Strain Gene Zyg FClass FTerms similarity score p-value rank description GOBP GOMF GOCC YIL032C_d YIL032C hom 1 0 1 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR149C SKG6 hom FT SITE OF POLARIZED GROWTH 0.215053314405129 2.17754784920214e-36 2 Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p biological_process molecular_function cell cortex|incipient cellular bud site|integral to membrane|cellular bud neck|cellular bud tip YPL035C_d YPL035C hom 0.159409920674047 1.67735503460418e-20 3 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Unknown Unknown Unknown YEL017C-A PMP2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.150674553759829 1.93159906197347e-18 4 Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication cation transport molecular_function membrane YHL010C ETP1 hom 0.133942567649178 6.73580278977267e-15 5 Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences response to ethanol ubiquitin binding|nuclear localization sequence binding cellular_component YEL064C AVT2 hom FT ENDOPLASMIC RETICULUM 0.120372145395768 2.77196481197447e-12 6 Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters transport transporter activity integral to membrane|endoplasmic reticulum YPL262W FUM1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.116222322160292 1.58212082490887e-11 7 Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria fumarate metabolic process|tricarboxylic acid cycle fumarate hydratase activity cytosol|mitochondrion|mitochondrial matrix YGL002W ERP6 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.113035969439148 5.37915505571858e-11 8 Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|integral to membrane YNR004W_p SWM2 hom FT NUCLEUS 0.112574225982466 6.23659243724970e-11 9 Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds biological_process molecular_function nucleolus YBL008W-A_p YBL008W-A hom 0.110708019396383 1.33494219692219e-10 10 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YNL085W MKT1 hom FT RESPONSE TO DNA DAMAGE|TRANSLATION 0.107691056953288 4.0352867995624e-10 11 Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress positive regulation of translation|interspecies interaction between organisms|response to DNA damage stimulus nuclease activity polysome|cytoplasm YJR149W_p YJR149W hom FT OXIDATION-REDUCTION PROCESS 0.107140511489512 5.04427482268127e-10 12 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YPL211W NIP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.104842764576716 1.15512470832956e-09 13 Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p ribosomal large subunit assembly|ribosomal large subunit biogenesis|rRNA processing molecular_function cytosolic large ribosomal subunit|nucleolus|preribosome, large subunit precursor YDR029W_d YDR029W hom 0.103619395443764 2.06677868289842e-09 14 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR077W RTS2 het FT NUCLEUS 0.102619934472712 2.57510166745078e-09 15 Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication biological_process molecular_function nucleus|cytoplasm YPL052W OAZ1 hom FT TRANSLATION|PROTEOLYSIS 0.102528312248208 2.73382947400429e-09 16 Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Unknown|regulation of proteasomal protein catabolic process ornithine decarboxylase inhibitor activity cytoplasm YNR034W SOL1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.101306087298656 4.10369337347826e-09 17 Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress tRNA export from nucleus molecular_function|6-phosphogluconolactonase activity nucleus|cytoplasm YER099C PRS2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0993031314849193 8.60092593352991e-09 18 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YDR155C CPR1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0986494159100011 1.08228349475858e-08 19 Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress cellular protein metabolic process|ascospore formation|positive regulation of meiosis|histone deacetylation peptidyl-prolyl cis-trans isomerase activity|cyclosporin A binding histone deacetylase complex|mitochondrion|Set3 complex|nucleus YDR401W_d YDR401W hom 0.0966597950330342 2.03522457857365e-08 20 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL197C_d YPL197C hom 0.095033029057266 3.58764305884846e-08 21 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Unknown Unknown Unknown YIL092W_p YIL092W hom FT NUCLEUS 0.0941563624862516 4.68014059851345e-08 22 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YLR231C BNA5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0938216597106373 5.22298849689193e-08 23 Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p de novo NAD biosynthetic process from tryptophan kynureninase activity nucleus|cytoplasm YKR079C TRZ1 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0929596779297802 7.07359369183188e-08 24 tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 tRNA 3'-trailer cleavage, endonucleolytic 3'-tRNA processing endoribonuclease activity mitochondrion|nucleus|cytoplasm YJL065C DLS1 hom FT GENE SILENCING|NUCLEUS 0.091592175530957 1.09344565238425e-07 25 Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication chromatin silencing at telomere molecular_function chromatin accessibility complex|nucleus YFL022C FRS2 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0901504408517189 1.69828397490732e-07 26 Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar phenylalanyl-tRNA aminoacylation phenylalanine-tRNA ligase activity phenylalanine-tRNA ligase complex|cytoplasm YJL085W EXO70 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.089373513252983 2.21253202056528e-07 27 Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Golgi to plasma membrane transport|exocytosis|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YPL273W SAM4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.088266782887478 3.05619116377599e-07 28 S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio homocysteine metabolic process|L-methionine biosynthetic process from S-adenosylmethionine|S-adenosylhomocysteine metabolic process homocysteine S-methyltransferase activity nucleus|cytoplasm YOR380W RDR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0875819686297405 3.84894305763185e-07 29 Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to xenobiotic stimulus sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YEL055C POL5 het FT NUCLEUS 0.08722213053481 4.21196503499295e-07 30 DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA rRNA transcription|DNA replication DNA-directed DNA polymerase activity nucleolus YFR022W ROG3 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0870509971461285 4.52602367558576e-07 31 Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication ubiquitin-dependent endocytosis ubiquitin protein ligase binding cellular_component YBR104W YMC2 hom FT MITOCHONDRION 0.0867362823971153 4.88355488498346e-07 32 Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication mitochondrial transport|transmembrane transport organic acid transmembrane transporter activity mitochondrion|integral to membrane YDR250C_d YDR250C hom 0.0861654781955946 5.80480557112278e-07 33 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YKR064W OAF3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.086019996891492 6.376661563628e-07 34 Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|cellular response to oleic acid RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity mitochondrion|nucleus|cytoplasm YNL261W ORC5 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.0860031568876039 6.16683302693563e-07 35 Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing regulation of chromatin silencing at telomere|DNA replication initiation|pre-replicative complex assembly|chromatin silencing at silent mating-type cassette DNA replication origin binding|ATP binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YCR069W CPR4 hom 0.0859133928831153 6.26301422202824e-07 36 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity membrane YGR070W ROM1 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.0853109347061127 7.64718902123028e-07 37 GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP small GTPase mediated signal transduction|activation of Rho GTPase activity Rho guanyl-nucleotide exchange factor activity intracellular YNL181W_p YNL181W het FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0841252539276528 1.07501363330843e-06 38 Putative oxidoreductase; required for cell viability biological_process oxidoreductase activity|oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor nuclear envelope|endoplasmic reticulum YMR026C PEX12 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0840223376920323 1.13286618310999e-06 39 C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder protein import into peroxisome matrix ubiquitin-protein ligase activity|protein binding integral to peroxisomal membrane YDR329C PEX3 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOPLASMIC RETICULUM 0.0837272162929436 1.20857618405052e-06 40 Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization protein binding peroxisomal membrane|endoplasmic reticulum YLR084C RAX2 hom FT CELL CYCLE|CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.083539747692257 1.27223823177472e-06 41 N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cellular bud site selection molecular_function mitochondrion|cellular bud scar|cellular bud neck|membrane YKL042W SPC42 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0833459268518397 1.36616279299988e-06 42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane microtubule nucleation|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton central plaque of spindle pole body|intermediate layer of spindle pole body YBR214W SDS24 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION 0.0833285145052184 1.34847983866683e-06 43 Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication endocytosis|cytokinetic cell separation molecular_function cytoplasm YJL043W_p YJL043W hom FT MITOCHONDRION 0.0825628637437729 1.68521457859698e-06 44 Putative protein of unknown function; YJL043W is a non-essential gene biological_process molecular_function mitochondrion|cytoplasm YDR158W HOM2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0817524393254625 2.2202727713674e-06 45 Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis methionine biosynthetic process|threonine biosynthetic process|homoserine biosynthetic process aspartate-semialdehyde dehydrogenase activity nucleus|plasma membrane|cytoplasm YDL161W ENT1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0816389709327113 2.2150273864087e-06 46 Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress actin filament organization|endocytosis|actin cortical patch assembly clathrin binding mating projection tip|actin cortical patch YOL035C_d YOL035C hom 0.0813297041443675 2.40331771343307e-06 47 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR182C-A_p YBR182C-A hom 0.0804253705334093 3.1609782665469e-06 48 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YNL286W CUS2 hom FT RNA PROCESSING|NUCLEUS 0.0794724202858704 4.14469159188701e-06 49 Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) mRNA splicing, via spliceosome RNA binding U2 snRNP YDR531W CAB1 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0783545539049556 5.54332982457596e-06 50 Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element coenzyme A biosynthetic process pantothenate kinase activity nucleus|cytoplasm YNL124W NAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0767269457282497 8.64939247430304e-06 51 RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p box H/ACA snoRNP assembly RNA binding nucleoplasm|nucleus YGL175C SAE2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.076039010312324 1.05708378546181e-05 52 Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents DNA double-strand break processing|DNA catabolic process, endonucleolytic|meiotic DNA double-strand break formation|meiotic DNA double-strand break processing|gene conversion at mating-type locus, DNA double-strand break processing double-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleus|cytoplasm YBR149W ARA1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM 0.0756328751381332 1.16076165931826e-05 53 NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product cellular carbohydrate metabolic process alditol:NADP+ 1-oxidoreductase activity|D-arabinose 1-dehydrogenase [NAD(P)+] activity cytosol YJL144W YJL144W hom 0.0755423968996852 1.18913783580592e-05 54 Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress cellular response to water deprivation molecular_function cytoplasm YPL075W GCR1 het FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0751990181695795 1.30296935322888e-05 55 Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p negative regulation of transcription from RNA polymerase II promoter by glucose|nucleosome organization|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from a mobile element promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II distal enhancer sequence-specific DNA binding|RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nuclear envelope YOR004W UTP23 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0740864174752218 1.7475283047443e-05 56 Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|mitochondrion|nucleolus YER125W RSP5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGAN 0.0740720307364435 1.75412786852976e-05 57 E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p cellular response to UV|late endosome to vacuole transport via multivesicular body sorting pathway|chromatin assembly or disassembly|response to drug|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent endocytosis|protein monoubiquitination|positive regulation of fatty acid biosynthetic process|positive regulation of receptor-mediated endocytosis|regulation of phosphate metabolic process|regulation of ubiquinone biosynthetic process|regulation of dolichol biosynthetic process|regulation of multivesicular body size|positive regulation of transcription from RNA polymerase II promoter|regulation of nitrogen utilization|proteasomal ubiquitin-dependent protein catabolic process|positive regulation of endocytosis|regulation of actin cytoskeleton organization|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|regulation of protein localization|mitochondrion organization|regulation of mRNA export from nucleus|regulation of ergosterol biosynthetic process|ribophagy|Unknown|protein polyubiquitination ubiquitin binding|ubiquitin-protein ligase activity|phosphatidylinositol binding mitochondrion|ubiquitin ligase complex|Golgi apparatus|cytoplasm|cellular bud tip|endosome membrane|extrinsic to internal side of plasma membrane|plasma membrane|nucleus YKR035C_d OPI8 hom 0.07406665187273 1.78213989001818e-05 58 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Unknown Unknown Unknown YNL264C PDR17 hom FT LIPID METABOLISM 0.0740599103174309 1.75970622963211e-05 59 Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition Golgi to plasma membrane protein transport|phosphatidylserine metabolic process|response to drug|sterol biosynthetic process|Unknown|phospholipid transport|phospholipid biosynthetic process phosphatidylinositol transporter activity|phosphatidylcholine transporter activity endosome|cell periphery|cytosol|cytoplasm YDR420W HKR1 hom FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0727806558250701 2.4742565104083e-05 60 Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection (1->3)-beta-D-glucan biosynthetic process|fungal-type cell wall organization|osmosensory signaling pathway via Sho1 osmosensor|hyperosmotic response|cellular bud site selection osmosensor activity site of polarized growth|plasma membrane YNL075W IMP4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.072217807698743 2.835256433899e-05 61 Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs rRNA processing snoRNA binding|single-stranded telomeric DNA binding|rRNA primary transcript binding small-subunit processome|Mpp10 complex|90S preribosome YGR151C_d YGR151C hom 0.0721997293697612 2.87199077516778e-05 62 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Unknown Unknown Unknown YMR047C NUP116 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0721609171122929 2.87682481092966e-05 63 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) poly(A)+ mRNA export from nucleus|mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YLR164W SHH4 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0712042292421208 3.66880356418197e-05 64 Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner biological_process molecular_function mitochondrion|mitochondrial inner membrane YGL118C_d YGL118C hom 0.0705699895135349 4.30354729820722e-05 65 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR044W IOC4 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0703330405953334 4.62646664170193e-05 66 Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YEL032W MCM3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.069734962463172 5.36769875670743e-05 67 Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|double-strand break repair via break-induced replication|DNA replication initiation|pre-replicative complex assembly|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette DNA helicase activity|chromatin binding|DNA replication origin binding|ATPase activity replication fork protection complex|DNA replication preinitiation complex|pre-replicative complex|nucleus|cytoplasm|MCM complex YDR116C MRPL1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.069508738221568 5.60424874988988e-05 68 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YMR030W RSF1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0695007755943773 5.70206420634352e-05 69 Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes transcription from mitochondrial promoter|calcium-mediated signaling|transcription from RNA polymerase II promoter|aerobic respiration molecular_function mitochondrion|nucleus YKL192C ACP1 het FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0694982455975968 5.61879807198131e-05 70 Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p lipoate biosynthetic process ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process mitochondrion YJL115W ASF1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0691328351969884 6.41863514851099e-05 71 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition DNA replication-independent nucleosome assembly|histone acetylation|nucleosome disassembly|histone exchange|positive regulation of histone acetylation|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere|DNA replication-dependent nucleosome assembly|regulation of transcription from RNA polymerase II promoter in response to stress histone binding chromatin assembly complex YDR054C CDC34 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0684276378702234 7.3066925899377e-05 72 Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein autoubiquitination|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle|protein polyubiquitination ubiquitin-protein ligase activity|protein homodimerization activity SCF ubiquitin ligase complex|nucleus|cytoplasm YDR396W_d YDR396W het 0.0678765042156378 8.41395854537826e-05 73 Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Unknown Unknown Unknown YLR305C STT4 het FT LIPID METABOLISM|SIGNALING|MITOCHONDRION|PLASMA MEMBRANE 0.0677165879442676 8.6814907913146e-05 74 Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity mitochondrion|plasma membrane YBR056W YBR056W hom FT CARBOHYDRATE METABOLISM 0.0663906687830774 0.00012061525181014 75 Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function cytoplasm YMR225C MRPL44 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0663064341073424 0.00012161340132437 76 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YBR032W_d YBR032W hom 0.0662275733487609 0.00012390404901866 77 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YDR524W-C_p YDR524W-C hom 0.0661318877378279 0.000128220059579408 78 Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin biological_process molecular_function cellular_component YMR262W_p YMR262W hom 0.0659099118353164 0.000133549236075447 79 Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component YBR196C-B_p YBR196C-B hom 0.06575263324233 0.000138582350567641 80 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YBL048W_d RRT1 hom 0.0655038133560284 0.000147247025874676 81 Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YBR035C PDX3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.0649936330573848 0.000165483629718152 82 Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism fatty acid metabolic process pyridoxamine-phosphate oxidase activity cellular_component YIL046W-A_p YIL046W-A hom 0.0646592138658721 0.00017883247359702 83 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YPL003W ULA1 hom 0.0646235476572036 0.000180314383446562 84 Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation protein neddylation NEDD8 activating enzyme activity cellular_component YLR308W CDA2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0645061049229826 0.000185276036975217 85 Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall ascospore wall assembly chitin deacetylase activity chitosan layer of spore wall YOR375C GDH1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0644840742774985 0.000186220949223963 86 NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication glutamate biosynthetic process|ammonia assimilation cycle glutamate dehydrogenase (NADP+) activity nucleus|cytoplasm YOR069W VPS5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0644038995511577 0.000191807089874128 87 Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes protein retention in Golgi apparatus|retrograde transport, endosome to Golgi protein transporter activity|phosphatidylinositol-3-phosphate binding cytosol|endosome|retromer complex, outer shell|retromer complex YER101C AST2 hom FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS 0.0638009580225204 0.000217869406675809 88 Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication protein targeting to membrane molecular_function cytoplasm YLL043W FPS1 hom FT ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0636992459423557 0.000222992497476003 89 Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress conjugation with cellular fusion|transport|arsenite transport|glycerol transport|acetate transport|response to arsenic-containing substance glycerol transmembrane transporter activity|transporter activity integral to membrane|plasma membrane|fungal-type vacuole|cytoplasm YMR289W ABZ2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0634284867849302 0.000241817332255777 90 Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis folic acid biosynthetic process 4-amino-4-deoxychorismate lyase activity cytoplasm YPL152W RRD2 hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION 0.06327366098146 0.000245679542029797 91 Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress mitotic spindle organization in nucleus|response to osmotic stress peptidyl-prolyl cis-trans isomerase activity|protein phosphatase type 2A regulator activity protein phosphatase type 2A complex YKR012C_d YKR012C hom 0.0630024193900397 0.000261249497951326 92 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Unknown Unknown Unknown YMR001C-A_p YMR001C-A hom 0.0628537087094468 0.000270173654469613 93 Putative protein of unknown function biological_process molecular_function cellular_component YIL059C_d YIL059C hom 0.0624767678159142 0.000294089984635646 94 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Unknown Unknown Unknown YPR059C_d YPR059C hom 0.0622464162189953 0.000312893270471114 95 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Unknown Unknown Unknown YAL045C_d YAL045C hom 0.062066262454464 0.000322383147848407 96 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Unknown Unknown Unknown YPL165C SET6 hom 0.0619236908976464 0.000332791500321803 97 SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability biological_process molecular_function cellular_component YNL049C SFB2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0615905426707147 0.00036127545268555 98 Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat YHL039W EFM1 hom 0.0614167530539015 0.000372401073196267 99 Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity cytoplasm YKL096W-A CWP2 hom FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS 0.0613669336897961 0.000376522774515664 100 Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored fungal-type cell wall organization|regulation of pH structural constituent of cell wall fungal-type cell wall YOR193W PEX27 hom FT PEROXISOME ORGANIZATION 0.0613027490710012 0.000382665800312734 101 Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p peroxisome organization molecular_function peroxisomal membrane YEL003W GIM4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0608982541006785 0.000424980872337556 102 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YMR023C MSS1 hom FT RNA PROCESSING|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0603502919494561 0.000470530199273146 103 Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 mitochondrial tRNA wobble uridine modification|Unknown GTP binding mitochondrion|mitochondrial inner membrane YOR020C HSP10 het FT MITOCHONDRION 0.0600703724688613 0.000500019587380942 104 Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES protein folding|protein refolding unfolded protein binding|chaperone binding mitochondrion|mitochondrial matrix YOR084W LPX1 hom FT PEROXISOME ORGANIZATION 0.0600386093369151 0.000507388044547927 105 Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction peroxisome organization lipase activity peroxisomal matrix YLL032C YLL032C hom 0.0598941321803231 0.000523482159671876 106 Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene biological_process molecular_function ribosome|cytoplasm YGR079W_p YGR079W hom 0.0596534316147482 0.000547151441422223 107 Putative protein of unknown function; YGR079W is not an essential gene biological_process molecular_function cellular_component YMR168C CEP3 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0594784489840672 0.000568138079609605 108 Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain septin ring assembly|mitotic cell cycle spindle assembly checkpoint sequence-specific DNA binding|DNA binding, bending|centromeric DNA binding condensed nuclear chromosome kinetochore|CBF3 complex YLL023C POM33 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|NUCLEAR PORE 0.0594694902339801 0.000569232458132754 109 Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress nuclear pore organization|nuclear pore complex assembly structural constituent of nuclear pore ribosome|integral to membrane|endoplasmic reticulum|nuclear pore YBR173C UMP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0590261319206216 0.000625909200701324 110 Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly proteasome assembly|response to DNA damage stimulus|ubiquitin-dependent protein catabolic process molecular_function nucleus|cytoplasm YLR099C ICT1 hom FT KETONE METABOLISM|LIPID METABOLISM 0.058792131888223 0.000657897021702945 111 Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication phosphatidic acid biosynthetic process lysophosphatidic acid acyltransferase activity cellular_component YML119W_p YML119W hom 0.0587781410857482 0.000659856898893187 112 Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate biological_process molecular_function cellular_component YHR020W YHR020W het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0585312714948722 0.000701793349656216 113 Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments chromosome organization|tRNA aminoacylation for protein translation proline-tRNA ligase activity ribosome YLR037C PAU23 hom FT CELL WALL ORG/BIOGENESIS 0.0581020265686386 0.000768263641367997 114 Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function fungal-type cell wall YBR200W BEM1 hom FT SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0580889951868127 0.000763390484235641 115 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p establishment of cell polarity|cell morphogenesis involved in conjugation with cellular fusion protein complex scaffold|phosphatidylinositol-3-phosphate binding incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip YNL101W AVT4 hom FT VACUOLAR TRANSPORT 0.0579665793474638 0.000783288464561711 116 Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters amino acid export from vacuole transporter activity|L-glutamine transmembrane transporter activity|L-isoleucine transmembrane transporter activity|L-tyrosine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YIL023C YKE4 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0576729791816606 0.000832988910911112 117 Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family zinc ion transport zinc ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YNL268W LYP1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0572698912402804 0.000905995519949888 118 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids basic amino acid transport basic amino acid transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YLR133W CKI1 hom FT LIPID METABOLISM 0.0571402645064588 0.000930704817756989 119 Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolamine kinase activity|choline kinase activity cytoplasm YOR347C PYK2 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0570880644150335 0.000940830253899638 120 Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication pyruvate metabolic process pyruvate kinase activity cytosol|mitochondrion YLR335W NUP2 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CHROMOSOME|NUCLEAR PORE 0.0566830162914856 0.00102471476172468 121 Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization rRNA export from nucleus|snRNA export from nucleus|nuclear pore organization|tRNA export from nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|mRNA-binding (hnRNP) protein import into nucleus|ribosomal protein import into nucleus|snRNP protein import into nucleus|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette|protein targeting to membrane|poly(A)+ mRNA export from nucleus|protein export from nucleus|mRNA export from nucleus in response to heat stress|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery|NLS-bearing substrate import into nucleus|mRNA export from nucleus|nuclear-transcribed mRNA catabolic process, non-stop decay nucleocytoplasmic transporter activity nuclear chromatin|mitochondrion|integral to membrane|nuclear pore YHL035C VMR1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0566496199710413 0.00102999191683764 122 Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions transport|response to drug|response to metal ion|drug transport ATPase activity, coupled to transmembrane movement of substances fungal-type vacuole membrane|mitochondrion|integral to membrane|ribosome YGR156W PTI1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0565890208430769 0.00104291252516382 123 Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 termination of RNA polymerase II transcription, exosome-dependent|mRNA cleavage|termination of RNA polymerase II transcription, poly(A)-coupled|mRNA polyadenylation|snoRNA 3'-end processing RNA binding mRNA cleavage and polyadenylation specificity factor complex|nucleus YER065C ICL1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.0565759082521641 0.00104572797488435 124 Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose glyoxylate cycle isocitrate lyase activity cellular_component YML018C YML018C hom 0.0564489160849207 0.00108265989065441 125 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication biological_process molecular_function fungal-type vacuole membrane|integral to membrane YIL065C FIS1 hom FT MITOCHONDRION ORGANIZATION|PEROXISOME ORGANIZATION|MITOCHONDRION 0.056148361148106 0.00114147185020109 126 Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation peroxisome fission|mitochondrial fission|chronological cell aging|peroxisome organization molecular_function mitochondrial outer membrane|mitochondrion|peroxisome YGL075C MPS2 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0560413128636118 0.00116668005145944 127 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p mitotic anaphase|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton nuclear envelope|spindle pole body YPR168W NUT2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0556489949933905 0.00126352518840359 128 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YDR020C_p DAS2 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0555525913021511 0.00128843223347814 129 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases biological_process molecular_function nucleus|cytoplasm YHR007C ERG11 het FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0554012764374677 0.00132844116644688 130 Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p ergosterol biosynthetic process sterol 14-demethylase activity endoplasmic reticulum YLR097C HRT3 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.0553278577336393 0.00134826375130309 131 Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YMR001C CDC5 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0551455443761283 0.00141493683967294 132 Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate synaptonemal complex disassembly|spindle assembly involved in meiosis|protein phosphorylation|resolution of meiotic recombination intermediates|positive regulation of spindle pole body separation protein kinase activity nucleus|cellular bud neck|spindle pole YOR121C_d YOR121C hom 0.0549802724107228 0.00145778690957886 133 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Unknown Unknown Unknown YDR275W BSC2 hom 0.0548572007920057 0.00148195539568742 134 Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function lipid particle YDR415C_p YDR415C hom FT PROTEOLYSIS 0.0548285683311919 0.00149046991533219 135 Putative aminopeptidase biological_process molecular_function cellular_component YLR323C CWC24 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0546892870185318 0.00153253468756425 136 Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p generation of catalytic spliceosome for first transesterification step|snoRNA splicing molecular_function nucleus|U2-type spliceosomal complex YHR045W_p YHR045W hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.0544493932136939 0.00160755668534259 137 Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum biological_process molecular_function endoplasmic reticulum YOR269W PAC1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CYTOSKELETON 0.0543876892097424 0.00162739226257615 138 Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly nuclear migration along microtubule microtubule plus-end binding cytoplasmic microtubule|nucleus|cytoplasm YLR331C_d JIP3 hom 0.054318281294488 0.0016526246607152 139 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Unknown Unknown Unknown YBR170C NPL4 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0539922155967818 0.0017599620646079 140 Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) retrograde protein transport, ER to cytosol|proteasomal ubiquitin-dependent protein catabolic process|mitochondria-associated protein catabolic process|mitotic spindle disassembly|ER-associated protein catabolic process|ER-associated misfolded protein catabolic process|Unknown|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process molecular_function Doa10p ubiquitin ligase complex|integral to membrane|Cdc48p-Npl4p-Ufd1p AAA ATPase complex|Cdc48p-Npl4p-Vms1p AAA ATPase complex|nuclear outer membrane-endoplasmic reticulum membrane network YDR060W MAK21 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0538163212010772 0.00182205485191434 141 Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein ribosomal large subunit assembly molecular_function Noc1p-Noc2p complex YHR037W PUT2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.053782574117557 0.00185162616325933 142 Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism glutamate biosynthetic process|proline catabolic process to glutamate 1-pyrroline-5-carboxylate dehydrogenase activity mitochondrion|mitochondrial matrix YPL175W SPT14 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0535798014521851 0.00194491732302649 143 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|endoplasmic reticulum YOL136C PFK27 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0535414594247371 0.00192613198755275 144 6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YGL090W LIF1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0530785483087833 0.00210503623613267 145 Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein double-strand break repair via nonhomologous end joining chromatin binding DNA ligase IV complex|nuclear chromatin|nucleus YDR351W SBE2 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|GOLGI APPARATUS 0.0530123918266263 0.00213228541057308 146 Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth fungal-type cell wall organization molecular_function Golgi apparatus YNL151C RPC31 het FT NUCLEUS 0.0528476229935364 0.0022218110694482 147 RNA polymerase III subunit C31 transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YOR029W_d YOR029W hom 0.0526570252678127 0.00228426959023006 148 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR204C LDH1 hom FT LIPID METABOLISM 0.0524848526448829 0.00236141638155261 149 Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) lipid homeostasis hydrolase activity, acting on ester bonds|serine hydrolase activity|triglyceride lipase activity lipid particle YHR159W_p TDA11 hom 0.0522260716226082 0.0024818636559196 150 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function cytoplasm YMR284W YKU70 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME 0.0518180850126388 0.00269507718723951 151 Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair chromatin assembly or disassembly|chromatin silencing|double-strand break repair via nonhomologous end joining|telomere maintenance|double-strand break repair via break-induced replication|double-strand break repair via homologous recombination|chromatin silencing at silent mating-type cassette damaged DNA binding|RNA binding nuclear chromatin|nuclear telomeric heterochromatin|nuclear envelope|Ku70:Ku80 complex YER001W MNN1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0518097168932497 0.00268749054659261 152 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family N-glycan processing|protein O-linked glycosylation alpha-1,3-mannosyltransferase activity Golgi apparatus YGL145W TIP20 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0517434146708279 0.00272163593600731 153 Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p retrograde vesicle-mediated transport, Golgi to ER molecular_function Dsl1p complex|endoplasmic reticulum YMR134W ERG29 het FT LIPID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0516645019813176 0.00277900807829954 154 Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes cellular iron ion homeostasis|mitochondrion organization molecular_function nuclear envelope|endoplasmic reticulum|cytoplasm YBL012C_d YBL012C hom 0.0516079358821502 0.00279264192568477 155 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL106W MOB1 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.0515230014156731 0.00283801530160982 156 Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress regulation of exit from mitosis|protein phosphorylation kinase regulator activity spindle pole body|cellular bud neck YDR055W PST1 hom FT CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0514428584370223 0.00292357682600945 157 Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 fungal-type cell wall organization molecular_function fungal-type cell wall|plasma membrane YGR228W_d YGR228W hom 0.0511603164634495 0.00303943623548704 158 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Unknown Unknown Unknown YPL041C_p YPL041C hom 0.0510801167652809 0.00311680079065085 159 Protein of unknown function involved in maintenance of proper telomere length biological_process molecular_function cellular_component YOL128C YGK3 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION 0.0510483967091981 0.00313542512377622 160 Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation proteolysis|protein phosphorylation|response to stress protein serine/threonine kinase activity|protein kinase activity cellular_component YBL009W ALK2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION 0.0507768342390945 0.00326650955819753 161 Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins meiosis|mitosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity cellular_component YDR496C PUF6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|SITE OF POLARIZED GROWTH|RIBOSOME 0.0507643270320728 0.00329743766102453 162 Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis ribosomal large subunit biogenesis|negative regulation of translation mRNA 3'-UTR binding|translation repressor activity, nucleic acid binding nucleolus|nucleus|large ribosomal subunit YML074C FPR3 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0507286633406596 0.00330076520666 163 Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p meiotic recombination checkpoint peptidyl-prolyl cis-trans isomerase activity|macrolide binding nucleolus YNL247W YNL247W het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.050537654056659 0.00341585050838128 164 Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments cysteinyl-tRNA aminoacylation cysteine-tRNA ligase activity ribosome|cytoplasm YHL015W-A_p YHL015W-A hom 0.0504761627582513 0.00347953347875216 165 Putative protein of unknown function biological_process molecular_function cellular_component YDR211W GCD6 het FT TRANSLATION 0.0503825984335169 0.00351595703278577 166 Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress regulation of translational initiation guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YKL120W OAC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0503464530805435 0.0035446138815018 167 Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family sulfate transport|oxaloacetate transport|isopropylmalate transport secondary active sulfate transmembrane transporter activity|oxaloacetate secondary active transmembrane transporter activity|isopropylmalate transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YNL104C LEU4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0501953677148302 0.00364038894077948 168 Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication leucine biosynthetic process 2-isopropylmalate synthase activity mitochondrion|cytoplasm YMR164C MSS11 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0501451091004129 0.00367446504789415 169 Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YNL058C_p YNL058C hom 0.0500376870535306 0.0037482727403173 170 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene biological_process molecular_function fungal-type vacuole YOL135C MED7 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0500000203470363 0.00377447010253688 171 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YER145C FTR1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0499994474045908 0.00377486986764127 172 High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress iron assimilation by reduction and transport|high-affinity iron ion transport|transmembrane transport iron ion transmembrane transporter activity high affinity iron permease complex|integral to membrane|plasma membrane YPR165W RHO1 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|CYTOSKELETON ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0497898419855845 0.00392372770165377 173 GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) regulation of fungal-type cell wall (1->3)-alpha-glucan biosynthetic process|regulation of fungal-type cell wall organization|positive regulation of endocytosis|budding cell bud growth|small GTPase mediated signal transduction|regulation of protein localization|positive regulation of protein kinase C signaling cascade|actin cytoskeleton organization|regulation of vacuole fusion, non-autophagic|actin cytoskeleton reorganization|regulation of exocyst localization|regulation of cell size GTPase activity|signal transducer activity incipient cellular bud site|mitochondrion|Golgi apparatus|peroxisome|cellular bud neck|cellular bud tip|mitochondrial outer membrane|1,3-beta-D-glucan synthase complex|plasma membrane|mating projection tip YGL023C PIB2 hom FT VESICLE-MEDIATED TRANSPORT|MITOCHONDRION 0.0496048273146478 0.00408717004395815 174 Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 biological_process molecular_function mitochondrion YGL200C EMP24 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0493713287399162 0.00426559736302081 175 Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles protein retention in ER lumen|vesicle organization|ER to Golgi vesicle-mediated transport molecular_function integral to membrane|endoplasmic reticulum|ER to Golgi transport vesicle YOR126C IAH1 hom FT KETONE METABOLISM|LIPID METABOLISM 0.0492273015187766 0.00437914671202932 176 Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing acetate metabolic process hydrolase activity, acting on ester bonds cellular_component YER157W COG3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0491569348217292 0.00440610943867945 177 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|ER to Golgi vesicle-mediated transport|macroautophagy|retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway protein transporter activity Golgi transport complex YDL203C ACK1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0490926804352701 0.00445797063595212 178 Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria fungal-type cell wall organization|positive regulation of signal transduction molecular_function mitochondrion YBR247C ENP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0490222100492953 0.00451548410218984 179 Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing snoRNA binding preribosome, small subunit precursor|nucleolus|nucleus|cytoplasm|90S preribosome YOR060C SLD7 het FT CELL CYCLE|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CYTOSKELETON 0.0487453207977065 0.00474802717732877 180 Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria regulation of DNA-dependent DNA replication initiation molecular_function nuclear envelope|endoplasmic reticulum|DNA replication preinitiation complex|nucleus|spindle pole body|cytoplasm YOR015W_d YOR015W hom 0.0487172191569086 0.00477222359608962 181 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL116C PRR1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN PHOSPHORYLATION 0.048601075898857 0.00487341427494299 182 Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor negative regulation of transcription from RNA polymerase II promoter by pheromones|negative regulation of conjugation with cellular fusion|protein phosphorylation protein kinase activity|receptor signaling protein serine/threonine kinase activity cytoplasm YDR253C MET32 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|NUCLEUS 0.048548975982526 0.00493872405994192 183 Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication regulation of mitotic cell cycle|regulation of sulfur amino acid metabolic process|regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|RNA polymerase II activating transcription factor binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YJR103W URA8 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0485029501429095 0.00496041480378575 184 Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YNR056C BIO5 hom FT KETONE METABOLISM|COFACTOR METABOLISM|PLASMA MEMBRANE 0.0483260925291441 0.0051274016489488 185 Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis biotin biosynthetic process|vitamin transport transmembrane transporter activity integral to membrane|plasma membrane YHR021C RPS27B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0482931339038701 0.00515117017120074 186 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YFR016C YFR016C hom 0.0481157772357366 0.0053175556850227 187 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene biological_process molecular_function cellular bud|cytoplasm YGR074W SMD1 het FT RNA PROCESSING|NUCLEUS 0.0479571652114389 0.00547044926958786 188 Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U4/U6 x U5 tri-snRNP complex|U2-type prespliceosome|U5 snRNP YDL237W AIM6 hom 0.0478543126119139 0.00557170220176332 189 Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene biological_process molecular_function cellular_component YML104C MDM1 hom FT MITOCHONDRION ORGANIZATION|CYTOSKELETON 0.0478384891145645 0.00560875223201695 190 Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) nuclear migration|mitochondrion inheritance|mitochondrion organization structural constituent of cytoskeleton|phosphatidylinositol-3-phosphate binding intermediate filament|integral to membrane|cytoplasm YCR084C TUP1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0473842329632091 0.00605632068418746 191 General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes nucleosome positioning|regulation of transcription from RNA polymerase II promoter in response to osmotic stress|histone exchange|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by glucose|negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to hypoxia|negative regulation of transcription from RNA polymerase II promoter during mitosis|Unknown ubiquitin binding|histone deacetylase binding|histone binding|phosphatidylinositol-3,5-bisphosphate binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription|mediator complex binding nucleus|transcriptional repressor complex YDL062W_d YDL062W hom 0.0472466250219165 0.00620519559190474 192 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Unknown Unknown Unknown YJL083W TAX4 hom FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS 0.0471850762192345 0.0062728435307643 193 EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication autophagy|fungal-type cell wall organization|inositol lipid-mediated signaling|CVT pathway|cellular response to starvation molecular_function pre-autophagosomal structure YNL086W_p SNN1 hom 0.0471622026046134 0.00630594786558894 194 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes biological_process molecular_function endosome|BLOC-1 complex YKL205W LOS1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0470439750217407 0.00645423570558636 195 Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm tRNA re-export from nucleus|tRNA splicing, via endonucleolytic cleavage and ligation|tRNA export from nucleus tRNA binding|Ran GTPase binding mitochondrion|integral to membrane|nuclear matrix|cytoplasm YOR268C_p YOR268C hom 0.0470434019746679 0.00643108501351396 196 Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene biological_process molecular_function cellular_component YLR390W-A CCW14 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0469484898498993 0.00657126172813738 197 Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall fungal-type cell wall organization structural constituent of cell wall mitochondrion|fungal-type cell wall YBL029W_p YBL029W hom FT NUCLEUS 0.0469483866746462 0.00653921308847559 198 Non-essential protein of unknown function biological_process molecular_function nucleus|cytoplasm YPL089C RLM1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|NUCLEUS 0.0469082533452277 0.00658537400410532 199 MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication signal transduction|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|DNA binding, bending|sequence-specific DNA binding nucleus YJL104W PAM16 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0468899031182984 0.00660657764514698 200 Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain protein import into mitochondrial matrix protein domain specific binding mitochondrion|presequence translocase-associated import motor|mitochondrial inner membrane presequence translocase complex YCR043C_p YCR043C hom FT GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.046889382124586 0.00660718054687435 201 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene biological_process molecular_function Golgi apparatus|mating projection tip YPR200C ARR2 hom 0.0467771952802066 0.00674637832544654 202 Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p response to arsenic-containing substance arsenate reductase activity cellular_component YBR201W DER1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0465073449179467 0.00706282550505809 203 Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p ER-associated protein catabolic process molecular_function integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YDR316W OMS1 hom FT MITOCHONDRION 0.0464417508147001 0.00715243125604653 204 Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations biological_process S-adenosylmethionine-dependent methyltransferase activity mitochondrial envelope|mitochondrion YDR409W SIZ1 hom FT CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.0463729655588744 0.00722967953849587 205 SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring protein sumoylation|chromosome segregation SUMO ligase activity nuclear chromatin|septin ring YBR156C SLI15 hom FT CELL DIVISION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0462832758166624 0.00741355425139096 206 Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly chromosome segregation|protein phosphorylation|regulation of cytokinesis protein kinase activator activity spindle microtubule|spindle midzone|chromosome passenger complex|kinetochore microtubule YOR006C TSR3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0460458796880702 0.00765068075526988 207 Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress maturation of SSU-rRNA molecular_function nucleus|cytoplasm YOR176W HEM15 het FT COFACTOR METABOLISM|MITOCHONDRION 0.0460163156697459 0.00769889445322796 208 Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway heme biosynthetic process ferrochelatase activity mitochondrion|mitochondrial inner membrane YIL019W FAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0458629848133409 0.00790483216836027 209 Protein required for pre-rRNA processing and 40S ribosomal subunit assembly maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|cytoplasm YDR436W PPZ2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0458582018964252 0.00790204273048213 210 Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular sodium ion homeostasis protein serine/threonine phosphatase activity cellular_component YKL044W_p YKL044W hom 0.0456983938955581 0.0081218791361903 211 Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YNL041C COG6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0456557445782652 0.00821013002105391 212 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YLL029W FRA1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS 0.0453716502599606 0.00861821305501536 213 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation negative regulation of transcription from RNA polymerase II promoter in response to iron molecular_function cytosol|cytoplasm YPL119C DBP1 hom FT TRANSLATION 0.0447181675550322 0.00959407679566731 214 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress translational initiation RNA helicase activity cytoplasm YMR171C EAR1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0446426304837013 0.00971683418361478 215 Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function endosome YDR385W EFT2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0445714704347531 0.00983375642237487 216 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication translational elongation|positive regulation of translational elongation translation elongation factor activity ribosome YPR159W KRE6 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0445576865671292 0.00987855863637897 217 Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p fungal-type cell wall organization|(1->6)-beta-D-glucan biosynthetic process glucosidase activity integral to endoplasmic reticulum membrane|integral to membrane|site of polarized growth|transport vesicle|plasma membrane YER183C FAU1 hom FT COFACTOR METABOLISM|MITOCHONDRION 0.0445319066849402 0.00991033843504381 218 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis folic acid-containing compound biosynthetic process 5-formyltetrahydrofolate cyclo-ligase activity mitochondrion YER097W_d YER097W hom 0.0444325788530383 0.0100655829758396 219 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR181C_p YMR181C hom 0.0444083185973983 0.0101065716142666 220 Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication biological_process molecular_function cellular_component YLR089C ALT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0443904960336487 0.0101367781141369 221 Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive alanine catabolic process|alanine biosynthetic process L-alanine:2-oxoglutarate aminotransferase activity mitochondrion YLR051C FCF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0443363396915751 0.0102403698407657 222 Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YPR106W ISR1 hom FT PROTEIN PHOSPHORYLATION 0.0441552786349063 0.0105430300325645 223 Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C biological_process protein kinase activity cellular_component YDR295C HDA2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0439939208353956 0.0108300250471225 224 Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance negative regulation of transcription from RNA polymerase II promoter|gene silencing by RNA|histone deacetylation|gene silencing involved in chronological cell aging histone deacetylase activity|chromatin binding|DNA binding nucleus|HDA1 complex YMR166C_p YMR166C hom FT MITOCHONDRION 0.0438213503797083 0.0111929669522399 225 Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene transport transporter activity integral to membrane|mitochondrial inner membrane YDR021W FAL1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.043781638812579 0.0112181585792956 226 Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus|FAl1-SGD1 complex YLR047C FRE8 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0437780032434798 0.0112249119958858 227 Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p cellular metal ion homeostasis oxidoreductase activity, oxidizing metal ions integral to membrane|membrane YPR020W ATP20 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0437548204261451 0.0112680612333956 228 Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex protein oligomerization|cristae formation|protein complex assembly|ATP synthesis coupled proton transport structural molecule activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YNL272C SEC2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SITE OF POLARIZED GROWTH 0.0437483387747751 0.0112801515733795 229 Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles autophagy|exocytosis guanyl-nucleotide exchange factor activity|phosphatidylinositol-4-phosphate binding cytosol|transport vesicle|mating projection tip|cellular bud neck|cellular bud tip YGL222C EDC1 hom FT RNA PROCESSING|TRANSLATION|NUCLEUS 0.0437392926616172 0.0112970446976733 230 RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|positive regulation of translation in response to stress mRNA binding nucleus|cytoplasm YDL234C GYP7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM 0.0436876227097635 0.0113939666052273 231 GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking regulation of vacuole fusion, non-autophagic|vesicle-mediated transport Rab GTPase activator activity cytoplasm YOR064C YNG1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0435787267243669 0.0116629553492059 232 Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 histone acetylation|chromatin modification histone acetyltransferase activity|methylated histone residue binding NuA3 histone acetyltransferase complex YDL223C HBT1 hom 0.0435105978955393 0.0118449850528065 233 Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis cell morphogenesis involved in conjugation with cellular fusion molecular_function plasma membrane|mating projection YGR234W YHB1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0433414980533973 0.0121137513132825 234 Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress response to stress nitric oxide reductase activity cytosol|mitochondrion|mitochondrial matrix|cytoplasm YIL130W ASG1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0433395513267101 0.012066319409483 235 Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance biological_process sequence-specific DNA binding nucleus YJL163C_p YJL163C hom 0.0432534129078391 0.0122509805760579 236 Putative protein of unknown function biological_process molecular_function integral to membrane YOR252W TMA16 hom FT NUCLEUS 0.0430838198107675 0.0125824704871564 237 Protein of unknown function that associates with ribosomes biological_process molecular_function ribosome|nucleus YHR015W MIP6 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0429713773618018 0.0128155349382234 238 Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication mRNA export from nucleus RNA binding nuclear pore YDR476C_p YDR476C hom FT ENDOPLASMIC RETICULUM 0.0429291338758642 0.0129444981751691 239 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene biological_process molecular_function endoplasmic reticulum YPL073C_d YPL073C hom 0.0428265448113089 0.0131213578950573 240 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Unknown Unknown Unknown YBR299W MAL32 hom FT CARBOHYDRATE METABOLISM 0.04270314437843 0.0133869967811656 241 Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YOR133W EFT1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0426295109701491 0.013547757692836 242 Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication translational elongation translation elongation factor activity ribosome YPL091W GLR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0425042597416656 0.0138251328624809 243 Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|protein glutathionylation glutathione-disulfide reductase activity mitochondrion|nucleus|cytoplasm YPL141C FRK1 hom FT PROTEIN PHOSPHORYLATION 0.0423018276174305 0.0143276019638375 244 Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication protein phosphorylation protein kinase activity cytoplasm YER134C YER134C hom FT NUCLEUS 0.0421512453022315 0.0146340530588844 245 Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene biological_process acid phosphatase activity nucleus|cytoplasm YHR178W STB5 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0421175529218059 0.01507351607715 246 Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro response to xenobiotic stimulus|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YOR342C YOR342C hom FT NUCLEUS 0.0419872526745604 0.0150238214100852 247 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YLL019C KNS1 hom FT PROTEIN PHOSPHORYLATION 0.041966303229868 0.0150742655120019 248 Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation negative regulation of transcription from RNA polymerase III promoter|protein autophosphorylation|protein phosphorylation protein serine/threonine kinase activity|protein tyrosine kinase activity|protein kinase activity nucleus|cytoplasm YER087C-A_d YER087C-A hom 0.0419379375045467 0.0151428048599724 249 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Unknown Unknown Unknown YOR329C SCD5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0418248214096099 0.0154188599114827 250 Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency protein secretion|endocytosis|actin filament organization|cortical actin cytoskeleton organization protein phosphatase 1 binding nucleus|actin cortical patch YPR130C_d YPR130C hom 0.0416496896682262 0.0158549974617538 251 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR319C_p YFT2 hom 0.0414381298013205 0.0163962869301047 252 Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function integral to membrane YMR294W JNM1 hom FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0411494997005224 0.0171609063563445 253 Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B nuclear migration|establishment of mitotic spindle orientation structural constituent of cytoskeleton dynactin complex|cell cortex|spindle pole body|astral microtubule YKR078W YKR078W hom 0.0411143655579216 0.0175414092293285 254 Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) biological_process phosphatidylinositol-3-phosphate binding cytoplasm YKL148C SDH1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0410367516099243 0.0174679787258326 255 Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits cellular respiration|mitochondrial electron transport, succinate to ubiquinone|tricarboxylic acid cycle succinate dehydrogenase (ubiquinone) activity|flavin adenine dinucleotide binding mitochondrial respiratory chain complex II|mitochondrion|mitochondrial membrane YJR065C ARP3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0410248051121827 0.0175007957758098 256 Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity mitochondrion inheritance|Arp2/3 complex-mediated actin nucleation|actin filament organization ATP binding Arp2/3 protein complex|plasma membrane YDR525W-A SNA2 hom 0.0408313508189641 0.0180397862612186 257 Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function fungal-type vacuole membrane|integral to membrane|cytoplasm YJR008W MHO1 hom FT NUCLEUS 0.0408017876908657 0.0182098374596865 258 Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility biological_process molecular_function nucleus|cytoplasm YNR061C YNR061C hom 0.0407209239746213 0.0183539210207122 259 Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress biological_process molecular_function integral to membrane|fungal-type vacuole YLR207W HRD3 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.040649231592519 0.0185604197534017 260 Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events ER-associated protein catabolic process ubiquitin-protein ligase activity Hrd1p ubiquitin ligase ERAD-M complex|luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YCR075W-A_p YCR075W-A hom 0.0405936249931625 0.0187219824092357 261 Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YKL048C ELM1 hom FT CELL CYCLE|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.040447182284457 0.0192252531379023 262 Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring positive regulation of protein autophosphorylation|protein autophosphorylation|cell morphogenesis|budding cell bud growth|protein phosphorylation|response to drug|septin checkpoint|pseudohyphal growth|glucose metabolic process|axial cellular bud site selection|response to osmotic stress|cytokinesis protein serine/threonine kinase activity|protein kinase activity cellular bud neck contractile ring YIL038C NOT3 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0403604664938226 0.0194128768413983 263 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|positive regulation of transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|cytoplasm YER055C HIS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0403112802461843 0.0195614370835689 264 ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control histidine biosynthetic process ATP phosphoribosyltransferase activity cellular_component YGR181W TIM13 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0402053012904628 0.019884909161653 265 Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity mitochondrion|integral to membrane|mitochondrial intermembrane space protein transporter complex|mitochondrial intermembrane space YKR009C FOX2 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0401061755261247 0.0202103131686313 266 Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities fatty acid beta-oxidation enoyl-CoA hydratase activity|3-hydroxyacyl-CoA dehydrogenase activity peroxisome YHL009C YAP3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0400946088966264 0.0202277418880051 267 Basic leucine zipper (bZIP) transcription factor regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YBR004C GPI18 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0400759241898874 0.0202861172153924 268 Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria GPI anchor biosynthetic process mannosyltransferase activity|dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to endoplasmic reticulum membrane|mitochondrion|mannosyltransferase complex|integral to membrane|endoplasmic reticulum YPL060W MFM1 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.039828156731102 0.0210742014707234 269 Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity integral to membrane|mitochondrial inner membrane YJL151C SNA3 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.0398164665299309 0.0211120347959102 270 Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles cellular protein catabolic process|endosome transport via multivesicular body sorting pathway ubiquitin protein ligase binding fungal-type vacuole lumen|integral to membrane YCL042W_p YCL042W hom 0.0397406281284381 0.0213977136597361 271 Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm YGR039W_d YGR039W hom 0.0397145160896858 0.0214444946256882 272 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Unknown Unknown Unknown YGR065C VHT1 het FT PLASMA MEMBRANE 0.0395529549436252 0.0220599404660597 273 High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin biotin transport biotin transporter activity integral to membrane|plasma membrane YNR025C_d YNR025C hom 0.0394692296307834 0.0222631006297509 274 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Unknown Unknown Unknown YKL025C PAN3 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION 0.0394632524810437 0.0222833828912803 275 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes DNA repair|mRNA 3'-end processing|postreplication repair poly(A)-specific ribonuclease activity PAN complex|cytoplasm YIL076W SEC28 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0394161787364273 0.0224436802887081 276 Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport|vesicle coating molecular_function endosome|COPI vesicle coat YJL025W RRN7 het FT NUCLEUS 0.0393758692899345 0.0226625185301063 277 Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I CORE element sequence-specific DNA binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity nucleolus|RNA polymerase I core factor complex YMR272C SCS7 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0393335535240378 0.0228289402986954 278 Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth inositolphosphoceramide metabolic process fatty acid alpha-hydroxylase activity integral to membrane|endoplasmic reticulum|membrane YDR254W CHL4 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0393279872648789 0.0227466918587711 279 Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 kinetochore assembly|chromosome segregation|establishment of meiotic sister chromatid cohesion|protein localization to chromosome, centromeric region|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion structural molecule activity condensed nuclear chromosome outer kinetochore YGL060W YBP2 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0393265468548409 0.0227516702203986 280 Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication G2/M transition of mitotic cell cycle molecular_function condensed nuclear chromosome kinetochore|centromere-specific nucleosome|cytoplasm YJL029C VPS53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0392497437521905 0.0230184920317308 281 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting Golgi to vacuole transport|retrograde transport, endosome to Golgi molecular_function Golgi apparatus|GARP complex|cytoplasm YKL053C-A MDM35 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0390701753700977 0.0236529537682565 282 Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress mitochondrion organization|mitochondrial respiratory chain complex assembly molecular_function nucleus|cytoplasm|mitochondrial intermembrane space YHR191C CTF8 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0390085391404044 0.0239371598793838 283 Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion maintenance of DNA trinucleotide repeats|mitotic sister chromatid cohesion molecular_function Ctf18 RFC-like complex YGR090W UTP22 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0388769819351453 0.0244162747834891 284 Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|UTP-C complex|90S preribosome|CURI complex YFL001W DEG1 hom FT RNA PROCESSING|NUCLEUS 0.0388553849597066 0.0246241988841935 285 tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity nucleus|cytoplasm YBL091C-A SCS22 hom FT LIPID METABOLISM 0.038845629423304 0.0244676297297729 286 Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication phospholipid biosynthetic process|chromatin silencing at telomere molecular_function cellular_component YCL004W PGS1 het FT LIPID METABOLISM|MITOCHONDRION 0.0388295462720784 0.0245269028116731 287 Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis cardiolipin biosynthetic process CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity mitochondrion YHR189W PTH1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0388179943923662 0.0246767005272838 288 One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium mitochondrial translation aminoacyl-tRNA hydrolase activity mitochondrion YBR001C NTH2 hom FF|FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0387973929450777 0.0246457740778603 289 Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication trehalose catabolic process alpha,alpha-trehalase activity mitochondrion|cytoplasm YDL120W YFH1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0387897390700726 0.0246741439542531 290 Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia cellular iron ion homeostasis|iron-sulfur cluster assembly|glutathione metabolic process ferrous iron binding|ferroxidase activity|iron chaperone activity mitochondrion|mitochondrial matrix YPL157W TGS1 hom FT CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0387413812112452 0.0248540418846619 291 Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs meiosis|RNA methylation|nucleologenesis|7-methylguanosine cap hypermethylation|regulation of telomere maintenance via telomerase RNA methyltransferase activity nucleolus YIR005W IST3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.0387039905656877 0.0249939175669606 292 Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing mRNA export from nucleus|spliceosomal complex assembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step first spliceosomal transesterification activity RES complex|U2 snRNP YBR130C SHE3 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.038652361789918 0.0251881766150621 293 Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance mating type switching|endoplasmic reticulum inheritance|intracellular mRNA localization mRNA binding actin cap|cytoplasm|cellular bud tip YLR409C UTP21 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0386039203354702 0.0253716294835497 294 Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma ribosomal small subunit biogenesis|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YNL207W RIO2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0385023276154108 0.025760128534476 295 Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) nucleocytoplasmic transporter activity|protein kinase activity cytosol|nucleus|cytoplasm YNL226W_d YNL226W hom 0.0383922480159705 0.026186876712299 296 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Unknown Unknown Unknown YDR113C PDS1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0383676655279259 0.026372774560758 297 Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation protein localization|recombinational repair|meiosis I|mitotic sister chromatid segregation enzyme binding nucleus|cytoplasm|spindle YHL041W_d YHL041W hom 0.0383372490418444 0.0264023711410615 298 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YAR015W ADE1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.038311458435003 0.0265264909165203 299 N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolesuccinocarboxamide synthase activity nucleus|cytoplasm YNL339C YRF1-6 hom FT NUCLEUS 0.0381675521655811 0.027099825018812 300 Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p telomere maintenance via recombination DNA helicase activity nucleus YLR340W RPP0 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0379423044280517 0.0283484951577387 301 Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 translational elongation|cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|LSU rRNA binding cytosolic large ribosomal subunit|preribosome, large subunit precursor|90S preribosome|cytoplasm YOL064C MET22 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0378708951400779 0.0282919682877671 302 Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway methionine biosynthetic process|hyperosmotic salinity response|sulfate assimilation 3'(2'),5'-bisphosphate nucleotidase activity cellular_component YKL046C DCW1 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0378107365129291 0.0285440062991914 303 Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p fungal-type cell wall biogenesis|budding cell bud growth mannosidase activity anchored to plasma membrane YLR446W_p YLR446W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0377955972763746 0.0286314890281442 304 Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene biological_process molecular_function cellular_component YNL310C ZIM17 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0377922948348806 0.0286216548607981 305 Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix protein folding|protein stabilization|protein import into mitochondrial matrix|response to unfolded protein|mitochondrion organization chaperone binding mitochondrion|mitochondrial matrix YDR167W TAF10 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.0376413956894981 0.0292638735344803 306 Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|protein complex scaffold SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YMR286W MRPL33 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0376393014175262 0.0294179445805425 307 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLL060C GTT2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0376365212313018 0.029284823962025 308 Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress glutathione metabolic process glutathione transferase activity mitochondrion YMR089C YTA12 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.037573942399138 0.0295549357591596 309 Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes proteolysis|signal peptide processing|protein complex assembly|protein import into mitochondrial intermembrane space metallopeptidase activity|protein binding|ATPase activity|ATP binding mitochondrion|mitochondrial inner boundary membrane|mitochondrial inner membrane|m-AAA complex|cytoplasm YBR025C OLA1 hom 0.0375467849246015 0.0296728215766641 310 P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress biological_process ATPase activity cytoplasm YJR135C MCM22 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.037539582313188 0.029704154573919 311 Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YPR113W PIS1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0375266968533148 0.0298335383948825 312 Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins phosphatidylinositol biosynthetic process CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity mitochondrial outer membrane|mitochondrion|integral to membrane|Golgi apparatus YNR040W_p YNR040W hom FT MITOCHONDRION 0.0375125416404101 0.0298464744179193 313 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YBR203W COS111 hom FT SIGNALING|MITOCHONDRION 0.0374975527084635 0.0299610308477404 314 Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signal transduction molecular_function mitochondrion YMR092C AIP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0374666535856876 0.0300230161038376 315 Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress actin filament depolymerization|response to osmotic stress actin binding nucleus|mating projection tip|cytoplasm|actin cortical patch YDR052C DBF4 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION 0.0374309540185284 0.0301801721438086 316 Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress negative regulation of exit from mitosis|DNA replication initiation|positive regulation of protein kinase activity DNA replication origin binding|protein serine/threonine kinase activator activity Dbf4-dependent protein kinase complex YPL054W LEE1 hom 0.037386907157854 0.0303750477896697 317 Zinc-finger protein of unknown function biological_process molecular_function cellular_component YKL214C YRA2 hom FT RNA LOCALIZATION|NUCLEUS 0.0371828931699357 0.0312918170390723 318 Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus poly(A)+ mRNA export from nucleus RNA binding nucleus YLR053C_p YLR053C hom 0.0371420196007719 0.0317326804760967 319 Putative protein of unknown function biological_process molecular_function cellular_component YPR143W RRP15 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0371095823686434 0.0316269971200565 320 Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|preribosome, large subunit precursor YPR183W DPM1 het FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0370866371745375 0.0317580117621567 321 Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation GPI anchor biosynthetic process|protein O-linked glycosylation|protein N-linked glycosylation dolichyl-phosphate beta-D-mannosyltransferase activity mitochondrial outer membrane|mitochondrion|endoplasmic reticulum|nuclear outer membrane-endoplasmic reticulum membrane network YPL038W MET31 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.03698318702191 0.0322121107557445 322 Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication regulation of sulfur amino acid metabolic process|regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YOR026W BUB3 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0369091248104425 0.0325851724657506 323 Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p mitotic cell cycle G2/M transition decatenation checkpoint|mitotic cell cycle spindle assembly checkpoint ubiquitin binding mitotic checkpoint complex|condensed nuclear chromosome kinetochore YNL196C_p SLZ1 hom 0.0369019740752166 0.0325929371362945 324 Sporulation-specific protein with a leucine zipper motif biological_process molecular_function cellular_component YDR160W SSY1 het FT PLASMA MEMBRANE 0.0368619073642962 0.0327822348452103 325 Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes response to amino acid stimulus molecular_function integral to membrane|plasma membrane YGL048C RPT6 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0368347427255609 0.032911110482922 326 ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of sequence-specific DNA binding transcription factor activity|nucleotide-excision repair|proteasome regulatory particle assembly|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of sequence-specific DNA binding transcription factor activity|chromatin remodeling ATPase activity|protein domain specific binding proteasome regulatory particle, base subcomplex|proteasome storage granule|nucleus YDR416W SYF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0368033357708593 0.033060652866456 327 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans generation of catalytic spliceosome for first transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 1 spliceosome|U2-type catalytic step 2 spliceosome YOL137W BSC6 hom FT GOLGI APPARATUS 0.0367431700166429 0.0333487526020233 328 Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function COPI-coated vesicle|integral to membrane|clathrin-coated vesicle YDR265W PEX10 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0366696322266645 0.0337037969929254 329 Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders protein import into peroxisome matrix|protein ubiquitination ubiquitin-protein ligase activity|protein binding peroxisomal membrane YHR086W NAM8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0366483326553323 0.0338072340109603 330 RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function mRNA splicing, via spliceosome|positive regulation of mRNA splicing, via spliceosome|mRNA splice site selection mRNA binding|RNA binding commitment complex|U1 snRNP|U2-type prespliceosome YDL101C DUN1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0365913692676123 0.0340851991195452 331 Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair protein phosphorylation|replication fork protection|cell cycle checkpoint|DNA damage checkpoint protein kinase activity nucleus YGL141W HUL5 hom FT PROTEOLYSIS|NUCLEUS 0.036581613896017 0.0341329978662342 332 Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD ER-associated protein catabolic process|protein polyubiquitination ubiquitin-ubiquitin ligase activity proteasome complex|nucleus|cytoplasm YLR332W MID2 hom FT RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0365769609632996 0.0341558161720689 333 O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|cell morphogenesis involved in conjugation|response to acid|response to osmotic stress transmembrane signaling receptor activity integral to plasma membrane|mating projection tip YBR043C QDR3 hom FT PLASMA MEMBRANE 0.0365437900857791 0.0343188663838725 334 Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin drug transmembrane transport|polyamine homeostasis drug transmembrane transporter activity|polyamine transmembrane transporter activity integral to membrane|plasma membrane YGR017W_p YGR017W hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.036294096869365 0.0356777810597208 335 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus|cytoplasm YCR060W TAH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0362741797325267 0.0356689560789231 336 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|box C/D snoRNP assembly chaperone binding R2TP complex|nucleus|cytoplasm YOR183W_p FYV12 hom 0.0362433737534964 0.0358260635449614 337 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cellular_component YNR016C ACC1 het FF|FT PROTEIN LOCALIZATION|KETONE METABOLISM|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.036216990072143 0.0359610863057857 338 Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; nuclear envelope organization|protein import into nucleus|fatty acid biosynthetic process biotin carboxylase activity|acetyl-CoA carboxylase activity mitochondrion|endoplasmic reticulum membrane YJL032W_d YJL032W het 0.0361193825683879 0.0364643851539487 339 Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Unknown Unknown Unknown YOL163W_p YOL163W hom 0.0361033679232873 0.0365475326424381 340 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YOR076C SKI7 hom FT TRANSLATION|NUCLEOTIDE METABOLISM 0.036053391731277 0.0368080462518563 341 Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay|nonfunctional rRNA decay protein binding, bridging Ski complex|cytoplasmic exosome (RNase complex)|cytoplasm YER174C GRX4 hom FT ION HOMEOSTASIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0359512506347579 0.0373454029563791 342 Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 cellular iron ion homeostasis|cellular response to oxidative stress|actin cytoskeleton organization disulfide oxidoreductase activity nucleus YGR114C_d YGR114C het FF 0.0359442921928782 0.0377819490913847 343 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Unknown Unknown Unknown YDR257C RKM4 hom FT NUCLEUS 0.0359370183224853 0.0374208052929687 344 Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity nucleus YKL097C_d YKL097C hom 0.0359314422123203 0.0374788188206958 345 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Unknown Unknown Unknown YNL254C RTC4 hom FT NUCLEUS 0.0359284161233334 0.0374664422359539 346 Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YKL114C APN1 hom FF|FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0359057276916209 0.037587037541871 347 Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine base-excision repair 3'-tyrosyl-DNA phosphodiesterase activity|DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity mitochondrion|nucleus YPL239W YAR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS 0.0358333994430805 0.0379736883679291 348 Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock ribosomal small subunit biogenesis|cellular response to oxidative stress|response to osmotic stress molecular_function cytoplasm YBR286W APE3 hom FT PROTEOLYSIS 0.0357862493235129 0.0382275588181008 349 Vacuolar aminopeptidase Y, processed to mature form by Prb1p vacuolar protein catabolic process aminopeptidase activity fungal-type vacuole YDR216W ADR1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|PEROXISOME ORGANIZATION|NUCLEUS 0.0357179904500159 0.03859763977954 350 Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization negative regulation of chromatin silencing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|chromatin organization|regulation of transcription by chromatin organization|positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by oleic acid|cellular response to oleic acid|peroxisome organization|positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|TFIIB-class transcription factor binding|sequence-specific DNA binding|TFIID-class transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription nucleus YPR193C HPA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION 0.0357123747660034 0.0386572382709482 351 Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity protein homotetramerization|histone acetylation histone acetyltransferase activity|protein homodimerization activity cytoplasm YGR160W_d YGR160W hom 0.0356836209604711 0.0387851308675704 352 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR057C LST7 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.0355264926060189 0.0396521719092739 353 Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface intracellular protein transport|vesicle-mediated transport|protein transport|Golgi to plasma membrane transport protein transporter activity vesicle coat YKL007W CAP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0355117173092836 0.0397345419256703 354 Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YGR053C_p YGR053C hom 0.0354186014960529 0.0403464782620969 355 Putative protein of unknown function biological_process molecular_function cellular_component YGL224C SDT1 hom 0.0353976393153565 0.0403754087871065 356 Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives pyrimidine nucleobase metabolic process nucleotidase activity cellular_component YPL033C SRL4 hom FT NUCLEOTIDE METABOLISM 0.0353487670907895 0.0406526316900936 357 Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p deoxyribonucleoside triphosphate metabolic process molecular_function cellular_component YML100W-A_p YML100W-A hom 0.0353372100435531 0.0407184231764101 358 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YER003C PMI40 het FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEUS 0.0352872341601693 0.0410039632357612 359 Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation protein glycosylation|cell wall mannoprotein biosynthetic process|GDP-mannose biosynthetic process mannose-6-phosphate isomerase activity nucleus|cytoplasm YEL006W YEA6 hom FT MITOCHONDRION 0.0352159174569763 0.0414143706936499 360 Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog transport|NAD transmembrane transport transporter activity|NAD transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YGR094W VAS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0352069440221668 0.0414662557201311 361 Mitochondrial and cytoplasmic valyl-tRNA synthetase valyl-tRNA aminoacylation valine-tRNA ligase activity mitochondrion|cytoplasm YDR220C_d YDR220C hom 0.0351609472660095 0.0417330768045018 362 Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YOR307C SLY41 hom FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM 0.0350177423932572 0.0426968368368809 363 Protein involved in ER-to-Golgi transport ER to Golgi vesicle-mediated transport molecular_function integral to membrane|endoplasmic reticulum YCR082W AHC2 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0350135190003836 0.0425980991098221 364 Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex|nucleus|cytoplasm YHR172W SPC97 het FT CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0349959075994957 0.0428264062190157 365 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YLR214W FRE1 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.0349820778614143 0.0427845249574638 366 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YNL102W POL1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0349335240169372 0.0430737724818913 367 Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis RNA-dependent DNA replication|lagging strand elongation|DNA synthesis involved in DNA repair|DNA replication|DNA replication initiation|premeiotic DNA replication DNA-directed DNA polymerase activity mitochondrion|alpha DNA polymerase:primase complex|replication fork YIL144W TID3 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0349326553901895 0.043078962115287 368 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering microtubule nucleation|chromosome segregation|protein localization to kinetochore structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YIR006C PAN1 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0348684749360015 0.0434638739834806 369 Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease bipolar cellular bud site selection|endocytosis|axial cellular bud site selection|cytokinesis|actin cortical patch assembly protein binding, bridging nucleus|plasma membrane|mating projection tip|cytoplasm|actin cortical patch YGR271W SLH1 hom FT TRANSLATION|RIBOSOME 0.034747908859274 0.0441948034052857 370 Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses cytoplasmic translation|regulation of translation RNA helicase activity polysomal ribosome|cytoplasm YCR057C PWP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|NUCLEUS 0.0347068052542631 0.0446053716073523 371 Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|establishment of cell polarity|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytokinesis snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|cytoplasm|90S preribosome YGL041C_d YGL041C hom 0.0346948450957085 0.0448067395544971 372 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL009W MRT4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.034694735242912 0.04455223738747 373 Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus nuclear-transcribed mRNA catabolic process|ribosomal large subunit assembly|ribosomal large subunit biogenesis|rRNA processing LSU rRNA binding nucleoplasm|nucleolus|nucleus|preribosome, large subunit precursor YMR208W ERG12 het FF|FT LIPID METABOLISM|COFACTOR METABOLISM 0.0346542561215896 0.0447697031858884 374 Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate ergosterol biosynthetic process|isopentenyl diphosphate biosynthetic process, mevalonate pathway|farnesyl diphosphate biosynthetic process, mevalonate pathway mevalonate kinase activity cytosol YBL103C RTG3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|SIGNALING|NUCLEUS 0.0345852562248133 0.045197291015245 375 Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways mitochondrion degradation|cellular response to oleic acid|mitochondria-nucleus signaling pathway|positive regulation of transcription from RNA polymerase II promoter|transcription factor import into nucleus RNA polymerase II transcription factor binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus|cytoplasm YDR079C-A TFB5 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0345556473173078 0.0453818278748998 376 Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleus YKL216W URA1 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM 0.0345523233671403 0.0455637607293786 377 Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid 'de novo' pyrimidine nucleobase biosynthetic process dihydroorotate dehydrogenase activity extrinsic to membrane|cytoplasm YDR502C SAM2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0344956154487124 0.0457579237932985 378 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cellular_component YLR054C OSW2 hom FT CELL WALL ORG/BIOGENESIS 0.0344439452205323 0.0461162243502631 379 Protein of unknown function proposed to be involved in the assembly of the spore wall ascospore wall assembly molecular_function integral to membrane|prospore membrane|cytoplasm YLR221C RSA3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.034402274917231 0.0463479078371044 380 Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosomal large subunit assembly molecular_function nucleolus|preribosome, large subunit precursor YNL121C TOM70 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0343557600188949 0.0466442996547068 381 Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication protein import into mitochondrial matrix|protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YNR066C_p YNR066C hom 0.03428170706026 0.0471194524748128 382 Putative membrane-localized protein of unknown function biological_process molecular_function integral to membrane|membrane YIR020C_p YIR020C hom 0.0342654295761888 0.0472244389862857 383 Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YGR027C RPS25A hom FT TRANSLATION|RIBOSOME 0.0342573970109277 0.047276319845261 384 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YOR025W HST3 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0341374371446731 0.0480568364583893 385 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|histone deacetylation|chromatin silencing at telomere histone deacetylase activity|DNA binding nucleus YBR007C DSF2 hom FF 0.0341243155756787 0.0481428646305058 386 Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular_component YHL025W SNF6 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|NUCLEUS|RSC COMPLEX 0.0340930932940375 0.0483480855417616 387 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|nucleotide-excision repair|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YOR367W SCP1 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0339529429541379 0.0492783493122904 388 Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin actin filament organization|chronological cell aging actin filament binding|protein binding, bridging actin cortical patch YNL163C RIA1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM 0.0338230979818916 0.050187817668786 389 Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes mature ribosome assembly GTPase activity cytoplasm YDL212W SHR3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0337272194097172 0.0508081523997068 390 Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface protein folding|ER to Golgi vesicle-mediated transport unfolded protein binding integral to endoplasmic reticulum membrane|integral to membrane|mating projection tip YJL171C YJL171C hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0337263620487989 0.0508140379664259 391 GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion|fungal-type cell wall|plasma membrane YKL068W NUP100 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0337122872442259 0.0509107395839534 392 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p positive regulation of transcription, DNA-dependent|mRNA export from nucleus|NLS-bearing substrate import into nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|protein import into nucleus|protein targeting to nuclear inner membrane|mRNA export from nucleus in response to heat stress nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YDR006C SOK1 hom FT SIGNALING|NUCLEUS 0.0336604437127989 0.0512682628564852 393 Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 cAMP-mediated signaling molecular_function nucleus YLL053C_p YLL053C hom 0.0336012553943565 0.0516790033739608 394 Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin biological_process molecular_function integral to membrane YGL198W YIP4 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0335770319353575 0.0520580669216839 395 Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process Rab GTPase binding integral to membrane|Golgi apparatus YGR141W VPS62 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.0335523940854552 0.0520201496986254 396 Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins protein targeting to vacuole molecular_function cellular_component YNR024W MPP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0334690333004661 0.0527124286542171 397 Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes nuclear mRNA surveillance of spliceosomal pre-mRNA splicing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding ribosome|nucleus|nuclear exosome (RNase complex)|TRAMP complex YMR002W MIC17 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0334609270165906 0.0526638284772202 398 Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress aerobic respiration molecular_function nucleus|cytoplasm|mitochondrial intermembrane space YBL061C SKT5 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0334558578041746 0.052699695675918 399 Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication fungal-type cell wall chitin biosynthetic process|response to osmotic stress|cytokinesis enzyme activator activity incipient cellular bud site|cellular bud neck septin ring|cellular bud neck YAR002W NUP60 hom FT PROTEIN LOCALIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0333480990894439 0.0534669832777469 400 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 double-strand break repair|poly(A)+ mRNA export from nucleus|protein export from nucleus|regulation of protein desumoylation|intracellular mRNA localization|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|telomere tethering at nuclear periphery|mRNA export from nucleus in response to heat stress|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery|NLS-bearing substrate import into nucleus|chromosome organization|nucleocytoplasmic transport|chromatin silencing at silent mating-type cassette nucleocytoplasmic transporter activity|phospholipid binding|structural constituent of nuclear pore nuclear pore central transport channel|nuclear pore nuclear basket|integral to membrane|nuclear pore YMR095C SNO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0332029572210948 0.0545151737442646 401 Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase pyridoxine metabolic process|thiamine biosynthetic process|anti-apoptosis molecular_function cytoplasm YPR028W YOP1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0329533999788544 0.0563574746407248 402 Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress vesicle-mediated transport|endoplasmic reticulum organization|endoplasmic reticulum inheritance|nuclear pore complex assembly protein binding integral to membrane|endoplasmic reticulum|endoplasmic reticulum tubular network YMR081C ISF1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0327706620101476 0.0577391111653699 403 Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant aerobic respiration molecular_function cellular_component YLR252W_d YLR252W hom 0.0324299111861203 0.0603906154818254 404 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Unknown Unknown Unknown YGL249W ZIP2 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0323395008651498 0.0612267465038267 405 Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress synaptonemal complex assembly|synapsis|reciprocal meiotic recombination molecular_function synaptonemal complex YLR407W_p YLR407W hom 0.032332279093696 0.0611686585038263 406 Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus biological_process molecular_function cellular_component YNL204C SPS18 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.0323169056198069 0.0612919246924219 407 Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation sporulation resulting in formation of a cellular spore molecular_function cellular_component YDR458C HEH2 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.0322237308828254 0.062238486496755 408 Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle biological_process molecular_function nuclear envelope YKL018W SWD2 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0321813893276822 0.0623874283289622 409 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination mRNA 3'-end processing|termination of RNA polymerase II transcription, exosome-dependent|histone H3-K4 methylation|termination of RNA polymerase II transcription, poly(A)-coupled|telomere maintenance|snoRNA 3'-end processing|histone H3-K79 methylation histone methyltransferase activity (H3-K4 specific) mRNA cleavage and polyadenylation specificity factor complex|Set1C/COMPASS complex YBL014C RRN6 het FT NUCLEUS 0.0321704208602526 0.0624768003589458 410 Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter RNA polymerase I regulatory region DNA binding|RNA polymerase I transcription factor binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity RNA polymerase I core factor complex YBL001C ECM15 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS 0.032153348445876 0.0626552916749382 411 Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis fungal-type cell wall organization molecular_function nucleus|cytoplasm YPR138C MEP3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0321229645259532 0.0628646991926523 412 Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YIL036W CST6 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0321078185508152 0.0629889176038572 413 Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication cellular response to carbon dioxide|positive regulation of transcription from RNA polymerase II promoter by oleic acid|cellular response to oleic acid|positive regulation of transcription from RNA polymerase II promoter|DNA metabolic process sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YKR003W OSH6 hom FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0319911134456415 0.063952881791758 414 Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion sterol homeostasis|maintenance of cell polarity|endocytosis|sterol transport|exocytosis|sterol metabolic process phosphatidic acid binding|lipid binding|phosphatidylinositol-3,4-bisphosphate binding|oxysterol binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-5-phosphate binding|phosphatidylinositol-4-phosphate binding cortical endoplasmic reticulum|extrinsic to membrane|cytoplasm YDL066W IDP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0319171000242323 0.0645705065444523 415 Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes isocitrate metabolic process|glutamate biosynthetic process isocitrate dehydrogenase (NADP+) activity mitochondrial nucleoid|mitochondrion YJL200C ACO2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0319088715861441 0.0647992714172444 416 Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process aconitate hydratase activity mitochondrion YKL060C FBA1 het FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0317723191011161 0.06579289740984 417 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress gluconeogenesis|glycolysis fructose-bisphosphate aldolase activity cytosol|mitochondrion|cytoplasm YNL065W AQR1 hom FT PLASMA MEMBRANE 0.0317294817404973 0.0661582085214645 418 Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress monocarboxylic acid transport|drug transmembrane transport|amino acid export monocarboxylic acid transmembrane transporter activity|drug transmembrane transporter activity integral to membrane|plasma membrane YIL009C-A EST3 hom FT NUCLEUS|CHROMOSOME 0.0316344913671762 0.0669742344408031 419 Component of the telomerase holoenzyme, involved in telomere replication telomere maintenance via telomerase ATP-dependent DNA/RNA helicase activity|GTPase activity|telomeric DNA binding chromosome, telomeric region|nucleus|telomerase holoenzyme complex YNL190W_p YNL190W hom 0.0315218748019204 0.0679523919094962 420 Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site cellular response to water deprivation molecular_function fungal-type cell wall YMR133W REC114 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0315184056405398 0.0679827095953355 421 Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YGL063W PUS2 hom FT RNA PROCESSING|MITOCHONDRION 0.031495650041921 0.0681818510450867 422 Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion YPL170W DAP1 hom FT LIPID METABOLISM 0.0314863680610437 0.0682632181452078 423 Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis regulation of ergosterol biosynthetic process heme binding endosome|membrane YNL112W DBP2 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0314295737966482 0.0689699078211546 424 ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|rRNA processing RNA helicase activity mitochondrion|nucleus|cytoplasm YFL004W VTC2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.03142631935172 0.069040183416852 425 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion polyphosphate metabolic process|vacuolar transport|microautophagy|protein localization|vacuole fusion, non-autophagic molecular_function fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum|vacuolar transporter chaperone complex YGR113W DAM1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0313673762858409 0.0694381410901426 426 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle midzone|DASH complex|condensed nuclear chromosome kinetochore|kinetochore microtubule|spindle YJL125C GCD14 het FT RNA PROCESSING|NUCLEUS 0.0313494333403097 0.0694729059695618 427 Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression tRNA methylation tRNA (adenine-N1-)-methyltransferase activity tRNA (m1A) methyltransferase complex|nucleus YFR035C_p YFR035C hom 0.031289724536631 0.0700058573501635 428 Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YBR169C SSE2 hom 0.0312522130753106 0.0703423908838787 429 Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication protein folding|protein refolding adenyl-nucleotide exchange factor activity cytoplasm YPL047W SGF11 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0312098772198525 0.0707237988196445 430 Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation histone deubiquitination|regulation of transcription from RNA polymerase II promoter enzyme activator activity DUBm complex|nucleus|SAGA complex YAR023C_p YAR023C hom 0.0311933595367788 0.0708730672827074 431 Putative integral membrane protein, member of DUP240 gene family biological_process molecular_function cellular_component YGL149W_d YGL149W hom 0.0311901049644884 0.0709025089203729 432 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Unknown Unknown Unknown YOL053W AIM39 hom FT MITOCHONDRION 0.0311740237095248 0.07104813095873 433 Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YAR018C KIN3 hom FT PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION 0.0311043886981197 0.0716815342500028 434 Nonessential serine/threonine protein kinase; possible role in DNA damage response chromosome segregation|protein phosphorylation protein kinase activity cellular_component YLR326W_p YLR326W hom 0.031008825997305 0.0725582975632609 435 Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cellular_component YCR093W CDC39 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0309728591191058 0.0733193403324684 436 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex|nucleus|cytoplasm YEL044W IES6 hom FT NUCLEUS 0.0309401638192761 0.0733223119280012 437 Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes biological_process molecular_function nucleus YPL099C AIM43 hom FT MITOCHONDRION 0.0309168584186766 0.0734532726657023 438 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YGR107W_d YGR107W hom 0.0307506538283736 0.0749709094494004 439 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR088C SLU7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.030621501200374 0.0763337738093024 440 RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity spliceosomal complex YPL074W YTA6 hom 0.0305422905209866 0.076965465630183 441 Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress biological_process ATPase activity cell cortex YKR008W RSC4 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0305084723580825 0.077293230951585 442 Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|DNA translocase activity RSC complex YDR104C SPO71 hom FT CELL WALL ORG/BIOGENESIS 0.0304933243192487 0.0774404137910501 443 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains ascospore wall assembly molecular_function ascospore wall YDR210W_p YDR210W hom FT PLASMA MEMBRANE 0.0304663577752795 0.0777029934580146 444 Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YMR279C YMR279C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0304594646578684 0.0777702295756778 445 Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication borate transmembrane transport borate efflux transmembrane transporter activity integral to membrane YFL010C WWM1 hom FT NUCLEUS|MITOCHONDRION 0.0304585186271455 0.0786713629717643 446 WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 biological_process molecular_function mitochondrion|nucleus|cytoplasm YIL154C IMP2' hom FF|FT RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS 0.0304258984507905 0.078543900560505 447 Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat DNA repair|cellular carbohydrate metabolic process transcription coactivator activity cytoplasm YBL074C AAR2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0303792108698727 0.0785565234558609 448 Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron assembly of spliceosomal tri-snRNP molecular_function U5 snRNP YBR051W_d YBR051W hom 0.0303188415259341 0.0791522493590144 449 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Unknown Unknown Unknown YER039C HVG1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0302921240247166 0.0794170679785061 450 Protein of unknown function, has homology to Vrg4p biological_process molecular_function integral to membrane YBR200W-A_p YBR200W-A hom 0.0302844679493023 0.0794930860878407 451 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YBR019C GAL10 hom FT CARBOHYDRATE METABOLISM 0.0302633417192443 0.0797031577670662 452 UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers galactose catabolic process via UDP-galactose aldose 1-epimerase activity|UDP-glucose 4-epimerase activity cytosol YDR108W TRS85 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|GOLGI APPARATUS 0.0302447197080513 0.0798887022072969 453 Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role meiosis|vesicle organization|piecemeal microautophagy of nucleus|ER to Golgi vesicle-mediated transport|peroxisome degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway molecular_function TRAPP complex|pre-autophagosomal structure YOR260W GCD1 het FT TRANSLATION 0.0302044322448037 0.0803364327614342 454 Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation translation initiation factor activity|guanyl-nucleotide exchange factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YJL013C MAD3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS 0.0302035581771483 0.0803000690889911 455 Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover distributive segregation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex YKL151C YKL151C hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0301698522048496 0.080773956832833 456 NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress nicotinamide nucleotide metabolic process ATP-dependent NAD(P)H-hydrate dehydratase activity cytoplasm YDR435C PPM1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS 0.030080216620307 0.0816796284507652 457 Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits cellular protein complex assembly|C-terminal protein methylation protein C-terminal leucine carboxyl O-methyltransferase activity cellular_component YNL189W SRP1 het FT PROTEIN LOCALIZATION|NUCLEUS 0.0300726688647364 0.0817562648603156 458 Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation protein targeting to membrane|protein import into nucleus protein transporter activity integral to membrane|nuclear exosome (RNase complex)|nucleus|cytoplasm YCL009C ILV6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0300090355803435 0.0822674514400988 459 Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria branched-chain amino acid biosynthetic process enzyme regulator activity|acetolactate synthase activity mitochondrial nucleoid|acetolactate synthase complex|mitochondrion YAL053W FLC2 hom FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0299824008367177 0.0825398479890044 460 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication protein folding|fungal-type cell wall biogenesis|FAD transport|transmembrane transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|cytoplasm YDR145W TAF12 het FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.0299775724371528 0.0825893067426206 461 Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter TBP-class protein binding|chromatin binding|RNA polymerase II activating transcription factor binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YNL119W NCS2 hom FT RNA PROCESSING 0.0299640266186795 0.0831418883362301 462 Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA wobble position uridine thiolation|pseudohyphal growth|protein urmylation|invasive growth in response to glucose limitation molecular_function cytosol|cytoplasm YNL105W_d RRT16 hom 0.0299454135728692 0.0829193325585541 463 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Unknown Unknown Unknown YLR259C HSP60 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0299179505067167 0.083202013282968 464 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated protein refolding|protein stabilization|chaperone-mediated protein complex assembly|'de novo' protein folding|protein maturation|protein import into mitochondrial intermembrane space unfolded protein binding|DNA replication origin binding|single-stranded DNA binding|ATPase activity|chaperone binding mitochondrial nucleoid|mitochondrion YGR183C QCR9 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0298978740007831 0.0835935624691926 465 Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex iron-sulfur cluster assembly|mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YDL053C PBP4 hom FT NUCLEUS 0.0298737293831278 0.0836588262005624 466 Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasmic stress granule|cytoplasm YLR036C_p YLR036C hom 0.0298657403353461 0.0837415707420106 467 Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein biological_process molecular_function integral to membrane YBL093C ROX3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0298353022436955 0.0842889719320494 468 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme negative regulation of transcription from RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex|nucleus YIL110W HPM1 hom FT NUCLEUS 0.029809082125043 0.0843766295989323 469 AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance peptidyl-histidine methylation, to form tele-methylhistidine S-adenosylmethionine-dependent methyltransferase activity|protein-histidine N-methyltransferase activity nucleus|cytoplasm YLR288C MEC3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0297338251614731 0.0851174496944616 470 DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 telomere maintenance via recombination|DNA damage checkpoint|DNA metabolic process|chromatin silencing at telomere DNA binding nucleus|checkpoint clamp complex YBR052C RFS1 hom FT OXIDATION-REDUCTION PROCESS 0.0297265031725088 0.0851943503329031 471 Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication biological_process molecular_function membrane raft|cytoplasm YGR106C VOA1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0296918919673701 0.0857923680952283 472 Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval protein complex assembly molecular_function fungal-type vacuole membrane|endoplasmic reticulum membrane YDL070W BDF2 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0295899467629684 0.0868270743630721 473 Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress negative regulation of heterochromatin assembly histone acetyl-lysine binding|core promoter binding|histone binding|TFIID-class transcription factor binding nucleus YOL162W_p YOL162W hom 0.0295616326416685 0.0869408520199272 474 Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YGR136W LSB1 hom FT NUCLEUS 0.0295245218570885 0.0874796390171896 475 Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YNL273W TOF1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0294439482767303 0.0883950775567671 476 Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase DNA repair|DNA replication checkpoint|maintenance of DNA repeat elements|replication fork arrest|replication fork protection|DNA replication|mitotic sister chromatid cohesion molecular_function nuclear chromosome|replication fork protection complex|nuclear replication fork YOR027W STI1 hom 0.0294262711784099 0.0883962558373621 477 Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding Hsp70 protein binding|Hsp90 protein binding|mRNA binding|ATPase inhibitor activity cytoplasm YPL267W ACM1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS 0.0293858367934988 0.0888347933505887 478 Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle ubiquitin-protein ligase inhibitor activity nucleus|cytoplasm YER112W LSM4 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0293806237364122 0.0889391109643601 479 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|cytoplasmic mRNA processing body assembly U6 snRNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YJL039C NUP192 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0293552804696348 0.0896939052964126 480 Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 nucleocytoplasmic transport|nuclear pore organization structural constituent of nuclear pore integral to membrane|nuclear pore nuclear basket|nuclear pore|nuclear pore inner ring YHR185C PFS1 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS|CYTOSKELETON 0.0293309918799381 0.0894324209860548 481 Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation ascospore wall assembly molecular_function cellular_component YFL063W_d YFL063W hom 0.0293232303252307 0.0897087338337199 482 Dubious open reading frame, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR211W DML1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0291427529554091 0.0915082546752998 483 Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family plasmid maintenance|mitochondrial genome maintenance molecular_function mitochondrion|cytoplasm YML032C RAD52 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0290361735306199 0.092700631467459 484 Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis postreplication repair|telomere maintenance via recombination|meiotic joint molecule formation|meiotic DNA recombinase assembly|DNA recombinase assembly|DNA strand renaturation|double-strand break repair via synthesis-dependent strand annealing|double-strand break repair via single-strand annealing|double-strand break repair via break-induced replication recombinase activity nuclear chromosome|nucleus YMR229C RRP5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0289552782980152 0.0936629155282367 485 RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding small-subunit processome|nucleolus|90S preribosome YLR373C VID22 hom FT PROTEIN LOCALIZATION|NUCLEUS|PLASMA MEMBRANE 0.028897769755566 0.0942676222446112 486 Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles vacuolar protein catabolic process molecular_function integral to plasma membrane YBR012C_d YBR012C hom 0.0288857486697384 0.0944047193785859 487 Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Unknown Unknown Unknown YML009C MRPL39 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.028883423252879 0.0944312585412369 488 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YHR187W IKI1 hom FT RNA PROCESSING|NUCLEUS 0.0288615039330561 0.094681709829875 489 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin tRNA modification tRNA binding|ATPase activity Elongator holoenzyme complex|nucleus|cytoplasm YMR086W SEG1 hom 0.0288315149548322 0.0950252270904174 490 Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes biological_process molecular_function eisosome|ribosome|cytoplasm YOR086C TCB1 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.0287924943937953 0.0954736924665895 491 Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact biological_process lipid binding mitochondrion|plasma membrane|cortical endoplasmic reticulum YLR371W ROM2 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|CELL WALL ORG/BIOGENESIS|NUCLEOTIDE METABOLISM|SITE OF POLARIZED GROWTH 0.0287653883078184 0.0957862201154588 492 GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p regulation of fungal-type cell wall organization|positive regulation of endocytosis|establishment of cell polarity|small GTPase mediated signal transduction|negative regulation of cAMP-mediated signaling|activation of Rho GTPase activity phosphatidylinositol-4,5-bisphosphate binding|Rho guanyl-nucleotide exchange factor activity|signal transducer activity incipient cellular bud site|mating projection tip|cellular bud tip YBR276C PPS1 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0287263921044045 0.0962372738151975 493 Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle inactivation of MAPK activity|protein dephosphorylation|regulation of S phase of mitotic cell cycle protein tyrosine/serine/threonine phosphatase activity cellular_component YIR018C-A_p YIR018C-A hom 0.0287258227841576 0.0962438714783988 494 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YMR176W ECM5 hom FT CELL WALL ORG/BIOGENESIS|NUCLEUS|CHROMOSOME 0.0286797729569179 0.0967787268171506 495 Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress fungal-type cell wall organization molecular_function nucleus|Snt2C complex YKL053W_d YKL053W hom 0.0286699915890917 0.0969424686940162 496 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Unknown Unknown Unknown YPR179C HDA3 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0286109397649543 0.097582631956849 497 Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p negative regulation of transcription from RNA polymerase II promoter|chromosome segregation|gene silencing by RNA|gene silencing involved in chronological cell aging|histone deacetylation histone deacetylase activity|chromatin binding|DNA binding nucleus|HDA1 complex YER062C HOR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0286075760033844 0.0976220537445573 498 Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication cellular carbohydrate metabolic process|glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YDL004W ATP16 het FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.028581233263442 0.0979312190223724 499 Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, central stalk YBR280C SAF1 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.028572277425575 0.0980866317984757 500 F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity SCF ubiquitin ligase complex YKL050C YKL050C hom 0.0285486995089913 0.09831412226086 501 Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p biological_process molecular_function cellular_component YPL122C TFB2 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0285243475022203 0.0986015124833371 502 Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter double-stranded DNA binding|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YIR036C_p IRC24 hom FT OXIDATION-REDUCTION PROCESS 0.0285132101222643 0.0987331735302086 503 Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci biological_process molecular_function integral to membrane|cytoplasm YCL024W KCC4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0284580207425746 0.0993876686379804 504 Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication budding cell bud growth|protein phosphorylation|septin checkpoint|septin ring assembly phosphatidic acid binding|phosphatidylinositol-4,5-bisphosphate binding|phosphatidylserine binding|protein kinase activity incipient cellular bud site|cellular bud neck septin collar|plasma membrane|cellular bud neck|septin ring YOR338W_p YOR338W hom FT TRANSCRIPTION FROM RNA POL II 0.0281767620952993 0.102777057074997 505 Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YMR276W DSK2 hom FT CELL CYCLE|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|NUCLEUS 0.0281681389447317 0.102882406986571 506 Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress ER-associated protein catabolic process|spindle pole body duplication associated with nuclear envelope protein binding, bridging nucleus YCL054W SPB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0281226343654505 0.103491255977364 507 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA methylation rRNA (uridine-2'-O-)-methyltransferase activity|rRNA (guanine) methyltransferase activity nucleolus|nucleus|preribosome, large subunit precursor YBL015W ACH1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0280744192867474 0.104032934287381 508 Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth acetate metabolic process acetate CoA-transferase activity|acetyl-CoA hydrolase activity cytosol|mitochondrion YBR196C PGI1 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0280704879501868 0.104236453162164 509 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation gluconeogenesis|pentose-phosphate shunt|glycolysis glucose-6-phosphate isomerase activity cytosol|mitochondrion|plasma membrane YPL036W PMA2 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0280531841259604 0.104295037288586 510 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential proton transport|regulation of pH hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YLR316C TAD3 het FT RNA PROCESSING|NUCLEUS 0.0280520960091177 0.104308481889716 511 Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YLL044W_d YLL044W hom 0.0280455287374662 0.104389655284349 512 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Unknown Unknown Unknown YDR203W_d YDR203W hom FF 0.0280166746731857 0.104850547472854 513 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR087W_d VPS69 hom 0.0279837480005265 0.10536355651535 514 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Unknown Unknown Unknown YKL092C BUD2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0279440996614141 0.105649727294969 515 GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint bipolar cellular bud site selection|small GTPase mediated signal transduction|filamentous growth|cellular bud site selection|axial cellular bud site selection GTPase activator activity|Ras GTPase activator activity intracellular YOR316C COT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0279426433544956 0.105667906702737 516 Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication cellular zinc ion homeostasis|cobalt ion transport|zinc ion transport cobalt ion transmembrane transporter activity|zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|fungal-type vacuole YFR025C HIS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0279201824572437 0.106000708880134 517 Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control histidine biosynthetic process histidinol-phosphatase activity intracellular YDL188C PPH22 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME 0.0278967440910195 0.106451014528993 518 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication actin filament organization|budding cell bud growth|G1/S transition of mitotic cell cycle|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity condensed nuclear chromosome, centromeric region YPL201C YIG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0278836518208812 0.106406398839447 519 Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol glycerol biosynthetic process molecular_function cytosol|nucleus YJR032W CPR7 hom 0.0278464874473409 0.106873741153123 520 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity protein refolding|response to stress unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytosol YIL145C PAN6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0277973760629504 0.107703869279779 521 Pantothenate synthase, also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC pantothenate biosynthetic process pantoate-beta-alanine ligase activity nucleus|cytoplasm YBR269C_p FMP21 hom FT MITOCHONDRION 0.02778489450035 0.107651851370356 522 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOL164W BDS1 hom 0.0277655401850563 0.108160403520152 523 Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources dodecyl sulfate metabolic process arylsulfatase activity|alkyl sulfatase activity cellular_component YIL149C MLP2 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CYTOSKELETON 0.0277436654352699 0.108175203067177 524 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length poly(A)+ mRNA export from nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|regulation of transcription, DNA-dependent|telomere tethering at nuclear periphery|spindle pole body organization|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery ribonucleoprotein complex binding nucleoplasm|nuclear envelope|mitochondrion|ribonucleoprotein complex|integral to membrane|spindle pole body YPL251W_d YPL251W het 0.0277082581399357 0.108626259419288 525 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Unknown Unknown Unknown YGL147C RPL9A hom FT TRANSLATION|RIBOSOME 0.0276936858586297 0.108812328348343 526 Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YMR194C-B CMC4 hom FT MITOCHONDRION 0.0276677697395416 0.10914386574813 527 Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs biological_process molecular_function mitochondrial intermembrane space YDR429C TIF35 het FF|FT TRANSLATION 0.0275771611041462 0.110309275227933 528 eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity eukaryotic translation initiation factor 3 complex YPR054W SMK1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.0275610797583474 0.110517136683036 529 Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p ascospore wall assembly|protein phosphorylation|negative regulation of sporulation resulting in formation of a cellular spore MAP kinase activity mitochondrion YOR359W VTS1 hom FT PROTEIN LOCALIZATION|RNA PROCESSING|NUCLEUS 0.0275523440943602 0.110683386560807 530 Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity nuclear-transcribed mRNA catabolic process|positive regulation of endodeoxyribonuclease activity|nuclear-transcribed mRNA poly(A) tail shortening flap-structured DNA binding|RNA binding cytosol|nucleus|cytoplasmic mRNA processing body YKL027W YKL027W hom FT MITOCHONDRION 0.0275227362036394 0.111067293486467 531 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion YIL084C SDS3 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0274993624967299 0.111531369434345 532 Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of chromatin silencing at telomere|chromatin silencing|negative regulation of chromatin silencing at rDNA|positive regulation of transcription from RNA polymerase II promoter|histone deacetylation histone deacetylase activity Rpd3L-Expanded complex|Rpd3L complex YMR319C FET4 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0274688077028531 0.112090574044962 533 Low-affinity Fe(II) transporter of the plasma membrane intracellular copper ion transport|zinc ion transport|low-affinity iron ion transport|copper ion import copper ion transmembrane transporter activity|iron ion transmembrane transporter activity integral to membrane|plasma membrane|integral to plasma membrane YBR141C_p YBR141C hom FT NUCLEUS 0.027459728470784 0.111834289488724 534 Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity nucleolus YAL068C PAU8 hom FT TRANSCRIPTION FROM RNA POL II 0.0274545427842999 0.111955511566338 535 Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YJL165C HAL5 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0274322668272267 0.112730212846223 536 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication cellular cation homeostasis|protein phosphorylation|cellular protein localization|protein dephosphorylation protein kinase activity cellular_component YBR056W-A_p YBR056W-A hom 0.0273789716233802 0.112892633853971 537 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B biological_process molecular_function cellular_component YKR072C SIS2 hom FF|FT CELL CYCLE|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0273587436192632 0.113374259670955 538 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis regulation of mitotic cell cycle|response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex|nucleus|cytoplasm YAL019W FUN30 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0273495030675028 0.11328078777288 539 Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate chromosome organization|chromatin silencing at rDNA|heterochromatin maintenance involved in chromatin silencing|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette|heterochromatin assembly involved in chromatin silencing chromatin binding|DNA-dependent ATPase activity|DNA binding|protein homodimerization activity mitochondrion|mating-type region heterochromatin|nucleus YCR015C_p YCR015C hom 0.0272675664211263 0.114365565753201 540 Putative protein of unknown function; YCR015C is not an essential gene biological_process molecular_function cellular_component YLR172C DPH5 hom 0.0272221224511336 0.115133368837809 541 Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-diphthamide biosynthetic process from peptidyl-histidine diphthine synthase activity cytoplasm YPL049C DIG1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0272199855077683 0.114999241122719 542 MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown DNA binding|transcription factor binding nucleus YDR424C DYN2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0272143546106618 0.115074414883464 543 Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex microtubule-based process|actin filament-based process|nuclear pore complex assembly molecular_function cytoplasmic dynein complex|cytoplasmic microtubule|peroxisome|nuclear pore YLL057C JLP1 hom FT OXIDATION-REDUCTION PROCESS 0.0271906839626495 0.115390846080414 544 Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation sulfur compound catabolic process sulfonate dioxygenase activity cellular_component YLR147C SMD3 het FT RNA PROCESSING|NUCLEUS 0.0270945421834846 0.11701091683777 545 Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YOR160W MTR10 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|NUCLEUS 0.0269578424838415 0.118540058358394 546 Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress RNA localization|tRNA transport|RNA import into nucleus|protein import into nucleus|mRNA-binding (hnRNP) protein import into nucleus nuclear localization sequence binding integral to membrane|nucleus|cytoplasm YBR049C REB1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0269470931812238 0.11868705496891 547 RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication termination of RNA polymerase I transcription|regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nucleoplasm YOL016C CMK2 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.0269311973063019 0.118904692678224 548 Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YPR156C TPO3 hom FT PLASMA MEMBRANE 0.0268901459666531 0.119578546066615 549 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication spermine transport spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|plasma membrane YMR170C ALD2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.02687853716189 0.11962792123132 550 Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p cellular aldehyde metabolic process|beta-alanine biosynthetic process|polyamine catabolic process aldehyde dehydrogenase (NAD) activity cytoplasm YNL064C YDJ1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION 0.0268292681432261 0.120307691058957 551 Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family protein refolding|ER-associated protein catabolic process|protein targeting to mitochondrion|protein targeting to ER|'de novo' protein folding unfolded protein binding|ATPase activator activity cytosol|TRC complex|perinuclear region of cytoplasm YPL133C RDS2 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0267366907013169 0.121593163968253 552 Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|cellular response to xenobiotic stimulus|positive regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YLR289W GUF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION|RIBOSOME 0.0267283517007629 0.121709479243166 553 Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor positive regulation of translation ribosome binding|GTPase activity mitochondrion|mitochondrial ribosome|mitochondrial matrix YGL003C CDH1 hom FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0266783590833137 0.122520204896232 554 Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p activation of mitotic anaphase-promoting complex activity|negative regulation of spindle pole body separation|positive regulation of mitotic metaphase/anaphase transition|Unknown|regulation of cell size anaphase-promoting complex binding|cyclin binding|ubiquitin-protein ligase activator activity nucleus|anaphase-promoting complex|cytoplasm YNL161W CBK1 het FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0266687253858414 0.122543703464684 555 Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress regulation of fungal-type cell wall organization|establishment or maintenance of cell polarity|protein phosphorylation|establishment or maintenance of actin cytoskeleton polarity|budding cell apical bud growth|Unknown protein serine/threonine kinase activity incipient cellular bud site|cellular bud|cellular bud neck|cellular bud tip|cytoplasm|cell cortex|nucleus|mating projection tip YGR178C PBP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS|MITOCHONDRION 0.0266624196152828 0.1226321871753 556 Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress positive regulation of translation|mRNA polyadenylation|stress granule assembly molecular_function mitochondrion|nucleus|polysome|cytoplasmic stress granule|cytoplasm YML047W-A_d YML047W-A hom 0.0266588964055714 0.122961108066081 557 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFL052W_p YFL052W hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0266563314512335 0.122717664725171 558 Putative zinc cluster protein that contains a DNA binding domain; computational analysis suggests a role as a transcription factor; null mutant is sensitive to Calcofluor White, low osmolarity, and heat, suggesting a role for YFL052Wp in cell wall integrity biological_process DNA binding cellular_component YLL015W BPT1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS 0.0266269796030087 0.123130416353584 559 ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p cadmium ion transport|bilirubin transport bilirubin transmembrane transporter activity|cadmium ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YKL150W MCR1 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0266064556494718 0.123587718594863 560 Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis ergosterol biosynthetic process|cellular response to oxidative stress cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane|mitochondrial intermembrane space YIR028W DAL4 hom 0.0265613375998204 0.124057408651697 561 Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process|allantoin transport allantoin uptake transmembrane transporter activity integral to membrane|membrane YAL042C-A_d YAL042C-A hom 0.0265602373628062 0.124072992383795 562 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Unknown Unknown Unknown YJL062W LAS21 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0265133671811767 0.124738279531252 563 Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity integral to endoplasmic reticulum membrane|integral to membrane|integral to plasma membrane YOR275C RIM20 hom FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS|NUCLEUS 0.0264957343815811 0.124989282523546 564 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation proteolysis|response to pH|protein processing|invasive growth in response to glucose limitation|sporulation resulting in formation of a cellular spore molecular_function nucleus|cytoplasm YFL045C SEC53 het FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM 0.0264657048685173 0.126039535362083 565 Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen protein targeting to ER phosphomannomutase activity cytosol YKL197C PEX1 hom FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION 0.0264499185118121 0.125643311757596 566 AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis protein import into peroxisome matrix, receptor recycling ATPase activity|protein heterodimerization activity peroxisomal membrane|peroxisome YDR131C_p YDR131C hom FT PROTEOLYSIS 0.0264482468431581 0.125893167560031 567 F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YHR164C DNA2 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0264482208930644 0.125667596664167 568 Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress DNA repair|lagging strand elongation|meiotic DNA double-strand break processing|DNA strand renaturation|replicative cell aging|telomere maintenance|DNA-dependent DNA replication ATP-dependent DNA helicase activity|single-stranded DNA specific endodeoxyribonuclease activity|5'-flap endonuclease activity nuclear chromosome, telomeric region|nucleus|cytoplasm YOR063W RPL3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0264086165326899 0.126235186106814 569 Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YBR115C LYS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0263615805507947 0.126911871875502 570 Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p lysine biosynthetic process via aminoadipic acid L-aminoadipate-semialdehyde dehydrogenase activity cytoplasm YMR010W_p YMR010W hom 0.0263552647533195 0.127002948947211 571 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function integral to membrane|cytoplasm YGL035C MIG1 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0263257290542488 0.127429544039034 572 Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion negative regulation of transcription from RNA polymerase II promoter by glucose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|nuclear envelope lumen|cytoplasm YOR016C ERP4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0262919274489951 0.127919119541364 573 Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport ER to Golgi vesicle-mediated transport molecular_function integral to membrane YJL145W SFH5 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0262277840753992 0.128852177076842 574 Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Golgi to plasma membrane protein transport|phosphatidylinositol metabolic process|Unknown|regulation of exocytosis|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cell periphery|cytosol|plasma membrane|cortical endoplasmic reticulum YDR468C TLG1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS 0.0262167839729272 0.129012718990035 575 Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p endocytosis|vesicle fusion SNAP receptor activity trans-Golgi network|SNARE complex|endosome YDR511W ACN9 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0261827434253112 0.129796797688104 576 Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes carbon utilization|regulation of gluconeogenesis molecular_function mitochondrion|mitochondrial intermembrane space YOR139C_d YOR139C hom 0.0261401543550153 0.130135409684097 577 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Unknown Unknown Unknown YCR020C PET18 hom 0.0261388295827728 0.130154885185367 578 Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin thiamine metabolic process molecular_function cytosol YLR219W MSC3 hom FT CELL CYCLE|PLASMA MEMBRANE 0.0261319301385956 0.130256350594626 579 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress reciprocal meiotic recombination molecular_function cellular_component YDR437W GPI19 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0261214160192618 0.130411092539463 580 Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|nuclear envelope|integral to endoplasmic reticulum membrane|endoplasmic reticulum YJL100W LSB6 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0260677598654034 0.131203000273838 581 Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity fungal-type vacuole membrane|plasma membrane|cytoplasm YKR087C OMA1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0260111796432186 0.132042095929434 582 Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes misfolded or incompletely synthesized protein catabolic process metalloendopeptidase activity mitochondrion|mitochondrial inner membrane YNL260C LTO1 het FT NUCLEUS 0.0259961342630333 0.132265919848464 583 Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species biological_process molecular_function nucleus|cytoplasm YNL276C_d YNL276C hom 0.0257154205263895 0.136496066096382 584 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Unknown Unknown Unknown YDR132C YDR132C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS|PLASMA MEMBRANE 0.0256230128940023 0.137911180268109 585 Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YKL204W EAP1 hom FT TRANSLATION 0.0256222827432437 0.137922406506772 586 eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade negative regulation of translation eukaryotic initiation factor 4E binding mRNA cap binding complex|cytoplasmic stress granule YER117W RPL23B hom FT TRANSLATION|RIBOSOME 0.0255385335557499 0.139510021985771 587 Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL117W CYK3 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0255142956884977 0.139590497848507 588 SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants barrier septum assembly involved in cell cycle cytokinesis|cytokinesis molecular_function cellular bud neck contractile ring|cellular bud neck|cytoplasm YPL183C RTT10 hom FT VESICLE-MEDIATED TRANSPORT 0.0255092376706335 0.139846329661334 589 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endocytic recycling molecular_function endosome|cytoplasm YBR033W_p EDS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.025505286104809 0.139730368637722 590 Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication biological_process sequence-specific DNA binding cellular_component YLL038C ENT4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0253849417406139 0.141609027845395 591 Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome endocytosis|actin filament organization|establishment of protein localization to plasma membrane|actin cortical patch assembly clathrin binding actin cortical patch YPR185W ATG13 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0253610604494548 0.142281821132044 592 Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|activation of protein kinase activity|CVT pathway protein kinase regulator activity Atg1p signaling complex|extrinsic to membrane YGL092W NUP145 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0252869166882135 0.143153552680936 593 Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 double-strand break repair|poly(A)+ mRNA export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|tRNA export from nucleus|protein targeting to nuclear inner membrane|telomere tethering at nuclear periphery|nuclear pore distribution|positive regulation of transcription, DNA-dependent|NLS-bearing substrate import into nucleus|protein import into nucleus nucleocytoplasmic transporter activity|RNA binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore|nuclear pore outer ring YER116C SLX8 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0252411276950457 0.143879436094463 594 Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress protein sumoylation|telomere maintenance|response to DNA damage stimulus|protein ubiquitination ubiquitin-protein ligase activity ubiquitin ligase complex|nucleus YEL056W HAT2 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0251965433314158 0.144588930229093 595 Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing histone acetylation|chromatin assembly or disassembly|chromatin silencing at telomere histone acetyltransferase activity|histone binding histone acetyltransferase complex|nucleus|cytoplasm YLR011W LOT6 hom FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0251574143069731 0.145213816050886 596 FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions cellular response to oxidative stress|cytoplasmic sequestering of transcription factor NAD(P)H dehydrogenase (quinone) activity|transcription factor binding cytosol|nucleus|cytoplasm YER177W BMH1 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0251441289143968 0.145426451671712 597 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|pseudohyphal growth|signal transduction involved in filamentous growth|ascospore formation|fungal-type cell wall chitin biosynthetic process|Ras protein signal transduction|DNA damage checkpoint|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle DNA replication origin binding|phosphoserine binding plasma membrane|nucleus YAL032C PRP45 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0250684379653346 0.14664245126621 598 Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene generation of catalytic spliceosome for second transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity nucleus|post-mRNA release spliceosomal complex|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YNL300W_p TOS6 hom 0.0250469038619619 0.146989820199432 599 Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid biological_process molecular_function fungal-type cell wall YGL112C TAF6 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.025030910142222 0.14724822339529 600 Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II activating transcription factor binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YDR244W PEX5 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0250207948685647 0.147411830349158 601 Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions protein import into peroxisome matrix, docking peroxisome matrix targeting signal-1 binding|protein binding, bridging cytosol|peroxisomal membrane|peroxisome YGL049C TIF4632 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RIBOSOME 0.0250088705556739 0.147604875492126 602 Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication translational initiation|stress granule assembly translation initiation factor binding|mRNA binding|translation initiation factor activity eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule YPL026C SKS1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN PHOSPHORYLATION|NUCLEUS 0.024978536131586 0.148096836241603 603 Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway glucose transport|protein phosphorylation|response to glucose stimulus|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YPR049C ATG11 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|PEROXISOME ORGANIZATION 0.0249633415559032 0.148343730594481 604 Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) piecemeal microautophagy of nucleus|mitochondrion degradation|peroxisome degradation|protein localization to pre-autophagosomal structure|CVT pathway protein complex scaffold pre-autophagosomal structure|extrinsic to membrane YOR253W NAT5 hom FT RIBOSOME 0.0249304353301369 0.148879495892802 605 Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatA complex|cytosolic ribosome YAR019C CDC15 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.024885603399426 0.149611805171362 606 Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress regulation of exit from mitosis|protein phosphorylation|meiotic anaphase II|cytokinesis protein kinase activity spindle pole body|cellular bud neck YLR180W SAM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.0248716292193724 0.14984062774173 607 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cytoplasm YDR412W RRP17 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0248170670457813 0.15073662078233 608 Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA rRNA processing molecular_function cytoplasm YOR034C-A_p YOR034C-A hom 0.0248128410428986 0.150867131148516 609 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YGR047C TFC4 het FT NUCLEUS 0.0247977609501305 0.15105463175044 610 One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YKR019C IRS4 hom FF|FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|MITOCHONDRION 0.0247690415112311 0.152079165234683 611 EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication autophagy|fungal-type cell wall organization|chromatin silencing at rDNA|inositol lipid-mediated signaling|CVT pathway|cellular response to starvation molecular_function mitochondrion|pre-autophagosomal structure YPR154W PIN3 hom FT CYTOSKELETON ORGANIZATION|NUCLEUS 0.0247406509033522 0.15199834300835 612 Protein that induces appearance of [PIN+] prion when overproduced actin cytoskeleton organization molecular_function nucleus|cytoplasm YEL015W EDC3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0247062950271461 0.152629432481748 613 Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress deadenylation-independent decapping of nuclear-transcribed mRNA|cytoplasmic mRNA processing body assembly molecular_function cytoplasmic mRNA processing body YML108W YML108W hom FT NUCLEUS 0.0246908973867567 0.152824143125674 614 Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL171W ROK1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS 0.0246861059454736 0.152903850391436 615 RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|snoRNA localization RNA-dependent ATPase activity nucleolus|90S preribosome YPL057C SUR1 hom FT LIPID METABOLISM 0.0246655403976005 0.153246324393726 616 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication glycosphingolipid biosynthetic process|mannosyl-inositol phosphorylceramide metabolic process|sphingolipid biosynthetic process mannosyltransferase activity|transferase activity, transferring glycosyl groups integral to membrane|intracellular YHR036W BRL1 het FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0246156309124556 0.154079881687188 617 Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export mRNA export from nucleus|protein export from nucleus|nuclear envelope organization molecular_function nuclear envelope|endoplasmic reticulum YDL047W SIT4 hom FT CELL CYCLE|RNA PROCESSING|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0245996426031341 0.154347635637849 618 Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization intracellular protein kinase cascade|replicative cell aging|G1/S transition of mitotic cell cycle|actin cytoskeleton organization|DNA repair|cellular response to oxidative stress|fungal-type cell wall organization|TOR signaling cascade|dephosphorylation|tRNA wobble uridine modification protein serine/threonine phosphatase activity nucleus|cytoplasm YJL010C NOP9 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0245730441548762 0.154793858828266 619 Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit export from nucleus RNA binding preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YDR025W RPS11A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0245656054713749 0.155227021791538 620 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YIL068C SEC6 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0245321792506925 0.155542996343742 621 Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p exocyst localization|Golgi to plasma membrane transport|exocytosis|vesicle fusion|negative regulation of SNARE complex assembly|vesicle docking involved in exocytosis protein homodimerization activity mating projection tip|cellular bud neck|cellular bud tip|exocyst YDR259C YAP6 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0245320544986736 0.155668520676655 622 Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding transcription factor recruiting transcription factor activity|sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding nucleus YJL019W MPS3 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0245242886267993 0.155614337513266 623 Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p meiotic telomere clustering|synapsis|nuclear migration involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope|telomere tethering at nuclear periphery|mitotic sister chromatid cohesion molecular_function nuclear envelope|nuclear periphery|integral to membrane|nuclear chromosome, telomeric region|half bridge of spindle pole body YNR052C POP2 hom FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|SITE OF POLARIZED GROWTH 0.0245012914219131 0.156002487482722 624 RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter 3'-5'-exoribonuclease activity CCR4-NOT core complex|mating projection tip|cytoplasmic mRNA processing body|cytoplasm YDR480W DIG2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0244773850577844 0.15640676026674 625 MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown transcription factor binding nucleus YJL011C RPC17 het FT NUCLEUS 0.0244762275115251 0.156426355297662 626 RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YPR048W TAH18 het FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0243847990314319 0.157979949421663 627 Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance oxidation-reduction process|iron-sulfur cluster assembly oxidoreductase activity, acting on NADH or NADPH mitochondrion YDL059C RAD59 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0243594963893537 0.158598366248962 628 Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p DNA strand renaturation|telomere maintenance via recombination|double-strand break repair via break-induced replication|double-strand break repair via single-strand annealing protein binding nucleus YJL177W RPL17B hom FT TRANSLATION|RIBOSOME 0.0243321798450774 0.158879359177296 629 Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR376C PSY3 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0242955244835934 0.159508187749509 630 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C recombinational repair|error-free translesion synthesis molecular_function nucleus|Shu complex|cytoplasm YPL032C SVL3 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|SITE OF POLARIZED GROWTH 0.0241562473838047 0.16254207764423 631 Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress endocytosis molecular_function cellular bud|cellular bud neck|cytoplasm YDL157C_p YDL157C hom FT MITOCHONDRION 0.0241383870258997 0.162225244040853 632 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YIL150C MCM10 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0241320397942772 0.162335722767646 633 Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|double-strand break repair via break-induced replication|DNA replication initiation|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette double-stranded DNA binding|protein binding|DNA replication origin binding|single-stranded DNA binding replication fork protection complex|replication fork|nucleus YBR066C NRG2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0241160252036687 0.162614721937719 634 Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter by glucose|biofilm formation|pseudohyphal growth|Unknown sequence-specific DNA binding nucleus YGR122C-A_d YGR122C-A hom 0.0240819339298781 0.163209849009741 635 Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Unknown Unknown Unknown YOL154W ZPS1 hom 0.0240760480313006 0.163312764353528 636 Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH biological_process molecular_function fungal-type cell wall|fungal-type vacuole YBR288C APM3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0240702352181185 0.16341444978363 637 Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YER186C_p YER186C hom 0.0240644351180921 0.163515960362579 638 Putative protein of unknown function biological_process molecular_function cellular_component YBR026C ETR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0240213738443633 0.164271084415298 639 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis fatty acid biosynthetic process|aerobic respiration enoyl-[acyl-carrier-protein] reductase activity mitochondrion YGR287C IMA1 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.02401002999322 0.164470446883376 640 Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family disaccharide catabolic process oligo-1,6-glucosidase activity mitochondrion|ribosome YBL042C FUI1 hom FF|FT PLASMA MEMBRANE 0.0239925998421984 0.164777127879528 641 High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress uridine transport|transmembrane transport uridine transmembrane transporter activity integral to membrane|plasma membrane YOR316C-A_p YOR316C-A hom 0.0239858914678223 0.164895275390757 642 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YMR298W LIP1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0239767055474246 0.16505716070335 643 Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis ceramide biosynthetic process sphingosine N-acyltransferase activity nuclear envelope|endoplasmic reticulum|endoplasmic reticulum membrane YDL215C GDH2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0239495745527561 0.165788652593702 644 NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels nitrogen compound metabolic process glutamate dehydrogenase (NAD+) activity mitochondrion YLR124W_d YLR124W hom 0.0239397944341016 0.16570885846108 645 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR326C_d YMR326C hom 0.0239008305622571 0.166398896888359 646 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Unknown Unknown Unknown YOR005C DNL4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.0238445102051954 0.167400127144584 647 DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth double-strand break repair via nonhomologous end joining|replicative cell aging DNA ligase (ATP) activity DNA ligase IV complex|nuclear chromatin YPL014W_p YPL014W hom FT NUCLEUS 0.0237101941173114 0.16980616948234 648 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YGR148C RPL24B hom FT TRANSLATION|RIBOSOME 0.0237080204617282 0.16984531875922 649 Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YGR281W YOR1 hom FT NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0236779329799638 0.170387913317625 650 Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) transport|response to drug xenobiotic-transporting ATPase activity integral to membrane|plasma membrane YGR268C HUA1 hom 0.023659879488976 0.170714110739455 651 Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YKL098W MTC2 hom 0.0236188018002011 0.171777910212702 652 Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YEL033W_p MTC7 hom 0.0235812101116874 0.172589598833204 653 Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant biological_process molecular_function cellular_component YDL224C WHI4 hom 0.0235630261654097 0.172472080822335 654 Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication regulation of cell size RNA binding cytoplasm YPL261C_d YPL261C hom 0.0235273526020538 0.173122985462476 655 Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Unknown Unknown Unknown YNL106C INP52 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0234545002675231 0.174779724723394 656 Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity mating projection tip|cytoplasm|actin cortical patch YKR105C_p VBA5 hom 0.0234213776237175 0.175067443337325 657 Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p biological_process molecular_function integral to membrane YOR276W CAF20 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION 0.0234165987641555 0.175155509478255 658 Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E negative regulation of translation|positive regulation of cytoplasmic mRNA processing body assembly translation regulator activity mRNA cap binding complex YKL144C RPC25 het FT NUCLEUS 0.0234116670823022 0.175246426510835 659 RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YBL073W_d YBL073W het 0.0233778889913455 0.175870082027944 660 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Unknown Unknown Unknown YMR256C COX7 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0233764331844387 0.17628427933614 661 Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YBR259W YBR259W hom 0.0233542601816353 0.176307330589872 662 Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component YDR161W_p YDR161W hom FT PROTEOLYSIS|NUCLEUS 0.0233327610629926 0.176705872782808 663 Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) ER-associated protein catabolic process molecular_function nucleus|cytoplasm YLL011W SOF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.023323551053572 0.176876809473553 664 Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YPL067C_p YPL067C hom 0.0233207552422298 0.176928723761124 665 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm YMR277W FCP1 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0233177477576099 0.177049194100814 666 Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) dephosphorylation of RNA polymerase II C-terminal domain|regulation of transcription from RNA polymerase II promoter protein serine/threonine phosphatase activity nucleus YLR057W MNL2 hom FT PROTEOLYSIS|ENDOPLASMIC RETICULUM 0.023313021340676 0.177072390554192 667 Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene ER-associated protein catabolic process molecular_function endoplasmic reticulum YJL047C-A_p YJL047C-A hom 0.0232506065387468 0.178235003454183 668 Putative protein of unknown function biological_process molecular_function cellular_component YDR120C TRM1 hom FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0232415760398685 0.178403685489792 669 tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments tRNA methylation tRNA (guanine-N2-)-methyltransferase activity mitochondrion|nuclear envelope|nuclear inner membrane YBR015C MNN2 hom FF|FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0232186635451755 0.178832204169726 670 Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YKR056W TRM2 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE 0.023184408174059 0.179539203102257 671 tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair double-strand break repair|DNA repair|tRNA modification tRNA (m5U54) methyltransferase activity|double-stranded DNA specific 5'-3' exodeoxyribonuclease activity|single-stranded DNA specific endodeoxyribonuclease activity cellular_component YJR010C-A SPC1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0231592963542465 0.179946072753644 672 Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress signal peptide processing|protein targeting to ER molecular_function signal peptidase complex YOR014W RTS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.023111269153896 0.180916018685892 673 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle spindle orientation checkpoint|cellular protein localization|meiotic sister chromatid cohesion, centromeric|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|spindle pole body|condensed nuclear chromosome, centromeric region|cellular bud neck|cytoplasm YLR049C_p YLR049C hom 0.0230927799481993 0.181200189642616 674 Putative protein of unknown function biological_process molecular_function cellular_component YGR131W FHN1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0230819070626108 0.181405804914601 675 Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain establishment of protein localization to plasma membrane molecular_function integral to membrane|membrane raft YIL021W RPB3 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0230608688254905 0.182065069193022 676 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity nucleoplasm|DNA-directed RNA polymerase II, core complex YHR030C SLT2 hom FF|FT PROTEIN LOCALIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0230515688085421 0.183421348065341 677 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway protein phosphorylation|endoplasmic reticulum unfolded protein response|signal transduction|UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|regulation of transcription factor import into nucleus|barrier septum assembly|response to acid|regulation of cell size MAP kinase activity|protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm|cellular bud tip YDL100C GET3 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0230445246360082 0.182179284221836 678 Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress retrograde vesicle-mediated transport, Golgi to ER|pheromone-dependent signal transduction involved in conjugation with cellular fusion|response to heat|protein insertion into ER membrane|response to metal ion|posttranslational protein targeting to membrane ATPase activity|guanyl-nucleotide exchange factor activity endoplasmic reticulum|GET complex YPL010W RET3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0230058266459648 0.182849393566991 679 Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER molecular_function COPI vesicle coat YOR230W WTM1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING|NUCLEUS 0.0230013815911267 0.1829993198592 680 Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats negative regulation of chromatin silencing at silent mating-type cassette|maintenance of protein location in nucleus|negative regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of chromatin silencing at telomere|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter protein binding|transcription corepressor activity nucleus YJR069C HAM1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0230006432919094 0.1829480539811 681 Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress purine deoxyribonucleoside triphosphate catabolic process|pyrimidine deoxyribonucleoside triphosphate catabolic process dITP diphosphatase activity|ITP diphosphatase activity|XTP diphosphatase activity|dCTP diphosphatase activity|UTP diphosphatase activity|dTTP diphosphatase activity|dATP pyrophosphohydrolase activity|dGTP diphosphatase activity|dUTP diphosphatase activity|GTP diphosphatase activity nucleus|cytoplasm YPL274W SAM3 hom FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0229995569659346 0.182968736202565 682 High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p spermidine transport|putrescine transport|S-adenosylmethionine transport S-adenosylmethionine transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YDR494W RSM28 hom FT TRANSLATION|MITOCHONDRION|RIBOSOME 0.0229462645614947 0.183985481648083 683 Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation cytoplasmic translation|translational initiation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YFR020W_p YFR020W hom 0.0229221297247502 0.184512808759263 684 Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YBL039C URA7 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0229213699956899 0.184789616358 685 Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YKR054C DYN1 hom FT CELL CYCLE|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0229129910255326 0.184622411845495 686 Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p nuclear migration along microtubule|establishment of mitotic spindle orientation|mitotic spindle elongation|mitotic sister chromatid segregation microtubule motor activity cytoplasmic dynein complex|cell cortex|cytoplasmic microtubule|spindle pole body|astral microtubule YKL190W CNB1 hom FF|FT SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS 0.0228766969376483 0.185319020550039 687 Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|fungal-type cell wall organization|cellular ion homeostasis calcium-dependent protein serine/threonine phosphatase activity|calcium ion binding calcineurin complex YNL031C HHT2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0228609938319517 0.185621018833548 688 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation chromatin assembly or disassembly|global genome nucleotide-excision repair|rRNA transcription|sexual sporulation resulting in formation of a cellular spore|mitotic cell cycle spindle assembly checkpoint DNA binding nuclear nucleosome YEL035C_p UTR5 het 0.0228291120762627 0.186761599023241 689 Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions biological_process molecular_function cellular_component YPL109C_p YPL109C hom FT MITOCHONDRION 0.0228217659459549 0.186377028994402 690 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOL098C_p YOL098C hom FT PROTEOLYSIS 0.022788822114453 0.187013686236587 691 Putative metalloprotease biological_process molecular_function cytoplasm YPL120W VPS30 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.0227739111258687 0.187368190131424 692 Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 phosphatidylinositol biosynthetic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|peroxisome degradation|retrograde transport, endosome to Golgi|macroautophagy|CVT pathway|late endosome to vacuole transport 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|extrinsic to membrane|cytoplasm YNR051C BRE5 hom FT VESICLE-MEDIATED TRANSPORT 0.022756496722678 0.187639950954914 693 Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A protein deubiquitination|ribophagy|regulation of ER to Golgi vesicle-mediated transport|Unknown molecular_function cytoplasm YJL080C SCP160 hom FT CELL CYCLE|SIGNALING|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0227364496817743 0.188095007893773 694 Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins meiotic telomere clustering|pheromone-dependent signal transduction involved in conjugation with cellular fusion|chromosome segregation|intracellular mRNA localization|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere G-protein alpha-subunit binding|RNA binding endoplasmic reticulum membrane|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YER093C TSC11 het FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0227218375850826 0.188313146633343 695 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|sphingolipid biosynthetic process protein binding TORC2 complex YHR193C EGD2 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.0227192132673373 0.188430122200401 696 Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes 'de novo' cotranslational protein folding unfolded protein binding|phosphatidic acid binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding nascent polypeptide-associated complex YCR102C_p YCR102C hom FT OXIDATION-REDUCTION PROCESS 0.0226854358795407 0.189815898120287 697 Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to copper ion molecular_function cellular_component YIR013C GAT4 hom FT NUCLEUS 0.0226801341646302 0.18978686137453 698 Protein containing GATA family zinc finger motifs transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YJR112W NNF1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0226641519120941 0.189437540267807 699 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore YJL157C FAR1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEUS|SITE OF POLARIZED GROWTH 0.02264704648169 0.18977190352414 700 Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress maintenance of protein location in nucleus|pheromone-dependent signal transduction involved in conjugation with cellular fusion|cell cycle arrest in response to pheromone cyclin-dependent protein kinase inhibitor activity nucleus|mating projection tip|membrane|cytoplasm YBR097W VPS15 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|PROTEIN PHOSPHORYLATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0226247145492351 0.190209084100923 701 Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery protein retention in Golgi apparatus|vacuole inheritance|peroxisome degradation|protein phosphorylation|macroautophagy|inositol lipid-mediated signaling|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding|protein serine/threonine kinase activity mitochondrion|phosphatidylinositol 3-kinase complex I|phosphatidylinositol 3-kinase complex II|Golgi membrane YHR017W YSC83 hom FT MITOCHONDRION 0.0226112864911366 0.19067086698175 702 Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 biological_process molecular_function mitochondrial outer membrane|mitochondrion YER109C FLO8 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0226088039497661 0.190521009583436 703 Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YDL184C RPL41A hom FT TRANSLATION|RIBOSOME 0.0225838732008486 0.191010530926665 704 Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YPL225W YPL225W hom 0.0224395366061874 0.193862810950505 705 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|cytoplasm YNL013C_d YNL013C hom 0.0224298192315305 0.194122487502655 706 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Unknown Unknown Unknown YER044C-A MEI4 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.022407811991257 0.194493899379791 707 Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic DNA double-strand break formation molecular_function condensed nuclear chromosome YDL134C PPH21 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0224008643327285 0.194898829449532 708 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication actin filament organization|budding cell bud growth|protein dephosphorylation|G1/S transition of mitotic cell cycle|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity protein phosphatase type 2A complex YJR031C GEA1 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0223513000121934 0.195621805832984 709 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|Golgi-associated vesicle|extrinsic to membrane YNL095C_p YNL095C hom 0.0223399376850562 0.19584916078943 710 Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication biological_process molecular_function integral to membrane YER081W SER3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0223330501086335 0.196254140717365 711 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication serine family amino acid biosynthetic process phosphoglycerate dehydrogenase activity cytoplasm YOL122C SMF1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0221569867762599 0.199536612442153 712 Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins copper ion transport|cellular copper ion homeostasis|manganese ion transport|cadmium ion transport|cellular manganese ion homeostasis|cellular cadmium ion homeostasis|iron ion transport solute:hydrogen symporter activity|inorganic cation transmembrane transporter activity integral to membrane|plasma membrane YFR056C_d YFR056C hom 0.0220946033193844 0.200805495598299 713 Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Unknown Unknown Unknown YDR282C_p YDR282C hom 0.0220799267695898 0.201104870819784 714 Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation biological_process molecular_function cellular_component YIL039W TED1 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0220772478179983 0.201159551722268 715 Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum YOR080W DIA2 hom FT CELL CYCLE|PROTEOLYSIS|NUCLEUS|CHROMOSOME|UBIQUITIN LIGASE COMPLEX 0.0220758031522455 0.201189043737083 716 Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|regulation of DNA replication|invasive growth in response to glucose limitation|protein ubiquitination ubiquitin-protein ligase activity|DNA replication origin binding SCF ubiquitin ligase complex|nucleus|nuclear replication fork YOR166C SWT1 hom FT NUCLEUS 0.0220458660807306 0.201800901470729 717 RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain nuclear mRNA surveillance of mRNP export|transcription, DNA-dependent endoribonuclease activity nucleus|cytoplasm YHR101C BIG1 het FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0220112781901915 0.202509500362526 718 Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan fungal-type cell wall biogenesis molecular_function integral to endoplasmic reticulum membrane YBR107C IML3 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0219939370319118 0.202865448326319 719 Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 establishment of meiotic sister chromatid cohesion|ascospore formation|protein localization to chromosome, centromeric region|meiotic sister chromatid segregation|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome outer kinetochore YDR078C SHU2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0219320937599262 0.204814828261818 720 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function recombinational repair|maintenance of rDNA|error-free translesion synthesis molecular_function Shu complex YLL040C VPS13 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|MITOCHONDRION|GOLGI APPARATUS 0.0219301517124923 0.204178637211119 721 Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively protein retention in Golgi apparatus|late endosome to vacuole transport|protein targeting to vacuole|ascospore-type prospore membrane assembly molecular_function endosome|mitochondrion|prospore membrane|extrinsic to membrane YOR283W YOR283W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0219085025365469 0.204625744697415 722 Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene dephosphorylation phosphatase activity nucleus|cytoplasm YOR306C MCH5 hom FT PLASMA MEMBRANE 0.0218745445987164 0.205328490771001 723 Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport riboflavin transport monocarboxylic acid transmembrane transporter activity|riboflavin transporter activity integral to membrane|plasma membrane YLR361C DCR2 hom FT CELL CYCLE|SIGNALING 0.0218595629902738 0.205706729750236 724 Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START endoplasmic reticulum unfolded protein response|traversing start control point of mitotic cell cycle|protein dephosphorylation hydrolase activity, acting on ester bonds|phosphoprotein phosphatase activity|phosphoric ester hydrolase activity cellular_component YJL183W MNN11 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0218158894462853 0.206749715883418 725 Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p protein glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YGL031C RPL24A hom FT TRANSLATION|RIBOSOME 0.0218004823802847 0.206867257882459 726 Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDR404C RPB7 het FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|TRANSLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0217866836472717 0.207290455375847 727 RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation transcription initiation from RNA polymerase II promoter|positive regulation of translational initiation|positive regulation of nuclear-transcribed mRNA poly(A) tail shortening|nuclear-transcribed mRNA catabolic process, exonucleolytic|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|translation initiation factor binding|single-stranded RNA binding|single-stranded DNA binding nucleus|DNA-directed RNA polymerase II, core complex|cytoplasmic mRNA processing body|cytoplasm YCL027W FUS1 hom FT PROTEIN LOCALIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0216970962374254 0.209301170396897 728 Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate regulation of termination of mating projection growth|cytogamy|cortical protein anchoring molecular_function integral to plasma membrane|mating projection tip YKL189W HYM1 het FT CELL DIVISION|SITE OF POLARIZED GROWTH 0.0216969647271199 0.209032005820094 729 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response cytokinesis, completion of separation|regulation of cell shape|regulation of transcription, DNA-dependent|budding cell apical bud growth molecular_function incipient cellular bud site|intracellular|cellular bud|mating projection tip YDR258C HSP78 hom FT PROTEIN LOCALIZATION|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0216675072529508 0.209651004385796 730 Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates positive regulation of mitochondrial translation in response to stress|protein refolding|protein stabilization|protein import into mitochondrial matrix|mitochondrial genome maintenance|protein unfolding|cellular response to heat misfolded protein binding|ATPase activity mitochondrion|mitochondrial matrix YOR325W_d YOR325W hom 0.0216593031241895 0.209823636114488 731 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Unknown Unknown Unknown YDR473C PRP3 het FT RNA PROCESSING|NUCLEUS 0.021638822824135 0.21080013972226 732 Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YNR039C ZRG17 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0216367283172173 0.210299187344963 733 Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency zinc ion transport zinc ion transmembrane transporter activity|metal ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YGL001C ERG26 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0216251359741081 0.210543689432749 734 C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis ergosterol biosynthetic process C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity endoplasmic reticulum|endoplasmic reticulum membrane YOR278W HEM4 het FT COFACTOR METABOLISM 0.0216107855973442 0.210846647788658 735 Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria heme biosynthetic process uroporphyrinogen-III synthase activity cellular_component YJR067C YAE1 het FT NUCLEUS 0.0215923073697653 0.21123721542237 736 Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species biological_process molecular_function nucleus|cytoplasm YOL100W PKH2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0215793371108848 0.211511675262736 737 Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p regulation of nuclear-transcribed mRNA poly(A) tail shortening|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cell cortex|eisosome|nucleus YIL158W AIM20 hom FT CELL CYCLE 0.0215645593342131 0.211824697389584 738 Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process molecular_function fungal-type vacuole YOR303W CPA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0215601816653156 0.211917489170589 739 Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader arginine biosynthetic process carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity carbamoyl-phosphate synthase complex YNR062C_p YNR062C hom 0.0215352086985183 0.212447393165284 740 Putative membrane protein of unknown function biological_process molecular_function integral to membrane YOR040W GLO4 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0215343517507983 0.212465593790371 741 Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate cellular carbohydrate metabolic process|methylglyoxal catabolic process to D-lactate hydroxyacylglutathione hydrolase activity mitochondrion|mitochondrial matrix YER140W EMP65 hom FT MITOCHONDRION 0.0215188615344309 0.212794783023382 742 ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies protein folding in endoplasmic reticulum molecular_function mitochondrion|integral to membrane YGL122C NAB2 het FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.021436320271304 0.214555110792551 743 Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress poly(A)+ mRNA export from nucleus|mRNA polyadenylation|regulation of mRNA stability poly(A) RNA binding nucleus|cytoplasm YNL222W SSU72 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0214239262443229 0.214820337303915 744 Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo transcription initiation from RNA polymerase II promoter|mRNA 3'-end processing|mRNA cleavage|termination of RNA polymerase II transcription|termination of RNA polymerase II transcription, poly(A)-coupled|transcription antitermination|transcriptional start site selection at RNA polymerase II promoter|dephosphorylation of RNA polymerase II C-terminal domain|termination of RNA polymerase II transcription, exosome-dependent|snoRNA transcription|transcription elongation from RNA polymerase II promoter protein tyrosine phosphatase activity|phosphoprotein phosphatase activity|CTD phosphatase activity mRNA cleavage and polyadenylation specificity factor complex|nucleus YKL213C DOA1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0214142516564082 0.215919289755603 745 WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress double-strand break repair via nonhomologous end joining|ubiquitin-dependent protein catabolic process ubiquitin binding nucleus|cytoplasm YAL021C CCR4 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS|RNA POL II, HOLOENZYME 0.0214058327870583 0.215207953026553 746 Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|DNA replication checkpoint|nuclear-transcribed mRNA poly(A) tail shortening|traversing start control point of mitotic cell cycle|replication fork protection|transcription elongation from RNA polymerase II promoter|DNA replication 3'-5'-exoribonuclease activity CCR4-NOT core complex|cytoplasmic mRNA processing body|Cdc73/Paf1 complex|cytoplasm YOL003C PFA4 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0213983018790273 0.215369435875821 747 Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein palmitoylation palmitoyltransferase activity integral to membrane|endoplasmic reticulum YOR218C_d YOR218C het 0.0213935291926247 0.215471820224103 748 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Unknown Unknown Unknown YDL118W_d YDL118W hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0213651166384748 0.216082055787805 749 Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component YDR245W MNN10 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0213566284159715 0.216470299502826 750 Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family N-glycan processing|cell wall mannoprotein biosynthetic process|substituted mannan metabolic process|barrier septum assembly alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YKR030W GMH1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0213476941031275 0.216456865950217 751 Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting transport molecular_function integral to membrane|integral to Golgi membrane YDR206W EBS1 hom FT TRANSLATION|NUCLEUS|CHROMOSOME 0.0212551070402275 0.218525245208836 752 Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors negative regulation of translation|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay molecular_function cytoplasmic mRNA processing body|cytoplasm YBR133C HSL7 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0212273083695221 0.219059504878033 753 Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress G2/M transition of mitotic cell cycle|regulation of cell cycle protein-arginine omega-N symmetric methyltransferase activity|protein-arginine omega-N monomethyltransferase activity cellular bud neck YDL058W USO1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|CYTOSKELETON 0.0212140128550052 0.219692461236332 754 Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Golgi vesicle docking|ER to Golgi vesicle-mediated transport|SNARE complex assembly molecular_function integral to membrane|ER to Golgi transport vesicle membrane|Golgi membrane YJR011C_p YJR011C hom 0.0211947887068561 0.219766390472724 755 Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YJL099W CHS6 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0211773325538432 0.220353103612258 756 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YOR249C APC5 het FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0211756482969275 0.220183213105261 757 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YDL009C_p YDL009C hom 0.0211678532183253 0.220353129664473 758 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene biological_process molecular_function cellular_component YOR013W_d IRC11 hom 0.021164778102013 0.22062683965488 759 Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YJL028W YJL028W hom 0.0211502045491091 0.220738181413567 760 Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome YGR142W BTN2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0211481963435412 0.221195725572995 761 v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication intracellular protein transport|amino acid transport|retrograde transport, endosome to Golgi|regulation of pH SNARE binding cytosol|late endosome|perinuclear region of cytoplasm YEL045C_d YEL045C hom 0.0211212471382069 0.221371007200628 762 Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Unknown Unknown Unknown YNR044W AGA1 hom 0.021109362729993 0.221631100941185 763 Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds agglutination involved in conjugation with cellular fusion cell adhesion molecule binding fungal-type cell wall YMR203W TOM40 het FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0210953602237823 0.222352041855734 764 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore protein import into mitochondrial matrix protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YCR021C HSP30 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0210654242547368 0.222594607420968 765 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase negative regulation of ATPase activity|response to stress molecular_function integral to membrane|plasma membrane YJL054W TIM54 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0210578372598844 0.222761282110287 766 Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane protein import into mitochondrial inner membrane protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YER151C UBP3 hom FT VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS 0.021056161696408 0.222798103714767 767 Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress protein deubiquitination|ribophagy|regulation of ER to Golgi vesicle-mediated transport|regulation of response to osmotic stress ubiquitin-specific protease activity|mRNA binding cytoplasm YCR079W PTC6 hom FT RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0210097692139369 0.223819338736684 768 Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase mitochondrion degradation|positive regulation of catalytic activity|macroautophagy|protein dephosphorylation protein serine/threonine phosphatase activity mitochondrion|mitochondrial matrix|mitochondrial intermembrane space YMR048W CSM3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0210082559746472 0.223852705840414 769 Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress DNA repair|DNA replication checkpoint|meiotic chromosome segregation|maintenance of DNA repeat elements|replication fork arrest|replication fork protection|mitotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion molecular_function replication fork protection complex|nucleus YNR049C MSO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0209733898778257 0.224968570379269 770 Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress cellular membrane fusion|ascospore-type prospore membrane assembly|vesicle docking involved in exocytosis molecular_function SNARE complex|cellular bud membrane|plasma membrane|prospore membrane|cellular bud neck|cellular bud tip YER152C YER152C hom FT NUCLEUS 0.0209517736444232 0.225100691851477 771 Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene biological_process 2-aminoadipate transaminase activity nucleus|cytoplasm YDR471W RPL27B hom FT TRANSLATION|RIBOSOME 0.0209243239159791 0.225708988364278 772 Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YNL133C FYV6 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0208983676515016 0.226285267292325 773 Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining double-strand break repair via nonhomologous end joining molecular_function nucleus YCR008W SAT4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0208675481706013 0.226970880742952 774 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p cellular cation homeostasis|protein phosphorylation|cellular protein localization|G1/S transition of mitotic cell cycle|protein dephosphorylation protein kinase activity cellular_component YGR059W SPR3 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.02085742722365 0.227196355035763 775 Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI ascospore formation structural molecule activity septin complex|ascospore wall|prospore membrane YLR132C USB1 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0208397275713695 0.227591051177655 776 Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants U6 snRNA 3'-end processing phosphoric diester hydrolase activity mitochondrion|nucleus|cytoplasm YLR280C_d YLR280C hom 0.0207178036396687 0.230323187866847 777 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR063C LAS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.020709453176019 0.23058072378308 778 Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability maturation of LSU-rRNA|maturation of 5.8S rRNA molecular_function mitochondrion|nucleus|preribosome|cytoplasm YEL011W GLC3 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0206990557038856 0.230745360572177 779 Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress glycogen biosynthetic process 1,4-alpha-glucan branching enzyme activity cytoplasm YCL017C NFS1 het FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|COFACTOR METABOLISM|NUCLEUS|MITOCHONDRION 0.0206831228309628 0.231104574550205 780 Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria cellular iron ion homeostasis|iron-sulfur cluster assembly|mitochondrial tRNA thio-modification|tRNA thio-modification|tRNA wobble uridine modification cysteine desulfurase activity mitochondrion|nucleus YJR154W_p YJR154W hom 0.02062072917171 0.23251509054031 781 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YNL200C YNL200C hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0206033614868464 0.23290880105152 782 NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies nicotinamide nucleotide metabolic process NADHX epimerase activity mitochondrion|cytoplasm YGL101W_p YGL101W hom FT NUCLEUS 0.0205837428742028 0.233354106425758 783 Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p biological_process molecular_function nucleus|cytoplasm YOL159C YOL159C hom 0.0205494244345896 0.234134520372234 784 Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA biological_process molecular_function cellular_component YPR101W SNT309 hom FT RNA PROCESSING|NUCLEUS 0.0205215252899805 0.234770317986692 785 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YMR244W_p YMR244W hom 0.0205167101044064 0.234880175490633 786 Putative protein of unknown function biological_process molecular_function cellular_component YKR024C DBP7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0205045645032586 0.235506980376285 787 Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YOL043C NTG2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0205025032565281 0.235204513927012 788 DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication base-excision repair|base-excision repair, AP site formation oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity|DNA-(apurinic or apyrimidinic site) lyase activity nucleus YKL091C YKL091C hom FT NUCLEUS 0.0204993755376099 0.235275961433926 789 Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study biological_process phosphatidylcholine binding|phosphatidylinositol transporter activity|phosphatidylinositol binding|phosphatidylcholine transporter activity nucleus YJL208C NUC1 hom FT NUCLEUS|MITOCHONDRION 0.0204585518411844 0.236209918929293 790 Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy DNA catabolic process|RNA catabolic process|apoptotic process|DNA recombination ribonuclease activity|endodeoxyribonuclease activity|exodeoxyribonuclease activity mitochondrion|mitochondrial inner membrane|nucleus YJR083C ACF4 hom 0.0204388151913945 0.23666238923702 791 Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate biological_process molecular_function cellular_component YGR237C_p YGR237C hom 0.0203568644221003 0.238828083825431 792 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YGR161W-C_p YGR161W-C hom 0.0203551029328984 0.238588332065232 793 Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function cellular_component YIR019C FLO11 hom FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.020323626733667 0.239315347937434 794 GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain cell-cell adhesion|biofilm formation|pseudohyphal growth|filamentous growth|flocculation|invasive growth in response to glucose limitation molecular_function extracellular region|plasma membrane|cellular bud neck YDR421W ARO80 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0203213477582496 0.239368046728969 795 Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids aromatic amino acid family catabolic process|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|nucleic acid binding transcription factor activity nucleus YOR302W YOR302W hom FT TRANSLATION 0.0202702049859171 0.240552819070241 796 CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation translation regulator activity cytosol YGL132W_d YGL132W hom 0.0202408789195559 0.241234045936456 797 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Unknown Unknown Unknown YDR317W HIM1 hom FT RESPONSE TO DNA DAMAGE 0.0202275761263086 0.241543509008838 798 Protein of unknown function involved in DNA repair DNA repair molecular_function cellular_component YDR457W TOM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.0202092739673764 0.241969728717062 799 E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mitosis|nucleocytoplasmic transport|nucleus organization|cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of cell size|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|nucleolus|nucleus YOR319W HSH49 het FF|FT RNA PROCESSING|NUCLEUS 0.0202059884640856 0.242046297308072 800 U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YKL032C IXR1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0201950081759741 0.242442885317942 801 Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b DNA repair|cellular response to hypoxia|negative regulation of transcription from RNA polymerase II promoter damaged DNA binding|sequence-specific DNA binding nuclear chromatin YEL067C_p YEL067C hom FT MITOCHONDRION 0.0201944411267062 0.242315542958908 802 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YNL144C_p YNL144C hom FT MITOCHONDRION 0.0201804828894084 0.242641283825262 803 Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study biological_process molecular_function mitochondrion YIL152W_p YIL152W hom 0.0201588232576524 0.243147359873699 804 Putative protein of unknown function biological_process molecular_function cellular_component YJL173C RFA3 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0201317083098826 0.243781943679493 805 Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YBR245C ISW1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0201314300811367 0.24378846121875 806 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions termination of RNA polymerase I transcription|nucleosome positioning|termination of RNA polymerase II transcription|regulation of transcription by chromatin organization|DNA-dependent transcription, elongation|regulation of transcriptional start site selection at RNA polymerase II promoter|heterochromatin maintenance involved in chromatin silencing|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter nucleosome binding|ATPase activity|rDNA binding|DNA binding ISW1 complex|nucleolar chromatin YBR258C SHG1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0201311303530284 0.243795482519847 807 Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres histone H3-K4 methylation histone methyltransferase activity (H3-K4 specific) nucleus|Set1C/COMPASS complex YGR153W_p YGR153W hom 0.0201109337845574 0.244339303848366 808 Putative protein of unknown function biological_process molecular_function cellular_component YBR277C_d YBR277C hom 0.020083617495247 0.244910293862272 809 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Unknown Unknown Unknown YFL018C LPD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0200800407148749 0.244994361639547 810 Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes L-serine biosynthetic process|2-oxoglutarate metabolic process|leucine catabolic process|glycine catabolic process|isoleucine catabolic process|pyruvate metabolic process|valine catabolic process|hydrogen peroxide metabolic process glycine dehydrogenase (decarboxylating) activity|pyruvate dehydrogenase activity|oxoglutarate dehydrogenase (succinyl-transferring) activity|dihydrolipoyl dehydrogenase activity mitochondrial nucleoid|mitochondrion|glycine cleavage complex|mitochondrial oxoglutarate dehydrogenase complex YCR009C RVS161 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0200787496668679 0.245165571687476 811 Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress conjugation with cellular fusion|actin cytoskeleton reorganization|endocytosis|actin cortical patch localization|response to starvation|response to osmotic stress|lipid tube assembly|actin cytoskeleton organization cytoskeletal protein binding|lipid binding membrane raft|mating projection tip|mating projection|actin cortical patch YHL001W RPL14B hom FT TRANSLATION|RIBOSOME 0.0200723976106078 0.245455922457526 812 Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YKR061W KTR2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0200701583860817 0.245226738700484 813 Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication cell wall mannoprotein biosynthetic process|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YDR009W GAL3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0200171799765673 0.246757249723316 814 Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication maintenance of protein location|galactose metabolic process|positive regulation of transcription from RNA polymerase II promoter by galactose protein binding nucleus|cytoplasm YHR079C-A SAE3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0199744251872157 0.247485850174505 815 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p double-strand break repair|reciprocal meiotic recombination|meiotic DNA recombinase assembly molecular_function condensed nuclear chromosome YPL063W TIM50 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0199719707148413 0.247543961660094 816 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel protein import into mitochondrial matrix mitochondrion targeting sequence binding|protein channel activity mitochondrion|mitochondrial inner membrane presequence translocase complex YDL173W PAR32 hom 0.0199514552628936 0.248030053916281 817 Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene biological_process molecular_function cytoplasm YNR023W SNF12 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RSC COMPLEX 0.0199377962969281 0.24870724334761 818 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YOR174W MED4 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0199254520205182 0.248647132532113 819 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter molecular_function core mediator complex YLR018C POM34 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0198991439889097 0.249343168336151 820 Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication nuclear pore organization|protein import into nucleus|spindle pole body duplication associated with nuclear envelope structural constituent of nuclear pore nuclear pore transmembrane ring|integral to membrane|integral to nuclear outer membrane|nuclear pore YDR270W CCC2 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0198966028664651 0.249333001938419 821 Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism cellular iron ion homeostasis|copper ion export|transmembrane transport copper ion binding|cation-transporting ATPase activity integral to membrane|trans-Golgi network transport vesicle membrane YHL034C SBP1 hom FT TRANSLATION|NUCLEUS 0.0198937214712514 0.249401577717657 822 Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 negative regulation of translation|negative regulation of translation in response to stress RNA binding|eukaryotic initiation factor 4G binding nucleolus|cytoplasmic mRNA processing body|cytoplasm YCR044C PER1 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0198524943925513 0.250525602748918 823 Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 GPI anchor biosynthetic process|cellular manganese ion homeostasis molecular_function fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum YDR242W AMD2 hom 0.0198185073850725 0.252117637422498 824 Putative amidase biological_process amidase activity cellular_component YOR315W SFG1 hom FT CELL CYCLE|NUCLEUS 0.0198124000529093 0.251342422565616 825 Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate mitotic cell cycle|pseudohyphal growth molecular_function nucleus|cytoplasm YER049W TPA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0198028847853551 0.252278733516473 826 Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|translational termination poly(A) RNA binding nucleus YKL070W_p YKL070W hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0197857909370075 0.252262960556469 827 Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOR219C STE13 hom FT PROTEOLYSIS|GOLGI APPARATUS 0.0197591461374617 0.25261909166397 828 Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor peptide pheromone maturation aminopeptidase activity trans-Golgi network YKL162C-A_d YKL162C-A hom 0.0197426326324749 0.253015889858063 829 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL165C_p YIL165C hom 0.0197020103248562 0.253993837658528 830 Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene mitochondrion degradation molecular_function cellular_component YDR499W LCD1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0196825868627796 0.25446236921824 831 Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress establishment of protein localization|telomere maintenance via telomerase|DNA damage checkpoint damaged DNA binding nuclear chromosome YGL073W HSF1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|CYTOSKELETON ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0196545683356542 0.255139288645591 832 Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated spindle pole body duplication|negative regulation of TOR signaling cascade|response to heat|regulation of transcription from RNA polymerase II promoter|response to stress sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity mitochondrion|nucleus YOL037C_d YOL037C hom 0.0196493077713614 0.255337432409188 833 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Unknown Unknown Unknown YGL169W SUA5 het FT RNA PROCESSING|TRANSLATION|NUCLEUS|CHROMOSOME 0.0196103034567317 0.256566264838547 834 Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family threonylcarbamoyladenosine metabolic process|telomere maintenance|regulation of translational fidelity single-stranded telomeric DNA binding cytoplasm YJL078C PRY3 hom 0.0196102250776201 0.256213164505129 835 Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p biological_process molecular_function fungal-type cell wall YDL083C RPS16B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0195713850937626 0.257511543130104 836 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YMR302C YME2 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0195619988228044 0.257739884917016 837 Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases mitochondrial genome maintenance molecular_function mitochondrion|integral to mitochondrial inner membrane YGL170C SPO74 hom FT NUCLEUS|CYTOSKELETON 0.0195601761974992 0.257499984021908 838 Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation ascospore formation structural molecule activity spindle pole body YGR236C SPG1 hom FT MITOCHONDRION 0.0195571519767041 0.257502574444372 839 Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDR138W HPR1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0194967558957928 0.258975361918906 840 Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|response to DNA damage stimulus|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|Cdc73/Paf1 complex|nucleoplasmic THO complex YDR102C_d YDR102C hom 0.0194494756615892 0.260132376696748 841 Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Unknown Unknown Unknown YNL100W AIM37 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0194236103159302 0.260766849654591 842 Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YMR261C TPS3 hom FT CARBOHYDRATE METABOLISM 0.0194195826448521 0.260865744002183 843 Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication trehalose biosynthetic process|response to stress enzyme regulator activity|alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity|trehalose-phosphatase activity alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) YMR124W_p YMR124W hom 0.0193833644905258 0.261969807132316 844 Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization biological_process molecular_function cytoplasm YNL038W GPI15 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0193616898074048 0.262361293317304 845 Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YDL104C QRI7 hom FT RNA PROCESSING|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0193446368866119 0.262710672968538 846 Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification mitochondrial tRNA threonylcarbamoyladenosine modification molecular_function mitochondrion YCR067C SED4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.019335755058334 0.263214948095995 847 Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p positive regulation of GTPase activity|regulation of COPII vesicle coating Sar GTPase activator activity integral to endoplasmic reticulum membrane YKL130C SHE2 hom FT RNA LOCALIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0193030665440712 0.263737877872841 848 RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud mating type switching|intracellular mRNA localization mRNA binding cellular bud tip|cytoplasm YOR008C SLG1 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.019290800191047 0.264255374600585 849 Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response positive regulation of endocytosis|Rho protein signal transduction|actin cytoskeleton organization|fungal-type cell wall organization|response to heat|peroxisome degradation|establishment of cell polarity|response to osmotic stress transmembrane signaling receptor activity plasma membrane|mating projection tip|cellular bud neck YNL187W SWT21 hom FT RNA PROCESSING|NUCLEUS 0.0192881893248588 0.264106166595389 850 Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats mRNA splicing, via spliceosome molecular_function small nuclear ribonucleoprotein complex|nucleus YGL079W_p KXD1 hom 0.0192855196020954 0.264172293645931 851 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome biological_process molecular_function endosome YBR030W RKM3 hom FT NUCLEUS 0.0192686865918367 0.264589497621046 852 Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity nucleus YGR225W AMA1 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0192574204488039 0.26508293783892 853 Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis ascospore wall assembly|positive regulation of protein catabolic process|ascospore formation|meiosis I enzyme activator activity anaphase-promoting complex YOL036W_p YOL036W hom 0.0192265709152076 0.265635317194464 854 Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication biological_process molecular_function cellular_component YKL003C MRP17 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0192212018912329 0.265768845395119 855 Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YMR039C SUB1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0191545111401838 0.267431302529249 856 Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress termination of RNA polymerase II transcription|hyperosmotic response|RNA polymerase III transcriptional preinitiation complex assembly|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase III promoter transcription coactivator activity nucleus YPL096C-A ERI1 hom FT LIPID METABOLISM|SIGNALING|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.019146617585355 0.267842791600659 857 Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein GPI anchor biosynthetic process|Ras protein signal transduction GTPase inhibitor activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|endoplasmic reticulum membrane YNL290W RFC3 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0191111510029054 0.269088044911974 858 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity|ATPase activity Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|nucleus|Ctf18 RFC-like complex YNL188W KAR1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.019107345040535 0.269111833024725 859 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p karyogamy involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope protein binding half bridge of spindle pole body YBL050W SEC17 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION 0.0191066103386029 0.268629767302813 860 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP autophagy|SNARE complex disassembly|vesicle fusion with Golgi apparatus|vacuole fusion, non-autophagic ATPase activator activity|soluble NSF attachment protein activity SNARE complex|cytosol|extrinsic to membrane YDR348C PAL1 hom FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.01909086678579 0.269024470365713 861 Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cell cortex|cellular bud neck|mating projection tip YIR018W YAP5 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0190853755716802 0.26916223291522 862 Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication negative regulation of chromatin silencing at telomere|negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to iron|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity nuclear chromatin|nucleus YDR246W-A_p YDR246W-A hom 0.0190800216589024 0.26929659746743 863 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHR137W ARO9 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0190682098342776 0.269664653555526 864 Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism aromatic amino acid family metabolic process aromatic-amino-acid:2-oxoglutarate aminotransferase activity nucleus|cytoplasm YMR305C SCW10 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM 0.0190552403174286 0.269919122112359 865 Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p conjugation with cellular fusion glucosidase activity fungal-type cell wall|extracellular region|endoplasmic reticulum|cytoplasm YEL057C_p YEL057C hom 0.0190326511544093 0.270558923256007 866 Protein of unknown function involved in telomere maintenance; target of UME6 regulation biological_process molecular_function cellular_component YLR310C CDC25 het FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEUS|PLASMA MEMBRANE 0.0190170175297088 0.271167376681961 867 Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 regulation of cell cycle|replicative cell aging|Ras protein signal transduction|traversing start control point of mitotic cell cycle Ras guanyl-nucleotide exchange factor activity cytosol|nucleus|plasma membrane|cytoplasm YNL050C_p YNL050C hom 0.0189954055969999 0.271426276845937 868 Putative protein of unknown function; YNL050c is not an essential gene biological_process molecular_function cellular_component YMR236W TAF9 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.0189731207738536 0.272060604539833 869 Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YER042W MXR1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0189411308539389 0.27315638541887 870 Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan cellular response to oxidative stress peptide-methionine-(S)-S-oxide reductase activity nucleus|cytoplasm YBR090C_p YBR090C hom FT NUCLEUS 0.0189363150007707 0.273421761737448 871 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YPR190C RPC82 het FT NUCLEUS 0.0189363098255283 0.272992026037222 872 RNA polymerase III subunit C82 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YAL020C ATS1 hom FT RNA PROCESSING|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0189210400486476 0.273307460025838 873 Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle microtubule-based process|budding cell bud growth|microtubule cytoskeleton organization|tRNA wobble uridine modification molecular_function cytoplasm YMR116C ASC1 hom FT SIGNALING|TRANSLATION|RIBOSOME 0.0187580585606106 0.277461417039509 874 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation negative regulation of translation|glucose mediated signaling pathway|G-protein coupled receptor signaling pathway|invasive growth in response to glucose limitation G-protein alpha-subunit binding|GDP-dissociation inhibitor activity|signal transducer activity cytosolic small ribosomal subunit|cytoplasm YKR060W UTP30 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS 0.0187025055723282 0.278887084352764 875 Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit ribosomal small subunit biogenesis molecular_function nucleolus|nucleus|90S preribosome YDR154C_d YDR154C hom 0.018678159210521 0.279585201407012 876 Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YER002W NOP16 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0186320828955471 0.280773276939692 877 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YGL252C RTG2 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|SIGNALING|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0186254090752286 0.280873866504066 878 Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p maintenance of DNA trinucleotide repeats|regulation of transcription from RNA polymerase II promoter|Unknown|nitrogen catabolite regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|mitochondria-nucleus signaling pathway|transcription factor import into nucleus RNA polymerase II transcription factor binding transcription factor activity SLIK (SAGA-like) complex|transcriptionally active chromatin|cytoplasm YIR003W AIM21 hom FT SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0186201813463693 0.281008932326495 879 Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton mitochondrion migration along actin filament molecular_function ribosome|mating projection tip|actin cytoskeleton YMR322C_p SNO4 hom FT PROTEOLYSIS 0.0186163207441936 0.281108704770669 880 Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation biological_process cysteine-type peptidase activity cellular_component YGL100W SEH1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE|NUCLEAR PORE 0.0185746213328477 0.282403403818482 881 Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 nucleocytoplasmic transport structural constituent of nuclear pore fungal-type vacuole membrane|integral to membrane|nuclear pore|Seh1-associated complex|nuclear pore outer ring YKL157W APE2 hom FT PROTEOLYSIS|MITOCHONDRION 0.0185577367971618 0.28262568365745 882 Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication peptide catabolic process metalloaminopeptidase activity mitochondrion|extracellular region|cell wall-bounded periplasmic space YKL054C DEF1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|CHROMOSOME 0.0185147552392289 0.284389608533682 883 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis transcription-coupled nucleotide-excision repair|telomere maintenance|response to DNA damage stimulus|ubiquitin-dependent protein catabolic process molecular_function nucleus YLR152C_p YLR152C hom 0.0185104986015403 0.284284336657854 884 Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function integral to membrane YHR156C LIN1 hom FT NUCLEUS|CHROMOSOME 0.0184834285091606 0.284557796217254 885 Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication biological_process protein binding chromatin|nucleus|U4/U6 x U5 tri-snRNP complex|U5 snRNP YNL093W YPT53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.0184563631719973 0.285263744583786 886 Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication endocytosis|protein targeting to vacuole GTPase activity late endosome YML088W UFO1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0184407745275826 0.285670881794723 887 F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury|response to DNA damage stimulus ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex|nucleus|cytoplasm YOR104W PIN2 hom 0.0184388012470717 0.285722447027345 888 Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated biological_process molecular_function cellular_component YGL216W KIP3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0183964885802255 0.286829663300682 889 Kinesin-related motor protein involved in mitotic spindle positioning nuclear migration along microtubule|mitotic spindle disassembly|establishment of mitotic spindle orientation|plus-end specific microtubule depolymerization|mitotic spindle organization in nucleus plus-end-directed microtubule motor activity|tubulin-dependent ATPase activity kinesin complex|cytoplasmic microtubule|nuclear microtubule YPR058W YMC1 hom FT MITOCHONDRION 0.0183408406903408 0.288794282423521 890 Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication mitochondrial transport organic acid transmembrane transporter activity mitochondrion|integral to membrane YAL030W SNC1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0183328894071594 0.288859276078287 891 Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication endocytosis|exocytosis|Golgi to plasma membrane transport|vesicle fusion SNAP receptor activity SNARE complex|trans-Golgi network|endosome|transport vesicle membrane|plasma membrane|cellular bud neck YML126C ERG13 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0183116291471116 0.289058938519861 892 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis ergosterol biosynthetic process hydroxymethylglutaryl-CoA synthase activity endoplasmic reticulum YOL082W ATG19 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.018291153230738 0.290102807295029 893 Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles vesicle organization|ER-associated protein catabolic process|protein processing|protein complex localization|CVT pathway protein binding, bridging pre-autophagosomal structure|cytoplasm|CVT complex|extrinsic to membrane YJL137C GLG2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0182794803550788 0.29033868626513 894 Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication glycogen biosynthetic process glycogenin glucosyltransferase activity cytoplasm YOL164W-A_p YOL164W-A hom 0.0182771845977822 0.290039094199436 895 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHR167W THP2 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0182697233760295 0.290164104506986 896 Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance mRNA export from nucleus|transcription elongation from RNA polymerase II promoter|DNA recombination nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YIL121W QDR2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0182549898135353 0.290553342040013 897 Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper drug transmembrane transport|potassium ion import drug transmembrane transporter activity integral to membrane|integral to plasma membrane YBR283C SSH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0182535589073044 0.290591163029025 898 Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential SRP-dependent cotranslational protein targeting to membrane protein transmembrane transporter activity|signal sequence binding integral to membrane|Ssh1 translocon complex YIL119C RPI1 hom FT SIGNALING|NUCLEUS 0.0182318904379541 0.291164297704092 899 Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF fungal-type cell wall biogenesis|Ras protein signal transduction|positive regulation of transcription from RNA polymerase II promoter small GTPase regulator activity nucleus YML038C YMD8 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0182255465280399 0.291332238874317 900 Putative nucleotide sugar transporter, has similarity to Vrg4p UDP-glucose transport|nucleotide-sugar transport nucleotide-sugar transmembrane transporter activity COPI-coated vesicle|integral to membrane YPL113C_p YPL113C hom FT OXIDATION-REDUCTION PROCESS 0.0182151290996845 0.291608158743037 901 Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene metabolic process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor cellular_component YDR407C TRS120 het FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.018213865285277 0.291641644537446 902 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic regulation of Rab GTPase activity|early endosome to Golgi transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity trans-Golgi network|TRAPP complex|early endosome YBR089C-A NHP6B hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0181742953995624 0.292691387649883 903 High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication RNA polymerase III transcriptional preinitiation complex assembly|chromatin remodeling|transcription from RNA polymerase II promoter nucleosome binding|DNA binding, bending|sequence-specific DNA binding nucleus YDR425W SNX41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT 0.0181715450728872 0.292980563055687 904 Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p retrograde transport, endosome to Golgi phosphatidylinositol binding|phosphatidylinositol-3-phosphate binding endosome YGR073C_d YGR073C het 0.0181686319356216 0.292841839961188 905 Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Unknown Unknown Unknown YNL147W LSM7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0181513069085287 0.29330240877835 906 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasm YPL217C BMS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|NUCLEUS|MITOCHONDRION 0.0181414428313257 0.293564852526177 907 GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p ribosome assembly|rRNA processing U3 snoRNA binding|GTP binding|GTPase activity mitochondrion|nucleolus|nucleus|90S preribosome|cytoplasm YHR142W CHS7 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.018127775002469 0.294361307361018 908 Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress protein folding|ER to Golgi vesicle-mediated transport|fungal-type cell wall chitin biosynthetic process unfolded protein binding integral to membrane|endoplasmic reticulum membrane YNL114C_d YNL114C het 0.0181059137067926 0.294511445583846 909 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Unknown Unknown Unknown YDR335W MSN5 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|NUCLEUS 0.0180907819709113 0.294915216670901 910 Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm tRNA re-export from nucleus|protein export from nucleus protein binding|importin-alpha export receptor activity integral to membrane|nucleus|cytoplasm YNL178W RPS3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0180900225147366 0.294935491556825 911 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome|DNA-(apurinic or apyrimidinic site) lyase activity preribosome, small subunit precursor|cytosolic small ribosomal subunit|90S preribosome YOL094C RFC4 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0180656597716176 0.295586388881199 912 Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity Elg1 RFC-like complex|Rad17 RFC-like complex|DNA replication factor C complex|Ctf18 RFC-like complex YIR004W DJP1 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0180560480486136 0.295843448739992 913 Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ protein import into peroxisome matrix chaperone binding cytosol YOR046C DBP5 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH|NUCLEAR PORE 0.0180477317063194 0.296065985077346 914 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p translational termination|mRNA export from nucleus RNA helicase activity|inositol hexakisphosphate binding|RNA-dependent ATPase activity nuclear pore cytoplasmic filaments|nucleus|polysome|nuclear pore|cellular bud tip|cytoplasm YLR364W GRX8 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0180477003891283 0.296066823303146 915 Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic biological_process glutathione-disulfide reductase activity cytoplasm YBR195C MSI1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0180242731407433 0.296694314282598 916 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase DNA replication-dependent nucleosome assembly histone binding nucleus|CAF-1 complex|cytoplasm YFR039C_p YFR039C hom 0.0180179843374443 0.296862909037202 917 Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component YJL199C_d MBB1 hom 0.0179803819263295 0.297872317564677 918 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Unknown Unknown Unknown YBR183W YPC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0179800875514268 0.29788022887001 919 Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication ceramide catabolic process|ceramide biosynthetic process phytoceramidase activity|sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YIL024C_p YIL024C hom 0.0179663307376366 0.298250098806229 920 Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p biological_process molecular_function cellular_component YLR194C YLR194C hom FT CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0179568484098273 0.298865931010032 921 Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall|anchored to plasma membrane YLL034C RIX7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0179389566883816 0.298986997609994 922 Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus ATPase activity nucleolus|nucleus|preribosome, large subunit precursor YFL002C SPB4 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0179375813150863 0.299024054145669 923 Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients ribosomal large subunit assembly|rRNA processing ATP-dependent RNA helicase activity nucleolus|90S preribosome YER016W BIM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0179103619716094 0.299758052325949 924 Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally nuclear migration along microtubule|microtubule nucleation|microtubule depolymerization|negative regulation of microtubule depolymerization|positive regulation of microtubule polymerization|mitotic sister chromatid cohesion|mitotic cell cycle spindle assembly checkpoint microtubule plus-end binding|protein homodimerization activity|structural constituent of cytoskeleton spindle midzone|microtubule plus end|cytoplasmic microtubule|spindle pole body|kinetochore|spindle pole|spindle YML091C RPM2 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0179044347901972 0.299918044109344 925 Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus cytoplasmic translation|tRNA 5'-leader removal|mRNA processing|mitochondrion organization|RNA processing|positive regulation of transcription from RNA polymerase II promoter ribonuclease P activity mitochondrion|nucleus|mitochondrial matrix|cytoplasmic mRNA processing body|mitochondrial ribonuclease P complex YOR199W_d YOR199W hom 0.0178974183171758 0.300468313052331 926 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR158C-A_p YMR158C-A hom 0.0178642682308486 0.301003755388366 927 Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene biological_process molecular_function cellular_component YJR005W APL1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE 0.0178571271730448 0.301197053456701 928 Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex vesicle-mediated transport molecular_function AP-2 adaptor complex YML086C ALO1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.017831597948918 0.30188876814615 929 D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress cellular response to oxidative stress|dehydro-D-arabinono-1,4-lactone biosynthetic process D-arabinono-1,4-lactone oxidase activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YOL153C YOL153C hom 0.0178299947856772 0.301932241115337 930 Hypothetical protein biological_process molecular_function cellular_component YJL182C_d YJL182C hom 0.0178080083430421 0.302528867561679 931 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Unknown Unknown Unknown YLR315W NKP2 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0178075485750445 0.303408502585696 932 Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 biological_process molecular_function spindle pole body|kinetochore YNL225C CNM67 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0177955452547127 0.302867415112485 933 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication microtubule nucleation|mitotic spindle organization structural constituent of cytoskeleton outer plaque of spindle pole body|spindle pole body YMR184W ADD37 hom FT PROTEOLYSIS 0.0177904548631844 0.303005762996126 934 Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress ER-associated protein catabolic process molecular_function cytoplasm YNL259C ATX1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS 0.0177860810778891 0.303341171531694 935 Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake copper ion transport|cellular response to oxidative stress|cellular iron ion homeostasis copper chaperone activity cytosol YEL012W UBC8 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS 0.0177810039542589 0.303262733422882 936 Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YMR118C_p SHH3 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0177647823423167 0.30370413686465 937 Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase biological_process molecular_function integral to membrane YDR305C HNT2 hom FT NUCLEUS|MITOCHONDRION 0.0177436303947269 0.304280339396453 938 Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins nucleoside catabolic process hydrolase activity|nucleoside-triphosphatase activity mitochondrion|nucleus|cytoplasm YGL213C SKI8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0177085792973595 0.305236766661937 939 Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|protein complex assembly|nuclear-transcribed mRNA catabolic process, non-stop decay|protein-DNA complex assembly|reciprocal meiotic recombination molecular_function Ski complex|nuclear chromosome YIL164C NIT1 hom 0.0177034539216879 0.305448952053763 940 Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene biological_process nitrilase activity cellular_component YIL094C LYS12 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0176929433825335 0.305664060820091 941 Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate lysine biosynthetic process homoisocitrate dehydrogenase activity mitochondrion YGR232W NAS6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0176927471401668 0.306464471111082 942 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 proteolysis|proteasome regulatory particle assembly molecular_function cytosol|nucleus|proteasome regulatory particle YPL009C TAE2 hom FT TRANSLATION 0.0176700394002488 0.306362861002742 943 Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm cytoplasmic translation molecular_function ribosome|cytoplasm YNR055C HOL1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0176059588451534 0.308048386659222 944 Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake organic alcohol transport|cation transport alcohol transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YLR241W_p YLR241W hom 0.0176050385605155 0.308145860156167 945 Putative protein of unknown function, may be involved in detoxification biological_process molecular_function integral to membrane YLR306W UBC12 hom 0.0176013574148483 0.308608283210897 946 Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes protein neddylation NEDD8 ligase activity cellular_component YJL130C URA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0176007612595735 0.308191245631728 947 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP glutamine metabolic process|'de novo' pyrimidine nucleobase biosynthetic process|negative regulation of pyrimidine nucleobase metabolic process aspartate carbamoyltransferase activity|carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity mitochondrion|integral to membrane|cytoplasm YGR018C_p YGR018C hom 0.0175690168565708 0.309064711615045 948 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component YMR260C TIF11 het FT TRANSLATION|RIBOSOME 0.0175665465837657 0.309132751151188 949 Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 translational initiation double-stranded RNA binding|translation initiation factor activity ribosome YLL056C_p YLL056C hom 0.0175222015420254 0.310500253466837 950 Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component YLR429W CRN1 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0175214967671213 0.31037530872486 951 Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly microtubule-based process|actin filament organization|actin cortical patch localization|Unknown|negative regulation of Arp2/3 complex-mediated actin nucleation microtubule binding|actin filament binding|protein binding, bridging Arp2/3 protein complex|actin cortical patch YPL194W DDC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0175050994154324 0.310900590641757 952 DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress meiosis|mitotic cell cycle G1/S transition checkpoint|intra-S DNA damage checkpoint|recombinational repair|G2/M transition checkpoint|cell cycle checkpoint molecular_function condensed nuclear chromosome|checkpoint clamp complex YOR284W HUA2 hom 0.0174940593972998 0.311133694882956 953 Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YPL243W SRP68 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0174912917544792 0.311643772060903 954 Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding signal recognition particle, endoplasmic reticulum targeting YPL095C EEB1 hom FT KETONE METABOLISM|LIPID METABOLISM 0.0174672255913325 0.311876578985156 955 Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication medium-chain fatty acid catabolic process|medium-chain fatty acid biosynthetic process short-chain carboxylesterase activity|alcohol O-octanoyltransferase activity cellular_component YPL271W ATP15 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0174476089533121 0.313650784951643 956 Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, central stalk YMR242C RPL20A hom FF|FT TRANSLATION|RIBOSOME 0.0174037691265715 0.313710198356303 957 Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YGL005C COG7 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.017403735526407 0.314289550641651 958 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function mitochondrion|Golgi transport complex YLR405W DUS4 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS 0.017399683581605 0.314040572612466 959 Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p tRNA modification tRNA dihydrouridine synthase activity cellular_component YHR065C RRP3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0173978338031284 0.313803079737364 960 Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA-dependent ATPase activity|ATP-dependent RNA helicase activity nucleolus YNL026W SAM50 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0173598973178414 0.314859599335109 961 Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 protein complex assembly|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion|integral to mitochondrial outer membrane YIL001W_p YIL001W hom 0.0173586087501935 0.314895526541242 962 Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm biological_process molecular_function cytoplasm YLR063W_p YLR063W hom 0.0173522160503761 0.315073804458069 963 Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cytoplasm YHL013C OTU2 hom 0.0173513109105791 0.315099052113422 964 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|cytoplasm YMR012W CLU1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION 0.0173490293412058 0.31516269931384 965 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation|mitochondrion organization molecular_function eukaryotic translation initiation factor 3 complex|cytoplasm YPL046C ELC1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0173484517143882 0.315323250220093 966 Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair global genome nucleotide-excision repair|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YIR008C PRI1 het FT NUCLEUS|CHROMOSOME 0.0173052087678828 0.316386766733563 967 Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA replication initiation|DNA replication single-stranded DNA binding|DNA primase activity alpha DNA polymerase:primase complex|nuclear replication fork YOR264W DSE3 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH 0.0172923694141138 0.316746006369908 968 Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud neck YDR513W GRX2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.017286175231795 0.316919412556832 969 Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication cellular response to oxidative stress disulfide oxidoreductase activity|glutathione transferase activity|glutathione peroxidase activity cytosol|mitochondrion YLR166C SEC10 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.017274276590644 0.317252689702022 970 Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion exocytosis|establishment of cell polarity|Golgi to plasma membrane transport|vesicle fusion|vesicle docking involved in exocytosis molecular_function mating projection tip|exocyst YBL066C SEF1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.017251984445093 0.317877703697729 971 Putative transcription factor, has homolog in Kluyveromyces lactis biological_process molecular_function cellular_component YOL146W PSF3 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0172503680695063 0.317923054020488 972 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function replication fork protection complex|GINS complex|DNA replication preinitiation complex YDR324C UTP4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0172180244311073 0.318831403689431 973 Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|t-UTP complex|90S preribosome YBR002C RER2 het FF|FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0172131744298725 0.319329012067988 974 Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting dolichol biosynthetic process|protein glycosylation|ER to Golgi vesicle-mediated transport dehydrodolichyl diphosphate synthase activity|prenyltransferase activity endoplasmic reticulum YGR210C_p YGR210C hom 0.0172066719724626 0.31915063225532 975 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YBR266C_d SLM6 hom 0.0172033822541546 0.319243177451482 976 Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Unknown Unknown Unknown YML114C TAF8 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0171934778312094 0.319521911162493 977 TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YLR039C RIC1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.0171925451486287 0.319548167236364 978 Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes intracellular protein transport|retrograde transport, endosome to Golgi guanyl-nucleotide exchange factor activity nucleus|Ric1p-Rgp1p complex|Golgi membrane|guanyl-nucleotide exchange factor complex YGR264C MES1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0171830195264912 0.31981640510518 979 Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs methionyl-tRNA aminoacylation methionine-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|cytoplasm YPL071C_p YPL071C hom FT NUCLEUS 0.0171745102532037 0.320056147429792 980 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YNR069C BSC5 hom 0.0171132765887119 0.321857024927991 981 Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro biological_process molecular_function cellular_component YIR024C YIR024C hom FT MITOCHONDRION 0.0170933924192729 0.322347476575028 982 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect biological_process molecular_function mitochondrion YDR362C TFC6 het FT NUCLEUS 0.0170916705618802 0.322757445153099 983 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity transcription factor TFIIIC complex YER083C GET2 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0170757339891594 0.322847687257085 984 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division retrograde vesicle-mediated transport, Golgi to ER|protein insertion into ER membrane protein anchor integral to membrane|endoplasmic reticulum|GET complex YFL011W HXT10 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.0170473923059928 0.323651579767315 985 Putative hexose transporter, expressed at low levels and expression is repressed by glucose hexose transport|transmembrane transport galactose transmembrane transporter activity|mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YMR243C ZRC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0170242027509087 0.32431030459698 986 Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication cobalt ion transport|cellular zinc ion homeostasis|zinc ion transport|glutathione metabolic process zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity integral to membrane|fungal-type vacuole YOR073W SGO1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0170198852717128 0.324433043635308 987 Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability positive regulation of maintenance of meiotic sister chromatid cohesion|meiotic sister chromatid segregation|meiotic sister chromatid separation|sister chromatid biorientation|maintenance of meiotic sister chromatid cohesion|mitotic cell cycle spindle assembly checkpoint|mitotic sister chromatid segregation molecular_function condensed nuclear chromosome kinetochore|nucleus|spindle pole body|condensed nuclear chromosome, centromeric region YNL126W SPC98 het FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0169712992269869 0.32581635124399 988 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation|mitotic spindle organization in nucleus structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YBR202W MCM7 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0169590983119961 0.326164328372015 989 Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex S phase of mitotic cell cycle|regulation of transcription from RNA polymerase II promoter|DNA replication initiation|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|pre-replicative complex assembly|double-strand break repair via break-induced replication|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere DNA replication origin binding|single-stranded DNA-dependent ATPase activity|transcription cofactor activity|single-stranded DNA binding|ATP-dependent 3'-5' DNA helicase activity|ATP binding|four-way junction helicase activity|DNA helicase activity|chromatin binding replication fork protection complex|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YPR194C OPT2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0169545155500264 0.326367277468801 990 Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles oligopeptide transport|vacuole fusion, non-autophagic oligopeptide transporter activity integral to membrane|integral to plasma membrane YOL077C BRX1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0169369791539771 0.326795794918583 991 Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif ribosomal large subunit assembly 5S rRNA binding|rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YOR287C RRP36 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0169362608477028 0.326816314710886 992 Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cleavage involved in rRNA processing molecular_function nucleolus|90S preribosome YLR300W EXG1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0169210892087959 0.327249917012671 993 Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes fungal-type cell wall organization|cellular glucan metabolic process glucan exo-1,3-beta-glucosidase activity extracellular region|fungal-type cell wall YLR440C SEC39 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0169125468441528 0.327494220236882 994 Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope retrograde vesicle-mediated transport, Golgi to ER|vesicle-mediated transport|ER-dependent peroxisome organization molecular_function Dsl1p complex|nuclear envelope|endoplasmic reticulum|peroxisome|endoplasmic reticulum membrane YOR031W CRS5 hom 0.0169124904483293 0.327495833495037 995 Copper-binding metallothionein, required for wild-type copper resistance response to metal ion copper ion binding cytoplasm YHR042W NCP1 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.0169083677815693 0.327613780409489 996 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p ergosterol biosynthetic process electron carrier activity mitochondrial outer membrane|mitochondrion YMR055C BUB2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON 0.0169025684200308 0.327779743153912 997 Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage regulation of exit from mitosis|mitotic cell cycle spindle orientation checkpoint GTPase activator activity spindle pole body YML010W SPT5 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0168789404570703 0.328456478000921 998 Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair 7-methylguanosine mRNA capping|intracellular mRNA localization|mRNA splicing, via spliceosome|positive regulation of transcription elongation from RNA polymerase II promoter|Unknown|regulation of transcription-coupled nucleotide-excision repair core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rRNA binding|RNA polymerase II core binding|RNA polymerase I transcription factor binding|RNA polymerase I core binding DSIF complex|mitochondrion|rDNA heterochromatin|nucleus YOR298C-A MBF1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0168707803415453 0.328762573264561 999 Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity mitochondrion|nucleus YCR065W HCM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0168688870409445 0.3287446950557 1000 Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role positive regulation of transcription involved in S-phase of mitotic cell cycle|cellular response to oxidative stress|mitochondrion organization|spindle pole body organization|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nuclear chromatin|nucleus|cytoplasm YOR382W FIT2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0168566839202273 0.329094761196415 1001 Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall siderophore transport molecular_function fungal-type cell wall YDR520C_p URC2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM|NUCLEUS 0.0168456659360848 0.329699818628167 1002 Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism uracil catabolic process sequence-specific DNA binding nucleus|cytoplasm YFL028C CAF16 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM|NUCLEUS 0.0168386770204758 0.329611758957831 1003 Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator regulation of transcription from RNA polymerase II promoter ATPase activity CCR4-NOT complex|cytoplasm YHR122W CIA2 het FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS 0.0168216409248945 0.330101367695716 1004 Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B establishment of mitotic sister chromatid cohesion molecular_function nucleus|cytoplasm YMR272W-B_p YMR272W-B hom 0.0167940260654103 0.33089600396041 1005 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YGL007C-A_p YGL007C-A hom 0.0167674020349897 0.331663298594749 1006 Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding biological_process molecular_function cellular_component YHR022C_p YHR022C hom FT SIGNALING 0.0167557491977954 0.331999490422658 1007 Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component YLR010C TEN1 het FT NUCLEUS|CHROMOSOME 0.0167524319384385 0.332095235718233 1008 Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p negative regulation of telomere maintenance|telomere capping|telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YJL048C UBX6 hom FT PROTEOLYSIS|NUCLEUS 0.0167171399753152 0.333114963484772 1009 UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication sporulation resulting in formation of a cellular spore|ubiquitin-dependent protein catabolic process molecular_function nucleus YKR055W RHO4 hom FT SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0166984971527352 0.333654444707853 1010 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity positive regulation of formin-nucleated actin cable assembly|maintenance of cell polarity|regulation of formin-nucleated actin cable assembly GTPase activity incipient cellular bud site|plasma membrane|cellular bud neck YDR070C FMP16 hom FT MITOCHONDRION 0.0166960962617661 0.333723962002429 1011 Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion YLL055W YCT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0166738618454085 0.334368198548628 1012 High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene cysteine transport ion transmembrane transporter activity|cysteine transmembrane transporter activity integral to membrane|endoplasmic reticulum YOR002W ALG6 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0166579257979753 0.334830434149872 1013 Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation|aerobic respiration transferase activity, transferring hexosyl groups|dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum YIL160C POT1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0166276149194445 0.335710756523745 1014 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids fatty acid beta-oxidation mRNA binding|acetyl-CoA C-acyltransferase activity peroxisomal matrix|peroxisome YOR008C-A_p YOR008C-A hom 0.0166212647547308 0.336111748619616 1015 Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres biological_process molecular_function cellular_component YOR143C THI80 het FT COFACTOR METABOLISM 0.0166073249680897 0.336300871361966 1016 Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) thiamine diphosphate biosynthetic process thiamine diphosphokinase activity cytoplasm YLR222C UTP13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0165901583840651 0.336800666546577 1017 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YHR059W FYV4 hom FT MITOCHONDRION 0.0165653001372355 0.337525245861157 1018 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function mitochondrion YML111W BUL2 hom FT UBIQUITIN LIGASE COMPLEX 0.0165416818733923 0.338214608486906 1019 Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication protein polyubiquitination|protein monoubiquitination ubiquitin-ubiquitin ligase activity ubiquitin ligase complex|cytoplasm YLR224W_p YLR224W hom FT PROTEOLYSIS 0.0165331794596782 0.338462995397919 1020 F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YLL050C COF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0165270798868324 0.338713330305883 1021 Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes actin filament depolymerization|actin filament severing actin filament binding plasma membrane|actin cortical patch YOR348C PUT4 hom FT PLASMA MEMBRANE 0.0165216210731522 0.33880084624467 1022 Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells neutral amino acid transport|proline transport|gamma-aminobutyric acid transport neutral amino acid transmembrane transporter activity|L-proline transmembrane transporter activity integral to membrane|plasma membrane YGL032C AGA2 hom 0.0165190930158413 0.33909103819535 1023 Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds agglutination involved in conjugation with cellular fusion cell adhesion molecule binding fungal-type cell wall YKL191W DPH2 hom 0.0165018990714131 0.339377818314507 1024 Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YIL004C BET1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0164836737877054 0.339911563236704 1025 Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum membrane|ER to Golgi transport vesicle YKL084W HOT13 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0164601076405765 0.340602517429091 1026 Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein import into mitochondrial intermembrane space zinc ion binding mitochondrial intermembrane space YKL058W TOA2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.016449708027352 0.340907717585768 1027 TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|TBP-class protein binding RNA polymerase II transcription factor activity transcription factor TFIIA complex YHL019C APM2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0164490727748075 0.340926366179151 1028 Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YML068W ITT1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION 0.0164376691736594 0.341261243513603 1029 Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins regulation of translational termination molecular_function cellular_component YDR105C TMS1 hom 0.0163948273946338 0.342521212120053 1030 Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance biological_process molecular_function fungal-type vacuole membrane|integral to membrane|membrane YOR101W RAS1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0163929618590218 0.342648162385621 1031 GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress adenylate cyclase-activating G-protein coupled receptor signaling pathway|replicative cell aging|Ras protein signal transduction GTPase activity plasma membrane YML030W RCF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0163457726530824 0.344039545359757 1032 Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast mitochondrial respiratory chain complex IV assembly|mitochondrial respiratory chain supercomplex assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|integral to mitochondrial inner membrane|mitochondrial respiratory chain complex IV YJL031C BET4 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION 0.0163408582091574 0.344833521830488 1033 Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YOR127W RGA1 hom FT SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0163247142117046 0.34458963259299 1034 GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress budding cell isotropic bud growth|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction|invasive growth in response to glucose limitation|budding cell apical bud growth Rho GTPase activator activity cellular bud neck split septin rings YKR045C_p YKR045C hom 0.0163217983246238 0.345396596985708 1035 Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm YPR061C JID1 hom FT MITOCHONDRION 0.0162988207086773 0.345355530996807 1036 Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae biological_process molecular_function mitochondrion YER056C FCY2 hom FT PLASMA MEMBRANE 0.0162804126378229 0.345900678585698 1037 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress cytosine transport|cytidine transport|purine nucleobase transport|transmembrane transport nucleobase transmembrane transporter activity|cytidine transmembrane transporter activity integral to membrane|plasma membrane YIL141W_d YIL141W hom 0.0162658154216291 0.346333358634505 1038 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR071C NRT1 hom FT PLASMA MEMBRANE 0.0162246487904418 0.347555444936412 1039 High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity thiamine transport|nicotinamide riboside transport nicotinamide riboside transporter activity integral to membrane|plasma membrane YKL006W RPL14A hom FT TRANSLATION|RIBOSOME 0.016212906917756 0.347976450904728 1040 Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YJR024C MDE1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0162100553587693 0.347989327840994 1041 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant L-methionine salvage from methylthioadenosine methylthioribulose 1-phosphate dehydratase activity cytoplasm YDR403W DIT1 hom FT CELL WALL ORG/BIOGENESIS 0.0162100070778833 0.348206612553964 1042 Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure ascospore wall assembly catalytic activity cellular_component YIL034C CAP2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0162021953065797 0.348223160628461 1043 Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping|filamentous growth actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YGL095C VPS45 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0162013217321018 0.348464990003503 1044 Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment vacuole inheritance|CVT pathway|positive regulation of SNARE complex assembly|Golgi vesicle fusion to target membrane|Golgi to vacuole transport|vacuole organization|vacuolar acidification|protein targeting to vacuole|Golgi to endosome transport unfolded protein binding|SNARE binding cytosol|SNARE complex|Golgi membrane|extrinsic to membrane YKL135C APL2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0161983663102662 0.348337107587548 1045 Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YDR344C_d YDR344C hom 0.0161957235636476 0.348415766800707 1046 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR137C PSO2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0161797334008929 0.348891941690236 1047 Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress DNA repair|double-strand break repair damaged DNA binding|5'-3' exonuclease activity nucleus YLR189C ATG26 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM 0.0161781531305776 0.348939023363696 1048 UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy sterol metabolic process|ascospore-type prospore membrane assembly sterol 3-beta-glucosyltransferase activity cytoplasm YEL039C CYC7 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0161633166694307 0.349597021761845 1049 Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen|mitochondrial electron transport, ubiquinol to cytochrome c electron carrier activity mitochondrion|mitochondrial intermembrane space YCR090C_p YCR090C hom FT NUCLEUS 0.0161551732477416 0.349983827702017 1050 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus|cytoplasm YOR370C MRS6 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|PROTEIN PRENYLATION 0.0161405400201653 0.350060837388656 1051 Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport|activation of Rab GTPase activity Rab geranylgeranyltransferase activity|Rab GTPase binding Rab-protein geranylgeranyltransferase complex|membrane|cytoplasm YGL080W FMP37 hom FT MITOCHONDRION 0.0161331756950288 0.350280746417806 1052 Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|integral to mitochondrial inner membrane YGL108C YGL108C hom 0.0161057815185038 0.351387149304684 1053 Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress biological_process molecular_function cellular_component YNL212W VID27 hom 0.0160855352042528 0.351705473289864 1054 Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth biological_process molecular_function cytoplasm YDR488C PAC11 hom FT CYTOSKELETON 0.0160701811446684 0.352165429309059 1055 Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 nuclear migration along microtubule|microtubule-based process plus-end-directed microtubule motor activity|microtubule motor activity cytoplasmic dynein complex|cytoplasmic microtubule YGR112W SHY1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0160641932534173 0.35234490952453 1056 Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane YPR192W AQY1 hom FT PLASMA MEMBRANE 0.0160601705741838 0.35253736149192 1057 Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance ascospore formation|water transport water channel activity integral to membrane|plasma membrane YNL220W ADE12 hom FT NUCLEOTIDE METABOLISM 0.0160552046945788 0.352614439946865 1058 Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence purine nucleotide biosynthetic process DNA replication origin binding|adenylosuccinate synthase activity cytoplasm YFL024C EPL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0160215721588934 0.354919925826862 1059 Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YDR510W SMT3 het FT NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0160186739454334 0.353711190430051 1060 Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 protein sumoylation protein tag condensed nuclear chromosome|nucleus|septin ring YDR076W RAD55 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0160093366664059 0.353991865896445 1061 Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p DNA recombinase assembly|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YKL163W PIR3 hom FT CELL WALL ORG/BIOGENESIS 0.0159932136853379 0.354476848332021 1062 O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall YOL011W PLB3 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0159724311365645 0.355174398993696 1063 Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro phosphatidylinositol metabolic process|phosphatidylserine catabolic process lysophospholipase activity extracellular region|plasma membrane YER158C_p YER158C hom 0.0159657966228033 0.355302521508913 1064 Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication biological_process molecular_function cellular_component YBL100W-C_p YBL100W-C hom 0.0159495876398923 0.356150298712828 1065 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR112W_d IRC2 hom 0.0159423974236632 0.357591672661126 1066 Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Unknown Unknown Unknown YGR091W PRP31 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0159405744774585 0.356063164413202 1067 Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome|spliceosomal snRNP assembly molecular_function mitochondrion|U4/U6 x U5 tri-snRNP complex YGR243W FMP43 hom FT MITOCHONDRION 0.0159348168697744 0.356236944618911 1068 Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|mitochondrial membrane YLR075W RPL10 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0159239906272537 0.35656385465731 1069 Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects cytoplasmic translation|translational termination|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YMR037C MSN2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0159072642983614 0.3570692955208 1070 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|cellular response to methylmercury|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YGR001C_p YGR001C hom 0.015900057342534 0.357646116811703 1071 Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae biological_process methyltransferase activity cytoplasm YIL104C SHQ1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0158704675975012 0.358182815209765 1072 Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones box H/ACA snoRNP assembly unfolded protein binding nucleoplasm YJL002C OST1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0158513215509313 0.358763064118449 1073 Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation via asparagine|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YKL107W_p YKL107W hom FT OXIDATION-REDUCTION PROCESS 0.0158373248731341 0.359187627449514 1074 Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein biological_process molecular_function cellular_component YCL055W KAR4 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0158332966742816 0.359525013047128 1075 Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone karyogamy involved in conjugation with cellular fusion|meiosis|negative regulation of transcription from RNA polymerase II promoter by pheromones|positive regulation of transcription from RNA polymerase II promoter by pheromones|G1 phase of mitotic cell cycle sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YEL022W GEA2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0158173571000342 0.359793858958509 1076 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|secretory granule organization|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|extrinsic to membrane YDR050C TPI1 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0158063216085828 0.360200851041494 1077 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease glycolysis triose-phosphate isomerase activity mitochondrion|plasma membrane|cytoplasm YMR180C CTL1 hom FT RNA PROCESSING|NUCLEUS 0.0157974553537137 0.360398724607901 1078 RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm biological_process polynucleotide 5'-phosphatase activity nucleus|cytoplasm YDL171C GLT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0157970648458848 0.360410599531063 1079 NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source glutamate biosynthetic process|ammonia assimilation cycle glutamate synthase (NADH) activity mitochondrion YMR061W RNA14 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.015792077292578 0.360562287251898 1080 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation RNA binding|protein heterodimerization activity mRNA cleavage factor complex|mitochondrion|nucleus YOR021C SFM1 hom 0.0157904110038368 0.360612973436031 1081 SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism peptidyl-arginine methylation protein-arginine omega-N monomethyltransferase activity cellular_component YJR135W-A TIM8 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0157722429155001 0.361165911380857 1082 Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome protein import into mitochondrial inner membrane protein transporter activity mitochondrion|integral to membrane|mitochondrial intermembrane space protein transporter complex YLR083C EMP70 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.0157415511846433 0.362101207544354 1083 Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments cellular copper ion homeostasis|vacuolar transport|pseudohyphal growth|endosomal transport|invasive growth in response to glucose limitation molecular_function endosome|fungal-type vacuole membrane|integral to membrane YHL006C SHU1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0157391406035294 0.362174731498036 1084 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function recombinational repair|maintenance of rDNA molecular_function nucleolus|Shu complex YGL020C GET1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0157387281925625 0.362187311179351 1085 Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance retrograde vesicle-mediated transport, Golgi to ER|protein insertion into ER membrane protein anchor mitochondrion|integral to membrane|endoplasmic reticulum|GET complex YGL214W_d YGL214W hom 0.0156919400217284 0.363616256511261 1086 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Unknown Unknown Unknown YNL077W APJ1 hom FT NUCLEUS|MITOCHONDRION 0.015686060953134 0.363796056571775 1087 Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress biological_process unfolded protein binding mitochondrion|nucleus|cytoplasm YNL168C_p FMP41 hom FT MITOCHONDRION 0.0156558117849903 0.364722047907437 1088 Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOR336W KRE5 het FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOPLASMIC RETICULUM 0.0156371269384412 0.365294765552691 1089 Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects (1->6)-beta-D-glucan biosynthetic process UDP-glucose:glycoprotein glucosyltransferase activity endoplasmic reticulum YLR038C COX12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0156270778502886 0.365603016673286 1090 Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV mitochondrial respiratory chain complex IV assembly cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDL090C RAM1 hom FF|FT PROTEIN PRENYLATION 0.0156226128594749 0.367246232932316 1091 Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit protein farnesylation protein farnesyltransferase activity protein farnesyltransferase complex YGL068W MNP1 het FT TRANSLATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0156204857404999 0.365805314711477 1092 Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth aerobic respiration structural constituent of ribosome mitochondrial nucleoid|mitochondrion YIL044C AGE2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.0155917996178266 0.366757947939323 1093 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport ARF GTPase activator activity clathrin-coated vesicle YER087C-B SBH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0155840618495606 0.366924346024627 1094 Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation ARF guanyl-nucleotide exchange factor activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex YML080W DUS1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0155837147834491 0.366935018995512 1095 Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 tRNA modification tRNA dihydrouridine synthase activity nucleus YDR472W TRS31 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0155771876775772 0.367135776463667 1096 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YOR050C_d YOR050C hom 0.015563311328061 0.367562805604196 1097 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable Unknown Unknown Unknown YGR176W_d YGR176W hom 0.0155371404943806 0.368511956637025 1098 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR253C_p YMR253C hom 0.0155045795150687 0.369373633956973 1099 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function integral to membrane|cytoplasm YMR207C HFA1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0155022164738457 0.36944660744066 1100 Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress fatty acid biosynthetic process acetyl-CoA carboxylase activity mitochondrion YOR033C EXO1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0154926897952212 0.369740893298627 1101 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress mismatch repair|telomeric 3' overhang formation|DNA double-strand break processing|meiotic DNA double-strand break processing|telomere maintenance|gene conversion at mating-type locus, DNA double-strand break processing 5'-3' exonuclease activity|5'-flap endonuclease activity nucleus YMR006C PLB2 hom FT LIPID METABOLISM 0.0154861685461776 0.369942423239436 1102 Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine glycerophospholipid metabolic process lysophospholipase activity extracellular region|fungal-type cell wall YFL056C AAD6 hom FT OXIDATION-REDUCTION PROCESS 0.0154774115483969 0.370213153112578 1103 Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YDR247W VHS1 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION 0.0154502734705411 0.371767573161356 1104 Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p protein phosphorylation|G1/S transition of mitotic cell cycle protein serine/threonine kinase activity|protein kinase activity cytoplasm YLR436C ECM30 hom FT CELL WALL ORG/BIOGENESIS 0.0154485081759484 0.371107597677654 1105 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization molecular_function cytoplasm YKL017C HCS1 hom FT NUCLEUS|CHROMOSOME 0.0154341418870477 0.371552675551526 1106 Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities lagging strand elongation ATP-dependent 5'-3' DNA helicase activity DNA helicase A complex|alpha DNA polymerase:primase complex YNL183C NPR1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|GOLGI APPARATUS|PLASMA MEMBRANE 0.015414650302285 0.372157067617745 1107 Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex regulation of transmembrane transporter activity|regulation of nitrogen utilization|protein phosphorylation|negative regulation of endocytosis protein serine/threonine kinase activity Golgi apparatus|plasma membrane|cytoplasm YHR023W MYO1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0153779657145648 0.373296228668941 1108 Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively cytokinesis, actomyosin contractile ring assembly|cytokinesis, completion of separation|actomyosin contractile ring contraction|cytokinesis microfilament motor activity cellular bud neck contractile ring|incipient cellular bud site|cellular bud neck|myosin II complex YCR024C-A PMP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0153759484668434 0.373358932492466 1109 Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication cation transport enzyme regulator activity plasma membrane|membrane YKL125W RRN3 het FT NUCLEUS 0.0153687375581655 0.373583128574613 1110 Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo transcription initiation from RNA polymerase I promoter for nuclear large rRNA transcript core RNA polymerase I binding transcription factor activity|RNA polymerase I transcription factor binding transcription factor activity|RNA polymerase I transcription factor binding|RNA polymerase I core binding nucleolus YOL101C IZH4 hom FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0153614388724133 0.373810138550458 1111 Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress cellular zinc ion homeostasis|cellular lipid metabolic process metal ion binding integral to membrane|membrane YLR413W_p YLR413W hom FT PLASMA MEMBRANE 0.0153546324819597 0.374378539661252 1112 Putative protein of unknown function; YLR413W is not an essential gene biological_process molecular_function integral to membrane YNL255C GIS2 hom FT TRANSLATION|RIBOSOME 0.0153538713094247 0.374045601448028 1113 Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 positive regulation of translation mRNA binding|translation regulator activity|single-stranded DNA binding polysomal ribosome|cytoplasm YKL142W MRP8 hom 0.0153472051549405 0.374253093196758 1114 Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis biological_process molecular_function cytoplasm YDR307W_p PMT7 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0153316054477615 0.374738930104674 1115 Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation biological_process molecular_function integral to membrane|endoplasmic reticulum YHR049W FSH1 hom FT NUCLEUS 0.0153245410032064 0.374959073137973 1116 Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process hydrolase activity nucleus|cytoplasm YHR088W RPF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0153080438514503 0.37561597155967 1117 Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit assembly|ribosomal large subunit export from nucleus|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YHR112C YHR112C hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0152843116137416 0.376214228263378 1118 Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway sulfur compound metabolic process cystathionine beta-lyase activity nucleus|cytoplasm YGR259C_d YGR259C hom 0.0152772141915462 0.376649623272724 1119 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Unknown Unknown Unknown YHR150W PEX28 hom FT PEROXISOME ORGANIZATION 0.0152601274544367 0.377041211553977 1120 Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p peroxisome organization molecular_function integral to membrane|peroxisomal membrane YAL037W_p YAL037W hom 0.0152578128163409 0.377042403793184 1121 Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication biological_process molecular_function cellular_component YJR130C STR2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0152362260294183 0.378216465535539 1122 Cystathionine gamma-synthase, converts cysteine into cystathionine sulfur compound metabolic process|transsulfuration cystathionine gamma-synthase activity nucleus|cytoplasm YGL115W SNF4 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE 0.0152132061011716 0.378581354330847 1123 Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress positive regulation of gluconeogenesis|peroxisome organization|protein phosphorylation|replicative cell aging|regulation of transcription from RNA polymerase II promoter AMP-activated protein kinase activity|protein serine/threonine kinase activator activity AMP-activated protein kinase complex|plasma membrane|nucleus|nuclear envelope lumen|cytoplasm YNL035C YNL035C hom FT NUCLEUS 0.0151999200622776 0.378855650167457 1124 Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YMR120C ADE17 hom FT NUCLEOTIDE METABOLISM 0.0151958479080552 0.378983394836723 1125 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol|plasma membrane YJL138C TIF2 hom FT TRANSLATION|RIBOSOME 0.0151597862753733 0.380115814622598 1126 Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication translational initiation|regulation of translational initiation ATP-dependent RNA helicase activity|translation initiation factor activity ribosome|eukaryotic translation initiation factor 4F complex|plasma membrane|cytoplasm YDL150W RPC53 het FF|FT NUCLEUS 0.0151451464376911 0.380576132451438 1127 RNA polymerase III subunit C53 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YNL118C DCP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0151389604489419 0.380770740227901 1128 Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|deadenylation-independent decapping of nuclear-transcribed mRNA|stress granule assembly hydrolase activity|mRNA binding|m7G(5')pppN diphosphatase activity nucleus|cytoplasmic mRNA processing body|cytoplasm YOR326W MYO2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0151339939875472 0.380998085777047 1129 Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication vacuole inheritance|establishment of mitotic spindle orientation|peroxisome inheritance|budding cell apical bud growth|unidimensional cell growth|mitochondrion inheritance|membrane addition at site of cytokinesis|vesicle transport along actin filament|vesicle-mediated transport|Golgi inheritance microfilament motor activity|actin filament binding|calmodulin binding fungal-type vacuole membrane|incipient cellular bud site|filamentous actin|myosin V complex|actin filament bundle|cellular bud neck|cellular bud tip|vesicle|mating projection tip YGL226C-A OST5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0150967019323381 0.382101803188847 1130 Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YIL074C SER33 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0150964622584059 0.382109360587354 1131 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication serine family amino acid biosynthetic process phosphoglycerate dehydrogenase activity cytoplasm YLL010C PSR1 hom FT PLASMA MEMBRANE 0.0150034407383892 0.385049430540928 1132 Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p response to stress phosphoprotein phosphatase activity plasma membrane YDR363W-A SEM1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.0150009943334081 0.385126938530214 1133 Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|regulation of cell cycle|mRNA export from nucleus|exocytosis|filamentous growth|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|proteasome storage granule|proteasome regulatory particle, lid subcomplex YLR008C PAM18 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0150001208204473 0.385154615829427 1134 Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p protein import into mitochondrial matrix unfolded protein binding|ATPase activator activity mitochondrion|presequence translocase-associated import motor|mitochondrial inner membrane presequence translocase complex YHL004W MRP4 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0149928312071368 0.385385634952121 1135 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome|tRNA binding mitochondrion|mitochondrial small ribosomal subunit YGL207W SPT16 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0149769436474215 0.385889429464949 1136 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome DNA replication-independent nucleosome organization|regulation of transcription by chromatin organization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription initiation from RNA polymerase II promoter|nucleosome assembly|transcription elongation from RNA polymerase II promoter|DNA-dependent DNA replication chromatin binding|nucleosome binding|histone binding nuclear chromatin|FACT complex|replication fork protection complex|alpha DNA polymerase:primase complex|transcription elongation factor complex YMR235C RNA1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|GENE SILENCING|NUCLEUS 0.0149721107470743 0.38604276044795 1137 GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport rRNA metabolic process|ribosomal subunit export from nucleus|tRNA metabolic process|rRNA export from nucleus|RNA import into nucleus|protein import into nucleus|chromatin silencing at telomere Ran GTPase activator activity cytosol|integral to membrane|nucleus YGL255W ZRT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0149468574225963 0.387198977842214 1138 High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor high-affinity zinc ion transport high affinity zinc uptake transmembrane transporter activity integral to membrane|integral to plasma membrane YOR280C FSH3 hom 0.014941008802578 0.387030405523556 1139 Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cellular_component YDL049C KNH1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0149400192479521 0.387132691694187 1140 Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance (1->6)-beta-D-glucan biosynthetic process molecular_function fungal-type cell wall|extracellular region YLR082C SRL2 hom FT NUCLEUS 0.0149314957252495 0.387332801559021 1141 Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation nucleobase-containing compound metabolic process molecular_function nucleus|cytoplasm YDL195W SEC31 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0149150763501001 0.387855069530038 1142 Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance COPII-coated vesicle budding structural molecule activity COPII vesicle coat|mating projection tip YDL055C PSA1 het FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0149094210920575 0.388035051416804 1143 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure protein glycosylation|cell wall mannoprotein biosynthetic process|GDP-mannose biosynthetic process mannose-1-phosphate guanylyltransferase activity cytoplasm YJL071W ARG2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.014896304291627 0.388452697006735 1144 Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p arginine biosynthetic process|ornithine biosynthetic process acetyl-CoA:L-glutamate N-acetyltransferase activity mitochondrial matrix YBL018C POP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0148758797640603 0.389457551743589 1145 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YPR057W BRR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0148735765559659 0.389177007197934 1146 snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed spliceosomal complex assembly RNA binding small nuclear ribonucleoprotein complex YJL090C DPB11 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.0148530636468012 0.389831437884782 1147 DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress lagging strand elongation|recombinational repair|nucleotide-excision repair|DNA replication initiation|mismatch repair|positive regulation of protein phosphorylation|mating type switching|DNA replication checkpoint|double-strand break repair via break-induced replication|mitotic cell cycle G2/M transition DNA damage checkpoint|leading strand elongation protein kinase activator activity|protein binding DNA replication preinitiation complex|replication fork|epsilon DNA polymerase complex YBR160W CDC28 het FT CELL CYCLE|LIPID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0148441387121605 0.390116381972055 1148 Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress regulation of budding cell apical bud growth|negative regulation of meiotic cell cycle|positive regulation of triglyceride catabolic process|protein phosphorylation|regulation of filamentous growth|negative regulation of transcription, DNA-dependent|positive regulation of meiotic cell cycle|positive regulation of transcription, DNA-dependent|regulation of double-strand break repair via homologous recombination|vesicle-mediated transport|positive regulation of DNA replication involved in S phase|positive regulation of mitotic cell cycle|positive regulation of spindle pole body separation|chromatin remodeling|negative regulation of mitotic cell cycle histone binding|protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|ribosome|endoplasmic reticulum|nucleus|spindle pole body|cellular bud neck|astral microtubule|cytoplasm YJR053W BFA1 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON 0.0148369226688708 0.390346859227476 1149 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis regulation of exit from mitosis|mitotic cell cycle spindle orientation checkpoint GTPase activator activity spindle pole body YGR163W GTR2 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|MEMBRANE ORGANIZATION|NUCLEUS|CHROMOSOME 0.0148126919157428 0.391121383854451 1150 Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD microautophagy|positive regulation of transcription from RNA polymerase II promoter GTP binding nuclear chromatin|fungal-type vacuole membrane|integral to membrane|EGO complex|nucleus|late endosome membrane|cytoplasm YJL102W MEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0147650192494382 0.392647938602069 1151 Mitochondrial elongation factor involved in translational elongation translational elongation translation elongation factor activity mitochondrion YOL118C_d YOL118C hom 0.014749210443315 0.393366811798907 1152 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER137C_p YER137C hom 0.0147418722831055 0.393390441554981 1153 Putative protein of unknown function biological_process molecular_function cellular_component YNL113W RPC19 het FT NUCLEUS 0.0147388156724107 0.393488554294648 1154 RNA polymerase subunit AC19, common to RNA polymerases I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex YGR140W CBF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0147296274858598 0.393783570842616 1155 Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress chromosome segregation|septin ring assembly|mitotic spindle elongation DNA binding, bending|centromeric DNA binding spindle midzone|condensed nuclear chromosome kinetochore|CBF3 complex|condensed nuclear chromosome, centromeric region|spindle YPR008W HAA1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0147229651486821 0.393997570492947 1156 Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress response to acid|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YOL034W SMC5 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0147175513902003 0.394242075041063 1157 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair DNA repair|resolution of recombination intermediates|recombinational repair|rDNA separation damaged DNA binding|single-stranded DNA binding|ATPase activity Smc5-Smc6 complex|nucleus YPL236C ENV7 hom FT PROTEIN PHOSPHORYLATION|VACUOLAR PROTEIN PROCESSING 0.014715403235252 0.394311106116881 1158 Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) vacuolar protein processing protein serine/threonine kinase activity fungal-type vacuole membrane YKL198C PTK1 hom FT PROTEIN PHOSPHORYLATION 0.0146926671689026 0.394971653323743 1159 Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein polyamine transport protein kinase activity cellular_component YKR027W BCH2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.0146795444147678 0.395394002393418 1160 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function trans-Golgi network transport vesicle|mitochondrion|clathrin-coated vesicle|exomer complex YGL172W NUP49 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0146749131796988 0.395543121410526 1161 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) poly(A)+ mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|nuclear envelope|integral to membrane|nuclear pore YGR169C PUS6 hom FT RNA PROCESSING|MITOCHONDRION 0.014652540685942 0.396263961345654 1162 tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|cytoplasm YGR282C BGL2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0146490900825823 0.396375210030641 1163 Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance fungal-type cell wall organization 1,3-beta-glucanosyltransferase activity|glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall YKR099W BAS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0146407262836166 0.404070570339117 1164 Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes positive regulation of transcription from RNA polymerase II promoter core promoter sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity nucleus YNL214W PEX17 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0146363979866348 0.39678456985894 1165 Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis protein import into peroxisome matrix, docking protein binding peroxisomal membrane YLL047W_d YLL047W hom 0.0146259056971524 0.39712317149342 1166 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Unknown Unknown Unknown YJL176C SWI3 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RSC COMPLEX 0.014614474701744 0.397492264770869 1167 Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions cellular alcohol catabolic process|ATP-dependent chromatin remodeling|positive regulation of mating type switching|sucrose catabolic process|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter histone binding|DNA binding SWI/SNF complex|nucleus YDR356W SPC110 het FT CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0146093687660883 0.397727609283099 1168 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner karyogamy involved in conjugation with cellular fusion|microtubule nucleation structural constituent of cytoskeleton central plaque of spindle pole body|inner plaque of spindle pole body YLR424W SPP382 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.014609209081749 0.397662354920204 1169 Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation spliceosomal complex disassembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step RNA binding|ATP-dependent RNA helicase activity mitochondrion|U2-type post-mRNA release spliceosomal complex|nucleus|spliceosomal complex|cytoplasm YLR261C_d VPS63 hom FF 0.0146066445909313 0.397745209025554 1170 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Unknown Unknown Unknown YMR058W FET3 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0146051651689724 0.397793011229791 1171 Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress iron assimilation by reduction and transport|high-affinity iron ion transport|response to copper ion ferroxidase activity|iron ion transmembrane transporter activity integral to membrane|high affinity iron permease complex|plasma membrane YKL062W MSN4 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0146016224810534 0.397907494526007 1172 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|cellular response to acidity|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|replicative cell aging|age-dependent response to oxidative stress involved in chronological cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YPL191C_p YPL191C hom 0.0146004721510165 0.397944672143112 1173 Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YOR334W MRS2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0145863884267314 0.398400014866763 1174 Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YOR265W RBL2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0145619991786419 0.399189288388726 1175 Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress tubulin complex assembly|tubulin complex biogenesis tubulin binding|beta-tubulin binding cytoplasm YIL079C AIR1 hom FT RNA PROCESSING|NUCLEUS 0.0145456652343909 0.399718405406771 1176 Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent snRNA catabolic process polynucleotide adenylyltransferase activity nucleolus|TRAMP complex YLL005C SPO75 hom FT CELL WALL ORG/BIOGENESIS 0.0145349860537849 0.400064571489203 1177 Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function integral to membrane YOR317W FAA1 hom FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0144787313400408 0.401891042392481 1178 Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication long-chain fatty acid transport|long-chain fatty-acyl-CoA metabolic process long-chain fatty acid-CoA ligase activity mitochondrial outer membrane|mitochondrion|lipid particle|plasma membrane YGL209W MIG2 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|MITOCHONDRION 0.0144388431871193 0.403189150648178 1179 Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter by glucose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding mitochondrion|nucleus YIL161W_p YIL161W hom 0.0144137901428015 0.404216208173927 1180 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene biological_process molecular_function cytoplasm YER184C_p YER184C hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0144128807978634 0.404105543343205 1181 Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source biological_process sequence-specific DNA binding cellular_component YHR103W SBE22 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|GOLGI APPARATUS 0.0143930490320184 0.404682553860912 1182 Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth fungal-type cell wall organization molecular_function cytoplasm YOR162C YRR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0143811168508004 0.405072219211895 1183 Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter in response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YDL187C_d YDL187C hom 0.0143706623737835 0.405413811858906 1184 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR033C_d YKR033C hom 0.0143644956635082 0.405615385412311 1185 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Unknown Unknown Unknown YLR171W_d YLR171W hom 0.0143483918478726 0.406562543566929 1186 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR194C-A_d YMR194C-A hom 0.0143429227754678 0.406321017191082 1187 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL069W NUF2 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0143375573228303 0.406846907819178 1188 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton spindle microtubule|Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region|spindle pole body YER104W RTT105 het FT NUCLEUS 0.0143297617546263 0.406751863080485 1189 Protein with a role in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated molecular_function nucleus|cytoplasm YPR052C NHP6A hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0143082120548975 0.4074579130579 1190 High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress mismatch repair|DNA replication-independent nucleosome organization|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly|chromatin remodeling nucleosome binding|sequence-specific DNA binding|DNA binding, bending MutSalpha complex|nucleus YMR227C TAF7 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0142834809820325 0.408269096272938 1191 TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YBR103W SIF2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0142786900126439 0.408426351946132 1192 WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus negative regulation of meiosis|negative regulation of chromatin silencing at telomere|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|nucleus|Set3 complex YKR066C CCP1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0142601869754053 0.409034022073733 1193 Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress cellular response to oxidative stress cytochrome-c peroxidase activity mitochondrion|mitochondrial intermembrane space YOR368W RAD17 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0142583305428327 0.409304766884507 1194 Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins double-strand break repair|reciprocal meiotic recombination|DNA damage checkpoint double-stranded DNA binding nucleus|checkpoint clamp complex YBR285W_p YBR285W hom 0.0142344715956475 0.409879449893251 1195 Putative protein of unknown function; YBR285W is not an essential gene biological_process molecular_function cellular_component YGR116W SPT6 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0142319258110933 0.40996320244899 1196 Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region poly(A)+ mRNA export from nucleus|regulation of transcription by chromatin organization|regulation of mRNA 3'-end processing|transcription antitermination|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|regulation of transcriptional start site selection at RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by glucose|negative regulation of transcription from RNA polymerase II promoter|nucleosome organization|chromatin maintenance|regulation of nucleosome density|nucleosome assembly|chromatin remodeling|regulation of histone H3-K36 methylation|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to stress core RNA polymerase II binding transcription factor activity|nucleosome binding|histone binding|DNA binding transcription antitermination factor activity mitochondrion|nucleus|transcriptionally active chromatin YOR055W_d YOR055W hom 0.0142082443213481 0.41074277567084 1197 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR233C EST1 hom FT NUCLEUS|CHROMOSOME 0.0141945611566299 0.411193613958178 1198 TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA telomere maintenance via telomerase|telomere maintenance|G-quadruplex DNA formation single-stranded DNA binding|RNA binding|telomeric DNA binding nucleolus|nucleus|telomerase holoenzyme complex YBR078W ECM33 hom FF|FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0141870090304963 0.411442570080881 1199 GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p fungal-type cell wall organization molecular_function mitochondrion|fungal-type cell wall|plasma membrane YDR438W THI74 hom FT MITOCHONDRION 0.0141850893656624 0.411505866154042 1200 Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication biological_process molecular_function mitochondrion|integral to membrane YNL258C DSL1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0141662977467655 0.412195528994255 1201 Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene retrograde vesicle-mediated transport, Golgi to ER|ER-dependent peroxisome organization molecular_function Dsl1p complex|endoplasmic reticulum|peroxisome|nucleus YMR074C YMR074C hom FT NUCLEUS 0.0141528629256782 0.412569313277961 1202 Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress apoptotic process molecular_function nucleus|cytoplasm YLR361C-A_p YLR361C-A hom 0.0141443291900078 0.412851192248655 1203 Putative protein of unknown function biological_process molecular_function cellular_component YGR129W SYF2 hom FT RNA PROCESSING|NUCLEUS 0.0141407989168884 0.412967834518453 1204 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest mRNA splicing, via spliceosome first spliceosomal transesterification activity|second spliceosomal transesterification activity U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YGL176C_p YGL176C hom 0.0141122528478646 0.414120773978171 1205 Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype biological_process molecular_function cellular_component YOR059C YOR059C hom FT LIPID METABOLISM 0.014096527829142 0.414432232995971 1206 Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress biological_process molecular_function lipid particle YOL123W HRP1 het FT RNA PROCESSING|NUCLEUS 0.014085961533121 0.414782198034522 1207 Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences mRNA cleavage|mRNA polyadenylation mRNA binding|RNA binding mRNA cleavage factor complex|nucleus|cytoplasmic stress granule|cytoplasm YPR180W AOS1 het FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0140835605553259 0.416257373810063 1208 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity nucleus|SUMO activating enzyme complex YOL079W_d YOL079W hom 0.0140744145911514 0.415164842555733 1209 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR279C RFM1 hom FT GENE SILENCING|NUCLEUS 0.0140596617718372 0.415654027229071 1210 DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance chromatin silencing|chromatin silencing at silent mating-type cassette unfolded protein binding histone deacetylase complex|nucleus YOR346W REV1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0140324255707616 0.416558038355879 1211 Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress error-prone translesion synthesis|error-free translesion synthesis deoxycytidyl transferase activity|DNA-directed DNA polymerase activity nuclear chromatin|mitochondrion|replication fork YER056C-A RPL34A hom FT TRANSLATION|RIBOSOME 0.0140249977923235 0.416804778739953 1212 Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YKL045W PRI2 het FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME 0.0139903570697363 0.417956632355396 1213 Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA synthesis involved in DNA repair|DNA replication|DNA replication initiation single-stranded DNA binding|DNA primase activity nuclear envelope|alpha DNA polymerase:primase complex|nucleus YDR164C SEC1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0139659494131218 0.418769346126207 1214 Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function positive regulation of vesicle fusion|exocytosis|vesicle docking involved in exocytosis SNARE binding plasma membrane|cellular bud neck|cellular bud tip YHR124W NDT80 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.0139565366467878 0.419083015833023 1215 Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) meiosis|transcription, DNA-dependent sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nuclear chromosome YNL090W RHO2 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0139232543267049 0.420193218423346 1216 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly microtubule-based process|fungal-type cell wall organization|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction GTPase activity membrane YGR128C UTP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.013898885086636 0.421007200353452 1217 Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|tRNA export from nucleus snoRNA binding|tRNA binding small-subunit processome|t-UTP complex|nucleolus|rDNA heterochromatin|90S preribosome YDL088C ASM4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0138738175252909 0.42184547202841 1218 FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication nuclear pore organization|mRNA export from nucleus in response to heat stress|mitotic cell cycle spindle assembly checkpoint nucleocytoplasmic transporter activity|phospholipid binding|single-stranded DNA binding nuclear pore central transport channel|integral to membrane|nuclear pore YPL229W_p YPL229W hom 0.0138564223284552 0.422427750526555 1219 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication biological_process molecular_function cytoplasm YDR515W SLF1 hom FT TRANSLATION|ION HOMEOSTASIS|ION HOMEOSTASIS 0.013844813813385 0.422816590151973 1220 RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cellular copper ion homeostasis|regulation of translation RNA binding polysome|cytoplasm YOR062C_p YOR062C hom FT NUCLEUS 0.0138280384124537 0.423378870161432 1221 Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YHR121W LSM12 hom FT NUCLEUS 0.0138180399854919 0.423714206658032 1222 Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress RNA metabolic process RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YKL090W CUE2 hom 0.013811621652648 0.423929552475714 1223 Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination biological_process ubiquitin binding cellular_component YDR460W TFB3 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0137996768239662 0.424399615985411 1224 Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity holo TFIIH complex|TFIIK complex YHL030W ECM29 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.0137824080749033 0.424910525029593 1225 Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress proteasome assembly protein complex scaffold proteasome complex|nucleus|cytoplasm YHL044W_p YHL044W hom FT PLASMA MEMBRANE 0.0137764785073886 0.425109797682937 1226 Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern biological_process molecular_function plasma membrane YDR140W MTQ2 hom FT TRANSLATION|NUCLEUS 0.0137672235879516 0.425420933149394 1227 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC peptidyl-glutamine methylation|regulation of translation protein methyltransferase activity|S-adenosylmethionine-dependent methyltransferase activity eRF1 methyltransferase complex|nucleus|cytoplasm YPL238C_d YPL238C het FF 0.0137617058862454 0.425813708082707 1228 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Unknown Unknown Unknown YMR114C YMR114C hom FT NUCLEUS 0.013751239786981 0.427134497617058 1229 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene biological_process molecular_function ribosome|nucleus|cytoplasm YOR135C_d IRC14 hom 0.0137350624322058 0.426503172162575 1230 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YBR102C EXO84 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|SITE OF POLARIZED GROWTH 0.0137309783996314 0.426640716639141 1231 Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate spliceosomal complex assembly|Golgi to plasma membrane transport|exocyst localization|exocytosis|exocyst assembly molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip|exocyst YNL044W YIP3 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0137221498458514 0.427214167152358 1232 Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus|endoplasmic reticulum|ER to Golgi transport vesicle YPR006C ICL2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0137181136365363 0.427281126692614 1233 2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol threonine catabolic process|propionate metabolic process methylisocitrate lyase activity mitochondrion|mitochondrial matrix YLR314C CDC3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0137007689609809 0.427934800157279 1234 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells septin ring disassembly|maintenance of cell polarity|cell cycle cytokinesis|septin ring assembly structural molecule activity|GTP binding|1-phosphatidylinositol binding mating projection base|septin filament array|cellular bud neck septin ring|septin complex|prospore membrane|ascospore wall YHL026C_p YHL026C hom 0.0136963515652001 0.427876872039328 1235 Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) biological_process molecular_function cellular_component YDR200C VPS64 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0136954857222376 0.427837148175366 1236 Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance re-entry into mitotic cell cycle after pheromone arrest|protein targeting to vacuole molecular_function endoplasmic reticulum membrane|cytoplasm YOR356W CIR2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0136944246342486 0.428148768807066 1237 Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response biological_process molecular_function mitochondrion YDR370C DXO1 hom 0.0136919530207087 0.427956339641562 1238 Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p biological_process molecular_function cytoplasm YPL198W RPL7B hom FT TRANSLATION|NUCLEUS|RIBOSOME 0.0136577830274611 0.429110213850407 1239 Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|nucleolus|cytoplasm YMR209C_p YMR209C hom 0.0136504259990419 0.429358886999177 1240 Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YNL198C_d YNL198C hom 0.0136327265992467 0.429957482985497 1241 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL043W NOP4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0136266718919341 0.430162365118089 1242 Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) rRNA processing RNA binding nucleolus YER015W FAA2 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0136262503524635 0.430176631485151 1243 Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome long-chain fatty acid metabolic process long-chain fatty acid-CoA ligase activity|very long-chain fatty acid-CoA ligase activity|medium-chain fatty acid-CoA ligase activity mitochondrion|peroxisome YGR007W ECT1 hom FT LIPID METABOLISM|NUCLEUS 0.0136253105606906 0.430208438307495 1244 Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases phosphatidylethanolamine biosynthetic process ethanolamine-phosphate cytidylyltransferase activity nucleus|cytoplasm YML007C-A_p YML007C-A hom FF|FT MITOCHONDRION 0.0136124949730128 0.430642312182683 1245 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria biological_process molecular_function mitochondrion YNL219C ALG9 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0136095536823658 0.430741925867848 1246 Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation mannosyltransferase activity integral to membrane|endoplasmic reticulum YKR020W VPS51 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL DIVISION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0136018242265223 0.431141292014943 1247 Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p vesicle organization|retrograde transport, endosome to Golgi|budding cell apical bud growth|regulation of cell size|protein targeting to vacuole protein binding Golgi apparatus|GARP complex YAR020C PAU7 hom 0.0135557266439923 0.432567262956313 1248 Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component YLR096W KIN2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.0135499328975169 0.43276400206421 1249 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p protein autophosphorylation|exocytosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity plasma membrane YOL041C NOP12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0135381302555134 0.433370890061846 1250 Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA binding nucleolus|preribosome YFL017W-A SMX2 het FT RNA PROCESSING|NUCLEUS 0.0135347443559636 0.433280007290129 1251 Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YPR172W_p YPR172W hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|NUCLEUS 0.0135001038180984 0.434458191366617 1252 Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YMR169C ALD3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0134948950557509 0.434635510140603 1253 Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose beta-alanine biosynthetic process|polyamine catabolic process aldehyde dehydrogenase (NAD) activity cytoplasm YDL238C GUD1 hom 0.0134889550460325 0.434837773304128 1254 Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures guanine metabolic process guanine deaminase activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides cytoplasm YKL113C RAD27 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0134879781624082 0.434871042351132 1255 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family double-strand break repair via nonhomologous end joining|replicative cell aging|DNA replication, removal of RNA primer|base-excision repair, base-free sugar-phosphate removal|gene conversion at mating-type locus, DNA repair synthesis 5'-3' exonuclease activity|5'-flap endonuclease activity mitochondrion|nucleus YGL123W RPS2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|NUCLEUS|RIBOSOME 0.01348173346778 0.435152268513254 1256 Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 positive regulation of translational fidelity|rRNA export from nucleus structural constituent of ribosome|SSU rRNA binding small-subunit processome|cytosolic small ribosomal subunit YKL089W MIF2 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0134649040880046 0.435657287058829 1257 Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 chromosome segregation|mitotic spindle organization in nucleus centromeric DNA binding condensed nuclear chromosome, centromeric region|condensed nuclear chromosome inner kinetochore YER060W FCY21 hom 0.0134381590566181 0.436569643589253 1258 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YAL008W FUN14 hom FT MITOCHONDRION 0.0134322258285806 0.436772193547203 1259 Mitochondrial protein of unknown function biological_process molecular_function mitochondrial outer membrane|mitochondrion YKR076W ECM4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS 0.0134319508718662 0.436781581396863 1260 Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization|glutathione metabolic process glutathione transferase activity cytoplasm YBR067C TIP1 hom FT CELL WALL ORG/BIOGENESIS 0.0134086092169175 0.43757895917479 1261 Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins fungal-type cell wall organization structural constituent of cell wall|lipase activity fungal-type cell wall YER006W NUG1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.013404784638742 0.437709690951126 1262 GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus ribosomal large subunit export from nucleus|rRNA processing RNA binding|GTPase activity nucleolus|nucleus|preribosome, large subunit precursor YNL131W TOM22 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0133860960241351 0.438348828348068 1263 Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes protein import into mitochondrial matrix|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YDR022C ATG31 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0133723803855287 0.438818234508533 1264 Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion autophagy|mitochondrion degradation|piecemeal microautophagy of nucleus molecular_function pre-autophagosomal structure|cytoplasm YGL034C_d YGL034C hom 0.0133714234956869 0.438850994025468 1265 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR009W RLP24 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0133607528136373 0.439626303869778 1266 Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp ribosomal large subunit biogenesis molecular_function nucleolus|preribosome, large subunit precursor YLR367W RPS22B hom FT TRANSLATION|RIBOSOME 0.0133506636033263 0.439562063163049 1267 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YER119C-A_d YER119C-A hom FF 0.0133433968717086 0.439811119828796 1268 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Unknown Unknown Unknown YBL079W NUP170 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0133248649960713 0.440651293386254 1269 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication nucleocytoplasmic transport|chromosome segregation|protein targeting to nuclear inner membrane|mitotic cell cycle spindle assembly checkpoint|nuclear pore complex assembly structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YJL059W YHC3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.0133209340084444 0.440581513475847 1270 Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) basic amino acid transport|regulation of intracellular pH|arginine transport molecular_function integral to membrane|fungal-type vacuole YKR090W PXL1 hom FT SIGNALING|SITE OF POLARIZED GROWTH 0.0133205661692325 0.440594135441113 1271 Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress maintenance of cell polarity|regulation of Rho protein signal transduction Rho GDP-dissociation inhibitor activity incipient cellular bud site|cellular bud|mating projection tip|cellular bud neck|cytoplasm|cellular bud tip YGL105W ARC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0133181325874036 0.440677646128905 1272 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress positive regulation of ligase activity|tRNA aminoacylation for protein translation enzyme activator activity|tRNA binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding methionyl glutamyl tRNA synthetase complex|cytoplasm YGR060W ERG25 het FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0133111636627571 0.440916841612832 1273 C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol ergosterol biosynthetic process C-4 methylsterol oxidase activity plasma membrane|endoplasmic reticulum membrane YPL105C SYH1 hom FT PROTEIN LOCALIZATION|MITOCHONDRION 0.0132799389390974 0.441989485391776 1274 Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication nuclear pore distribution molecular_function mitochondrion|ribosome|cytoplasmic mRNA processing body|extrinsic to membrane|cytoplasm YMR278W PGM3 hom FT CARBOHYDRATE METABOLISM|NUCLEUS 0.0132785224926792 0.44203817907755 1275 Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential biological_process phosphoglucomutase activity nucleus|cytoplasm YGL261C_p PAU11 hom 0.0132773387307918 0.442146978704309 1276 Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 biological_process molecular_function cellular_component YNL130C-A_p DGR1 hom FT MITOCHONDRION 0.0132753563142983 0.442147035021688 1277 Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose biological_process molecular_function cellular_component YBR236C ABD1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|RNA POL II, HOLOENZYME 0.0132621792878363 0.442600237221532 1278 Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA 7-methylguanosine mRNA capping|transcription from RNA polymerase II promoter mRNA (guanine-N7-)-methyltransferase activity nucleus|DNA-directed RNA polymerase II, holoenzyme YDR223W CRF1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0132603717392222 0.442662425595537 1279 Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter transcription corepressor activity nuclear chromatin|nucleus|cytoplasm YMR199W CLN1 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0132397229604292 0.443441220658179 1280 G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity nucleus|cytoplasm YKL071W_p YKL071W hom FT OXIDATION-REDUCTION PROCESS 0.013239108410676 0.443394361340121 1281 Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function integral to membrane|cytoplasm YBR257W POP4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.013202136531893 0.44466866808604 1282 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YPL106C SSE1 hom 0.0131866203105435 0.445204085922412 1283 ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication protein folding|protein refolding peptide binding|adenyl-nucleotide exchange factor activity|ATP binding polysome|cytoplasm YIL066C RNR3 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0131471674369518 0.446567138536744 1284 Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor mitochondrion|ribonucleoside-diphosphate reductase complex|cytoplasm YGR100W MDR1 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM 0.0131416147890526 0.446759166700786 1285 Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function intracellular protein transport Rab GTPase activator activity cytoplasm YBR065C ECM2 hom FT RNA PROCESSING|NUCLEUS 0.0131336658012509 0.447034149616949 1286 Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p mRNA splicing, via spliceosome molecular_function nucleus YPR013C_p CMR3 hom 0.0131177017693674 0.44785782909724 1287 Putative zinc finger protein; YPR013C is not an essential gene biological_process sequence-specific DNA binding cellular_component YMR317W_p YMR317W hom 0.0131147349631821 0.447757174036568 1288 Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene biological_process molecular_function cellular_component YKL183W LOT5 hom FT NUCLEUS 0.0130809331431645 0.448928468850306 1289 Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YGR152C RSR1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0130778432711368 0.448967952232366 1290 GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases bipolar cellular bud site selection|small GTPase mediated signal transduction|axial cellular bud site selection|cellular bud site selection GTPase activity|signal transducer activity plasma membrane|cellular bud neck YOR226C ISU2 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.013027974862689 0.450699497762235 1291 Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved cellular iron ion homeostasis|iron-sulfur cluster assembly|tRNA wobble uridine modification molecular_function mitochondrial matrix YGR192C TDH3 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0130126267884279 0.451233177773163 1292 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication gluconeogenesis|reactive oxygen species metabolic process|glycolysis|apoptotic process glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YGR147C NAT2 het FT MITOCHONDRION 0.0129976624088938 0.451753859991235 1293 Protein with an apparent role in acetylation of N-terminal methionine residues N-terminal peptidyl-methionine acetylation molecular_function mitochondrion YEL017W GTT3 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.0129873285570033 0.452113622187371 1294 Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery biological_process molecular_function cellular_component YOR297C TIM18 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0129785532535889 0.45241925220447 1295 Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex cellular response to oxidative stress|protein import into mitochondrial inner membrane|apoptotic process|response to acid|response to osmotic stress|response to arsenic-containing substance protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YAL067W-A_p YAL067W-A hom 0.0129680875945453 0.452851334370718 1296 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YCL046W_d YCL046W hom 0.0129675139686171 0.453141185725138 1297 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Unknown Unknown Unknown YDR418W RPL12B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0129435460950666 0.453639657020553 1298 Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YKL195W MIA40 het FF|FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.012938942689411 0.454137238164754 1299 Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial outer membrane translocase complex|mitochondrial intermembrane space YER027C GAL83 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0129273916864666 0.454203450590732 1300 One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain signal transduction|cell adhesion|protein phosphorylation|invasive growth in response to glucose limitation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|cytoplasm YHR025W THR1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0129162456326042 0.454592681208929 1301 Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway threonine biosynthetic process|methionine metabolic process|isoleucine metabolic process|homoserine metabolic process homoserine kinase activity cellular_component YPR152C URN1 hom FT RNA PROCESSING|NUCLEUS 0.012907287503414 0.454905643574826 1302 Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase biological_process molecular_function nucleus YLR381W CTF3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0129001078496798 0.455156560437194 1303 Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 chromosome segregation|DNA replication initiation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YML009W-B_d YML009W-B hom 0.0128842865717319 0.455709762443598 1304 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YGL088W_d YGL088W hom 0.0128777242542891 0.455939329355156 1305 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Unknown Unknown Unknown YHR132W-A IGO2 hom FT NUCLEUS 0.0128673562564743 0.456302161341598 1306 Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p mRNA stabilization molecular_function nucleus|cytoplasm YKL133C_p YKL133C hom 0.0128542969074823 0.456759409017651 1307 Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) biological_process molecular_function cellular_component YLR043C TRX1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.0128470678471022 0.457012631113777 1308 Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress vacuole inheritance|regulation of DNA replication|cell redox homeostasis|protein deglutathionylation|retrograde vesicle-mediated transport, Golgi to ER|cellular response to oxidative stress|ER to Golgi vesicle-mediated transport|vacuole fusion, non-autophagic disulfide oxidoreductase activity cytosol|fungal-type vacuole YER076C_p YER076C hom FT MITOCHONDRION 0.0128450649280083 0.45708280401881 1309 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization biological_process molecular_function mitochondrion YJL089W SIP4 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0128409607041305 0.457226615700279 1310 C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YDL178W DLD2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0128373683556046 0.457352512152897 1311 D-lactate dehydrogenase, located in the mitochondrial matrix lactate metabolic process actin binding|D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial matrix YBR036C CSG2 hom FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0128350319800494 0.457434402565224 1312 Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress cellular calcium ion homeostasis|glycosphingolipid biosynthetic process enzyme regulator activity integral to endoplasmic reticulum membrane|integral to membrane YER004W FMP52 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0128253026866146 0.457775504639317 1313 Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function mitochondrial outer membrane|mitochondrion|endoplasmic reticulum YLL001W DNM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|PEROXISOME ORGANIZATION|MITOCHONDRION 0.0128016502509086 0.458605336917603 1314 Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance mitochondrion inheritance|peroxisome fission|mitochondrial fission|chronological cell aging|peroxisome organization|mitochondrion organization|protein homooligomerization protein homodimerization activity|GTPase activity|identical protein binding mitochondrial outer membrane|mitochondrion|peroxisome YIR012W SQT1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RIBOSOME 0.0127760805618523 0.459503382405053 1315 Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress ribosomal large subunit assembly molecular_function cytosol|cytosolic ribosome|cytoplasm YER146W LSM5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0127723375724464 0.459634924299686 1316 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YDL048C STP4 hom FT NUCLEUS|MITOCHONDRION 0.0127699373388036 0.459719288093643 1317 Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress biological_process sequence-specific DNA binding mitochondrion|nucleus|cytoplasm YIR015W RPR2 het FT RNA PROCESSING|NUCLEUS 0.0127671412642777 0.459817575891421 1318 Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress tRNA processing|intronic box C/D snoRNA processing ribonuclease P activity nucleolar ribonuclease P complex YEL040W UTR2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0127182419230947 0.461538388433695 1319 Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck cell wall chitin metabolic process|fungal-type cell wall organization transferase activity, transferring glycosyl groups cellular bud neck septin ring|fungal-type cell wall YNL128W TEP1 hom FT CELL WALL ORG/BIOGENESIS 0.0127175102626712 0.461631004788075 1320 PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 ascospore wall assembly|phosphatidylinositol dephosphorylation molecular_function cytoplasm YHR097C_p YHR097C hom FT NUCLEUS 0.0127100898945571 0.461825615026609 1321 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YLR406C-A_p YLR406C-A hom 0.0126999021789602 0.462184706716127 1322 Putative protein of unknown function biological_process molecular_function cellular_component YCL058C FYV5 hom FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0126961789440741 0.462382770165222 1323 Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis pheromone-dependent signal transduction involved in conjugation with cellular fusion|cellular ion homeostasis molecular_function integral to membrane YDL170W UGA3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0126887619327471 0.46257755096189 1324 Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus nitrogen utilization|nitrogen catabolite activation of transcription from RNA polymerase II promoter|gamma-aminobutyric acid catabolic process sequence-specific DNA binding transcription factor recruiting transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YMR206W_p YMR206W hom 0.0126789072474424 0.46292521694077 1325 Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication biological_process molecular_function cellular_component YJL178C ATG27 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0126739600855707 0.463433636920967 1326 Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site vesicle organization|peroxisome degradation|protein localization to pre-autophagosomal structure|CVT pathway|autophagic vacuole assembly phosphatidylinositol-3-phosphate binding trans-Golgi network|mitochondrion|pre-autophagosomal structure|membrane YOR052C YOR052C hom FT NUCLEUS 0.0126706786570785 0.4632156270268 1327 Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YJL217W REE1 hom 0.0126698283719004 0.46324564176576 1328 Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle biological_process molecular_function cytoplasm YHR136C SPL2 hom 0.0126673592988585 0.463332805232818 1329 Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm response to temperature stimulus enzyme inhibitor activity cytoplasm YPR108W-A_p YPR108W-A hom 0.012621459525153 0.46515469443508 1330 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YDL209C CWC2 het FT CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0125768809416452 0.467265481694156 1331 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 mRNA cis splicing, via spliceosome|mRNA splicing, via spliceosome|spliceosomal snRNP assembly pre-mRNA binding|first spliceosomal transesterification activity|U6 snRNA binding|second spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome|U2-type catalytic step 2 spliceosome YPL006W NCR1 hom FT LIPID METABOLISM 0.0125747805295858 0.466674107615157 1332 Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein sphingolipid metabolic process sphingolipid transporter activity fungal-type vacuole membrane|integral to membrane YMR085W_p YMR085W hom FT CARBOHYDRATE METABOLISM 0.0125636195960187 0.467202717597337 1333 Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component YLR262C-A TMA7 hom FT TRANSLATION|NUCLEUS 0.0125463248411832 0.46768317986618 1334 Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress cytoplasmic translation molecular_function nucleus|cytoplasm YER023W PRO3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0125410461608746 0.467804092981673 1335 Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis proline biosynthetic process pyrroline-5-carboxylate reductase activity cytoplasm YCL061C MRC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0125364907779626 0.467965791642508 1336 S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres maintenance of DNA repeat elements|intra-S DNA damage checkpoint|DNA repair|DNA replication checkpoint|telomere maintenance|replication fork protection|DNA replication|mitotic sister chromatid cohesion|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere molecular_function nuclear chromosome|replication fork protection complex|nucleus|nuclear replication fork YBR031W RPL4A hom FT TRANSLATION|RIBOSOME 0.0125090308308103 0.46894116994368 1337 Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR287C-A RPS30A hom FT TRANSLATION|RIBOSOME 0.012483994160619 0.469831452289272 1338 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasmic stress granule YPL171C OYE3 hom FT OXIDATION-REDUCTION PROCESS 0.0124797075006967 0.469983975763829 1339 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death apoptotic process NADPH dehydrogenase activity cellular_component YGL237C HAP2 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0124749320168196 0.470352692786862 1340 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex|nucleus YKL047W_p ANR2 hom 0.0124661036980056 0.470468193160702 1341 Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YJR117W STE24 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0124598748817373 0.470689995178925 1342 Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans CAAX-box protein processing|peptide pheromone maturation metalloendopeptidase activity mitochondrial outer membrane|integral to endoplasmic reticulum membrane|integral to membrane|nuclear inner membrane YCL038C ATG22 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.0124564221777456 0.470812967420897 1343 Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation amino acid export from vacuole molecular_function integral to fungal-type vacuolar membrane|integral to membrane YGL251C HFM1 hom FT CELL CYCLE|NUCLEUS 0.0124449827748348 0.471220522245869 1344 Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity meiosis|synapsis|DNA unwinding involved in replication|reciprocal meiotic recombination DNA helicase activity nucleus YER175W-A_p YER175W-A hom 0.0124112915788367 0.472421977303298 1345 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YLR277C YSH1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0123814954104844 0.473485936218121 1346 Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication termination of RNA polymerase II transcription|mRNA cleavage|mRNA polyadenylation|RNA splicing|snoRNA splicing|snoRNA 3'-end processing endoribonuclease activity|RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YOL054W PSH1 hom FT PROTEOLYSIS|NUCLEUS|CHROMOSOME 0.0123522312356658 0.474532179677008 1347 E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity chromosome, centromeric region|nucleus YLR081W GAL2 hom FT CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0123520659701002 0.47453809180066 1348 Galactose permease, required for utilization of galactose; also able to transport glucose galactose transport|galactose metabolic process galactose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YJR048W CYC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0123332181690085 0.475212608359404 1349 Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen|mitochondrial electron transport, ubiquinol to cytochrome c electron carrier activity mitochondrion|mitochondrial intermembrane space YBL104C SEA4 hom 0.0123225058139291 0.475596211524676 1350 Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites biological_process molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane|cytoplasm YBR073W RDH54 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS 0.0122824846674251 0.477030846034816 1351 DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p DNA geometric change|heteroduplex formation|meiotic sister chromatid segregation|reciprocal meiotic recombination|double-strand break repair via break-induced replication DNA-dependent ATPase activity|DNA translocase activity nucleus YDR162C NBP2 hom FF|FT PROTEIN PHOSPHORYLATION|NUCLEUS 0.0122809531869486 0.477677965392677 1352 Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) negative regulation of protein kinase activity|response to heat|hyperosmotic response molecular_function nucleus|cytoplasm YMR315W YMR315W hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0122748471714439 0.477304895497266 1353 Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress NADPH regeneration oxidoreductase activity nucleus|cytoplasm YCL052C PBN1 het FT LIPID METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0122599636002527 0.477839196760513 1354 Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X GPI anchor biosynthetic process|ER-associated protein catabolic process|protein processing mannosyltransferase activity endoplasmic reticulum YNR028W CPR8 hom 0.0122385962244888 0.478606829589224 1355 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity fungal-type vacuole YNL292W PUS4 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0122238220937254 0.479137990556355 1356 Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) tRNA modification pseudouridine synthase activity mitochondrion|nucleus YDR256C CTA1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0122179166074347 0.479350395114552 1357 Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation response to reactive oxygen species|age-dependent response to reactive oxygen species|hydrogen peroxide catabolic process catalase activity peroxisomal matrix|mitochondrial matrix YBL072C RPS8A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0122050039839421 0.479815006559957 1358 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YLR380W CSR1 hom FT KETONE METABOLISM|LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0121970928848459 0.480099778486108 1359 Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress phosphatidylinositol metabolic process|Golgi to plasma membrane protein transport|Golgi to plasma membrane transport|Unknown|negative regulation of fatty acid biosynthetic process|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cytosol|endosome|mitochondrion|lipid particle YGR015C_p YGR015C hom FT MITOCHONDRION 0.0121511020133788 0.481757111867157 1360 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion YGR261C APL6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0121413915703409 0.482107436070455 1361 Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools vesicle-mediated transport|Golgi to vacuole transport molecular_function AP-3 adaptor complex YAL042W ERV46 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0121146430198906 0.483073160632562 1362 Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YPR171W BSP1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0120975918086687 0.484080999152744 1363 Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton actin cortical patch localization protein binding, bridging mating projection tip|membrane|cellular bud neck|actin cortical patch|cellular bud tip YKL208W CBT1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0120775939142736 0.484934418348532 1364 Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p cytochrome complex assembly|mRNA processing|mRNA stabilization molecular_function mitochondrion YLR109W AHP1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0120674102895677 0.484976526716176 1365 Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p cellular response to oxidative stress|response to metal ion|cell redox homeostasis thioredoxin peroxidase activity plasma membrane|cytoplasm YOL097W-A_p YOL097W-A hom 0.0120633027527447 0.484929677999509 1366 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YHL008C_p YHL008C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0120364274301187 0.48609832149349 1367 Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole chloride transport|monocarboxylic acid transport monocarboxylic acid transmembrane transporter activity|transporter activity integral to membrane|fungal-type vacuole YDR024W_d FYV1 hom 0.0120247150208004 0.486587841925356 1368 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Unknown Unknown Unknown YDL168W SFA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0120212233938903 0.486519219143735 1369 Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress furaldehyde metabolic process|formaldehyde catabolic process|amino acid catabolic process to alcohol via Ehrlich pathway S-(hydroxymethyl)glutathione dehydrogenase activity|hydroxymethylfurfural reductase (NADH) activity|alcohol dehydrogenase (NAD) activity mitochondrion|cytoplasm YCR025C_d YCR025C hom 0.0120009313573075 0.487190279916323 1370 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Unknown Unknown Unknown YKR048C NAP1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME 0.0119989960922489 0.487260512944289 1371 Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress budding cell bud growth|M phase of mitotic cell cycle|nucleosome disassembly|protein import into nucleus|nucleosome assembly cyclin binding|histone binding nucleus|nuclear nucleosome|cytoplasm YJL139C YUR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0119969810032376 0.487333648683916 1372 Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication cell wall mannoprotein biosynthetic process|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YLR455W YLR455W hom FT NUCLEUS 0.0119836955886907 0.487815978428644 1373 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YFR006W_p YFR006W hom 0.0119779926621651 0.488023103321028 1374 Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene biological_process molecular_function cytoplasm YIR021W MRS1 hom FT RNA PROCESSING|MITOCHONDRION 0.0119779026551437 0.488351068089724 1375 Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Group I intron splicing endodeoxyribonuclease activity|RNA binding mitochondrion YDL030W PRP9 het FT RNA PROCESSING|NUCLEUS 0.0119692481822181 0.488535487662765 1376 Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YOR210W RPB10 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0119643335451808 0.488519382257482 1377 RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase II, core complex YHR139C-A_d YHR139C-A hom 0.0119573849483462 0.48877195108273 1378 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL079W SMY1 hom FT VESICLE-MEDIATED TRANSPORT|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0119537312108699 0.488904785988797 1379 Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins exocytosis motor activity incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip YBL047C EDE1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0119301096183245 0.489764039156345 1380 Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death endoplasmic reticulum unfolded protein response|endocytosis ubiquitin binding cellular bud neck|mating projection tip|actin cortical patch|cellular bud tip YJL153C INO1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM 0.011928959903357 0.490259585704892 1381 Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element inositol biosynthetic process inositol-3-phosphate synthase activity cytoplasm YER014W HEM14 hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0119061906457908 0.490634936815633 1382 Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides heme biosynthetic process oxygen-dependent protoporphyrinogen oxidase activity mitochondrion|mitochondrial inner membrane YAR002C-A ERP1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0118837259347114 0.491776998412946 1383 Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|ER to Golgi transport vesicle YMR193C-A_d YMR193C-A hom 0.0118637877564604 0.492180882714708 1384 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER114C BOI2 hom FT SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0118355327501779 0.493212466109911 1385 Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication cytokinesis, completion of separation|establishment of cell polarity|budding cell apical bud growth|Rho protein signal transduction phospholipid binding site of polarized growth|cellular bud|cellular bud neck YGL044C RNA15 het FT RNA PROCESSING|NUCLEUS 0.0118310299686644 0.493376968438237 1386 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation mRNA binding|protein heterodimerization activity mRNA cleavage factor complex YAR030C_d YAR030C hom 0.0118044962281929 0.494346934760321 1387 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Unknown Unknown Unknown YKL109W HAP4 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0118044350104688 0.494349173810956 1388 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex regulation of cellular respiration|carbon catabolite activation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity CCAAT-binding factor complex YDR287W INM2 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM 0.0118012709634102 0.494916036917774 1389 Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy inositol phosphate dephosphorylation inositol monophosphate 1-phosphatase activity cellular_component YNL146C-A_p YNL146C-A hom 0.0117949129779403 0.494697510066851 1390 Putative protein of unknown function biological_process molecular_function cellular_component YOR115C TRS33 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0117646551099691 0.495805277337493 1391 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport|protein complex assembly molecular_function trans-Golgi network|TRAPP complex|cis-Golgi network YKR001C VPS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|PEROXISOME ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.0117464822194274 0.497049944628004 1392 Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis vacuolar transport|peroxisome fission|endocytosis|actin cytoskeleton organization|protein retention in Golgi apparatus|peroxisome organization|lipid tube assembly|protein targeting to vacuole GTPase activity mitochondrial outer membrane|fungal-type vacuole membrane|peroxisome|actin cortical patch YNL032W SIW14 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0117423895274743 0.496621284407363 1393 Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm actin filament organization|endocytosis|protein dephosphorylation|response to stress protein tyrosine phosphatase activity cytoplasm YDR141C DOP1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0117299805061039 0.497076370637135 1394 Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies endoplasmic reticulum organization|cell morphogenesis|Golgi to endosome transport molecular_function trans-Golgi network|endosome|mitochondrion|cytoplasm YER077C_p YER077C hom FT MITOCHONDRION 0.0117213076666587 0.49739456860352 1395 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function mitochondrion YNL321W VNX1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0117007600284092 0.498148873826522 1396 Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate potassium ion transport|sodium ion transport|hydrogen transport potassium:hydrogen antiporter activity|sodium:hydrogen antiporter activity|calcium:hydrogen antiporter activity fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum membrane|fungal-type vacuole YOL027C MDM38 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|TRANSLATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0116861558373142 0.498685364277391 1397 Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome potassium ion transport|mitochondrial respiratory chain complex III biogenesis|cellular potassium ion homeostasis|proton transport|positive regulation of mitochondrial translation|mitochondrial respiratory chain complex IV biogenesis|protein insertion into mitochondrial membrane ribosome binding mitochondrion|mitochondrial inner membrane YDR367W KEI1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0116391209710044 0.50047921802898 1398 Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene inositolphosphoceramide metabolic process inositol phosphoceramide synthase regulator activity integral to membrane|Golgi apparatus|integral to Golgi membrane|inositol phosphoceramide synthase complex|cytoplasm YDR019C GCV1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0116376890083105 0.500468009327041 1399 T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm glycine catabolic process|glycine metabolic process|one-carbon metabolic process glycine dehydrogenase (decarboxylating) activity mitochondrion|glycine cleavage complex YDL075W RPL31A hom FT TRANSLATION|RIBOSOME 0.0116310725330799 0.500711629834044 1400 Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YML024W RPS17A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0116258153709911 0.500905244833061 1401 Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YHR070W TRM5 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0116251977657433 0.50124755115958 1402 tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya tRNA methylation tRNA (guanine) methyltransferase activity nucleus|mitochondrial matrix|cytoplasm YLL037W_d YLL037W het 0.0116138149179802 0.501347355726721 1403 Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Unknown Unknown Unknown YEL024W RIP1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0116061641260712 0.502012494671803 1404 Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|mitochondrial respiratory chain complex III YMR299C DYN3 hom FT CYTOSKELETON 0.0116038990932712 0.501712822255417 1405 Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration nuclear migration along microtubule motor activity cytoplasmic dynein complex|cytoplasmic microtubule YIL011W TIR3 hom 0.0115997846816257 0.502055971461754 1406 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth biological_process molecular_function fungal-type cell wall YDR091C RLI1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM|NUCLEUS|RIBOSOME 0.0115722709107543 0.502879480184784 1407 Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species translational termination|ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|translational initiation ATPase activity|iron ion binding cytosolic ribosome|nucleus|cytoplasm YDR321W ASP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.011537530892196 0.504162573008341 1408 Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively asparagine catabolic process asparaginase activity intracellular YGR286C BIO2 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0115364076714909 0.504585981746421 1409 Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant biotin biosynthetic process biotin synthase activity mitochondrion YLL031C GPI13 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0114925974131741 0.505824709485918 1410 ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity, transferring phosphorus-containing groups integral to membrane|endoplasmic reticulum YML004C GLO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.011492361214521 0.505833454331906 1411 Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress methylglyoxal catabolic process to D-lactate|glutathione metabolic process lactoylglutathione lyase activity nucleus|cytoplasm YNL227C JJJ1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0114831958342449 0.506172848071471 1412 Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|endocytosis|regulation of cell size|rRNA processing ATPase activator activity cytosol|mitochondrion|cytosolic large ribosomal subunit|nucleolus|cytoplasm YLR416C_d YLR416C hom 0.0114539958257154 0.507254921352473 1413 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR005C TFG2 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0114520684471106 0.507326387488816 1414 TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 transcriptional start site selection at RNA polymerase II promoter|transcription initiation from RNA polymerase II promoter|promoter clearance from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|core RNA polymerase II recruiting transcription factor activity transcription factor TFIIF complex YLR079W SIC1 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0114457789239802 0.507559636301928 1415 Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 regulation of cyclin-dependent protein kinase activity|negative regulation of macroautophagy|G1/S transition of mitotic cell cycle cyclin-dependent protein kinase inhibitor activity nucleus|cytoplasm YDR263C DIN7 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0114376065783092 0.50824285988833 1416 Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination DNA repair nuclease activity mitochondrion YOR250C CLP1 het FT RNA PROCESSING|NUCLEUS 0.0114343357888775 0.507984152624842 1417 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation mRNA 3'-end processing|mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage factor complex YML081W TDA9 hom FT NUCLEUS 0.0114322454766058 0.508061718938044 1418 DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication biological_process sequence-specific DNA binding nucleus YMR320W_d YMR320W hom 0.0114296030966224 0.508159780004771 1419 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR134W BAG7 hom FT SIGNALING 0.0114278205832435 0.508225936263431 1420 Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p small GTPase mediated signal transduction Rho GTPase activator activity intracellular YCR095C_p OCA4 hom 0.0114238809670222 0.508372167323209 1421 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm YHR151C MTC6 hom 0.0114049491141276 0.509075189215818 1422 Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YLR056W ERG3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0113910873846534 0.509590258069591 1423 C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase ergosterol biosynthetic process C-5 sterol desaturase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum lumen YJL160C_p YJL160C hom 0.0113859731555283 0.509780359484479 1424 Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication biological_process molecular_function cellular_component YPR069C SPE3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0113843083490281 0.509842250141299 1425 Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells spermidine biosynthetic process|pantothenate biosynthetic process spermidine synthase activity nucleus|cytoplasm YMR036C MIH1 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.011374044850202 0.51022389159366 1426 Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity protein tyrosine phosphatase activity nucleus|cytoplasm YGL120C PRP43 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0113687126474066 0.510422224982656 1427 RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|spliceosomal complex disassembly|ribosomal large subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA|rRNA processing ATP-dependent RNA helicase activity mitochondrion|post-mRNA release spliceosomal complex|90S preribosome YLR460C_p YLR460C hom FT OXIDATION-REDUCTION PROCESS 0.0113450812545502 0.511301688731474 1428 Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine biological_process molecular_function cellular_component YAL065C_p YAL065C hom 0.011333447442971 0.511734941969892 1429 Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component YHR115C DMA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.011298368406953 0.513042474118467 1430 Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger establishment of mitotic spindle orientation|protein autoubiquitination|septin ring assembly|mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity cytoplasm YLR372W SUR4 hom FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0112749112733555 0.513917782283493 1431 Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis post-Golgi vesicle-mediated transport|fatty acid elongation|fatty acid biosynthetic process|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum YER150W SPI1 hom 0.0112701047902891 0.514348441025641 1432 GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p response to acid molecular_function fungal-type cell wall YDR013W PSF1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0112671877021866 0.514206158918316 1433 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YOR185C GSP2 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS 0.0112521151301851 0.514957381807951 1434 GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress nucleus organization GTPase activity integral to membrane|nucleus YLR050C_p YLR050C hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0112341871451524 0.515439254641171 1435 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YGL135W RPL1B hom FT TRANSLATION|RIBOSOME 0.0112302489394991 0.515586511677301 1436 Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YGR200C ELP2 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0112206685198814 0.515944832982381 1437 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin regulation of transcription from RNA polymerase II promoter|protein urmylation|tRNA wobble uridine modification molecular_function Elongator holoenzyme complex|nucleus|cytoplasm YNL179C_d YNL179C hom 0.0112021390998728 0.516638225807548 1438 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YDR455C_d YDR455C hom FF 0.0111910712130218 0.517052629382022 1439 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Unknown Unknown Unknown YNL055C POR1 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0111774863106888 0.517561510116531 1440 Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication DNA transport|mitochondrion organization|apoptotic process|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YLR262C YPT6 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|GOLGI APPARATUS|PLASMA MEMBRANE 0.0111549961082926 0.519091783478164 1441 Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 intracellular protein transport|retrograde transport, endosome to Golgi GTPase activity Golgi apparatus YMR100W MUB1 hom FT CELL DIVISION 0.0111545472983775 0.518733551774378 1442 MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene regulation of cell budding|protein monoubiquitination molecular_function cellular_component YGL137W SEC27 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0111204445962294 0.519701076314652 1443 Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP retrograde vesicle-mediated transport, Golgi to ER|late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YMR230W-A_p YMR230W-A hom 0.011102597348435 0.520371440706817 1444 Putative protein of unknown function biological_process molecular_function cellular_component YDR423C CAD1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0110908525555247 0.520812832360589 1445 AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication cellular response to cadmium ion|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YIR033W MGA2 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0110708683439922 0.521564319814695 1446 ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting positive regulation of transcription from RNA polymerase II promoter in response to hypoxia|cellular response to cold|positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|mRNA stabilization|positive regulation of transcription from RNA polymerase II promoter in response to cobalt ion|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette molecular_function integral to endoplasmic reticulum membrane|nucleus YDR318W MCM21 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0110660440684687 0.521745815673186 1447 Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function COMA complex|condensed nuclear chromosome kinetochore YHL002W HSE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0110644134855948 0.52180716777772 1448 Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT-0 complex YCL040W GLK1 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0110608387917087 0.522438852060455 1449 Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication glucose import|mannose metabolic process|glycolysis|glucose metabolic process glucokinase activity cytosol|plasma membrane YER180C-A SLO1 hom FT PROTEIN LOCALIZATION 0.0110261854064324 0.523246594623406 1450 Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO intracellular protein transport small GTPase regulator activity cytoplasm YGR035C_p YGR035C hom 0.0110010116112472 0.52419559109899 1451 Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function cellular_component YLR100W ERG27 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0109871802192902 0.524717379080036 1452 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs ergosterol biosynthetic process 3-keto sterol reductase activity mitochondrial outer membrane|endoplasmic reticulum|endoplasmic reticulum membrane YDR325W YCG1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0109785066422307 0.525044724555884 1453 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation rDNA condensation|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation molecular_function nuclear condensin complex YHR139C SPS100 hom FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS 0.0109628332166815 0.525636512964439 1454 Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin ascospore wall assembly molecular_function ascospore wall YPR039W_d YPR039W hom 0.0109306373413683 0.526853215432662 1455 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Unknown Unknown Unknown YDL217C TIM22 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0109203255844277 0.527243206858807 1456 Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YGL178W MPT5 hom FT TRANSLATION 0.0108778185398855 0.528975362446728 1457 mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity negative regulation of translation|negative regulation of translational initiation|Unknown mRNA binding|protein binding, bridging cytoplasm YOR120W GCY1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0108706758728675 0.529123021344357 1458 Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication cellular response to oxidative stress|arabinose catabolic process|D-xylose catabolic process oxidoreductase activity|mRNA binding|alditol:NADP+ 1-oxidoreductase activity|aldo-keto reductase (NADP) activity nucleus|cytoplasm YPR053C_d YPR053C hom 0.0108278329357499 0.530747855857951 1459 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Unknown Unknown Unknown YOR206W NOC2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0107854900998769 0.532662173876142 1460 Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors ribosomal subunit export from nucleus|ribosome assembly molecular_function mitochondrion|nucleus|Noc2p-Noc3p complex|Noc1p-Noc2p complex YHR134W WSS1 hom FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS 0.010778547815384 0.532620138169853 1461 Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response protein sumoylation|response to UV-B|response to UV-C|response to DNA damage stimulus molecular_function nuclear envelope YMR285C NGL2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0107778235099254 0.532708819969859 1462 Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication rRNA processing endoribonuclease activity intracellular YLL021W SPA2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.0107741850014948 0.532786037036067 1463 Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate regulation of initiation of mating projection growth|regulation of termination of mating projection growth|pseudohyphal growth|invasive filamentous growth|mating projection assembly cytoskeletal regulatory protein binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip YHR200W RPN10 hom FT PROTEOLYSIS 0.0107681949521369 0.533013855164285 1464 Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein ubiquitin-dependent protein catabolic process structural molecule activity|polyubiquitin binding proteasome complex|proteasome regulatory particle, base subcomplex YIL002W-A_p YIL002W-A hom 0.0107347169341157 0.534288022826183 1465 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YDL160C-A MHF2 hom FT RESPONSE TO DNA DAMAGE 0.0107306523147351 0.534442826108798 1466 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 response to DNA damage stimulus molecular_function cellular_component YLR330W CHS5 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.0107245050069859 0.534676992711813 1467 Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane ascospore wall assembly|conjugation with cellular fusion|cell wall chitin catabolic process|Golgi to plasma membrane transport|cellular bud site selection|regulation of transcription, DNA-dependent molecular_function mating projection tip|cytoplasm|exomer complex YCL036W GFD2 hom 0.0106898220602543 0.535999123157922 1468 Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL077C_p YPL077C hom 0.0106734199994963 0.536624950067378 1469 Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication biological_process molecular_function cellular_component YNL288W CAF40 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0106678818223942 0.536836344272963 1470 Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter molecular_function CCR4-NOT core complex YBL075C SSA3 hom FT PROTEIN LOCALIZATION 0.0106639581640018 0.536986137048019 1471 ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication protein folding|SRP-dependent cotranslational protein targeting to membrane, translocation|response to stress unfolded protein binding|ATPase activity cytosol YDR391C_p YDR391C hom FT NUCLEUS 0.0106602221892476 0.537189514441241 1472 Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YOR309C_d YOR309C hom 0.0106530101323802 0.537404209042944 1473 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Unknown Unknown Unknown YGL236C MTO1 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0106519699302449 0.537443939696907 1474 Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants mitochondrial tRNA wobble uridine modification molecular_function mitochondrion YOR352W TFB6 hom FT NUCLEUS 0.0106466741025796 0.537646237381606 1475 Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|holo TFIIH complex|cytoplasm YKL206C ADD66 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0106410800240206 0.537859969592782 1476 Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly proteasome assembly molecular_function cytosol|cytoplasm YER166W DNF1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE 0.0106335451416526 0.538147921214513 1477 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YDR071C PAA1 hom FT CHROMATIN ORGANIZATION 0.0106228417774518 0.538557091829687 1478 Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication chromatin organization diamine N-acetyltransferase activity|aralkylamine N-acetyltransferase activity cytoplasm YDR144C MKC7 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|PLASMA MEMBRANE 0.010569561829509 0.540596207277117 1479 GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p proteolysis|fungal-type cell wall organization aspartic-type endopeptidase activity fungal-type cell wall YML127W RSC9 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0105186337841572 0.542548911221615 1480 Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|rRNA transcription|regulation of transcription from RNA polymerase II promoter|chromatin remodeling|transcription elongation from RNA polymerase II promoter|response to stress chromatin binding|DNA translocase activity RSC complex YHR171W ATG7 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0105097967443938 0.542888102060271 1481 Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation C-terminal protein lipidation|protein modification by small protein conjugation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway APG8 activating enzyme activity|APG12 activating enzyme activity cytosol|mitochondrion|membrane|pre-autophagosomal structure YOR332W VMA4 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0105037723794771 0.543540856468281 1482 Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V1 domain|fungal-type vacuole membrane|integral to membrane YJR123W RPS5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION|RIBOSOME 0.0104929609542444 0.5435345998744 1483 Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 cytoplasmic translation|rRNA export from nucleus|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YIL025C_d YIL025C hom 0.010465419204562 0.54459303380005 1484 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL039W PTM1 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0104558309827449 0.544961750786563 1485 Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YER154W OXA1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0104404780955144 0.545732270507532 1486 Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals protein insertion into mitochondrial membrane from inner side|mitochondrial genome maintenance|mitochondrial proton-transporting ATP synthase complex assembly mitochondrial ribosome binding|membrane insertase activity mitochondrion|mitochondrial inner boundary membrane|integral to membrane|mitochondrial crista|mitochondrial large ribosomal subunit YOR323C PRO2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0104013110324201 0.54801892691326 1487 Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis proline biosynthetic process glutamate-5-semialdehyde dehydrogenase activity nucleus|cytoplasm YNL127W FAR11 hom FT CELL CYCLE|SIGNALING 0.0103915482864101 0.547436950194937 1488 Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YPL159C PET20 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0103317351590596 0.549745030490332 1489 Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome aerobic respiration molecular_function mitochondrion YCR006C_d YCR006C hom 0.0103097753969445 0.550593619431647 1490 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER123W YCK3 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE 0.0103083492202268 0.550946154391248 1491 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway vesicle-mediated transport|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity fungal-type vacuole membrane|plasma membrane|nucleus|fungal-type vacuole YDR301W CFT1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0103012738076549 0.55092231846374 1492 RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF mRNA cleavage|termination of RNA polymerase II transcription|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mitochondrion|mRNA cleavage and polyadenylation specificity factor complex YGR291C_d YGR291C hom 0.0102958289179998 0.551132886243242 1493 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR160C PEX18 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0102275291361065 0.553777568521877 1494 Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p protein import into peroxisome matrix protein binding cytosol|peroxisome YER020W GPA2 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0102136378150521 0.554316223015899 1495 Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery adenylate cyclase-activating G-protein coupled receptor signaling pathway|ascospore formation|replicative cell aging|signal transduction|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|regulation of cell size GTPase activity mitochondrion|plasma membrane YDR269C_d YDR269C hom 0.0102083071461751 0.554522994818033 1496 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL265C IST1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.0102012969551187 0.554794971147658 1497 Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p late endosome to vacuole transport via multivesicular body sorting pathway molecular_function endosome|cytoplasm YJR125C ENT3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|CYTOSKELETON 0.0101804533348594 0.555604031728726 1498 Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p late endosome to vacuole transport via multivesicular body sorting pathway|early endosome to Golgi transport|actin filament organization|endocytosis|actin cortical patch assembly|Golgi to endosome transport protein binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding clathrin vesicle coat|actin cortical patch YPL117C IDI1 het FT LIPID METABOLISM|NUCLEUS 0.0101801241046564 0.555616815661313 1499 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability farnesyl diphosphate biosynthetic process isopentenyl-diphosphate delta-isomerase activity nucleus|cytoplasm YMR084W_p YMR084W hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0101763461910211 0.555763521447415 1500 Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component YHR132C ECM14 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS 0.0101686281793466 0.556063289777276 1501 Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly fungal-type cell wall organization|proteolysis metalloendopeptidase activity fungal-type vacuole YDL192W ARF1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.0101343112541042 0.557573557249986 1502 ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|Golgi to plasma membrane transport|intra-Golgi vesicle-mediated transport|macroautophagy GTPase activity cytosol|Golgi-associated vesicle YIL162W SUC2 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0101292910204724 0.557592372865335 1503 Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively sucrose catabolic process beta-fructofuranosidase activity mitochondrion|extracellular region|cytoplasm YDR150W NUM1 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.010111861274756 0.558270541579899 1504 Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex mitochondrion inheritance|nuclear migration along microtubule|mitochondrial fission|microtubule cytoskeleton organization tubulin binding cell cortex|mitochondrion|cellular bud tip YPR161C SGV1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0100808935087529 0.559476444842471 1505 Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins phosphorylation of RNA polymerase II C-terminal domain|protein phosphorylation|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YPL246C RBD2 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0100758231111073 0.559674009440591 1506 Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p biological_process molecular_function COPI-coated vesicle|integral to membrane|Golgi apparatus YIL009W FAA3 hom FT KETONE METABOLISM|LIPID METABOLISM 0.0100696935541193 0.559912888614831 1507 Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery long-chain fatty acid metabolic process long-chain fatty acid-CoA ligase activity|very long-chain fatty acid-CoA ligase activity cellular_component YLR303W MET17 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0100137090531957 0.562096982812144 1508 Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis methionine metabolic process|cysteine biosynthetic process O-acetylhomoserine aminocarboxypropyltransferase activity|cysteine synthase activity plasma membrane|cytoplasm YIR044C YIR044C hom 0.0100119293316059 0.562166481585342 1509 Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family biological_process molecular_function cellular_component YJR105W ADO1 hom FF|FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0100094795161721 0.562845905179335 1510 Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle purine nucleobase metabolic process adenosine kinase activity nucleus|cytoplasm YGR195W SKI6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00995377565643211 0.564497789858053 1511 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) nuclear mRNA surveillance|U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|U1 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YGR223C HSV2 hom FT MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS 0.00994463287070802 0.56479747298623 1512 Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization piecemeal microautophagy of nucleus phosphatidylinositol binding endosome|fungal-type vacuole|extrinsic to membrane|cytoplasm YER185W PUG1 hom FT PLASMA MEMBRANE 0.00994167082719572 0.564913411559971 1513 Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins heme transport molecular_function integral to membrane|plasma membrane YNL043C_d YNL043C hom 0.00993039690270174 0.565354793520799 1514 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Unknown Unknown Unknown YDR194W-A_p YDR194W-A hom 0.00992636847448604 0.565802660621857 1515 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR068C VAM10 hom FT MEMBRANE ORGANIZATION 0.00987459266585931 0.567542003975348 1516 Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering vacuole fusion, non-autophagic molecular_function fungal-type vacuole membrane YMR222C FSH2 hom 0.00985094290664511 0.568470161055713 1517 Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm YPR063C_p YPR063C hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00982189114039065 0.56961131683017 1518 ER-localized protein of unknown function biological_process molecular_function endoplasmic reticulum YGL133W ITC1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.00976221339755104 0.571958884906836 1519 Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex negative regulation of transcription from RNA polymerase II promoter by pheromones|chromatin remodeling|chromatin silencing at telomere molecular_function chromatin accessibility complex YMR086C-A_d YMR086C-A hom 0.00975839803966161 0.572109127411895 1520 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML013W UBX2 hom FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.00974748380314607 0.572539015530706 1521 Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis proteasomal ubiquitin-dependent protein catabolic process|ER-associated protein catabolic process|lipid particle organization protein binding, bridging mitochondrial outer membrane|Doa10p ubiquitin ligase complex|integral to endoplasmic reticulum membrane|lipid particle|endoplasmic reticulum YDL025C RTK1 hom FT PROTEIN PHOSPHORYLATION 0.00974635019576874 0.572583674655482 1522 Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress biological_process protein serine/threonine kinase activity|protein kinase activity cellular_component YPR042C PUF2 hom 0.00974025006405107 0.572995840496624 1523 Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay mRNA binding cytoplasm YFR032C-A RPL29 hom FT TRANSLATION|RIBOSOME 0.00971920390523682 0.573653612117108 1524 Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|cytoplasm YHL028W WSC4 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0097132138503969 0.574175708533805 1525 ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3 response to heat|SRP-dependent cotranslational protein targeting to membrane, translocation|protein targeting to ER|response to stress transmembrane signaling receptor activity endoplasmic reticulum membrane YKL056C TMA19 hom FT TRANSLATION|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION|CYTOSKELETON 0.00967104157661621 0.575554198086039 1526 Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress cytoplasmic translation|cellular response to oxidative stress molecular_function cytosol|mitochondrion|ribosome|cytoplasm YGR175C ERG1 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00964066715982749 0.576754362845156 1527 Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine ergosterol biosynthetic process drug binding|squalene monooxygenase activity endoplasmic reticulum|lipid particle YCL057C-A MOS1 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00963982018522659 0.576787845700961 1528 Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YCR068W ATG15 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00963189411807147 0.577101225765327 1529 Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway membrane disassembly|autophagy|piecemeal microautophagy of nucleus|vacuolar protein processing|multivesicular body membrane disassembly lipase activity integral to membrane|vacuolar lumen YEL034W HYP2 het FF|FT KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION|RIBOSOME 0.00961752214697517 0.577839998477073 1530 Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication positive regulation of translational initiation|translational frameshifting|positive regulation of translational termination|positive regulation of translational elongation ribosome binding|RNA binding|translation elongation factor activity|translation initiation factor activity mitochondrion|cytosolic ribosome|cytoplasm YJL188C_d BUD19 hom 0.00961272066855708 0.577859635150098 1531 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YOR318C_d YOR318C hom 0.00960039395944953 0.578404163838477 1532 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Unknown Unknown Unknown YKL121W_p DGR2 hom 0.00959623710986382 0.578512019909217 1533 Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds biological_process molecular_function cellular_component YCR002C CDC10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00958561884483022 0.578932451781827 1534 Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress sporulation|maintenance of cell polarity|exit from mitosis|cell cycle cytokinesis|septin ring assembly protein complex scaffold|structural molecule activity|GTP binding|GTPase activity|1-phosphatidylinositol binding spindle microtubule|ascospore-type prospore|septin complex|prospore membrane|mating projection base|meiotic spindle|septin filament array|cellular bud neck septin ring|mating projection tip YMR237W BCH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.00957740424852106 0.579257808396251 1535 Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function ribosome|clathrin-coated vesicle|exomer complex YCR077C PAT1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|TRANSLATION|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|RIBOSOME 0.00957082556501276 0.579575008094134 1536 Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|chromosome segregation|regulation of translational initiation|formation of translation preinitiation complex|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly molecular_function cytosolic small ribosomal subunit|cytoplasmic mRNA processing body YKL069W YKL069W hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.00956573106763165 0.579776851330447 1537 Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress cellular response to oxidative stress methionine-R-sulfoxide reductase activity nucleus|cytoplasm YBR296C PHO89 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00955818586586095 0.580019328419332 1538 Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification phosphate ion transport|transmembrane transport sodium:inorganic phosphate symporter activity integral to membrane|plasma membrane YLL036C PRP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.00955542676837632 0.580128694951366 1539 Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain generation of catalytic spliceosome for first transesterification step ubiquitin-protein ligase activity|first spliceosomal transesterification activity mitochondrion|nucleus|Prp19 complex|cytoplasm|U2-type catalytic step 1 spliceosome YBL046W PSY4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00952593094627648 0.581298468867553 1540 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Unknown|protein dephosphorylation protein phosphatase regulator activity nucleus|cytoplasm YIL157C COA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00947855572196567 0.583179626248432 1541 Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly mitochondrial respiratory chain complex IV assembly molecular_function integral to mitochondrial membrane|mitochondrion|integral to mitochondrial inner membrane YDR075W PPH3 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0094752259302969 0.583311950992553 1542 Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Unknown|signal transduction involved in meiotic recombination checkpoint|double-strand break repair via homologous recombination|protein dephosphorylation|positive regulation of nitrogen compound metabolic process protein serine/threonine phosphatase activity condensed nuclear chromosome|nucleus|cytoplasm YDR015C_d YDR015C hom 0.00945489473848455 0.584120208692008 1543 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Unknown Unknown Unknown YPR014C_d YPR014C hom 0.00944029665095049 0.584700870527567 1544 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Unknown Unknown Unknown YER096W SHC1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0094314513446151 0.585052836950454 1545 Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication ascospore formation|chitin biosynthetic process enzyme activator activity prospore membrane YOR116C RPO31 het FF|FT NUCLEUS 0.00939597423041079 0.586465506205604 1546 RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YDR306C_p YDR306C hom FT PROTEOLYSIS 0.00937493484254954 0.587304023099769 1547 F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YML062C MFT1 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0093653431231388 0.588188371535688 1548 Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance mRNA export from nucleus|DNA recombination|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YPR153W YPR153W hom 0.00926189168952847 0.591818783348046 1549 Putative protein of unknown function biological_process molecular_function integral to membrane YBL004W UTP20 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00926164033604522 0.591828839726281 1550 Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|small-subunit processome|preribosome, small subunit precursor|nucleolus|90S preribosome|cytoplasm YKL073W LHS1 hom FT PROTEIN LOCALIZATION|NUCLEOTIDE METABOLISM|ENDOPLASMIC RETICULUM 0.0092377759954921 0.59278398293068 1551 Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway posttranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|adenyl-nucleotide exchange factor activity|ATP binding endoplasmic reticulum lumen YLR014C PPR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM|NUCLEUS 0.00922040789670681 0.593479565190176 1552 Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding|zinc ion binding nucleus YFL038C YPT1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.00921728491884226 0.593604678248432 1553 Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Golgi vesicle budding|Golgi vesicle docking|early endosome to Golgi transport|macroautophagy|endocytic recycling|protein complex assembly|CVT pathway|retrograde vesicle-mediated transport, Golgi to ER|SNARE complex disassembly|ER to Golgi vesicle-mediated transport|COPII-coated vesicle budding SNARE binding|GTPase activity trans-Golgi network|mitochondrion|Golgi stack|endoplasmic reticulum membrane|Golgi membrane|pre-autophagosomal structure YNL201C PSY2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00921492083342191 0.593754481324911 1554 Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Unknown|response to DNA damage stimulus|protein dephosphorylation|signal transduction involved in meiotic recombination checkpoint protein serine/threonine phosphatase activity condensed nuclear chromosome|nucleus YJR050W ISY1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.00916337963779618 0.595985654112346 1555 Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles generation of catalytic spliceosome for second transesterification step|mRNA 3'-splice site recognition first spliceosomal transesterification activity|second spliceosomal transesterification activity post-spliceosomal complex|Prp19 complex|post-mRNA release spliceosomal complex|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YLR279W_d YLR279W hom 0.00914624064748191 0.596454114862268 1556 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR146C ECL1 hom 0.00913213846118809 0.597020463727713 1557 Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function cellular_component YOR048C RAT1 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.00911740542243214 0.597776436328912 1558 Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination termination of RNA polymerase II transcription, exosome-dependent|nuclear mRNA surveillance|termination of RNA polymerase II transcription, poly(A)-coupled|rRNA processing|RNA processing 5'-3' exoribonuclease activity mitochondrion|nucleus YKL023W_p YKL023W hom 0.00911418913398136 0.597741668635839 1559 Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YAL041W CDC24 het FT CELL CYCLE|SIGNALING|NUCLEUS|SITE OF POLARIZED GROWTH 0.00911179154993891 0.597838033635057 1560 Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of exit from mitosis|establishment of cell polarity|small GTPase mediated signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|nucleus|cellular bud neck|mating projection tip|cellular bud tip|cytoplasm YPL193W RSA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0090691426000153 0.599553380777563 1561 Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly ribosomal large subunit assembly molecular_function nucleoplasm YPL212C PUS1 hom FT RNA PROCESSING|NUCLEUS 0.00906784413677059 0.599605640092122 1562 tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA snRNA pseudouridine synthesis|tRNA pseudouridine synthesis pseudouridine synthase activity nucleus YOR194C TOA1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00903144236556996 0.601071542199675 1563 TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription factor TFIIA complex YNL123W NMA111 hom FT LIPID METABOLISM|PROTEOLYSIS|NUCLEUS 0.00902361922511888 0.601386791725284 1564 Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases apoptotic process|cellular lipid metabolic process|protein catabolic process|cellular response to heat serine-type peptidase activity nucleus YPL101W ELP4 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.00899856845098269 0.602396765612322 1565 Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification ATPase activity|tRNA binding Elongator holoenzyme complex|cytoplasm YPL029W SUV3 hom FF|FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION|CHROMOSOME 0.00898680376570136 0.60287134670277 1566 ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron mitochondrial DNA replication|mitochondrial RNA catabolic process|Group I intron splicing exoribonuclease II activity|ATP-dependent RNA helicase activity mitochondrion|mitochondrial chromosome|mitochondrial degradosome YCR064C_d YCR064C hom FF 0.00897522614784002 0.603392591069193 1567 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Unknown Unknown Unknown YDR276C PMP3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00895465982354905 0.604871083498932 1568 Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential regulation of membrane potential|cation transport molecular_function plasma membrane YCR026C NPP1 hom FT NUCLEOTIDE METABOLISM|RESPONSE TO NUTRIENT LEVELS 0.00894404645400725 0.604597568459031 1569 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication cellular response to phosphate starvation|phosphate-containing compound metabolic process|nucleoside triphosphate metabolic process nucleotide diphosphatase activity|nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|phosphodiesterase I activity cellular_component YDR310C SUM1 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS 0.00893891925446984 0.604804715339024 1570 Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint negative regulation of transcription by competitive promoter binding|negative regulation of transcription from RNA polymerase II promoter during mitosis|DNA replication initiation|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus YPR136C_d YPR136C het 0.00892507458201262 0.605364220633628 1571 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Unknown Unknown Unknown YOL033W MSE1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00891258449692733 0.605869181720235 1572 Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated mitochondrial glutamyl-tRNA aminoacylation|glutamyl-tRNA aminoacylation|mitochondrial translation glutamate-tRNA(Gln) ligase activity|glutamate-tRNA ligase activity mitochondrion YDL136W RPL35B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00886645024294417 0.607896695583368 1573 Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YPL066W RGL1 hom FF|FT SITE OF POLARIZED GROWTH 0.00884583036375646 0.608571170332113 1574 Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source protein localization to bud neck molecular_function cellular bud neck|cytoplasm YLL008W DRS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00881516005710724 0.609814399605367 1575 Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles ribosomal large subunit assembly|rRNA processing ATP-dependent RNA helicase activity nucleolus|preribosome, large subunit precursor YBL051C PIN4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE 0.00881342596789466 0.609884725063294 1576 Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage G2/M transition of mitotic cell cycle|DNA damage checkpoint molecular_function cytoplasm YBR196C-A_p YBR196C-A hom 0.00876895603065528 0.611795684597105 1577 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR085W OST3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.008714599282557 0.613898545296271 1578 Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins protein glycosylation|protein complex assembly|protein O-linked mannosylation|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YPR055W SEC8 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00870499440081472 0.614289265030469 1579 Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Golgi inheritance|endoplasmic reticulum inheritance|Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip|exocyst|cellular bud tip YDR408C ADE8 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.00870147111294396 0.614643970264438 1580 Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process|adenine biosynthetic process phosphoribosylglycinamide formyltransferase activity nucleus|cytoplasm YKL203C TOR2 het FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.00868940582109199 0.614923630282048 1581 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication G1 phase of mitotic cell cycle|regulation of cell cycle|actin filament reorganization involved in cell cycle|positive regulation of endocytosis|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|Rho protein signal transduction|ribosome biogenesis|signal transduction|TOR signaling cascade|cytoskeleton organization|negative regulation of autophagy protein binding|protein serine/threonine kinase activity TORC1 complex|TORC2 complex|mitochondrion|fungal-type vacuole membrane|extrinsic to internal side of plasma membrane|plasma membrane|cytoplasm YJL088W ARG3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00865868104384114 0.616174794144777 1582 Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine arginine biosynthetic process|ornithine metabolic process ornithine carbamoyltransferase activity cytosol YJL136C RPS21B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00857802656907639 0.619464478644095 1583 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytoplasmic translation|endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YGR122W YGR122W hom FF|FT TRANSCRIPTION FROM RNA POL II 0.00856837379588461 0.619858702500965 1584 Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function cytoplasm YDR378C LSM6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00853037241196097 0.622036890347233 1585 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasmic mRNA processing body YKL139W CTK1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00849143186353738 0.623004946119119 1586 Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 mRNA 3'-end processing|phosphorylation of RNA polymerase II C-terminal domain|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|peptidyl-serine phosphorylation|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation protein kinase activity|cyclin-dependent protein kinase activity nucleoplasm|nucleolus|nucleus|polysome|carboxy-terminal domain protein kinase complex YMR311C GLC8 hom FT SIGNALING|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.00846115917882433 0.624244723338134 1587 Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress chromosome segregation|glycogen biosynthetic process enzyme activator activity nucleus|cytoplasm YLR020C YEH2 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.00834028173839591 0.629205677812273 1588 Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism cell wall mannoprotein biosynthetic process|sterol metabolic process sterol esterase activity integral to membrane|plasma membrane YML093W UTP14 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0083288325789711 0.62993204060084 1589 Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YMR158W MRPS8 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00832696230188023 0.629753353386624 1590 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YGR061C ADE6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.00826024673828318 0.632499656944809 1591 Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway 'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process phosphoribosylformylglycinamidine synthase activity cytoplasm YGR204W ADE3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00824991418019656 0.632925444762672 1592 Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine purine nucleobase biosynthetic process|folic acid-containing compound metabolic process methylenetetrahydrofolate dehydrogenase (NADP+) activity|formate-tetrahydrofolate ligase activity|methenyltetrahydrofolate cyclohydrolase activity nucleus|cytoplasm YCR073W-A SOL2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.00824318190012222 0.633202936454258 1593 Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication tRNA export from nucleus molecular_function|6-phosphogluconolactonase activity cytosol|cytoplasm YBL106C SRO77 hom FF|FT VESICLE-MEDIATED TRANSPORT|PLASMA MEMBRANE 0.00823899104805401 0.633375701409171 1594 Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication exocytosis|Golgi to plasma membrane transport molecular_function plasma membrane YNL319W_d YNL319W hom 0.00823303438812671 0.633621294952301 1595 Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Unknown Unknown Unknown YNL317W PFS2 het FT RNA PROCESSING|NUCLEUS 0.00822616973819425 0.634157546402739 1596 Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YNR058W BIO3 hom FT KETONE METABOLISM|COFACTOR METABOLISM 0.00821593100827233 0.634326692812738 1597 7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis biotin biosynthetic process adenosylmethionine-8-amino-7-oxononanoate transaminase activity cytoplasm YLR403W SFP1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS 0.00820186407903272 0.63490710674289 1598 Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|regulation of cell size|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription factor binding transcription factor activity nucleus|cytoplasm YOL076W MDM20 hom FF|FT MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION 0.00818130840668737 0.636007756590275 1599 Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly mitochondrion inheritance|N-terminal peptidyl-methionine acetylation|cytoskeleton organization peptide alpha-N-acetyltransferase activity NatB complex YBL037W APL3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE 0.0081804133541501 0.635792614486835 1600 Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport vesicle-mediated transport molecular_function AP-2 adaptor complex YOL115W PAP2 hom FT CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS 0.00813227486434764 0.637781714247239 1601 Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|base-excision repair|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|histone mRNA catabolic process|nuclear polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent antisense transcript catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|snoRNA polyadenylation|tRNA modification 5'-deoxyribose-5-phosphate lyase activity|polynucleotide adenylyltransferase activity nucleolus|nucleus|TRAMP complex YOL117W RRI2 hom FT SIGNALING|NUCLEUS 0.00810520874014839 0.638901243534329 1602 Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YNR030W ALG12 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00809474132594688 0.639884776424915 1603 Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation alpha-1,6-mannosyltransferase activity integral to membrane|endoplasmic reticulum YBR163W EXO5 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00806579026892845 0.640533171563385 1604 Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 mitochondrial genome maintenance single-stranded DNA specific 5'-3' exodeoxyribonuclease activity mitochondrion YBR027C_d YBR027C hom 0.0080429182343441 0.641480873498597 1605 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL108C INO4 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.00797626758447002 0.644245867971182 1606 Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain phospholipid biosynthetic process|positive regulation of transcription from RNA polymerase II promoter DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YER025W GCD11 het FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.00794022079054362 0.645743320837298 1607 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met positive regulation of translational fidelity GTP binding|tRNA binding|translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YFR034C PHO4 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00791094267382812 0.647157071561518 1608 Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability cellular response to phosphate starvation|positive regulation of phosphate metabolic process|phosphate-containing compound metabolic process|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YDL133W SRF1 hom 0.00787785014255657 0.648337707356552 1609 Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients positive regulation of catalytic activity molecular_function integral to membrane YNL170W_d YNL170W hom 0.0078580120005328 0.649163797354372 1610 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL002W DID4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.00785196022073516 0.64956232287059 1611 Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis protein retention in Golgi apparatus|intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YJL052W TDH1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00783537380252575 0.650204463440989 1612 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress gluconeogenesis|glycolysis glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YBL083C_d YBL083C hom 0.00773837970523337 0.654154582783919 1613 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Unknown Unknown Unknown YLR286C CTS1 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.00772461474879875 0.654729823375235 1614 Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p cytokinesis, completion of separation|chitin catabolic process endochitinase activity nuclear envelope|extracellular region|fungal-type cell wall|endoplasmic reticulum|cellular bud neck YPR135W CTF4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.00772085939775538 0.654886795927566 1615 Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion DNA repair|replicative cell aging|double-strand break repair via break-induced replication|DNA replication initiation|DNA-dependent DNA replication|mitotic sister chromatid cohesion chromatin binding|DNA binding nuclear chromosome|replication fork protection complex|nuclear replication fork|nucleus YPL202C AFT2 hom FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.00769399714367503 0.656058092441111 1616 Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication cellular response to oxidative stress|cellular iron ion homeostasis|positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription mitochondrion|nucleus YKL034W TUL1 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00768143346821196 0.656535707278175 1617 Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|Golgi apparatus YOR105W_p YOR105W hom 0.0076031565087642 0.659814439876833 1618 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YBL008W HIR1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.00759251595984597 0.660498264190379 1619 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores DNA replication-independent nucleosome assembly|regulation of transcription from RNA polymerase II promoter|transcription elongation from RNA polymerase II promoter nucleosome binding|transcription corepressor activity|DNA binding chromosome, centromeric region|HIR complex|nucleus YDL193W NUS1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00759077762907695 0.660761343784463 1620 Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY protein glycosylation prenyltransferase activity nuclear envelope|lipid particle|endoplasmic reticulum YGR208W SER2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.00758513383899448 0.660760205247931 1621 Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source L-serine biosynthetic process phosphoserine phosphatase activity nucleus|cytoplasm YIL046W MET30 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.00757591435686278 0.66100447485636 1622 F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus response to cadmium ion|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|regulation of DNA-dependent DNA replication initiation|M phase of mitotic cell cycle|response to arsenic-containing substance|G1/S transition of mitotic cell cycle|protein polyubiquitination|regulation of transcription involved in G1 phase of mitotic cell cycle|protein ubiquitination ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus YBL092W RPL32 het FF|FT TRANSLATION|RIBOSOME 0.00757075101861273 0.66150566776129 1623 Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YML001W YPT7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|MITOCHONDRION 0.00754844257952251 0.662346602375932 1624 Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole inheritance|Golgi to vacuole transport|vesicle-mediated transport|endocytosis GTPase activity mitochondrial outer membrane|fungal-type vacuole YMR070W MOT3 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.00745424088396763 0.666069986329721 1625 Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion cellular hyperosmotic response|negative regulation of transcription from RNA polymerase II promoter|negative regulation of ergosterol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding nucleus YHR095W_d YHR095W hom 0.00737580394904428 0.669374307064468 1626 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR044C DOG1 hom FT CARBOHYDRATE METABOLISM 0.00731659656661362 0.671872791348192 1627 2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified glucose metabolic process 2-deoxyglucose-6-phosphatase activity cellular_component YDL006W PTC1 hom FF|FT RNA PROCESSING|SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00727235346674145 0.674247172219314 1628 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation mitochondrion inheritance|inactivation of MAPK activity involved in osmosensory signaling pathway|pheromone-dependent signal transduction involved in conjugation with cellular fusion|tRNA splicing, via endonucleolytic cleavage and ligation|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YPR129W SCD6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION 0.00726765798060313 0.673940684344779 1629 Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress stress granule assembly|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly mRNA binding|RNA binding|eukaryotic initiation factor 4G binding cytoplasmic mRNA processing body|cytoplasmic stress granule|cytoplasm YOL051W GAL11 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00725870558916182 0.674548304337319 1630 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|TFIIE-class binding transcription factor activity|RNA polymerase II activating transcription factor binding|TFIIE-class transcription factor binding|TFIIH-class transcription factor binding|RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex YDR271C_d YDR271C hom 0.00721922465975068 0.675989654902244 1631 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Unknown Unknown Unknown YMR146C TIF34 het FT TRANSLATION 0.0071589445831413 0.678588432812415 1632 eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex YGR101W PCP1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00712485480884093 0.679988855578052 1633 Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases signal peptide processing|regulation of mitochondrion organization serine-type endopeptidase activity integral to membrane|mitochondrial inner membrane YNL148C ALF1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS|CYTOSKELETON 0.00692259430998715 0.688898844758267 1634 Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance protein folding|post-chaperonin tubulin folding pathway microtubule binding|alpha-tubulin binding nucleus|cytoplasm YPR115W RGC1 hom FT CELL CYCLE 0.00690709776743666 0.689251201354215 1635 Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication positive regulation of glycerol transport molecular_function cytoplasm YML071C COG8 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0068514507174805 0.691625716931854 1636 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YHL022C SPO11 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.00674770092051275 0.69606091298774 1637 Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic DNA double-strand break formation endodeoxyribonuclease activity, producing 3'-phosphomonoesters nuclear chromosome YGR119C NUP57 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00670486221878208 0.698023856593741 1638 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) NLS-bearing substrate import into nucleus|protein import into nucleus|nuclear pore organization|protein targeting to nuclear inner membrane nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YPL115C BEM3 hom FT SIGNALING|SITE OF POLARIZED GROWTH 0.00664145113917041 0.700613773351415 1639 Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly negative regulation of Rho protein signal transduction|establishment of cell polarity Rho GTPase activator activity|phosphatidylinositol-3-phosphate binding cell cortex|incipient cellular bud site|mating projection tip|cytoplasm|cellular bud tip YDL152W_d YDL152W het FF 0.00663515065882285 0.700884092218344 1640 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Unknown Unknown Unknown YLR438C-A LSM3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00657898773152718 0.703295403123931 1641 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YGR240C PFK1 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00649030203890507 0.707234191233759 1642 Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes proton transport|glycolysis 6-phosphofructokinase activity mitochondrion|6-phosphofructokinase complex|cytoplasm YLR138W NHA1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00641774418090334 0.710234788393826 1643 Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH cellular potassium ion homeostasis|cellular monovalent inorganic cation homeostasis|response to osmotic stress cation:cation antiporter activity|sodium:hydrogen antiporter activity membrane raft|integral to membrane|plasma membrane YER074W RPS24A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|MITOCHONDRION|RIBOSOME 0.00639435569554821 0.711243364551094 1644 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|mitochondrion YKL212W SAC1 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.00623859294803663 0.717973100140094 1645 Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity mitochondrial outer membrane|integral to endoplasmic reticulum membrane|mitochondrion|SPOTS complex|integral to Golgi membrane YBR023C CHS3 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|SITE OF POLARIZED GROWTH 0.00621142995827539 0.719148938640026 1646 Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan ascospore wall assembly|fungal-type cell wall chitin biosynthetic process|cytokinesis chitin synthase activity incipient cellular bud site|integral to membrane|chitosome|prospore membrane|cellular bud neck|mating projection tip|cytoplasm YDR170C SEC7 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.00619409287717799 0.719899778411765 1647 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|autophagic vacuole assembly ARF guanyl-nucleotide exchange factor activity trans-Golgi network|cytosol|late endosome|integral to membrane|Golgi-associated vesicle YDL196W_d YDL196W het FF 0.00609865630707006 0.724156287140865 1648 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Unknown Unknown Unknown YIL107C PFK26 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0060550881027778 0.725929552173143 1649 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YLR208W SEC13 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|NUCLEAR PORE 0.00604424234957646 0.726400741673959 1650 Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress positive regulation of transcription, DNA-dependent|ER-associated protein catabolic process|positive regulation of GTPase activity|nuclear pore distribution|COPII-coated vesicle budding structural molecule activity COPII vesicle coat|nuclear pore outer ring|Seh1-associated complex YCR024C SLM5 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00601099409244249 0.727845843404116 1651 Mitochondrial asparaginyl-tRNA synthetase asparaginyl-tRNA aminoacylation|mitochondrial asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity mitochondrion YLL014W EMC6 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00594144097415164 0.73106506312734 1652 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane|endoplasmic reticulum YIL143C SSL2 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|RNA LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0059261734621125 0.73153685910462 1653 Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 poly(A)+ mRNA export from nucleus|promoter clearance from RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|nucleotide-excision repair, DNA incision|transcription from RNA polymerase II promoter|phosphorylation of RNA polymerase II C-terminal domain|regulation of transposition, RNA-mediated|regulation of mitotic recombination ATP-dependent DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YJL014W CCT3 het FF 0.0059259749915849 0.731699567034665 1654 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YDR451C YHP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS|CHROMOSOME 0.00587966075403454 0.733563530781755 1655 Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II regulatory region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nuclear chromatin|nucleus YPL061W ALD6 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.00579874964356649 0.737093443173921 1656 Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress response to salt stress|NADPH regeneration|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity cytosol|mitochondrion YLR182W SWI6 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.00575098310977211 0.739329993896125 1657 Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of reciprocal meiotic recombination|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity SBF transcription complex|MBF transcription complex|nucleus|cytoplasm YGR252W GCN5 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.00573954577864872 0.739679854548989 1658 Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation histone acetylation|regulation of transcription by chromatin organization|chromatin modification|positive regulation of transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|transcription coactivator activity|histone acetyltransferase activity|H3 histone acetyltransferase activity SLIK (SAGA-like) complex|chromosome, centromeric region|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YNL051W COG5 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00573811995952495 0.74007920906517 1659 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YLR425W TUS1 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH 0.00564742177417183 0.743710281167787 1660 Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate fungal-type cell wall organization|signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|cellular bud neck YLR349W_d YLR349W hom 0.00563334174846818 0.744326904470157 1661 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Unknown Unknown Unknown YHR026W VMA16 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.00561528672895246 0.745227989532424 1662 Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane YNL089C_d YNL089C hom 0.00560679218068601 0.745490066786507 1663 Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Unknown Unknown Unknown YBL105C PKC1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00559313613576225 0.746271526892427 1664 Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) intracellular protein kinase cascade|regulation of nuclear-transcribed mRNA poly(A) tail shortening|fungal-type cell wall organization|signal transduction|actin filament organization|peroxisome degradation|protein phosphorylation|cytoplasmic mRNA processing body assembly protein kinase C activity site of polarized growth|cytoskeleton|nucleus|plasma membrane|cytoplasm YIL114C POR2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00557424319133902 0.746916861005641 1665 Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication DNA transport|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to membrane YBR255W MTC4 hom 0.00553921969164107 0.748453094907863 1666 Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm YHR209W CRG1 hom 0.00553008500379086 0.748853933682086 1667 S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin lipid homeostasis mRNA binding|S-adenosylmethionine-dependent methyltransferase activity|methyltransferase activity cellular_component YDR527W RBA50 het FF|FT TRANSCRIPTION FROM RNA POL II 0.00551224254162027 0.749637073437038 1668 Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 transcription from RNA polymerase II promoter molecular_function cytoplasm YNL332W THI12 hom 0.00537336636846229 0.755776660512816 1669 Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 thiamine biosynthetic process molecular_function cellular_component YJL207C LAA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00530545153376621 0.759011353501888 1670 AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene protein localization|retrograde transport, endosome to Golgi molecular_function AP-1 adaptor complex|ribosome|clathrin-coated vesicle YMR063W RIM9 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00525073982198058 0.761524535358654 1671 Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; ascospore formation molecular_function integral to membrane YDR187C_d YDR187C het FF 0.0051834538694198 0.764113647600284 1672 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Unknown Unknown Unknown YIL052C RPL34B hom FF|FT TRANSLATION|RIBOSOME 0.00516241866427684 0.765042700177507 1673 Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOL144W NOP8 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0051336792435126 0.766481879741653 1674 Nucleolar protein required for 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus YJL159W HSP150 hom FT CELL WALL ORG/BIOGENESIS 0.00513220951080969 0.766377525482159 1675 O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication fungal-type cell wall organization structural constituent of cell wall|ATPase activity fungal-type cell wall|extracellular region YJR118C ILM1 hom FF|FT MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00511616719732549 0.76708665523769 1676 Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth mitochondrial genome maintenance molecular_function integral to membrane|endoplasmic reticulum YCR035C RRP43 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00510874202736533 0.767414941341028 1677 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nucleoplasm|nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex)|cytoplasm YDR363W ESC2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS 0.0050763580537737 0.768847208217817 1678 Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member intra-S DNA damage checkpoint|double-strand break repair via homologous recombination|chromatin silencing at silent mating-type cassette|mitotic sister chromatid cohesion molecular_function nucleus YNL149C PGA2 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00506119606313362 0.769651750559895 1679 Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect protein transport molecular_function nuclear envelope|endoplasmic reticulum YPL118W MRP51 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00500929382678733 0.77184890578736 1680 Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial translational initiation|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YBL077W_d YBL077W het 0.00497633106433279 0.773309029886246 1681 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Unknown Unknown Unknown YMR009W ADI1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00497514888534744 0.773328516548594 1682 Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions L-methionine salvage from methylthioadenosine oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen nucleus|cytoplasm YOR224C RPB8 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00497330960470221 0.773607404541861 1683 RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YJL007C_d YJL007C hom 0.00496626600626694 0.773853586568346 1684 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR372C NDD1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.004950107469474 0.774700552510176 1685 Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes positive regulation of transcription involved in G2/M-phase of mitotic cell cycle transcription coactivator activity nucleus YNL221C POP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00493346708305448 0.775274237712531 1686 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YNL097C PHO23 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00488738466299177 0.777220643684278 1687 Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of transcription from RNA polymerase I promoter|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA|chromatin modification|positive regulation of transcription from RNA polymerase II promoter methylated histone residue binding histone deacetylase complex|Rpd3L-Expanded complex|nucleus|Rpd3L complex YOR070C GYP1 hom FF|FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.00488472883298783 0.777435560695763 1688 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion vesicle-mediated transport Rab GTPase activator activity mitochondrion|Golgi apparatus YDR248C_p YDR248C hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM 0.00483467842291086 0.77956073141851 1689 Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 biological_process molecular_function cytoplasm YDR361C BCP1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.00481831482331539 0.780383494846735 1690 Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport protein export from nucleus|ribosomal large subunit export from nucleus molecular_function nucleus|cytoplasm YGR155W CYS4 hom FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|MITOCHONDRION 0.00479808783338377 0.781473007542363 1691 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria cysteine biosynthetic process from serine|hydrogen sulfide biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|traversing start control point of mitotic cell cycle cystathionine beta-synthase activity mitochondrion|cytoplasm YKL110C KTI12 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.00478389421647553 0.781912575833652 1692 Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p regulation of transcription from RNA polymerase II promoter|cell growth|tRNA wobble uridine modification chromatin binding nucleus|cytoplasm YNL110C NOP15 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS 0.00473964591817234 0.783910946725957 1693 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm cytokinesis, actomyosin contractile ring assembly|ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YBR091C TIM12 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00469243914394576 0.785885885715369 1694 Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins protein import into mitochondrial inner membrane phospholipid binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YDR414C ERD1 hom FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00467031878688772 0.786870825006665 1695 Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) protein retention in ER lumen|protein glycosylation molecular_function integral to membrane|membrane YBL022C PIM1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.00461119764507307 0.789504944318052 1696 ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria chaperone-mediated protein complex assembly|misfolded or incompletely synthesized protein catabolic process ATP-dependent peptidase activity mitochondrion|mitochondrial matrix YBR254C TRS20 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00449012557247995 0.794906743015123 1697 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder ER to Golgi vesicle-mediated transport molecular_function TRAPP complex YNL287W SEC21 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00445035944390841 0.796683124366562 1698 Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport molecular_function endosome|COPI vesicle coat YER165W PAB1 het FF|FT RNA PROCESSING|TRANSLATION|RNA LOCALIZATION|NUCLEUS|RIBOSOME 0.00437689727380994 0.79996749610793 1699 Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G regulation of nuclear-transcribed mRNA poly(A) tail shortening|regulation of translational initiation poly(A) RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YDR297W SUR2 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00433332323636013 0.801917295065476 1700 Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis sphingolipid metabolic process sphingosine hydroxylase activity integral to membrane|endoplasmic reticulum membrane YPL195W APL5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00431217348179062 0.802864124474916 1701 Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function vesicle-mediated transport|Golgi to vacuole transport protein binding AP-3 adaptor complex YBR109C CMD1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00430496011571839 0.803187117725407 1702 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin karyogamy involved in conjugation with cellular fusion|phosphatidylinositol biosynthetic process|NLS-bearing substrate import into nucleus|microautophagy|cell budding|vacuole fusion, non-autophagic|receptor-mediated endocytosis|cytoskeleton organization|spindle pole body organization|transcription factor import into nucleus calcium-dependent protein binding|protein binding|calcium ion binding incipient cellular bud site|central plaque of spindle pole body|mating projection tip|cellular bud neck|cellular bud tip YMR043W MCM1 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|NUCLEUS|CHROMOSOME 0.0042505750172344 0.805623400002706 1703 Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes regulation of mating type switching|positive regulation of transcription involved in G2/M-phase of mitotic cell cycle|DNA replication initiation|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of mating-type specific transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA replication origin binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II repressing transcription factor binding nuclear chromatin YMR290W-A_d YMR290W-A het FF 0.00424527423945578 0.805860959577508 1704 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Unknown Unknown Unknown YOR191W ULS1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0042264703436508 0.806703818989704 1705 Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress chromatin assembly or disassembly|mating type switching SUMO binding|DNA-dependent ATPase activity mitochondrion|nucleolus|nucleus YMR077C VPS20 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.00421505983329604 0.807299993153694 1706 Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport protein binding ESCRT III complex|cytoplasm YDR389W SAC7 hom FF|FT CELL CYCLE|SIGNALING|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00421359647601861 0.807281002326767 1707 GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate actin filament reorganization involved in cell cycle|small GTPase mediated signal transduction Rho GTPase activator activity intracellular YJR059W PTK2 hom FF|FT CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|NUCLEUS|PLASMA MEMBRANE 0.00419549028069838 0.80809294780568 1708 Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake protein phosphorylation|cellular ion homeostasis|polyamine transport|G1/S transition of mitotic cell cycle|regulation of cell size protein kinase activity nucleus|plasma membrane|cytoplasm YLR268W SEC22 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00418307559052906 0.808649785651435 1709 R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion with endoplasmic reticulum|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|Golgi apparatus|endoplasmic reticulum|ER to Golgi transport vesicle membrane YJR033C RAV1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM 0.00417316373935346 0.809094432875003 1710 Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate early endosome to late endosome transport|vacuolar acidification|regulation of protein complex assembly molecular_function RAVE complex|cytoplasm|extrinsic to membrane YGR159C NSR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.00416486120250699 0.809466933431648 1711 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis ribosomal small subunit assembly|rRNA processing single-stranded DNA binding|RNA binding mitochondrion|nucleolus|nucleus YBL070C_d YBL070C hom 0.00412693717112141 0.811168975693818 1712 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR087C CSF1 hom FF|FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00409959412578813 0.812479112955026 1713 Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies fermentation molecular_function mitochondrion YNR071C_p YNR071C hom FT CARBOHYDRATE METABOLISM 0.00407938991320158 0.813304171561291 1714 Putative aldose 1-epimerase biological_process molecular_function cellular_component YBR086C IST2 hom FT PLASMA MEMBRANE 0.00406492576468828 0.81395398640922 1715 Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process response to osmotic stress lipid binding integral to membrane|cellular bud membrane|plasma membrane YDR186C YDR186C hom 0.0040585198815971 0.814241817378754 1716 Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome|cytoplasm YGR229C SMI1 hom FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|NUCLEUS|SITE OF POLARIZED GROWTH 0.00402712624170665 0.815652767163095 1717 Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity regulation of mitotic cell cycle|regulation of fungal-type cell wall biogenesis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip YGL012W ERG4 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.00400504255963777 0.816645650346566 1718 C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol ergosterol biosynthetic process delta24(24-1) sterol reductase activity integral to membrane|endoplasmic reticulum YOR171C LCB4 hom FT LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.00395290342653057 0.81899099238763 1719 Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes calcium-mediated signaling|sphingolipid metabolic process D-erythro-sphingosine kinase activity Golgi apparatus|endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum YNR043W MVD1 het FT LIPID METABOLISM 0.00392853297452578 0.820087789965536 1720 Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer sterol biosynthetic process diphosphomevalonate decarboxylase activity cytoplasm YHR195W NVJ1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0039048860217453 0.821152362644866 1721 Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) microautophagy|cellular protein localization protein binding nuclear envelope YLR215C CDC123 het FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.00389375005729685 0.821679973122985 1722 Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein regulation of mitotic cell cycle|regulation of translation molecular_function cytoplasm YOR295W UAF30 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.00383615608156136 0.824325802373254 1723 Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication transcription of nuclear large rRNA transcript from RNA polymerase I promoter|chromatin organization RNA polymerase I upstream control element sequence-specific DNA binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YPL134C ODC1 hom FT MITOCHONDRION 0.00381241698966813 0.825318411632084 1724 Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation mitochondrial transport dicarboxylic acid transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YIL087C AIM19 hom FT MITOCHONDRION 0.00376535658085211 0.827440542870187 1725 Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth biological_process molecular_function mitochondrion YCR089W FIG2 hom FT CELL WALL ORG/BIOGENESIS 0.00375327006337635 0.828036303272017 1726 Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating filamentous growth of a population of unicellular organisms|cell morphogenesis involved in conjugation with cellular fusion|invasive growth in response to glucose limitation|cytogamy molecular_function fungal-type cell wall|mating projection YPL017C IRC15 hom FT PROTEIN LOCALIZATION|CELL CYCLE|OXIDATION-REDUCTION PROCESS|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.00370498600430885 0.830164722805339 1727 Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci microtubule nucleation|mitotic recombination|microtubule anchoring|meiotic sister chromatid segregation|attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation|positive regulation of mitotic cell cycle microtubule binding|S-adenosylmethionine-dependent methyltransferase activity microtubule|cytoplasm YFR055W_p IRC7 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00363450226467495 0.833347833678521 1728 Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner sulfur compound metabolic process|L-cysteine catabolic process to pyruvate cysteine-S-conjugate beta-lyase activity cellular_component YNL052W COX5A hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00358808642954762 0.835445511300775 1729 Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDR445C_d YDR445C hom 0.00357149281177237 0.836195713916665 1730 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR230W_d YLR230W het 0.00353355354055105 0.837911518716499 1731 Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Unknown Unknown Unknown YDL119C_p YDL119C hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00344255919704751 0.842029866994596 1732 Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria transport transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YKL037W AIM26 hom FF|FT MITOCHONDRION 0.00342353581570434 0.842891403741955 1733 Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT mitochondrion degradation molecular_function mitochondrion|integral to membrane YGR095C RRP46 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0033470078732792 0.846359113783368 1734 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YCL012C YCL012C hom 0.00331738489152709 0.847904349069322 1735 Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene biological_process molecular_function cellular_component YDL011C_d YDL011C hom 0.00317638870983313 0.854294831139262 1736 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Unknown Unknown Unknown YOL067C RTG1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|NUCLEUS 0.00314446798741224 0.855550993175617 1737 Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus cellular response to oleic acid|positive regulation of transcription from RNA polymerase II promoter|mitochondria-nucleus signaling pathway sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus|cytoplasm YDL226C GCS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|MITOCHONDRION|GOLGI APPARATUS|CYTOSKELETON 0.00311415714999627 0.85692831035117 1738 ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p retrograde vesicle-mediated transport, Golgi to ER|Golgi to plasma membrane protein transport|actin filament reorganization involved in cell cycle|ER to Golgi vesicle-mediated transport actin binding|ARF GTPase activator activity|GTPase activator activity endosome|trans-Golgi network|endoplasmic reticulum-Golgi intermediate compartment|cytoskeleton YKL055C OAR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00303092860656143 0.860712423279618 1739 Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p fatty acid metabolic process|aerobic respiration 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity mitochondrion YCR052W RSC6 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.00302777537633618 0.860958626458178 1740 Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p nucleosome positioning|ATP-dependent chromatin remodeling|G2/M transition of mitotic cell cycle|G1 phase of mitotic cell cycle|nucleosome disassembly|positive regulation of transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YER052C HOM3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00299666540623704 0.862352579660248 1741 Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis threonine biosynthetic process|methionine biosynthetic process|homoserine biosynthetic process aspartate kinase activity cytoplasm YLR205C HMX1 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00291105436920772 0.866227635531395 1742 ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants response to oxidative stress|cellular iron ion homeostasis|heme catabolic process heme oxygenase (decyclizing) activity endoplasmic reticulum|membrane|nuclear outer membrane YDL035C GPR1 hom FT SIGNALING|PLASMA MEMBRANE 0.00284426876179834 0.869210703287291 1743 Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis replicative cell aging|G-protein coupled receptor signaling pathway|sucrose mediated signaling|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|invasive growth in response to glucose limitation|detection of glucose|detection of sucrose stimulus G-protein coupled receptor activity|glucose binding integral to membrane|plasma membrane YBL080C PET112 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0028410695532287 0.86935649200151 1744 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog glutaminyl-tRNAGln biosynthesis via transamidation|mitochondrial translation glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity mitochondrion|glutamyl-tRNA(Gln) amidotransferase complex YGR064W_d YGR064W hom FF 0.00281223508140758 0.870670685635526 1745 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Unknown Unknown Unknown YBR192W RIM2 het FF|FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.00279714815718838 0.871358447459372 1746 Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family pyrimidine nucleotide transport|mitochondrial genome maintenance|transmembrane transport pyrimidine nucleotide transmembrane transporter activity|transporter activity mitochondrion|integral to membrane YER171W RAD3 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.00278025990891272 0.872128440872612 1747 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress regulation of transposition, RNA-mediated|phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair, DNA incision|regulation of mitotic recombination|transcription from RNA polymerase II promoter damaged DNA binding|ATP-dependent 5'-3' DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YNL257C SIP3 hom FT TRANSCRIPTION FROM RNA POL II 0.00271496887436097 0.875106416946827 1748 Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter transcription cofactor activity integral to membrane YPR116W RRG8 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00270114043404261 0.875792547410796 1749 Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport mitochondrial genome maintenance molecular_function mitochondrion YOL001W PHO80 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00267574847396451 0.876896143755861 1750 Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling negative regulation of phosphate metabolic process|regulation of cyclin-dependent protein kinase activity|negative regulation of calcium-mediated signaling|negative regulation of transcription from RNA polymerase II promoter|cellular metal ion homeostasis|regulation of protein localization|negative regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YGR037C ACB1 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.00260917606615312 0.879935448236404 1751 Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress long-chain fatty acid transport|chronological cell aging|fatty acid metabolic process long-chain fatty acid transporter activity|fatty-acyl-CoA binding extracellular region|nucleus|cytoplasm YDR298C ATP5 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.00257114340790861 0.881672592516098 1752 Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|proton-transporting ATPase activity, rotational mechanism mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, stator stalk|mitochondrial proton-transporting ATP synthase complex YIR002C MPH1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00256733383894712 0.881934147336689 1753 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases negative regulation of strand invasion|recombinational repair|DNA replication, Okazaki fragment processing flap-structured DNA binding|3'-5' DNA helicase activity nucleus YOR236W DFR1 het FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.00250106341346718 0.884874985633143 1754 Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism tetrahydrofolate biosynthetic process|dihydrofolate metabolic process dihydrofolate reductase activity|mRNA binding cytosol YJL004C SYS1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00248457728065703 0.885696417443427 1755 Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation vesicle organization|Golgi to plasma membrane protein transport|Golgi to endosome transport molecular_function trans-Golgi network|integral to membrane|integral to Golgi membrane YMR083W ADH3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.00247017657374759 0.886286998688157 1756 Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production NADH oxidation|fermentation|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity mitochondrion|mitochondrial matrix YPL051W ARL3 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00246130607023548 0.886692586748616 1757 GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor intracellular protein transport|Golgi to plasma membrane protein transport|protein localization to organelle GTPase activity Golgi apparatus|nuclear outer membrane-endoplasmic reticulum membrane network YER111C SWI4 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS|CHROMOSOME 0.00242488353023009 0.888358252720382 1758 DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription involved in G1/S phase of mitotic cell cycle sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription SBF transcription complex|nuclear chromatin YJL095W BCK1 hom FF|FT SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|SITE OF POLARIZED GROWTH 0.00227978466104626 0.89499869720425 1759 Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p intracellular protein kinase cascade|endoplasmic reticulum unfolded protein response|peroxisome degradation|establishment of cell polarity|protein phosphorylation|response to acid|response to nutrient protein kinase activity|MAP kinase kinase kinase activity intracellular|mating projection tip YHL027W RIM101 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CELL DIVISION|ION HOMEOSTASIS|NUCLEUS 0.00226898727225795 0.895493139321263 1760 Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC meiosis|negative regulation of transcription from RNA polymerase II promoter|fungal-type cell wall biogenesis|cellular response to anoxia|response to pH|ascospore formation|barrier septum assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding nucleus YDR490C PKH1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION 0.00225825020863036 0.896046595938183 1761 Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p regulation of nuclear-transcribed mRNA poly(A) tail shortening|endocytosis|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cytosol|cell cortex|eisosome YDL115C IWR1 hom FT PROTEIN LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.00224866413103854 0.896423901757311 1762 RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress cellular protein complex localization|nucleocytoplasmic transport protein binding nucleus|DNA-directed RNA polymerase II, holoenzyme|cytoplasm YDL044C MTF2 hom FT RNA PROCESSING|TRANSLATION|MITOCHONDRION 0.00222886675490734 0.897330722155292 1763 Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription cytoplasmic translation|mRNA processing RNA binding mitochondrion YLR270W DCS1 hom FT NUCLEUS|MITOCHONDRION 0.00218706260989877 0.899246003347494 1764 Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|positive regulation of nuclease activity|nuclear-transcribed mRNA catabolic process, deadenylation-independent decay hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides|enzyme activator activity|m7G(5')pppN diphosphatase activity mitochondrion|nucleus|cytoplasmic mRNA processing body|cytoplasm YIL043C CBR1 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00217601153862006 0.899752413101661 1765 Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia biological_process cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion YDR232W HEM1 het FT COFACTOR METABOLISM|MITOCHONDRION 0.002133705621782 0.901691435613292 1766 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p heme biosynthetic process 5-aminolevulinate synthase activity mitochondrion|mitochondrial matrix YJL046W AIM22 hom FT MITOCHONDRION 0.00210242936014845 0.903125309595006 1767 Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss protein lipoylation lipoate-protein ligase activity mitochondrion YDR130C FIN1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.00199932786858375 0.90785425479044 1768 Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress intermediate filament cytoskeleton organization|mitotic cell cycle spindle checkpoint|mitotic spindle stabilization|mitotic sister chromatid segregation microtubule binding|structural constituent of cytoskeleton spindle microtubule|condensed nuclear chromosome kinetochore|intermediate filament|nucleus|spindle pole body|astral microtubule|spindle YPL042C SSN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00186302398559092 0.914149377590531 1769 Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression phosphorylation of RNA polymerase II C-terminal domain|negative regulation of transcription from RNA polymerase II promoter|negative regulation of filamentous growth|protein phosphorylation|protein destabilization|nuclear-transcribed mRNA catabolic process, non-stop decay|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II carboxy-terminal domain kinase activity|protein kinase activity|cyclin-dependent protein kinase activity mediator complex YOR207C RET1 het FF|FT NUCLEUS 0.00183506364151791 0.9153952250941 1770 Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YGR197C SNG1 hom FT PLASMA MEMBRANE 0.00181660538634612 0.916243087228008 1771 Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance nucleobase-containing compound transport molecular_function integral to membrane|plasma membrane YDR129C SAC6 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00178357684491385 0.917980817313555 1772 Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress bipolar cellular bud site selection|actin filament organization|endocytosis|response to osmotic stress actin filament binding|protein binding, bridging actin filament bundle|mating projection tip|actin cortical patch YHR155W YSP1 hom FF|FT MITOCHONDRION 0.00175481440347932 0.919082084038029 1773 Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication biological_process molecular_function mitochondrion YOR340C RPA43 het FF|FT NUCLEUS 0.00172605336865291 0.920403868447517 1774 RNA polymerase I subunit A43 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex YBR289W SNF5 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.00172574522352049 0.920418031215108 1775 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|double-strand break repair via homologous recombination|chromatin remodeling|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding SWI/SNF complex|nucleus YDR189W SLY1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00170008826338191 0.921597347927623 1776 Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|positive regulation of SNARE complex assembly SNARE binding|syntaxin binding SNARE complex|endoplasmic reticulum|ER to Golgi transport vesicle|Golgi membrane YDR477W SNF1 hom FT KETONE METABOLISM|SIGNALING|TRANSLATION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00162988041980465 0.924825309919677 1777 AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation negative regulation of translation|protein phosphorylation|replicative cell aging|signal transduction|biofilm formation|positive regulation of gluconeogenesis|pseudohyphal growth|cell adhesion|regulation of carbohydrate metabolic process|invasive growth in response to glucose limitation|cellular response to nitrogen starvation AMP-activated protein kinase activity|protein kinase activity mitochondrion|AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|fungal-type vacuole|cytoplasm YDR136C_d VPS61 hom FF 0.00162032395503265 0.925331517808668 1778 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Unknown Unknown Unknown YDL111C RRP42 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00160768838061042 0.925845898292655 1779 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) nuclear polyadenylation-dependent tRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YGR241C YAP1802 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00156600185928004 0.927763345723411 1780 Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YDR214W AHA1 hom FT NUCLEOTIDE METABOLISM 0.001565880367369 0.927801181194876 1781 Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress protein folding|response to stress ATPase activator activity|chaperone binding cytoplasm YPR170C_d YPR170C hom 0.00156263221892318 0.927918356933399 1782 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Unknown Unknown Unknown YGR209C TRX2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.00154696765617766 0.928638998671799 1783 Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress vacuole inheritance|regulation of DNA replication|cell redox homeostasis|protein deglutathionylation|sulfate assimilation|retrograde vesicle-mediated transport, Golgi to ER|cellular response to oxidative stress|ER to Golgi vesicle-mediated transport|vacuole fusion, non-autophagic disulfide oxidoreductase activity cytosol|fungal-type vacuole YBR021W FUR4 hom FF|FT PLASMA MEMBRANE 0.0014922980907749 0.931205749871683 1784 Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation uracil transport|transmembrane transport uracil:cation symporter activity integral to membrane|membrane raft|plasma membrane YJR111C_p YJR111C hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.00144774258169037 0.933205141949274 1785 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria biological_process molecular_function mitochondrion YMR080C NAM7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION|GENE SILENCING|NUCLEUS 0.00143935189323539 0.933640808382627 1786 ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|intracellular mRNA localization|translational frameshifting|regulation of translational termination|chromatin silencing at silent mating-type cassette|protein ubiquitination RNA-dependent ATPase activity|ATP-dependent RNA helicase activity|nucleic acid binding mitochondrion|polysome|cytoplasm YBL058W SHP1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.001430883617659 0.9340303326112 1787 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate glycogen metabolic process|proteasomal ubiquitin-dependent protein catabolic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|ascospore formation|sister chromatid biorientation|autophagic vacuole assembly protein phosphatase type 1 regulator activity nucleus|cytoplasm YMR154C RIM13 hom FF|FT ION HOMEOSTASIS|PROTEOLYSIS|ION HOMEOSTASIS 0.00141755023509107 0.934702158729917 1788 Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB protein processing cysteine-type endopeptidase activity cellular_component YJL213W YJL213W hom 0.00139825772027741 0.935492773716301 1789 Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p biological_process molecular_function ribosome YOR343C_d YOR343C hom 0.0013563710476008 0.937411828053559 1790 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR257W CDC31 het FF|FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON|NUCLEAR PORE 0.00134807170311246 0.937794012782717 1791 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation microtubule nucleation|proteasomal ubiquitin-dependent protein catabolic process|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton integral to membrane|transcription export complex 2|nucleotide-excision repair factor 2 complex|half bridge of spindle pole body|nuclear pore YMR216C SKY1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0013409319756437 0.938122808625431 1792 SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 cellular cation homeostasis|cellular ion homeostasis|response to drug|protein phosphorylation|positive regulation of protein import into nucleus|regulation of cell size|mRNA splice site selection protein serine/threonine kinase activity|protein kinase activity cytoplasm YGR165W MRPS35 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00130965444880361 0.939626339010876 1793 Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YHR143W-A RPC10 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00128255795517709 0.940811415978429 1794 RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YHR060W VMA22 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00120800805615887 0.94424603310622 1795 Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) vacuolar proton-transporting V-type ATPase complex assembly|vacuolar acidification unfolded protein binding extrinsic to endoplasmic reticulum membrane YMR202W ERG2 hom FF|FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00119679701627699 0.944820255302642 1796 C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis ergosterol biosynthetic process C-8 sterol isomerase activity endoplasmic reticulum YLR423C ATG17 hom FT MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.00119329681466495 0.944923924825778 1797 Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells piecemeal microautophagy of nucleus|mitochondrion degradation|peroxisome degradation|activation of protein kinase activity|autophagic vacuole assembly protein kinase activator activity|protein complex scaffold pre-autophagosomal structure|Atg1p signaling complex YMR156C TPP1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0011811800783944 0.945482292110493 1798 DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase DNA repair polynucleotide 3'-phosphatase activity cellular_component YLR321C SFH1 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.00117799700314661 0.945637079530609 1799 Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog ATP-dependent chromatin remodeling|double-strand break repair|chromosome segregation|regulation of DNA replication involved in S phase|chromatin remodeling at centromere|nucleosome disassembly|G2/M transition of mitotic cell cycle|transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YML009C-A_d YML009C-A hom 0.0011776076378798 0.945646923570108 1800 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL087C RPL23A hom FT TRANSLATION|RIBOSOME 0.00117618472628023 0.945955639461879 1801 Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YNL297C MON2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00113984681391605 0.947395062832588 1802 Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins transport|endocytosis|protein targeting to vacuole|Golgi to endosome transport guanyl-nucleotide exchange factor activity cytosol|endosome|trans-Golgi network|extrinsic to membrane YJL093C TOK1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00111939514824028 0.948329895359777 1803 Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin cellular potassium ion homeostasis potassium channel activity integral to membrane|plasma membrane YNL243W SLA2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00111137609102011 0.948699534526398 1804 Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo bipolar cellular bud site selection|fungal-type cell wall organization|actin filament organization|endocytosis|exocytosis protein binding, bridging incipient cellular bud site|mating projection tip|actin cortical patch YHR001W OSH7 hom FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM 0.00110909862046816 0.948804516752825 1805 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability maintenance of cell polarity|endocytosis|exocytosis|sterol transport|sterol metabolic process|late endosome to vacuole transport lipid binding|oxysterol binding integral to membrane|cortical endoplasmic reticulum|cytoplasm YBR111W-A SUS1 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE|HISTONE ACETYLTRANSFERASE 0.00109800487701813 0.949361234077421 1806 Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP poly(A)+ mRNA export from nucleus|regulation of protein localization|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|histone deubiquitination|histone H3-K4 methylation|transcription elongation from RNA polymerase II promoter|histone H3-K79 methylation|positive regulation of transcription from RNA polymerase II promoter enzyme activator activity DUBm complex|nucleoplasm|transcription export complex 2|SAGA complex|nuclear pore YLR406C RPL31B hom FT TRANSLATION|RIBOSOME 0.00108124583219106 0.95011080379886 1807 Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YHR162W MPC2 hom 0.00105809893594906 0.951177464643503 1808 Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|integral to mitochondrial inner membrane YGR034W RPL26B hom FT TRANSLATION|RIBOSOME 0.0010226700915032 0.952789164092427 1809 Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YER014C-A BUD25 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION 0.000998919997271954 0.95388433461225 1810 Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern cellular bud site selection molecular_function cellular_component YDR452W PPN1 hom FT NUCLEUS 0.000998000129649206 0.953926753551464 1811 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress polyphosphate catabolic process|polyphosphate metabolic process endopolyphosphatase activity|exopolyphosphatase activity fungal-type vacuole membrane|nucleus YDR328C SKP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|ION HOMEOSTASIS|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|NUCLEUS|CHROMOSOME|UBIQUITIN LIGASE COMPLEX|KINETOCHORE 0.000934004110629382 0.956878190879921 1812 Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress kinetochore assembly|protein complex assembly|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|regulation of protein complex assembly|G1/S transition of mitotic cell cycle|septin ring assembly|regulation of exit from mitosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein neddylation|vacuolar acidification|cytokinesis ubiquitin-protein ligase activity|DNA replication origin binding|protein binding RAVE complex|SCF ubiquitin ligase complex|nucleus|CBF3 complex|kinetochore|cytoplasm YIL037C PRM2 hom 0.000906922232578928 0.958127362420279 1813 Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion karyogamy involved in conjugation with cellular fusion molecular_function integral to membrane YBL089W AVT5 hom 0.00089306679294656 0.958766494700245 1814 Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication transport transporter activity integral to membrane YGR180C RNR4 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.000879457155642307 0.959424588603679 1815 Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress cofactor biosynthetic process|deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ribonucleoside-diphosphate reductase complex|nucleus|cytoplasm YPL176C TRE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.000871417010982114 0.959765222107679 1816 Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function fungal-type vacuole YGR036C CAX4 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.000865979147059523 0.960016086518641 1817 Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation lipid biosynthetic process|protein N-linked glycosylation dolichyldiphosphatase activity integral to endoplasmic reticulum membrane|integral to membrane YKL033W TTI1 het FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.000847690501510649 0.960865658548964 1818 Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ASTRA complex|mitochondrion|cytoplasm YNL229C URE2 hom FF|FT PROTEIN LOCALIZATION 0.000847595303606607 0.96086421561688 1819 Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion regulation of nitrogen utilization|response to aluminum ion|protein urmylation|cytoplasmic sequestering of transcription factor glutathione peroxidase activity|phosphoprotein binding|transcription corepressor activity cytosol YDL026W_d YDL026W hom 0.000831910847462443 0.961605027257009 1820 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCR087W_d YCR087W hom 0.000831502232454361 0.961606697495817 1821 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Unknown Unknown Unknown YNL074C MLF3 hom 0.000821900653291739 0.962049698975222 1822 Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide response to drug molecular_function cytoplasm YOR012W_p YOR012W hom 0.000821334092386335 0.96209846042729 1823 Putative protein of unknown function biological_process molecular_function cellular_component YNL213C RRG9 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.000819801249640621 0.96214656366121 1824 Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies mitochondrion organization|mitochondrial genome maintenance molecular_function mitochondrion YDR507C GIN4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.000818885772039083 0.962188803188445 1825 Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication protein autophosphorylation|budding cell bud growth|protein phosphorylation|septin checkpoint|septin ring assembly protein kinase activity cellular bud neck YBL036C YBL036C hom 0.000783767899819825 0.963809197410793 1826 Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process pyridoxal phosphate binding cellular_component YCR030C SYP1 hom FT CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.000780970032579343 0.963938301949275 1827 Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization septin cytoskeleton organization|actin cortical patch assembly molecular_function mating projection base|mating projection tip|cellular bud neck|cellular bud tip YML054C CYB2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.000770601959164857 0.964416733625097 1828 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions lactate metabolic process L-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial intermembrane space YMR041C ARA2 hom FT OXIDATION-REDUCTION PROCESS 0.000765720145576259 0.964642007911499 1829 NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase dehydro-D-arabinono-1,4-lactone biosynthetic process D-arabinose 1-dehydrogenase [NAD(P)+] activity cellular_component YEL049W PAU2 hom 0.000745906109101058 0.965556367567428 1830 Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YDR382W RPP2B hom FT TRANSLATION|RIBOSOME 0.000740376173260232 0.965811566015781 1831 Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR152C_p YOR152C hom 0.000739869719754693 0.965834938279441 1832 Putative protein of unknown function; YOR152C is not an essential gene biological_process molecular_function membrane YMR135W-A_d YMR135W-A hom 0.000729871038559007 0.966326536656061 1833 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR098C_p YJR098C hom FT LIPID METABOLISM|MITOCHONDRION 0.000710708617588065 0.967180739417512 1834 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YDL109C_p YDL109C hom FT LIPID METABOLISM 0.000689968071033872 0.968137984302767 1835 Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication cellular lipid metabolic process molecular_function cellular_component YOL141W PPM2 hom FT RNA PROCESSING|MITOCHONDRION 0.000682800933669991 0.968468782157913 1836 AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA methylation|wybutosine biosynthetic process tRNA methyltransferase activity mitochondrion|cytoplasm YJL087C TRL1 het FT RNA PROCESSING|SIGNALING|TRANSLATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.000674201192668671 0.968865708712618 1837 tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|tRNA splicing, via endonucleolytic cleavage and ligation|positive regulation of translation in response to stress GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity|RNA ligase (ATP) activity|2',3'-cyclic-nucleotide 3'-phosphodiesterase activity nuclear inner membrane|polysome YGL054C ERV14 hom FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.000669856367385123 0.969117030188767 1838 COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication ascospore formation|ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle|endoplasmic reticulum membrane YHR039C MSC7 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.000647334253072259 0.970132577139607 1839 Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids reciprocal meiotic recombination molecular_function integral to membrane|endoplasmic reticulum YER060W-A FCY22 hom 0.000642421860050457 0.970367983080905 1840 Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YAL066W_d YAL066W hom 0.000635943603998962 0.970662276151848 1841 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR262C BUD32 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|LIPID METABOLISM|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.000624546022158169 0.971157722634784 1842 Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription threonylcarbamoyladenosine metabolic process|protein phosphorylation|telomere maintenance|cellular bud site selection|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity EKC/KEOPS complex|nucleus|cytoplasm YGL146C_p RRT6 hom 0.00061004781741842 0.971826985448877 1843 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins biological_process molecular_function membrane YDR048C_d YDR048C hom 0.00059778450115568 0.972393097351241 1844 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR286W RDL2 hom FT MITOCHONDRION 0.000573396124985117 0.973518979861643 1845 Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss biological_process thiosulfate sulfurtransferase activity mitochondrion YAL061W_p BDH2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.000565550736563012 0.973881171326948 1846 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor nucleus|cytoplasm YNL067W RPL9B hom FT TRANSLATION|RIBOSOME 0.00056138855801188 0.974073325322644 1847 Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR107C TMN2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.000560687722052861 0.974105680745479 1848 Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments cellular copper ion homeostasis|pseudohyphal growth|vacuolar transport|invasive growth in response to glucose limitation molecular_function fungal-type vacuole membrane|integral to membrane YJL117W PHO86 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.000545453071388972 0.974809027756959 1849 Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress protein folding|ER to Golgi vesicle-mediated transport|regulation of phosphate transport unfolded protein binding endoplasmic reticulum YGR216C GPI1 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.000543462424008037 0.974900932594828 1850 Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YBL017C PEP1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.000540287250934515 0.975047525678348 1851 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments vacuolar transport|protein targeting to vacuole signal sequence binding integral to membrane|Golgi apparatus YPR187W RPO26 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.000539313979441241 0.975092460364541 1852 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YBL081W_p YBL081W hom 0.000501136175416016 0.97690697835179 1853 Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function cellular_component YIL042C PKP1 hom FT SIGNALING|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.00050022054059975 0.976911204346759 1854 Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p peptidyl-serine phosphorylation|protein phosphorylation|carbon utilization protein kinase activity|pyruvate dehydrogenase (acetyl-transferring) kinase activity mitochondrion YGR097W ASK10 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|RNA POL II, HOLOENZYME 0.000498517817522073 0.976976035723821 1855 Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication cellular protein catabolic process|cellular response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter|positive regulation of glycerol transport molecular_function DNA-directed RNA polymerase II, holoenzyme|cytoplasm YJR137C MET5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS 0.000487128099957355 0.977518710885098 1856 Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine fungal-type cell wall organization|sulfate assimilation sulfite reductase (NADPH) activity sulfite reductase complex (NADPH)|cytoplasm YDR094W_d YDR094W hom 0.000484031257124461 0.977644917518276 1857 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Unknown Unknown Unknown YOR345C_d YOR345C hom 0.000477279258552115 0.977956680074676 1858 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YKL122C SRP21 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEUS 0.000473271502435541 0.978141733292219 1859 Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding nucleus|signal recognition particle, endoplasmic reticulum targeting YKL220C FRE2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.000470706461299606 0.978260171534789 1860 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YGR251W NOP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.000462063103418016 0.978659273565051 1861 Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|nucleolus|nucleus|90S preribosome YGR088W CTT1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.000458459778820114 0.978825656568376 1862 Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide response to reactive oxygen species catalase activity cytoplasm YOR087W YVC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.000456120176132661 0.978933687884816 1863 Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular cation homeostasis voltage-gated ion channel activity|calcium activated cation channel activity|sodium channel activity|calcium channel activity|potassium channel activity fungal-type vacuole membrane|fungal-type vacuole YGR022C_d YGR022C hom 0.000452237563549008 0.979112968641582 1864 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Unknown Unknown Unknown YDR517W GRH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000444522568961297 0.979469213864423 1865 Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress protein secretion|ER to Golgi vesicle-mediated transport|Golgi organization molecular_function COPII vesicle coat|cis-Golgi network|cytoplasm YLR209C PNP1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.000434287746151089 0.979941820206496 1866 Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway guanosine catabolic process|inosine catabolic process|NAD biosynthesis via nicotinamide riboside salvage pathway|nicotinate nucleotide salvage nicotinamide riboside hydrolase activity|inosine nucleosidase activity|purine-nucleoside phosphorylase activity intracellular YCL023C_d YCL023C hom 0.000428571165670785 0.980205793867614 1867 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Unknown Unknown Unknown YGL223C COG1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000420506524091392 0.980598486216856 1868 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YML065W ORC1 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.000418877625852011 0.980653415907244 1869 Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding|ATPase activity|ATP binding DNA replication preinitiation complex|nucleus|pre-replicative complex|nuclear origin of replication recognition complex YGR023W MTL1 hom FT CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|PLASMA MEMBRANE 0.00041634625363282 0.980793268813285 1870 Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p cellular response to glucose starvation|fungal-type cell wall organization|cellular response to oxidative stress molecular_function integral to plasma membrane YJL058C BIT61 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.000412098412694729 0.980966465673449 1871 Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|plasma membrane|cytoplasm YBL086C_p YBL086C hom 0.00041071592430638 0.981030306432777 1872 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YOR142W LSC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.000404480102827698 0.981321053418941 1873 Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated tricarboxylic acid cycle|succinyl-CoA metabolic process succinate-CoA ligase (ADP-forming) activity mitochondrial nucleoid|mitochondrion YNR032W PPG1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.000392462134523973 0.981873243948192 1874 Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases glycogen metabolic process|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YNL218W MGS1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.000388185117626608 0.982070754277397 1875 Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress regulation of DNA repair|DNA replication, Okazaki fragment processing|DNA strand renaturation ubiquitin binding|single-stranded DNA-dependent ATPase activity|enzyme activator activity nucleus|cytoplasm YBR118W TEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|CYTOSKELETON|RIBOSOME 0.000385958274602978 0.982173589120578 1876 Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication translational elongation|tRNA export from nucleus GDP binding|GTP binding|translation elongation factor activity ribosome|eukaryotic translation elongation factor 1 complex|cytoplasm YPR131C NAT3 hom FT MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION 0.00038424510244204 0.982252703021999 1877 Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met mitochondrion inheritance|N-terminal peptidyl-methionine acetylation|cytoskeleton organization peptide alpha-N-acetyltransferase activity NatB complex|cytoplasm YDR139C RUB1 hom 0.000382804780178382 0.982319216912144 1878 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein neddylation protein tag cytoplasm YMR008C PLB1 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.000368498700176978 0.982979876375283 1879 Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol glycerophospholipid metabolic process lysophospholipase activity periplasmic space|plasma membrane YHR183W GND1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.000358391987866468 0.98345896530365 1880 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication cellular response to oxidative stress|pentose-phosphate shunt, oxidative branch phosphogluconate dehydrogenase (decarboxylating) activity mitochondrion|cytoplasm YDR023W SES1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.000352048453344881 0.983739568559203 1881 Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p seryl-tRNA aminoacylation serine-tRNA ligase activity cytoplasm YLR211C_p YLR211C hom 0.000350007607141711 0.983833818333152 1882 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron biological_process molecular_function cytoplasm YGL114W_p YGL114W hom FT PROTEIN LOCALIZATION 0.000349287182453616 0.983867088833375 1883 Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters transport oligopeptide transporter activity integral to membrane|membrane YKR023W_p YKR023W hom FT NUCLEUS|MITOCHONDRION 0.000344740373399069 0.984077069249972 1884 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YOR301W RAX1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.000336870567810476 0.984440514656718 1885 Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily cellular bud site selection molecular_function integral to membrane|cellular bud neck YMR270C RRN9 het FT NUCLEUS 0.000333660603530462 0.98459565714619 1886 Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YIL108W YIL108W hom FT PROTEOLYSIS 0.000327308149780449 0.984882133067662 1887 Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cytoplasm YLR004C THI73 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.000326072268784546 0.984948198854711 1888 Putative plasma membrane permease proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs transport transporter activity integral to membrane|endoplasmic reticulum YBR165W UBS1 hom FT PROTEIN LOCALIZATION|NUCLEUS 0.000322518539094767 0.985103332034011 1889 Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity protein export from nucleus|protein ubiquitination molecular_function nucleus YER064C VHR2 hom FT NUCLEUS 0.000320623458552663 0.985190853004478 1890 Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress regulation of transcription, DNA-dependent molecular_function nucleus YMR093W UTP15 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.000319495529963119 0.985267178796467 1891 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|90S preribosome YGL081W_p YGL081W hom 0.000319106122463805 0.985260928638487 1892 Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis biological_process molecular_function cellular_component YGR032W GSC2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE|CYTOSKELETON 0.000306221461290789 0.985855989729612 1893 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) ascospore wall assembly|(1->3)-beta-D-glucan biosynthetic process 1,3-beta-D-glucan synthase activity 1,3-beta-D-glucan synthase complex|integral to membrane|actin cap|prospore membrane YNL080C EOS1 hom FT CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.000305813776335485 0.985874818251958 1894 Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene cellular response to oxidative stress|protein N-linked glycosylation molecular_function integral to membrane|endoplasmic reticulum membrane YHR049C-A_d YHR049C-A hom 0.000295521518668558 0.986350158466623 1895 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR396C VPS33 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.000294359727068715 0.986403815249195 1896 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole regulation of SNARE complex assembly|endocytosis|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle fusion with vacuole|Golgi to vacuole transport|vacuole organization|vacuole fusion, non-autophagic|vesicle docking|protein targeting to vacuole phosphatidylinositol binding|ATP binding cytosol|fungal-type vacuole membrane|HOPS complex|fungal-type vacuole|CORVET complex YLR346C_p YLR346C hom FT MITOCHONDRION 0.000292689575042237 0.986482967342537 1897 Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene biological_process molecular_function mitochondrion YGL067W NPY1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.000288226336135991 0.986687083953173 1898 NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member NADH metabolic process NAD+ diphosphatase activity peroxisome|cytoplasm YLR328W NMA1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00028795644051212 0.986699549035274 1899 Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication NAD biosynthetic process nicotinamide-nucleotide adenylyltransferase activity nucleus|cytoplasm YDL110C TMA17 hom FT NUCLEUS 0.000286828481641433 0.986765484985778 1900 Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion biological_process molecular_function ribosome|nucleus|cytoplasm YPR011C_p YPR011C hom FT MITOCHONDRION 0.00028311998222073 0.986924871461499 1901 Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies transport transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YML077W BET5 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00028101465353172 0.987020155357284 1902 Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YLR414C PUN1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.000272950672162106 0.987392592836522 1903 Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress fungal-type cell wall organization|protein oligomerization molecular_function integral to membrane|membrane raft|plasma membrane|cellular bud|cytoplasm YGR177C ATF2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.000271619988447711 0.987465285397839 1904 Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking steroid metabolic process|response to toxin|Unknown|sterol acetylation alcohol O-acetyltransferase activity integral to endoplasmic reticulum membrane|endoplasmic reticulum|cytoplasm YML022W APT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.000270834974796674 0.987490307433244 1905 Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication AMP biosynthetic process|adenine salvage adenine phosphoribosyltransferase activity nucleus|cytoplasm YOR094W ARF3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.000269081222610988 0.987571305543523 1906 Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity intracellular protein transport|positive regulation of phosphatidylinositol biosynthetic process|endocytosis|actin filament bundle assembly|actin cortical patch localization|cellular bud site selection mRNA binding|GTPase activity cellular bud neck|cellular bud tip YGL181W GTS1 hom FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|NUCLEUS|CYTOSKELETON 0.000263072329651875 0.987848830881714 1907 Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations endocytosis|ultradian rhythm|regulation of transcription from RNA polymerase II promoter|negative regulation of phosphatidylinositol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter ARF GTPase activator activity|repressing transcription factor binding|transcription factor binding transcription factor activity nucleus|cytoplasm|actin cortical patch YJL152W_d YJL152W hom 0.000262907862367697 0.987856426950417 1908 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR041W BUD9 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.000260211644731407 0.987980954331189 1909 Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole cellular bud site selection molecular_function plasma membrane|cellular bud neck YJR153W PGU1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.00025704475335817 0.98812722060056 1910 Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins pseudohyphal growth|pectin catabolic process polygalacturonase activity extracellular region YLL063C AYT1 hom 0.000251285366535305 0.988393225067346 1911 Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis secondary metabolic process trichothecene 3-O-acetyltransferase activity cellular_component YCL074W YCL074W hom FT NUCLEUS 0.000249175779459716 0.988490659302976 1912 Pseudogene: encodes fragment of Ty Pol protein transposition, RNA-mediated ribonuclease activity|peptidase activity|RNA binding|RNA-directed DNA polymerase activity|DNA-directed DNA polymerase activity retrotransposon nucleocapsid YAR007C RFA1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.000248203808231242 0.988547529070339 1913 Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|DNA replication|double-strand break repair via homologous recombination|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex|cytoplasm YJR078W BNA2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.00024690846650923 0.988595378520447 1914 Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp de novo NAD biosynthetic process from tryptophan tryptophan 2,3-dioxygenase activity|indoleamine 2,3-dioxygenase activity cytoplasm YDR449C UTP6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.000245955110315504 0.98864110561487 1915 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YKL010C UFD4 hom FT PROTEOLYSIS|MITOCHONDRION 0.00024375678471233 0.988749345050529 1916 Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress protein monoubiquitination|protein polyubiquitination ubiquitin-protein ligase activity mitochondrion|cytoplasm YDL039C PRM7 hom 0.000236720537608668 0.989065925552227 1917 Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements conjugation with cellular fusion molecular_function integral to membrane YGR067C_p YGR067C hom FT NUCLEUS 0.000232976175007728 0.989250109484454 1918 Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p biological_process sequence-specific DNA binding cellular_component YDL218W_p YDL218W hom 0.000230662962853205 0.989345706881307 1919 Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin biological_process molecular_function integral to membrane|cytoplasm YFR014C CMK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.000229407593471903 0.989411595458943 1920 Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YFL014W HSP12 hom FT MEMBRANE ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|PLASMA MEMBRANE 0.000228378000460804 0.989451242828061 1921 Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension cellular response to oxidative stress|cell adhesion|plasma membrane organization|cellular response to osmotic stress|cellular response to heat lipid binding cytosol|endosome|nucleus|plasma membrane|cytoplasm YLR290C_p YLR290C hom FT MITOCHONDRION 0.000227338457260982 0.989499256447859 1922 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene biological_process molecular_function mitochondrion YGR179C OKP1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.000226760401997485 0.989525955235129 1923 Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 attachment of spindle microtubules to kinetochore|chromosome segregation molecular_function COMA complex|condensed nuclear chromosome kinetochore YDR330W UBX5 hom FT PROTEOLYSIS|NUCLEUS 0.000222348196914156 0.989735873514949 1924 UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p proteasomal ubiquitin-dependent protein catabolic process molecular_function nucleus|cytoplasm YPR151C SUE1 hom FT PROTEOLYSIS|MITOCHONDRION 0.00022156627598218 0.989765858192981 1925 Mitochondrial protein required for degradation of unstable forms of cytochrome c protein catabolic process molecular_function mitochondrial envelope YOR267C HRK1 hom FT ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.000220451777386693 0.989817334059879 1926 Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress protein phosphorylation|cellular ion homeostasis protein serine/threonine kinase activity|protein kinase activity cytoplasm YDR532C KRE28 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.000220222538275304 0.98982792203899 1927 Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components biological_process molecular_function chromosome, centromeric region|condensed nuclear chromosome kinetochore|spindle pole body YGR008C STF2 hom FT MITOCHONDRION 0.000218133212332161 0.989928933045095 1928 Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress cellular response to desiccation molecular_function cytoplasm YBR176W ECM31 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.000217010957738358 0.989976257069594 1929 Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate pantothenate biosynthetic process 3-methyl-2-oxobutanoate hydroxymethyltransferase activity mitochondrion YDR169C STB3 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.000211632869227531 0.9902246585645 1930 Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter by glucose sequence-specific DNA binding nucleus|cytoplasm YPL131W RPL5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00021135372503869 0.990252126067702 1931 Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YJR109C CPA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.000208059310763846 0.99038971350689 1932 Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor arginine biosynthetic process carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity carbamoyl-phosphate synthase complex YCR028C FEN2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.00020618620509599 0.990476228374265 1933 Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress pantothenate transmembrane transport|endocytosis pantothenate transmembrane transporter activity integral to membrane|plasma membrane YGL235W_p YGL235W hom 0.000203857611096193 0.990583781459306 1934 Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component YLR119W SRN2 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.00019766708351518 0.990869710334846 1935 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole protein binding endosome|ESCRT I complex YLR199C PBA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.00019366458147006 0.991054579034751 1936 Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly proteasome assembly molecular_function cytoplasm YGR246C BRF1 het FT TRANSLATION|NUCLEUS 0.000193307663280646 0.991075061466097 1937 TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB DNA-dependent transcriptional open complex formation|transposon integration|RNA polymerase III transcriptional preinitiation complex assembly|negative regulation of protein homodimerization activity TFIIIC-class transcription factor binding|RNA polymerase III core binding|DNA binding|TFIIIB-type transcription factor activity transcription factor TFIIIB complex YKL166C TPK3 hom FT SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.000192059092269747 0.991139327666285 1938 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication protein phosphorylation|mitochondrion organization|Ras protein signal transduction cAMP-dependent protein kinase activity|protein kinase activity cAMP-dependent protein kinase complex|nucleus|cytoplasm YCL022C_d YCL022C hom 0.000186886233518057 0.991367660382535 1939 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Unknown Unknown Unknown YCR013C_d YCR013C het 0.000184242485319541 0.991489771362133 1940 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Unknown Unknown Unknown YBR089W_d YBR089W het 0.000177330431979756 0.991809030236583 1941 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Unknown Unknown Unknown YLR131C ACE2 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|NUCLEUS 0.000169147834937273 0.992197472712079 1942 Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YGR010W NMA2 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.000157070842596005 0.992749132732776 1943 Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication NAD biosynthetic process nicotinamide-nucleotide adenylyltransferase activity nucleus YGR278W CWC22 het FT RNA PROCESSING|NUCLEUS 0.000151701618617654 0.992992803278986 1944 Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex|U2-type catalytic step 1 spliceosome YDR487C RIB3 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.000151589614215052 0.992997976702601 1945 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration riboflavin biosynthetic process|aerobic respiration 3,4-dihydroxy-2-butanone-4-phosphate synthase activity cytosol|mitochondrial intermembrane space YBL099W ATP1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.00014542448227296 0.993283743484758 1946 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrial nucleoid|proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, catalytic core YLR024C UBR2 hom 0.000142956599973108 0.993396731894594 1947 Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YNL166C BNI5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.000142669187876136 0.993421815364488 1948 Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner septin ring assembly|cytokinesis molecular_function cellular bud neck septin ring|cellular bud neck YCL035C GRX1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.000142040753202417 0.993439034559075 1949 Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress protein glutathionylation|cellular response to oxidative stress disulfide oxidoreductase activity|glutathione transferase activity|glutathione peroxidase activity nucleus|cytoplasm YNL157W IGO1 hom FT NUCLEUS 0.000141356369786259 0.99347064601853 1950 Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p mRNA stabilization molecular_function nucleus|cytoplasmic mRNA processing body|cytoplasm YGR292W MAL12 hom FT CARBOHYDRATE METABOLISM 0.000140497686476972 0.993515150936881 1951 Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YER071C_p TDA2 hom FT SITE OF POLARIZED GROWTH 0.000134416015658354 0.993799561267017 1952 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function mating projection tip|cytoplasm YCR102W-A_d YCR102W-A hom 0.000133502661721309 0.993833407098956 1953 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL174C TPO5 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000124041649711269 0.994270410886923 1954 Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles polyamine transport polyamine transmembrane transporter activity integral to membrane|Golgi apparatus YDR358W GGA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.000123117590368412 0.994313093247331 1955 Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Golgi to vacuole transport|protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to endosome transport ubiquitin binding trans-Golgi network YOL022C TSR4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS 0.000122105170417699 0.994359857008563 1956 Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 maturation of SSU-rRNA molecular_function cytoplasm YDL176W YDL176W hom 0.000121733192759823 0.994381234549967 1957 Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene biological_process molecular_function cellular_component YJR094W-A RPL43B hom FT TRANSLATION|RIBOSOME 0.000120787031250371 0.994420741994686 1958 Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YGR054W YGR054W hom FT TRANSLATION 0.000119656272788728 0.994472971872288 1959 Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A translational initiation translation initiation factor activity cytosolic ribosome|cytosolic small ribosomal subunit|ribosome YMR139W RIM11 hom FT PROTEOLYSIS|PROTEIN PHOSPHORYLATION 0.000118888937791067 0.994508415187222 1960 Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress proteolysis|ascospore formation|protein phosphorylation|response to stress protein serine/threonine kinase activity|protein kinase activity cytoplasm YDL197C ASF2 hom FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.000118371896128105 0.994532297422804 1961 Anti-silencing protein that causes derepression of silent loci when overexpressed chromatin silencing at silent mating-type cassette molecular_function nuclear chromosome, telomeric region|nucleus YOL075C_p YOL075C hom FT NUCLEOTIDE METABOLISM 0.000117878654056844 0.994555080356074 1962 Putative ABC transporter transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YPR026W ATH1 hom FT CARBOHYDRATE METABOLISM 0.000117800514684488 0.99455868962708 1963 Acid trehalase required for utilization of extracellular trehalose trehalose catabolic process|response to stress alpha,alpha-trehalase activity fungal-type cell wall|cell wall-bounded periplasmic space|fungal-type vacuole YKL152C GPM1 het FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.000115654291202969 0.994657824100938 1964 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis gluconeogenesis|glycolysis phosphoglycerate mutase activity cytosol|mitochondrion YLR223C IFH1 het FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|GENE SILENCING|NUCLEUS|CHROMOSOME 0.000114560088379021 0.994708365570864 1965 Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|chromatin silencing at telomere transcription coactivator activity nuclear chromatin|nucleolus|nucleus|CURI complex YML053C_p YML053C hom FT NUCLEUS 0.000112807063309088 0.994790115639453 1966 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus|cytoplasm YJL119C_d YJL119C hom 0.000110050895084034 0.994916646426049 1967 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR079C ATX2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.000108815484842938 0.994975960463505 1968 Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation cellular manganese ion homeostasis manganese ion transmembrane transporter activity integral to membrane|Golgi membrane YDL138W RGT2 hom FT SIGNALING|PLASMA MEMBRANE 0.000108613932211623 0.994983020202346 1969 Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication glucose transport|signal transduction|detection of glucose glucose transmembrane transporter activity|receptor activity|glucose binding integral to membrane|plasma membrane YDL230W PTP1 hom FT MITOCHONDRION 0.000107151369464629 0.995050576480699 1970 Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation pseudohyphal growth|invasive growth in response to glucose limitation|protein dephosphorylation protein tyrosine phosphatase activity mitochondrion|cytoplasm YNL042W BOP3 hom FT NUCLEUS 0.000105501520380898 0.995129692534964 1971 Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury cellular response to methylmercury molecular_function nucleus|cytoplasm YDR171W HSP42 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.000105185104116874 0.995148652734939 1972 Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress cytoskeleton organization unfolded protein binding cytoskeleton|chaperonin-containing T-complex|cytoplasm YML083C YML083C hom 0.000101235870068213 0.995323815797444 1973 Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component YER067W RGI1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|PLASMA MEMBRANE 0.000101055362963064 0.995332153502635 1974 Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress energy reserve metabolic process molecular_function cell periphery|nucleus|cytoplasm YDR215C_d YDR215C hom 0.000100642064538138 0.995354018877949 1975 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Unknown Unknown Unknown YGR173W RBG2 hom FT TRANSLATION 9.85290652463575e-05 0.995448844394915 1976 Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein cytoplasmic translation GTP binding cytoplasm YPL107W_p YPL107W hom FT MITOCHONDRION 9.6191621581048e-05 0.995556812115519 1977 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene biological_process molecular_function mitochondrion YPR159C-A_p YPR159C-A hom 9.35625476616668e-05 0.995678250484068 1978 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPL160W CDC60 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 9.31735944991232e-05 0.995696216452873 1979 Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA leucyl-tRNA aminoacylation|DNA replication initiation leucine-tRNA ligase activity cytoplasm YLL046C RNP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS 9.22370027449818e-05 0.99574265739688 1980 Ribonucleoprotein that contains two RNA recognition motifs (RRM) ribosome biogenesis RNA binding cytoplasm YFL021W GAT1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 9.1977364065085e-05 0.995751471033712 1981 Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p nitrogen catabolite activation of transcription from RNA polymerase II promoter|nitrogen catabolite activation of transcription sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity cytosol|nucleus YNL078W NIS1 hom FT CELL CYCLE|NUCLEUS|SITE OF POLARIZED GROWTH 9.15119594273081e-05 0.995772968350658 1982 Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network regulation of mitosis molecular_function nucleus|cellular bud neck YLR267W_p BOP2 hom 9.08779301565376e-05 0.995802254544598 1983 Protein of unknown function biological_process molecular_function cellular_component YNL047C SLM2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 8.8969578820173e-05 0.995903912726264 1984 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication eisosome assembly|TOR signaling cascade|regulation of cell growth|actin filament bundle assembly|endosomal transport|establishment or maintenance of actin cytoskeleton polarity|actin cytoskeleton organization phosphatidylinositol binding plasma membrane YKL164C PIR1 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS 8.77831835078389e-05 0.995945202836903 1985 O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication intracellular protein transport|fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall YJR091C JSN1 hom FT PROTEIN LOCALIZATION|MITOCHONDRION 8.74257132857536e-05 0.995963522386929 1986 Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|cellular protein complex localization|establishment of mitochondrion localization mRNA binding external side of mitochondrial outer membrane YDL036C PUS9 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 8.48402811212425e-05 0.996081137468076 1987 Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|nucleus|cytoplasm YER063W THO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA LOCALIZATION|NUCLEUS 8.45455952386531e-05 0.996097663338461 1988 Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation poly(A)+ mRNA export from nucleus|transcription, DNA-dependent|ribonucleoprotein complex assembly chromatin binding|double-stranded DNA binding|RNA binding nucleus YML079W_p YML079W hom FT NUCLEUS 8.30835553778883e-05 0.996185827947068 1989 Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm biological_process molecular_function nucleus|cytoplasm YGL139W FLC3 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 8.21661663459328e-05 0.996207488831635 1990 Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER FAD transport|transmembrane transport FAD transmembrane transporter activity mitochondrion|integral to membrane|endoplasmic reticulum YDR521W_d YDR521W hom 8.19919040125974e-05 0.99621440143273 1991 Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Unknown Unknown Unknown YHR180W_d YHR180W hom 8.07906812645775e-05 0.996268191381263 1992 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL189C-A COA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 7.76451740325706e-05 0.996413484751611 1993 Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p mitochondrial respiratory chain complex IV assembly molecular_function mitochondrial matrix YDR133C_d YDR133C hom 7.71183367701202e-05 0.996443138019069 1994 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Unknown Unknown Unknown YKL066W_d YKL066W hom 7.61729139724231e-05 0.996481489604176 1995 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 Unknown Unknown Unknown YLR006C SSK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 7.25703345035644e-05 0.996647895696021 1996 Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate activation of MAPKKK activity involved in osmosensory signaling pathway|positive regulation of protein autophosphorylation|osmosensory signaling pathway via two-component system protein kinase activator activity|two-component response regulator activity|protein homodimerization activity|mitogen-activated protein kinase kinase kinase binding cytoplasm YDR235W PRP42 het FT RNA PROCESSING|NUCLEUS 7.20081447128805e-05 0.99667435996574 1997 U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats mRNA splicing, via spliceosome RNA binding U1 snRNP|U2-type prespliceosome YDR037W KRS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 7.18753913304072e-05 0.996679995706504 1998 Lysyl-tRNA synthetase lysyl-tRNA aminoacylation lysine-tRNA ligase activity cytoplasm YIL053W RHR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 7.167093098041e-05 0.996689439903302 1999 Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YDR031W MIC14 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 6.93149855262649e-05 0.996798263053046 2000 Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress aerobic respiration molecular_function nucleus|mitochondrial intermembrane space|cytoplasm YLL051C FRE6 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 6.91909640216845e-05 0.996804468550511 2001 Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels intracellular sequestering of iron ion|copper ion import ferric-chelate reductase activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YJR146W_p YJR146W hom 6.84715269469033e-05 0.996837223145889 2002 Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 biological_process molecular_function cellular_component YDR097C MSH6 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 6.70035651021723e-05 0.99690502961117 2003 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p mismatch repair|meiotic mismatch repair guanine/thymine mispair binding|four-way junction DNA binding|ATPase activity|single base insertion or deletion binding|ATP binding MutSalpha complex|nucleus YDL012C YDL012C hom FT PLASMA MEMBRANE 6.55318691127076e-05 0.996973008582349 2004 Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication biological_process molecular_function plasma membrane YIL147C SLN1 het FT SIGNALING|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 5.70976283830686e-05 0.997362987706465 2005 Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators negative regulation of protein kinase activity|protein autophosphorylation|peptidyl-histidine phosphorylation|osmosensory signaling pathway via two-component system osmosensor activity|protein histidine kinase activity|histidine phosphotransfer kinase activity plasma membrane|integral to plasma membrane YPL152W-A_p YPL152W-A hom 5.69638045276074e-05 0.997368775669005 2006 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPR025C CCL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|NUCLEUS|MITOCHONDRION|RNA POL II, HOLOENZYME 5.52034771493903e-05 0.997450086955239 2007 Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|cyclin-dependent protein kinase regulator activity mitochondrion|holo TFIIH complex|TFIIK complex YLL052C AQY2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 5.38536093495973e-05 0.997512438720058 2008 Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains water transport water channel activity plasma membrane|endoplasmic reticulum membrane YDR327W_d YDR327W het 5.19066561325996e-05 0.997602370493441 2009 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Unknown Unknown Unknown YEL052W AFG1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|RESPONSE TO OXIDATIVE STRESS|PEROXISOME ORGANIZATION|MITOCHONDRION 4.94521274537215e-05 0.997715747743177 2010 Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain protein import into peroxisome matrix|cellular response to oxidative stress|misfolded or incompletely synthesized protein catabolic process molecular_function mitochondrion|mitochondrial inner membrane YOR364W_d YOR364W hom 4.89544605941088e-05 0.99773873550221 2011 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Unknown Unknown Unknown YDR459C PFA5 hom FT PLASMA MEMBRANE 4.6980323792499e-05 0.997829922989021 2012 Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain protein palmitoylation palmitoyltransferase activity integral to membrane|plasma membrane YML023C NSE5 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 4.14125199159884e-05 0.998087391343546 2013 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair molecular_function Smc5-Smc6 complex|nucleus YER089C PTC2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 4.0326312913662e-05 0.998137279058294 2014 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication endoplasmic reticulum unfolded protein response|inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of cyclin-dependent protein kinase activity|protein dephosphorylation|DNA damage checkpoint protein serine/threonine phosphatase activity nucleus|cytoplasm YGR025W_d YGR025W hom 3.74677033239898e-05 0.998270096217833 2015 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL179C COY1 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 3.65588951343573e-05 0.99831130034795 2016 Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Golgi vesicle transport molecular_function Golgi membrane YJR100C AIM25 hom FT MITOCHONDRION 3.58855043306372e-05 0.998343394499635 2017 Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YDR430C CYM1 hom FT PROTEOLYSIS|MITOCHONDRION 3.58501743349447e-05 0.99834403697274 2018 Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology proteolysis|proteolysis involved in cellular protein catabolic process metalloendopeptidase activity mitochondrion|mitochondrial intermembrane space YKR032W_d YKR032W hom 3.24868699902999e-05 0.998500959801535 2019 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR138C TPO2 hom FT PLASMA MEMBRANE 3.22985567959992e-05 0.998508980850686 2020 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication spermine transport spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|plasma membrane YOR289W_p YOR289W hom FT NUCLEUS 3.20210248595185e-05 0.998523118071042 2021 Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YDR057W YOS9 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 3.15646943411223e-05 0.998541988255378 2022 ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family ER-associated protein catabolic process oligosaccharide binding luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum lumen YGL254W FZF1 hom FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 3.07781443115365e-05 0.998578319922014 2023 Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress positive regulation of sulfite transport by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YDR016C DAD1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 3.06059989588166e-05 0.998586271517773 2024 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton condensed nuclear chromosome kinetochore|DASH complex|spindle YKL024C URA6 het FT NUCLEOTIDE METABOLISM|NUCLEUS 3.05493597808014e-05 0.99858888774787 2025 Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) 'de novo' pyrimidine nucleobase biosynthetic process|nucleobase-containing compound metabolic process uridylate kinase activity|adenylate kinase activity nucleus|cytoplasm YDL046W NPC2 hom 2.97820271620604e-05 0.998625358284064 2026 Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes intracellular sterol transport molecular_function fungal-type vacuole lumen|fungal-type vacuole YGR174W-A_p YGR174W-A hom 2.83908727052701e-05 0.998689961040253 2027 Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis biological_process molecular_function cellular_component YPR037C ERV2 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 2.83799819654731e-05 0.998689093866041 2028 Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER protein thiol-disulfide exchange thiol oxidase activity integral to endoplasmic reticulum membrane YLR016C PML1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 2.76554284997834e-05 0.998722752440408 2029 Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p mRNA export from nucleus|mRNA splicing, via spliceosome|maintenance of RNA location molecular_function RES complex|nucleus|cytoplasm YDL222C FMP45 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 2.70155899127434e-05 0.998753234260423 2030 Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C fungal-type cell wall organization|ascospore formation molecular_function cell cortex|mitochondrion|integral to membrane|plasma membrane YLR073C RFU1 hom 2.03080215703555e-05 0.999061947399432 2031 Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes regulation of ubiquitin homeostasis enzyme inhibitor activity endosome YGR077C PEX8 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 1.98802232739915e-05 0.999081707922338 2032 Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p protein targeting to peroxisome|peroxisome organization peroxisome targeting sequence binding peroxisomal matrix|peroxisomal membrane|extrinsic to intraperoxisomal membrane YMR204C INP1 hom FT PEROXISOME ORGANIZATION 1.94737077250525e-05 0.999100619575592 2033 Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane peroxisome inheritance molecular_function peroxisome|extrinsic to intraperoxisomal membrane YGL260W_p YGL260W hom 1.7620746652844e-05 0.999186197336697 2034 Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium biological_process molecular_function cellular_component YGL091C NBP35 het FT RNA PROCESSING|COFACTOR METABOLISM|NUCLEUS 1.64647108558625e-05 0.999239474608536 2035 Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases iron-sulfur cluster assembly|tRNA wobble uridine modification 4 iron, 4 sulfur cluster binding|ATPase activity|iron ion binding mitochondrion|nucleus|cytoplasm YFL027C GYP8 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM 1.60101350672159e-05 0.999260472021261 2036 GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport vesicle-mediated transport Rab GTPase activator activity intracellular YPL233W NSL1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 1.47059919293378e-05 0.99932071199372 2037 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YKR044W UIP5 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 1.3232402007381e-05 0.999388778925781 2038 Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates biological_process molecular_function nuclear envelope YER156C_p YER156C hom FT NUCLEUS 1.19771330434681e-05 0.999446761347626 2039 Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 biological_process molecular_function nucleus|cytoplasm YLR129W DIP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 1.1521619308409e-05 0.999467802089672 2040 Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YPR056W TFB4 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 1.13783524457318e-05 0.999474419763092 2041 Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YLR042C_p YLR042C hom 1.06410521306694e-05 0.999508476576274 2042 Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene biological_process molecular_function fungal-type cell wall YHL040C ARN1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 8.78875654269933e-06 0.999594036403551 2043 ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress siderophore transport siderophore transmembrane transporter activity endosome|integral to membrane|plasma membrane|cytoplasmic membrane-bounded vesicle YDL199C_p YDL199C hom 6.17730809684922e-06 0.999714790183726 2044 Putative transporter, member of the sugar porter family transport|transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YDR384C ATO3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 4.76863736278497e-06 0.999779927958241 2045 Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters ammonium transport|nitrogen utilization|transmembrane transport ammonium transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YDR461W MFA1 hom FT SIGNALING|PLASMA MEMBRANE 4.21115474334976e-06 0.999805742870072 2046 Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 pheromone-dependent signal transduction involved in conjugation with cellular fusion mating pheromone activity extracellular region YLR401C DUS3 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 1.96191741143644e-06 0.999909376590755 2047 Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress tRNA modification tRNA dihydrouridine synthase activity nucleus|cytoplasm YDR051C DET1 hom FT NUCLEUS 6.77268468281746e-07 0.9999687161257 2048 Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel intracellular sterol transport|dephosphorylation acid phosphatase activity nucleus|cytoplasm YPL012W RRP12 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME -2.02877529821201e-06 0.999906288341693 2049 Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosome biogenesis molecular_function ribosome|nucleus|90S preribosome YLR400W_d YLR400W hom -2.16903387502751e-06 0.99989980962334 2050 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER030W CHZ1 hom FT CHROMATIN ORGANIZATION|NUCLEUS -5.13718603735977e-06 0.999762706980398 2051 Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin remodeling histone binding chromatin assembly complex|nucleus YGL028C SCW11 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM -5.51783079889195e-06 0.999745124525482 2052 Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p cytokinesis, completion of separation glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall YBL065W_d YBL065W hom -6.27545840781801e-06 0.999711602842355 2053 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Unknown Unknown Unknown YGL243W TAD1 hom FT RNA PROCESSING|NUCLEUS -6.66786821272187e-06 0.999692002867905 2054 tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala tRNA modification tRNA-specific adenosine-37 deaminase activity nucleus YOR266W PNT1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION -6.97054452732977e-06 0.999678021872941 2055 Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine protein insertion into mitochondrial membrane from inner side molecular_function mitochondrion|integral to mitochondrial inner membrane YBR210W ERV15 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION -7.40403485118851e-06 0.999658049442531 2056 Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle YHL015W RPS20 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME -8.04399446800374e-06 0.99962843788754 2057 Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YLR144C ACF2 hom FT CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION -8.49514990955064e-06 0.999607598458022 2058 Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress actin cytoskeleton organization glucan endo-1,3-beta-D-glucosidase activity intracellular YMR140W SIP5 hom FT RESPONSE TO NUTRIENT LEVELS -8.53093702339924e-06 0.999605945406849 2059 Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress cellular response to glucose starvation molecular_function cytoplasm YHL012W_p YHL012W hom -9.75894717690213e-06 0.999549222095932 2060 Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL076W GPI2 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM -1.2350960432145e-05 0.999429919518203 2061 Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane|endoplasmic reticulum YLL054C_p YLL054C hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -1.42298447255777e-05 0.999342705818545 2062 Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene biological_process sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity cellular_component YOL042W NGL1 hom FT MITOCHONDRION -1.428558990878e-05 0.999340524772821 2063 Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process endonuclease activity mitochondrion YDR063W AIM7 hom FT CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON -1.48760380947961e-05 0.999312857352368 2064 Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss actin filament debranching|negative regulation of Arp2/3 complex-mediated actin nucleation molecular_function nucleus|actin cortical patch|cytoplasm YLR179C YLR179C hom FT NUCLEUS -1.60259785478257e-05 0.999259740191468 2065 Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YHR131C_p YHR131C hom -1.77351075251877e-05 0.999181527050137 2066 Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study biological_process molecular_function cytoplasm YGL134W PCL10 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY -1.85636201941239e-05 0.999142523421201 2067 Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate negative regulation of glycogen biosynthetic process|regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YDR540C IRC4 hom FF|FT NUCLEUS -1.86409175659364e-05 0.999138952961368 2068 Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus mitotic recombination molecular_function nucleus|cytoplasm YJR016C ILV3 het FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION -2.03693471108888e-05 0.999059114698277 2069 Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids branched-chain amino acid biosynthetic process dihydroxy-acid dehydratase activity mitochondrion YCR028C-A RIM1 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION -2.10030814120305e-05 0.999029841742369 2070 Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication mitochondrial DNA replication|mitochondrial genome maintenance single-stranded DNA binding mitochondrial nucleoid|mitochondrion YBL035C POL12 het FT ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME -2.20605310789703e-05 0.998980996862795 2071 B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation telomere capping|lagging strand elongation|DNA replication initiation DNA-directed DNA polymerase activity nuclear envelope|alpha DNA polymerase:primase complex|nucleus YOR096W RPS7A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME -2.23734754473166e-05 0.998966541584499 2072 Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication cytoplasmic translation|ribosome biogenesis structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YBR005W RCR1 hom FT VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -2.27370319089768e-05 0.998949748472917 2073 Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication fungal-type cell wall organization|vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane YEL066W HPA3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS -2.2961957492326e-05 0.99893935888909 2074 D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro D-amino acid metabolic process D-amino-acid N-acetyltransferase activity nucleus|cytoplasm YOR020W-A_p YOR020W-A hom FT MITOCHONDRION -2.30346056014555e-05 0.998936003185396 2075 Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YMR104C YPK2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS -2.54548996583864e-05 0.998825259846281 2076 Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication eisosome assembly|protein phosphorylation protein kinase activity nucleus|cytoplasm YML005W TRM12 hom FT RNA PROCESSING -2.77552502441603e-05 0.998717950964475 2077 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA wybutosine biosynthetic process|tRNA methylation S-adenosylmethionine-dependent methyltransferase activity|transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm YNL180C RHO5 hom FT SIGNALING|NUCLEUS|PLASMA MEMBRANE -2.86764055216599e-05 0.998675994681783 2078 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Rho protein signal transduction GTPase activity endomembrane system|fungal-type vacuole membrane|plasma membrane|nucleus|cytoplasm YLR272C YCS4 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME -2.87935959547533e-05 0.998669988546738 2079 Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation maintenance of rDNA|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation|chromatin silencing at silent mating-type cassette molecular_function nucleolus|nuclear condensin complex YMR096W SNZ1 hom FT COFACTOR METABOLISM -2.92170984614846e-05 0.998651433534342 2080 Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins pyridoxine metabolic process|thiamine biosynthetic process protein binding cellular_component YPL019C VTC3 hom FT MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOPLASMIC RETICULUM -2.95726836634482e-05 0.998634001566206 2081 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion polyphosphate metabolic process|vacuolar transport|microautophagy|vacuole fusion, non-autophagic molecular_function fungal-type vacuole membrane|integral to membrane|vacuolar transporter chaperone complex|endoplasmic reticulum YKL127W PGM1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY -3.46504253696199e-05 0.998399454756771 2082 Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism glucose 6-phosphate metabolic process|galactose catabolic process|glucose 1-phosphate metabolic process|UDP-glucose metabolic process|trehalose biosynthetic process|glycogen biosynthetic process phosphoglucomutase activity cytoplasm YJR119C JHD2 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS -3.75938328833193e-05 0.998263495394055 2083 JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation histone demethylation histone demethylase activity (H3-K4 specific) nucleus|cytoplasm YLR226W BUR2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS -3.86091698981725e-05 0.998216595826457 2084 Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain|positive regulation of histone ubiquitination|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation|mitotic sister chromatid segregation cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YEL004W YEA4 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -3.88268320052186e-05 0.998207344627734 2085 Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER fungal-type cell wall chitin biosynthetic process|UDP-N-acetylglucosamine transport|UDP-glucose transport|transmembrane transport UDP-N-acetylglucosamine transmembrane transporter activity integral to membrane|endoplasmic reticulum YDL094C_d YDL094C hom -4.01673236361139e-05 0.998144622951172 2086 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Unknown Unknown Unknown YLR178C TFS1 hom FT SIGNALING|PROTEOLYSIS -4.13233837442442e-05 0.998091223243034 2087 Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress regulation of Ras protein signal transduction|regulation of proteolysis lipid binding|peptidase inhibitor activity|phospholipid binding fungal-type vacuole lumen|fungal-type vacuole membrane|cytoplasm YKL074C MUD2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -4.3033041222951e-05 0.998012252269511 2088 Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 mRNA branch site recognition pre-mRNA branch point binding commitment complex YNL039W BDP1 het FT NUCLEUS -4.45465382243121e-05 0.997942649179902 2089 Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs DNA-dependent transcriptional open complex formation|positive regulation of transposon integration|RNA polymerase III transcriptional preinitiation complex assembly TFIIIC-class transcription factor binding|TFIIIB-type transcription factor activity transcription factor TFIIIB complex YKL202W_d YKL202W hom -4.49351419400004e-05 0.997924392152458 2090 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR252W BTT1 hom FT PROTEIN LOCALIZATION|NUCLEUS -4.56830910260681e-05 0.997889843571162 2091 Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 'de novo' cotranslational protein folding unfolded protein binding nascent polypeptide-associated complex|CCR4-NOT complex YGR011W_d YGR011W hom -4.62162004243591e-05 0.997865218670982 2092 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL082W RPL13A hom FT TRANSLATION|RIBOSOME -4.80822846550745e-05 0.997779022236556 2093 Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL091C UBX3 hom -4.8186049743516e-05 0.997774229216449 2094 Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10 biological_process molecular_function cytoplasm YJR106W ECM27 hom FT CELL WALL ORG/BIOGENESIS -4.86438540231215e-05 0.997753082749457 2095 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p fungal-type cell wall organization molecular_function integral to membrane YNL158W PGA1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM -4.97161853716645e-05 0.997703550629207 2096 Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes GPI anchor biosynthetic process mannosyltransferase activity integral to endoplasmic reticulum membrane|nuclear envelope|mannosyltransferase complex YDR219C MFB1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX -4.9991470070136e-05 0.997690834938965 2097 Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|mitochondrion organization molecular_function extrinsic to mitochondrial outer membrane|SCF ubiquitin ligase complex YJR022W LSM8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -5.29714284389043e-05 0.997553187560638 2098 Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|nucleus|U4/U6 x U5 tri-snRNP complex YOR155C ISN1 hom FT NUCLEOTIDE METABOLISM -5.37160942600963e-05 0.997518790682584 2099 Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms nicotinamide riboside biosynthetic process|inosine salvage|nicotinic acid riboside biosynthetic process IMP 5'-nucleotidase activity cellular_component YNR073C_p YNR073C hom -5.49647209660125e-05 0.997462251900517 2100 Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p biological_process mannitol dehydrogenase activity cellular_component YKR071C DRE2 het FT COFACTOR METABOLISM|MITOCHONDRION -5.50372159310389e-05 0.997457766728964 2101 Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress iron-sulfur cluster assembly electron carrier activity cytosol|cytoplasm|mitochondrial intermembrane space YML113W DAT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -5.55082559892598e-05 0.997436008913183 2102 DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability negative regulation of transcription from RNA polymerase II promoter AT DNA binding nucleus YML070W DAK1 hom FT CARBOHYDRATE METABOLISM -5.55298305662401e-05 0.997435012361739 2103 Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation response to stress|glycerol catabolic process glycerone kinase activity cytoplasm YAL034C-B_d YAL034C-B het -5.91360681592135e-05 0.997268436672024 2104 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR254W ENO1 hom FT MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -6.06170292776558e-05 0.997201283076108 2105 Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|gluconeogenesis|glycolysis phosphopyruvate hydratase activity mitochondrion|phosphopyruvate hydratase complex|plasma membrane|fungal-type vacuole|cytoplasm YLR117C CLF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|NUCLEUS|CHROMOSOME -6.0939449905329e-05 0.997185136725443 2106 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins cis assembly of pre-catalytic spliceosome|DNA replication initiation DNA replication origin binding|chromatin binding|first spliceosomal transesterification activity|second spliceosomal transesterification activity chromatin|U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YDL174C DLD1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -6.09430304660326e-05 0.997184971335913 2107 D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane cellular carbohydrate metabolic process|aerobic respiration D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial inner membrane YOR261C RPN8 het FT PROTEOLYSIS -6.09508468483333e-05 0.997184610289723 2108 Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p ubiquitin-dependent protein catabolic process molecular_function proteasome storage granule|proteasome regulatory particle, lid subcomplex YDR365C ESF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -6.16475032572024e-05 0.997154981116198 2109 Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels rRNA processing RNA binding nucleolus|nucleus YOR082C_d YOR082C hom -6.41337967800291e-05 0.997037586830464 2110 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Unknown Unknown Unknown YGR207C CIR1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -6.62657970231282e-05 0.996939107784417 2111 Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response biological_process molecular_function mitochondrion YIR009W MSL1 hom FT RNA PROCESSING|NUCLEUS -6.68240746359944e-05 0.996913320437237 2112 U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members mRNA splicing, via spliceosome U2 snRNA binding U2 snRNP|U2-type prespliceosome YLR216C CPR6 hom -6.78662879583205e-05 0.996865179671185 2113 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding|protein refolding unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytoplasm YDR374C_p YDR374C hom -6.82452317935563e-05 0.996851440431761 2114 Putative protein of unknown function biological_process molecular_function cellular_component YML116W-A_d YML116W-A hom -6.87586409984138e-05 0.996824434951075 2115 Putative protein of unknown function Unknown Unknown Unknown YKR039W GAP1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|PLASMA MEMBRANE -7.09106701732294e-05 0.996724556998182 2116 General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth amino acid transport|gamma-aminobutyric acid transport|ammonia assimilation cycle|polyamine transport amino acid transmembrane transporter activity|polyamine transmembrane transporter activity|L-proline transmembrane transporter activity endosome|fungal-type vacuole lumen|integral to membrane|plasma membrane|ER to Golgi transport vesicle|integral to plasma membrane|multivesicular body YMR309C NIP1 het FT TRANSLATION -7.24735474693918e-05 0.996653864966501 2117 eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YER011W TIR1 hom -7.32030086623095e-05 0.996618671948475 2118 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking response to stress structural constituent of cell wall fungal-type cell wall YLR410W VIP1 hom FT CYTOSKELETON -7.40929556556983e-05 0.996577564561609 2119 Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 inositol phosphate biosynthetic process inositol hexakisphosphate 1-kinase activity|inositol hexakisphosphate 3-kinase activity|inositol heptakisphosphate kinase activity|inositol hexakisphosphate 4-kinase activity|inositol hexakisphosphate 6-kinase activity cytoplasm YOL072W THP1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE -7.41857381943464e-05 0.996573278860016 2120 Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding mRNA export from nucleus|mRNA 3'-end processing|transcription-coupled nucleotide-excision repair|cellular bud site selection|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|transcription elongation from RNA polymerase II promoter double-stranded DNA binding|RNA binding nuclear envelope|nucleus|transcription export complex 2|nuclear pore YKL173W SNU114 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEOTIDE METABOLISM|NUCLEUS -7.51960958741255e-05 0.996526609616162 2121 GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 assembly of spliceosomal tri-snRNP|spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step GTP binding|GTPase activity|U5 snRNA binding U4/U6 x U5 tri-snRNP complex|U5 snRNP YHL042W_p YHL042W hom -7.52411674190316e-05 0.996524527725082 2122 Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component YLR076C_d YLR076C het -7.57892951045659e-05 0.996509146478717 2123 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Unknown Unknown Unknown YJR147W HMS2 hom FT NUCLEUS -7.58685753345165e-05 0.996495547248934 2124 Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication pseudohyphal growth molecular_function nucleus|cytoplasm YAL059W ECM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|CELL WALL ORG/BIOGENESIS|NUCLEUS -7.82290736542053e-05 0.996386513975062 2125 Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm fungal-type cell wall organization|ribosomal large subunit export from nucleus molecular_function nucleolus|nucleus|preribosome, large subunit precursor YNL014W HEF3 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME -7.88567772711598e-05 0.996357519862587 2126 Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication translational elongation ATPase activity|translation elongation factor activity cytosolic ribosome YHR035W_p YHR035W hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM -7.95073092781395e-05 0.996327471294306 2127 Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component YER018C SPC25 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE -8.07126022185671e-05 0.996271797910279 2128 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YIR014W_p YIR014W hom -8.0905186553724e-05 0.996262902297212 2129 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene biological_process molecular_function fungal-type vacuole YLR250W SSP120 hom -8.09259160815246e-05 0.996261944784998 2130 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm YGR194C XKS1 hom FT CARBOHYDRATE METABOLISM -8.10501458342676e-05 0.996256206520994 2131 Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains xylulose catabolic process xylulokinase activity cytoplasm YHR003C YHR003C hom FT MITOCHONDRION -8.21451398816393e-05 0.996205627945862 2132 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion YDR400W URH1 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS -8.56593271784991e-05 0.996043305170847 2133 Uridine nucleosidase (uridine-cytidine N-ribohydrolase), cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways pyrimidine-containing compound salvage|NAD biosynthesis via nicotinamide riboside salvage pathway|pyrimidine nucleoside catabolic process|nicotinate nucleotide salvage ribosylpyrimidine nucleosidase activity|nicotinic acid riboside hydrolase activity|nicotinamide riboside hydrolase activity|uridine nucleosidase activity nucleus|cytoplasm YOR010C TIR2 hom -8.57564747077415e-05 0.996038817860656 2134 Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis response to stress molecular_function fungal-type cell wall YGL150C INO80 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME -8.65309356773362e-05 0.996003044988281 2135 ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription nucleosome positioning|histone exchange|transcription from RNA polymerase II promoter|DNA repair|nucleosome mobilization|chromatin remodeling|chromatin silencing at telomere|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter ATPase activity|ATP-dependent 3'-5' DNA helicase activity Ino80 complex YMR297W PRC1 hom FT PROTEOLYSIS|ENDOPLASMIC RETICULUM -8.69620252254651e-05 0.995983132675974 2136 Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family vacuolar protein catabolic process|phytochelatin biosynthetic process serine-type carboxypeptidase activity fungal-type vacuole lumen|endoplasmic reticulum|fungal-type vacuole|cytoplasm YOR304W ISW2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS -8.77721674857911e-05 0.995945711674119 2137 ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth negative regulation of transcription from RNA polymerase II promoter by pheromones|nucleosome positioning|termination of RNA polymerase II transcription|negative regulation of antisense RNA transcription|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere single-stranded DNA binding|DNA translocase activity chromatin accessibility complex|nucleus YDL017W CDC7 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|PROTEIN PHOSPHORYLATION|GENE SILENCING|NUCLEUS -8.81597766605604e-05 0.995927807755501 2138 DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p negative regulation of exit from mitosis|regulation of chromatin silencing at telomere|protein phosphorylation|double-strand break repair via break-induced replication|DNA replication initiation protein serine/threonine kinase activity nucleus|Dbf4-dependent protein kinase complex YOL150C_d YOL150C hom -9.23062078581558e-05 0.995736281545239 2139 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL086W_p YDL086W hom FT MITOCHONDRION -9.2659844184434e-05 0.995722501726737 2140 Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene biological_process carboxymethylenebutenolidase activity mitochondrion|cytoplasm YNL092W_p YNL092W hom -9.49976647912891e-05 0.995611961622924 2141 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YLL012W YEH1 hom FT LIPID METABOLISM -9.55756128586464e-05 0.99558526588767 2142 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes sterol metabolic process sterol esterase activity integral to membrane|lipid particle YEL060C PRB1 hom FT PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS -9.66861700592155e-05 0.995533968661057 2143 Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress vacuolar protein catabolic process|sporulation resulting in formation of a cellular spore|cellular response to starvation serine-type endopeptidase activity fungal-type vacuole lumen|fungal-type vacuole YDL008W APC11 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -9.719436493195e-05 0.995510494876261 2144 Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YAL043C PTA1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS -9.85784179451756e-05 0.995446564769359 2145 Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII tRNA processing|termination of RNA polymerase II transcription, exosome-dependent|mRNA cleavage|termination of RNA polymerase II transcription, poly(A)-coupled|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YKL140W TGL1 hom FT LIPID METABOLISM -9.88971935741059e-05 0.99543184036637 2146 Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes cellular lipid metabolic process|sterol metabolic process lipase activity|sterol esterase activity integral to membrane|lipid particle YOL045W PSK2 hom FT SIGNALING|TRANSLATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION -9.93268728966569e-05 0.995413362400184 2147 PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication regulation of (1->6)-beta-D-glucan biosynthetic process|negative regulation of glycogen biosynthetic process|protein phosphorylation protein serine/threonine kinase activity mitochondrion|cytoplasm YAL054C ACS1 hom FT KETONE METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION -9.9867598851479e-05 0.995387705119669 2148 Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions acetate fermentation|histone acetylation|acetyl-CoA biosynthetic process acetate-CoA ligase activity|acid-ammonia (or amide) ligase activity cytosol|mitochondrion|integral to membrane YNL020C ARK1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000100618919419709 0.99535300644831 2149 Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|cytokinesis|actin cortical patch assembly protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YEL054C RPL12A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME -0.00010272482117214 0.995255040489078 2150 Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YMR247W-A_p YMR247W-A hom -0.000103364055672642 0.99522765130189 2151 Putative protein of unknown function biological_process molecular_function cellular_component YNL312W RFA2 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME -0.000104910801429555 0.995154069160597 2152 Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YNR021W_p YNR021W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.00010524405109353 0.995139401519222 2153 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene biological_process molecular_function endoplasmic reticulum YHL043W ECM34 hom FT CELL WALL ORG/BIOGENESIS -0.0001053080217821 0.995135721397182 2154 Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins fungal-type cell wall organization molecular_function cellular_component YLR118C YLR118C hom FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS -0.000107791008617834 0.995021031322932 2155 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS protein deacylation palmitoyl-(protein) hydrolase activity nucleus|cytoplasm YDR044W HEM13 het FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM -0.00010796647896101 0.995015158776443 2156 Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) heme biosynthetic process coproporphyrinogen oxidase activity cytosol YGR211W ZPR1 het FT CELL CYCLE|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000111115487004282 0.994871302512388 2157 Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress regulation of mitotic cell cycle|cellular response to starvation protein binding nucleus|cytoplasm YDL123W SNA4 hom -0.000111841977296894 0.994833915813038 2158 Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated biological_process molecular_function fungal-type vacuole lumen|fungal-type vacuole membrane YPL027W SMA1 hom FT MEMBRANE ORGANIZATION -0.000112908915383669 0.994784633656042 2159 Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p ascospore-type prospore membrane assembly molecular_function prospore membrane YML051W GAL80 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEUS -0.000112967566962053 0.994782703040077 2160 Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding negative regulation of kinase activity|galactose metabolic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity RNA polymerase II activating transcription factor binding|kinase inhibitor activity nucleus|cytoplasm YNL023C FAP1 hom FT NUCLEUS -0.000114711839666693 0.994701356144123 2161 Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 biological_process sequence-specific DNA binding transcription factor activity cytoplasm YDL131W LYS21 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS|MITOCHONDRION -0.000115175351322928 0.994679946437073 2162 Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication lysine biosynthetic process via aminoadipic acid homocitrate synthase activity nucleus YDL054C MCH1 hom -0.000115179733081144 0.994680537802795 2163 Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport transport monocarboxylic acid transmembrane transporter activity|transporter activity integral to membrane|membrane YOR054C VHS3 hom FT CELL CYCLE|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|COFACTOR METABOLISM -0.000116094190359944 0.99463750506782 2164 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis coenzyme A biosynthetic process|cellular monovalent inorganic cation homeostasis phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex YOL080C REX4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS -0.000120554119806614 0.99443150020204 2165 Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly ribosomal large subunit assembly|rRNA processing 3'-5' exonuclease activity nucleolus|nucleus YJR072C NPA3 het FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION -0.000120609998892876 0.994428919140292 2166 Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress cellular protein complex localization|nucleocytoplasmic transport|mitotic sister chromatid cohesion ATPase activity|GTPase activity cytoplasm YLR298C YHC1 het FT RNA PROCESSING|NUCLEUS -0.000121657502048951 0.994380534853714 2167 Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YDR530C APA2 hom FT NUCLEOTIDE METABOLISM|NUCLEUS -0.000121856395959285 0.994371347924337 2168 Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication nucleoside catabolic process bis(5'-nucleosyl)-tetraphosphatase activity nucleus|cytoplasm YLR459W GAB1 het FT CELL CYCLE|LIPID METABOLISM|CELL DIVISION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000123538395103435 0.994293656247563 2169 GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|GPI-anchor transamidase complex YOL125W TRM13 hom FT RNA PROCESSING|NUCLEUS -0.000127777067205203 0.99409787188121 2170 2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases tRNA methylation tRNA methyltransferase activity nucleus|cytoplasm YIL089W YIL089W hom FT ENDOPLASMIC RETICULUM -0.000129871634974078 0.994002018879788 2171 Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking biological_process molecular_function fungal-type vacuole lumen|integral to membrane|endoplasmic reticulum YDR368W YPR1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS -0.00013195526622522 0.993904881115562 2172 NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication cellular response to oxidative stress|D-xylose catabolic process|arabinose catabolic process glucose 1-dehydrogenase (NADP+) activity|alditol:NADP+ 1-oxidoreductase activity|carbonyl reductase (NADPH) activity nucleus|cytoplasm YOR239W ABP140 hom FT RNA PROCESSING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000133759581894905 0.993821539991022 2173 AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift actin filament bundle assembly|tRNA methylation tRNA (cytosine-3-)-methyltransferase activity|actin filament binding|protein binding, bridging actin filament|actin filament bundle|mating projection tip|actin cortical patch YGR003W CUL3 hom FT PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX -0.000137209227015873 0.993663147051995 2174 Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity nucleus|Cul3-RING ubiquitin ligase complex|cytoplasm YDR153C ENT5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM -0.000137642452796232 0.993642190879152 2175 Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin late endosome to vacuole transport via multivesicular body sorting pathway|early endosome to Golgi transport|Golgi to endosome transport clathrin binding|phosphatidylinositol-3,5-bisphosphate binding endosome|clathrin vesicle coat|cytoplasm YEL016C NPP2 hom FT NUCLEOTIDE METABOLISM -0.000137833206020325 0.993636230056989 2176 Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication nucleoside triphosphate metabolic process nucleotide diphosphatase activity|nucleoside-triphosphate diphosphatase activity|nucleoside-triphosphatase activity|phosphodiesterase I activity cellular_component YOL048C RRT8 hom -0.000139084502963536 0.993575582921351 2177 Protein of unknown function; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|lipid particle YDR434W GPI17 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000140953052824953 0.9934892751097 2178 Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|endoplasmic reticulum|GPI-anchor transamidase complex YGL202W ARO8 hom FT KETONE METABOLISM|AMINO ACID METABOLISM -0.000141209313000827 0.993477438527548 2179 Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis aromatic amino acid family metabolic process aromatic-amino-acid:2-oxoglutarate aminotransferase activity|2-aminoadipate transaminase activity cytoplasm YBR153W RIB7 het FT OXIDATION-REDUCTION PROCESS -0.000141473580983715 0.993465232068649 2180 Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway riboflavin biosynthetic process 5-amino-6-(5-phosphoribosylamino)uracil reductase activity cellular_component YDR309C GIC2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON -0.000143651821266665 0.993370561857255 2181 Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region regulation of exit from mitosis|establishment of cell polarity|axial cellular bud site selection|Rho protein signal transduction Rho GTPase binding|Rho GTPase activator activity|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|actin cap|plasma membrane|mating projection tip|cellular bud tip YLR213C CRR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM -0.000143669290653867 0.993363812956667 2182 Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation ascospore wall assembly hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ascospore wall YLR283W_p YLR283W hom FT MITOCHONDRION -0.000144255315784312 0.993341716758157 2183 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene biological_process molecular_function mitochondrion YDL219W DTD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION -0.000144661316573011 0.993317991638764 2184 D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Unknown D-leucyl-tRNA(Leu) deacylase activity|D-tyrosyl-tRNA(Tyr) deacylase activity cytoplasm YKL131W_d YKL131W hom -0.000144897021982883 0.993307104494485 2185 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR162C TOS1 hom -0.000146805524089651 0.993226036583337 2186 Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C biological_process molecular_function fungal-type cell wall|fungal-type vacuole YOL063C CRT10 hom FT TRANSCRIPTION FROM RNA POL II -0.000147583526374895 0.993183015954999 2187 Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YDR411C DFM1 hom FT SIGNALING|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX -0.000149601500832797 0.993090837609731 2188 Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p endoplasmic reticulum unfolded protein response|ER-associated protein catabolic process molecular_function Doa10p ubiquitin ligase complex|integral to membrane|endoplasmic reticulum|Hrd1p ubiquitin ligase ERAD-L complex YCR005C CIT2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.000149697457197828 0.993089501885924 2189 Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication glyoxylate cycle|glutamate biosynthetic process|citrate metabolic process citrate (Si)-synthase activity mitochondrion|peroxisome YJL097W PHS1 het FT KETONE METABOLISM|LIPID METABOLISM|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM -0.000149936011913618 0.993074355745032 2190 Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking vacuolar transport|sphingolipid biosynthetic process enoyl-CoA hydratase activity fungal-type vacuole membrane|integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|fungal-type vacuole|nuclear outer membrane-endoplasmic reticulum membrane network YDR134C YDR134C hom -0.000152409484919317 0.99296115762996 2191 Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication biological_process molecular_function fungal-type cell wall YGR193C PDX1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION -0.000152448185906786 0.992959370319387 2192 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core acetyl-CoA biosynthetic process from pyruvate structural molecule activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YER172C BRR2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS -0.000154461443574417 0.992865328529832 2193 RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) ATP-dependent RNA helicase activity U4/U6 x U5 tri-snRNP complex|U5 snRNP YPL230W USV1 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS -0.000154956476980866 0.992842463236772 2194 Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to salt stress sequence-specific DNA binding nucleus YPR126C_d YPR126C hom -0.000155006254331187 0.992858345267404 2195 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR059C REX2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000156101708157137 0.992789565725289 2196 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease U5 snRNA 3'-end processing|U4 snRNA 3'-end processing|mitochondrial genome maintenance|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 3'-5'-exoribonuclease activity|3'-5' exonuclease activity mitochondrion YEL019C MMS21 het FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME -0.000158797009640212 0.992665071561611 2197 SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination DNA repair SUMO ligase activity nuclear envelope|Smc5-Smc6 complex|nucleus|cytoplasm YML052W SUR7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE -0.000159009938870995 0.992655236511144 2198 Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants endocytosis|ascospore formation molecular_function cell cortex|eisosome|mitochondrion|membrane raft|integral to membrane|plasma membrane|actin cortical patch YBR129C OPY1 hom FT MITOCHONDRION -0.000160501536730185 0.992586340693082 2199 Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YOR161C-C_p YOR161C-C hom -0.000161702557898097 0.992530866440756 2200 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YJR092W BUD4 hom FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON -0.000163045972433116 0.992468815190111 2201 Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p cellular bud site selection|axial cellular bud site selection GTP binding cellular bud neck contractile ring YOL086W-A MHF1 hom FT RESPONSE TO DNA DAMAGE -0.000163626179817467 0.992442015885794 2202 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 response to DNA damage stimulus molecular_function cellular_component YDL135C RDI1 hom FT MEMBRANE ORGANIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH -0.000164404832355414 0.99241058346374 2203 Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|actin filament organization|small GTPase mediated signal transduction Rho GDP-dissociation inhibitor activity cytosol|plasma membrane|cellular bud neck|cellular bud tip YNL301C RPL18B hom FT TRANSLATION|RIBOSOME -0.000166126801950583 0.992326514293556 2204 Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL114W_p YDL114W hom FT OXIDATION-REDUCTION PROCESS -0.000166948620328313 0.992293158259994 2205 Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene biological_process molecular_function integral to membrane YDL063C SYO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS -0.000168755150611826 0.992209766117449 2206 Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus ribosomal large subunit biogenesis molecular_function nucleus|cytoplasm YJL167W ERG20 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM -0.000170725260880354 0.992121177597186 2207 Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis farnesyl diphosphate biosynthetic process|ergosterol biosynthetic process|isoprenoid biosynthetic process dimethylallyltranstransferase activity|geranyltranstransferase activity endoplasmic reticulum|cytoplasm YNL045W LAP2 hom FT KETONE METABOLISM|LIPID METABOLISM|PROTEOLYSIS|NUCLEUS -0.000173722206761802 0.991975690053463 2208 Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus cellular lipid metabolic process|protein catabolic process aminopeptidase activity|leukotriene-A4 hydrolase activity|epoxide hydrolase activity nucleus|cytoplasm YKL165C MCD4 het FF|FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS -0.000173790880902491 0.991972518069761 2209 Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes GPI anchor biosynthetic process|ATP transport mannose-ethanolamine phosphotransferase activity integral to membrane|fungal-type cell wall|endoplasmic reticulum|fungal-type vacuole YJL035C TAD2 het FT RNA PROCESSING|NUCLEUS -0.000177478181775917 0.991802205849192 2210 Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YOR159C SME1 het FT RNA PROCESSING|NUCLEUS -0.000177670434414679 0.99179332592932 2211 Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YGR213C RTA1 hom FT PLASMA MEMBRANE -0.000178732652641512 0.991744263355846 2212 Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein biological_process molecular_function integral to membrane|plasma membrane YMR210W YMR210W hom FT KETONE METABOLISM|LIPID METABOLISM -0.00018080868387715 0.991648374077559 2213 Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification medium-chain fatty acid biosynthetic process transferase activity, transferring acyl groups cellular_component YPR167C MET16 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS -0.000181577248282046 0.991612875082409 2214 3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process phosphoadenylyl-sulfate reductase (thioredoxin) activity cytoplasm YMR172W HOT1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS -0.000181942408089609 0.991602279765599 2215 Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p cellular hyperosmotic salinity response|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YDR082W STN1 het FT NUCLEUS|CHROMOSOME -0.000181959755658159 0.991598969926521 2216 Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping telomere capping|negative regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YLL028W TPO1 hom FT PLASMA MEMBRANE -0.000182321827171208 0.991578483963013 2217 Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane spermidine transport|putrescine transport|spermine transport spermidine transmembrane transporter activity|spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|cellular bud membrane|plasma membrane YOR100C CRC1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION -0.000182442047096244 0.99157293116429 2218 Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation fatty acid metabolic process carnitine:acyl carnitine antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YPL190C NAB3 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS -0.000182517669128142 0.991569438283248 2219 RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing nuclear mRNA surveillance|mRNA 3'-end processing|antisense RNA transcript catabolic process|CUT catabolic process|termination of RNA polymerase II transcription, exosome-dependent|tRNA 3'-end processing|snRNA 3'-end processing|snoRNA 3'-end processing RNA binding|transcription regulatory region RNA binding Nrd1 complex|nucleus YLR393W ATP10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION -0.000182611623662433 0.991565098648536 2220 Mitochondrial inner membrane protein required for assembly