 Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome protein phosphorylation|glucose metabolic process protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YIL059C_d YIL059C hom 0.0314617727094859 0.0685204757650864 1088 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Unknown Unknown Unknown YNR052C POP2 hom FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|SITE OF POLARIZED GROWTH 0.0313728209004598 0.0693065983351725 1089 RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter 3'-5'-exoribonuclease activity CCR4-NOT core complex|mating projection tip|cytoplasmic mRNA processing body|cytoplasm YJR024C MDE1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.031342974649492 0.0695720213807495 1090 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant L-methionine salvage from methylthioadenosine methylthioribulose 1-phosphate dehydratase activity cytoplasm YGR132C PHB1 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0313346280265513 0.0699387141912852 1091 Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation mitochondrion inheritance|protein folding|negative regulation of proteolysis|replicative cell aging|mitochondrion morphogenesis|inner mitochondrial membrane organization molecular_function mitochondrion|mitochondrial inner membrane YNL042W-B_p YNL042W-B hom 0.0312986295762826 0.0699679226627899 1092 Putative protein of unknown function biological_process molecular_function cellular_component YCL054W SPB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.031214816807827 0.070763273505028 1093 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA methylation rRNA (uridine-2'-O-)-methyltransferase activity|rRNA (guanine) methyltransferase activity nucleolus|nucleus|preribosome, large subunit precursor YGR246C BRF1 het FT TRANSLATION|NUCLEUS 0.0312079987551266 0.0707828002827762 1094 TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB DNA-dependent transcriptional open complex formation|transposon integration|RNA polymerase III transcriptional preinitiation complex assembly|negative regulation of protein homodimerization activity TFIIIC-class transcription factor binding|RNA polymerase III core binding|DNA binding|TFIIIB-type transcription factor activity transcription factor TFIIIB complex YKR088C TVP38 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0311787864130725 0.0710471054807256 1095 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vesicle-mediated transport|mitotic spindle elongation molecular_function integral to membrane|integral to Golgi membrane|cytoplasm YMR129W POM152 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|NUCLEAR PORE 0.031125881283912 0.0715278349681343 1096 Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 nucleocytoplasmic transport|nuclear pore organization|protein import into nucleus|spindle pole body duplication associated with nuclear envelope protein anchor|structural constituent of nuclear pore mitochondrion|integral to membrane|nuclear pore transmembrane ring|nuclear envelope lumen|nuclear pore YER091C-A_d YER091C-A hom 0.0311145938353703 0.071630743962049 1097 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL167C PMR1 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0311073123543544 0.0716971943998628 1098 High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease manganese ion transport|calcium ion transport|exocytosis manganese-transporting ATPase activity|calcium-transporting ATPase activity|calcium ion binding integral to membrane|Golgi membrane YJR149W_p YJR149W hom FT OXIDATION-REDUCTION PROCESS 0.0311012804487347 0.0718795355392152 1099 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YPR003C_p YPR003C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0310687978636848 0.0720495159593664 1100 Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YFR047C BNA6 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.031039163997771 0.0723215633936912 1101 Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p de novo NAD biosynthetic process from tryptophan nicotinate-nucleotide diphosphorylase (carboxylating) activity nucleus|cytoplasm YER049W TPA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0310218334011633 0.0727800618046973 1102 Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|translational termination poly(A) RNA binding nucleus YDR007W TRP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0309359745861049 0.0732754396411851 1103 Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) tryptophan biosynthetic process phosphoribosylanthranilate isomerase activity cytoplasm YDR328C SKP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|ION HOMEOSTASIS|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|NUCLEUS|CHROMOSOME|UBIQUITIN LIGASE COMPLEX|KINETOCHORE 0.030935654824562 0.0732784114068556 1104 Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress kinetochore assembly|protein complex assembly|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|regulation of protein complex assembly|G1/S transition of mitotic cell cycle|septin ring assembly|regulation of exit from mitosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein neddylation|vacuolar acidification|cytokinesis ubiquitin-protein ligase activity|DNA replication origin binding|protein binding RAVE complex|SCF ubiquitin ligase complex|nucleus|CBF3 complex|kinetochore|cytoplasm YDL088C ASM4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0308249857053002 0.0743128709599956 1105 FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication nuclear pore organization|mRNA export from nucleus in response to heat stress|mitotic cell cycle spindle assembly checkpoint nucleocytoplasmic transporter activity|phospholipid binding|single-stranded DNA binding nuclear pore central transport channel|integral to membrane|nuclear pore YML035C AMD1 hom FT NUCLEOTIDE METABOLISM 0.0308129961757605 0.0748596170587869 1106 AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools guanine salvage|purine nucleotide metabolic process AMP deaminase activity cytoplasm YGL096W TOS8 hom FT NUCLEUS|CHROMOSOME 0.03077392720571 0.0747941361321726 1107 Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication biological_process chromatin binding|sequence-specific DNA binding nuclear chromatin YOR037W CYC2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.03076528682903 0.0748758294421065 1108 Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) cytochrome c-heme linkage|mitochondrial membrane organization oxidoreductase activity extrinsic to mitochondrial inner membrane|mitochondrion YKL129C MYO3 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON 0.0306792761219046 0.0758679259740873 1109 One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization bipolar cellular bud site selection|fungal-type cell wall organization|endocytosis|exocytosis|actin cortical patch localization|response to osmotic stress microfilament motor activity actin cortical patch YLR379W_d YLR379W het 0.0306717491957869 0.0757648831060037 1110 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Unknown Unknown Unknown YLR223C IFH1 het FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0306708057232473 0.0757738942665042 1111 Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|chromatin silencing at telomere transcription coactivator activity nuclear chromatin|nucleolus|nucleus|CURI complex YJR147W HMS2 hom FT NUCLEUS 0.0306500344890541 0.0759725030882599 1112 Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication pseudohyphal growth molecular_function nucleus|cytoplasm YGL047W ALG13 het FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0306481677464195 0.0759903731381526 1113 Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress dolichol-linked oligosaccharide biosynthetic process N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity cytosol|UDP-N-acetylglucosamine transferase complex|extrinsic to endoplasmic reticulum membrane|endoplasmic reticulum|nucleus|cytoplasm YLR430W SEN1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0306347558517303 0.0761188640161589 1114 Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS tRNA processing|mRNA 3'-end processing|termination of RNA polymerase II transcription|mRNA polyadenylation|snoRNA 3'-end processing|rRNA processing|snRNA processing ATP-dependent 5'-3' DNA helicase activity|DNA-dependent ATPase activity|RNA-dependent ATPase activity|ATP-dependent 5'-3' RNA helicase activity|protein domain specific binding Nrd1 complex|nucleus YDR313C PIB1 hom 0.0306264182149232 0.0762426779186129 1115 RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain protein ubiquitination ubiquitin-protein ligase activity|phosphatidylinositol-3-phosphate binding fungal-type vacuole membrane|late endosome YOR260W GCD1 het FT TRANSLATION 0.0305915407757267 0.0765780398979477 1116 Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation translation initiation factor activity|guanyl-nucleotide exchange factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YBR263W SHM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0305329856615554 0.077099641963175 1117 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine serine family amino acid biosynthetic process|one-carbon metabolic process glycine hydroxymethyltransferase activity mitochondrion YDR099W BMH2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0305253448651621 0.0771736912616256 1118 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|ascospore formation|Ras protein signal transduction|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|DNA damage checkpoint|DNA replication initiation|signal transduction involved in filamentous growth|pseudohyphal growth|fungal-type cell wall chitin biosynthetic process|pre-replicative complex assembly DNA replication origin binding|phosphoserine binding nucleus|plasma membrane YCR017C CWH43 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.030443706367396 0.0779684964847044 1119 Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion fungal-type cell wall organization|GPI anchor biosynthetic process molecular_function integral to membrane|integral to plasma membrane|cellular bud neck|cellular bud tip YKL087C CYT2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION 0.0303239490052105 0.0791464561703377 1120 Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 cytochrome c-heme linkage holocytochrome-c synthase activity mitochondrion|mitochondrial intermembrane space YDR499W LCD1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0302514244702114 0.0798668298378182 1121 Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress establishment of protein localization|telomere maintenance via telomerase|DNA damage checkpoint damaged DNA binding nuclear chromosome YBL013W FMT1 hom FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.030186952119125 0.0805116863408436 1122 Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate translational initiation|methionyl-tRNA aminoacylation methionyl-tRNA formyltransferase activity mitochondrion YML012C-A_d YML012C-A hom FF 0.0301448462084557 0.0809351094875321 1123 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Unknown Unknown Unknown YJL080C SCP160 hom FT CELL CYCLE|SIGNALING|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.030106964475065 0.0813630404859527 1124 Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins meiotic telomere clustering|pheromone-dependent signal transduction involved in conjugation with cellular fusion|chromosome segregation|intracellular mRNA localization|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere G-protein alpha-subunit binding|RNA binding endoplasmic reticulum membrane|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YFR050C PRE4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0300961200698856 0.0815638752544184 1125 Beta 7 subunit of the 20S proteasome proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YPL265W DIP5 hom FT PLASMA MEMBRANE 0.0300319409867518 0.0820794260203874 1126 Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress amino acid transport amino acid transmembrane transporter activity integral to membrane|plasma membrane YBR050C REG2 hom FT TRANSCRIPTION FROM RNA POL II 0.0300218221498082 0.0821826196197259 1127 Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease negative regulation of transcription from RNA polymerase II promoter protein phosphatase type 1 regulator activity protein phosphatase type 1 complex YLR142W PUT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0299613998590697 0.0828468991931523 1128 Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source glutamate biosynthetic process|proline catabolic process to glutamate proline dehydrogenase activity mitochondrion YAL034W-A MTW1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0298962546514388 0.0834719479373232 1129 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly chromosome segregation|protein localization to kinetochore molecular_function nuclear MIS12/MIND type complex|spindle pole YER071C_p TDA2 hom FT SITE OF POLARIZED GROWTH 0.0298947278359812 0.0839035935239207 1130 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function mating projection tip|cytoplasm YJL050W MTR4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|OXIDATION-REDUCTION PROCESS|RNA LOCALIZATION|NUCLEUS 0.0298947044761579 0.0834879668938183 1131 ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent 3'-5' RNA helicase activity|poly(A) RNA binding nucleolus|nucleus|TRAMP complex YDL150W RPC53 het FF|FT NUCLEUS 0.0298325206557356 0.084132607125872 1132 RNA polymerase III subunit C53 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YDR430C CYM1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0296523464360568 0.0860231802559333 1133 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis|proteolysis involved in cellular protein catabolic process metalloendopeptidase activity mitochondrion|mitochondrial intermembrane space YGR071C ENV11 hom FT VACUOLAR PROTEIN PROCESSING|NUCLEUS 0.0296521105855623 0.0860256773416663 1134 Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus vacuole organization|vacuolar protein processing molecular_function nucleus YDR009W GAL3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0296371268007351 0.0863721657976224 1135 Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication maintenance of protein location|galactose metabolic process|positive regulation of transcription from RNA polymerase II promoter by galactose protein binding nucleus|cytoplasm YJR140C HIR3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0295917705375886 0.0866664581165358 1136 Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding|transcription corepressor activity HIR complex|nucleus YLR390W-A CCW14 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0295852709536516 0.0869240743096122 1137 Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall fungal-type cell wall organization structural constituent of cell wall mitochondrion|fungal-type cell wall YGL169W SUA5 het FT RNA PROCESSING|TRANSLATION|NUCLEUS|CHROMOSOME 0.029490381428008 0.0879888135751061 1138 Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family threonylcarbamoyladenosine metabolic process|telomere maintenance|regulation of translational fidelity single-stranded telomeric DNA binding cytoplasm YPL188W POS5 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0294619221525167 0.0880584475085675 1139 Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress cellular response to oxidative stress|NADP biosynthetic process NADH kinase activity mitochondrion|mitochondrial matrix YDR044W HEM13 het FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.0294483983037006 0.0883469500410338 1140 Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) heme biosynthetic process coproporphyrinogen oxidase activity cytosol YOR038C HIR2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS 0.0294276850810867 0.0885711454545371 1141 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p DNA replication-independent nucleosome assembly|regulation of transcription from RNA polymerase II promoter|transcription elongation from RNA polymerase II promoter nucleosome binding|transcription corepressor activity|DNA binding HIR complex|nucleus YML053C_p YML053C hom FT NUCLEUS 0.029427040960253 0.0884829689185538 1142 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus|cytoplasm YOR325W_d YOR325W hom 0.0293731765103121 0.0890201362701365 1143 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Unknown Unknown Unknown YDR196C CAB5 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0293285004292814 0.0895074576237258 1144 Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies coenzyme A biosynthetic process dephospho-CoA kinase activity nuclear envelope|mitochondrion|endoplasmic reticulum YKL073W LHS1 hom FT PROTEIN LOCALIZATION|NUCLEOTIDE METABOLISM|ENDOPLASMIC RETICULUM 0.0293209342356046 0.0895902009858299 1145 Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway posttranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|adenyl-nucleotide exchange factor activity|ATP binding endoplasmic reticulum lumen YDR438W THI74 hom FT MITOCHONDRION 0.0292489941999499 0.0903800112488004 1146 Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication biological_process molecular_function mitochondrion|integral to membrane YML058W SML1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|NUCLEUS 0.0291759161886649 0.0911880386620116 1147 Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase mitochondrion organization|response to DNA damage stimulus enzyme inhibitor activity nucleus|cytoplasm YDL039C PRM7 hom 0.0291711540171741 0.0912408950833264 1148 Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements conjugation with cellular fusion molecular_function integral to membrane YDR075W PPH3 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0291671761268071 0.0912850654601831 1149 Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Unknown|signal transduction involved in meiotic recombination checkpoint|double-strand break repair via homologous recombination|protein dephosphorylation|positive regulation of nitrogen compound metabolic process protein serine/threonine phosphatase activity condensed nuclear chromosome|nucleus|cytoplasm YMR215W GAS3 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0291224876470244 0.0917824667240788 1150 Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation biological_process 1,3-beta-glucanosyltransferase activity fungal-type cell wall|integral to membrane|plasma membrane YBR229C ROT2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0290555677557056 0.0925313809359672 1151 Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations fungal-type cell wall biogenesis alpha-glucosidase activity glucosidase II complex|mitochondrion|endoplasmic reticulum|endoplasmic reticulum lumen YJR093C FIP1 het FT RNA PROCESSING|NUCLEUS 0.0290474499537699 0.0926225615458924 1152 Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p mRNA cleavage|mRNA polyadenylation RNA binding|protein binding, bridging mRNA cleavage and polyadenylation specificity factor complex YML119W_p YML119W hom 0.0290326176233643 0.0927893471766738 1153 Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate biological_process molecular_function cellular_component YPL214C THI6 hom 0.0289424731036089 0.0940044489271166 1154 Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern thiamine biosynthetic process hydroxyethylthiazole kinase activity|thiamine-phosphate diphosphorylase activity cytosol|cytoplasm YKR054C DYN1 hom FT CELL CYCLE|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0289233936960562 0.0940249748481711 1155 Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p nuclear migration along microtubule|establishment of mitotic spindle orientation|mitotic spindle elongation|mitotic sister chromatid segregation microtubule motor activity cytoplasmic dynein complex|cell cortex|cytoplasmic microtubule|spindle pole body|astral microtubule YMR243C ZRC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.028906899616606 0.0942127098752681 1156 Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication cobalt ion transport|cellular zinc ion homeostasis|zinc ion transport|glutathione metabolic process zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity integral to membrane|fungal-type vacuole YOR086C TCB1 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.0289012690739767 0.09427686524105 1157 Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding mitochondrion|plasma membrane|cortical endoplasmic reticulum YFL044C OTU1 hom FT PROTEOLYSIS|NUCLEUS 0.0288911186844403 0.0944417896376326 1158 Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress protein deubiquitination|regulation of transcription, DNA-dependent ubiquitin-specific protease activity nucleus|cytoplasm YMR322C_p SNO4 hom FT PROTEOLYSIS 0.0288258598854654 0.0951394688735923 1159 Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation biological_process cysteine-type peptidase activity cellular_component YDL201W TRM8 hom FT RNA PROCESSING|NUCLEUS 0.028788722218253 0.095566601970433 1160 Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YDL017W CDC7 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|PROTEIN PHOSPHORYLATION|GENE SILENCING|NUCLEUS 0.0287628964809943 0.095864536387196 1161 DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p negative regulation of exit from mitosis|regulation of chromatin silencing at telomere|protein phosphorylation|double-strand break repair via break-induced replication|DNA replication initiation protein serine/threonine kinase activity nucleus|Dbf4-dependent protein kinase complex YDR245W MNN10 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0287590100319418 0.0960582741082541 1162 Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family N-glycan processing|cell wall mannoprotein biosynthetic process|substituted mannan metabolic process|barrier septum assembly alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YLR323C CWC24 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0287551340765058 0.0959542312170882 1163 Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p generation of catalytic spliceosome for first transesterification step|snoRNA splicing molecular_function nucleus|U2-type spliceosomal complex YNL228W_d YNL228W hom 0.0287522131987622 0.0959879994327354 1164 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Unknown Unknown Unknown YHR126C_p ANS1 hom FT PLASMA MEMBRANE 0.0287513415070427 0.0959980788870319 1165 Putative GPI protein; transcription dependent upon Azf1p biological_process molecular_function cellular_component YMR089C YTA12 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0287184028089943 0.0963795731085417 1166 Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes proteolysis|signal peptide processing|protein complex assembly|protein import into mitochondrial intermembrane space metallopeptidase activity|protein binding|ATPase activity|ATP binding mitochondrion|mitochondrial inner boundary membrane|mitochondrial inner membrane|m-AAA complex|cytoplasm YER112W LSM4 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0286823980506697 0.0968477830341409 1167 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|cytoplasmic mRNA processing body assembly U6 snRNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YJR103W URA8 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0286654431419192 0.0969954899780985 1168 Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YDL133C-A RPL41B hom FT TRANSLATION|RIBOSOME 0.0286339686051469 0.0973630243169546 1169 Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YBR140C IRA1 het FF|FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0286160473636729 0.0975727908245124 1170 GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication negative regulation of cAMP biosynthetic process|negative regulation of Ras protein signal transduction|regulation of adenylate cyclase activity|positive regulation of Ras GTPase activity Ras GTPase activator activity mitochondrion|integral to membrane|membrane YGR128C UTP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.0286023177630894 0.0977337386723122 1171 Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|tRNA export from nucleus snoRNA binding|tRNA binding small-subunit processome|t-UTP complex|nucleolus|rDNA heterochromatin|90S preribosome YKL205W LOS1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0285919814570922 0.0980054158049782 1172 Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm tRNA re-export from nucleus|tRNA splicing, via endonucleolytic cleavage and ligation|tRNA export from nucleus tRNA binding|Ran GTPase binding mitochondrion|integral to membrane|nuclear matrix|cytoplasm YPL196W OXR1 hom FT RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0285420044820756 0.0984432859502472 1173 Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes cellular response to oxidative stress molecular_function mitochondrion YBR238C YBR238C hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0285104020556446 0.0988167066759032 1174 Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span aerobic respiration molecular_function mitochondrion|integral to mitochondrial membrane|cytoplasm YER062C HOR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0284985202368024 0.0989573966977415 1175 Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication cellular carbohydrate metabolic process|glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YDL224C WHI4 hom 0.0284790406651545 0.0991883957741366 1176 Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication regulation of cell size RNA binding cytoplasm YLR300W EXG1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0284718747601084 0.0992734810105764 1177 Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes fungal-type cell wall organization|cellular glucan metabolic process glucan exo-1,3-beta-glucosidase activity extracellular region|fungal-type cell wall YBR089W_d YBR089W het 0.0284220182387472 0.0998670710137207 1178 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Unknown Unknown Unknown YOR360C PDE2 hom FT SIGNALING|NUCLEUS 0.0284063301122924 0.100054437414798 1179 High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon cAMP-mediated signaling 3',5'-cyclic-AMP phosphodiesterase activity nucleus|cytoplasm YCR088W ABP1 hom FT PROTEIN LOCALIZATION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.028364292765154 0.100557879201362 1180 Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences protein localization|actin cortical patch assembly actin filament binding cell cortex|mating projection tip|cytoplasm|actin cortical patch YBR165W UBS1 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.0283570598959769 0.100644703609639 1181 Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity protein export from nucleus|protein ubiquitination molecular_function nucleus YNL290W RFC3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0283500985914101 0.101135757610393 1182 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity|ATPase activity Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|nucleus|Ctf18 RFC-like complex YJL075C_d APQ13 hom 0.0283446509646288 0.100793801320295 1183 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Unknown Unknown Unknown YHR181W SVP26 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0283288044982064 0.100984457952 1184 Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment protein retention in Golgi apparatus|fungal-type cell wall organization|protein glycosylation|ER to Golgi vesicle-mediated transport COPII adaptor activity integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|integral to Golgi membrane|ER to Golgi transport vesicle YML131W YML131W hom FT OXIDATION-REDUCTION PROCESS 0.028324422722575 0.101037227904278 1185 Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm YGR193C PDX1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.028324349163753 0.101089023073195 1186 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core acetyl-CoA biosynthetic process from pyruvate structural molecule activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YNL323W LEM3 hom FF|FT MEMBRANE ORGANIZATION|SIGNALING|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0282896380671163 0.102689044394411 1187 Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane phospholipid translocation|cell surface receptor signaling pathway phospholipid-translocating ATPase activity integral to membrane|endoplasmic reticulum|plasma membrane|cytoplasm YBR215W HPC2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0282565169066694 0.1018578314002 1188 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding HIR complex|nucleus YHR188C GPI16 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0282354644887425 0.102113312137551 1189 Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog attachment of GPI anchor to protein GPI-anchor transamidase activity GPI-anchor transamidase complex YLR254C NDL1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CYTOSKELETON 0.0282074712049852 0.102453812739055 1190 Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends nuclear migration along microtubule microtubule plus-end binding cytoplasmic microtubule|nucleus|cytoplasm YMR008C PLB1 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0281765809412359 0.102830598508323 1191 Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol glycerophospholipid metabolic process lysophospholipase activity periplasmic space|plasma membrane YLR168C UPS2 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0281524788531477 0.103125349520011 1192 Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI cristae formation|Unknown|phosphatidylethanolamine metabolic process|cardiolipin metabolic process molecular_function extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial intermembrane space YMR095C SNO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0280879683482394 0.103917570619963 1193 Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase pyridoxine metabolic process|thiamine biosynthetic process|anti-apoptosis molecular_function cytoplasm YBR065C ECM2 hom FT RNA PROCESSING|NUCLEUS 0.0280094317986279 0.10488855409994 1194 Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p mRNA splicing, via spliceosome molecular_function nucleus YLR306W UBC12 hom 0.0279870320644807 0.105478810176557 1195 Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes protein neddylation NEDD8 ligase activity cellular_component YDR201W SPC19 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0279401556344696 0.105751010511811 1196 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|condensed nuclear chromosome kinetochore|DASH complex|spindle pole body|spindle YHR023W MYO1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0279193983811089 0.106010520678339 1197 Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively cytokinesis, actomyosin contractile ring assembly|cytokinesis, completion of separation|actomyosin contractile ring contraction|cytokinesis microfilament motor activity cellular bud neck contractile ring|incipient cellular bud site|cellular bud neck|myosin II complex YLR240W VPS34 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|GOLGI APPARATUS 0.0279118549995562 0.106104954084186 1198 Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery peroxisome degradation|phosphatidylinositol phosphorylation|protein phosphorylation 1-phosphatidylinositol-3-kinase activity|protein kinase activity endosome|fungal-type vacuole membrane|phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|peroxisome|pre-autophagosomal structure YBR084C-A RPL19A hom FT TRANSLATION|RIBOSOME 0.0279009414542468 0.107132753449472 1199 Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YBR058C-A TSC3 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0278921903240982 0.106351443933454 1200 Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis 3-keto-sphinganine metabolic process|sphingolipid biosynthetic process enzyme activator activity SPOTS complex YGL181W GTS1 hom FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS|CYTOSKELETON 0.0278846529263748 0.106446042795144 1201 Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations endocytosis|ultradian rhythm|regulation of transcription from RNA polymerase II promoter|negative regulation of phosphatidylinositol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter ARF GTPase activator activity|repressing transcription factor binding|transcription factor binding transcription factor activity nucleus|cytoplasm|actin cortical patch YDR094W_d YDR094W hom 0.0278562704502203 0.106802858254599 1202 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Unknown Unknown Unknown YOL120C RPL18A het FF|FT TRANSLATION|RIBOSOME 0.027844220271131 0.106954635494689 1203 Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR305C STT4 het FT LIPID METABOLISM|SIGNALING|MITOCHONDRION|PLASMA MEMBRANE 0.0277958015769743 0.107566212884252 1204 Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity mitochondrion|plasma membrane YER060W-A FCY22 hom 0.0276532807524506 0.109806724308163 1205 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YKL090W CUE2 hom 0.0276432766791538 0.109510866492665 1206 Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination biological_process ubiquitin binding cellular_component YGR122C-A_d YGR122C-A hom 0.0275439891702898 0.110791613379369 1207 Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Unknown Unknown Unknown YJL189W RPL39 hom FT TRANSLATION|RIBOSOME 0.027534903288866 0.110909403553886 1208 Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR312W SSF2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0275284868211447 0.111419882429693 1209 Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication conjugation with cellular fusion|ribosomal large subunit assembly rRNA binding nucleolus YIL001W_p YIL001W hom 0.0275019710679928 0.111337168254915 1210 Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm biological_process molecular_function cytoplasm YKL072W STB6 hom 0.0274843065968094 0.111567152105569 1211 Protein that binds Sin3p in a two-hybrid assay biological_process molecular_function cellular_component YLR399C BDF1 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0274699045201481 0.111808418439232 1212 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p DNA repair|snRNA transcription|regulation of chromatin silencing at telomere|regulation of transcription by chromatin organization|regulation of chromatin silencing at silent mating-type cassette|Unknown|chromatin remodeling|negative regulation of heterochromatin assembly histone acetyl-lysine binding|chromatin binding|core promoter binding|TFIID-class transcription factor binding nuclear chromatin|Swr1 complex YLL063C AYT1 hom 0.027433345380645 0.112232745431633 1213 Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis secondary metabolic process trichothecene 3-O-acetyltransferase activity cellular_component YBR051W_d YBR051W hom 0.0274234297069392 0.112362614896099 1214 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Unknown Unknown Unknown YBR276C PPS1 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0274093352297589 0.112547419582439 1215 Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle inactivation of MAPK activity|protein dephosphorylation|regulation of S phase of mitotic cell cycle protein tyrosine/serine/threonine phosphatase activity cellular_component YMR314W PRE5 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0273830818664752 0.113161262894668 1216 Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YDR451C YHP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0273712468936318 0.113048026478343 1217 Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II regulatory region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nuclear chromatin|nucleus YIL073C SPO22 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0273643832671744 0.113569565997303 1218 Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis regulation of synaptonemal complex assembly molecular_function condensed nuclear chromosome YLR247C IRC20 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0273590001710602 0.113209361071734 1219 Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci double-strand break repair via synthesis-dependent strand annealing helicase activity mitochondrion|nucleus YIL111W COX5B hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0272979231377501 0.11401668008743 1220 Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity|nitrite reductase (NO-forming) activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDR207C UME6 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|CHROMOSOME 0.0272969439367947 0.114191726697075 1221 Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p spore germination|positive regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of transcription from RNA polymerase II promoter during meiosis|lipid particle organization|nitrogen catabolite repression of transcription from RNA polymerase II promoter|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter during mitosis|Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|repressing transcription factor binding|transcription factor binding transcription factor activity Rpd3L-Expanded complex|Rpd3L complex YBR168W PEX32 hom FT PEROXISOME ORGANIZATION 0.0272942721292209 0.11476931153518 1222 Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function integral to membrane|integral to peroxisomal membrane YLR414C PUN1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0272329330367798 0.114880690413651 1223 Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress fungal-type cell wall organization|protein oligomerization molecular_function integral to membrane|membrane raft|plasma membrane|cellular bud|cytoplasm YMR003W AIM34 hom FT MITOCHONDRION 0.0272292029092779 0.114930436367742 1224 Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YER067W RGI1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|PLASMA MEMBRANE 0.0272069705431623 0.11522728442725 1225 Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress energy reserve metabolic process molecular_function cell periphery|nucleus|cytoplasm YJR002W MPP10 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0271604862402171 0.115849893300413 1226 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|Mpp10 complex|nucleolus|nucleus|90S preribosome YBR283C SSH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0271554815326532 0.115917083454789 1227 Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential SRP-dependent cotranslational protein targeting to membrane protein transmembrane transporter activity|signal sequence binding integral to membrane|Ssh1 translocon complex YAL019W FUN30 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0270586680393837 0.117222875914881 1228 Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate chromosome organization|chromatin silencing at rDNA|heterochromatin maintenance involved in chromatin silencing|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette|heterochromatin assembly involved in chromatin silencing chromatin binding|DNA-dependent ATPase activity|DNA binding|protein homodimerization activity mitochondrion|mating-type region heterochromatin|nucleus YPL056C_p LCL1 hom FT PLASMA MEMBRANE 0.0270516421576668 0.117372804277002 1229 Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan biological_process molecular_function cellular_component YLR028C ADE16 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0270417002400765 0.117452916909788 1230 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine ascospore formation|'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process|aerobic respiration phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol YHR083W SAM35 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0270039867712567 0.117965485758689 1231 Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane molecular_function mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion YLL038C ENT4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0269308906602166 0.118963937993839 1232 Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome endocytosis|actin filament organization|establishment of protein localization to plasma membrane|actin cortical patch assembly clathrin binding actin cortical patch YBR266C_d SLM6 hom 0.0269230728104169 0.119071116249435 1233 Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Unknown Unknown Unknown YGR251W NOP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0269143378848677 0.119190956550883 1234 Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|nucleolus|nucleus|90S preribosome YKL086W SRX1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0268966740342496 0.119433587456121 1235 Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress cellular response to oxidative stress sulfiredoxin activity nucleus|cytoplasm YDR299W BFR2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.02688060711592 0.119654618653694 1236 Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock rRNA processing molecular_function nucleolus|90S preribosome YDL121C_p YDL121C hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0268518861727634 0.120716027869674 1237 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein biological_process molecular_function endoplasmic reticulum YDR329C PEX3 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOPLASMIC RETICULUM 0.02683432742885 0.120403810895374 1238 Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization protein binding peroxisomal membrane|endoplasmic reticulum YMR135W-A_d YMR135W-A hom 0.026798347499962 0.121124455651392 1239 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER072W VTC1 hom FT MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.0267793048662221 0.121055608985783 1240 Subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress polyphosphate metabolic process|vacuolar transport|microautophagy|vacuole fusion, non-autophagic mRNA binding integral to membrane|endoplasmic reticulum|vacuolar transporter chaperone complex|intrinsic to vacuolar membrane YPL017C IRC15 hom FT PROTEIN LOCALIZATION|CELL CYCLE|OXIDATION-REDUCTION PROCESS|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0267668910776732 0.121228167953078 1241 Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci microtubule nucleation|mitotic recombination|microtubule anchoring|meiotic sister chromatid segregation|attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation|positive regulation of mitotic cell cycle microtubule binding|S-adenosylmethionine-dependent methyltransferase activity microtubule|cytoplasm YDL219W DTD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0267575688384025 0.121357878965591 1242 D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Unknown D-leucyl-tRNA(Leu) deacylase activity|D-tyrosyl-tRNA(Tyr) deacylase activity cytoplasm YDR355C_d YDR355C het 0.0267571181710914 0.121809574383403 1243 Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Unknown Unknown Unknown YAL044W-A_p YAL044W-A hom 0.0266825923244475 0.122405062571204 1244 Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein biological_process molecular_function cellular_component YBR097W VPS15 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|PROTEIN PHOSPHORYLATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0266742980196891 0.122521339909968 1245 Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery protein retention in Golgi apparatus|vacuole inheritance|peroxisome degradation|protein phosphorylation|macroautophagy|inositol lipid-mediated signaling|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding|protein serine/threonine kinase activity mitochondrion|phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|Golgi membrane YPL046C ELC1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0266026829885105 0.123640979108028 1246 Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair global genome nucleotide-excision repair|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YFL024C EPL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0266017312660811 0.124611727136549 1247 Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YIL137C TMA108 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|PROTEOLYSIS 0.0265982134081235 0.123872474021602 1248 Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease ribosome biogenesis molecular_function ribosome|cytoplasm YOR159C SME1 het FT RNA PROCESSING|NUCLEUS 0.0265959153813363 0.123624445157512 1249 Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YMR013C SEC59 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0265436487082088 0.124364307586639 1250 Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation protein glycosylation dolichol kinase activity integral to membrane|endoplasmic reticulum membrane YLR086W SMC4 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0265372081343847 0.124455715750577 1251 Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate mitotic chromosome condensation|mitotic sister chromatid segregation|tRNA gene clustering chromatin binding|ATPase activity SMC loading complex|nuclear condensin complex YDL113C ATG20 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0265164474398796 0.124750719527909 1252 Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate mitochondrion degradation|early endosome to Golgi transport|macroautophagy|CVT pathway phosphatidylinositol-3-phosphate binding endosome|pre-autophagosomal structure|extrinsic to membrane YDR469W SDC1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0265055274774441 0.124906107792667 1253 Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 histone H3-K4 methylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YDR289C RTT103 hom FT RNA PROCESSING|NUCLEUS|CHROMOSOME 0.0264957180450603 0.125045822074699 1254 Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated|mRNA 3'-end processing RNA polymerase II core binding nuclear chromatin|site of double-strand break YOR100C CRC1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0264822997545755 0.125237133973139 1255 Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation fatty acid metabolic process carnitine:acyl carnitine antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YKL140W TGL1 hom FT LIPID METABOLISM 0.0264798333497218 0.125272323652344 1256 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes cellular lipid metabolic process|sterol metabolic process lipase activity|sterol esterase activity integral to membrane|lipid particle YLR401C DUS3 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0264441891555495 0.12578174133878 1257 Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress tRNA modification tRNA dihydrouridine synthase activity nucleus|cytoplasm YGR181W TIM13 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0264037709617741 0.12636133797317 1258 Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity mitochondrion|integral to membrane|mitochondrial intermembrane space protein transporter complex|mitochondrial intermembrane space YNL301C RPL18B hom FT TRANSLATION|RIBOSOME 0.0263154146139207 0.127635605076781 1259 Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YBR248C HIS7 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0262749504949393 0.128450537520671 1260 Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor purine nucleoside monophosphate biosynthetic process|histidine biosynthetic process imidazoleglycerol-phosphate synthase activity intracellular YIL083C CAB2 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0262706681504521 0.128284734071931 1261 Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) coenzyme A biosynthetic process phosphopantothenate--cysteine ligase activity nucleus|cytoplasm YJL158C CIS3 hom FT CELL WALL ORG/BIOGENESIS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0262343461295234 0.128813535020623 1262 Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family fungal-type cell wall organization structural constituent of cell wall extracellular region|fungal-type cell wall|endoplasmic reticulum|plasma membrane|cellular bud tip YIR026C YVH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|SIGNALING|CELL WALL ORG/BIOGENESIS|NUCLEUS 0.0261996009882868 0.129320961455292 1263 Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases meiosis|ascospore wall assembly|ribosomal large subunit assembly|cAMP-mediated signaling|protein dephosphorylation protein tyrosine phosphatase activity|protein tyrosine/serine/threonine phosphatase activity nucleus|preribosome, large subunit precursor|cytoplasm YLR063W_p YLR063W hom 0.0261581095042387 0.129928944486933 1264 Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cytoplasm YGR133W PEX4 hom FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION 0.0261494809297007 0.130055658835524 1265 Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis peroxisome organization|protein import into peroxisome matrix, receptor recycling|protein autoubiquitination|protein monoubiquitination|protein polyubiquitination ubiquitin-protein ligase activity peroxisome YPR197C_d YPR197C hom FF 0.0260972445987461 0.130824820026137 1266 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL060W TSR1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0260950378973389 0.130857390382522 1267 Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress rRNA processing|ribosome biogenesis ribonucleoprotein complex binding nucleolus|cytoplasm|90S preribosome YGR264C MES1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0260409964840587 0.131656991658808 1268 Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs methionyl-tRNA aminoacylation methionine-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|cytoplasm YMR192W GYL1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0259823274720262 0.132587137918261 1269 Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress ER to Golgi vesicle-mediated transport|regulation of exocytosis protein binding mitochondrion|incipient cellular bud site|cellular bud|cellular bud neck|cellular bud tip|cytoplasm|Golgi-associated vesicle|plasma membrane YIL046W MET30 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0259429900516088 0.133174658937237 1270 F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus response to cadmium ion|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|regulation of DNA-dependent DNA replication initiation|M phase of mitotic cell cycle|response to arsenic-containing substance|G1/S transition of mitotic cell cycle|protein polyubiquitination|regulation of transcription involved in G1 phase of mitotic cell cycle|protein ubiquitination ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus YLR457C NBP1 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0259192510267936 0.133472212802136 1271 Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication establishment of spindle pole body localization to nuclear envelope|spindle pole body duplication associated with nuclear envelope lipid binding integral to nuclear inner membrane|central plaque of spindle pole body|spindle pole body YCR089W FIG2 hom FT CELL WALL ORG/BIOGENESIS 0.0257181120091685 0.136630613553125 1272 Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating filamentous growth of a population of unicellular organisms|cell morphogenesis involved in conjugation with cellular fusion|invasive growth in response to glucose limitation|cytogamy molecular_function fungal-type cell wall|mating projection YDR293C SSD1 hom FF|FT CELL CYCLE|TRANSLATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|RNA LOCALIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0256464693032326 0.138967534410792 1273 Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function regulation of fungal-type cell wall organization|negative regulation of translation|intracellular mRNA localization mRNA binding|mRNA 5'-UTR binding|translation repressor activity, nucleic acid binding nucleus|cellular bud|cytoplasmic stress granule|cytoplasmic mRNA processing body|cellular bud neck|cytoplasm YDR449C UTP6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0255698666654266 0.138848010435453 1274 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YCR036W RBK1 hom FT CARBOHYDRATE METABOLISM|NUCLEUS 0.0255667776301651 0.138836794658793 1275 Putative ribokinase D-ribose metabolic process ribokinase activity|ATP binding nucleus|cytoplasm YMR124W_p YMR124W hom 0.0254961934220454 0.139930753432614 1276 Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization biological_process molecular_function cytoplasm YML052W SUR7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.0254712109958384 0.140319531363696 1277 Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants endocytosis|ascospore formation molecular_function cell cortex|eisosome|mitochondrion|membrane raft|integral to membrane|plasma membrane|actin cortical patch YLR118C YLR118C hom FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.0254654570658446 0.140409191901496 1278 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS protein deacylation palmitoyl-(protein) hydrolase activity nucleus|cytoplasm YJL058C BIT61 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0254372822039894 0.140848862289318 1279 Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|plasma membrane|cytoplasm YPR026W ATH1 hom FT CARBOHYDRATE METABOLISM 0.0254104648366093 0.141268330942707 1280 Acid trehalase required for utilization of extracellular trehalose trehalose catabolic process|response to stress alpha,alpha-trehalase activity fungal-type cell wall|cell wall-bounded periplasmic space|fungal-type vacuole YGR162W TIF4631 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION|RIBOSOME 0.0253656649132063 0.141971216758463 1281 Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication translational initiation|ribosomal large subunit biogenesis|stress granule assembly translation initiation factor activity mitochondrion|ribosome|eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule YDR135C YCF1 hom FT NUCLEOTIDE METABOLISM 0.0253344467272207 0.142462597875398 1282 Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR bilirubin transport|response to metal ion|transmembrane transport bilirubin transmembrane transporter activity|glutathione S-conjugate-exporting ATPase activity fungal-type vacuole membrane|integral to membrane YMR172W HOT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.025306575075507 0.143200976998495 1283 Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p cellular hyperosmotic salinity response|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YOL027C MDM38 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|TRANSLATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.025253354496467 0.143745105441438 1284 Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome potassium ion transport|mitochondrial respiratory chain complex III biogenesis|cellular potassium ion homeostasis|proton transport|positive regulation of mitochondrial translation|mitochondrial respiratory chain complex IV biogenesis|protein insertion into mitochondrial membrane ribosome binding mitochondrion|mitochondrial inner membrane YGR082W TOM20 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION|MITOCHONDRION 0.0252395143810882 0.143964874048186 1285 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins tRNA import into mitochondrion|protein import into mitochondrial matrix|mitochondrial outer membrane translocase complex assembly protein channel activity|mitochondrion targeting sequence binding mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YKR058W GLG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0251877595390753 0.144848954972461 1286 Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication glycogen biosynthetic process glycogenin glucosyltransferase activity cellular_component YDR505C PSP1 hom FT MITOCHONDRION 0.025181614631184 0.145367804190887 1287 Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication biological_process molecular_function mitochondrion|cytoplasm YOR020W-A_p YOR020W-A hom FT MITOCHONDRION 0.0251682408718703 0.145100713037709 1288 Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YJL164C TPK1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0251533933690851 0.145338189117269 1289 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication protein phosphorylation|Ras protein signal transduction cAMP-dependent protein kinase activity|protein kinase activity cAMP-dependent protein kinase complex|nucleus|cytoplasm YPL114W_d YPL114W hom 0.025150148225189 0.145390132664241 1290 Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Unknown Unknown Unknown YMR041C ARA2 hom FT OXIDATION-REDUCTION PROCESS 0.0250895745712857 0.146362317785524 1291 NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase dehydro-D-arabinono-1,4-lactone biosynthetic process D-arabinose 1-dehydrogenase [NAD(P)+] activity cellular_component YOR152C_p YOR152C hom 0.0250500050791177 0.147000073351218 1292 Putative protein of unknown function; YOR152C is not an essential gene biological_process molecular_function membrane YDR026C NSI1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0250191812913131 0.147558769092425 1293 RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication termination of RNA polymerase I transcription|chromatin silencing at rDNA|Unknown DNA binding ribosome|nucleolus|rDNA heterochromatin YDR278C_d YDR278C hom 0.0250149124516656 0.147567448983692 1294 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFR042W KEG1 het FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0250105048973787 0.147638828174705 1295 Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability chromosome organization|(1->6)-beta-D-glucan biosynthetic process molecular_function integral to endoplasmic reticulum membrane|integral to membrane YOL053W AIM39 hom FT MITOCHONDRION 0.0249831855255629 0.148081846810941 1296 Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YJR111C_p YJR111C hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0249824791280588 0.148093315401059 1297 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria biological_process molecular_function mitochondrion YMR186W HSC82 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION 0.0249703144679524 0.148290918987848 1298 Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication protein folding|proteasome assembly|protein refolding|telomere maintenance|'de novo' protein folding|box C/D snoRNP assembly|response to stress unfolded protein binding|ATPase activity, coupled|ATPase activity mitochondrion|plasma membrane|cytoplasm YMR281W GPI12 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0249528281303233 0.148878616634629 1299 ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp GPI anchor biosynthetic process N-acetylglucosaminylphosphatidylinositol deacetylase activity integral to membrane|endoplasmic reticulum membrane YER151C UBP3 hom FT VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS 0.024907999422931 0.149306327145695 1300 Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress protein deubiquitination|ribophagy|regulation of ER to Golgi vesicle-mediated transport|regulation of response to osmotic stress ubiquitin-specific protease activity|mRNA binding cytoplasm YPL067C_p YPL067C hom 0.0248857406390161 0.149670311287788 1301 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm YLR213C CRR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0248758879276996 0.149831642476611 1302 Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation ascospore wall assembly hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ascospore wall YER085C_p YER085C hom 0.0248387828593287 0.150501304956398 1303 Putative protein of unknown function biological_process molecular_function cellular_component YCL025C AGP1 hom FT PLASMA MEMBRANE 0.0248342106951817 0.150515544723586 1304 Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity|L-proline transmembrane transporter activity integral to membrane|plasma membrane YLR215C CDC123 het FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.0247900541426421 0.151303754309309 1305 Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein regulation of mitotic cell cycle|regulation of translation molecular_function cytoplasm YOR083W WHI5 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0247539244871585 0.151839702017852 1306 Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 negative regulation of transcription involved in G1/S phase of mitotic cell cycle|regulation of cell size RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription SBF transcription complex|nucleus|cytoplasm YDR257C RKM4 hom FT NUCLEUS 0.0247433785946219 0.15201429181253 1307 Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity nucleus YIL089W YIL089W hom FT ENDOPLASMIC RETICULUM 0.0247409482248888 0.152115707142667 1308 Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking biological_process molecular_function fungal-type vacuole lumen|integral to membrane|endoplasmic reticulum YKR057W RPS21A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0247347638967905 0.152157023550272 1309 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication cytoplasmic translation|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YMR123W PKR1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0247302198909291 0.153770398780745 1310 V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress vacuolar proton-transporting V-type ATPase complex assembly molecular_function integral to endoplasmic reticulum membrane YLL035W GRC3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0246844153279071 0.152993259068042 1311 Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated termination of RNA polymerase I transcription|rRNA processing polynucleotide 5'-hydroxyl-kinase activity nucleolar chromatin YMR194W RPL36A hom FT TRANSLATION|RIBOSOME 0.0246545493991389 0.153490948614924 1312 Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YIL019W FAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0246277914273482 0.153999342213158 1313 Protein required for pre-rRNA processing and 40S ribosomal subunit assembly maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|cytoplasm YIR008C PRI1 het FT NUCLEUS|CHROMOSOME 0.024627520356841 0.153942423801523 1314 Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA replication initiation|DNA replication single-stranded DNA binding|DNA primase activity alpha DNA polymerase:primase complex|nuclear replication fork YPL217C BMS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|NUCLEUS|MITOCHONDRION 0.024608378104144 0.154262773069642 1315 GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p ribosome assembly|rRNA processing U3 snoRNA binding|GTP binding|GTPase activity mitochondrion|nucleolus|nucleus|90S preribosome|cytoplasm YJR007W SUI2 het FF|FT TRANSLATION|RIBOSOME 0.0245797039980016 0.154928394778073 1316 Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress translational initiation translation initiation factor activity ribosome|multi-eIF complex|eukaryotic translation initiation factor 2 complex|cytoplasm YAL014C SYN8 hom FT PROTEIN LOCALIZATION 0.0245763382432491 0.154800098922096 1317 Endosomal SNARE related to mammalian syntaxin 8 transport SNAP receptor activity endosome YGL093W SPC105 het FF|FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0245346624816872 0.155624557133103 1318 Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components microtubule nucleation|protein localization to kinetochore|sister chromatid biorientation|mitotic cell cycle spindle assembly checkpoint microtubule binding|structural constituent of cytoskeleton mitochondrion|condensed nuclear chromosome kinetochore|spindle pole body YKL058W TOA2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0244808369203821 0.156471965625699 1319 TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|TBP-class protein binding RNA polymerase II transcription factor activity transcription factor TFIIA complex YNL245C CWC25 het FT RNA PROCESSING|NUCLEUS 0.0244688173799694 0.156613669241658 1320 Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YOR302W YOR302W hom FT TRANSLATION 0.0244589219097151 0.156781383535555 1321 CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation translation regulator activity cytosol YPL092W SSU1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0244346018329063 0.157566241175725 1322 Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein sulfite transport sulfite transmembrane transporter activity integral to membrane|plasma membrane YMR137C PSO2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.024395893887148 0.157852815823575 1323 Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress DNA repair|double-strand break repair damaged DNA binding|5'-3' exonuclease activity nucleus YNL067W-B_p YNL067W-B hom 0.0243638732884864 0.158399262949939 1324 Putative protein of unknown function biological_process molecular_function cellular_component YPL089C RLM1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|NUCLEUS 0.0243269681154869 0.159030842207455 1325 MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication signal transduction|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|DNA binding, bending|sequence-specific DNA binding nucleus YLL056C_p YLL056C hom 0.0243064352296082 0.159507622223672 1326 Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component YNR028W CPR8 hom 0.024300004594536 0.159493487211049 1327 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity fungal-type vacuole YLR109W AHP1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0242829179201665 0.159974256276558 1328 Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p cellular response to oxidative stress|response to metal ion|cell redox homeostasis thioredoxin peroxidase activity plasma membrane|cytoplasm YLL043W FPS1 hom FT ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0242663094332994 0.160073064462928 1329 Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress conjugation with cellular fusion|transport|arsenite transport|glycerol transport|acetate transport|response to arsenic-containing substance glycerol transmembrane transporter activity|transporter activity integral to membrane|plasma membrane|fungal-type vacuole|cytoplasm YER157W COG3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0242553847046177 0.160261317699956 1330 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|ER to Golgi vesicle-mediated transport|macroautophagy|retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway protein transporter activity Golgi transport complex YDL051W LHP1 hom FT RNA PROCESSING|NUCLEUS 0.0242315335843454 0.160672897732415 1331 RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding|tRNA binding nucleoplasm|nucleolus|nucleus YDL234C GYP7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM 0.0242240406932176 0.160802361327497 1332 GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking regulation of vacuole fusion, non-autophagic|vesicle-mediated transport Rab GTPase activator activity cytoplasm YML084W_d YML084W hom 0.0242234413897643 0.160812719607968 1333 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML100W TSL1 hom FT CARBOHYDRATE METABOLISM 0.0242217139659429 0.161155259582743 1334 Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication trehalose biosynthetic process|response to stress enzyme regulator activity|alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity|trehalose-phosphatase activity alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) YDR068W DOS2 hom 0.024186380672586 0.161454251632512 1335 Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YIR023W DAL81 hom FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0241715200718379 0.161712034949358 1336 Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism nitrogen utilization|nitrogen catabolite activation of transcription from RNA polymerase II promoter|gamma-aminobutyric acid catabolic process|urea catabolic process RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor recruiting transcription factor activity nucleus YNL092W_p YNL092W hom 0.0241608994439868 0.161896458687532 1337 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YMR037C MSN2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0241465720063426 0.162145501349218 1338 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|cellular response to methylmercury|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YOR106W VAM3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS 0.0241022417895254 0.162917889297499 1339 Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region autophagic vacuole fusion|vesicle fusion with vacuole|piecemeal microautophagy of nucleus|vacuole fusion, non-autophagic|vesicle docking|vesicle fusion protein homodimerization activity|SNAP receptor activity SNARE complex|fungal-type vacuole membrane YCL037C SRO9 hom FT TRANSLATION 0.0240847670507186 0.163223121581509 1340 Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication cytoplasmic translation RNA binding polysome YOR283W YOR283W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0240831657345329 0.163251113406678 1341 Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene dephosphorylation phosphatase activity nucleus|cytoplasm YKL006C-A SFT1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0240258174146509 0.164255977779094 1342 Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|Golgi membrane YKL052C ASK1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0240080244283039 0.164568693424271 1343 Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress attachment of spindle microtubules to kinetochore|attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization|mitotic sister chromatid segregation microtubule binding|structural constituent of cytoskeleton condensed nuclear chromosome kinetochore|DASH complex|condensed nuclear chromosome, centromeric region|mitotic spindle YMR036C MIH1 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0239857434152808 0.164960918847054 1344 Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity protein tyrosine phosphatase activity nucleus|cytoplasm YDR051C DET1 hom FT NUCLEUS 0.0239781306068288 0.165095092580649 1345 Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel intracellular sterol transport|dephosphorylation acid phosphatase activity nucleus|cytoplasm YDR089W YDR089W hom FT MEMBRANE ORGANIZATION 0.0239770096071314 0.165114856837465 1346 Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress cellular membrane organization molecular_function integral to membrane|membrane YHR041C SRB2 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RNA POL II, HOLOENZYME 0.023920599882192 0.166111712538366 1347 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus|RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|protein domain specific binding core mediator complex YLR232W_d YLR232W hom 0.0239066494114885 0.166358937442967 1348 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Unknown Unknown Unknown YLR103C CDC45 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0238998770934923 0.16647905353683 1349 DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes S phase of mitotic cell cycle|regulation of chromatin silencing at telomere|DNA replication initiation|pre-replicative complex assembly|double-strand break repair via break-induced replication DNA replication origin binding|chromatin binding replication fork protection complex|nucleus|DNA replication preinitiation complex|nuclear replication fork|pre-replicative complex YMR005W TAF4 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0238826252255734 0.166785332906493 1350 TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding transcription factor TFIID complex YPR083W MDM36 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0238822330378378 0.166855614651923 1351 Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission mitochondrial fission|mitochondrion organization molecular_function mitochondrion|cytoplasm YJR119C JHD2 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0238562685604704 0.167254070575696 1352 JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation histone demethylation histone demethylase activity (H3-K4 specific) nucleus|cytoplasm YEL011W GLC3 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0238035191356247 0.16819515816345 1353 Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress glycogen biosynthetic process 1,4-alpha-glucan branching enzyme activity cytoplasm YLR388W RPS29A hom FT TRANSLATION|RIBOSOME 0.0238018994769809 0.168224116783043 1354 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YNL276C_d YNL276C hom 0.0237776054154295 0.168658930483227 1355 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Unknown Unknown Unknown YLR321C SFH1 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.0237692100479315 0.168872971908934 1356 Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog ATP-dependent chromatin remodeling|double-strand break repair|chromosome segregation|regulation of DNA replication involved in S phase|chromatin remodeling at centromere|nucleosome disassembly|G2/M transition of mitotic cell cycle|transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YER133W GLC7 het FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CELL DIVISION|CARBOHYDRATE METABOLISM 0.0237582668783485 0.169196570882756 1357 Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest replication fork processing|glycogen metabolic process|chromosome segregation|regulation of cell shape during vegetative growth phase|termination of RNA polymerase II transcription, poly(A)-coupled|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|rRNA processing|regulation of cell cycle|ascospore formation|protein localization to kinetochore|cell budding|DNA damage checkpoint|meiosis|termination of RNA polymerase II transcription, exosome-dependent|DNA replication checkpoint|response to heat|cellular ion homeostasis|histone dephosphorylation|regulation of carbohydrate metabolic process protein serine/threonine phosphatase activity protein phosphatase type 1 complex|mating projection base|condensed nuclear chromosome kinetochore|mRNA cleavage and polyadenylation specificity factor complex|nucleolus|spindle pole body|cellular bud neck YOL078W AVO1 het FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0237247302818111 0.169608199766227 1358 Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth TOR signaling cascade|fungal-type cell wall organization|regulation of cell growth|negative regulation of Ras protein signal transduction|establishment or maintenance of actin cytoskeleton polarity phosphatidylinositol-4,5-bisphosphate binding|Ras GTPase binding TORC2 complex|plasma membrane|cytoplasm YCR105W ADH7 hom FT OXIDATION-REDUCTION PROCESS 0.0236943669776815 0.170155120214894 1359 NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance alcohol metabolic process alcohol dehydrogenase (NADP+) activity cellular_component YFL027C GYP8 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM 0.0236829885443144 0.170360414739705 1360 GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport vesicle-mediated transport Rab GTPase activator activity intracellular YMR059W SEN15 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0236826864292604 0.170365868154911 1361 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity tRNA-intron endonuclease complex YMR149W SWP1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0236427982465777 0.17108703012441 1362 Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YIL118W RHO3 het FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.023563353712121 0.172530158251517 1363 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p positive regulation of formin-nucleated actin cable assembly|establishment or maintenance of actin cytoskeleton polarity|positive regulation of exocytosis GTP binding|GTPase activity cytosol|plasma membrane|cellular bud YJL119C_d YJL119C hom 0.0235530635164325 0.172717745728988 1364 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR225W HTA1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.0235357604321396 0.173033519896594 1365 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair|chromatin assembly or disassembly|negative regulation of transcription from RNA polymerase II promoter DNA binding replication fork protection complex|nuclear nucleosome YDR126W SWF1 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|CYTOSKELETON 0.0235081559334004 0.173538183444498 1366 Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion ascospore wall assembly|cortical actin cytoskeleton organization|establishment of cell polarity|vacuole fusion, non-autophagic|regulation of exocytosis|protein palmitoylation palmitoyltransferase activity integral to membrane|actin filament bundle|nuclear outer membrane-endoplasmic reticulum membrane network|actin cortical patch YOR269W PAC1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CYTOSKELETON 0.0234686863498928 0.174261672267215 1367 Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly nuclear migration along microtubule microtubule plus-end binding cytoplasmic microtubule|nucleus|cytoplasm YBL053W_d YBL053W hom 0.0234555062609841 0.1745037678539 1368 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR180C CTL1 hom FT RNA PROCESSING|NUCLEUS 0.0234210830739323 0.175137246404594 1369 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm biological_process polynucleotide 5'-phosphatase activity nucleus|cytoplasm YDR294C DPL1 hom FT KETONE METABOLISM|LIPID METABOLISM|SIGNALING|RESPONSE TO NUTRIENT LEVELS|ENDOPLASMIC RETICULUM 0.0233816115948011 0.175930230272861 1370 Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate calcium-mediated signaling|sphingolipid metabolic process|cellular response to starvation sphinganine-1-phosphate aldolase activity endoplasmic reticulum|cortical endoplasmic reticulum|perinuclear endoplasmic reticulum YDR174W HMO1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0233802996256543 0.17621274691594 1371 Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase regulation of transcription from RNA polymerase I promoter|regulation of transcription by chromatin organization|regulation of ribosomal protein gene transcription from RNA polymerase II promoter double-stranded DNA binding|four-way junction DNA binding|DNA binding, bending nuclear chromatin|nucleolus|cytoplasm YER081W SER3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0233781021807984 0.176188787589532 1372 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication serine family amino acid biosynthetic process phosphoglycerate dehydrogenase activity cytoplasm YGR104C SRB5 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0233581849900132 0.176299166906304 1373 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly core mediator complex YBR033W_p EDS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0233127135455137 0.177142734409577 1374 Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication biological_process sequence-specific DNA binding cellular_component YDR195W REF2 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.023287375646449 0.177614094715887 1375 RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes mRNA 3'-end processing|termination of RNA polymerase II transcription, poly(A)-coupled|snoRNA 3'-end processing|Unknown chromatin binding|RNA binding mRNA cleavage and polyadenylation specificity factor complex|nucleus YDR309C GIC2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.023279100503192 0.178157015625485 1376 Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region regulation of exit from mitosis|establishment of cell polarity|axial cellular bud site selection|Rho protein signal transduction Rho GTPase binding|Rho GTPase activator activity|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|actin cap|plasma membrane|mating projection tip|cellular bud tip YLR343W GAS2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0232547216164806 0.179002130153289 1377 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p ascospore wall assembly 1,3-beta-glucanosyltransferase activity integral to membrane|cytoplasm YDR151C CTH1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 0.0231753421881192 0.179709443062727 1378 Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis nuclear-transcribed mRNA catabolic process|cellular iron ion homeostasis mRNA binding nucleus YHL002W HSE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0231638423655885 0.179925557806645 1379 Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT-0 complex YDL103C QRI1 het FT CARBOHYDRATE METABOLISM|NUCLEUS 0.023143311994406 0.180311862398542 1380 UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress UDP-N-acetylglucosamine biosynthetic process UDP-N-acetylglucosamine diphosphorylase activity nucleus|cytoplasm YGR224W AZR1 hom FT PLASMA MEMBRANE 0.023136798931167 0.180434542410943 1381 Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole azole transport azole transporter activity integral to membrane|plasma membrane YDL233W_p MFG1 hom FT NUCLEUS 0.0231186135132279 0.180777410885683 1382 Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene biological_process molecular_function nucleus|cytoplasm YDR363W-A SEM1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.0230620826366208 0.181846333930214 1383 Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|regulation of cell cycle|mRNA export from nucleus|exocytosis|filamentous growth|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|proteasome storage granule|proteasome regulatory particle, lid subcomplex YMR204C INP1 hom FT PEROXISOME ORGANIZATION 0.0230525770015507 0.182091767491024 1384 Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane peroxisome inheritance molecular_function peroxisome|extrinsic to intraperoxisomal membrane YHL041W_d YHL041W hom 0.0230390143191663 0.18228386882342 1385 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YOL107W_p YOL107W hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0230248276355621 0.182553333737176 1386 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein biological_process molecular_function COPI-coated vesicle|Golgi apparatus YGL123W RPS2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|NUCLEUS|RIBOSOME 0.0230022706537661 0.183047739794832 1387 Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 positive regulation of translational fidelity|rRNA export from nucleus structural constituent of ribosome|SSU rRNA binding small-subunit processome|cytosolic small ribosomal subunit YGL001C ERG26 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0230013466083589 0.182999985897161 1388 C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis ergosterol biosynthetic process C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity endoplasmic reticulum|endoplasmic reticulum membrane YLR090W XDJ1 hom FT NUCLEUS|MITOCHONDRION 0.0229856471414181 0.183299069787275 1389 Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding chaperone binding mitochondrion|integral to mitochondrial outer membrane|nucleus YDR282C_p YDR282C hom 0.0229556945763467 0.183870687342467 1390 Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation biological_process molecular_function cellular_component YGR168C_p YGR168C hom 0.0229337399940595 0.184290507963052 1391 Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function integral to membrane YML101C CUE4 hom FT ENDOPLASMIC RETICULUM 0.0229075828220845 0.184791616935179 1392 Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication biological_process molecular_function endoplasmic reticulum|cytoplasm YJR130C STR2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.022846034576006 0.186435078064763 1393 Cystathionine gamma-synthase, converts cysteine into cystathionine sulfur compound metabolic process|transsulfuration cystathionine gamma-synthase activity nucleus|cytoplasm YML043C RRN11 het FT NUCLEUS 0.022843286764452 0.186027658044791 1394 Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I CORE element sequence-specific DNA binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity RNA polymerase I core factor complex YLR435W TSR2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0228375980575016 0.186137312404336 1395 Protein with a potential role in pre-rRNA processing maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleus|cytoplasm YDR213W UPC2 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0227203709400359 0.188407600682188 1396 Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication cellular response to hypoxia|positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|membrane|cytoplasm|perinuclear region of cytoplasm YGL026C TRP5 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0227040207749958 0.188725862932521 1397 Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis tryptophan biosynthetic process tryptophan synthase activity nucleus|cytoplasm YDR275W BSC2 hom 0.0226949786702131 0.188902041070786 1398 Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function lipid particle YMR052W FAR3 hom FT CELL CYCLE|SIGNALING|ENDOPLASMIC RETICULUM 0.0226771714919788 0.189249353969836 1399 Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress re-entry into mitotic cell cycle after pheromone arrest molecular_function endoplasmic reticulum YOL083W ATG34 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS 0.0226073539763274 0.190814277458143 1400 Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog protein complex assembly|protein complex localization protein binding, bridging CVT complex YJL019W MPS3 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0225537828591663 0.191735174212286 1401 Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p meiotic telomere clustering|synapsis|nuclear migration involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope|telomere tethering at nuclear periphery|mitotic sister chromatid cohesion molecular_function nuclear envelope|nuclear periphery|integral to membrane|nuclear chromosome, telomeric region|half bridge of spindle pole body YIR006C PAN1 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0225499166572117 0.191745048370497 1402 Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease bipolar cellular bud site selection|endocytosis|axial cellular bud site selection|cytokinesis|actin cortical patch assembly protein binding, bridging nucleus|plasma membrane|mating projection tip|cytoplasm|actin cortical patch YCL057W PRD1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0225498853191112 0.191745665932327 1403 Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress proteolysis metalloendopeptidase activity mitochondrion|cytoplasm|mitochondrial intermembrane space YBR258C SHG1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0225471577538774 0.191799422086036 1404 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres histone H3-K4 methylation histone methyltransferase activity (H3-K4 specific) nucleus|Set1C/COMPASS complex YGL023C PIB2 hom FT VESICLE-MEDIATED TRANSPORT|MITOCHONDRION 0.0225260152828767 0.192548556664282 1405 Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 biological_process molecular_function mitochondrion YDR358W GGA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0225225734622457 0.192284441043452 1406 Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Golgi to vacuole transport|protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to endosome transport ubiquitin binding trans-Golgi network YKL162C_p YKL162C hom FT MITOCHONDRION 0.0225220087397424 0.192295592929365 1407 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion YDL075W RPL31A hom FT TRANSLATION|RIBOSOME 0.0225165960809085 0.192402503789698 1408 Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YER015W FAA2 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0224995397499719 0.192739686157763 1409 Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome long-chain fatty acid metabolic process long-chain fatty acid-CoA ligase activity|very long-chain fatty acid-CoA ligase activity|medium-chain fatty acid-CoA ligase activity mitochondrion|peroxisome YBR204C LDH1 hom FT LIPID METABOLISM 0.0224911336238937 0.192906024361375 1410 Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) lipid homeostasis hydrolase activity, acting on ester bonds|serine hydrolase activity|triglyceride lipase activity lipid particle YER132C PMD1 hom FT CELL CYCLE 0.0224773725793352 0.193178551806506 1411 Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions sporulation resulting in formation of a cellular spore molecular_function cytoplasm YPL071C_p YPL071C hom FT NUCLEUS 0.0224151886598814 0.19441358449746 1412 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YER109C FLO8 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0223756889645108 0.195201088553686 1413 Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YLR281C_p YLR281C hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION 0.0223638541197564 0.195437494045999 1414 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene biological_process molecular_function mitochondrion YJR005W APL1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE 0.0223626888116132 0.195460782857861 1415 Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex vesicle-mediated transport molecular_function AP-2 adaptor complex YNL265C IST1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0223626133269014 0.195462291498287 1416 Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p late endosome to vacuole transport via multivesicular body sorting pathway molecular_function endosome|cytoplasm YMR025W CSI1 hom FT SIGNALING|NUCLEUS 0.0223625634660514 0.195463288023881 1417 Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YBR210W ERV15 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION 0.0223373894692 0.19603363955569 1418 Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle YDR056C_p YDR056C hom FT ENDOPLASMIC RETICULUM 0.0222166336377003 0.198395835377375 1419 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein biological_process molecular_function endoplasmic reticulum YCR106W RDS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0222066148138112 0.198598343077735 1420 Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide response to xenobiotic stimulus sequence-specific DNA binding|DNA binding nucleus YDR339C FCF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0221935138536447 0.198863377001474 1421 Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YER040W GLN3 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0221854471736305 0.199026695354955 1422 Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source nitrogen catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity cytosol|nucleus YKR044W UIP5 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.0221680487820748 0.199379277472067 1423 Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates biological_process molecular_function nuclear envelope YBR249C ARO4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0221633887250437 0.199473791927347 1424 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity nucleus|cytoplasm YPL126W NAN1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0221582770774884 0.199577503084741 1425 U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|RENT complex|90S preribosome YFL052W_p YFL052W hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0221581842391241 0.199579387063359 1426 Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity biological_process DNA binding cellular_component YKL189W HYM1 het FT CELL DIVISION|SITE OF POLARIZED GROWTH 0.0221492284633064 0.199761188609676 1427 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response cytokinesis, completion of separation|regulation of cell shape|regulation of transcription, DNA-dependent|budding cell apical bud growth molecular_function incipient cellular bud site|intracellular|cellular bud|mating projection tip YOL082W ATG19 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0221100617000811 0.201028604716081 1428 Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles vesicle organization|ER-associated protein catabolic process|protein processing|protein complex localization|CVT pathway protein binding, bridging pre-autophagosomal structure|cytoplasm|CVT complex|extrinsic to membrane YOL158C ENB1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0220379875336786 0.202029455855403 1429 Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment ferric-enterobactin transport ferric-enterobactin transmembrane transporter activity integral to membrane|cellular bud membrane|plasma membrane YFL005W SEC4 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|PLASMA MEMBRANE|CYTOSKELETON 0.0220376456000479 0.202036456892346 1430 Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane bipolar cellular bud site selection|autophagy|exocytosis|Golgi to plasma membrane transport|small GTPase mediated signal transduction|ascospore-type prospore assembly|membrane addition at site of cytokinesis|vesicle fusion|cytokinesis GTP binding|GTPase activity mitochondrial outer membrane|vesicle|incipient cellular bud site|mitochondrion|transport vesicle|actin cap|plasma membrane YAR008W SEN34 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0220181026869986 0.202436888760109 1431 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YOL145C CTR9 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|GENE SILENCING|NUCLEUS|CHROMOSOME|RNA POL II, HOLOENZYME 0.0219788601050576 0.203242709568055 1432 Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats transcription elongation from RNA polymerase I promoter|mRNA 3'-end processing|regulation of chromatin silencing at telomere|snoRNA transcription from an RNA polymerase II promoter|regulation of transcription by chromatin organization|transcription from RNA polymerase I promoter|regulation of histone H3-K4 methylation|regulation of transcription-coupled nucleotide-excision repair|regulation of transcription initiation from RNA polymerase II promoter|snoRNA 3'-end processing|Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter RNA polymerase II core binding|triplex DNA binding|RNA polymerase II transcription factor binding transcription factor activity transcriptionally active chromatin|nucleus|Cdc73/Paf1 complex YPL063W TIM50 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0219435091691649 0.203970614581351 1433 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel protein import into mitochondrial matrix mitochondrion targeting sequence binding|protein channel activity mitochondrion|mitochondrial inner membrane presequence translocase complex YOL147C PEX11 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|PEROXISOME ORGANIZATION|ENDOPLASMIC RETICULUM 0.0219311570727869 0.204563343985749 1434 Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p peroxisome fission|fatty acid oxidation molecular_function endoplasmic reticulum|peroxisomal membrane YLR011W LOT6 hom FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0219269667726592 0.204311886747634 1435 FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions cellular response to oxidative stress|cytoplasmic sequestering of transcription factor NAD(P)H dehydrogenase (quinone) activity|transcription factor binding cytosol|nucleus|cytoplasm YEL045C_d YEL045C hom 0.021865789356386 0.205577593146094 1436 Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Unknown Unknown Unknown YOR272W YTM1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0218320132537931 0.206278826186204 1437 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats chromosome organization|ribosomal large subunit biogenesis molecular_function PeBoW complex|nucleolus|nucleus|preribosome, large subunit precursor YGR143W SKN1 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0218285482926455 0.206350861080061 1438 Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p fungal-type cell wall organization|(1->6)-beta-D-glucan biosynthetic process|sphingolipid biosynthetic process glucosidase activity integral to membrane YKL046C DCW1 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0218198194246513 0.206532411027854 1439 Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p fungal-type cell wall biogenesis|budding cell bud growth mannosidase activity anchored to plasma membrane YML099C ARG81 hom FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0217118244562873 0.209128141274915 1440 Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p arginine metabolic process|negative regulation of calcium ion-dependent exocytosis transcription cofactor activity nucleus YHL006C SHU1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0216670550668128 0.209728512908007 1441 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function recombinational repair|maintenance of rDNA molecular_function nucleolus|Shu complex YGR008C STF2 hom FT MITOCHONDRION 0.0216603748544339 0.210073268763317 1442 Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress cellular response to desiccation molecular_function cytoplasm YER056C FCY2 hom FT PLASMA MEMBRANE 0.0216091809281548 0.2109486443062 1443 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress cytosine transport|cytidine transport|purine nucleobase transport|transmembrane transport nucleobase transmembrane transporter activity|cytidine transmembrane transporter activity integral to membrane|plasma membrane YNL242W ATG2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.0215960408278685 0.211226383841527 1444 Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress mitochondrion degradation|piecemeal microautophagy of nucleus|peroxisome degradation|CVT pathway|autophagic vacuole assembly molecular_function pre-autophagosomal structure|cytoplasm|extrinsic to membrane YKL180W RPL17A het FT TRANSLATION|RIBOSOME 0.0215416780755171 0.212378246474256 1445 Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|cytoplasm YLR246W ERF2 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0214739981002182 0.214366247063595 1446 Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole protein targeting to membrane|protein palmitoylation palmitoyltransferase activity endoplasmic reticulum palmitoyltransferase complex|integral to endoplasmic reticulum membrane|integral to membrane YOR355W GDS1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0214708716966659 0.21388531534148 1447 Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies aerobic respiration molecular_function mitochondrion|nucleus|cytoplasm YDL049C KNH1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.021400113287473 0.215467542617306 1448 Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance (1->6)-beta-D-glucan biosynthetic process molecular_function fungal-type cell wall|extracellular region YDR170C SEC7 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0213951247137711 0.215506062643105 1449 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|autophagic vacuole assembly ARF guanyl-nucleotide exchange factor activity trans-Golgi network|cytosol|late endosome|integral to membrane|Golgi-associated vesicle YLR026C SED5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0213612409800569 0.21630248093616 1450 cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|cis-Golgi network YIL109C SEC24 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.021328559492683 0.217074884420542 1451 Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat YGL134W PCL10 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0213265197864833 0.216981607583051 1452 Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate negative regulation of glycogen biosynthetic process|regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YJL131C AIM23 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0213207055410053 0.217106992675601 1453 Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss positive regulation of mitochondrial translational initiation ribosome binding|translation initiation factor activity mitochondrion YLR420W URA4 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0212701710487569 0.218198971334941 1454 Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine nucleotide biosynthetic process dihydroorotase activity nucleus|cytoplasm YDL218W_p YDL218W hom 0.0212568653617069 0.218487143379779 1455 Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin biological_process molecular_function integral to membrane|cytoplasm YKL212W SAC1 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0212111541065442 0.219479231226943 1456 Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity mitochondrial outer membrane|integral to endoplasmic reticulum membrane|mitochondrion|SPOTS complex|integral to Golgi membrane YER149C PEA2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.021196205835448 0.219804359205175 1457 Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth bipolar cellular bud site selection|regulation of initiation of mating projection growth|regulation of termination of mating projection growth|actin filament organization|pseudohyphal growth|establishment of cell polarity|filamentous growth|Rho protein signal transduction cytoskeletal regulatory protein binding polarisome|actin cap|mating projection tip YLR440C SEC39 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0211631041489105 0.220525556955372 1458 Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope retrograde vesicle-mediated transport, Golgi to ER|vesicle-mediated transport|ER-dependent peroxisome organization molecular_function Dsl1p complex|nuclear envelope|endoplasmic reticulum|peroxisome|endoplasmic reticulum membrane YMR193C-A_d YMR193C-A hom 0.0211381349310338 0.221070691099465 1459 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL002C OST1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0211299110127576 0.221250448920782 1460 Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation via asparagine|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YJR013W GPI14 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0211221338942645 0.22142053693555 1461 Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M fungal-type cell wall organization|GPI anchor biosynthetic process mannosyltransferase activity integral to membrane|endoplasmic reticulum membrane YNL237W YTP1 hom FF|FT MITOCHONDRION 0.0210585243658895 0.222815213789001 1462 Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins biological_process molecular_function integral to membrane|membrane YNL091W NST1 hom 0.0210099577731996 0.223884288290071 1463 Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 response to salt stress molecular_function cytoplasm YKL018C-A_p YKL018C-A hom 0.0209950242233107 0.224213749558125 1464 Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YHR127W YHR127W hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS 0.020983424410841 0.224469901459357 1465 Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis mitotic spindle elongation molecular_function nucleus YMR308C PSE1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RNA LOCALIZATION|NUCLEUS 0.0208941480179705 0.226448337724501 1466 Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p mRNA export from nucleus|regulation of protein desumoylation|protein import into nucleus|regulation of mitosis protein transporter activity|nuclear localization sequence binding integral to membrane|nucleus|cytoplasm YJR056C YJR056C hom FT NUCLEUS 0.0208863641885845 0.226621421119337 1467 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YNL181W_p YNL181W het FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.020874043206561 0.227034299476839 1468 Putative oxidoreductase; required for cell viability biological_process oxidoreductase activity|oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor nuclear envelope|endoplasmic reticulum YDR285W ZIP1 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.020839128248226 0.227673795806426 1469 Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate synapsis|reciprocal meiotic recombination chromatin binding|SUMO polymer binding transverse filament YEL012W UBC8 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS 0.0208232763685544 0.228027740492404 1470 Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YDR037W KRS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.020806581310477 0.228400935339682 1471 Lysyl-tRNA synthetase lysyl-tRNA aminoacylation lysine-tRNA ligase activity cytoplasm YKL116C PRR1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN PHOSPHORYLATION 0.02079619259688 0.22863337995445 1472 Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor negative regulation of transcription from RNA polymerase II promoter by pheromones|negative regulation of conjugation with cellular fusion|protein phosphorylation protein kinase activity|receptor signaling protein serine/threonine kinase activity cytoplasm YLL034C RIX7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0207569306360127 0.229513375953493 1473 Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus ATPase activity nucleolus|nucleus|preribosome, large subunit precursor YKR064W OAF3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0207506183914506 0.230560945324814 1474 Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|cellular response to oleic acid RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity mitochondrion|nucleus|cytoplasm YDR271C_d YDR271C hom 0.0207388563514595 0.229919291680335 1475 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Unknown Unknown Unknown YDL085W NDE2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0207222289743602 0.230501953622349 1476 Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain glucose catabolic process to ethanol|NADH oxidation NADH dehydrogenase activity mitochondrion YGR063C SPT4 hom FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0206978289388547 0.230842586558495 1477 Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions 7-methylguanosine mRNA capping|chromosome segregation|chromatin organization|intracellular mRNA localization|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription, DNA-dependent|regulation of transcription-coupled nucleotide-excision repair|chromatin silencing|mRNA splicing, via spliceosome|Unknown core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rDNA binding DSIF complex|nuclear telomeric heterochromatin|rDNA heterochromatin|mating-type region heterochromatin|kinetochore YLR384C IKI3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0206933345153335 0.230943890429093 1478 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification molecular_function Elongator holoenzyme complex|nucleus|cytoplasm YPL030W TRM44 hom FT RNA PROCESSING 0.0206891890488214 0.231106976874324 1479 tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene tRNA methylation tRNA (uracil) methyltransferase activity cytoplasm YMR301C ATM1 het FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0206678158808699 0.231589327795848 1480 Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol cellular iron ion homeostasis ATPase activity, coupled to transmembrane movement of substances|ATPase activity mitochondrion|integral to membrane|mitochondrial inner membrane YMR020W FMS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.0206592524714869 0.231713124077068 1481 Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis pantothenate biosynthetic process|polyamine catabolic process polyamine oxidase activity cytoplasm YNR048W YNR048W hom 0.0206505675884169 0.231909432796254 1482 Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication response to drug phospholipid-translocating ATPase activity integral to membrane YNL278W CAF120 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|SITE OF POLARIZED GROWTH 0.020627632038376 0.23242842372848 1483 Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter molecular_function CCR4-NOT complex YMR238W DFG5 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0206224963877816 0.232544747220406 1484 Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p pseudohyphal growth|fungal-type cell wall biogenesis|budding cell bud growth mannosidase activity anchored to plasma membrane YDR146C SWI5 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0205911961017048 0.233254597552835 1485 Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication positive regulation of mating type switching|regulation of transcription involved in G1 phase of mitotic cell cycle|positive regulation of transcription on exit from mitosis, from RNA polymerase II promoter RNA polymerase II distal enhancer sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity|mediator complex binding nucleus|cytoplasm YDR292C SRP101 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0205902046696116 0.233277107027815 1486 Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p SRP-dependent cotranslational protein targeting to membrane|protein targeting to ER GTP binding|signal recognition particle binding endoplasmic reticulum membrane|signal recognition particle receptor complex YBR129C OPY1 hom FT MITOCHONDRION 0.0204332819437787 0.236859307865538 1487 Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YLR389C STE23 hom FT PROTEOLYSIS 0.0203943831083461 0.237753262270852 1488 Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family peptide pheromone maturation metallopeptidase activity|metalloendopeptidase activity extrinsic to membrane YOR081C TGL5 hom FT LIPID METABOLISM 0.0203633703400236 0.238677992250668 1489 Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate triglyceride mobilization lysophosphatidic acid acyltransferase activity|triglyceride lipase activity lipid particle YDR427W RPN9 het FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS 0.0203519274823152 0.238731672690294 1490 Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress proteasome assembly|ubiquitin-dependent protein catabolic process structural molecule activity proteasome storage granule|proteasome regulatory particle, lid subcomplex YNR024W MPP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0203365072393535 0.239298154156884 1491 Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes nuclear mRNA surveillance of spliceosomal pre-mRNA splicing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding ribosome|nucleus|nuclear exosome (RNase complex)|TRAMP complex YOR345C_d YOR345C hom 0.0203100717169957 0.239699035703749 1492 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YLR229C CDC42 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0202927999913312 0.24009902081644 1493 Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins budding cell apical bud growth|Rho protein signal transduction|budding cell isotropic bud growth|regulation of initiation of mating projection growth|regulation of vacuole fusion, non-autophagic|regulation of exit from mitosis|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|establishment of cell polarity|invasive growth in response to glucose limitation|positive regulation of exocytosis GTPase activity|signal transducer activity nuclear membrane|fungal-type vacuole membrane|incipient cellular bud site|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YMR074C YMR074C hom FT NUCLEUS 0.0202774980750184 0.240453780967173 1494 Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress apoptotic process molecular_function nucleus|cytoplasm YDL203C ACK1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0202307780434036 0.241539223406092 1495 Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria fungal-type cell wall organization|positive regulation of signal transduction molecular_function mitochondrion YGL114W_p YGL114W hom FT PROTEIN LOCALIZATION 0.0202259225678733 0.241652227685756 1496 Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters transport oligopeptide transporter activity integral to membrane|membrane YKL030W_d YKL030W hom 0.0202097282503175 0.242029396241541 1497 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1 Unknown Unknown Unknown YML123C PHO84 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0201951572677954 0.242439407402117 1498 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity phosphate ion transport|manganese ion transport|polyphosphate metabolic process manganese ion transmembrane transporter activity|inorganic phosphate transmembrane transporter activity integral to membrane|integral to plasma membrane YJL208C NUC1 hom FT NUCLEUS|MITOCHONDRION 0.0201702615936639 0.2429503150521 1499 Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy DNA catabolic process|RNA catabolic process|apoptotic process|DNA recombination ribonuclease activity|endodeoxyribonuclease activity|exodeoxyribonuclease activity mitochondrion|mitochondrial inner membrane|nucleus YDR307W_p PMT7 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0201325359994602 0.243832906793785 1500 Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation biological_process molecular_function integral to membrane|endoplasmic reticulum YBR297W MAL33 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0201243169551372 0.244025489929646 1501 MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C cellular carbohydrate metabolic process|regulation of transcription, DNA-dependent sequence-specific DNA binding transcription factor activity nucleus YKL141W SDH3 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0201034122100855 0.244515796987622 1502 Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex mitochondrial electron transport, succinate to ubiquinone|cellular respiration|tricarboxylic acid cycle|protein import into mitochondrial inner membrane succinate dehydrogenase (ubiquinone) activity mitochondrial respiratory chain complex II|mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YER069W ARG5,6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0200922058157313 0.24477892002069 1503 Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine ornithine biosynthetic process|arginine biosynthetic process|regulation of transcription, DNA-dependent N-acetyl-gamma-glutamyl-phosphate reductase activity|acetylglutamate kinase activity mitochondrion|mitochondrial matrix YHR005C-A TIM10 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0200907816148797 0.244812374082413 1504 Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity|unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex|mitochondrial intermembrane space protein transporter complex YGR119C NUP57 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0200845588659764 0.244958582256998 1505 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) NLS-bearing substrate import into nucleus|protein import into nucleus|nuclear pore organization|protein targeting to nuclear inner membrane nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YJR019C TES1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0200697430601745 0.246083289436197 1506 Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids fatty acid beta-oxidation|fatty acid oxidation acyl-CoA hydrolase activity mitochondrion|peroxisome YOL163W_p YOL163W hom 0.0200393571478044 0.246022469027017 1507 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YML013W UBX2 hom FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.0200366370143776 0.246086594362866 1508 Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis proteasomal ubiquitin-dependent protein catabolic process|ER-associated protein catabolic process|lipid particle organization protein binding, bridging mitochondrial outer membrane|Doa10p ubiquitin ligase complex|integral to endoplasmic reticulum membrane|lipid particle|endoplasmic reticulum YKL094W YJU3 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0200133760107728 0.246635435348201 1509 Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family triglyceride metabolic process serine hydrolase activity|acylglycerol lipase activity mitochondrial outer membrane|lipid particle|plasma membrane|membrane|cytoplasm YNL144C_p YNL144C hom FT MITOCHONDRION 0.0200000627305939 0.246949946277479 1510 Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study biological_process molecular_function mitochondrion YDL208W NHP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.01998706691361 0.247257228238337 1511 Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing cleavage involved in rRNA processing|rRNA pseudouridine synthesis|snRNA pseudouridine synthesis snoRNA binding box H/ACA snoRNP complex YLR406C RPL31B hom FT TRANSLATION|RIBOSOME 0.019955322447082 0.248220789734598 1512 Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YIR012W SQT1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RIBOSOME 0.0199418703424956 0.24832797022974 1513 Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress ribosomal large subunit assembly molecular_function cytosol|cytosolic ribosome|cytoplasm YGR005C TFG2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0199375480626195 0.248430538015388 1514 TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 transcriptional start site selection at RNA polymerase II promoter|transcription initiation from RNA polymerase II promoter|promoter clearance from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|core RNA polymerase II recruiting transcription factor activity transcription factor TFIIF complex YHR193C EGD2 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.0199181478692137 0.248961905141018 1515 Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes 'de novo' cotranslational protein folding unfolded protein binding|phosphatidic acid binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding nascent polypeptide-associated complex YMR277W FCP1 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0199166121614631 0.248998403680107 1516 Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) dephosphorylation of RNA polymerase II C-terminal domain|regulation of transcription from RNA polymerase II promoter protein serine/threonine phosphatase activity nucleus YGR146C-A_p YGR146C-A hom 0.0199150859030977 0.248964042442954 1517 Putative protein of unknown function biological_process molecular_function cellular_component YBR109C CMD1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0199077448954174 0.249138574045325 1518 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin karyogamy involved in conjugation with cellular fusion|phosphatidylinositol biosynthetic process|NLS-bearing substrate import into nucleus|microautophagy|cell budding|vacuole fusion, non-autophagic|receptor-mediated endocytosis|cytoskeleton organization|spindle pole body organization|transcription factor import into nucleus calcium-dependent protein binding|protein binding|calcium ion binding incipient cellular bud site|central plaque of spindle pole body|mating projection tip|cellular bud neck|cellular bud tip YBR082C UBC4 hom FT PROTEOLYSIS 0.0199077093776714 0.249139418683368 1519 Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein monoubiquitination|protein polyubiquitination|protein ubiquitination|response to stress ubiquitin binding|ubiquitin-protein ligase activity proteasome complex YNL159C ASI2 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0199052982164822 0.249196762543043 1520 Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals ubiquitin-dependent protein catabolic process molecular_function nuclear inner membrane YJL185C ATG36 hom 0.0198210982917151 0.251205048157042 1521 Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene peroxisome degradation molecular_function peroxisome YDR191W HST4 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.019820645963939 0.251215867200776 1522 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|chromatin silencing at telomere|histone deacetylation histone deacetylase activity|DNA binding nucleus|cytoplasm YDL021W GPM2 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0198187784799822 0.251260538229007 1523 Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process phosphoglycerate mutase activity|molecular_function cytosol|cytoplasm YIL098C FMC1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0197901603194183 0.251945790063236 1524 Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p mitochondrial proton-transporting ATP synthase complex assembly molecular_function mitochondrion|mitochondrial matrix YJR069C HAM1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0197711999887829 0.252400505278236 1525 Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress purine deoxyribonucleoside triphosphate catabolic process|pyrimidine deoxyribonucleoside triphosphate catabolic process dITP diphosphatase activity|ITP diphosphatase activity|XTP diphosphatase activity|dCTP diphosphatase activity|UTP diphosphatase activity|dTTP diphosphatase activity|dATP pyrophosphohydrolase activity|dGTP diphosphatase activity|dUTP diphosphatase activity|GTP diphosphatase activity nucleus|cytoplasm YLR197W NOP56 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0197673509813474 0.252847162659339 1526 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects rRNA modification|rRNA processing molecular_function box C/D snoRNP complex|small-subunit processome|nucleolus|nucleus|90S preribosome YLR364W GRX8 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.019760472034012 0.252658041062676 1527 Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic biological_process glutathione-disulfide reductase activity cytoplasm YNR031C SSK2 hom FT SIGNALING|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.0197510539860702 0.252884281987612 1528 MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress MAPK cascade involved in osmosensory signaling pathway|protein autophosphorylation|protein phosphorylation|response to osmotic stress|actin cytoskeleton organization|osmosensory signaling pathway via two-component system MAP kinase kinase kinase activity cytosol|cellular bud neck|cellular bud tip YLR423C ATG17 hom FT MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.0197021928437234 0.254060291757403 1529 Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells piecemeal microautophagy of nucleus|mitochondrion degradation|peroxisome degradation|activation of protein kinase activity|autophagic vacuole assembly protein kinase activator activity|protein complex scaffold pre-autophagosomal structure|Atg1p signaling complex YAL046C AIM1 hom 0.0196868383978315 0.254501527998744 1530 Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YML006C GIS4 hom FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0196696739938996 0.254845074001121 1531 CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway cellular ion homeostasis|intracellular signal transduction molecular_function plasma membrane YLR272C YCS4 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0196528440707799 0.255251894121263 1532 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation maintenance of rDNA|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation|chromatin silencing at silent mating-type cassette molecular_function nucleolus|nuclear condensin complex YHR026W VMA16 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0196262203937209 0.256109324997291 1533 Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane YIL129C TAO3 het FT CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0196026863078462 0.25646700482057 1534 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p cell morphogenesis|cell budding|budding cell apical bud growth molecular_function incipient cellular bud site|mitochondrion|cellular bud|mating projection tip YGL101W_p YGL101W hom FT NUCLEUS 0.0195278288961221 0.258287941450846 1535 Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p biological_process molecular_function nucleus|cytoplasm YCR071C IMG2 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.019496726847252 0.259474053627127 1536 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YNR005C_d YNR005C hom 0.0194444446615856 0.26032682215177 1537 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR013W PSF1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0194295577211925 0.260692000115391 1538 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YDL031W DBP10 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.01941635772689 0.261016093281819 1539 Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus|preribosome, large subunit precursor YGL086W MAD1 hom FT CELL CYCLE|CELL DIVISION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|NUCLEAR PORE|KINETOCHORE 0.0193714227177496 0.262121448430092 1540 Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p nucleocytoplasmic transport|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function nucleus|nuclear pore|kinetochore YLR294C_d YLR294C hom 0.0193640486246438 0.262303151859254 1541 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Unknown Unknown Unknown YIL159W BNR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0193411733398751 0.262867370032678 1542 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 barbed-end actin filament capping|actin filament bundle assembly|formin-nucleated actin cable assembly profilin binding cellular bud neck YNL231C PDR16 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0193267829583135 0.263507903407456 1543 Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress response to drug|sterol biosynthetic process|phospholipid biosynthetic process|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cell periphery|lipid particle|plasma membrane|cytoplasm YOR054C VHS3 hom FT CELL CYCLE|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|COFACTOR METABOLISM 0.0193206147161681 0.263375161922008 1544 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis coenzyme A biosynthetic process|cellular monovalent inorganic cation homeostasis phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex YER079W_p YER079W hom FT NUCLEUS 0.0192302555760284 0.265615025377607 1545 Putative protein of unknown function biological_process molecular_function nucleus|cytoplasm YDR015C_d YDR015C hom 0.019230113063718 0.265618568362591 1546 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Unknown Unknown Unknown YKR039W GAP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PLASMA MEMBRANE 0.0192200367238486 0.265869157753732 1547 General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth amino acid transport|gamma-aminobutyric acid transport|ammonia assimilation cycle|polyamine transport amino acid transmembrane transporter activity|polyamine transmembrane transporter activity|L-proline transmembrane transporter activity endosome|fungal-type vacuole lumen|integral to membrane|plasma membrane|ER to Golgi transport vesicle|integral to plasma membrane|multivesicular body YPR027C_p YPR027C hom 0.0192106556719409 0.266102602162811 1548 Putative protein of unknown function biological_process molecular_function cellular_component YMR260C TIF11 het FT TRANSLATION|RIBOSOME 0.0191961658951383 0.266463452589962 1549 Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 translational initiation double-stranded RNA binding|translation initiation factor activity ribosome YEL027W VMA3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.0191316637771831 0.268288240092031 1550 Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis cellular copper ion homeostasis|cellular iron ion homeostasis|endocytosis|vacuole organization|proton transport|vacuolar acidification|protein targeting to vacuole hydrogen ion transmembrane transporter activity vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane YKL163W PIR3 hom FT CELL WALL ORG/BIOGENESIS 0.0191170293671964 0.268440190097336 1551 O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall YGR011W_d YGR011W hom 0.0190881162118631 0.26916491154094 1552 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL151W NUT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0190628863317729 0.269798405818297 1553 Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription regulation of transcription from RNA polymerase II promoter|transcription from RNA polymerase II promoter molecular_function mitochondrion|mediator complex|nucleus YER086W ILV1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.019042578883009 0.270309045344327 1554 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation threonine catabolic process|isoleucine biosynthetic process L-threonine ammonia-lyase activity mitochondrion YOL062C APM4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE 0.0190415674557554 0.270405998474107 1555 Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport intracellular protein transport|vesicle-mediated transport molecular_function AP-2 adaptor complex YMR206W_p YMR206W hom 0.0190262191679645 0.270720898864201 1556 Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication biological_process molecular_function cellular_component YOR195W SLK19 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0190223136991242 0.270819282023592 1557 Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate meiosis|mitotic spindle organization in nucleus molecular_function spindle midzone|condensed nuclear chromosome kinetochore|kinetochore|spindle YDR046C BAP3 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0190064302115132 0.27121965731377 1558 Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YDR208W MSS4 het FT LIPID METABOLISM|NUCLEUS|PLASMA MEMBRANE 0.0189983755769129 0.271422845411545 1559 Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation phosphatidylinositol phosphorylation|ascospore-type prospore assembly 1-phosphatidylinositol-4-phosphate 5-kinase activity nucleus|plasma membrane YLR424W SPP382 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.018991921883424 0.271585722981554 1560 Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation spliceosomal complex disassembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step RNA binding|ATP-dependent RNA helicase activity mitochondrion|U2-type post-mRNA release spliceosomal complex|nucleus|spliceosomal complex|cytoplasm YMR142C RPL13B hom FT TRANSLATION|RIBOSOME 0.0189472578320152 0.272714782907485 1561 Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL241W_p YDL241W hom 0.0189067026412309 0.27374275195623 1562 Putative protein of unknown function; YDL241W is not an essential gene biological_process molecular_function cellular_component YPL080C_d YPL080C hom 0.0189050508035936 0.273784677794723 1563 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLL051C FRE6 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0189002593688936 0.273977928401207 1564 Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels intracellular sequestering of iron ion|copper ion import ferric-chelate reductase activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YKL127W PGM1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0188923503651271 0.274107178357291 1565 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism glucose 6-phosphate metabolic process|galactose catabolic process|glucose 1-phosphate metabolic process|UDP-glucose metabolic process|trehalose biosynthetic process|glycogen biosynthetic process phosphoglucomutase activity cytoplasm YBL042C FUI1 hom FF|FT PLASMA MEMBRANE 0.01888232323741 0.274361979097092 1566 High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress uridine transport|transmembrane transport uridine transmembrane transporter activity integral to membrane|plasma membrane YAL001C TFC3 het FF|FT PROTEIN LOCALIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0188711158820018 0.274646962106407 1567 Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding protein localization to chromatin|transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex|mitochondrion|nuclear condensin complex YLL011W SOF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0188650728748177 0.274800708794833 1568 Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YDR095C_d YDR095C hom 0.0188070644234286 0.276781807558763 1569 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL211W NCS6 hom FT RNA PROCESSING|MITOCHONDRION 0.0187907861173806 0.276695518395247 1570 Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA wobble position uridine thiolation|protein urmylation|tRNA wobble uridine modification tRNA binding cytosol|mitochondrion YKL001C MET14 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0187889865727441 0.276741528988939 1571 Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process adenylylsulfate kinase activity cytoplasm YER057C HMF1 hom FT NUCLEUS 0.018747988177354 0.277791184467464 1572 Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro biological_process molecular_function cytosol|nucleus|cytoplasm YOL022C TSR4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS 0.0187463717174766 0.277832625097571 1573 Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 maturation of SSU-rRNA molecular_function cytoplasm YER105C NUP157 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0187414191598068 0.277959618320284 1574 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication nucleocytoplasmic transport|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|mitotic cell cycle spindle assembly checkpoint|nuclear pore complex assembly|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YDR450W RPS18A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|MITOCHONDRION|RIBOSOME 0.0187171406616457 0.278582737704814 1575 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit|mitochondrion YIL079C AIR1 hom FT RNA PROCESSING|NUCLEUS 0.0187164052619516 0.278601626905804 1576 Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent snRNA catabolic process polynucleotide adenylyltransferase activity nucleolus|TRAMP complex YBR188C NTC20 hom FT RNA PROCESSING|NUCLEUS 0.0187085227037619 0.278804150048652 1577 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YDR274C_d YDR274C hom 0.0186583126065872 0.280599477675624 1578 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR097W YPR097W hom FT MITOCHONDRION 0.0186342251494658 0.280717963186746 1579 Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process phosphatidylinositol binding mitochondrion YNL149C PGA2 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0186197090685075 0.281380277281089 1580 Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect protein transport molecular_function nuclear envelope|endoplasmic reticulum YIL162W SUC2 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0186048206175022 0.281477844684605 1581 Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively sucrose catabolic process beta-fructofuranosidase activity mitochondrion|extracellular region|cytoplasm YDR525W-A SNA2 hom 0.0186000202169466 0.281602030550929 1582 Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function fungal-type vacuole membrane|integral to membrane|cytoplasm YAL026C DRS2 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|GOLGI APPARATUS 0.0185815755856323 0.282151365328538 1583 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease intracellular protein transport|post-Golgi vesicle-mediated transport|phospholipid translocation|endocytosis|ribosomal small subunit assembly phospholipid-translocating ATPase activity trans-Golgi network|integral to membrane YLR175W CBF5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0185468763553954 0.282979339496437 1584 Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita box H/ACA snoRNA 3'-end processing|rRNA pseudouridine synthesis|rRNA processing|snRNA pseudouridine synthesis pseudouridine synthase activity box H/ACA snoRNP complex|nucleolus|90S preribosome YDL164C CDC9 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0185135209944397 0.283846124289271 1585 DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination lagging strand elongation|base-excision repair|mitotic cell cycle|DNA ligation|DNA recombination|nucleotide-excision repair DNA ligase (ATP) activity mitochondrion|nucleus|replication fork YLR078C BOS1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0184670735111097 0.285056116125145 1586 v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|ER to Golgi transport vesicle membrane|endoplasmic reticulum membrane YJR079W_p YJR079W hom 0.0184615985842273 0.285991115636769 1587 Putative protein of unknown function; mutation results in impaired mitochondrial respiration biological_process molecular_function cellular_component YPL248C GAL4 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0184527476105064 0.285430019145406 1588 DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p galactose metabolic process|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus YPR187W RPO26 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0184520257876788 0.285448867336951 1589 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YKL164C PIR1 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS 0.0184360758856358 0.285865564785163 1590 O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication intracellular protein transport|fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall YMR060C SAM37 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0184075498201701 0.286611843575382 1591 Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion|integral to membrane YEL016C NPP2 hom FT NUCLEOTIDE METABOLISM 0.0183716397503386 0.287769072124289 1592 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication nucleoside triphosphate metabolic process nucleotide diphosphatase activity|nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|phosphodiesterase I activity cellular_component YNL333W SNZ2 hom FT COFACTOR METABOLISM 0.0183612229125739 0.287826615255563 1593 Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p pyridoxine metabolic process|thiamine biosynthetic process protein binding cellular_component YNL113W RPC19 het FT NUCLEUS 0.018329361679655 0.288664083794808 1594 RNA polymerase subunit AC19, common to RNA polymerases I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex YCR060W TAH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0182780480472986 0.290016304117091 1595 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|box C/D snoRNP assembly chaperone binding R2TP complex|nucleus|cytoplasm YNL201C PSY2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0182777232244772 0.290096869498826 1596 Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Unknown|response to DNA damage stimulus|protein dephosphorylation|signal transduction involved in meiotic recombination checkpoint protein serine/threonine phosphatase activity condensed nuclear chromosome|nucleus YBL055C YBL055C hom FT RESPONSE TO OXIDATIVE STRESS 0.0182357147979335 0.291135076268448 1597 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases cellular response to oxidative stress|apoptotic DNA fragmentation 3'-5'-exodeoxyribonuclease activity|endonuclease activity cytoplasm YJL135W_d YJL135W hom 0.0181724735741907 0.292811795942382 1598 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Unknown Unknown Unknown YDR138W HPR1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0181689792035391 0.292904631018707 1599 Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|response to DNA damage stimulus|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|Cdc73/Paf1 complex|nucleoplasmic THO complex YBL054W TOD6 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|CHROMOSOME 0.0181688001877838 0.292909387468746 1600 PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|Rpd3L complex|cytoplasm YDL034W_d YDL034W hom 0.0181404728626542 0.293662697731405 1601 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Unknown Unknown Unknown YCR028C FEN2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0181365033869675 0.293768361916539 1602 Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress pantothenate transmembrane transport|endocytosis pantothenate transmembrane transporter activity integral to membrane|plasma membrane YOL115W PAP2 hom FT CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS 0.0181297594922731 0.293947937293054 1603 Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|base-excision repair|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|histone mRNA catabolic process|nuclear polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent antisense transcript catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|snoRNA polyadenylation|tRNA modification 5'-deoxyribose-5-phosphate lyase activity|polynucleotide adenylyltransferase activity nucleolus|nucleus|TRAMP complex YAL056W GPB2 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|MITOCHONDRION|PLASMA MEMBRANE 0.0180974959074764 0.294808064947227 1604 Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication signal transduction|glucose mediated signaling pathway|pseudohyphal growth|ascospore formation|negative regulation of Ras protein signal transduction|invasive growth in response to glucose limitation|positive regulation of transcription from RNA polymerase II promoter cAMP-dependent protein kinase inhibitor activity|signal transducer activity|GTPase activating protein binding mitochondrion|plasma membrane|cytoplasm YDR086C SSS1 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0180971628126858 0.294816953835742 1605 Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation structural molecule activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex|Ssh1 translocon complex YEL019C MMS21 het FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME 0.0180323865566818 0.296548969791656 1606 SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination DNA repair SUMO ligase activity nuclear envelope|Smc5-Smc6 complex|nucleus|cytoplasm YDR506C GMC1 hom FT CELL CYCLE|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS 0.0179080700429399 0.299892023622692 1607 Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein meiosis|synaptonemal complex organization molecular_function cellular_component YDR109C_p YDR109C hom FT CARBOHYDRATE METABOLISM 0.0178553252749358 0.301678940502024 1608 Putative kinase biological_process molecular_function cellular_component YGL261C_p PAU11 hom 0.0178468166756871 0.301620571354112 1609 Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 biological_process molecular_function cellular_component YJL068C YJL068C hom 0.0178297007038947 0.302012350625228 1610 Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D formaldehyde catabolic process S-formylglutathione hydrolase activity cytosol YPL154C PEP4 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0177306136984285 0.304707447461638 1611 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates microautophagy|proteolysis involved in cellular protein catabolic process|cellular response to starvation peptidase activity fungal-type vacuole lumen|mitochondrion|fungal-type vacuole YDR256C CTA1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.017726531375886 0.304818824953933 1612 Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation response to reactive oxygen species|age-dependent response to reactive oxygen species|hydrogen peroxide catabolic process catalase activity peroxisomal matrix|mitochondrial matrix YLR220W CCC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0177216619618833 0.30495171187959 1613 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress manganese ion transport|cellular calcium ion homeostasis|cellular iron ion homeostasis|cellular manganese ion homeostasis|ferrous iron transport manganese ion transmembrane transporter activity|ferrous iron transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|Golgi apparatus YER107C GLE2 hom FF|FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0177199702312197 0.304997888416922 1614 RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 poly(A)+ mRNA export from nucleus|Unknown|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|nuclear pore distribution|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery molecular_function integral to membrane|nuclear pore|cytoplasm YOR171C LCB4 hom FT LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.017714459339255 0.30514834281218 1615 Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes calcium-mediated signaling|sphingolipid metabolic process D-erythro-sphingosine kinase activity Golgi apparatus|endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum YBR134W_d YBR134W hom 0.0177107700824533 0.305249091739105 1616 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR252W_d YLR252W hom 0.0176974166507536 0.305613941424967 1617 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Unknown Unknown Unknown YBR196C-B_p YBR196C-B hom 0.0176862295239536 0.305919824603089 1618 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YIL164C NIT1 hom 0.0176688056442971 0.306468832686639 1619 Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene biological_process nitrilase activity cellular_component YOR311C DGK1 hom FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0176607473492904 0.306689520723546 1620 Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain phosphatidic acid biosynthetic process diacylglycerol kinase activity integral to endoplasmic reticulum membrane|integral to membrane YKL196C YKT6 het FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE 0.0176423579630212 0.307121336782741 1621 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus intra-Golgi vesicle-mediated transport|vacuole fusion, non-autophagic|vesicle fusion palmitoyltransferase activity|SNAP receptor activity SNARE complex|endosome|mitochondrion|Golgi apparatus|fungal-type vacuole|membrane YHR175W-A_p YHR175W-A hom 0.017632155162806 0.307401208355948 1622 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR234C RPL33B hom FT TRANSLATION|RIBOSOME 0.0176167244228901 0.307824807353381 1623 Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL144C YDL144C hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0176110244791848 0.30798137772128 1624 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YDR513W GRX2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0176071337027392 0.308088282755067 1625 Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication cellular response to oxidative stress disulfide oxidoreductase activity|glutathione transferase activity|glutathione peroxidase activity cytosol|mitochondrion YHR185C PFS1 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS|CYTOSKELETON 0.0175795349580158 0.308847304819364 1626 Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation ascospore wall assembly molecular_function cellular_component YJL128C PBS2 hom FF|FT PROTEIN LOCALIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.0175638821252968 0.309278338513049 1627 MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition activation of MAPK activity involved in osmosensory signaling pathway|actin filament organization|MAPK import into nucleus involved in osmosensory signaling pathway|protein phosphorylation|hyperosmotic response|osmosensory signaling pathway|cellular response to heat MAP-kinase scaffold activity|MAP kinase kinase activity|protein kinase activity cellular bud neck|cytoplasm|cellular bud tip YGL010W_p YGL010W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0175420516345615 0.309880150247822 1628 Putative protein of unknown function; YGL010W is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YHR062C RPP1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0175338889622078 0.312500046484042 1629 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YKL063C_p YKL063C hom FT GOLGI APPARATUS 0.0175309932545262 0.310185296768046 1630 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi biological_process molecular_function Golgi apparatus YOR348C PUT4 hom FT PLASMA MEMBRANE 0.0174623304425117 0.312084425241844 1631 Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells neutral amino acid transport|proline transport|gamma-aminobutyric acid transport neutral amino acid transmembrane transporter activity|L-proline transmembrane transporter activity integral to membrane|plasma membrane YGR154C GTO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0174530450056753 0.312341835652017 1632 Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization glutathione metabolic process glutathione transferase activity peroxisome YNR015W SMM1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0174463347253576 0.312527944714498 1633 Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs tRNA processing|tRNA modification tRNA dihydrouridine synthase activity nucleus|cytoplasm YBL077W_d YBL077W het 0.0174280014421125 0.313109013480253 1634 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Unknown Unknown Unknown YKR038C KAE1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|PROTEOLYSIS|NUCLEUS|CHROMOSOME 0.0174241214363602 0.31314454925964 1635 Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription chromosome organization|threonylcarbamoyladenosine metabolic process|telomere maintenance|positive regulation of transcription from RNA polymerase II promoter chromatin DNA binding nuclear chromatin|EKC/KEOPS complex|nucleus|cytoplasm YDR119W_p VBA4 hom 0.0174166583076359 0.313930304690008 1636 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function fungal-type vacuole membrane|integral to membrane YKL192C ACP1 het FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0174012255675862 0.313780938371597 1637 Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p lipoate biosynthetic process ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process mitochondrion YGR189C CRH1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0174011940679158 0.313781814488264 1638 Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress fungal-type cell wall organization|cell wall chitin metabolic process transferase activity, transferring glycosyl groups incipient cellular bud site|fungal-type cell wall YDR305C HNT2 hom FT NUCLEUS|MITOCHONDRION 0.0173970315250868 0.313897603603479 1639 Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins nucleoside catabolic process hydrolase activity|nucleoside-triphosphatase activity mitochondrion|nucleus|cytoplasm YIL046W-A_p YIL046W-A hom 0.0173148727252375 0.316188757790868 1640 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YBL064C PRX1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0173120887727148 0.316627950275157 1641 Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress response to cadmium ion|cellular response to oxidative stress|cell redox homeostasis thioredoxin peroxidase activity mitochondrion YOR007C SGT2 hom FT PROTEIN LOCALIZATION 0.0172680633143315 0.31749902135567 1642 Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress response to heat|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YEL031W SPF1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0172538883206241 0.318257866111064 1643 P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 cellular calcium ion homeostasis|transmembrane transport ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|endoplasmic reticulum membrane|cis-Golgi network YGR072W UPF3 hom FT NUCLEUS 0.0172483755823616 0.318484873460478 1644 Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|translational frameshifting|protein ubiquitination molecular_function nucleolus|nucleus|polysome|cytoplasm YLR289W GUF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION|RIBOSOME 0.017208094337348 0.319182831696655 1645 Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor positive regulation of translation ribosome binding|GTPase activity mitochondrion|mitochondrial ribosome|mitochondrial matrix YFR018C_p YFR018C hom FT PROTEOLYSIS 0.0171989015354041 0.319441462848939 1646 Putative protein of unknown function biological_process molecular_function cellular_component YHR045W_p YHR045W hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.0171916179646178 0.319646476802768 1647 Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum biological_process molecular_function endoplasmic reticulum YKL118W_d YKL118W hom FF 0.017162118021139 0.320694449744433 1648 Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Unknown Unknown Unknown YOR076C SKI7 hom FT TRANSLATION|NUCLEOTIDE METABOLISM 0.0171473318497183 0.320894869543339 1649 Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay|nonfunctional rRNA decay protein binding, bridging Ski complex|cytoplasmic exosome (RNase complex)|cytoplasm YPL189C-A COA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0171460814909952 0.320930162386392 1650 Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p mitochondrial respiratory chain complex IV assembly molecular_function mitochondrial matrix YDR220C_d YDR220C hom 0.0171304480840455 0.321371647629915 1651 Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YGR004W PEX31 hom FT PEROXISOME ORGANIZATION 0.0170972395154828 0.322310767858723 1652 Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function integral to membrane|integral to peroxisomal membrane YLR166C SEC10 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0170810322450928 0.322769749870617 1653 Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion exocytosis|establishment of cell polarity|Golgi to plasma membrane transport|vesicle fusion|vesicle docking involved in exocytosis molecular_function mating projection tip|exocyst YNR009W NRM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0170761176941613 0.32290901170729 1654 Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase negative regulation of transcription involved in G1 phase of mitotic cell cycle repressing transcription factor binding|transcription factor binding transcription factor activity MBF transcription complex|nucleus|cytoplasm YOL162W_p YOL162W hom 0.0170541135600398 0.323533014964347 1655 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YOR284W HUA2 hom 0.0170499787051768 0.323650360670844 1656 Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YIR024C YIR024C hom FT MITOCHONDRION 0.0170271620427586 0.324298387866996 1657 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect biological_process molecular_function mitochondrion YOR127W RGA1 hom FT SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.01700886536119 0.324818650598947 1658 GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress budding cell isotropic bud growth|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction|invasive growth in response to glucose limitation|budding cell apical bud growth Rho GTPase activator activity cellular bud neck split septin rings YER059W PCL6 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0169929689283975 0.325415545586642 1659 Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding regulation of glycogen catabolic process|regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YGR056W RSC1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|RSC COMPLEX 0.0169825959097548 0.325566566448581 1660 Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook double-strand break repair|ATP-dependent chromatin remodeling|regulation of sporulation resulting in formation of a cellular spore|double-strand break repair via nonhomologous end joining|nucleosome disassembly|transcription elongation from RNA polymerase II promoter DNA-dependent ATPase activity RSC complex YLR037C PAU23 hom FT CELL WALL ORG/BIOGENESIS 0.0169743971861108 0.326161472308992 1661 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function fungal-type cell wall YPL028W ERG10 het FT LIPID METABOLISM 0.0169261536822127 0.327177313027334 1662 Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis ergosterol biosynthetic process acetyl-CoA C-acetyltransferase activity cytosol YDR085C AFR1 hom FT SIGNALING 0.0169074097886142 0.3277133677747 1663 Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication regulation of G-protein coupled receptor protein signaling pathway|pheromone-dependent signal transduction involved in conjugation with cellular fusion|cell morphogenesis involved in conjugation with cellular fusion|cell morphogenesis involved in conjugation receptor signaling protein activity mating projection base YKL202W_d YKL202W hom 0.0169070807461513 0.327722783112948 1664 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR338C_p YDR338C hom 0.0168677984998955 0.328848080159973 1665 Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily transmembrane transport antiporter activity integral to membrane YOR324C FRT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0168668666723006 0.328874804079305 1666 Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication response to stress molecular_function endoplasmic reticulum YNL148C ALF1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS|CYTOSKELETON 0.016852556429471 0.329791130857302 1667 Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance protein folding|post-chaperonin tubulin folding pathway microtubule binding|alpha-tubulin binding nucleus|cytoplasm YCL026C-B HBN1 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0168112265461607 0.330473059655973 1668 Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress biological_process NAD(P)H nitroreductase activity nucleus|cytoplasm YPR184W GDB1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0167930513944401 0.330996225482057 1669 Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress glycogen catabolic process 4-alpha-glucanotransferase activity|amylo-alpha-1,6-glucosidase activity mitochondrion|cytoplasm YJL155C FBP26 hom FT CARBOHYDRATE METABOLISM 0.0167835611933955 0.331413973053877 1670 Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress glucose metabolic process 6-phosphofructo-2-kinase activity|fructose-2,6-bisphosphate 2-phosphatase activity cytosol YBR172C SMY2 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0167745642530357 0.331528920877013 1671 Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum membrane|cytoplasmic mRNA processing body|cytoplasm|extrinsic to membrane YNL146C-A_p YNL146C-A hom 0.0167458651632854 0.332356964075956 1672 Putative protein of unknown function biological_process molecular_function cellular_component YJL077C ICS3 hom 0.016728905153466 0.332919088407122 1673 Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 biological_process molecular_function cellular_component YPR004C AIM45 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.016693037013826 0.33388468147145 1674 Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response biological_process molecular_function mitochondrion YJR096W YJR096W hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0166903131187656 0.333963575309381 1675 Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS cellular response to oxidative stress|D-xylose catabolic process|arabinose catabolic process alditol:NADP+ 1-oxidoreductase activity|aldo-keto reductase (NADP) activity nucleus|cytoplasm YDR019C GCV1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0166820694863841 0.334202413885622 1676 T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm glycine catabolic process|glycine metabolic process|one-carbon metabolic process glycine dehydrogenase (decarboxylating) activity mitochondrion|glycine cleavage complex YOL037C_d YOL037C hom 0.0166566935803576 0.335010444033994 1677 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Unknown Unknown Unknown YLR066W SPC3 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0166500645127274 0.335130720768376 1678 Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex YOR022C_p YOR022C hom FT LIPID METABOLISM|MITOCHONDRION 0.0166376658379744 0.335490791093265 1679 Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process phospholipase activity mitochondrion YDR226W ADK1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0165957780072279 0.336709097834895 1680 Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress ADP biosynthetic process|DNA replication initiation|nucleotide metabolic process adenylate kinase activity mitochondrion|mitochondrial intermembrane space|cytoplasm YBR216C YBP1 hom FT RESPONSE TO OXIDATIVE STRESS 0.016522358008431 0.3389234598475 1681 Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication cellular response to oxidative stress molecular_function cytoplasm YJL060W BNA3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0165091668497652 0.33923718946698 1682 Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate kynurenic acid biosynthetic process arylformamidase activity|kynurenine-oxoglutarate transaminase activity mitochondrion|cytoplasm YPR120C CLB5 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.016492700229457 0.339719206816724 1683 B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase spindle assembly|G1/S transition of mitotic cell cycle|regulation of S phase of mitotic cell cycle|G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation|premeiotic DNA replication|positive regulation of DNA replication cyclin-dependent protein kinase regulator activity nucleus YLR319C BUD6 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0164526634903166 0.340893007961981 1684 Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate bipolar cellular bud site selection|positive regulation of formin-nucleated actin cable assembly|cellular bud site selection|Rho protein signal transduction|extrachromosomal circular DNA localization involved in cell aging|establishment of cell polarity|response to osmotic stress|cytokinesis cytoskeletal regulatory protein binding polarisome|actin cap|spindle pole body|mating projection tip YMR226C YMR226C hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0164501446221455 0.34096694309796 1685 NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments metabolic process oxidoreductase activity|oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor|carbonyl reductase (NADPH) activity integral to membrane|ribosome|nucleus|cytoplasm YMR244C-A COA6 hom FT NUCLEUS|MITOCHONDRION 0.0164440454201831 0.342953681892798 1686 Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YDR295C HDA2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.016442486650299 0.341191786926239 1687 Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance negative regulation of transcription from RNA polymerase II promoter|gene silencing by RNA|histone deacetylation|gene silencing involved in chronological cell aging histone deacetylase activity|chromatin binding|DNA binding nucleus|HDA1 complex YGR149W_p YGR149W hom 0.0164348682126338 0.341415564128558 1688 Putative protein of unknown function; predicted to be an integal membrane protein biological_process molecular_function integral to membrane YOR118W RTC5 hom 0.0164279320680553 0.341619381906186 1689 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity biological_process molecular_function cytoplasm YDR522C SPS2 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0164060195552611 0.342263789677268 1690 Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component ascospore wall assembly|ascospore formation molecular_function fungal-type cell wall|plasma membrane YGL166W CUP2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0163861902022023 0.343063771655901 1691 Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter|response to copper ion RNA polymerase II core promoter proximal region sequence-specific DNA binding|copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity|copper ion binding nucleus YBR101C FES1 hom FT TRANSLATION|RIBOSOME 0.0163850041415027 0.342882544852809 1692 Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress cytoplasmic translation adenyl-nucleotide exchange factor activity cytosol|cytosolic ribosome YDR399W HPT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0163283637624905 0.344553758962024 1693 Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome GMP salvage|IMP salvage hypoxanthine phosphoribosyltransferase activity nucleus|cytoplasm YER005W YND1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.016306725280153 0.345193586634197 1694 Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity protein glycosylation nucleoside-triphosphatase activity|nucleoside-diphosphatase activity COPI-coated vesicle|Golgi apparatus|membrane|Golgi membrane YDL018C ERP3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0162694545547543 0.346297417832053 1695 Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport vesicle-mediated transport molecular_function integral to membrane YIL076W SEC28 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0162215373906691 0.347719857737788 1696 Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport|vesicle coating molecular_function endosome|COPI vesicle coat YOR068C VAM10 hom FT MEMBRANE ORGANIZATION 0.0161926108740179 0.348580348034729 1697 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole fusion, non-autophagic molecular_function fungal-type vacuole membrane YER022W SRB4 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0161913404634696 0.348618170494597 1698 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination transcription initiation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter activating transcription factor binding|structural molecule activity|core RNA polymerase II recruiting transcription factor activity core mediator complex YMR143W RPS16A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0161852357686544 0.348799954845363 1699 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YGR228W_d YGR228W hom 0.0161795175540388 0.348970285252257 1700 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Unknown Unknown Unknown YKL006W RPL14A hom FT TRANSLATION|RIBOSOME 0.0161726443472646 0.3492470190358 1701 Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YHR153C SPO16 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0161612789772955 0.351752933882853 1702 Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation synaptonemal complex assembly|ascospore formation|regulation of reciprocal meiotic recombination molecular_function condensed nuclear chromosome YDR476C_p YDR476C hom FT ENDOPLASMIC RETICULUM 0.0161543092106937 0.349937622094669 1703 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene biological_process molecular_function endoplasmic reticulum YIR019C FLO11 hom FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0161441212346563 0.350025822330701 1704 GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain cell-cell adhesion|biofilm formation|pseudohyphal growth|filamentous growth|flocculation|invasive growth in response to glucose limitation molecular_function extracellular region|plasma membrane|cellular bud neck YLL037W_d YLL037W het 0.0161427025761959 0.350068169639344 1705 Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Unknown Unknown Unknown YNR029C_p YNR029C hom 0.0161401025648834 0.350145789058333 1706 Putative protein of unknown function, deletion confers reduced fitness in saline biological_process molecular_function cytoplasm YOR197W MCA1 hom FT PROTEOLYSIS|NUCLEUS 0.016112728550328 0.350963661198704 1707 Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization misfolded or incompletely synthesized protein catabolic process|apoptotic process cysteine-type peptidase activity nucleus YDR418W RPL12B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0160992090746687 0.351368037945983 1708 Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YOR015W_d YOR015W hom 0.0160824275794383 0.351870394196175 1709 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER093C-A AIM11 hom 0.0160748421988167 0.352097612667986 1710 Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication biological_process molecular_function cellular_component YAL062W GDH3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS|MITOCHONDRION 0.0160513358291141 0.352802330535613 1711 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication glutamate biosynthetic process|ammonia assimilation cycle glutamate dehydrogenase (NADP+) activity mitochondrion|nucleus YMR165C PAH1 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0160177319378536 0.353811318529147 1712 Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 plasmid maintenance|vacuole fusion, non-autophagic|lipid particle organization|aerobic respiration|phospholipid biosynthetic process phosphatidate phosphatase activity cytosol|nuclear membrane|vacuole|cytoplasm|extrinsic to membrane YDL020C RPN4 hom FF|FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0160137824110059 0.354721314757162 1713 Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress positive regulation of proteasomal ubiquitin-dependent protein catabolic process|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|regulation of DNA repair|negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YPL101W ELP4 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0160050261585402 0.354193295778444 1714 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification ATPase activity|tRNA binding Elongator holoenzyme complex|cytoplasm YAR007C RFA1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.015968586251076 0.355793304777786 1715 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|DNA replication|double-strand break repair via homologous recombination|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex|cytoplasm YJL074C SMC3 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0159680730059641 0.355305707603034 1716 Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member synaptonemal complex assembly|ascospore formation|reciprocal meiotic recombination|mitotic sister chromatid cohesion|meiotic sister chromatid cohesion ATPase activity nucleus|nuclear mitotic cohesin complex YJR104C SOD1 hom FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0159675552192318 0.355393116065617 1717 Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid cellular copper ion homeostasis|fungal-type cell wall organization|superoxide metabolic process|cellular zinc ion homeostasis|positive regulation of sequence-specific DNA binding transcription factor activity|age-dependent response to reactive oxygen species involved in chronological cell aging superoxide dismutase activity cytosol|mitochondrion|nucleus|mitochondrial intermembrane space YBR187W_p GDT1 hom 0.0159606630626459 0.355888241084603 1718 Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis biological_process molecular_function integral to membrane|fungal-type vacuole YDR379W RGA2 hom FT SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0159519055651868 0.355793093104251 1719 GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p budding cell isotropic bud growth|pheromone-dependent signal transduction involved in conjugation with cellular fusion|actin filament organization|pseudohyphal growth|establishment of cell polarity|small GTPase mediated signal transduction|invasive growth in response to glucose limitation|budding cell apical bud growth Rho GTPase activator activity cellular bud neck split septin rings YNR014W_p YNR014W hom 0.0159317738239672 0.356400575699336 1720 Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication biological_process molecular_function cytoplasm YMR090W_p YMR090W hom 0.0159241657445402 0.356630322292347 1721 Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability biological_process molecular_function cytoplasm YNL188W KAR1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0159168211459 0.357355019804088 1722 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p karyogamy involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope protein binding half bridge of spindle pole body YNL010W_p YNL010W hom FT NUCLEUS 0.0159094412530008 0.357075233151166 1723 Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation biological_process molecular_function nucleus|cytoplasm YOL118C_d YOL118C hom 0.0158781502928878 0.358237165668363 1724 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR183W DPM1 het FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0158690765156241 0.358368411682813 1725 Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation GPI anchor biosynthetic process|protein O-linked glycosylation|protein N-linked glycosylation dolichyl-phosphate beta-D-mannosyltransferase activity mitochondrial outer membrane|mitochondrion|endoplasmic reticulum|nuclear outer membrane-endoplasmic reticulum membrane network YBR040W FIG1 hom FT SITE OF POLARIZED GROWTH 0.0158638045042592 0.358456398202125 1726 Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating cell morphogenesis involved in conjugation with cellular fusion|cytogamy molecular_function fungal-type cell wall|integral to membrane|mating projection tip YIR032C DAL3 hom FT PLASMA MEMBRANE 0.0158573894372437 0.358650814703093 1727 Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression allantoin catabolic process ureidoglycolate hydrolase activity membrane YPL224C MMT2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0158497358134793 0.358882853877041 1728 Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p cellular iron ion homeostasis molecular_function mitochondrion|integral to membrane YLR187W SKG3 hom FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0158430195032815 0.359086553687269 1729 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication biological_process molecular_function cellular bud|cellular bud neck|cytoplasm YEL028W_d YEL028W hom 0.0157957741204211 0.360593202175268 1730 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL082C MOT1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0157900561202458 0.360695430662289 1731 Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA regulation of RNA polymerase II transcriptional preinitiation complex assembly|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of transcription from RNA polymerase II promoter|rRNA processing TBP-class protein binding|ATPase activity nuclear chromosome|mitochondrion YPL073C_d YPL073C hom 0.0157659981214533 0.361427735151928 1732 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Unknown Unknown Unknown YKR005C_p YKR005C hom 0.0157427026695119 0.362137715632045 1733 Putative protein of unknown function biological_process molecular_function cellular_component YLL005C SPO75 hom FT CELL WALL ORG/BIOGENESIS 0.0157371785495829 0.362306203443067 1734 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function integral to membrane YMR292W GOT1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0157356920140006 0.362638228399739 1735 Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition ER to Golgi vesicle-mediated transport|Golgi to endosome transport molecular_function integral to membrane|endoplasmic reticulum|Golgi apparatus|ER to Golgi transport vesicle|Golgi membrane YGL233W SEC15 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0157349545174972 0.362374051144394 1736 Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis Rab GTPase binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YPL241C CIN2 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON 0.0157076348227701 0.363208131587364 1737 GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 protein folding|tubulin complex assembly|tubulin complex biogenesis GTPase activator activity cellular_component YBL039C URA7 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0156881266258892 0.36416266508979 1738 Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YJL030W MAD2 hom FT CELL CYCLE|CELL DIVISION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|NUCLEAR PORE|KINETOCHORE 0.0156475694691079 0.365046167653711 1739 Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I chromosome decondensation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex|condensed nuclear chromosome kinetochore|nuclear pore YML076C WAR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0156212321039076 0.365853935055145 1740 Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively response to acid sequence-specific DNA binding transcription factor activity mitochondrion|nucleus YLR034C SMF3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0156122987516058 0.366128174143025 1741 Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress cellular iron ion homeostasis|iron ion transport metal ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YGR130C YGR130C hom 0.0156031032819387 0.366625248868684 1742 Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) biological_process molecular_function eisosome|membrane raft|cytoplasm YOR101W RAS1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0155810702196141 0.367159358777073 1743 GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress adenylate cyclase-activating G-protein coupled receptor signaling pathway|replicative cell aging|Ras protein signal transduction GTPase activity plasma membrane YGR148C RPL24B hom FT TRANSLATION|RIBOSOME 0.0155803777645724 0.36710914246808 1744 Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YPR154W PIN3 hom FT CYTOSKELETON ORGANIZATION|NUCLEUS 0.01555904750636 0.367765557324695 1745 Protein that induces appearance of [PIN+] prion when overproduced actin cytoskeleton organization molecular_function nucleus|cytoplasm YPR107C YTH1 het FF|FT RNA PROCESSING|NUCLEUS 0.0155333672954715 0.368914359527699 1746 Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YBR045C GIP1 hom FT CELL WALL ORG/BIOGENESIS 0.015474813062972 0.370364911516929 1747 Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p ascospore wall assembly|regulation of phosphoprotein phosphatase activity protein phosphatase type 1 regulator activity|protein phosphatase 1 binding protein phosphatase type 1 complex|prospore membrane YER012W PRE1 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0154588926754102 0.370857470328822 1748 Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activator activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YHR057C CPR2 hom 0.015457788546611 0.370891645892088 1749 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity cellular_component YGR061C ADE6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0154474225822676 0.371212593839823 1750 Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway 'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process phosphoribosylformylglycinamidine synthase activity cytoplasm YDR320C SWA2 hom FF|FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0154072117916892 0.372459217927156 1751 Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles positive regulation of ATPase activity|endoplasmic reticulum inheritance|clathrin coat disassembly ubiquitin binding|clathrin binding endoplasmic reticulum membrane YDR437W GPI19 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0153717210489649 0.373561661730505 1752 Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|nuclear envelope|integral to endoplasmic reticulum membrane|endoplasmic reticulum YGR167W CLC1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.015353523073632 0.374341697317998 1753 Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W endocytosis|vesicle-mediated transport structural molecule activity clathrin vesicle coat YGR248W SOL4 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0153498297040353 0.374313976463762 1754 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication pentose-phosphate shunt, oxidative branch 6-phosphogluconolactonase activity cytosol|nucleus|cytoplasm YFL056C AAD6 hom FT OXIDATION-REDUCTION PROCESS 0.0153351228307733 0.374700618791557 1755 Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YNL133C FYV6 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0153211900215657 0.37513477896504 1756 Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining double-strand break repair via nonhomologous end joining molecular_function nucleus YGR033C TIM21 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0153085628420167 0.37552852269832 1757 Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity protein import into mitochondrial matrix molecular_function mitochondrion|mitochondrial inner membrane presequence translocase complex YJR142W_p YJR142W hom 0.0152998490863334 0.37580038584162 1758 Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member biological_process molecular_function cellular_component YHR206W SKN7 hom FF|FT TRANSCRIPTION FROM RNA POL II|SIGNALING|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0152948082536097 0.376100224408818 1759 Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication response to singlet oxygen|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|transcription, DNA-dependent|response to osmotic stress|regulation of cell size sequence-specific DNA binding|two-component response regulator activity|sequence-specific DNA binding transcription factor activity nucleus YER017C AFG3 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0152749772369201 0.376577038270321 1760 Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging proteolysis|signal peptide processing|protein complex assembly|protein import into mitochondrial intermembrane space metallopeptidase activity|protein binding|ATPase activity mitochondrion|mitochondrial inner boundary membrane|mitochondrial inner membrane|m-AAA complex YMR140W SIP5 hom FT RESPONSE TO NUTRIENT LEVELS 0.0152616895458304 0.376992367238728 1761 Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress cellular response to glucose starvation molecular_function cytoplasm YNL112W DBP2 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0152366460933033 0.378132035059127 1762 ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|rRNA processing RNA helicase activity mitochondrion|nucleus|cytoplasm YOR264W DSE3 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH 0.0152309172947642 0.377955288860898 1763 Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud neck YNR053C NOG2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0152192184818972 0.378535327810212 1764 Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation ribosomal large subunit export from nucleus GTPase activity nucleoplasm|nucleolus|nucleus|preribosome, large subunit precursor YBR041W FAT1 hom FT KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0151778384317783 0.379619781557848 1765 Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids very long-chain fatty acid metabolic process|long-chain fatty acid transport very long-chain fatty acid-CoA ligase activity|long-chain fatty acid transporter activity intrinsic to internal side of plasma membrane|integral to membrane|endoplasmic reticulum|lipid particle|peroxisome YPL245W_p YPL245W hom FT NUCLEUS 0.0151640382010569 0.380053277969459 1766 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus|cytoplasm YOR043W WHI2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0151079804374275 0.381817303628036 1767 Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression response to salt stress|mitochondrion degradation|actin filament organization|endocytosis|response to heat|regulation of growth|response to stress phosphatase activator activity cellular_component YLR181C VTA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|VACUOLAR TRANSPORT 0.0150886599729593 0.382426441495985 1768 Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain late endosome to vacuole transport via multivesicular body sorting pathway|positive regulation of protein oligomerization|late endosome to vacuole transport ATPase activator activity endosome|endosome membrane|multivesicular body YLL004W ORC3 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.0150542817197125 0.383511794585477 1769 Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YNL303W_d YNL303W hom 0.0150528497322382 0.384125467453828 1770 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR045C_p YKR045C hom 0.0150292457455162 0.385013832828899 1771 Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm YDR216W ADR1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|PEROXISOME ORGANIZATION|NUCLEUS 0.0150286665267354 0.384321714214787 1772 Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization negative regulation of chromatin silencing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|chromatin organization|regulation of transcription by chromatin organization|positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by oleic acid|cellular response to oleic acid|peroxisome organization|positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|TFIIB-class transcription factor binding|sequence-specific DNA binding|TFIID-class transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription nucleus YMR121C RPL15B hom FT TRANSLATION|RIBOSOME 0.014975964709251 0.385991364846587 1773 Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDR035W ARO3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS|MITOCHONDRION 0.0149333925536087 0.387768690031894 1774 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity mitochondrion|nucleus|cytoplasm YDR432W NPL3 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|NUCLEUS 0.0149279890134113 0.3881533699076 1775 RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress translational termination|mRNA export from nucleus|negative regulation of translation|mRNA splicing, via spliceosome|Unknown|positive regulation of transcription elongation from RNA polymerase II promoter mRNA binding|RNA polymerase II core binding|eukaryotic initiation factor 4G binding|poly(A) RNA binding nucleus|cytoplasm YPR032W SRO7 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|PLASMA MEMBRANE 0.0149266369699035 0.387558125791847 1776 Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication establishment of cell polarity|exocytosis|Golgi to plasma membrane transport|small GTPase mediated signal transduction SNARE binding|Rab GTPase binding cytosol|plasma membrane YLR425W TUS1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH 0.0149110331264469 0.388054544949375 1777 Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate fungal-type cell wall organization|signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|cellular bud neck YBR227C MCX1 hom FT MITOCHONDRION 0.0147512210119539 0.39316105533544 1778 Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein refolding molecular_function mitochondrion|mitochondrial matrix YIR021W-A_p YIR021W-A hom 0.0147420788535971 0.393454401994441 1779 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YGL153W PEX14 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0147371372061954 0.39361302098999 1780 Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p protein import into peroxisome matrix, docking protein binding, bridging peroxisomal membrane YAL003W EFB1 het FT TRANSLATION|RIBOSOME 0.0147141784875831 0.394421038902504 1781 Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site translational elongation translation elongation factor activity|guanyl-nucleotide exchange factor activity ribosome|eukaryotic translation elongation factor 1 complex YMR158C-A_p YMR158C-A hom 0.014696467808425 0.394919913260231 1782 Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene biological_process molecular_function cellular_component YDR373W FRQ1 het FT SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0146952980451173 0.394957541822923 1783 N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily regulation of signal transduction enzyme activator activity|calcium ion binding Golgi membrane YDR080W VPS41 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING 0.0146936605635657 0.395010219432316 1784 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole organization|vesicle-mediated transport|protein transport|vacuole fusion, non-autophagic|vacuolar protein processing Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding endosome|fungal-type vacuole membrane|HOPS complex YMR244W_p YMR244W hom 0.0146916031930433 0.39507641084421 1785 Putative protein of unknown function biological_process molecular_function cellular_component YDR291W HRQ1 hom FT NUCLEUS 0.014649967969027 0.397972266601512 1786 3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) biological_process molecular_function nucleus YOR327C SNC2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.014614767307261 0.397553234843234 1787 Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication endocytosis|exocytosis|Golgi to plasma membrane transport|vesicle fusion SNAP receptor activity SNARE complex|trans-Golgi network|endosome|transport vesicle membrane YPL215W CBP3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0146145641913909 0.397559794710336 1788 Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex positive regulation of mitochondrial translation|mitochondrial respiratory chain complex III assembly ribosome binding integral to mitochondrial membrane|mitochondrion|mitochondrial ribosome YOR207C RET1 het FF|FT NUCLEUS 0.0145599282131022 0.399326695934237 1789 Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YKR016W FCJ1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0145315579188066 0.400246037528992 1790 Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane cristae formation|protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial crista|mitochondrial crista junction|integral to mitochondrial inner membrane YJR057W CDC8 het FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0145105492020506 0.400927645407123 1791 Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p dTDP biosynthetic process|dTTP biosynthetic process|dUDP biosynthetic process uridylate kinase activity|thymidylate kinase activity nucleus|cytoplasm YIL092W_p YIL092W hom FT NUCLEUS 0.0144997840127543 0.401277181618544 1792 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YOR113W AZF1 hom FT TRANSCRIPTION FROM RNA POL II|CELL WALL ORG/BIOGENESIS|NUCLEUS 0.0144359550150835 0.403353406872887 1793 Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity regulation of fungal-type cell wall organization|regulation of transcription from RNA polymerase II promoter|cellular response to carbohydrate stimulus RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YLR347C KAP95 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0143790323792525 0.405490904761498 1794 Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p protein targeting to membrane|regulation of protein desumoylation|protein import into nucleus|phosphatidylcholine biosynthetic process|nuclear pore complex assembly protein transporter activity|Ran guanyl-nucleotide exchange factor activity integral to membrane|nuclear exosome (RNase complex)|nuclear pore|cytoplasm YFR034C PHO4 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.014306073922663 0.407878042557898 1795 Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability cellular response to phosphate starvation|positive regulation of phosphate metabolic process|phosphate-containing compound metabolic process|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YLR174W IDP2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.0143057038624635 0.407820129961778 1796 Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication glutamate biosynthetic process|isocitrate metabolic process isocitrate dehydrogenase (NADP+) activity cytosol YNL255C GIS2 hom FT TRANSLATION|RIBOSOME 0.014274236190661 0.408642496500381 1797 Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 positive regulation of translation mRNA binding|translation regulator activity|single-stranded DNA binding polysomal ribosome|cytoplasm YGR236C SPG1 hom FT MITOCHONDRION 0.0142741967258045 0.408643792233673 1798 Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YNL176C_p TDA7 hom 0.0142568025492677 0.409215126024557 1799 Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function fungal-type vacuole YHR047C AAP1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0142501108741036 0.409435049054711 1800 Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication glycogen metabolic process|proteolysis aminopeptidase activity nucleus|cytoplasm YMR109W MYO5 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0142237328646573 0.410302651007378 1801 One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization bipolar cellular bud site selection|endocytosis|exocytosis|fungal-type cell wall organization|response to salt stress|receptor-mediated endocytosis|actin cortical patch localization|response to osmotic stress microfilament motor activity mating projection tip|actin cortical patch YIL144W TID3 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0141845019081114 0.411595018473347 1802 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering microtubule nucleation|chromosome segregation|protein localization to kinetochore structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YIL085C KTR7 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0141561281050807 0.412531226419563 1803 Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication fungal-type cell wall organization|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YCL049C_p YCL049C hom 0.0141521281026792 0.412663309907939 1804 Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane YCL012C YCL012C hom 0.0141481061257573 0.413424430557747 1805 Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene biological_process molecular_function cellular_component YKL167C MRP49 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0141001869079989 0.414380725975176 1806 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YPL156C PRM4 hom 0.0140667498277585 0.415488542651509 1807 Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function integral to membrane YLR050C_p YLR050C hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0140562378114983 0.415837181239696 1808 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YMR144W_p YMR144W hom FT NUCLEUS 0.014055526500778 0.41586077860712 1809 Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene biological_process molecular_function nucleus YDR423C CAD1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0140285902106455 0.416754957660703 1810 AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication cellular response to cadmium ion|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YOR191W ULS1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0140269633256841 0.416809000126907 1811 Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress chromatin assembly or disassembly|mating type switching SUMO binding|DNA-dependent ATPase activity mitochondrion|nucleolus|nucleus YAL018C_p YAL018C hom 0.0140199111694203 0.417112836343993 1812 Putative protein of unknown function biological_process molecular_function integral to membrane YGL186C TPN1 hom FF|FT PLASMA MEMBRANE 0.014013393769724 0.417468520000081 1813 Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p vitamin transport vitamin transporter activity integral to membrane|plasma membrane YBR068C BAP2 hom FT PLASMA MEMBRANE 0.0139856166189069 0.418183856538409 1814 High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity integral to membrane|plasma membrane YOL026C MIM1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0139789377883586 0.418406190461257 1815 Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane molecular_function mitochondrial outer membrane|integral to mitochondrial outer membrane YDR536W STL1 hom FT PLASMA MEMBRANE 0.013907205432091 0.420798495961357 1816 Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock transport|glycerol transport|transmembrane transport solute:hydrogen symporter activity|transporter activity integral to membrane|plasma membrane|membrane YJL109C UTP10 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0138913768024565 0.421604789382218 1817 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding mitochondrion|90S preribosome|small-subunit processome|preribosome, small subunit precursor|integral to membrane|t-UTP complex|rDNA heterochromatin|nucleolus YKL018W SWD2 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0138852401019409 0.421532651426561 1818 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination mRNA 3'-end processing|termination of RNA polymerase II transcription, exosome-dependent|histone H3-K4 methylation|termination of RNA polymerase II transcription, poly(A)-coupled|telomere maintenance|snoRNA 3'-end processing|histone H3-K79 methylation histone methyltransferase activity (H3-K4 specific) mRNA cleavage and polyadenylation specificity factor complex|Set1C/COMPASS complex YHL003C LAG1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0138704699950688 0.422303914829555 1819 Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress replicative cell aging|ceramide biosynthetic process sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YJL137C GLG2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0138628204029509 0.422698549599096 1820 Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication glycogen biosynthetic process glycogenin glucosyltransferase activity cytoplasm YLR422W_p YLR422W hom 0.0138325513682702 0.423296744826017 1821 Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein biological_process molecular_function cytoplasm YDR353W TRR1 het FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0137947206645955 0.424566030130603 1822 Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication cellular response to oxidative stress|cell redox homeostasis ferrous iron binding|thioredoxin-disulfide reductase activity cytosol YNL065W AQR1 hom FT PLASMA MEMBRANE 0.0137703698588318 0.425384216529806 1823 Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress monocarboxylic acid transport|drug transmembrane transport|amino acid export monocarboxylic acid transmembrane transporter activity|drug transmembrane transporter activity integral to membrane|plasma membrane YAL065C_p YAL065C hom 0.0137676639886295 0.425475190469085 1824 Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component YDR527W RBA50 het FF|FT TRANSCRIPTION FROM RNA POL II 0.0137361242196405 0.426536424788536 1825 Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 transcription from RNA polymerase II promoter molecular_function cytoplasm YPL136W_d YPL136W hom 0.0137268064022834 0.426850240997305 1826 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Unknown Unknown Unknown YGL185C_p YGL185C hom FT OXIDATION-REDUCTION PROCESS 0.0137227810728954 0.427331020330407 1827 Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm metabolic process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor cytoplasm YLR291C GCD7 het FT TRANSLATION|MITOCHONDRION 0.0136479309264769 0.429512084204322 1828 Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|translation initiation factor activity|guanyl-nucleotide exchange factor activity mitochondrion|eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YHR155W YSP1 hom FF|FT MITOCHONDRION 0.0136120925865308 0.430724713717364 1829 Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication biological_process molecular_function mitochondrion YMR296C LCB1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0135671940832273 0.432246702076142 1830 Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine sphingolipid biosynthetic process serine C-palmitoyltransferase activity SPOTS complex|serine C-palmitoyltransferase complex YDR367W KEI1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0135415885375528 0.433184736555236 1831 Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene inositolphosphoceramide metabolic process inositol phosphoceramide synthase regulator activity integral to membrane|Golgi apparatus|integral to Golgi membrane|inositol phosphoceramide synthase complex|cytoplasm YMR102C_p YMR102C hom 0.0135091461884368 0.434219041071157 1832 Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene biological_process molecular_function cellular_component YER076C_p YER076C hom FT MITOCHONDRION 0.0134106452943739 0.437577748719577 1833 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization biological_process molecular_function mitochondrion YDL207W GLE1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|NUCLEAR PORE 0.0133722146759387 0.438892206890891 1834 Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export poly(A)+ mRNA export from nucleus|mRNA export from nucleus|regulation of translational initiation|regulation of translational termination translation initiation factor binding|inositol hexakisphosphate binding|phospholipid binding|enzyme activator activity mitochondrion|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore|cytoplasm YIL013C PDR11 hom FT NUCLEOTIDE METABOLISM 0.0133550385033267 0.439821987799497 1835 ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth sterol transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YBR138C_p YBR138C hom 0.0133484606618921 0.43970580779876 1836 Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm YDL212W SHR3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0132985746362477 0.441417272320311 1837 Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface protein folding|ER to Golgi vesicle-mediated transport unfolded protein binding integral to endoplasmic reticulum membrane|integral to membrane|mating projection tip YLR287C-A RPS30A hom FT TRANSLATION|RIBOSOME 0.0132920841938703 0.441640223161911 1838 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasmic stress granule YBR072W HSP26 hom FT NUCLEUS 0.0132580114101921 0.4428117017897 1839 Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity protein folding|response to stress unfolded protein binding|mRNA binding nucleus|cytoplasm YPR180W AOS1 het FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0132332719762224 0.44502698027494 1840 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity nucleus|SUMO activating enzyme complex YOL140W ARG8 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.013197766765455 0.44549948066668 1841 Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine arginine biosynthetic process|ornithine biosynthetic process N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity mitochondrion|mitochondrial matrix YBR156C SLI15 hom FT CELL DIVISION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.013194493294888 0.44554428750741 1842 Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly chromosome segregation|protein phosphorylation|regulation of cytokinesis protein kinase activator activity spindle microtubule|spindle midzone|chromosome passenger complex|kinetochore microtubule YDL108W KIN28 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.013194207297986 0.445078104641996 1843 Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase I promoter|protein phosphorylation|transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activating kinase activity|protein kinase activity TFIIK complex|holo TFIIH complex YOL087C DUF1 hom 0.013164179119066 0.446046974645318 1844 Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid biological_process ubiquitin binding cytoplasm YML103C NUP188 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0131494353375388 0.446556550537728 1845 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 nuclear pore organization|protein import into nucleus|mRNA export from nucleus in response to heat stress structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YOR020C HSP10 het FT MITOCHONDRION 0.0130672329556726 0.449403699889737 1846 Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES protein folding|protein refolding unfolded protein binding|chaperone binding mitochondrion|mitochondrial matrix YJL091C GWT1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0130629377226019 0.450230024834281 1847 Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors GPI anchor biosynthetic process glucosaminyl-phosphotidylinositol O-acyltransferase activity integral to membrane|nuclear outer membrane-endoplasmic reticulum membrane network YAL015C NTG1 hom FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0130620620828302 0.449583140830977 1848 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication DNA repair|cellular response to oxidative stress|base-excision repair|base-excision repair, AP site formation|positive regulation of mitochondrial DNA replication oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity|oxidized purine nucleobase lesion DNA N-glycosylase activity|DNA-(apurinic or apyrimidinic site) lyase activity mitochondrion|nucleus YIL002W-A_p YIL002W-A hom 0.0130319942773099 0.450627365677008 1849 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YGL191W COX13 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0129529735901215 0.453445618532271 1850 Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes mitochondrial respiratory chain supercomplex assembly|aerobic respiration enzyme regulator activity|cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YLR006C SSK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.0129291880825408 0.454208074851831 1851 Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate activation of MAPKKK activity involved in osmosensory signaling pathway|positive regulation of protein autophosphorylation|osmosensory signaling pathway via two-component system protein kinase activator activity|two-component response regulator activity|protein homodimerization activity|mitogen-activated protein kinase kinase kinase binding cytoplasm YMR032W HOF1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0128975872012128 0.455311937196954 1852 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p cytokinesis after mitosis|barrier septum assembly involved in cell cycle cytokinesis|response to DNA damage stimulus|cytokinesis cytoskeletal protein binding cellular bud neck contractile ring|cellular bud neck septin ring YMR043W MCM1 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|NUCLEUS|CHROMOSOME 0.0128868492947129 0.455687370424886 1853 Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes regulation of mating type switching|positive regulation of transcription involved in G2/M-phase of mitotic cell cycle|DNA replication initiation|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of mating-type specific transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA replication origin binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II repressing transcription factor binding nuclear chromatin YDR263C DIN7 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0128620278048302 0.456959307134904 1854 Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination DNA repair nuclease activity mitochondrion YLR374C_d YLR374C hom 0.0128480062693151 0.45758467280517 1855 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Unknown Unknown Unknown YOR337W TEA1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0128149096492352 0.458408420364777 1856 Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein transcription, DNA-dependent sequence-specific DNA binding|DNA binding nucleus YBR232C_d YBR232C hom 0.0128072923463554 0.458474365872368 1857 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL232W TAN1 hom FT RNA PROCESSING|NUCLEUS 0.0127943200670975 0.458929707819199 1858 Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress tRNA modification RNA binding nucleus|cytoplasm YKR093W PTR2 hom FF|FT PROTEIN LOCALIZATION|PLASMA MEMBRANE 0.0127401151041618 0.460835102665697 1859 Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p dipeptide transport|tripeptide transport|peptide transport dipeptide transporter activity|tripeptide transporter activity|peptide transporter activity integral to membrane|plasma membrane YPL049C DIG1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0127196038541434 0.461557258532746 1860 MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown DNA binding|transcription factor binding nucleus YPL142C_d YPL142C het 0.0126984925231305 0.462970000914154 1861 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Unknown Unknown Unknown YHR034C PIH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0126861234860494 0.462870961516195 1862 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|rRNA processing|regulation of cell size|box C/D snoRNP assembly molecular_function R2TP complex|nucleus|small nucleolar ribonucleoprotein complex|cytoplasm YPL157W TGS1 hom FT CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0126826882199773 0.462858566945948 1863 Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs meiosis|RNA methylation|nucleologenesis|7-methylguanosine cap hypermethylation|regulation of telomere maintenance via telomerase RNA methyltransferase activity nucleolus YNL096C RPS7B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.0126754959217054 0.463112339557968 1864 Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosome biogenesis structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit YPR108W-A_p YPR108W-A hom 0.0126742306524701 0.463357292957567 1865 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YJL015C_d YJL015C het 0.0126731569819317 0.463395177126865 1866 Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Unknown Unknown Unknown YMR057C_d YMR057C hom 0.0126041674544715 0.465633281306862 1867 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Unknown Unknown Unknown YGR238C KEL2 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH 0.0125997294963016 0.465790382245411 1868 Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate conjugation with cellular fusion|negative regulation of exit from mitosis molecular_function mating projection tip|cellular bud neck|cellular bud tip YHR203C RPS4B hom FT TRANSLATION|RIBOSOME 0.0125907489532954 0.466108377859845 1869 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YCR044C PER1 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0125902111815506 0.466260530229181 1870 Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 GPI anchor biosynthetic process|cellular manganese ion homeostasis molecular_function fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum YNR043W MVD1 het FT LIPID METABOLISM 0.0125854880289649 0.466294719974691 1871 Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer sterol biosynthetic process diphosphomevalonate decarboxylase activity cytoplasm YLR342W FKS1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE|CYTOSKELETON 0.0125797266148852 0.466498836868707 1872 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling (1->3)-beta-D-glucan biosynthetic process|positive regulation of endocytosis|regulation of cell size 1,3-beta-D-glucan synthase activity mitochondrion|1,3-beta-D-glucan synthase complex|integral to membrane|plasma membrane|cellular bud neck|cellular bud tip|actin cortical patch YGR058W PEF1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0125724766085891 0.4667557624173 1873 Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly bipolar cellular bud site selection|regulation of COPII vesicle coating|axial cellular bud site selection zinc ion binding|calcium ion binding cytosol|site of polarized growth|nucleus|cellular bud neck|cytoplasm YNL093W YPT53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.0125652295503791 0.467012661898117 1874 Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication endocytosis|protein targeting to vacuole GTPase activity late endosome YOR102W_d YOR102W het 0.0125629819143701 0.467092353809265 1875 Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Unknown Unknown Unknown YPL143W RPL33A het FT TRANSLATION|RIBOSOME 0.012526991034139 0.469034027351294 1876 Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YIL168W YIL168W hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.012518653684356 0.468798338345134 1877 Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase serine family amino acid metabolic process L-serine ammonia-lyase activity cellular_component YGR184C UBR1 hom FT PROTEOLYSIS 0.0125045464681066 0.469897351918902 1878 E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) regulation of dipeptide transport|ubiquitin-dependent protein catabolic process via the N-end rule pathway|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process|protein polyubiquitination ubiquitin-protein ligase activity proteasome regulatory particle, base subcomplex|cytoplasm YNL220W ADE12 hom FT NUCLEOTIDE METABOLISM 0.012487533312033 0.46977181959903 1879 Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence purine nucleotide biosynthetic process DNA replication origin binding|adenylosuccinate synthase activity cytoplasm YPR063C_p YPR063C hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0124803012271009 0.47009537335915 1880 ER-localized protein of unknown function biological_process molecular_function endoplasmic reticulum YEL034W HYP2 het FF|FT KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION|RIBOSOME 0.0124527843073419 0.471207382747384 1881 Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication positive regulation of translational initiation|translational frameshifting|positive regulation of translational termination|positive regulation of translational elongation ribosome binding|RNA binding|translation elongation factor activity|translation initiation factor activity mitochondrion|cytosolic ribosome|cytoplasm YML127W RSC9 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0124307239451115 0.471794926081782 1882 Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|rRNA transcription|regulation of transcription from RNA polymerase II promoter|chromatin remodeling|transcription elongation from RNA polymerase II promoter|response to stress chromatin binding|DNA translocase activity RSC complex YNL332W THI12 hom 0.0124253756916275 0.472051765585021 1883 Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 thiamine biosynthetic process molecular_function cellular_component YOR027W STI1 hom 0.0123962899310637 0.473023529835254 1884 Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding Hsp70 protein binding|Hsp90 protein binding|mRNA binding|ATPase inhibitor activity cytoplasm YGL113W SLD3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0123938621511639 0.473110219256357 1885 Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress DNA unwinding involved in replication|double-strand break repair via break-induced replication|DNA replication initiation chromatin binding nuclear chromatin|DNA replication preinitiation complex YOL097C WRS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0123915110780637 0.4731941780139 1886 Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA tryptophanyl-tRNA aminoacylation tryptophan-tRNA ligase activity cytoplasm YEL035C_p UTR5 het 0.0123469535586184 0.475314739187639 1887 Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions biological_process molecular_function cellular_component YLR015W BRE2 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0123012168691022 0.476424851288895 1888 Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L histone H3-K4 methylation|telomere maintenance|histone H3-K4 trimethylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YKL114C APN1 hom FF|FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0122996642341542 0.476480509082767 1889 Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine base-excision repair 3'-tyrosyl-DNA phosphodiesterase activity|DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity mitochondrion|nucleus YPL206C PGC1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0122938373824362 0.476689417821497 1890 Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs glycerophospholipid catabolic process|phosphatidylglycerol catabolic process phosphatidylglycerol phospholipase C activity mitochondrion|lipid particle YML092C PRE8 het FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0122536998957141 0.478129819050013 1891 Alpha 2 subunit of the 20S proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YDL104C QRI7 hom FT RNA PROCESSING|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0121973636718468 0.480155544276408 1892 Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification mitochondrial tRNA threonylcarbamoyladenosine modification molecular_function mitochondrion YDR346C SVF1 hom FT RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0121862349256807 0.480556261681511 1893 Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance cellular response to oxidative stress molecular_function nucleus|cytoplasm YMR304W UBP15 hom FT PROTEOLYSIS 0.0121766825327732 0.481162345698115 1894 Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p protein deubiquitination ubiquitin-specific protease activity peroxisome|cytoplasm YBR024W SCO2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0121305518144625 0.482563999568496 1895 Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication copper ion transport thioredoxin peroxidase activity mitochondrial envelope|mitochondrion YPL031C PHO85 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES A 0.0121218692150396 0.482877473784594 1896 Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle regulation of transcription involved in G1/S phase of mitotic cell cycle|negative regulation of calcium-mediated signaling|regulation of protein stability|regulation of protein localization|protein phosphorylation|response to DNA damage stimulus|negative regulation of phosphate metabolic process|fungal-type cell wall organization|regulation of establishment or maintenance of cell polarity|negative regulation of glycogen biosynthetic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity|positive regulation of macroautophagy|negative regulation of macroautophagy protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YLR266C PDR8 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0120485628991417 0.485528507142674 1897 Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding nucleus YDR270W CCC2 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0120467252695403 0.485595063788738 1898 Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism cellular iron ion homeostasis|copper ion export|transmembrane transport copper ion binding|cation-transporting ATPase activity integral to membrane|trans-Golgi network transport vesicle membrane YOR124C UBP2 hom FT PROTEOLYSIS 0.0120190502597398 0.486598014660651 1899 Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity protein deubiquitination|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway ubiquitin-specific protease activity cytoplasm YBR078W ECM33 hom FF|FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0119949066036783 0.487473901380291 1900 GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p fungal-type cell wall organization molecular_function mitochondrion|fungal-type cell wall|plasma membrane YOR135C_d IRC14 hom 0.0119659126213701 0.488526872129889 1901 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YPL162C_p YPL162C hom 0.0119593909400526 0.488763887919984 1902 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function fungal-type vacuole membrane|integral to membrane YDR383C NKP1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0119475964691667 0.489192688604981 1903 Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 biological_process molecular_function kinetochore YGL117W_p YGL117W hom FF 0.0119322087372519 0.490141418015066 1904 Putative protein of unknown function biological_process molecular_function cellular_component YOR210W RPB10 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0119302830862629 0.489822501937812 1905 RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase II, core complex YML038C YMD8 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0119160219044593 0.490341611983658 1906 Putative nucleotide sugar transporter, has similarity to Vrg4p UDP-glucose transport|nucleotide-sugar transport nucleotide-sugar transmembrane transporter activity COPI-coated vesicle|integral to membrane YJL100W LSB6 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0118973991066981 0.491019930722264 1907 Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity fungal-type vacuole membrane|plasma membrane|cytoplasm YMR265C_p YMR265C hom 0.0118680104550281 0.492091410298273 1908 Putative protein of unknown function biological_process molecular_function cellular_component YDR129C SAC6 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.011865995385111 0.493330012748496 1909 Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress bipolar cellular bud site selection|actin filament organization|endocytosis|response to osmotic stress actin filament binding|protein binding, bridging actin filament bundle|mating projection tip|actin cortical patch YGR273C_p YGR273C hom 0.0118622859866065 0.492300264237921 1910 Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene biological_process molecular_function cellular_component YLR033W RSC58 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0118503124930551 0.493060211014075 1911 Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance nucleosome disassembly|transcription elongation from RNA polymerase II promoter|G1/S transition of mitotic cell cycle|chromatin remodeling molecular_function RSC complex YKL203C TOR2 het FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.0118432705263733 0.492994373323686 1912 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication G1 phase of mitotic cell cycle|regulation of cell cycle|actin filament reorganization involved in cell cycle|positive regulation of endocytosis|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|Rho protein signal transduction|ribosome biogenesis|signal transduction|TOR signaling cascade|cytoskeleton organization|negative regulation of autophagy protein binding|protein serine/threonine kinase activity TORC1 complex|TORC2 complex|mitochondrion|fungal-type vacuole membrane|extrinsic to internal side of plasma membrane|plasma membrane|cytoplasm YGR125W_p YGR125W hom 0.0117611681635944 0.495997310180151 1913 Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function integral to membrane|fungal-type vacuole YHR088W RPF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.011734247099356 0.497112540910355 1914 Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit assembly|ribosomal large subunit export from nucleus|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YKL133C_p YKL133C hom 0.0117135077477284 0.497744979841674 1915 Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) biological_process molecular_function cellular_component YOR185C GSP2 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS 0.0116781556118633 0.499235638683207 1916 GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress nucleus organization GTPase activity integral to membrane|nucleus YCR002C CDC10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0116488696106913 0.500120434101018 1917 Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress sporulation|maintenance of cell polarity|exit from mitosis|cell cycle cytokinesis|septin ring assembly protein complex scaffold|structural molecule activity|GTP binding|GTPase activity|1-phosphatidylinositol binding spindle microtubule|ascospore-type prospore|septin complex|prospore membrane|mating projection base|meiotic spindle|septin filament array|cellular bud neck septin ring|mating projection tip YFR044C DUG1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS|MITOCHONDRION 0.0115902128877211 0.502281263162069 1918 Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process metallodipeptidase activity|omega peptidase activity mitochondrion|ribosome|cytoplasm YPR089W_p YPR089W hom FT GOLGI APPARATUS 0.0115589966235924 0.503433234537102 1919 Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p biological_process molecular_function cellular_component YDL026W_d YDL026W hom 0.0115411805376622 0.504282295249595 1920 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR447C RPS17B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0115307096365335 0.504478310217258 1921 Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YHR157W REC104 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0115098701719206 0.505248964661215 1922 Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YKL038W RGT1 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0115045786516813 0.505444746512827 1923 Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication glucose transport|regulation of glucose import|negative regulation of transcription from RNA polymerase II promoter|glucose metabolic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nucleus YER124C DSE1 hom FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0114963372599789 0.505749750667048 1924 Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress cytokinesis, completion of separation|regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion|invasive growth in response to glucose limitation molecular_function cellular bud neck YLR176C RFX1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0114931014033329 0.505869532361326 1925 Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|cytoplasm YKR081C RPF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0114738173791941 0.506774027750434 1926 Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles ribosomal large subunit assembly|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 7S RNA binding|5S rRNA binding|rRNA binding nucleolus|preribosome, large subunit precursor YLR052W IES3 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0114351668414762 0.508143264916101 1927 Subunit of the INO80 chromatin remodeling complex nucleosome mobilization|telomere maintenance|chromatin silencing at telomere molecular_function nucleus|Ino80 complex YDR318W MCM21 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0114295048590761 0.50822671096127 1928 Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function COMA complex|condensed nuclear chromosome kinetochore YPR179C HDA3 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0113661559808182 0.510580422270799 1929 Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p negative regulation of transcription from RNA polymerase II promoter|chromosome segregation|gene silencing by RNA|gene silencing involved in chronological cell aging|histone deacetylation histone deacetylase activity|chromatin binding|DNA binding nucleus|HDA1 complex YLR255C_d YLR255C hom 0.0113448749879111 0.511372388280291 1930 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL197C_d YPL197C hom 0.0113410773760236 0.511892111481457 1931 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Unknown Unknown Unknown YLR113W HOG1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0112896995255077 0.513428709442088 1932 Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter by pheromones|protein phosphorylation|hyperosmotic response|response to arsenic-containing substance|osmosensory signaling pathway|positive regulation of transcription from RNA polymerase II promoter|cellular response to heat MAP kinase activity nucleus|cytoplasm YIL061C SNP1 het FT RNA PROCESSING|NUCLEUS 0.0112869944528711 0.513529634689209 1933 Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding|U1 snRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YKR031C SPO14 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|NUCLEUS 0.0112805611498575 0.513769700276387 1934 Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions phospholipid metabolic process|exocytosis|cell morphogenesis involved in conjugation with cellular fusion|ascospore-type prospore assembly phospholipase D activity|phosphatidylinositol-3-phosphate binding endosome|nucleus|prospore membrane YLR023C IZH3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.01127975340538 0.514051231175084 1935 Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity cellular zinc ion homeostasis metal ion binding integral to membrane|endoplasmic reticulum|membrane YJL133W MRS3 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0111640219358913 0.518128570961015 1936 Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication mitochondrial iron ion transport|RNA splicing iron ion transmembrane transporter activity mitochondrion|integral to membrane YMR086C-A_d YMR086C-A hom 0.0111383553162769 0.519091131510002 1937 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR318W EST2 hom FT NUCLEUS|CHROMOSOME 0.0110618569152693 0.522400565781021 1938 Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia telomere maintenance via telomerase|replicative cell aging telomerase activity|telomeric template RNA reverse transcriptase activity|telomeric DNA binding telomerase catalytic core complex|nucleolus|nucleus|telomerase holoenzyme complex YEL006W YEA6 hom FT MITOCHONDRION 0.0110548529554039 0.522229046359594 1939 Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog transport|NAD transmembrane transport transporter activity|NAD transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YKL004W AUR1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0110491771360577 0.522442689768075 1940 Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance inositolphosphoceramide metabolic process|sphingolipid biosynthetic process inositol phosphoceramide synthase activity integral to membrane|Golgi apparatus|inositol phosphoceramide synthase complex YDR363W ESC2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS 0.0109182452861165 0.527383524403264 1941 Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member intra-S DNA damage checkpoint|double-strand break repair via homologous recombination|chromatin silencing at silent mating-type cassette|mitotic sister chromatid cohesion molecular_function nucleus YCR098C GIT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0109088202711813 0.527740103064939 1942 Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability transmembrane transport|glycerophosphodiester transport glycerophosphodiester transmembrane transporter activity integral to membrane|plasma membrane YOR072W_d YOR072W hom FF 0.0108997876112627 0.528081952927684 1943 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Unknown Unknown Unknown YMR083W ADH3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0108004892754383 0.531847415911795 1944 Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production NADH oxidation|fermentation|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity mitochondrion|mitochondrial matrix YJL126W NIT2 hom 0.0107979575763144 0.531943597194538 1945 Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member biological_process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component YLR163C MAS1 het FF|FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0107826312350042 0.532832025908452 1946 Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion|mitochondrial processing peptidase complex YDR429C TIF35 het FF|FT TRANSLATION 0.0107686607978222 0.533057245648707 1947 eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity eukaryotic translation initiation factor 3 complex YCL036W GFD2 hom 0.0107341246008504 0.534371570260151 1948 Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component YIL138C TPM2 hom FT VESICLE-MEDIATED TRANSPORT|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0106396933729997 0.537973613511793 1949 Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication vacuole inheritance|exocytosis|intracellular mRNA localization|budding cell apical bud growth|budding cell isotropic bud growth|mitochondrion inheritance|actin polymerization or depolymerization|vesicle-mediated transport|actin filament organization|establishment of cell polarity|actin filament bundle assembly actin lateral binding cellular bud neck contractile ring|actin filament bundle YEL051W VMA8 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0106395623233018 0.537978620804987 1950 Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis vacuolar acidification proton-transporting ATPase activity, rotational mechanism fungal-type vacuole membrane|vacuolar proton-transporting V-type ATPase, V1 domain|integral to membrane YBL095W_p YBL095W hom FT MITOCHONDRION 0.0106231481850143 0.538605975361618 1951 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YPR051W MAK3 hom FT NUCLEUS 0.010556139898928 0.541170855638875 1952 Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatC complex YHR170W NMD3 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.0105308097830612 0.542866199293561 1953 Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex ribosomal large subunit export from nucleus ribosomal large subunit binding cytosol|cytosolic large ribosomal subunit YIL044C AGE2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.0105198624563954 0.542622231743864 1954 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport ARF GTPase activator activity clathrin-coated vesicle YJL153C INO1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM 0.0105121316082187 0.543280434319383 1955 Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element inositol biosynthetic process inositol-3-phosphate synthase activity cytoplasm YDR354W TRP4 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.010412883521085 0.547393713690169 1956 Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis tryptophan biosynthetic process anthranilate phosphoribosyltransferase activity nucleus|cytoplasm YER006W NUG1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0103833161702274 0.547814061204688 1957 GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus ribosomal large subunit export from nucleus|rRNA processing RNA binding|GTPase activity nucleolus|nucleus|preribosome, large subunit precursor YHR030C SLT2 hom FF|FT PROTEIN LOCALIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0103427715361329 0.550692286052838 1958 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway protein phosphorylation|endoplasmic reticulum unfolded protein response|signal transduction|UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|regulation of transcription factor import into nucleus|barrier septum assembly|response to acid|regulation of cell size MAP kinase activity|protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm|cellular bud tip YPL138C SPP1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0103372811779334 0.549590381549866 1959 Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein histone H3-K4 methylation|regulation of transcription, DNA-dependent|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific)|chromatin binding|methylated histone residue binding Set1C/COMPASS complex YGR134W CAF130 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0103284466982541 0.54993159580716 1960 Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex YOR168W GLN4 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0102454294013425 0.553143061291797 1961 Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions glutaminyl-tRNA aminoacylation glutamine-tRNA ligase activity cytosol|mitochondrion YLR286C CTS1 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0101807933577751 0.555649806980089 1962 Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p cytokinesis, completion of separation|chitin catabolic process endochitinase activity nuclear envelope|extracellular region|fungal-type cell wall|endoplasmic reticulum|cellular bud neck YGL075C MPS2 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0101772964477683 0.555785583721078 1963 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p mitotic anaphase|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton nuclear envelope|spindle pole body YBL093C ROX3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0101120869667293 0.558614236771233 1964 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme negative regulation of transcription from RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex|nucleus YIL103W DPH1 hom 0.0100814179656362 0.559749127858057 1965 Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YBR056W YBR056W hom FT CARBOHYDRATE METABOLISM 0.0100206198811427 0.562177521174304 1966 Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function cytoplasm YPR137W RRP9 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00994727005882184 0.564926652172642 1967 Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein rRNA modification|rRNA processing snoRNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|90S preribosome YDR041W RSM10 het FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00982378965697849 0.569594304585627 1968 Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YLR438C-A LSM3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00981747997964916 0.569842253045526 1969 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YDR166C SEC5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.00978711501683182 0.571036210046486 1970 Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Golgi to plasma membrane transport|endoplasmic reticulum inheritance|exocytosis|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|exocyst|cellular bud tip YKR106W GEX2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00972114094050936 0.573634419981757 1971 Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway glutathione transmembrane transport solute:hydrogen antiporter activity integral to membrane|plasma membrane|vacuolar membrane YKR052C MRS4 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00967458976047044 0.575471075344337 1972 Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication mitochondrial iron ion transport|RNA splicing iron ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YMR197C VTI1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00966248041262928 0.575949298866716 1973 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Golgi to vacuole transport|vacuole fusion, non-autophagic|intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to Golgi membrane YLR449W FPR4 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00964937331599628 0.576524043235705 1974 Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones histone peptidyl-prolyl isomerization|negative regulation of histone H3-K36 methylation|regulation of transcription by chromatin organization|chromatin silencing at rDNA|nucleosome assembly peptidyl-prolyl cis-trans isomerase activity|macrolide binding chromatin|nucleus YNL119W NCS2 hom FT RNA PROCESSING 0.00964581604849164 0.577120145160342 1975 Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA wobble position uridine thiolation|pseudohyphal growth|protein urmylation|invasive growth in response to glucose limitation molecular_function cytosol|cytoplasm YOR385W_p YOR385W hom 0.00962165737274505 0.577562866453928 1976 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm YDR458C HEH2 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.00960593782157947 0.578468521963781 1977 Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle biological_process molecular_function nuclear envelope YKL121W_p DGR2 hom 0.00960401804192743 0.578260734534011 1978 Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds biological_process molecular_function cellular_component YNL111C CYB5 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00959720795988516 0.578700384370959 1979 Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation sterol biosynthetic process electron carrier activity endoplasmic reticulum membrane YLR370C ARC18 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.00959621239680483 0.579364346005858 1980 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin filament organization|establishment of mitochondrion localization molecular_function mitochondrion|Arp2/3 protein complex YLR204W QRI5 hom FF|FT RNA PROCESSING|MITOCHONDRION 0.00953843714278148 0.580858793422771 1981 Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA mRNA processing molecular_function mitochondrion|integral to mitochondrial inner membrane YDR069C DOA4 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION 0.00951708839497328 0.581705724253661 1982 Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole protein deubiquitination|endocytosis|regulation of DNA replication|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|free ubiquitin chain depolymerization|ubiquitin homeostasis|ubiquitin-dependent protein catabolic process endopeptidase activity|ubiquitin-specific protease activity|ubiquitin thiolesterase activity endosome|proteasome complex|mitochondrion YLR051C FCF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00949429725735908 0.582666783667803 1983 Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YOR250C CLP1 het FT RNA PROCESSING|NUCLEUS 0.00949352158200461 0.582641317893452 1984 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation mRNA 3'-end processing|mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage factor complex YPR050C_d YPR050C hom 0.00945915428138972 0.584006943641367 1985 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Unknown Unknown Unknown YDR024W_d FYV1 hom 0.00944629258848685 0.584742753498459 1986 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Unknown Unknown Unknown YBR192W RIM2 het FF|FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.00944456933563453 0.584586943756807 1987 Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family pyrimidine nucleotide transport|mitochondrial genome maintenance|transmembrane transport pyrimidine nucleotide transmembrane transporter activity|transporter activity mitochondrion|integral to membrane YGL133W ITC1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.00942200621792575 0.585484739356547 1988 Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex negative regulation of transcription from RNA polymerase II promoter by pheromones|chromatin remodeling|chromatin silencing at telomere molecular_function chromatin accessibility complex YMR224C MRE11 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.00936557120052233 0.587733109261287 1989 Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress DNA repair|meiotic DNA double-strand break formation|double-strand break repair via nonhomologous end joining|base-excision repair|ascospore formation|meiotic DNA double-strand break processing|double-strand break repair via break-induced replication|regulation of transcription during meiosis 3'-5' exonuclease activity|protein complex scaffold|double-stranded telomeric DNA binding|adenylate kinase activity|telomeric DNA binding|single-stranded telomeric DNA binding|endodeoxyribonuclease activity|G-quadruplex DNA binding|endonuclease activity mitochondrion|nucleus|Mre11 complex YPR124W CTR1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00934639883029613 0.588497843505828 1990 High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress copper ion import copper uptake transmembrane transporter activity integral to membrane|plasma membrane YPR170C_d YPR170C hom 0.00933274743647443 0.589042640732964 1991 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Unknown Unknown Unknown YHR146W CRP1 hom FT NUCLEUS 0.00932940726676665 0.58917597500601 1992 Protein that binds to cruciform DNA structures biological_process DNA binding nucleus YJL056C ZAP1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00931930357155167 0.589801604305456 1993 Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity|RNA polymerase II regulatory region sequence-specific DNA binding|zinc ion binding nucleus YIL029C_p YIL029C hom 0.00928192165476137 0.591073028866794 1994 Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W biological_process molecular_function integral to membrane YLR076C_d YLR076C het 0.00909837184529995 0.599471177073826 1995 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Unknown Unknown Unknown YLR382C NAM2 hom FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00902407804651202 0.602074479330608 1996 Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Group I intron splicing|leucyl-tRNA aminoacylation|mitochondrial translation mRNA binding|leucine-tRNA ligase activity mitochondrion YOR116C RPO31 het FF|FT NUCLEUS 0.00899047334757867 0.602777411992559 1997 RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YGR083C GCD2 het FT TRANSLATION 0.00891240378341598 0.605930257334182 1998 Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YNL195C_p YNL195C hom FT MITOCHONDRION 0.00885186893190765 0.608380027580909 1999 Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YBL031W SHE1 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00882922366862278 0.609350993704739 2000 Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function mitotic spindle elongation molecular_function microtubule cytoskeleton|nuclear microtubule|cellular bud neck|astral microtubule|spindle YDL101C DUN1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00873344111912175 0.613185359161547 2001 Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair protein phosphorylation|replication fork protection|cell cycle checkpoint|DNA damage checkpoint protein kinase activity nucleus YMR038C CCS1 hom FF|FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.00873333452667352 0.614038524813736 2002 Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress intracellular copper ion transport superoxide dismutase copper chaperone activity cytosol|mitochondrial inner membrane|nucleus YDL066W IDP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.00870067721048285 0.61451774072657 2003 Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes isocitrate metabolic process|glutamate biosynthetic process isocitrate dehydrogenase (NADP+) activity mitochondrial nucleoid|mitochondrion YPL032C SVL3 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|SITE OF POLARIZED GROWTH 0.00866647577330428 0.616437126101903 2004 Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress endocytosis molecular_function cellular bud|cellular bud neck|cytoplasm YKR092C SRP40 hom FT NUCLEUS 0.00858500932037903 0.619231660399428 2005 Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 nucleocytoplasmic transport molecular_function nucleolus YBR081C SPT7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|HISTONE ACETYLTRANSFERASE 0.00858286128899161 0.619319350109606 2006 Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex conjugation with cellular fusion|histone acetylation|protein complex assembly|chromatin modification structural molecule activity SLIK (SAGA-like) complex|mitochondrion|SAGA complex YPR074C TKL1 hom FF|FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.00855805813700338 0.620332288381285 2007 Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication pentose-phosphate shunt transketolase activity cytoplasm YIL068C SEC6 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.00854721380448891 0.620827515998672 2008 Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p exocyst localization|Golgi to plasma membrane transport|exocytosis|vesicle fusion|negative regulation of SNARE complex assembly|vesicle docking involved in exocytosis protein homodimerization activity mating projection tip|cellular bud neck|cellular bud tip|exocyst YER068C-A_d YER068C-A hom 0.00853097794887452 0.621595231160437 2009 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR002W ALG6 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00837330989281547 0.62789979927727 2010 Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation|aerobic respiration transferase activity, transferring hexosyl groups|dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum YBL105C PKC1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00835108515555652 0.629068986691017 2011 Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) intracellular protein kinase cascade|regulation of nuclear-transcribed mRNA poly(A) tail shortening|fungal-type cell wall organization|signal transduction|actin filament organization|peroxisome degradation|protein phosphorylation|cytoplasmic mRNA processing body assembly protein kinase C activity site of polarized growth|cytoskeleton|nucleus|plasma membrane|cytoplasm YGR204W ADE3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00831683646915835 0.630220894058477 2012 Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine purine nucleobase biosynthetic process|folic acid-containing compound metabolic process methylenetetrahydrofolate dehydrogenase (NADP+) activity|formate-tetrahydrofolate ligase activity|methenyltetrahydrofolate cyclohydrolase activity nucleus|cytoplasm YCL016C DCC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|CHROMOSOME 0.00826641582289749 0.632296303922929 2013 Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance maintenance of DNA trinucleotide repeats|response to DNA damage stimulus|mitotic sister chromatid cohesion molecular_function Ctf18 RFC-like complex YMR253C_p YMR253C hom 0.00826471994920664 0.632366159787871 2014 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function integral to membrane|cytoplasm YML085C TUB1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.00825841167542156 0.632778399417504 2015 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|cytoplasmic microtubule|kinetochore microtubule|nuclear microtubule|spindle pole body|polar microtubule YGL092W NUP145 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00823255285525618 0.633691796443435 2016 Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 double-strand break repair|poly(A)+ mRNA export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|tRNA export from nucleus|protein targeting to nuclear inner membrane|telomere tethering at nuclear periphery|nuclear pore distribution|positive regulation of transcription, DNA-dependent|NLS-bearing substrate import into nucleus|protein import into nucleus nucleocytoplasmic transporter activity|RNA binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore|nuclear pore outer ring YGR174C CBP4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00804914484379699 0.641322357533731 2017 Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|integral to mitochondrial membrane YMR053C STB2 hom FT NUCLEUS|CHROMOSOME 0.00804614307419605 0.64154627759876 2018 Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p biological_process molecular_function Sin3-type complex YGR253C PUP2 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00804082726516961 0.641866032544866 2019 Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YDR064W RPS13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00801680882501368 0.642613039375956 2020 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome|SSU rRNA binding cytosolic small ribosomal subunit|90S preribosome YBL062W_d YBL062W hom 0.00800688845250962 0.643024511968267 2021 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR026C GCN3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.00800635820228157 0.643046508523584 2022 Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YBR004C GPI18 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00796907779580074 0.644593807703644 2023 Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria GPI anchor biosynthetic process mannosyltransferase activity|dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to endoplasmic reticulum membrane|mitochondrion|mannosyltransferase complex|integral to membrane|endoplasmic reticulum YKR024C DBP7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00791769930200093 0.646974524459253 2024 Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YPL102C_d YPL102C hom 0.00790902774484549 0.647089382693739 2025 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Unknown Unknown Unknown YOL121C RPS19A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.00787321576457225 0.648579558519625 2026 Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit biogenesis|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YPR161C SGV1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00785554816582655 0.649315245634382 2027 Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins phosphorylation of RNA polymerase II C-terminal domain|protein phosphorylation|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YEL050C RML2 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|TRANSLATION|MITOCHONDRION|RIBOSOME 0.00785483380006363 0.649344999359357 2028 Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor cytoplasmic translation|fatty acid metabolic process structural constituent of ribosome mitochondrial large ribosomal subunit YFL025C BST1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00784005685478174 0.650497355750945 2029 GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules protein retention in ER lumen|vesicle organization|ER-associated protein catabolic process|ER to Golgi vesicle-mediated transport|GPI anchor metabolic process phosphatidylinositol deacylase activity integral to membrane|endoplasmic reticulum YMR227C TAF7 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00767285876510346 0.65694246124972 2030 TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YER094C PUP3 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00763318783755755 0.658603474780339 2031 Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activator activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YBR194W AIM4 hom 0.00760835683351902 0.659882068692324 2032 Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress biological_process molecular_function cytoplasm YJL036W SNX4 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.00760106561803377 0.659949660678409 2033 Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p piecemeal microautophagy of nucleus|mitochondrion degradation|early endosome to Golgi transport|CVT pathway|protein targeting to vacuole phosphatidylinositol-3-phosphate binding cytosol|pre-autophagosomal structure|extrinsic to membrane|early endosome YGL012W ERG4 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.00759443236997492 0.660227785787228 2034 C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol ergosterol biosynthetic process delta24(24-1) sterol reductase activity integral to membrane|endoplasmic reticulum YOL068C HST1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00749099197563169 0.664570983741762 2035 NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance chromatin silencing|negative regulation of mitotic recombination|histone deacetylation NAD-independent histone deacetylase activity|NAD-dependent histone deacetylase activity Set3 complex YDL172C_d YDL172C hom 0.00734701292426711 0.670635056027522 2036 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL172W NUP49 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00733691968126726 0.671060971880477 2037 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) poly(A)+ mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|nuclear envelope|integral to membrane|nuclear pore YOL009C MDM12 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00732791995242967 0.671440832596941 2038 Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins mitochondrion inheritance|mitochondrion organization|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrion|ERMES complex YMR294W JNM1 hom FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00716318747787196 0.678408602296829 2039 Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B nuclear migration|establishment of mitotic spindle orientation structural constituent of cytoskeleton dynactin complex|cell cortex|spindle pole body|astral microtubule YPR039W_d YPR039W hom 0.00708918592224792 0.681547694877262 2040 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Unknown Unknown Unknown YNR042W_d YNR042W hom 0.00700536518247782 0.685109975164462 2041 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Unknown Unknown Unknown YER141W COX15 hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.00697974791514894 0.686200080426751 2042 Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase heme a biosynthetic process oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor mitochondrion|integral to membrane|mitochondrial inner membrane YML122C_d YML122C hom FF 0.00694823053824732 0.688072721950079 2043 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL085W EXO70 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0069151443725802 0.689172138155363 2044 Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Golgi to plasma membrane transport|exocytosis|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YDR497C ITR1 hom FT PLASMA MEMBRANE 0.0069107332351905 0.689140142637357 2045 Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress myo-inositol transport|transmembrane transport myo-inositol transmembrane transporter activity integral to membrane|plasma membrane|membrane YLR199C PBA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.00684870499935942 0.691786590196828 2046 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly proteasome assembly molecular_function cytoplasm YMR001C-A_p YMR001C-A hom 0.00662001981414866 0.701575821157819 2047 Putative protein of unknown function biological_process molecular_function cellular_component YOR322C LDB19 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|GOLGI APPARATUS|PLASMA MEMBRANE 0.00661961300237341 0.701593280151656 2048 Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye ubiquitin-dependent endocytosis ubiquitin protein ligase binding cytosol|Golgi apparatus|plasma membrane|clathrin-coated vesicle|cytoplasm YLR372W SUR4 hom FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.00656504851221895 0.703936428274474 2049 Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis post-Golgi vesicle-mediated transport|fatty acid elongation|fatty acid biosynthetic process|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum YKL019W RAM2 het FT PROTEIN PRENYLATION 0.00653001941402663 0.705442157119885 2050 Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor protein farnesylation|protein geranylgeranylation|peptide pheromone maturation protein geranylgeranyltransferase activity|protein farnesyltransferase activity|CAAX-protein geranylgeranyltransferase activity CAAX-protein geranylgeranyltransferase complex|protein farnesyltransferase complex YER041W YEN1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00649318574604499 0.707026700117926 2051 Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p DNA repair|response to DNA damage stimulus single-stranded DNA specific endodeoxyribonuclease activity|crossover junction endodeoxyribonuclease activity nucleus|cytoplasm YBR243C ALG7 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00641528379646802 0.71038212939394 2052 UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin protein N-linked glycosylation|aerobic respiration UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity UDP-N-acetylglucosamine transferase complex|integral to membrane YIL026C IRR1 het FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.00639970891741986 0.711259658092546 2053 Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability spore germination|fungal-type cell wall organization|cytogamy|mitotic sister chromatid cohesion chromatin binding nucleus|nuclear mitotic cohesin complex YDR535C_d YDR535C hom 0.00638106971188059 0.711857594411211 2054 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Unknown Unknown Unknown YKR001C VPS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|PEROXISOME ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.00636235595367573 0.713034135234413 2055 Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis vacuolar transport|peroxisome fission|endocytosis|actin cytoskeleton organization|protein retention in Golgi apparatus|peroxisome organization|lipid tube assembly|protein targeting to vacuole GTPase activity mitochondrial outer membrane|fungal-type vacuole membrane|peroxisome|actin cortical patch YGL155W CDC43 het FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS|PROTEIN PRENYLATION 0.00625415988621648 0.717339902809215 2056 Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis cellular calcium ion homeostasis|protein geranylgeranylation|establishment of cell polarity|small GTPase mediated signal transduction protein geranylgeranyltransferase activity|CAAX-protein geranylgeranyltransferase activity CAAX-protein geranylgeranyltransferase complex|intracellular YGL040C HEM2 het FT COFACTOR METABOLISM|NUCLEUS 0.00612871464695736 0.722773285362341 2057 Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus heme biosynthetic process porphobilinogen synthase activity nucleus|cytoplasm YDR187C_d YDR187C het FF 0.00610350233486129 0.72386699947916 2058 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Unknown Unknown Unknown YGR089W NNF2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00608621416632157 0.724617288965507 2059 Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation biological_process molecular_function integral to membrane YPL115C BEM3 hom FT SIGNALING|SITE OF POLARIZED GROWTH 0.00605958742598493 0.725773380328656 2060 Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly negative regulation of Rho protein signal transduction|establishment of cell polarity Rho GTPase activator activity|phosphatidylinositol-3-phosphate binding cell cortex|incipient cellular bud site|mating projection tip|cytoplasm|cellular bud tip YOR362C PRE10 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00603544384098692 0.726822197231726 2061 Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process mRNA binding mitochondrion|proteasome core complex, alpha-subunit complex|nucleus|proteasome storage granule|nuclear outer membrane-endoplasmic reticulum membrane network YPL169C MEX67 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0060213702867264 0.727590071975328 2062 Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP mRNA export from nucleus|ribosomal large subunit export from nucleus protein binding|RNA binding|structural constituent of nuclear pore integral to membrane|nuclear RNA export factor complex|nuclear pore|cytoplasm YLR338W_d OPI9 hom FF 0.00596562048391478 0.729858255380407 2063 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Unknown Unknown Unknown YER165W PAB1 het FF|FT RNA PROCESSING|TRANSLATION|RNA LOCALIZATION|NUCLEUS|RIBOSOME 0.00594082482854898 0.730937441766774 2064 Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G regulation of nuclear-transcribed mRNA poly(A) tail shortening|regulation of translational initiation poly(A) RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YNR035C ARC35 het FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00592614863042544 0.731769081214788 2065 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin mitochondrion inheritance|receptor-mediated endocytosis|microtubule cytoskeleton organization|actin cytoskeleton organization structural molecule activity cytosol|Arp2/3 protein complex YPL085W SEC16 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00590750357166194 0.732388527985462 2066 COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p COPII vesicle coating protein anchor ER to Golgi transport vesicle membrane YML017W PSP2 hom 0.00589393316545806 0.73297977146771 2067 Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process molecular_function cytoplasm YDR433W_d YDR433W hom 0.00582093659713113 0.736162838680638 2068 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL048C ELM1 hom FT CELL CYCLE|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00578095872183673 0.738058773433165 2069 Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring positive regulation of protein autophosphorylation|protein autophosphorylation|cell morphogenesis|budding cell bud growth|protein phosphorylation|response to drug|septin checkpoint|pseudohyphal growth|glucose metabolic process|axial cellular bud site selection|response to osmotic stress|cytokinesis protein serine/threonine kinase activity|protein kinase activity cellular bud neck contractile ring YBR021W FUR4 hom FF|FT PLASMA MEMBRANE 0.00575053351335252 0.739424677408138 2070 Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation uracil transport|transmembrane transport uracil:cation symporter activity integral to membrane|membrane raft|plasma membrane YNL123W NMA111 hom FT LIPID METABOLISM|PROTEOLYSIS|NUCLEUS 0.00557525105199854 0.746909141161259 2071 Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases apoptotic process|cellular lipid metabolic process|protein catabolic process|cellular response to heat serine-type peptidase activity nucleus YPR024W YME1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00551522455392844 0.749686831788721 2072 Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover chronological cell aging|misfolded or incompletely synthesized protein catabolic process|mitochondrion organization ATP-dependent peptidase activity mitochondrion|mitochondrial inner membrane|i-AAA complex YDR297W SUR2 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.005464616609954 0.751764550866102 2073 Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis sphingolipid metabolic process sphingosine hydroxylase activity integral to membrane|endoplasmic reticulum membrane YNL299W TRF5 hom FT CELL CYCLE|RNA PROCESSING|CELL DIVISION|NUCLEUS 0.00514512355901446 0.765840738911199 2074 Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|histone mRNA catabolic process|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|snoRNA polyadenylation polynucleotide adenylyltransferase activity nucleolus|TRAMP complex YGR229C SMI1 hom FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|NUCLEUS|SITE OF POLARIZED GROWTH 0.00508041221544367 0.76870138962682 2075 Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity regulation of mitotic cell cycle|regulation of fungal-type cell wall biogenesis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip YML124C TUB3 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.00507186641373053 0.769112889103675 2076 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|cytoplasmic microtubule|kinetochore microtubule|nuclear microtubule|spindle pole body|polar microtubule YML060W OGG1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.00506982065664251 0.769169904612735 2077 Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance DNA repair|base-excision repair, AP site formation oxidized purine nucleobase lesion DNA N-glycosylase activity mitochondrion YKL013C ARC19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00506911153709024 0.769201275352308 2078 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin cortical patch assembly structural molecule activity Arp2/3 protein complex YFR002W NIC96 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00505209781467759 0.76995405853184 2079 Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization structural constituent of nuclear pore integral to membrane|nuclear pore nuclear basket|nuclear pore linkers|nuclear pore YNL229C URE2 hom FF|FT PROTEIN LOCALIZATION 0.0050176563809131 0.771478604856416 2080 Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion regulation of nitrogen utilization|response to aluminum ion|protein urmylation|cytoplasmic sequestering of transcription factor glutathione peroxidase activity|phosphoprotein binding|transcription corepressor activity cytosol YIL146C ATG32 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.00486890665890792 0.778073062282279 2081 Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane fungal-type cell wall organization|mitochondrion degradation molecular_function integral to mitochondrial outer membrane YJL095W BCK1 hom FF|FT SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH 0.00470143425129104 0.78551668683671 2082 Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p intracellular protein kinase cascade|endoplasmic reticulum unfolded protein response|peroxisome degradation|establishment of cell polarity|protein phosphorylation|response to acid|response to nutrient protein kinase activity|MAP kinase kinase kinase activity intracellular|mating projection tip YOR211C MGM1 hom FT MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00468070919332282 0.786439230603965 2083 Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy mitochondrion organization|mitochondrial genome maintenance|mitochondrial fusion GTPase activity intrinsic to mitochondrial inner membrane|extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial inner boundary membrane|mitochondrial crista|mitochondrial intermembrane space YPL195W APL5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00459703169141813 0.790167040475547 2084 Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function vesicle-mediated transport|Golgi to vacuole transport protein binding AP-3 adaptor complex YER135C_d YER135C hom 0.00456727105860126 0.791494031257966 2085 Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Unknown Unknown Unknown YNL052W COX5A hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00454472495168192 0.792499737472733 2086 Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YMR211W DML1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00447676018652901 0.795533499849004 2087 Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family plasmid maintenance|mitochondrial genome maintenance molecular_function mitochondrion|cytoplasm YDL059C RAD59 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00439626820652974 0.799218464916242 2088 Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p DNA strand renaturation|telomere maintenance via recombination|double-strand break repair via break-induced replication|double-strand break repair via single-strand annealing protein binding nucleus YDR477W SNF1 hom FT KETONE METABOLISM|SIGNALING|TRANSLATION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00419698324250022 0.808054073375922 2089 AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation negative regulation of translation|protein phosphorylation|replicative cell aging|signal transduction|biofilm formation|positive regulation of gluconeogenesis|pseudohyphal growth|cell adhesion|regulation of carbohydrate metabolic process|invasive growth in response to glucose limitation|cellular response to nitrogen starvation AMP-activated protein kinase activity|protein kinase activity mitochondrion|AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|fungal-type vacuole|cytoplasm YOR370C MRS6 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|PROTEIN PRENYLATION 0.00401631634676968 0.816165684649523 2090 Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport|activation of Rab GTPase activity Rab geranylgeranyltransferase activity|Rab GTPase binding Rab-protein geranylgeranyltransferase complex|membrane|cytoplasm YLL009C COX17 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.00384974917111209 0.823661749567944 2091 Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex IV assembly|intracellular copper ion transport copper chaperone activity cytosol|mitochondrion|mitochondrial intermembrane space YCR046C IMG1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00382987030096523 0.824686280511706 2092 Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YBL007C SLA1 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00377964144896763 0.827178148205624 2093 Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains fungal-type cell wall organization|endocytosis|actin cortical patch assembly ubiquitin binding|protein binding, bridging cell cortex|nucleus|mating projection tip|actin cortical patch YKL143W LTV1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.00374979053995416 0.828167993010936 2094 Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature ribosomal small subunit biogenesis|cellular response to oxidative stress|response to osmotic stress|ribosomal small subunit export from nucleus molecular_function preribosome, small subunit precursor|EGO complex|nucleus|late endosome membrane|cytoplasm YKL211C TRP3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00373553320740256 0.829214115190336 2095 Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p tryptophan biosynthetic process indole-3-glycerol-phosphate synthase activity|anthranilate synthase activity anthranilate synthase complex|cytoplasm YJL014W CCT3 het FF 0.0036724476254912 0.831757600759457 2096 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YJL102W MEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.00358291927479589 0.835703270809858 2097 Mitochondrial elongation factor involved in translational elongation translational elongation translation elongation factor activity mitochondrion YPL139C UME1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0035152531250275 0.838763161186653 2098 Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of transcription from RNA polymerase I promoter|negative regulation of chromatin silencing at telomere|positive regulation of transcription from RNA polymerase II promoter|regulation of meiosis transcription corepressor activity Rpd3S complex|Rpd3L-Expanded complex|nucleus|Rpd3L complex YOL020W TAT2 hom FT PLASMA MEMBRANE 0.00348920222178566 0.839988978762656 2099 High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance aromatic amino acid transport|tryptophan transport high-affinity tryptophan transmembrane transporter activity|aromatic amino acid transmembrane transporter activity integral to membrane|plasma membrane YGR217W CCH1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00346882591646871 0.840864031410098 2100 Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together calcium ion transport voltage-gated calcium channel activity|calcium channel activity integral to membrane|plasma membrane YMR004W MVP1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|NUCLEUS 0.0034342453651074 0.842429567743264 2101 Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress protein targeting to vacuole phosphatidylinositol-3-phosphate binding nucleus|cytoplasm YHR143W-A RPC10 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00330261757922071 0.848394287534545 2102 RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YBL058W SHP1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.00327411993464385 0.849797679382401 2103 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate glycogen metabolic process|proteasomal ubiquitin-dependent protein catabolic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|ascospore formation|sister chromatid biorientation|autophagic vacuole assembly protein phosphatase type 1 regulator activity nucleus|cytoplasm YBL045C COR1 hom FT OXIDATION-REDUCTION PROCESS|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00326562542574786 0.850072167557146 2104 Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YMR058W FET3 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00315980071002762 0.854875860166407 2105 Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress iron assimilation by reduction and transport|high-affinity iron ion transport|response to copper ion ferroxidase activity|iron ion transmembrane transporter activity integral to membrane|high affinity iron permease complex|plasma membrane YNL064C YDJ1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION 0.00312020926553614 0.856674424238503 2106 Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family protein refolding|ER-associated protein catabolic process|protein targeting to mitochondrion|protein targeting to ER|'de novo' protein folding unfolded protein binding|ATPase activator activity cytosol|TRC complex|perinuclear region of cytoplasm YKL096C-B_p YKL096C-B hom 0.00307026835003196 0.858944205481109 2107 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YNL243W SLA2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00286181961967734 0.868430439498576 2108 Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo bipolar cellular bud site selection|fungal-type cell wall organization|actin filament organization|endocytosis|exocytosis protein binding, bridging incipient cellular bud site|mating projection tip|actin cortical patch YMR242C RPL20A hom FF|FT TRANSLATION|RIBOSOME 0.00273174339490624 0.874378334662638 2109 Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YER014C-A BUD25 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION 0.00269033474459101 0.876248778450561 2110 Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cellular bud site selection molecular_function cellular_component YPR036W VMA13 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.00261543643100456 0.879667380045885 2111 Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V1 domain|fungal-type vacuole membrane|integral to membrane YLR241W_p YLR241W hom 0.00258591558436481 0.881033052344629 2112 Putative protein of unknown function, may be involved in detoxification biological_process molecular_function integral to membrane YDR452W PPN1 hom FT NUCLEUS 0.0025698735635637 0.881748118395434 2113 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress polyphosphate catabolic process|polyphosphate metabolic process endopolyphosphatase activity|exopolyphosphatase activity fungal-type vacuole membrane|nucleus YMR216C SKY1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.00250994417800662 0.884486178873868 2114 SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 cellular cation homeostasis|cellular ion homeostasis|response to drug|protein phosphorylation|positive regulation of protein import into nucleus|regulation of cell size|mRNA splice site selection protein serine/threonine kinase activity|protein kinase activity cytoplasm YLR233C EST1 hom FT NUCLEUS|CHROMOSOME 0.00242843683032193 0.888212303719576 2115 TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA telomere maintenance via telomerase|telomere maintenance|G-quadruplex DNA formation single-stranded DNA binding|RNA binding|telomeric DNA binding nucleolus|nucleus|telomerase holoenzyme complex YNL283C WSC2 hom FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00239746765152552 0.889628718136436 2116 Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response fungal-type cell wall organization|response to heat|Rho protein signal transduction transmembrane signaling receptor activity site of polarized growth|cellular bud|mating projection tip|cytoplasm YIL021W RPB3 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00224190886772985 0.896809939705864 2117 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity nucleoplasm|DNA-directed RNA polymerase II, core complex YKL033W TTI1 het FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.00218282327727932 0.899470113332806 2118 Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ASTRA complex|mitochondrion|cytoplasm YMR040W YET2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00214263478656967 0.901311441249808 2119 Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function ribosome|integral to membrane YGR121C MEP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0020898675563131 0.903715597826103 2120 Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YGL168W HUR1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS 0.00207983349438308 0.904175653802757 2121 Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene DNA replication molecular_function cellular_component YOL004W SIN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL DIVISION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00207903524741425 0.904212254350613 2122 Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at rDNA|histone deacetylation|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of transcription from RNA polymerase I promoter|negative regulation of transcription during meiosis|regulation of DNA-dependent DNA replication initiation|negative regulation of transcription from RNA polymerase II promoter|negative regulation of chromatin silencing at telomere|double-strand break repair via nonhomologous end joining|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription involved in G2/M-phase of mitotic cell cycle transcription coactivator activity|transcription corepressor activity Sin3-type complex|Rpd3S complex|Rpd3L-Expanded complex|Rpd3L complex YLR273C PIG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.00203755222761319 0.906114577018397 2123 Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase regulation of glycogen biosynthetic process protein phosphatase type 1 regulator activity protein phosphatase type 1 complex YCR030C SYP1 hom FT CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.00201101565277399 0.907331770786785 2124 Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization septin cytoskeleton organization|actin cortical patch assembly molecular_function mating projection base|mating projection tip|cellular bud neck|cellular bud tip YDR532C KRE28 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.00199130262776102 0.908236119326593 2125 Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components biological_process molecular_function chromosome, centromeric region|condensed nuclear chromosome kinetochore|spindle pole body YML054C CYB2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00198431761240307 0.908556590284483 2126 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions lactate metabolic process L-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial intermembrane space YJL117W PHO86 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.00195578602997264 0.909865766901728 2127 Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress protein folding|ER to Golgi vesicle-mediated transport|regulation of phosphate transport unfolded protein binding endoplasmic reticulum YOR323C PRO2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00192729835724447 0.911384642448525 2128 Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis proline biosynthetic process glutamate-5-semialdehyde dehydrogenase activity nucleus|cytoplasm YDR382W RPP2B hom FT TRANSLATION|RIBOSOME 0.00190648548435654 0.912128505663454 2129 Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJL115W ASF1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.00190033942709938 0.912632239084472 2130 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition DNA replication-independent nucleosome assembly|histone acetylation|nucleosome disassembly|histone exchange|positive regulation of histone acetylation|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere|DNA replication-dependent nucleosome assembly|regulation of transcription from RNA polymerase II promoter in response to stress histone binding chromatin assembly complex YNL126W SPC98 het FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.00186647562376889 0.913965355922916 2131 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation|mitotic spindle organization in nucleus structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YDL109C_p YDL109C hom FT LIPID METABOLISM 0.00177668341544299 0.918089368620027 2132 Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication cellular lipid metabolic process molecular_function cellular_component YHR072W ERG7 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.00176177373023397 0.918774362972943 2133 Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis ergosterol biosynthetic process lanosterol synthase activity endoplasmic reticulum|lipid particle|plasma membrane YDL032W_d YDL032W hom 0.00166146184784283 0.923384524486982 2134 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Unknown Unknown Unknown YCR051W_p YCR051W hom FT NUCLEUS 0.00160828738549228 0.925829384903627 2135 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene biological_process molecular_function nucleus|cytoplasm YLR084C RAX2 hom FT CELL CYCLE|CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00156472360368687 0.927843628473614 2136 N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cellular bud site selection molecular_function mitochondrion|cellular bud scar|cellular bud neck|membrane YBR013C_p YBR013C hom 0.0015552893080786 0.928266832345562 2137 Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YDR048C_d YDR048C hom 0.00153930854988091 0.929001940366013 2138 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFR003C YPI1 het FT CELL CYCLE|LIPID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00151149524897501 0.930281484334988 2139 Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance negative regulation of phosphoprotein phosphatase activity|glycogen metabolic process|cellular ion homeostasis|Unknown|positive regulation of phosphoprotein phosphatase activity|negative regulation of protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint protein serine/threonine phosphatase inhibitor activity|protein phosphatase type 1 activator activity nucleus YJL175W_d YJL175W hom 0.00147296313783511 0.932054441483058 2140 Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Unknown Unknown Unknown YDR107C TMN2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.00144378347973571 0.933397294002316 2141 Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments cellular copper ion homeostasis|pseudohyphal growth|vacuolar transport|invasive growth in response to glucose limitation molecular_function fungal-type vacuole membrane|integral to membrane YPL041C_p YPL041C hom 0.00143111732240492 0.934049095958767 2142 Protein of unknown function involved in maintenance of proper telomere length biological_process molecular_function cellular_component YBL017C PEP1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.00139125179386833 0.935815285612879 2143 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments vacuolar transport|protein targeting to vacuole signal sequence binding integral to membrane|Golgi apparatus YCR021C HSP30 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00138295639882707 0.936197170715841 2144 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase negative regulation of ATPase activity|response to stress molecular_function integral to membrane|plasma membrane YIL042C PKP1 hom FT SIGNALING|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.00128808094539402 0.940601282222461 2145 Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p peptidyl-serine phosphorylation|protein phosphorylation|carbon utilization protein kinase activity|pyruvate dehydrogenase (acetyl-transferring) kinase activity mitochondrion YGR110W CLD1 hom FT LIPID METABOLISM|MITOCHONDRION 0.00127423097352269 0.941221275179903 2146 Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 cardiolipin acyl-chain remodeling|cardiolipin metabolic process phospholipase A2 activity mitochondrion YDR165W T