Strain Gene Zyg FClass FTerms similarity score p-value rank description GOBP GOMF GOCC YOR147W MDM32 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 1 0 1 Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 mitochondrion inheritance|cellular ion homeostasis|mitochondrion organization molecular_function mitochondrion|mitochondrial inner membrane YJL071W ARG2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.10854230864452 2.89584760227049e-10 2 Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p arginine biosynthetic process|ornithine biosynthetic process acetyl-CoA:L-glutamate N-acetyltransferase activity mitochondrial matrix YIL068C SEC6 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.107555631952975 4.21962083943911e-10 3 Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p exocyst localization|Golgi to plasma membrane transport|exocytosis|vesicle fusion|negative regulation of SNARE complex assembly|vesicle docking involved in exocytosis protein homodimerization activity mating projection tip|cellular bud neck|cellular bud tip|exocyst YOR333C_d YOR333C hom 0.106052976788074 7.32794251126668e-10 4 Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Unknown Unknown Unknown YCL040W GLK1 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.102706543454015 2.57959123836329e-09 5 Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication glucose import|mannose metabolic process|glycolysis|glucose metabolic process glucokinase activity cytosol|plasma membrane YLL033W IRC19 hom 0.100142954096251 6.1056127426222e-09 6 Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci mitotic recombination|ascospore formation molecular_function cellular_component YKL109W HAP4 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0983741836613865 1.12547555758924e-08 7 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex regulation of cellular respiration|carbon catabolite activation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity CCAAT-binding factor complex YDR512C EMI1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MITOCHONDRION ORGANIZATION 0.0961434891940966 2.39767127376465e-08 8 Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs ascospore formation|mitochondrial respiratory chain complex assembly|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YGL237C HAP2 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0945171768659993 4.17549338466756e-08 9 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex|nucleus YHR050W-A_p YHR050W-A hom 0.0944811603285614 4.16713076821198e-08 10 Protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YER071C_p TDA2 hom FT SITE OF POLARIZED GROWTH 0.0932911635129895 6.40973675682393e-08 11 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function mating projection tip|cytoplasm YCL009C ILV6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0927330439905461 7.37793370125677e-08 12 Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria branched-chain amino acid biosynthetic process enzyme regulator activity|acetolactate synthase activity mitochondrial nucleoid|acetolactate synthase complex|mitochondrion YER058W PET117 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0925722923083362 7.77188025329614e-08 13 Protein required for assembly of cytochrome c oxidase mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion YKL016C ATP7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.091874047308213 9.73237931325957e-08 14 Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis protein complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase, stator stalk|integral to membrane|mitochondrial proton-transporting ATP synthase complex YER141W COX15 hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.090654663232771 1.43591707167765e-07 15 Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase heme a biosynthetic process oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor mitochondrion|integral to membrane|mitochondrial inner membrane YJR120W YJR120W hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0897950140944624 1.92312379218953e-07 16 Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p cellular respiration|sterol transport|mitochondrion organization molecular_function cellular_component YDR510W SMT3 het FT NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0894486994867856 2.09923668890014e-07 17 Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 protein sumoylation protein tag condensed nuclear chromosome|nucleus|septin ring YLR436C ECM30 hom FT CELL WALL ORG/BIOGENESIS 0.0885691242333782 2.7607550120021e-07 18 Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization molecular_function cytoplasm YOR330C MIP1 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0885350088734525 2.84751956007489e-07 19 Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit mitochondrial DNA replication 3'-5' exonuclease activity|DNA-directed DNA polymerase activity mitochondrion YJL166W QCR8 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0879916754558164 3.30006224410728e-07 20 Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YPL148C PPT2 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0878751784814934 3.42056418730915e-07 21 Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation protein-cofactor linkage holo-[acyl-carrier-protein] synthase activity mitochondrion YLL018C-A COX19 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0877404811640325 3.57948853845798e-07 22 Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex IV assembly|metal ion transport copper ion binding|copper chaperone activity cytosol|mitochondrial intermembrane space YCL057C-A MOS1 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0864632083755312 5.26404677546398e-07 23 Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YJL127C-B_p YJL127C-B hom 0.0858005999223569 6.57925925149962e-07 24 Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii biological_process molecular_function cellular_component YJR077C MIR1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0857924154805598 6.44550051982373e-07 25 Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated phosphate ion transmembrane transport inorganic phosphate transmembrane transporter activity mitochondrion|integral to membrane|integral to mitochondrial inner membrane YMR066W SOV1 hom FT MITOCHONDRION 0.0855867240373195 6.8825353063422e-07 26 Mitochondrial protein of unknown function biological_process molecular_function mitochondrion YHL038C CBP2 hom FT RNA PROCESSING|MITOCHONDRION 0.0841964865805689 1.03721243748023e-06 27 Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Group I intron splicing RNA binding mitochondrion YDR230W_d YDR230W hom 0.0830268910017873 1.48857122812861e-06 28 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20 Unknown Unknown Unknown YNL122C_p YNL122C hom FT TRANSLATION|MITOCHONDRION 0.0822028196546956 1.87681581838941e-06 29 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene biological_process molecular_function mitochondrion YER066W_p RRT13 hom 0.0819848175293061 1.97760064432282e-06 30 Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component YOR165W SEY1 hom FT MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0817702581527594 2.10355888175163e-06 31 Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 cellular membrane organization|endoplasmic reticulum organization|endoplasmic reticulum membrane fusion|endoplasmic reticulum inheritance GTPase activity integral to membrane|endoplasmic reticulum|cortical endoplasmic reticulum|cytoplasm YOL009C MDM12 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0816708656690339 2.16448498035502e-06 32 Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins mitochondrion inheritance|mitochondrion organization|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrion|ERMES complex YPR055W SEC8 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0805885366092676 2.94758281279471e-06 33 Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Golgi inheritance|endoplasmic reticulum inheritance|Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip|exocyst|cellular bud tip YGR219W_d YGR219W hom 0.0804857766577269 3.04496900503806e-06 34 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Unknown Unknown Unknown YDR377W ATP17 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.080138585078914 3.34753979576629e-06 35 Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YAL048C GEM1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0791697921093323 4.39260762263977e-06 36 Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases mitochondrion inheritance|regulation of mitochondrion organization|phospholipid homeostasis GTPase activity|calcium ion binding mitochondrial outer membrane|ERMES complex|mitochondrion YPL078C ATP4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0790819060416899 4.50153181774155e-06 37 Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated protein complex oligomerization|ATP synthesis coupled proton transport proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase, stator stalk|integral to membrane YDR518W EUG1 hom FT ENDOPLASMIC RETICULUM 0.078473547073107 5.32960144832465e-06 38 Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER protein folding protein disulfide isomerase activity|protein disulfide oxidoreductase activity endoplasmic reticulum YGR171C MSM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0781177279538132 5.87951914681512e-06 39 Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p methionyl-tRNA aminoacylation methionine-tRNA ligase activity mitochondrion YER087W AIM10 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0780651476637079 5.9652485801345e-06 40 Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process proline-tRNA ligase activity mitochondrion YJL022W_d YJL022W hom 0.0778101675559133 6.39821978210258e-06 41 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Unknown Unknown Unknown YML081C-A ATP18 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0776132825712553 6.75294048591103e-06 42 Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YBR068C BAP2 hom FT PLASMA MEMBRANE 0.0773160648608663 7.3242609396443e-06 43 High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity integral to membrane|plasma membrane YGL233W SEC15 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.077228798393041 7.50059666150669e-06 44 Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis Rab GTPase binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YOR065W CYT1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0769924269943182 8.09895324601874e-06 45 Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex oxidative phosphorylation|mitochondrial electron transport, ubiquinol to cytochrome c electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity mitochondrion|mitochondrial respiratory chain|integral to membrane|mitochondrial inner membrane|mitochondrial respiratory chain complex III YGL064C MRH4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0769497479674013 8.09210307373895e-06 46 Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function RNA metabolic process RNA helicase activity mitochondrion YDR347W MRP1 hom FT TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0769493141292164 8.09305647499233e-06 47 Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YLR347C KAP95 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0766855765214914 8.8009151956025e-06 48 Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p protein targeting to membrane|regulation of protein desumoylation|protein import into nucleus|phosphatidylcholine biosynthetic process|nuclear pore complex assembly protein transporter activity|Ran guanyl-nucleotide exchange factor activity integral to membrane|nuclear exosome (RNase complex)|nuclear pore|cytoplasm YDL068W_d YDL068W hom 0.0763601651425588 9.49176073030677e-06 49 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR037C SCO1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.075759319351076 1.11544044518376e-05 50 Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication copper ion transport|response to redox state|protein complex assembly copper ion binding|thioredoxin peroxidase activity mitochondrion|mitochondrial inner membrane YPL199C_p YPL199C hom 0.0755091515515008 1.19256348936369e-05 51 Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cytoplasm YJL023C PET130 hom FT MITOCHONDRION 0.0754052441923221 1.22606951554548e-05 52 Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YJL209W CBP1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0753081545703869 1.27327589638821e-05 53 Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress mitochondrial mRNA catabolic process|aerobic respiration mRNA binding mitochondrion|extrinsic to mitochondrial inner membrane YDR375C BCS1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0751444354827655 1.31807702012988e-05 54 Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases protein insertion into mitochondrial membrane from inner side|chaperone-mediated protein complex assembly|mitochondrial respiratory chain complex III assembly protein transmembrane transporter activity|ATPase activity mitochondrion|mitochondrial inner membrane YCR053W THR4 hom FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|MEMBRANE ORGANIZATION|AMINO ACID METABOLISM|NUCLEUS 0.0740862253755168 1.77303712227502e-05 55 Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway endocytosis|threonine metabolic process threonine synthase activity nucleus|cytoplasm YMR075C-A_d YMR075C-A hom 0.0738789481355832 1.85033682793834e-05 56 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Unknown Unknown Unknown YMR035W IMP2 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0735621884932141 1.99255640627826e-05 57 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein processing involved in protein targeting to mitochondrion endopeptidase activity mitochondrial inner membrane peptidase complex YDR462W MRPL28 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0735004206637139 2.02486000501856e-05 58 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YKL208W CBT1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0734006266175171 2.12581354573676e-05 59 Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p cytochrome complex assembly|mRNA processing|mRNA stabilization molecular_function mitochondrion YGR112W SHY1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0732678646385499 2.15101110272545e-05 60 Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane YOR125C CAT5 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.073210415125983 2.18330761065054e-05 61 Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation ubiquinone biosynthetic process monooxygenase activity mitochondrion|mitochondrial inner membrane YHL046C PAU13 hom 0.0732034325438711 2.18726432283288e-05 62 Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock biological_process molecular_function cellular_component YMR194C-B CMC4 hom FT MITOCHONDRION 0.0731856914271004 2.19734811866524e-05 63 Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs biological_process molecular_function mitochondrial intermembrane space YKL087C CYT2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION 0.0727134108540491 2.48263114698982e-05 64 Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 cytochrome c-heme linkage holocytochrome-c synthase activity mitochondrion|mitochondrial intermembrane space YGL160W AIM14 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0725224637478508 2.61493099723033e-05 65 NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p biological_process oxidoreductase activity, oxidizing metal ions integral to membrane|ribosome YNL213C RRG9 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0722485481470724 2.79759081058325e-05 66 Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies mitochondrion organization|mitochondrial genome maintenance molecular_function mitochondrion YGL146C_p RRT6 hom 0.0715217512509672 3.36721664262339e-05 67 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins biological_process molecular_function membrane YHR116W COX23 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0712202996620494 3.64416535265061e-05 68 Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex assembly molecular_function mitochondrion|cytoplasm YGL107C RMD9 hom FT TRANSLATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0707933475191959 4.04749266152039e-05 69 Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes translational initiation|regulation of mRNA stability|aerobic respiration molecular_function extrinsic to mitochondrial inner membrane|mitochondrion YFR033C QCR6 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0706278297434488 4.21930442391099e-05 70 Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YGR187C HGH1 hom 0.0705608772421194 4.30205141676185e-05 71 Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function cytoplasm YLR361C-A_p YLR361C-A hom 0.0703851670961782 4.48374281972724e-05 72 Putative protein of unknown function biological_process molecular_function cellular_component YLR395C COX8 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0702495795547985 4.63826285857024e-05 73 Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDR237W MRPL7 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0695878995618738 5.46753938088095e-05 74 Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YHR100C GEP4 hom FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0695520650705025 5.58728497406524e-05 75 Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT cardiolipin biosynthetic process|phosphorylated carbohydrate dephosphorylation phosphatidylglycerophosphatase activity mitochondrion|mitochondrial matrix YOR298W MUM3 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS 0.0693430481985721 5.82338113145468e-05 76 Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases ascospore wall assembly|phospholipid biosynthetic process transferase activity, transferring acyl groups cellular_component YMR072W ABF2 hom FT MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0684636766089602 7.20692217624081e-05 77 Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation mitochondrion inheritance|mitochondrial DNA packaging|mitochondrial genome maintenance DNA binding, bending|DNA binding mitochondrial nucleoid|mitochondrial chromosome|mitochondrion YBL021C HAP3 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0681758936751315 7.72984622236474e-05 78 Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex YPL215W CBP3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.067484779657088 9.13589692546829e-05 79 Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex positive regulation of mitochondrial translation|mitochondrial respiratory chain complex III assembly ribosome binding integral to mitochondrial membrane|mitochondrion|mitochondrial ribosome YDR268W MSW1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.067084431100638 0.000100574583415953 80 Mitochondrial tryptophanyl-tRNA synthetase mitochondrial tryptophanyl-tRNA aminoacylation tryptophan-tRNA ligase activity mitochondrion YGR102C GTF1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0660829110770502 0.000129108280812204 81 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria glutaminyl-tRNAGln biosynthesis via transamidation glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity mitochondrion|glutamyl-tRNA(Gln) amidotransferase complex YPL132W COX11 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION|RIBOSOME 0.0660169912949481 0.000129619449309012 82 Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p protein complex assembly|aerobic respiration copper ion binding mitochondrial envelope|mitochondrion|mitochondrial ribosome|mitochondrial inner membrane YPL085W SEC16 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0659968991400351 0.00013023531419868 83 COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p COPII vesicle coating protein anchor ER to Golgi transport vesicle membrane YBR249C ARO4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0658840286356887 0.000133746570394666 84 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity nucleus|cytoplasm YKL023W_p YKL023W hom 0.0656502668529451 0.000141304063703114 85 Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YNL252C MRPL17 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0653212638169446 0.000152624053377307 86 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YEL059C-A SOM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0653023340604165 0.000153300660323485 87 Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs proteolysis|mitochondrial respiratory chain complex assembly endopeptidase activity mitochondrial inner membrane peptidase complex|mitochondrial inner membrane YIL044C AGE2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.0651823099055638 0.000158013839400957 88 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport ARF GTPase activator activity clathrin-coated vesicle YML129C COX14 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.06468518411852 0.000176969764491967 89 Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes mitochondrial respiratory chain complex IV assembly|negative regulation of mitochondrial translation molecular_function mitochondrial envelope|mitochondrion|integral to membrane|mitochondrial inner membrane|mitochondrial matrix|extrinsic to membrane YDR148C KGD2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0644820198703319 0.000187136969095836 90 Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated tricarboxylic acid cycle|mitochondrial genome maintenance|2-oxoglutarate metabolic process dihydrolipoyllysine-residue succinyltransferase activity mitochondrial nucleoid|mitochondrion|mitochondrial oxoglutarate dehydrogenase complex YPL013C MRPS16 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0644340447623452 0.000187551609548414 91 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YER036C ARB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|NUCLEUS 0.064326177027648 0.000192276747275585 92 ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p ribosomal small subunit export from nucleus|ribosome biogenesis ATPase activity, uncoupled ribosome|nucleus|cytoplasm YNL005C MRP7 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0635708363015155 0.000229126959786004 93 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome|peptidyltransferase activity mitochondrion|mitochondrial large ribosomal subunit YGR220C MRPL9 hom FT TRANSLATION|MITOCHONDRION|RIBOSOME 0.0632584986957466 0.000245474870229007 94 Mitochondrial ribosomal protein of the large subunit cytoplasmic translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YIL116W HIS5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0629463522138094 0.000265704008043674 95 Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts histidine biosynthetic process histidinol-phosphate transaminase activity intracellular YER171W RAD3 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0629338361131634 0.00026420949238487 96 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress regulation of transposition, RNA-mediated|phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair, DNA incision|regulation of mitotic recombination|transcription from RNA polymerase II promoter damaged DNA binding|ATP-dependent 5'-3' DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YFL037W TUB2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0625244193709863 0.000290353041172679 97 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|kinetochore microtubule|cytoplasmic microtubule|nuclear microtubule|spindle pole body|polar microtubule YDR116C MRPL1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0624628715738541 0.000293779818404949 98 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YGR222W PET54 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0619939784054299 0.000328296248997715 99 Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Group I intron splicing|positive regulation of mitochondrial translation|mitochondrial respiratory chain complex IV biogenesis translation regulator activity|RNA binding mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial inner membrane|mitochondrial matrix YMR218C TRS130 het FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.0617368850003519 0.000349036124576051 100 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy regulation of Rab GTPase activity|early endosome to Golgi transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex|trans-Golgi network|early endosome YJR021C REC107 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0616951574913304 0.000348708768707646 101 Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation reciprocal meiotic recombination molecular_function condensed nuclear chromosome YJL046W AIM22 hom FT MITOCHONDRION 0.0614631380542215 0.000367112261047802 102 Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss protein lipoylation lipoate-protein ligase activity mitochondrion YNR036C MRPS12 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0612507999081067 0.000389416938395976 103 Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|ribosome|mitochondrial small ribosomal subunit YLR361C DCR2 hom FT CELL CYCLE|SIGNALING 0.0611721124785772 0.000392265912506358 104 Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START endoplasmic reticulum unfolded protein response|traversing start control point of mitotic cell cycle|protein dephosphorylation hydrolase activity, acting on ester bonds|phosphoprotein phosphatase activity|phosphoric ester hydrolase activity cellular_component YGL053W PRM8 hom FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0611462404371858 0.000393717629983673 105 Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family biological_process molecular_function integral to membrane|endoplasmic reticulum|plasma membrane YNR074C AIF1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION|PLASMA MEMBRANE 0.0611080126604683 0.000397046082105567 106 Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase response to singlet oxygen|positive regulation of apoptotic process oxidoreductase activity, acting on a sulfur group of donors, NAD or NADP as acceptor mitochondrion|nucleus|plasma membrane YHR061C GIC1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0607939026910322 0.000425407342634456 107 Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress regulation of exit from mitosis|establishment of cell polarity|axial cellular bud site selection|Rho protein signal transduction Rho GTPase binding|Rho GTPase activator activity incipient cellular bud site|actin cap|mating projection tip|cellular bud neck|cellular bud tip YDR539W FDC1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|PLASMA MEMBRANE 0.06065860526852 0.000438196798742861 108 Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm cinnamic acid catabolic process molecular_function cytoplasm YJL019W MPS3 het FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0604432848034251 0.000460194456858165 109 Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p meiotic telomere clustering|synapsis|nuclear migration involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope|telomere tethering at nuclear periphery|mitotic sister chromatid cohesion molecular_function nuclear envelope|nuclear periphery|integral to membrane|nuclear chromosome, telomeric region|half bridge of spindle pole body YLR262C-A TMA7 hom FT TRANSLATION|NUCLEUS 0.0604222080941235 0.000462313121159468 110 Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress cytoplasmic translation molecular_function nucleus|cytoplasm YIR022W SEC11 het FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0597587900353985 0.000532816148698446 111 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex|endoplasmic reticulum YKR073C_d YKR073C hom 0.0594413931623229 0.000570504786123782 112 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR185C MBA1 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0594263199566414 0.000572354909243012 113 Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane protein insertion into mitochondrial membrane from inner side|mitochondrial respiratory chain complex III biogenesis|inner mitochondrial membrane organization|positive regulation of mitochondrial translation|mitochondrial respiratory chain complex IV biogenesis ribosome binding mitochondrion|mitochondrial inner membrane YLR429W CRN1 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0594180109355238 0.00057337716549775 114 Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly microtubule-based process|actin filament organization|actin cortical patch localization|Unknown|negative regulation of Arp2/3 complex-mediated actin nucleation microtubule binding|actin filament binding|protein binding, bridging Arp2/3 protein complex|actin cortical patch YJL062W-A COA3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0593710960037139 0.000579181051387309 115 Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly mitochondrial respiratory chain complex IV assembly|negative regulation of mitochondrial translation molecular_function mitochondrion|mitochondrial inner membrane|integral to mitochondrial inner membrane YDR405W MRP20 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0587744638453794 0.000657919996191066 116 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YCR024C SLM5 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0584404216106024 0.00070623936278247 117 Mitochondrial asparaginyl-tRNA synthetase asparaginyl-tRNA aminoacylation|mitochondrial asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity mitochondrion YPR195C_d YPR195C hom 0.0584133469172289 0.000710296748216274 118 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL052W COX5A hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0582422292175047 0.000736445206259832 119 Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YIL123W SIM1 hom FT CELL WALL ORG/BIOGENESIS 0.0578931400977878 0.000792583186927575 120 Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated fungal-type cell wall organization molecular_function fungal-type cell wall YBR102C EXO84 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|SITE OF POLARIZED GROWTH 0.0576909636041619 0.000826885450904573 121 Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate spliceosomal complex assembly|Golgi to plasma membrane transport|exocyst localization|exocytosis|exocyst assembly molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip|exocyst YOR028C CIN5 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0575287441809925 0.000855398343218225 122 Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication hyperosmotic salinity response|response to drug|hyperosmotic response|regulation of transcription from RNA polymerase II promoter repressing transcription factor binding|sequence-specific DNA binding nucleus|cytoplasm YBR019C GAL10 hom FT CARBOHYDRATE METABOLISM 0.0574082687635127 0.000877160830253627 123 UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers galactose catabolic process via UDP-galactose aldose 1-epimerase activity|UDP-glucose 4-epimerase activity cytosol YBR282W MRPL27 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0573544383148148 0.000887049565184345 124 Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YDL142C CRD1 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.05734827315142 0.000888188683944144 125 Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis lipid biosynthetic process|cellular ion homeostasis|mitochondrial membrane organization cardiolipin synthase activity mitochondrion|integral to membrane YEL014C_d YEL014C hom 0.0571242291872612 0.000930515949881138 126 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL020C ULP1 het FT CELL CYCLE|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0569935984536036 0.000956049642472623 127 Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions G2/M transition of mitotic cell cycle|protein desumoylation cysteine-type peptidase activity|SUMO-specific protease activity nuclear envelope|nucleolus|nuclear pore YOR191W ULS1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0568796103147279 0.000978858687263321 128 Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress chromatin assembly or disassembly|mating type switching SUMO binding|DNA-dependent ATPase activity mitochondrion|nucleolus|nucleus YNR045W PET494 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0566433106436196 0.00102775518171812 129 Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane mitochondrial respiratory chain complex IV biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrion|mitochondrial inner membrane YLR079W SIC1 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0563807245868166 0.00108473568281216 130 Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 regulation of cyclin-dependent protein kinase activity|negative regulation of macroautophagy|G1/S transition of mitotic cell cycle cyclin-dependent protein kinase inhibitor activity nucleus|cytoplasm YMR009W ADI1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0563450679491819 0.00109269444870295 131 Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions L-methionine salvage from methylthioadenosine oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen nucleus|cytoplasm YGR174W-A_p YGR174W-A hom 0.0562817744732325 0.00112032915453875 132 Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis biological_process molecular_function cellular_component YER062C HOR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0562742217019608 0.00110866814950042 133 Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication cellular carbohydrate metabolic process|glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YNR054C ESF2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0561874374805556 0.00112852944808351 134 Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|small-subunit processome assembly|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATPase activator activity|RNA binding nucleolus|90S preribosome|cytoplasm YPL138C SPP1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0560061468933147 0.00117108444265174 135 Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein histone H3-K4 methylation|regulation of transcription, DNA-dependent|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific)|chromatin binding|methylated histone residue binding Set1C/COMPASS complex YER061C CEM1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0559467258886809 0.00118535192008291 136 Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration fatty acid biosynthetic process fatty acid synthase activity mitochondrion YMR089C YTA12 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0559110171446032 0.00119400293292608 137 Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes proteolysis|signal peptide processing|protein complex assembly|protein import into mitochondrial intermembrane space metallopeptidase activity|protein binding|ATPase activity|ATP binding mitochondrion|mitochondrial inner boundary membrane|mitochondrial inner membrane|m-AAA complex|cytoplasm YJL148W RPA34 hom FT NUCLEUS 0.0558672719182942 0.00120468036770691 138 RNA polymerase I subunit A34.5 transcription elongation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter molecular_function DNA-directed RNA polymerase I complex|nucleolus|nucleus YDR197W CBS2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0558586051004754 0.00120680622049414 139 Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial respiratory chain complex III biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial ribosome YNR067C DSE4 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION 0.0557702511469285 0.00122867676420418 140 Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother cytokinesis, completion of separation glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall|extracellular region|cell septum YOR205C GEP3 hom FT MITOCHONDRION 0.0556342476737154 0.00126518044543494 141 Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) maturation of SSU-rRNA molecular_function mitochondrion|mitochondrial inner membrane YDR507C GIN4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.0554606327867422 0.00130823773552894 142 Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication protein autophosphorylation|budding cell bud growth|protein phosphorylation|septin checkpoint|septin ring assembly protein kinase activity cellular bud neck YLR316C TAD3 het FT RNA PROCESSING|NUCLEUS 0.0552464972398832 0.00136601418985697 143 Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YGR121W-A_p YGR121W-A hom 0.0552258938554982 0.001371695542067 144 Putative protein of unknown function biological_process molecular_function cellular_component YJR020W_d YJR020W hom 0.0551688669268065 0.00138753441615526 145 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR094W_d YDR094W hom 0.055051506300113 0.00142066238543201 146 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Unknown Unknown Unknown YIL045W PIG2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0549973938627864 0.00143618115715621 147 Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase regulation of glycogen biosynthetic process protein phosphatase type 1 regulator activity cytoplasm YJR011C_p YJR011C hom 0.0549394243367235 0.00145297927266381 148 Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YDR524C-B_p YDR524C-B hom FT PLASMA MEMBRANE 0.0548212206538971 0.00148779392830779 149 Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YGR178C PBP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|NUCLEUS|MITOCHONDRION 0.0547083549777839 0.00152175181685923 150 Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress positive regulation of translation|mRNA polyadenylation|stress granule assembly molecular_function mitochondrion|nucleus|polysome|cytoplasmic stress granule|cytoplasm YDR079W PET100 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0546839469597349 0.0015291886769811 151 Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|mitochondrial inner membrane|integral to mitochondrial inner membrane YBR003W COQ1 hom FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0546466462113424 0.0015406184737452 152 Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis ubiquinone biosynthetic process trans-hexaprenyltranstransferase activity mitochondrion YBL009W ALK2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION 0.054640678869831 0.00154245428297276 153 Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins meiosis|mitosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity cellular_component YHR120W MSH1 hom FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0545372837314462 0.00157458446744835 154 DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis DNA repair|mismatch repair|mitochondrial DNA repair|mitochondrion organization|reciprocal meiotic recombination|mitochondrial genome maintenance guanine/thymine mispair binding|dinucleotide insertion or deletion binding|DNA-dependent ATPase activity mitochondrion YLR270W DCS1 hom FT NUCLEUS|MITOCHONDRION 0.0545223669481478 0.00157927038926837 155 Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|positive regulation of nuclease activity|nuclear-transcribed mRNA catabolic process, deadenylation-independent decay hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides|enzyme activator activity|m7G(5')pppN diphosphatase activity mitochondrion|nucleus|cytoplasmic mRNA processing body|cytoplasm YBR251W MRPS5 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0544461166192161 0.00160342457574756 156 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YJL005W CYR1 het FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0543623700831239 0.00163034472808461 157 Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation adenylate cyclase-modulating G-protein coupled receptor signaling pathway|Ras protein signal transduction adenylate cyclase activity mitochondrion|plasma membrane YOL013C HRD1 hom FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0542724507104227 0.00165971151825037 158 Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon fungal-type cell wall organization|ER-associated protein catabolic process ubiquitin-protein ligase activity Hrd1p ubiquitin ligase ERAD-M complex|integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YER166W DNF1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE 0.0540630699001781 0.00172998818547363 159 Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YER008C SEC3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0539432611582014 0.00177986429042315 160 Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress exocytosis|Golgi to plasma membrane transport|endoplasmic reticulum inheritance|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|exocyst|cellular bud tip YOR083W WHI5 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0537844142621053 0.00182776026908477 161 Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1 negative regulation of transcription involved in G1/S phase of mitotic cell cycle|regulation of cell size RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription SBF transcription complex|nucleus|cytoplasm YGR183C QCR9 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0537402567278358 0.00185535607086434 162 Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex iron-sulfur cluster assembly|mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YPR060C ARO7 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0536539260252656 0.00187526763325418 163 Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis aromatic amino acid family biosynthetic process chorismate mutase activity nucleus|cytoplasm YMR184W ADD37 hom FT PROTEOLYSIS 0.0536091712593901 0.0018918210358582 164 Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress ER-associated protein catabolic process molecular_function cytoplasm YHR117W TOM71 hom FT MITOCHONDRION 0.0533840690375497 0.00197713198050902 165 Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication biological_process protein transporter activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane YJL043W_p YJL043W hom FT MITOCHONDRION 0.0532730898098618 0.00202047808009314 166 Putative protein of unknown function; YJL043W is a non-essential gene biological_process molecular_function mitochondrion|cytoplasm YBL068W PRS4 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0532660684554699 0.00202324957276878 167 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YAL004W_d YAL004W hom 0.0532225417856646 0.00205952348239949 168 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Unknown Unknown Unknown YOR184W SER1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0532080685358854 0.0020685302217137 169 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress serine family amino acid biosynthetic process|L-serine biosynthetic process|purine nucleobase biosynthetic process O-phospho-L-serine:2-oxoglutarate aminotransferase activity cytoplasm YLR069C MEF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0531059509553706 0.00208740579591585 170 Mitochondrial elongation factor involved in translational elongation mitochondrial translational elongation|mitochondrial translation translation elongation factor activity mitochondrion YDR322C-A TIM11 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0530338917386018 0.00212992667410372 171 Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase protein oligomerization|cristae formation|ATP synthesis coupled proton transport structural molecule activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YMR267W PPA2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0529024503589156 0.00217828512029309 172 Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate aerobic respiration inorganic diphosphatase activity mitochondrion YFR024C-A LSB3 hom FT MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0527553264367734 0.00223445830344471 173 Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication actin cortical patch localization molecular_function mitochondrion|cellular bud neck|cytoplasm YAL067W-A_p YAL067W-A hom 0.0526687007350434 0.00227566969264206 174 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YLR038C COX12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0525391221301012 0.00232980284771221 175 Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV mitochondrial respiratory chain complex IV assembly cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YKL118W_d YKL118W hom FF 0.0524062439327904 0.00240103007896547 176 Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Unknown Unknown Unknown YPL149W ATG5 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0523765448799519 0.0024039355973712 177 Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation C-terminal protein lipidation|mitochondrion degradation|piecemeal microautophagy of nucleus|macroautophagy|CVT pathway Atg8 ligase activity cytosol|Atg12-Atg5-Atg16 complex|pre-autophagosomal structure|autophagic vacuole YOR309C_d YOR309C hom 0.0522292619208948 0.00247295111890554 178 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Unknown Unknown Unknown YJL200C ACO2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0521444837718512 0.00252850245186863 179 Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol biological_process aconitate hydratase activity mitochondrion YGL116W CDC20 het FF|FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.05214337562867 0.00251402844185729 180 Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress spindle checkpoint|activation of mitotic anaphase-promoting complex activity|meiotic anaphase I|positive regulation of protein catabolic process|activation of anaphase-promoting complex activity involved in meiotic cell cycle|positive regulation of mitotic metaphase/anaphase transition|cyclin catabolic process anaphase-promoting complex binding|ubiquitin-protein ligase activator activity mitotic checkpoint complex|anaphase-promoting complex YLR287C-A RPS30A hom FT TRANSLATION|RIBOSOME 0.051833094331826 0.00266768048889554 181 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasmic stress granule YPL100W ATG21 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.0516661422209163 0.00275386061021411 182 Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein piecemeal microautophagy of nucleus|vesicle organization|mitochondrion degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway|protein lipidation phosphatidylinositol binding endosome|cytosol|fungal-type vacuole membrane YCR008W SAT4 hom FT PROTEIN LOCALIZATION|CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0515567968576445 0.00281167409256269 183 Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p cellular cation homeostasis|protein phosphorylation|cellular protein localization|G1/S transition of mitotic cell cycle|protein dephosphorylation protein kinase activity cellular_component YER068W MOT2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0514808375192209 0.00285248540244625 184 Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|protein polyubiquitination|protein ubiquitination ubiquitin-protein ligase activity|sequence-specific DNA binding CCR4-NOT core complex|cytoplasm YOL079W_d YOL079W hom 0.0513956548985692 0.00289889471167968 185 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER052C HOM3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0513245520311212 0.00295519424117777 186 Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis threonine biosynthetic process|methionine biosynthetic process|homoserine biosynthetic process aspartate kinase activity cytoplasm YHR194W MDM31 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|NUCLEUS|MITOCHONDRION 0.0512783072158095 0.00296395668022104 187 Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 mitochondrion inheritance|cellular ion homeostasis|mitochondrion organization|Unknown molecular_function mitochondrial inner membrane|nucleus YLR350W ORM2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0512337216248186 0.00298902375895763 188 Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress cellular sphingolipid homeostasis|negative regulation of sphingolipid biosynthetic process|response to unfolded protein molecular_function integral to membrane|endoplasmic reticulum|SPOTS complex YIL061C SNP1 het FT RNA PROCESSING|NUCLEUS 0.0511090526900228 0.00306014507962606 189 Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding|U1 snRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YEL049W PAU2 hom 0.0510946280623207 0.00306847286855175 190 Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YMR257C PET111 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0509882664606922 0.0031305193325416 191 Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane mitochondrial respiratory chain complex IV biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrion|mitochondrial inner membrane YOR292C_p YOR292C hom 0.0509658301195289 0.0031437528000459 192 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene biological_process molecular_function integral to membrane|fungal-type vacuole YOR297C TIM18 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0507269996964637 0.00328781324064244 193 Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex cellular response to oxidative stress|protein import into mitochondrial inner membrane|apoptotic process|response to acid|response to osmotic stress|response to arsenic-containing substance protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YAL060W BDH1 hom FT OXIDATION-REDUCTION PROCESS 0.0504647626826149 0.0034528956191023 194 NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source alcohol metabolic process|butanediol biosynthetic process (R,R)-butanediol dehydrogenase activity cytoplasm YBR021W FUR4 hom FF|FT PLASMA MEMBRANE 0.0504150180614071 0.00350949994587082 195 Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation uracil transport|transmembrane transport uracil:cation symporter activity integral to membrane|membrane raft|plasma membrane YNL240C NAR1 het FT COFACTOR METABOLISM|NUCLEUS 0.050376175217839 0.00351034448249091 196 Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf iron-sulfur cluster assembly iron-sulfur cluster binding|ferredoxin hydrogenase activity cytosol|membrane YLR203C MSS51 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0503398675915851 0.00353414007328646 197 Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis mitochondrial respiratory chain complex IV biogenesis|positive regulation of mitochondrial translation translation regulator activity extrinsic to mitochondrial inner membrane|mitochondrion YBR057C MUM2 hom FT CELL CYCLE 0.0502831332705013 0.00357161690342905 198 Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex premeiotic DNA replication molecular_function cytoplasm YOL027C MDM38 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|TRANSLATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0502691621476526 0.0035809010470669 199 Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome potassium ion transport|mitochondrial respiratory chain complex III biogenesis|cellular potassium ion homeostasis|proton transport|positive regulation of mitochondrial translation|mitochondrial respiratory chain complex IV biogenesis|protein insertion into mitochondrial membrane ribosome binding mitochondrion|mitochondrial inner membrane YBR182C-A_p YBR182C-A hom 0.0501658435118516 0.00367561905842788 200 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YGR174C CBP4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0501243312660429 0.00368353778501738 201 Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|integral to mitochondrial membrane YIL007C NAS2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0499687057477554 0.00378594652569198 202 Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|cytoplasm YIL019W FAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0498959278420044 0.0038424593261214 203 Protein required for pre-rRNA processing and 40S ribosomal subunit assembly maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|cytoplasm YER088C DOT6 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0498498634192537 0.00386995205796812 204 Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress chromatin silencing at rDNA|filamentous growth|regulation of transcription from RNA polymerase II promoter|unidimensional cell growth|chromatin silencing at telomere sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|Rpd3L complex|cytoplasm YHR168W MTG2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0497721512816225 0.00393116008019645 205 Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly cytoplasmic translation ribosome binding|GTPase activity mitochondrial inner membrane|extrinsic to membrane YGL218W_d YGL218W hom 0.0497662622609735 0.00395697741204502 206 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YDR488C PAC11 hom FT CYTOSKELETON 0.0497439100797731 0.00394627015496856 207 Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 nuclear migration along microtubule|microtubule-based process plus-end-directed microtubule motor activity|microtubule motor activity cytoplasmic dynein complex|cytoplasmic microtubule YGL074C_d YGL074C het 0.0497244524386575 0.00396043305878024 208 Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Unknown Unknown Unknown YDR050C TPI1 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0495135630285352 0.00412249470166473 209 Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease glycolysis triose-phosphate isomerase activity mitochondrion|plasma membrane|cytoplasm YOL096C COQ3 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.049413511974415 0.00419310928328565 210 O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein ubiquinone biosynthetic process hexaprenyldihydroxybenzoate methyltransferase activity mitochondrion|mitochondrial inner membrane|extrinsic to membrane YDR204W COQ4 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.049232878496006 0.00433390195695822 211 Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex ubiquinone biosynthetic process molecular_function mitochondrial inner membrane YEL017C-A PMP2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0490496595750786 0.00453500882678989 212 Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication cation transport molecular_function membrane YMR071C TVP18 hom FF|FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0490361059823787 0.00451007853582665 213 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|clathrin-coated vesicle YIL070C MAM33 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0490128921017866 0.00451114643705253 214 Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R aerobic respiration molecular_function mitochondrion|mitochondrial matrix YMR293C HER2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0489944040012143 0.00455645966468801 215 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase endoplasmic reticulum organization|glutaminyl-tRNAGln biosynthesis via transamidation glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity mitochondrion|glutamyl-tRNA(Gln) amidotransferase complex YBR188C NTC20 hom FT RNA PROCESSING|NUCLEUS 0.0489918397648207 0.00452844799233701 216 Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YLR180W SAM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM 0.048936491299461 0.00457422233018845 217 S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cytoplasm YOR044W IRC23 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0489330533264327 0.00457707937590337 218 Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication biological_process molecular_function endoplasmic reticulum YJL030W MAD2 hom FT CELL CYCLE|CELL DIVISION|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|NUCLEAR PORE|KINETOCHORE 0.0488832761463527 0.00461862693787675 219 Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I chromosome decondensation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex|condensed nuclear chromosome kinetochore|nuclear pore YLR218C COA4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION 0.0488746232645772 0.00462588397625054 220 Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors mitochondrial respiratory chain complex IV assembly|mitochondrial respiratory chain complex assembly molecular_function nucleus|mitochondrial intermembrane space|cytoplasm YDR533C HSP31 hom FT PROTEOLYSIS 0.0488690249060664 0.00463058473046079 221 Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress biological_process unfolded protein binding|cysteine-type peptidase activity cellular_component YHR013C ARD1 hom FT CHROMATIN ORGANIZATION|RIBOSOME 0.048570677014962 0.00488744010868306 222 Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress histone acetylation|N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity cytosolic ribosome|NatA complex YIL135C VHS2 hom FT CELL CYCLE 0.0484742433957769 0.00497317643321453 223 Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p G1/S transition of mitotic cell cycle molecular_function cytoplasm YDR110W FOB1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0484602098995828 0.00498576588875395 224 Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases extrachromosomal circular DNA accumulation involved in cell aging|negative regulation of DNA replication|rDNA condensation|chromatin silencing at rDNA|replicative cell aging|protein localization to nucleolar rDNA repeats|DNA recombination rDNA spacer replication fork barrier binding nucleolus|rDNA heterochromatin YGL161C YIP5 hom FT GOLGI APPARATUS 0.0482699733427926 0.00515929232435203 225 Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process Rab GTPase binding integral to membrane|Golgi apparatus YJL077C ICS3 hom 0.0482302694197788 0.0052028904716079 226 Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 biological_process molecular_function cellular_component YPR159C-A_p YPR159C-A hom 0.0478721126204289 0.00553995575824247 227 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YLL057C JLP1 hom FT OXIDATION-REDUCTION PROCESS 0.047806850163188 0.00560476143304495 228 Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation sulfur compound catabolic process sulfonate dioxygenase activity cellular_component YHR131C_p YHR131C hom 0.0477686926674188 0.00568600453214717 229 Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study biological_process molecular_function cytoplasm YDR134C YDR134C hom 0.0477295682565855 0.00568957481057432 230 Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication biological_process molecular_function fungal-type cell wall YIL105C SLM1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.0476220710201562 0.00579197180731501 231 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication eisosome assembly|TOR signaling cascade|regulation of cell growth|actin filament bundle assembly|endosomal transport|establishment or maintenance of actin cytoskeleton polarity|actin cytoskeleton organization phosphatidylinositol-4,5-bisphosphate binding|phosphatidylinositol binding|sphingolipid binding eisosome|mitochondrion|membrane raft|plasma membrane|cytoplasm YMR065W KAR5 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0475301646994401 0.00588717011038801 232 Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone karyogamy involved in conjugation with cellular fusion molecular_function mitochondrion|endoplasmic reticulum membrane YNR068C_p YNR068C hom 0.0474987228387517 0.00592006038290019 233 Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form biological_process molecular_function cellular_component YBR268W MRPL37 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0474850641396665 0.00594183701445011 234 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLL009C COX17 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0473967674289406 0.00602785422204505 235 Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex IV assembly|intracellular copper ion transport copper chaperone activity cytosol|mitochondrion|mitochondrial intermembrane space YDL107W MSS2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0473663481063946 0.00606035608424886 236 Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p protein insertion into mitochondrial membrane from inner side molecular_function extrinsic to mitochondrial inner membrane|mitochondrial matrix YBR106W PHO88 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0473500315925248 0.0060778545861901 237 Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations phosphate ion transport molecular_function mitochondrion|membrane YNR064C YNR064C hom 0.047240203108765 0.0061968275691296 238 Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides response to toxin epoxide hydrolase activity cellular_component YMR277W FCP1 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0471982177001893 0.00625059714093635 239 Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) dephosphorylation of RNA polymerase II C-terminal domain|regulation of transcription from RNA polymerase II promoter protein serine/threonine phosphatase activity nucleus YOR358W HAP5 hom FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0471968986484614 0.00624431089063748 240 Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex YER148W SPT15 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0471890083212904 0.00626074501529198 241 TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability transposon integration|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly RNA polymerase III regulatory region DNA binding|RNA polymerase I transcription factor recruiting transcription factor activity|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding|DNA binding, bending|sequence-specific DNA binding|RNA polymerase II core promoter sequence-specific DNA binding|TFIIIB-type transcription factor activity|chromatin binding|RNA polymerase I transcription factor binding transcription factor TFIIIB complex|RNA polymerase I upstream activating factor complex|nucleus|RNA polymerase I core factor complex|transcription factor TFIID complex YKL194C MST1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0468397775931243 0.00664854848593545 242 Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro mitochondrial threonyl-tRNA aminoacylation threonine-tRNA ligase activity mitochondrion YOL025W LAG2 hom FT MITOCHONDRION 0.0467775540889491 0.00672133961903185 243 Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 replicative cell aging|negative regulation of ubiquitin-protein ligase activity molecular_function mitochondrion YLR201C COQ9 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0466486365237109 0.0068744346080024 244 Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes ubiquinone biosynthetic process|aerobic respiration molecular_function mitochondrion|mitochondrial inner membrane YBR109C CMD1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0464979575037272 0.00705733603656264 245 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin karyogamy involved in conjugation with cellular fusion|phosphatidylinositol biosynthetic process|NLS-bearing substrate import into nucleus|microautophagy|cell budding|vacuole fusion, non-autophagic|receptor-mediated endocytosis|cytoskeleton organization|spindle pole body organization|transcription factor import into nucleus calcium-dependent protein binding|protein binding|calcium ion binding incipient cellular bud site|central plaque of spindle pole body|mating projection tip|cellular bud neck|cellular bud tip YJR139C HOM6 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0464429215008673 0.00721144020427439 246 Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions threonine biosynthetic process|methionine biosynthetic process|homoserine biosynthetic process homoserine dehydrogenase activity nucleus|cytoplasm YOR137C SIA1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0463219911660383 0.00731137439369869 247 Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose proton transport molecular_function cellular_component YKL138C MRPL31 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0462547800798529 0.00740581616427793 248 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrial large ribosomal subunit YHR067W HTD2 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0461676007027829 0.00747370666363436 249 Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology fatty acid biosynthetic process 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity mitochondrion YHR101C BIG1 het FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0461636714759048 0.00747878872863938 250 Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan fungal-type cell wall biogenesis molecular_function integral to endoplasmic reticulum membrane YDR274C_d YDR274C hom 0.045795635764583 0.00803442544345209 251 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL031C PHO85 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES A 0.0456517667926555 0.0081679816171785 252 Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle regulation of transcription involved in G1/S phase of mitotic cell cycle|negative regulation of calcium-mediated signaling|regulation of protein stability|regulation of protein localization|protein phosphorylation|response to DNA damage stimulus|negative regulation of phosphate metabolic process|fungal-type cell wall organization|regulation of establishment or maintenance of cell polarity|negative regulation of glycogen biosynthetic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity|positive regulation of macroautophagy|negative regulation of macroautophagy protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YKL155C RSM22 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0454760690069644 0.00844658630470248 253 Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase mitochondrial translation structural constituent of ribosome|S-adenosylmethionine-dependent methyltransferase activity mitochondrion|mitochondrial small ribosomal subunit YOR200W_d YOR200W hom 0.0454649662487647 0.00843329124290142 254 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Unknown Unknown Unknown YMR151W_d YIM2 hom 0.0453858612671652 0.00854794581153163 255 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Unknown Unknown Unknown YDR383C NKP1 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0453320745993517 0.00862669765972645 256 Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 biological_process molecular_function kinetochore YIR021W MRS1 hom FT RNA PROCESSING|MITOCHONDRION 0.0452930500845341 0.00873427264183324 257 Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Group I intron splicing endodeoxyribonuclease activity|RNA binding mitochondrion YGL144C ROG1 hom FT LIPID METABOLISM 0.0452421601024375 0.00875979460640633 258 Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication cellular lipid metabolic process lipase activity cellular_component YJR048W CYC1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0450292050237665 0.00908236680496833 259 Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen|mitochondrial electron transport, ubiquinol to cytochrome c electron carrier activity mitochondrion|mitochondrial intermembrane space YMR003W AIM34 hom FT MITOCHONDRION 0.0449397878691354 0.00922094150611435 260 Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YIL132C CSM2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS 0.0449117834606132 0.00926472768020885 261 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis meiotic chromosome segregation|recombinational repair|maintenance of rDNA|error-free translesion synthesis molecular_function nucleus|Shu complex|cytoplasm YBR287W_p YBR287W hom FT ENDOPLASMIC RETICULUM 0.0448959775818241 0.00928952268619621 262 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YBL039W-B_p YBL039W-B hom 0.0448858795330926 0.00930539467052757 263 Putative protein of unknown function biological_process molecular_function cellular_component YIR026C YVH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|SIGNALING|CELL WALL ORG/BIOGENESIS|NUCLEUS 0.0448407678878242 0.00937659633949233 264 Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases meiosis|ascospore wall assembly|ribosomal large subunit assembly|cAMP-mediated signaling|protein dephosphorylation protein tyrosine phosphatase activity|protein tyrosine/serine/threonine phosphatase activity nucleus|preribosome, large subunit precursor|cytoplasm YML018C YML018C hom 0.0447764853663838 0.00953237491623048 265 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication biological_process molecular_function fungal-type vacuole membrane|integral to membrane YBR035C PDX3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.0446265098619271 0.00972144483018583 266 Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism fatty acid metabolic process pyridoxamine-phosphate oxidase activity cellular_component YLR249W YEF3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.0445262522602995 0.00988665595983161 267 Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication translational elongation|translational termination ATPase activity|translation elongation factor activity cytosolic ribosome|ribosome YFL034W_p YFL034W hom 0.0444763122005013 0.0100031803024036 268 Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk biological_process molecular_function integral to membrane YDL044C MTF2 hom FT RNA PROCESSING|TRANSLATION|MITOCHONDRION 0.0444267167302005 0.010053145967203 269 Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription cytoplasmic translation|mRNA processing RNA binding mitochondrion YDR231C COX20 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0444128929658551 0.0100764648522318 270 Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase mitochondrial respiratory chain complex IV assembly|protein processing|aerobic respiration unfolded protein binding mitochondrion|mitochondrial inner membrane YGR015C_p YGR015C hom FT MITOCHONDRION 0.0442665065431159 0.0103263698670034 271 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion YDL157C_p YDL157C hom FT MITOCHONDRION 0.0442514786545795 0.0103523343620217 272 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YKL167C MRP49 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0442259166766402 0.0103966325934984 273 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YJL180C ATP12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0442154910503683 0.0104262074898993 274 Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency mitochondrial proton-transporting ATP synthase complex assembly protein domain specific binding mitochondrion YMR158W MRPS8 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0441823962771904 0.0104724402706635 275 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YLR369W SSQ1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0440670482336939 0.0106757468339947 276 Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia iron-sulfur cluster assembly|intracellular sequestering of iron ion|protein maturation unfolded protein binding mitochondrion|mitochondrial matrix YEL003W GIM4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.0439811517731696 0.0109361007318401 277 Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YMR098C ATP25 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0439690105362376 0.0109581408332595 278 Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene mRNA stabilization|mitochondrial proton-transporting ATP synthase complex assembly molecular_function mitochondrion|mitochondrial inner membrane YJR005C-A_p YJR005C-A hom 0.0439619139412413 0.0108640991731247 279 Putative protein of unknown function; originally identified as a syntenic homolog of an Ashbya gossypii gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YDR332W IRC3 hom FT MITOCHONDRION 0.0439348045945461 0.0109131429552527 280 Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process ATP-dependent RNA helicase activity mitochondrion YJR080C AIM24 hom FT MITOCHONDRION 0.0439308454173262 0.0109203219759178 281 Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YNR027W BUD17 hom FT CELL CYCLE|CELL DIVISION|COFACTOR METABOLISM|NUCLEUS 0.0438693560831391 0.0110323579412785 282 Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) pyridoxal phosphate biosynthetic process|cellular bud site selection pyridoxal kinase activity nucleus|cytoplasm YMR150C IMP1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0437296199724114 0.0112907662426958 283 Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p protein processing involved in protein targeting to mitochondrion endopeptidase activity mitochondrion|mitochondrial inner membrane peptidase complex YLR461W PAU4 hom 0.043642122540866 0.0114552935559644 284 Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YNR041C COQ2 hom FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.043574564664995 0.0115837787651779 285 Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis ubiquinone biosynthetic process 4-hydroxybenzoate octaprenyltransferase activity mitochondrion|integral to mitochondrial membrane|integral to membrane YPR068C HOS1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS 0.0435161198911254 0.0116959616710052 286 Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex peptidyl-lysine deacetylation|histone H4 deacetylation|positive regulation of transcription from RNA polymerase II promoter histone deacetylase activity|protein deacetylase activity histone deacetylase complex YHR086W NAM8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0435054963109791 0.011716456451845 287 RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function mRNA splicing, via spliceosome|positive regulation of mRNA splicing, via spliceosome|mRNA splice site selection mRNA binding|RNA binding commitment complex|U1 snRNP|U2-type prespliceosome YJL047C-A_p YJL047C-A hom 0.0433686655259276 0.0119832879334194 288 Putative protein of unknown function biological_process molecular_function cellular_component YER122C GLO3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0431998823668687 0.0123328338312154 289 ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p retrograde vesicle-mediated transport, Golgi to ER|COPI coating of Golgi vesicle|ER to Golgi vesicle-mediated transport ARF GTPase activator activity COPI-coated vesicle|endoplasmic reticulum-Golgi intermediate compartment|COPI vesicle coat YKL170W MRPL38 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0431916186730365 0.0123365280541091 290 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YCL064C CHA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0431846525725188 0.0123506132558551 291 Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine threonine catabolic process|L-serine catabolic process L-threonine ammonia-lyase activity|L-serine ammonia-lyase activity mitochondrial nucleoid|mitochondrion YDL032W_d YDL032W hom 0.0431831609403108 0.0123536311321296 292 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Unknown Unknown Unknown YJR013W GPI14 het FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0430944236093198 0.0125343437551582 293 Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M fungal-type cell wall organization|GPI anchor biosynthetic process mannosyltransferase activity integral to membrane|endoplasmic reticulum membrane YBR200W-A_p YBR200W-A hom 0.0430032697603072 0.0127224098098975 294 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YNL081C SWS2 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0428847862949478 0.0129705904681286 295 Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency regulation of sporulation resulting in formation of a cellular spore|mitochondrial translation structural constituent of ribosome mitochondrial small ribosomal subunit|cytoplasm YIL022W TIM44 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0428699709364382 0.0130019225384291 296 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) protein import into mitochondrial matrix protein binding, bridging|chaperone binding mitochondrion|integral to membrane|presequence translocase-associated import motor YPR178W PRP4 het FT RNA PROCESSING|NUCLEUS 0.0428584980420993 0.0130669120873255 297 Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YJL116C NCA3 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0428553581498536 0.0130328917348858 298 Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication mitochondrion organization molecular_function cellular_component YMR064W AEP1 hom FT TRANSLATION|MITOCHONDRION 0.0428316114425183 0.013083357760957 299 Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase regulation of translation translation regulator activity mitochondrion YBR186W PCH2 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.042784576869432 0.0131974916401567 300 Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation meiotic recombination checkpoint|meiotic DNA double-strand break formation|reciprocal meiotic recombination ATPase activity nucleolus YOL019W_p YOL019W hom FT PLASMA MEMBRANE 0.0426795399160035 0.0134106516810369 301 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication biological_process molecular_function integral to membrane|plasma membrane|fungal-type vacuole YCL024W KCC4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0426163607243711 0.013548741191234 302 Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication budding cell bud growth|protein phosphorylation|septin checkpoint|septin ring assembly phosphatidic acid binding|phosphatidylinositol-4,5-bisphosphate binding|phosphatidylserine binding|protein kinase activity incipient cellular bud site|cellular bud neck septin collar|plasma membrane|cellular bud neck|septin ring YEL024W RIP1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0426102757800046 0.0136460625018148 303 Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|mitochondrial respiratory chain complex III YPL270W MDL2 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0425991403307048 0.0135865965809402 304 Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis oligopeptide transport|oligopeptide export from mitochondrion oligopeptide-transporting ATPase activity mitochondrion|integral to membrane|mitochondrial inner membrane YPR097W YPR097W hom FT MITOCHONDRION 0.042573948488736 0.0136421437687794 305 Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process phosphatidylinositol binding mitochondrion YGR063C SPT4 hom FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.04239237299503 0.0140484771257748 306 Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions 7-methylguanosine mRNA capping|chromosome segregation|chromatin organization|intracellular mRNA localization|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription, DNA-dependent|regulation of transcription-coupled nucleotide-excision repair|chromatin silencing|mRNA splicing, via spliceosome|Unknown core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rDNA binding DSIF complex|nuclear telomeric heterochromatin|rDNA heterochromatin|mating-type region heterochromatin|kinetochore YCL011C GBP2 hom FT RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.0423916062541858 0.0140932330104334 307 Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication poly(A)+ mRNA export from nucleus|telomere maintenance RNA binding|telomeric DNA binding nucleus|cytoplasmic stress granule YLR294C_d YLR294C hom 0.0423534713928538 0.014136908058662 308 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Unknown Unknown Unknown YNR042W_d YNR042W hom 0.042084982031238 0.0147607370733939 309 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Unknown Unknown Unknown YIL155C GUT2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0420489126137181 0.0148463649451894 310 Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner NADH oxidation|replicative cell aging|glycerol metabolic process glycerol-3-phosphate dehydrogenase activity mitochondrion|integral to mitochondrial outer membrane YDR098C GRX3 hom FT ION HOMEOSTASIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0420102481237202 0.0150137124631882 311 Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36 cellular iron ion homeostasis|cellular response to oxidative stress|actin cytoskeleton organization disulfide oxidoreductase activity cytosol|nucleus YKL151C YKL151C hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0419670522445484 0.0150875502161447 312 NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress nicotinamide nucleotide metabolic process ATP-dependent NAD(P)H-hydrate dehydratase activity cytoplasm YDR424C DYN2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0418496957346594 0.0153272271086877 313 Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex microtubule-based process|actin filament-based process|nuclear pore complex assembly molecular_function cytoplasmic dynein complex|cytoplasmic microtubule|peroxisome|nuclear pore YKL080W VMA5 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0418417510308264 0.0153466847027171 314 Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane vacuolar acidification proton-transporting ATPase activity, rotational mechanism fungal-type vacuole membrane|vacuolar proton-transporting V-type ATPase, V1 domain|integral to membrane YOR316C-A_p YOR316C-A hom 0.0418328732929512 0.0153684531264606 315 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGR184C UBR1 hom FT PROTEOLYSIS 0.0418262922274749 0.0155538777317285 316 E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) regulation of dipeptide transport|ubiquitin-dependent protein catabolic process via the N-end rule pathway|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process|protein polyubiquitination ubiquitin-protein ligase activity proteasome regulatory particle, base subcomplex|cytoplasm YMR251W-A HOR7 hom FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.0415141071747325 0.0162159606010041 317 Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication plasma membrane organization molecular_function endoplasmic reticulum|plasma membrane YJR113C RSM7 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.041503008633095 0.016308383035663 318 Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YER143W DDI1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|PLASMA MEMBRANE 0.0414886319085564 0.0162337633653197 319 DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains vesicle-mediated transport|protein secretion|ubiquitin-dependent protein catabolic process ubiquitin binding|SNARE binding|aspartic-type endopeptidase activity plasma membrane YLR202C_d YLR202C hom 0.0414884720085291 0.0163139398971985 320 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Unknown Unknown Unknown YDR219C MFB1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.0413694177249249 0.0165432522129546 321 Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|mitochondrion organization molecular_function extrinsic to mitochondrial outer membrane|SCF ubiquitin ligase complex YOR244W ESA1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0413466047878576 0.0166030631842756 322 Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy DNA repair|histone acetylation|regulation of cell cycle|regulation of transcription by chromatin organization|DNA-dependent transcription, elongation|chromatin silencing at rDNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter H4 histone acetyltransferase activity|histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YGR288W MAL13 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0412973544935285 0.0167328352321093 323 MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C cellular carbohydrate metabolic process|regulation of transcription, DNA-dependent sequence-specific DNA binding transcription factor activity nucleus YNL072W RNH201 hom FT NUCLEUS 0.0412782649695084 0.0167833740380115 324 Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome DNA replication, removal of RNA primer ribonuclease H activity ribonuclease H2 complex|nucleus YDL001W RMD1 hom FT CELL CYCLE 0.0412448479591821 0.0168721668533115 325 Cytoplasmic protein required for sporulation biological_process molecular_function cytoplasm YBL010C_p YBL010C hom FT NUCLEUS 0.0412274311239163 0.0169186085509491 326 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles biological_process molecular_function clathrin-coated vesicle YNL220W ADE12 hom FT NUCLEOTIDE METABOLISM 0.0410995511744063 0.0172630461059002 327 Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence purine nucleotide biosynthetic process DNA replication origin binding|adenylosuccinate synthase activity cytoplasm YDR340W_d YDR340W hom 0.0410242601355914 0.0174687007243109 328 Putative protein of unknown function Unknown Unknown Unknown YMR216C SKY1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0409639903128238 0.0176348695849129 329 SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 cellular cation homeostasis|cellular ion homeostasis|response to drug|protein phosphorylation|positive regulation of protein import into nucleus|regulation of cell size|mRNA splice site selection protein serine/threonine kinase activity|protein kinase activity cytoplasm YPR019W MCM4 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0409562347540068 0.0176563525477015 330 Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|double-strand break repair via break-induced replication|DNA replication initiation|pre-replicative complex assembly four-way junction helicase activity|DNA helicase activity|DNA replication origin binding|single-stranded DNA-dependent ATPase activity|single-stranded DNA binding|ATP-dependent 3'-5' DNA helicase activity replication fork protection complex|nuclear replication fork|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YNL193W_p YNL193W hom 0.0409309308328321 0.0177266041379357 331 Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis biological_process molecular_function cellular_component YLR067C PET309 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0407991787459866 0.0180963594567421 332 Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) positive regulation of mitochondrial translational initiation|RNA metabolic process|mitochondrial respiratory chain complex IV biogenesis|aerobic respiration translation regulator activity extrinsic to mitochondrial inner membrane|mitochondrion|integral to membrane|mitochondrial inner membrane YJL136W-A_p YJL136W-A hom 0.0407976512100316 0.0183084894791938 333 Putative protein of unknown function; identified by SAGE biological_process molecular_function cellular_component YNL187W SWT21 hom FT RNA PROCESSING|NUCLEUS 0.0406534049254243 0.0185133173174562 334 Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats mRNA splicing, via spliceosome molecular_function small nuclear ribonucleoprotein complex|nucleus YPL034W_p YPL034W hom 0.040629589250239 0.0185822301580164 335 Putative protein of unknown function; YPL034W is not essential gene biological_process molecular_function cellular_component YBR212W NGR1 hom FT MITOCHONDRION ORGANIZATION 0.0406202937611757 0.0186091883201156 336 RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase 3'-UTR-mediated mRNA destabilization|mitochondrion organization mRNA binding cytoplasmic mRNA processing body|cytoplasmic stress granule|perinuclear region of cytoplasm|cytoplasm YGR148C RPL24B hom FT TRANSLATION|RIBOSOME 0.0405305967848495 0.0188710839669219 337 Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YNL274C GOR1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS|MITOCHONDRION 0.0405046380320377 0.0189474765561817 338 Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress glyoxylate catabolic process|metabolic process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor|glyoxylate reductase activity mitochondrion|nucleus|cytoplasm YBR138C_p YBR138C hom 0.0404710553635931 0.0190467060919631 339 Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm YBR163W EXO5 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0404219258085998 0.0191926914283252 340 Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 mitochondrial genome maintenance single-stranded DNA specific 5'-3' exodeoxyribonuclease activity mitochondrion YGL217C_d YGL217C hom 0.0403401787751712 0.0194377645952905 341 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Unknown Unknown Unknown YPR166C MRP2 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0403026488234218 0.0195511897296376 342 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YHR095W_d YHR095W hom 0.0403017066447547 0.0195540446557346 343 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL189C-A COA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0402574344170671 0.0196886064158253 344 Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p mitochondrial respiratory chain complex IV assembly molecular_function mitochondrial matrix YDL172C_d YDL172C hom 0.0402386343238728 0.0197459918037037 345 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YEL050C RML2 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|TRANSLATION|MITOCHONDRION|RIBOSOME 0.0401669456267002 0.0199661561672248 346 Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor cytoplasmic translation|fatty acid metabolic process structural constituent of ribosome mitochondrial large ribosomal subunit YOR057W SGT1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|SIGNALING|UBIQUITIN LIGASE COMPLEX 0.0401068057746461 0.020152501708724 347 Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress regulation of cell cycle|kinetochore assembly|cAMP-mediated signaling|protein complex assembly|protein ubiquitination protein binding, bridging|chaperone binding ubiquitin ligase complex YLR405W DUS4 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS 0.040079218864655 0.0203132106022762 348 Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p tRNA modification tRNA dihydrouridine synthase activity cellular_component YIL156W UBP7 hom FT PROTEOLYSIS 0.0400581280012911 0.020304440637919 349 Ubiquitin-specific protease that cleaves ubiquitin-protein fusions protein deubiquitination ubiquitin-specific protease activity cytoplasm YAR035W YAT1 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0400312927932424 0.0203886283072783 350 Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated carnitine metabolic process|alcohol metabolic process carnitine O-acetyltransferase activity mitochondrion YDL144C YDL144C hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0400033362960946 0.0204766572237725 351 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YOL086W-A MHF1 hom FT RESPONSE TO DNA DAMAGE 0.0398781701418168 0.0208748527932792 352 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 response to DNA damage stimulus molecular_function cellular_component YBL080C PET112 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0398518775436115 0.0209593505356001 353 Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog glutaminyl-tRNAGln biosynthesis via transamidation|mitochondrial translation glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity mitochondrion|glutamyl-tRNA(Gln) amidotransferase complex YPR088C SRP54 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS 0.0398055547566869 0.0211089452063122 354 Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|SRP-dependent cotranslational protein targeting to membrane|protein targeting to ER 7S RNA binding|GTP binding|signal sequence binding signal recognition particle, endoplasmic reticulum targeting YDR194C MSS116 hom FT RNA PROCESSING|TRANSLATION|MITOCHONDRION 0.0397624164624726 0.0212684007237873 355 DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing RNA folding|Group I intron splicing|Group II intron splicing RNA binding|RNA strand annealing activity|ATP-dependent RNA helicase activity mitochondrion|mitochondrial matrix YGR076C MRPL25 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0397141935452665 0.021406714084493 356 Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YDR095C_d YDR095C hom 0.0396983062407926 0.0215953582158961 357 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR348C PUT4 hom FT PLASMA MEMBRANE 0.0396092533497511 0.0217532457923068 358 Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells neutral amino acid transport|proline transport|gamma-aminobutyric acid transport neutral amino acid transmembrane transporter activity|L-proline transmembrane transporter activity integral to membrane|plasma membrane YOR305W RRG7 hom FT MITOCHONDRION 0.0395800003305157 0.0218507101858552 359 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene biological_process molecular_function mitochondrion YGR204C-A_p YGR204C-A hom 0.0394967914568292 0.022130022722439 360 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YMR273C ZDS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL WALL ORG/BIOGENESIS|RNA LOCALIZATION|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH|NUCLEAR PORE 0.0394830709596703 0.0221763760682787 361 Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication mRNA export from nucleus|positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|chromatin silencing|establishment of cell polarity|regulation of protein localization|cell aging protein phosphatase type 2A regulator activity incipient cellular bud site|nuclear pore|cellular bud neck|cellular bud tip|cytoplasm YAR035C-A_p YAR035C-A hom 0.0394333649346249 0.0223450104375799 362 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis biological_process molecular_function cellular_component YER095W RAD51 hom FF|FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.039413663901486 0.0224121565967961 363 Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein strand invasion|telomere maintenance via recombination|heteroduplex formation|meiotic joint molecule formation|reciprocal meiotic recombination|double-strand break repair via single-strand annealing recombinase activity|single-stranded DNA binding|DNA-dependent ATPase activity condensed nuclear chromosome|nuclear chromosome YDR097C MSH6 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0392716130975371 0.0229015125789372 364 Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p mismatch repair|meiotic mismatch repair guanine/thymine mispair binding|four-way junction DNA binding|ATPase activity|single base insertion or deletion binding|ATP binding MutSalpha complex|nucleus YER126C NSA2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0392313466972282 0.0230419045427371 365 Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleus|preribosome, large subunit precursor YLR091W GEP5 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0392263020032128 0.0230595458205734 366 Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine mitochondrial genome maintenance molecular_function mitochondrion YGR093W_p YGR093W hom FT NUCLEUS 0.0392249158872234 0.0230643951163839 367 Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus biological_process molecular_function nucleus YNL307C MCK1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION 0.0391102678960173 0.0234685654654412 368 Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family meiosis|double-strand break repair via nonhomologous end joining|ascospore formation|protein phosphorylation|response to stress|mitotic sister chromatid segregation protein serine/threonine/tyrosine kinase activity|protein serine/threonine kinase activity|protein kinase activity cellular_component YDR425W SNX41 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT 0.0390801948902958 0.0236379665944108 369 Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p retrograde transport, endosome to Golgi phosphatidylinositol binding|phosphatidylinositol-3-phosphate binding endosome YNL038W GPI15 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0390427180124404 0.023730419419898 370 Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YML083C YML083C hom 0.0390364275935959 0.0237321166269521 371 Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component YDR065W RRG1 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0389564867354783 0.0240203315711993 372 Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies mitochondrial genome maintenance|vacuolar acidification molecular_function mitochondrion YHR133C NSG1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.038953406297725 0.024031498079581 373 Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress sterol biosynthetic process unfolded protein binding integral to membrane|endoplasmic reticulum YHR068W DYS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0389419115183897 0.024327393574068 374 Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric peptidyl-lysine modification to hypusine transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm YPL172C COX10 hom FT COFACTOR METABOLISM|MITOCHONDRION 0.0388824750101744 0.0243748192246968 375 Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders heme a biosynthetic process protoheme IX farnesyltransferase activity mitochondrion|integral to membrane YLL026W HSP104 hom FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0388728880370328 0.0243249748065988 376 Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress trehalose metabolism in response to heat stress|protein folding in endoplasmic reticulum|protein unfolding|chaperone cofactor-dependent protein refolding|inheritance of oxidatively modified proteins involved in replicative cell aging|cellular heat acclimation ADP binding|unfolded protein binding|ATPase activity, coupled|chaperone binding|ATP binding TRC complex|nucleus|cytoplasm YGL191W COX13 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0388090435195388 0.0245812527400821 377 Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes mitochondrial respiratory chain supercomplex assembly|aerobic respiration enzyme regulator activity|cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YJR135W-A TIM8 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0386383799693248 0.0251974782621816 378 Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome protein import into mitochondrial inner membrane protein transporter activity mitochondrion|integral to membrane|mitochondrial intermembrane space protein transporter complex YAL044W-A_p YAL044W-A hom 0.0385064845503747 0.0257000022739481 379 Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein biological_process molecular_function cellular_component YKL026C GPX1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0384941213726497 0.0257475473420909 380 Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress cellular response to oxidative stress|peroxisome organization phospholipid-hydroperoxide glutathione peroxidase activity|glutathione peroxidase activity extrinsic to mitochondrial outer membrane|peroxisomal matrix YBR072C-A_p YBR072C-A hom 0.0384791746475078 0.0258051294747877 381 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YNL116W DMA2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0383364527519363 0.0263605935860756 382 Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains establishment of mitotic spindle orientation|protein autoubiquitination|septin ring assembly|mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity cytoplasm YPL005W AEP3 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.038308236365035 0.0265843707911191 383 Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA mRNA metabolic process|mitochondrial translational initiation molecular_function mitochondrion|mitochondrial inner membrane|cytoplasm|extrinsic to membrane YJL063C MRPL8 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0382468158417872 0.0267147090540924 384 Mitochondrial ribosomal protein of the large subunit mitochondrial genome maintenance|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YHL041W_d YHL041W hom 0.0382417036334402 0.0267350283056361 385 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YBR263W SHM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0382258325451804 0.0267981953871803 386 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine serine family amino acid biosynthetic process|one-carbon metabolic process glycine hydroxymethyltransferase activity mitochondrion YOR325W_d YOR325W hom 0.0380404489148834 0.0275456161185755 387 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Unknown Unknown Unknown YGR165W MRPS35 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0379289555667792 0.0281679237021228 388 Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YGL220W FRA2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.037924887833299 0.0287797022408753 389 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel negative regulation of transcription from RNA polymerase II promoter in response to iron molecular_function cytosol|nucleus|cytoplasm YMR144W_p YMR144W hom FT NUCLEUS 0.0378566794592495 0.0283041992345962 390 Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene biological_process molecular_function nucleus YOL138C RTC1 hom 0.0375803733083814 0.0294786041017496 391 Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif biological_process molecular_function ribosome|fungal-type vacuole|Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YLR134W PDC5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0375413596609639 0.0296477563580666 392 Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism L-phenylalanine catabolic process|glucose catabolic process to ethanol|pyruvate metabolic process|tryptophan catabolic process|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway pyruvate decarboxylase activity nucleus|cytoplasm YPL055C LGE1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.037515130973952 0.0297619450374968 393 Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division histone methylation|protein monoubiquitination|regulation of cell size|histone ubiquitination|premeiotic DNA replication molecular_function nucleus YOR189W IES4 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0374971766359065 0.0298403284311 394 Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses response to DNA damage stimulus molecular_function nucleus|Ino80 complex YER110C KAP123 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0374909837826867 0.0299898314875576 395 Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 NLS-bearing substrate import into nucleus|Unknown|protein import into nucleus|ribosomal protein import into nucleus protein transporter activity|nuclear localization sequence binding integral to membrane|nucleus|nuclear pore|cytoplasm YDL069C CBS1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0374670616558962 0.0301195222269265 396 Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial respiratory chain complex III biogenesis|positive regulation of mitochondrial translation translation regulator activity extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial ribosome YML110C COQ5 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.03741362814109 0.030207414438181 397 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes ubiquinone biosynthetic process|aerobic respiration 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity mitochondrion|mitochondrial matrix YOR034C AKR2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS 0.0374132677706265 0.0302090061569845 398 Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication endocytosis|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus YOR046C DBP5 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH|NUCLEAR PORE 0.0373055813130209 0.0306878845140711 399 Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p translational termination|mRNA export from nucleus RNA helicase activity|inositol hexakisphosphate binding|RNA-dependent ATPase activity nuclear pore cytoplasmic filaments|nucleus|polysome|nuclear pore|cellular bud tip|cytoplasm YIL096C_p YIL096C hom FT NUCLEUS 0.0372510388764398 0.030932909054353 400 Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis biological_process S-adenosylmethionine-dependent methyltransferase activity nucleolus|nucleus YLL029W FRA1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS 0.0372437847507881 0.0310408279775287 401 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation negative regulation of transcription from RNA polymerase II promoter in response to iron molecular_function cytosol|cytoplasm YGL199C_d YGL199C hom 0.0370874002809346 0.0316781311359669 402 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Unknown Unknown Unknown YCR023C YCR023C hom 0.0370624035393079 0.0318698625603736 403 Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function fungal-type vacuole membrane|integral to membrane YLR381W CTF3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0370386390848231 0.0319031479845842 404 Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 chromosome segregation|DNA replication initiation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YBL099W ATP1 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0367988892385486 0.0330556952445314 405 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrial nucleoid|proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, catalytic core YFL027C GYP8 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM 0.0367779231927417 0.0331296415148636 406 GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport vesicle-mediated transport Rab GTPase activator activity intracellular YIL160C POT1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0366912201798754 0.0335463600376109 407 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids fatty acid beta-oxidation mRNA binding|acetyl-CoA C-acyltransferase activity peroxisomal matrix|peroxisome YHR022C-A_p YHR022C-A hom 0.0366884899100624 0.0335595549234036 408 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YOR347C PYK2 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0366839804273861 0.0335813581308891 409 Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication pyruvate metabolic process pyruvate kinase activity cytosol|mitochondrion YOR161C-C_p YOR161C-C hom 0.0365904575523285 0.0340362759089372 410 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YIL101C XBP1 hom FT NUCLEUS 0.0365302863873629 0.0343317382945877 411 Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress response to stress sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YMR314W PRE5 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0364334520504806 0.0349474738471457 412 Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YMR109W MYO5 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0364110199873397 0.0349238582834853 413 One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization bipolar cellular bud site selection|endocytosis|exocytosis|fungal-type cell wall organization|response to salt stress|receptor-mediated endocytosis|actin cortical patch localization|response to osmotic stress microfilament motor activity mating projection tip|actin cortical patch YMR138W CIN4 hom FT SIGNALING 0.0363652455219923 0.0351534173240727 414 GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog protein folding|tubulin complex assembly|tubulin complex biogenesis GTP binding cytoplasm YBR029C CDS1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0362979313645831 0.0354933359417165 415 Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids phosphatidylserine metabolic process|phosphatidylglycerol biosynthetic process phosphatidate cytidylyltransferase activity mitochondrion|integral to membrane|endoplasmic reticulum YNL168C_p FMP41 hom FT MITOCHONDRION 0.0362902483600396 0.0355323107140251 416 Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YPL104W MSD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0362238122396479 0.035870856407986 417 Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene mitochondrial aspartyl-tRNA aminoacylation aspartate-tRNA ligase activity mitochondrion YPL154C PEP4 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.0362209742079126 0.0358853794999559 418 Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates microautophagy|proteolysis involved in cellular protein catabolic process|cellular response to starvation peptidase activity fungal-type vacuole lumen|mitochondrion|fungal-type vacuole YJR121W ATP2 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0361136232242357 0.0364384201081645 419 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|mitochondrial proton-transporting ATP synthase, catalytic core|integral to membrane YPL067C_p YPL067C hom 0.0361113419681722 0.0364502508057289 420 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm YFL007W BLM10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0359671613377958 0.03720464510945 421 Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 proteasome assembly|regulation of proteasomal protein catabolic process proteasome binding|peptidase activator activity proteasome core complex|proteasome complex|integral to membrane|nucleus YLL056C_p YLL056C hom 0.0359591119764566 0.0373038092655873 422 Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin biological_process molecular_function cellular_component YGL143C MRF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0359394646582214 0.0374646881594726 423 Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability translational termination|mitochondrial translational termination|mitochondrial translation translation release factor activity mitochondrion YOR372C NDD1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0359017903292167 0.0377794259520884 424 Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes positive regulation of transcription involved in G2/M-phase of mitotic cell cycle transcription coactivator activity nucleus YGR150C CCM1 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0358716657377003 0.0379119031314849 425 Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport rRNA metabolic process|mitochondrial RNA processing|mitochondrial genome maintenance rRNA binding mitochondrion YOL010W RCL1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0358498330453638 0.0378283069121454 426 Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA-3'-phosphate cyclase activity|endoribonuclease activity|enzyme activator activity nucleolus|90S preribosome YJL125C GCD14 het FT RNA PROCESSING|NUCLEUS 0.035835305277955 0.0379061446364129 427 Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression tRNA methylation tRNA (adenine-N1-)-methyltransferase activity tRNA (m1A) methyltransferase complex|nucleus YKL018C-A_p YKL018C-A hom 0.0357482898912305 0.0383752165871051 428 Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YOR343C_d YOR343C hom 0.0357306967706983 0.0384706524479829 429 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL119C-A_p YPL119C-A hom 0.0357173706590677 0.0385430755836153 430 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YBR069C TAT1 hom FF|FT PLASMA MEMBRANE 0.0356789268147655 0.0391909661275774 431 Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress amino acid transport|tyrosine transport|tryptophan transport|transmembrane transport amino acid transmembrane transporter activity|low-affinity tryptophan transmembrane transporter activity integral to membrane|plasma membrane YGR208W SER2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0356763349421536 0.0388832250531966 432 Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source L-serine biosynthetic process phosphoserine phosphatase activity nucleus|cytoplasm YCR087W_d YCR087W hom 0.0356711560271015 0.03879513522699 433 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Unknown Unknown Unknown YIL079C AIR1 hom FT RNA PROCESSING|NUCLEUS 0.0356520518587747 0.0388997396478124 434 Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent snRNA catabolic process polynucleotide adenylyltransferase activity nucleolus|TRAMP complex YOL099C_d YOL099C hom 0.0355401580933835 0.0395172322178916 435 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Unknown Unknown Unknown YPL096W PNG1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|MITOCHONDRION 0.035472387784406 0.0398952556993327 436 Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p misfolded or incompletely synthesized protein catabolic process|protein deglycosylation peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity cytosol|mitochondrion|nucleus YOL124C TRM11 hom FT RNA PROCESSING 0.0354269079308695 0.0401506587535001 437 Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain tRNA methylation S-adenosylmethionine-dependent methyltransferase activity|RNA binding|tRNA (guanine-N2-)-methyltransferase activity cytoplasm|tRNA (m2G10) methyltransferase complex YPR149W NCE102 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE 0.0354202723344604 0.0401880381708984 438 Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) eisosome assembly|protein secretion|negative regulation of protein phosphorylation|plasma membrane organization|establishment of protein localization to plasma membrane molecular_function mitochondrion|integral to membrane|membrane raft|endoplasmic reticulum|cellular bud membrane|plasma membrane|mating projection membrane|cytoplasm YGR065C VHT1 het FT PLASMA MEMBRANE 0.0353055602915036 0.0409593040423972 439 High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin biotin transport biotin transporter activity integral to membrane|plasma membrane YDR301W CFT1 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0352711493794183 0.0410358862970167 440 RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF mRNA cleavage|termination of RNA polymerase II transcription|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mitochondrion|mRNA cleavage and polyadenylation specificity factor complex YER137C_p YER137C hom 0.0352636337281561 0.0410790149943794 441 Putative protein of unknown function biological_process molecular_function cellular_component YMR235C RNA1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|GENE SILENCING|NUCLEUS 0.0351933221631414 0.0414843617091563 442 GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport rRNA metabolic process|ribosomal subunit export from nucleus|tRNA metabolic process|rRNA export from nucleus|RNA import into nucleus|protein import into nucleus|chromatin silencing at telomere Ran GTPase activator activity cytosol|integral to membrane|nucleus YPR024W YME1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0351662369968504 0.041763321060522 443 Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover chronological cell aging|misfolded or incompletely synthesized protein catabolic process|mitochondrion organization ATP-dependent peptidase activity mitochondrion|mitochondrial inner membrane|i-AAA complex YMR287C DSS1 hom FT MITOCHONDRION 0.0349993499909651 0.0430861839814315 444 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs mitochondrial RNA catabolic process exoribonuclease II activity mitochondrion|mitochondrial degradosome YGR191W HIP1 het FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.03497734146586 0.0427507241147542 445 High-affinity histidine permease, also involved in the transport of manganese ions manganese ion transport|histidine transport high affinity L-histidine transmembrane transporter activity integral to membrane|plasma membrane YDR322W MRPL35 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0348931268965556 0.0441994193145523 446 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOR007C SGT2 hom FT PROTEIN LOCALIZATION 0.0348108888139786 0.0437488422700241 447 Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress response to heat|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YNR035C ARC35 het FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0348085713885896 0.0439202441429298 448 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin mitochondrion inheritance|receptor-mediated endocytosis|microtubule cytoskeleton organization|actin cytoskeleton organization structural molecule activity cytosol|Arp2/3 protein complex YOR146W_d YOR146W het 0.0347973302051521 0.0439254475127471 449 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Unknown Unknown Unknown YMR023C MSS1 hom FT RNA PROCESSING|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0346561679463191 0.0446941818587986 450 Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 mitochondrial tRNA wobble uridine modification|Unknown GTP binding mitochondrion|mitochondrial inner membrane YHL005C_d YHL005C hom 0.0346300299832009 0.0448874947130396 451 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Unknown Unknown Unknown YPR189W SKI3 hom FT NUCLEUS 0.0345951666477781 0.0450716029893425 452 Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function Ski complex YLR150W STM1 hom FT SIGNALING|TRANSLATION|NUCLEUS|RIBOSOME 0.0344972258861194 0.045683189257339 453 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery translational elongation|TOR signaling cascade|regulation of translational initiation in response to stress|telomere maintenance|anti-apoptosis triplex DNA binding|DNA binding|telomeric DNA binding cytosolic ribosome|polysome|cytoplasm YIR012W SQT1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RIBOSOME 0.0343946576585851 0.0463311486872692 454 Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress ribosomal large subunit assembly molecular_function cytosol|cytosolic ribosome|cytoplasm YOR211C MGM1 hom FT MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0343397753799561 0.0466810242139811 455 Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy mitochondrion organization|mitochondrial genome maintenance|mitochondrial fusion GTPase activity intrinsic to mitochondrial inner membrane|extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial inner boundary membrane|mitochondrial crista|mitochondrial intermembrane space YER051W JHD1 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0343300968758 0.0467429548145549 456 JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe histone demethylation methylated histone residue binding|histone demethylase activity (H3-K36 specific) cellular_component YPL083C SEN54 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0343111893524588 0.0468641392512815 457 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YIL115C NUP159 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0342999854791211 0.0469360730993425 458 FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|protein export from nucleus|ribosomal large subunit export from nucleus|ncRNA export from nucleus|nuclear pore distribution|ribosomal small subunit export from nucleus nucleocytoplasmic transporter activity|adenyl-nucleotide exchange factor activity nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YGL202W ARO8 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0342809407306976 0.0470585619118739 459 Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis aromatic amino acid family metabolic process aromatic-amino-acid:2-oxoglutarate aminotransferase activity|2-aminoadipate transaminase activity cytoplasm YGR190C_d YGR190C het 0.0342488730894368 0.0472654164587169 460 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Unknown Unknown Unknown YBR123C TFC1 het FT NUCLEUS 0.0341971042548096 0.0476009676985352 461 One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YKL072W STB6 hom 0.0341544341476368 0.0478790463549708 462 Protein that binds Sin3p in a two-hybrid assay biological_process molecular_function cellular_component YBR028C YPK3 hom FT PROTEIN PHOSPHORYLATION 0.0341511712587495 0.0480002492932773 463 An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner biological_process protein kinase activity cytoplasm YNL068C FKH2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|MITOCHONDRION ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0341120175935888 0.0481568238459057 464 Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication negative regulation of chromatin silencing at silent mating-type cassette|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of transcription involved in G2/M-phase of mitotic cell cycle|negative regulation of transcription involved in G1 phase of mitotic cell cycle|Unknown|chromatin remodeling|negative regulation of transcription involved in G2/M-phase of mitotic cell cycle RNA polymerase II transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity nucleus YPL079W RPL21B hom FT TRANSLATION|RIBOSOME 0.0341035916755641 0.0482121643059181 465 Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YFR045W_p YFR045W hom FT MITOCHONDRION 0.0340305687203963 0.0486940112201928 466 Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white transport transporter activity integral to membrane|mitochondrial inner membrane YOR100C CRC1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.034002793071181 0.0488783486859752 467 Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation fatty acid metabolic process carnitine:acyl carnitine antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YER093C-A AIM11 hom 0.033992570724563 0.0489463381372161 468 Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication biological_process molecular_function cellular_component YDR031W MIC14 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.0339568679763935 0.0491844221221192 469 Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress aerobic respiration molecular_function nucleus|mitochondrial intermembrane space|cytoplasm YGR215W RSM27 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0339414084738191 0.0492878148679674 470 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YOL127W RPL25 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0339334140706499 0.0493413526903845 471 Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YMR084W_p YMR084W hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.033907118869252 0.0495177935073779 472 Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component YIL093C RSM25 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0338907932301747 0.0496276045098502 473 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YKR025W RPC37 het FT NUCLEUS 0.033868021666677 0.0498151914702713 474 RNA polymerase III subunit C37 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YDR243C PRP28 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0338626562669792 0.0499538620782562 475 RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site cis assembly of pre-catalytic spliceosome ATP-dependent RNA helicase activity spliceosomal complex|U5 snRNP YLR346C_p YLR346C hom FT MITOCHONDRION 0.0338258068917777 0.050100946389516 476 Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene biological_process molecular_function mitochondrion YOR150W MRPL23 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0337943008995902 0.0502808171848501 477 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YGR273C_p YGR273C hom 0.0337633995532178 0.0504915229989192 478 Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene biological_process molecular_function cellular_component YJL207C LAA1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0336868759677654 0.0512939834662198 479 AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene protein localization|retrograde transport, endosome to Golgi molecular_function AP-1 adaptor complex|ribosome|clathrin-coated vesicle YOL013W-B_d YOL013W-B hom 0.0336855658310318 0.0510255240826487 480 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Unknown Unknown Unknown YMR256C COX7 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0335827949304071 0.0519476201283672 481 Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YKR070W_p YKR070W hom FT MITOCHONDRION 0.0335357512732647 0.0521718635520287 482 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YIR008C PRI1 het FT NUCLEUS|CHROMOSOME 0.0335299892264214 0.0521070772118494 483 Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA replication initiation|DNA replication single-stranded DNA binding|DNA primase activity alpha DNA polymerase:primase complex|nuclear replication fork YMR286W MRPL33 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0334886325782901 0.0526091986390727 484 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YIL052C RPL34B hom FF|FT TRANSLATION|RIBOSOME 0.0334526642726218 0.0526517228128904 485 Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJR034W PET191 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0334483785573287 0.0526820482547284 486 Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|integral to mitochondrial inner membrane YER087C-B SBH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0333359642538823 0.0534827109389888 487 Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation ARF guanyl-nucleotide exchange factor activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex YJL034W KAR2 het FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOPLASMIC RETICULUM 0.0333316177557471 0.0535138714098161 488 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p karyogamy involved in conjugation with cellular fusion|posttranslational protein targeting to membrane, translocation|ER-associated protein catabolic process|SRP-dependent cotranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|ATPase activity endoplasmic reticulum|luminal surveillance complex YPL117C IDI1 het FT LIPID METABOLISM|NUCLEUS 0.0331728668521004 0.0546623995097724 489 Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability farnesyl diphosphate biosynthetic process isopentenyl-diphosphate delta-isomerase activity nucleus|cytoplasm YCL021W-A_p YCL021W-A hom 0.0331383599507488 0.0550234098572748 490 Putative protein of unknown function biological_process molecular_function cellular_component YBL027W RPL19B hom FT TRANSLATION|RIBOSOME 0.0330722865739379 0.0555098982973471 491 Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJR051W OSM1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0329188229062845 0.056579905993251 492 Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity mitochondrion YPL237W SUI3 het FT TRANSLATION|RIBOSOME 0.0328757461525613 0.0568672361474842 493 Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding translational initiation translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YFL018C LPD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0328134207887035 0.0573389957919921 494 Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes L-serine biosynthetic process|2-oxoglutarate metabolic process|leucine catabolic process|glycine catabolic process|isoleucine catabolic process|pyruvate metabolic process|valine catabolic process|hydrogen peroxide metabolic process glycine dehydrogenase (decarboxylating) activity|pyruvate dehydrogenase activity|oxoglutarate dehydrogenase (succinyl-transferring) activity|dihydrolipoyl dehydrogenase activity mitochondrial nucleoid|mitochondrion|glycine cleavage complex|mitochondrial oxoglutarate dehydrogenase complex YHR126C_p ANS1 hom FT PLASMA MEMBRANE 0.0328107697077015 0.0573591345477121 495 Putative GPI protein; transcription dependent upon Azf1p biological_process molecular_function cellular_component YKL077W_p YKL077W hom 0.0327749321998481 0.0576319491363195 496 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YLR236C_d YLR236C hom 0.0327338033561388 0.057946372183795 497 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML099C ARG81 hom FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0326510770532339 0.058771542362873 498 Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p arginine metabolic process|negative regulation of calcium ion-dependent exocytosis transcription cofactor activity nucleus YPR191W QCR2 hom FT OXIDATION-REDUCTION PROCESS|PROTEOLYSIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0326347559334322 0.0587094244593301 499 Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial crista|mitochondrial respiratory chain complex III YDR005C MAF1 hom FT NUCLEUS 0.03260374933389 0.0589500036671371 500 Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions negative regulation of transcription from RNA polymerase III promoter RNA polymerase III core binding nucleolus|nucleus|cytoplasm YDR070C FMP16 hom FT MITOCHONDRION 0.032559690638814 0.0592932602413533 501 Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion YMR032W HOF1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0325539050305512 0.0593384582027804 502 Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p cytokinesis after mitosis|barrier septum assembly involved in cell cycle cytokinesis|response to DNA damage stimulus|cytokinesis cytoskeletal protein binding cellular bud neck contractile ring|cellular bud neck septin ring YJL195C_d YJL195C het FF 0.0325068377958379 0.0597072167841783 503 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Unknown Unknown Unknown YOL095C HMI1 hom FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0324691964047793 0.0600034921540122 504 Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD mitochondrial genome maintenance|mitochondrial DNA metabolic process ATP-dependent 3'-5' DNA helicase activity mitochondrion|mitochondrial matrix YNL267W PIK1 het FF|FT LIPID METABOLISM|SIGNALING|NUCLEUS|GOLGI APPARATUS 0.0324258482209617 0.0607689713609594 505 Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity trans-Golgi network|nucleus YGR077C PEX8 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0324148546827365 0.0604333644152708 506 Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p protein targeting to peroxisome|peroxisome organization peroxisome targeting sequence binding peroxisomal matrix|peroxisomal membrane|extrinsic to intraperoxisomal membrane YKR094C RPL40B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION|NUCLEUS|MITOCHONDRION|RIBOSOME 0.0324037415235355 0.0607137015058712 507 Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication cytoplasmic translation|protein ubiquitination|ribosome biogenesis structural constituent of ribosome|protein tag mitochondrion|cytosolic large ribosomal subunit YBR025C OLA1 hom 0.0323885690760277 0.0606422115020599 508 P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress biological_process ATPase activity cytoplasm YNR032C-A HUB1 hom FT RNA PROCESSING 0.0323701025635166 0.0607892907654272 509 Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear cellular protein modification process|cell morphogenesis involved in conjugation with cellular fusion|mRNA splicing, via spliceosome protein tag mating projection YDR296W MHR1 hom FT MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0323582578506656 0.0608837851563966 510 Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress cellular response to oxidative stress|positive regulation of mitochondrial DNA replication|regulation of transcription, DNA-dependent|mitochondrial genome maintenance|DNA recombination single-stranded DNA binding|recombinase activity|DNA binding mitochondrion|nucleus YLR019W PSR2 hom FT PLASMA MEMBRANE 0.0323284885903051 0.0613925305428481 511 Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p response to stress phosphoprotein phosphatase activity plasma membrane YMR037C MSN2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0323127430737399 0.0612480239919748 512 Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|cellular response to methylmercury|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YER017C AFG3 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.032277469370967 0.061531546155579 513 Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging proteolysis|signal peptide processing|protein complex assembly|protein import into mitochondrial intermembrane space metallopeptidase activity|protein binding|ATPase activity mitochondrion|mitochondrial inner boundary membrane|mitochondrial inner membrane|m-AAA complex YPR025C CCL1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|NUCLEUS|MITOCHONDRION|RNA POL II, HOLOENZYME 0.0322736280167364 0.0615624875789371 514 Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|cyclin-dependent protein kinase regulator activity mitochondrion|holo TFIIH complex|TFIIK complex YBR227C MCX1 hom FT MITOCHONDRION 0.0322568435490588 0.0616978345452454 515 Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein refolding molecular_function mitochondrion|mitochondrial matrix YIL161W_p YIL161W hom 0.0321889402173829 0.0623650307434013 516 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene biological_process molecular_function cytoplasm YDL056W MBP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0321818767204368 0.0623053626839799 517 Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes positive regulation of transcription involved in G1/S phase of mitotic cell cycle sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription MBF transcription complex|nucleus YHR105W YPT35 hom 0.0321534601018827 0.0625369389367228 518 Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport biological_process phosphatidylinositol-3-phosphate binding endosome YLR274W MCM5 het FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0321305828761379 0.0627238894679432 519 Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase S phase of mitotic cell cycle|establishment of chromatin silencing|DNA replication initiation|regulation of DNA-dependent DNA replication initiation|negative regulation of chromatin silencing at telomere|pre-replicative complex assembly|double-strand break repair via break-induced replication|chromatin silencing at telomere DNA helicase activity|chromatin binding|DNA replication origin binding|single-stranded DNA binding replication fork protection complex|nuclear chromosome, telomeric region|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YDL231C BRE4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0320683020356856 0.0632351842479146 520 Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport endocytosis molecular_function integral to membrane YAL038W CDC19 het FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0320316240904485 0.0638148603232786 521 Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication pyruvate metabolic process|glycolysis pyruvate kinase activity cytosol|plasma membrane YBR215W HPC2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0320276034326705 0.0635711559267751 522 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding HIR complex|nucleus YML029W USA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0320128778166745 0.0637721937906183 523 Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p ER-associated protein catabolic process|mRNA splicing, via spliceosome|positive regulation of protein oligomerization protein complex scaffold Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YIL026C IRR1 het FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0320102920404438 0.0639125291970634 524 Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability spore germination|fungal-type cell wall organization|cytogamy|mitotic sister chromatid cohesion chromatin binding nucleus|nuclear mitotic cohesin complex YLR314C CDC3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0319604967356949 0.0642873115754704 525 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells septin ring disassembly|maintenance of cell polarity|cell cycle cytokinesis|septin ring assembly structural molecule activity|GTP binding|1-phosphatidylinositol binding mating projection base|septin filament array|cellular bud neck septin ring|septin complex|prospore membrane|ascospore wall YHL037C_d YHL037C hom 0.0319215678970081 0.0644534197431788 526 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNR018W RCF2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.031832372452307 0.065203366845288 527 Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|mitochondrial respiratory chain complex IV|integral to mitochondrial inner membrane YHR207C SET5 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.0318117454290637 0.0653778172425566 528 Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus histone lysine methylation S-adenosylmethionine-dependent methyltransferase activity nuclear chromatin|nucleus|cytoplasm YKL152C GPM1 het FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0318045524873659 0.0654387410145614 529 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis gluconeogenesis|glycolysis phosphoglycerate mutase activity cytosol|mitochondrion YMR027W_p YMR027W hom FT NUCLEUS 0.0317868105290854 0.0655892143718365 530 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene biological_process molecular_function nucleus|cytoplasm YMR054W STV1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0317294242484204 0.0660778734993881 531 Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|late endosome|integral to membrane|Golgi apparatus YDR006C SOK1 hom FT SIGNALING|NUCLEUS 0.0317264059567859 0.0661036578215809 532 Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 cAMP-mediated signaling molecular_function nucleus YPL152W-A_p YPL152W-A hom 0.031697194793748 0.0663536280909695 533 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YMR238W DFG5 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.0316774511884212 0.0665230215329393 534 Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p pseudohyphal growth|fungal-type cell wall biogenesis|budding cell bud growth mannosidase activity anchored to plasma membrane YPL046C ELC1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0316447177750356 0.0668859873527404 535 Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair global genome nucleotide-excision repair|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YHR051W COX6 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0316137875263714 0.0670716605408988 536 Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YIL125W KGD1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0314861294352204 0.0681830078785453 537 Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA tricarboxylic acid cycle|2-oxoglutarate metabolic process oxoglutarate dehydrogenase (succinyl-transferring) activity mitochondrial nucleoid|mitochondrion|mitochondrial matrix|mitochondrial oxoglutarate dehydrogenase complex YPL140C MKK2 hom FT SIGNALING|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION 0.0314268758291614 0.0687039682270217 538 MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication signal transduction|peroxisome degradation|protein phosphorylation MAP kinase kinase activity intracellular YKL191W DPH2 hom 0.0313906665483325 0.0690239282640088 539 Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p peptidyl-diphthamide biosynthetic process from peptidyl-histidine molecular_function cytoplasm YBL091C-A SCS22 hom FT LIPID METABOLISM 0.0313783601765572 0.0691329506105886 540 Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication phospholipid biosynthetic process|chromatin silencing at telomere molecular_function cellular_component YML035C AMD1 hom FT NUCLEOTIDE METABOLISM 0.0313541473653893 0.0697644651110588 541 AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools guanine salvage|purine nucleotide metabolic process AMP deaminase activity cytoplasm YGR216C GPI1 het FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.0313472463434041 0.06940921929243 542 Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YMR197C VTI1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0312988236588081 0.0698409803874582 543 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Golgi to vacuole transport|vacuole fusion, non-autophagic|intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to Golgi membrane YKL162C-A_d YKL162C-A hom 0.0312105596977139 0.0706336547690495 544 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR393W ATP10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0311853177406387 0.0708616972884 545 Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 mitochondrial proton-transporting ATP synthase complex assembly unfolded protein binding integral to mitochondrial membrane|mitochondrion|mitochondrial inner membrane YGR043C NQM1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0311397256400146 0.0712751175687099 546 Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift biological_process sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glyceronetransferase activity nucleus YNL314W DAL82 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0311120661575539 0.071526890020366 547 Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain allantoin catabolic process|nitrogen catabolite activation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YCR102W-A_d YCR102W-A hom 0.0310926720842112 0.0717038600840747 548 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL184C_p YNL184C hom 0.0310699697607187 0.0719114731624453 549 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YBR246W RRT2 hom FT VESICLE-MEDIATED TRANSPORT 0.0310564910994268 0.0720774166781137 550 Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-diphthamide biosynthetic process from peptidyl-histidine|endocytic recycling molecular_function endosome|cytoplasm YGL184C STR3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0309387234470175 0.0731213903697423 551 Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p methionine biosynthetic process|transsulfuration cystathionine beta-lyase activity peroxisome YDR489W SLD5 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0309375292163199 0.0731324755549533 552 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YHL011C PRS3 hom FF|FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0308818486847663 0.0736508456267306 553 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process|regulation of cell size ribose phosphate diphosphokinase activity cytoplasm YJR025C BNA1 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0308742997462816 0.0739797936897365 554 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p de novo NAD biosynthetic process from tryptophan 3-hydroxyanthranilate 3,4-dioxygenase activity cytoplasm YOR181W LAS17 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0308592475544999 0.0739051627894126 555 Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) bipolar cellular bud site selection|endocytosis|actin polymerization or depolymerization|actin filament organization|actin cortical patch localization|positive regulation of actin filament bundle assembly|response to osmotic stress|cytokinesis cytoskeletal protein binding mating projection tip|cytoplasm|actin cortical patch YNL001W DOM34 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION 0.0308221922100391 0.0743391362065765 556 Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication nuclear-transcribed mRNA catabolic process, no-go decay|ribosome disassembly|nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay|nonfunctional rRNA decay ribosome binding|endoribonuclease activity cytoplasm YMR043W MCM1 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|NUCLEUS|CHROMOSOME 0.0307867359006613 0.0745432628466381 557 Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes regulation of mating type switching|positive regulation of transcription involved in G2/M-phase of mitotic cell cycle|DNA replication initiation|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of mating-type specific transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA replication origin binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II repressing transcription factor binding nuclear chromatin YOR140W SFL1 hom FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING|NUCLEUS 0.0307733873572462 0.0747125272437996 558 Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p gene silencing|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus YOL087C DUF1 hom 0.0307259975045891 0.075117760696217 559 Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid biological_process ubiquitin binding cytoplasm YGR161W-C_p YGR161W-C hom 0.0307197693693309 0.0751768736164661 560 Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function cellular_component YPR078C_p YPR078C hom 0.0306899729889714 0.0756346499608359 561 Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible biological_process molecular_function cellular_component YIL154C IMP2' hom FF|FT RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS 0.0306625550905601 0.0761594562471927 562 Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat DNA repair|cellular carbohydrate metabolic process transcription coactivator activity cytoplasm YKL133C_p YKL133C hom 0.0306550667334975 0.0757932325073922 563 Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) biological_process molecular_function cellular_component YGL033W HOP2 hom FF|FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0305552190702175 0.0767524660926199 564 Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair synapsis|reciprocal meiotic recombination protein binding|double-stranded DNA binding condensed nuclear chromosome YKL066W_d YKL066W hom 0.0305187424004514 0.0771053540625042 565 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1 Unknown Unknown Unknown YGR064W_d YGR064W hom FF 0.0303839782296042 0.0784205869885959 566 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Unknown Unknown Unknown YER177W BMH1 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0303253284533205 0.0789986522157131 567 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|pseudohyphal growth|signal transduction involved in filamentous growth|ascospore formation|fungal-type cell wall chitin biosynthetic process|Ras protein signal transduction|DNA damage checkpoint|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle DNA replication origin binding|phosphoserine binding plasma membrane|nucleus YPL072W UBP16 hom FT PROTEOLYSIS|MITOCHONDRION 0.0303093637983768 0.0791566018732986 568 Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria protein deubiquitination ubiquitin-specific protease activity mitochondrial outer membrane|cytoplasm YDR413C_d YDR413C het 0.0302937311212634 0.0794011184252366 569 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Unknown Unknown Unknown YDR381W YRA1 het FT RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|NUCLEUS 0.0302405906988699 0.0798399604638544 570 RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member mRNA export from nucleus|transcription-coupled nucleotide-excision repair RNA binding transcription export complex YCR016W_p YCR016W hom FT NUCLEUS 0.0302290585404888 0.0799550167023919 571 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis biological_process molecular_function nucleolus|nucleus YKL136W_d YKL136W hom 0.0301842956158318 0.0804028926259637 572 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Unknown Unknown Unknown YDL067C COX9 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0301661655205973 0.0805848721368631 573 Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YGL131C SNT2 hom FT NUCLEUS|CHROMOSOME 0.0301592588345403 0.080654285387047 574 DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress biological_process DNA binding nucleus|Snt2C complex|cytoplasm YCL031C RRP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0301457038024358 0.0807906566293358 575 Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress ribosomal small subunit assembly|rRNA processing molecular_function nucleolus|nucleus|UTP-C complex|CURI complex YMR205C PFK2 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0301446550911093 0.0809370355032374 576 Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes proton transport|glycolysis mRNA binding|6-phosphofructokinase activity mitochondrion|6-phosphofructokinase complex|cytoplasm YNR005C_d YNR005C hom 0.0301226256747832 0.0810232660753656 577 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR097C MTG1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0300464279744064 0.082114554692465 578 Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals mitochondrial translation GTPase activity mitochondrion|mitochondrial inner membrane YOR158W PET123 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0300198416775546 0.0820658566452205 579 Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YGL188C-A_p YGL188C-A hom 0.0298682745613433 0.0836230957047481 580 Putative protein of unknown function biological_process molecular_function cellular_component YBL056W PTC3 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0298263798998855 0.0840577259768528 581 Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of cyclin-dependent protein kinase activity|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YOL155C HPF1 hom FT CELL WALL ORG/BIOGENESIS 0.0298209018137695 0.0841146924882167 582 Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines fungal-type cell wall organization glucosidase activity fungal-type cell wall|extracellular region YOR288C MPD1 hom FT ENDOPLASMIC RETICULUM 0.0298189341914921 0.0841351613767819 583 Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation protein folding protein disulfide oxidoreductase activity|protein disulfide isomerase activity fungal-type vacuole YBR125C PTC4 hom 0.029811210470147 0.0842155490868505 584 Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity protein dephosphorylation protein serine/threonine phosphatase activity cytoplasm YNL312W RFA2 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0298063645394826 0.0842660167562043 585 Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YNR020C ATP23 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0297522389114116 0.0848313693229114 586 Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p protein processing|mitochondrial proton-transporting ATP synthase complex assembly metalloendopeptidase activity mitochondrial inner membrane|mitochondrial intermembrane space YMR013C SEC59 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0297111907011814 0.0852621672765547 587 Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation protein glycosylation dolichol kinase activity integral to membrane|endoplasmic reticulum membrane YER030W CHZ1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0295900260222783 0.0865441014898825 588 Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress chromatin remodeling histone binding chromatin assembly complex|nucleus YLR227C ADY4 hom FT CELL CYCLE|CELL DIVISION|NUCLEUS|CYTOSKELETON 0.0295779470244487 0.086719736207034 589 Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane sporulation resulting in formation of a cellular spore structural molecule activity spindle pole body YMR070W MOT3 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0295346846322596 0.0871347721688153 590 Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion cellular hyperosmotic response|negative regulation of transcription from RNA polymerase II promoter|negative regulation of ergosterol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding nucleus YLR011W LOT6 hom FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0295284488639222 0.0872015312634783 591 FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions cellular response to oxidative stress|cytoplasmic sequestering of transcription factor NAD(P)H dehydrogenase (quinone) activity|transcription factor binding cytosol|nucleus|cytoplasm YCR031C RPS14A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.0295159417905725 0.0873355542675675 592 Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome|mRNA binding small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YPL093W NOG1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0294033962406363 0.0885490552134278 593 Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|rRNA processing GTP binding nucleolus|preribosome, large subunit precursor YPL192C PRM3 hom FT MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON 0.0293758579041962 0.0888480412897961 594 Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body karyogamy involved in conjugation with cellular fusion|nuclear membrane fusion involved in karyogamy molecular_function nuclear envelope|spindle pole body|extrinsic to nuclear outer membrane YPL156C PRM4 hom 0.0292921051162954 0.0897623519725921 595 Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function integral to membrane YKL106W AAT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0292915898876356 0.0897679999537373 596 Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis aspartate catabolic process|chronological cell aging|replicative cell aging|asparagine biosynthetic process from oxaloacetate|aspartate biosynthetic process L-aspartate:2-oxoglutarate aminotransferase activity mitochondrion YER092W IES5 hom FT NUCLEUS 0.0292781922110307 0.089914966873214 597 Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions biological_process molecular_function nucleus YPR116W RRG8 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0292260895325158 0.0906326474414572 598 Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport mitochondrial genome maintenance molecular_function mitochondrion YPL173W MRPL40 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0291498482241951 0.091332677957148 599 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YML084W_d YML084W hom 0.0291428929291795 0.0914100175820386 600 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YAR030C_d YAR030C hom 0.0291417083474778 0.0914231948185901 601 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Unknown Unknown Unknown YPL258C THI21 hom 0.0291259569608374 0.0915985576873029 602 Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p thiamine biosynthetic process phosphomethylpyrimidine kinase activity|hydroxymethylpyrimidine kinase activity cellular_component YOR300W_d YOR300W hom 0.0290645786346386 0.0922844725116834 603 Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Unknown Unknown Unknown YDR024W_d FYV1 hom 0.0290625905272649 0.0925012133938682 604 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Unknown Unknown Unknown YPL007C TFC8 het FT NUCLEUS 0.0290298371312029 0.0926745394265156 605 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter|transcription from RNA polymerase III promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YDL217C TIM22 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0290290253693145 0.0926836694339395 606 Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YDL061C RPS29B hom FT TRANSLATION|RIBOSOME 0.0290130396269076 0.0928636105826799 607 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YDL136W RPL35B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0289783180510244 0.0934019884977869 608 Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YKR072C SIS2 hom FF|FT CELL CYCLE|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0289403952730263 0.0938808072522678 609 Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis regulation of mitotic cell cycle|response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex|nucleus|cytoplasm YML054C-A_p YML054C-A hom 0.0289335271367942 0.0937627996524931 610 Putative protein of unknown function biological_process molecular_function cellular_component YDR156W RPA14 hom FT NUCLEUS 0.0289249404239055 0.0938603210659743 611 RNA polymerase I subunit A14 transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YGL063W PUS2 hom FT RNA PROCESSING|MITOCHONDRION 0.0289042146346934 0.094096043953971 612 Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion YBR179C FZO1 hom FT MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0289009949179215 0.0942799899155743 613 Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrial fusion GTPase activity|protein homodimerization activity mitochondrial outer membrane|mitochondrion|mitochondrial inner membrane|integral to mitochondrial outer membrane YGL176C_p YGL176C hom 0.0288705020726089 0.0946280482313575 614 Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype biological_process molecular_function cellular_component YPL145C KES1 hom FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0288679855152391 0.0945092319072853 615 One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication post-Golgi vesicle-mediated transport|positive regulation of phosphatase activity|maintenance of cell polarity|endocytosis|exocytosis|sterol transport phosphatidic acid binding|lipid binding|phosphatidylinositol-4,5-bisphosphate binding|oxysterol binding|sterol transporter activity|phosphatidylinositol-4-phosphate binding Golgi membrane|extrinsic to membrane|cytoplasm YOR056C NOB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|ENDOPLASMIC RETICULUM 0.0288534915602836 0.0946749399397351 616 Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress proteasome assembly|ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA endoribonuclease activity|SSU rRNA binding preribosome, small subunit precursor|nucleus|cytoplasm YBR145W ADH5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|NUCLEUS 0.0288481862118939 0.09473565364566 617 Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication NADH oxidation|ethanol biosynthetic process involved in glucose fermentation to ethanol|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity nucleus|cytoplasm YMR253C_p YMR253C hom 0.0288139118312274 0.0951286369959876 618 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene biological_process molecular_function integral to membrane|cytoplasm YJL087C TRL1 het FT RNA PROCESSING|SIGNALING|TRANSLATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.028759863499936 0.0957509954963356 619 tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|tRNA splicing, via endonucleolytic cleavage and ligation|positive regulation of translation in response to stress GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity|RNA ligase (ATP) activity|2',3'-cyclic-nucleotide 3'-phosphodiesterase activity nuclear inner membrane|polysome YDR226W ADK1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0287422059496408 0.0959550242097342 620 Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress ADP biosynthetic process|DNA replication initiation|nucleotide metabolic process adenylate kinase activity mitochondrion|mitochondrial intermembrane space|cytoplasm YMR245W_d YMR245W hom 0.0287046362527078 0.0963902902672045 621 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER085C_p YER085C hom 0.0285106171889447 0.0987135594232304 622 Putative protein of unknown function biological_process molecular_function cellular_component YLR239C LIP2 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0284615556341882 0.0992448004731947 623 Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups protein lipoylation ligase activity mitochondrion YHL025W SNF6 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|NUCLEUS|RSC COMPLEX 0.0284552997993618 0.0993191435705421 624 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|nucleotide-excision repair|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YGR094W VAS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION 0.0283937386677444 0.100053095199081 625 Mitochondrial and cytoplasmic valyl-tRNA synthetase valyl-tRNA aminoacylation valine-tRNA ligase activity mitochondrion|cytoplasm YNL336W COS1 hom 0.0283830155510803 0.100181380613041 626 Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function integral to membrane|fungal-type vacuole YOR098C NUP1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.028333583940749 0.100774448737531 627 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|nuclear pore nuclear basket|integral to membrane|nuclear pore YJL144W YJL144W hom 0.0282948407113229 0.101241232392972 628 Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress cellular response to water deprivation molecular_function cytoplasm YCR065W HCM1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0282787722706339 0.101486294852673 629 Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role positive regulation of transcription involved in S-phase of mitotic cell cycle|cellular response to oxidative stress|mitochondrion organization|spindle pole body organization|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nuclear chromatin|nucleus|cytoplasm YBL094C_d YBL094C hom 0.0282584386498388 0.101885644643011 630 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Unknown Unknown Unknown YBL055C YBL055C hom FT RESPONSE TO OXIDATIVE STRESS 0.0282574792146413 0.101692997560051 631 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases cellular response to oxidative stress|apoptotic DNA fragmentation 3'-5'-exodeoxyribonuclease activity|endonuclease activity cytoplasm YMR158C-A_p YMR158C-A hom 0.0282452391042844 0.101841350080916 632 Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene biological_process molecular_function cellular_component YGL134W PCL10 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0282411015582742 0.101891536871334 633 Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate negative regulation of glycogen biosynthetic process|regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YGL192W IME4 hom FT CELL CYCLE|NUCLEUS 0.0281757369235462 0.102840908902704 634 Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids meiosis|mRNA methylation mRNA (N6-adenosine)-methyltransferase activity cellular_component YAL047C SPC72 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0281591126688279 0.102890093913156 635 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization microtubule nucleation|nuclear migration along microtubule|mitotic cell cycle spindle orientation checkpoint|mitotic spindle elongation|mitotic sister chromatid segregation structural constituent of cytoskeleton outer plaque of spindle pole body YKL190W CNB1 hom FF|FT SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS 0.0280453707767481 0.104288240674132 636 Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|fungal-type cell wall organization|cellular ion homeostasis calcium-dependent protein serine/threonine phosphatase activity|calcium ion binding calcineurin complex YDR128W MTC5 hom 0.0280273806976772 0.104666011368352 637 Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress biological_process molecular_function fungal-type vacuole membrane|Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YGL031C RPL24A hom FT TRANSLATION|RIBOSOME 0.0279634070638025 0.105305080718378 638 Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YKL160W ELF1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS 0.0279338141350336 0.105726142624462 639 Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression regulation of transcription by chromatin organization|transcription elongation from RNA polymerase II promoter RNA polymerase II core binding transcription elongation factor complex|nucleus YCR024C-A PMP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0279004030932161 0.106092039771018 640 Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication cation transport enzyme regulator activity plasma membrane|membrane YDL084W SUB2 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0278507330260488 0.106715730177004 641 Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|mRNA splicing, via spliceosome|transcription elongation from RNA polymerase II promoter|chromatin silencing at telomere ATP-dependent RNA helicase activity chromosome, telomeric region|nucleus|transcription export complex|spliceosomal complex YNR023W SNF12 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RSC COMPLEX 0.0278289648316611 0.107251869526881 642 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YBL090W MRP21 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0278097666611084 0.107232313775207 643 Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial translational initiation|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YJL158C CIS3 hom FT CELL WALL ORG/BIOGENESIS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0277561905056383 0.10791089188717 644 Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family fungal-type cell wall organization structural constituent of cell wall extracellular region|fungal-type cell wall|endoplasmic reticulum|plasma membrane|cellular bud tip YIL157C COA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0277479716596496 0.108015289074462 645 Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly mitochondrial respiratory chain complex IV assembly molecular_function integral to mitochondrial membrane|mitochondrion|integral to mitochondrial inner membrane YBR264C YPT10 hom FT PROTEIN LOCALIZATION|SIGNALING|PLASMA MEMBRANE 0.0277310667608238 0.108230269209314 646 Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Golgi organization GTPase activity|guanyl nucleotide binding cytoplasm YOR078W BUD21 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0276626449116663 0.109103851477894 647 Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|90S preribosome YLR251W SYM1 hom FT MITOCHONDRION 0.0276260609495465 0.109573222144681 648 Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 ethanol metabolic process molecular_function mitochondrion|integral to membrane|mitochondrial inner membrane YDR368W YPR1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0276077551979236 0.109808682310892 649 NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication cellular response to oxidative stress|D-xylose catabolic process|arabinose catabolic process glucose 1-dehydrogenase (NADP+) activity|alditol:NADP+ 1-oxidoreductase activity|carbonyl reductase (NADPH) activity nucleus|cytoplasm YFR018C_p YFR018C hom FT PROTEOLYSIS 0.0275992850461967 0.109917765937432 650 Putative protein of unknown function biological_process molecular_function cellular_component YDR498C SEC20 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.027579743417574 0.110275925994548 651 Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p retrograde vesicle-mediated transport, Golgi to ER|vesicle fusion with endoplasmic reticulum SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum|peroxisome YPL207W TYW1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0274932000679817 0.111291252195795 652 Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron wybutosine biosynthetic process molecular_function endoplasmic reticulum YDL202W MRPL11 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0274518675615937 0.111830036973655 653 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOR304C-A_p YOR304C-A hom FT SITE OF POLARIZED GROWTH 0.0274400505314191 0.112198561697714 654 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck biological_process molecular_function cellular bud|mating projection tip|cellular bud neck|cytoplasm YPL073C_d YPL073C hom 0.0273748763757518 0.112839127857025 655 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Unknown Unknown Unknown YMR010W_p YMR010W hom 0.0273722863426858 0.112873198631477 656 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function integral to membrane|cytoplasm YBR084W MIS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0273547242372682 0.113104434422064 657 Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase nucleobase-containing compound metabolic process|folic acid biosynthetic process formate-tetrahydrofolate ligase activity|methylenetetrahydrofolate dehydrogenase (NADP+) activity|methenyltetrahydrofolate cyclohydrolase activity mitochondrion YLR252W_d YLR252W hom 0.02733865619287 0.113316324982865 658 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Unknown Unknown Unknown YKL086W SRX1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0272774254190806 0.114126646461979 659 Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress cellular response to oxidative stress sulfiredoxin activity nucleus|cytoplasm YHR175W-A_p YHR175W-A hom 0.0272742975494994 0.114168162447266 660 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YJR107W_p YJR107W hom FT LIPID METABOLISM 0.0272667766542478 0.11426803538137 661 Putative lipase biological_process lipase activity cellular_component YOR164C GET4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION 0.0272378499666621 0.114652805033893 662 Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 protein insertion into ER membrane|posttranslational protein targeting to membrane molecular_function TRC complex|cytoplasm YBR085C-A YBR085C-A hom FT NUCLEUS 0.0272181336360787 0.11491564617388 663 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YEL059W_d HHY1 hom 0.0271949594015142 0.115225190601643 664 Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Unknown Unknown Unknown YPR016C TIF6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS 0.0271682227252138 0.115583133451266 665 Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits ribosomal subunit export from nucleus|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing ribosomal large subunit binding nucleolus|nucleus|preribosome, large subunit precursor|cytoplasm YPL213W LEA1 hom FT RNA PROCESSING|NUCLEUS 0.0271535549365829 0.115779872399919 666 Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein mRNA splicing, via spliceosome molecular_function U2 snRNP|U2-type prespliceosome|cytoplasm YGL066W SGF73 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RNA LOCALIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0271272579282099 0.116133252629145 667 SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay histone deubiquitination|mRNA export from nucleus|histone acetylation|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|protein complex assembly|chromatin modification histone acetyltransferase activity|enzyme activator activity|structural molecule activity DUBm complex|SLIK (SAGA-like) complex|SAGA complex YMR055C BUB2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON 0.0271251078230936 0.116162183251159 668 Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage regulation of exit from mitosis|mitotic cell cycle spindle orientation checkpoint GTPase activator activity spindle pole body YNL120C_d YNL120C hom 0.0271073954687293 0.116400726313545 669 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Unknown Unknown Unknown YCR092C MSH3 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0271051970776153 0.116430360201323 670 Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability mismatch repair|mitotic recombination|meiotic mismatch repair|removal of nonhomologous ends|DNA recombination DNA insertion or deletion binding|mismatched DNA binding|double-strand/single-strand DNA junction binding|loop DNA binding|Y-form DNA binding MutSbeta complex|nucleus|cytoplasm YML103C NUP188 hom FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0270755285383324 0.116830866110166 671 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188 nuclear pore organization|protein import into nucleus|mRNA export from nucleus in response to heat stress structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YER025W GCD11 het FT TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.027027218448911 0.117485334042506 672 Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met positive regulation of translational fidelity GTP binding|tRNA binding|translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YCL045C EMC1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0270164206194511 0.117632007271841 673 Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YDL134C PPH21 hom FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0270057930960934 0.117995748334905 674 Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication actin filament organization|budding cell bud growth|protein dephosphorylation|G1/S transition of mitotic cell cycle|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity protein phosphatase type 2A complex YIR021W-A_p YIR021W-A hom 0.0270037422759421 0.117804407917568 675 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YAL029C MYO4 hom FT RNA LOCALIZATION|MITOCHONDRION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0270005563011102 0.117847762077563 676 Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication mating type switching|endoplasmic reticulum inheritance|intracellular mRNA localization microfilament motor activity|actin filament binding mitochondrion|filamentous actin|myosin V complex|cellular bud|cellular bud tip YMR326C_d YMR326C hom 0.026978629401718 0.118146478789666 677 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Unknown Unknown Unknown YOR380W RDR1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0269541129504447 0.118756055576809 678 Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to xenobiotic stimulus sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YLR077W FMP25 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0269522688598787 0.118506381839427 679 Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|integral to mitochondrial inner membrane YKL013C ARC19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.026940260011164 0.118670624471411 680 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin cortical patch assembly structural molecule activity Arp2/3 protein complex YIL124W AYR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0269082712428091 0.119164052679987 681 NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones phosphatidic acid biosynthetic process acylglycerone-phosphate reductase activity mitochondrial outer membrane|mitochondrion|integral to membrane|endoplasmic reticulum|lipid particle|cytoplasm YJL085W EXO70 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0268949898707346 0.1195671235295 682 Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Golgi to plasma membrane transport|exocytosis|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YER031C YPT31 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS 0.0268891381884847 0.119426913945253 683 Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication early endosome to Golgi transport|vesicle-mediated transport|exocytosis GTPase activity mitochondrial outer membrane|endosome|Golgi apparatus YLR107W REX3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.026876441097278 0.119601604648301 684 RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases U5 snRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing 3'-5'-exoribonuclease activity|3'-5' exonuclease activity nucleus|cytoplasm YML030W RCF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0268388452069127 0.120120034611581 685 Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast mitochondrial respiratory chain complex IV assembly|mitochondrial respiratory chain supercomplex assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|integral to mitochondrial inner membrane|mitochondrial respiratory chain complex IV YKL055C OAR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0267703151372475 0.121014099791953 686 Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p fatty acid metabolic process|aerobic respiration 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity mitochondrion YIL069C RPS24B hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.0267678331167913 0.12110405536584 687 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YDL133C-A RPL41B hom FT TRANSLATION|RIBOSOME 0.0267608260731905 0.121146009675023 688 Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL201W TRM8 hom FT RNA PROCESSING|NUCLEUS 0.0267482804762301 0.121320581757928 689 Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YOR154W_p SLP1 hom 0.026732129483468 0.121545612463724 690 Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p protein folding in endoplasmic reticulum molecular_function cellular_component YHL017W_p YHL017W hom FT GOLGI APPARATUS 0.0267141453855415 0.121796567478357 691 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication biological_process molecular_function integral to membrane|clathrin-coated vesicle YBL054W TOD6 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|CHROMOSOME 0.0267040932559914 0.121937013959221 692 PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|Rpd3L complex|cytoplasm YLR166C SEC10 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0266608916471099 0.122542059939674 693 Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion exocytosis|establishment of cell polarity|Golgi to plasma membrane transport|vesicle fusion|vesicle docking involved in exocytosis molecular_function mating projection tip|exocyst YLR118C YLR118C hom FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.0266515207661552 0.122673609679949 694 Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS protein deacylation palmitoyl-(protein) hydrolase activity nucleus|cytoplasm YPL183C RTT10 hom FT VESICLE-MEDIATED TRANSPORT 0.0266169598657482 0.123327520884463 695 WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endocytic recycling molecular_function endosome|cytoplasm YJL054W TIM54 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0266099417575191 0.123258632253675 696 Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane protein import into mitochondrial inner membrane protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YLR020C YEH2 hom FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0265195822961536 0.124537509274155 697 Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism cell wall mannoprotein biosynthetic process|sterol metabolic process sterol esterase activity integral to membrane|plasma membrane YLR312W-A MRPL15 hom FT RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0265116091460999 0.124819548607024 698 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YMR031C EIS1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0264576865188803 0.125588651033993 699 Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress eisosome assembly molecular_function eisosome|mitochondrion|membrane raft|cytoplasm YDR003W-A_p YDR003W-A hom 0.0264338276709007 0.125760757801911 700 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YKR027W BCH2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.0263551404269579 0.126891406702163 701 Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function trans-Golgi network transport vesicle|mitochondrion|clathrin-coated vesicle|exomer complex YHR077C NMD2 hom 0.0263420844746805 0.127079768902999 702 Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|translational frameshifting molecular_function polysome|cytoplasm YHR106W TRR2 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.026293459907568 0.127783204267042 703 Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication cellular response to oxidative stress|cell redox homeostasis thioredoxin-disulfide reductase activity mitochondrion YJR145C RPS4A hom FT TRANSLATION|RIBOSOME 0.0262367391415707 0.128778643893826 704 Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasm|90S preribosome YPR032W SRO7 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|PLASMA MEMBRANE 0.0262246740593524 0.128783471187601 705 Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication establishment of cell polarity|exocytosis|Golgi to plasma membrane transport|small GTPase mediated signal transduction SNARE binding|Rab GTPase binding cytosol|plasma membrane YPL226W NEW1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|NUCLEUS|MITOCHONDRION 0.0262157847854222 0.128913179344296 706 ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction ribosomal small subunit biogenesis|poly(A)+ mRNA export from nucleus ATPase activity|ATP binding mitochondrion|polysome|cytoplasm YMR101C SRT1 hom FT CARBOHYDRATE METABOLISM 0.0262070081218326 0.129384221477913 707 Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase protein glycosylation dehydrodolichyl diphosphate synthase activity|prenyltransferase activity lipid particle YJL102W MEF2 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0261022100662494 0.130579351180968 708 Mitochondrial elongation factor involved in translational elongation translational elongation translation elongation factor activity mitochondrion YBR270C_p BIT2 hom 0.0260996539452198 0.130617041409271 709 Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication biological_process molecular_function cellular_component YBR049C REB1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0260767353148469 0.130955355622502 710 RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication termination of RNA polymerase I transcription|regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nucleoplasm YKR084C HBS1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM 0.0260611524246715 0.131185770705452 711 GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay nuclear-transcribed mRNA catabolic process, no-go decay|ribosome disassembly|nonfunctional rRNA decay ribosome binding|GTPase activity cytoplasm YKL011C CCE1 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0260419678156312 0.131469873691936 712 Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA mitochondrial genome maintenance endodeoxyribonuclease activity mitochondrial inner membrane YPL044C_d YPL044C het 0.0260350913246461 0.131629385124676 713 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Unknown Unknown Unknown YOR311C DGK1 hom FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0260327688437176 0.131663838063893 714 Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain phosphatidic acid biosynthetic process diacylglycerol kinase activity integral to endoplasmic reticulum membrane|integral to membrane YBR045C GIP1 hom FT CELL WALL ORG/BIOGENESIS 0.0260012631671376 0.132074243432209 715 Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p ascospore wall assembly|regulation of phosphoprotein phosphatase activity protein phosphatase type 1 regulator activity|protein phosphatase 1 binding protein phosphatase type 1 complex|prospore membrane YAL040C CLN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0260011163614224 0.132134084828113 716 G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis vacuole organization|regulation of cyclin-dependent protein kinase activity|regulation of transcription involved in G1/S phase of mitotic cell cycle|vacuole fusion, non-autophagic|regulation of cell size|G1/S transition of mitotic cell cycle cyclin-dependent protein kinase regulator activity nucleus YGL058W RAD6 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|GENE SILENCING|NUCLEUS|CHROMOSOME 0.025963142596182 0.132642194545529 717 Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p protein ubiquitination involved in ubiquitin-dependent protein catabolic process|histone monoubiquitination|protein monoubiquitination|error-free translesion synthesis|error-prone translesion synthesis|transcription from RNA polymerase II promoter|mitotic cell cycle G1/S transition DNA damage checkpoint|regulation of dipeptide transport|meiotic DNA double-strand break formation|ubiquitin-dependent protein catabolic process via the N-end rule pathway|double-strand break repair via homologous recombination|protein polyubiquitination|error-free postreplication DNA repair|chromatin silencing at telomere ubiquitin-protein ligase activity nuclear chromatin|proteasome complex|nucleus|cytoplasm YPL102C_d YPL102C hom 0.0259406685645652 0.132977915566009 718 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Unknown Unknown Unknown YKR054C DYN1 hom FT CELL CYCLE|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.025917688469033 0.133321875771888 719 Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p nuclear migration along microtubule|establishment of mitotic spindle orientation|mitotic spindle elongation|mitotic sister chromatid segregation microtubule motor activity cytoplasmic dynein complex|cell cortex|cytoplasmic microtubule|spindle pole body|astral microtubule YDR049W VMS1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0259172770026981 0.133328040760958 720 Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans ER-associated protein catabolic process|mitochondria-associated protein catabolic process|nucleus-associated proteasomal ubiquitin-dependent protein catabolic process molecular_function cytosol|mitochondrion|endoplasmic reticulum membrane|Cdc48p-Npl4p-Vms1p AAA ATPase complex|cytoplasm YOR166C SWT1 hom FT NUCLEUS 0.0258684541048538 0.134061119649463 721 RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain nuclear mRNA surveillance of mRNP export|transcription, DNA-dependent endoribonuclease activity nucleus|cytoplasm YNL133C FYV6 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0258666821878518 0.134087783574511 722 Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining double-strand break repair via nonhomologous end joining molecular_function nucleus YLL038C ENT4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.025828210825908 0.134667714550466 723 Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome endocytosis|actin filament organization|establishment of protein localization to plasma membrane|actin cortical patch assembly clathrin binding actin cortical patch YPL040C ISM1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0258126125952674 0.1349033992503 724 Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth isoleucyl-tRNA aminoacylation|mitochondrial isoleucyl-tRNA aminoacylation|mitochondrial translation isoleucine-tRNA ligase activity mitochondrion YIL016W SNL1 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.025799651908762 0.135099473698297 725 Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein protein folding|nuclear pore organization ribosome binding nuclear envelope|mitochondrion|cytosolic ribosome|endoplasmic reticulum membrane|membrane YJL003W COX16 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0257896523098029 0.135250901997011 726 Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|mitochondrial inner membrane YLR359W ADE13 het FT NUCLEOTIDE METABOLISM 0.025787965637674 0.135276456924721 727 Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity cellular_component YPR098C YPR098C hom FT MITOCHONDRION 0.0257745231133491 0.135480259173294 728 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion YPR067W ISA2 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0257741089988906 0.135486541328859 729 Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations iron-sulfur cluster assembly|biotin biosynthetic process iron ion binding mitochondrion|mitochondrial intermembrane space YKL059C MPE1 het FT RNA PROCESSING|NUCLEUS 0.0257605989882101 0.135691612853928 730 Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YNR053C NOG2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.0257443559380693 0.136113696036755 731 Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation ribosomal large subunit export from nucleus GTPase activity nucleoplasm|nucleolus|nucleus|preribosome, large subunit precursor YOR366W_d YOR366W hom 0.0256961488870257 0.136673215247221 732 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Unknown Unknown Unknown YDL116W NUP84 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0256639907523452 0.137165041053726 733 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 double-strand break repair|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|maintenance of chromatin silencing at telomere|nuclear pore distribution|telomere tethering at nuclear periphery|response to DNA damage stimulus|mRNA export from nucleus in response to heat stress|mRNA export from nucleus|positive regulation of transcription, DNA-dependent|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette structural constituent of nuclear pore integral to membrane|nuclear pore outer ring|nuclear pore YCR001W_d YCR001W hom 0.025644554632416 0.137462957537171 734 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Unknown Unknown Unknown YER028C MIG3 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.025596718005945 0.13819832065532 735 Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication cellular response to UV|negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage|response to DNA damage stimulus RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nuclear chromatin YOR267C HRK1 hom FT ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0255803993669617 0.138449869040355 736 Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress protein phosphorylation|cellular ion homeostasis protein serine/threonine kinase activity|protein kinase activity cytoplasm YOR171C LCB4 hom FT LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0255721283697898 0.138577499295338 737 Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes calcium-mediated signaling|sphingolipid metabolic process D-erythro-sphingosine kinase activity Golgi apparatus|endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum YIL107C PFK26 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0255704224594376 0.138603834566954 738 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YGR146C ECL1 hom 0.0255519600885761 0.13888909704529 739 Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function cellular_component YHR091C MSR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0255063680218022 0.139595477015268 740 Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 arginyl-tRNA aminoacylation|mitochondrial arginyl-tRNA aminoacylation|mitochondrial translation arginine-tRNA ligase activity mitochondrion YJL133W MRS3 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0254382661429857 0.140655755782926 741 Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication mitochondrial iron ion transport|RNA splicing iron ion transmembrane transporter activity mitochondrion|integral to membrane YIL031W ULP2 het FT CELL CYCLE|PROTEOLYSIS|NUCLEUS 0.0254292426665592 0.140796705542148 742 Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate plasmid maintenance|protein desumoylation|chromosome condensation|mitotic cell cycle spindle assembly checkpoint cysteine-type peptidase activity|SUMO-specific protease activity nucleus YGL114W_p YGL114W hom FT PROTEIN LOCALIZATION 0.0254210014947326 0.140925530255719 743 Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters transport oligopeptide transporter activity integral to membrane|membrane YLR071C RGR1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.025399370276551 0.141264096960791 744 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding core mediator complex YNL073W MSK1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION|MITOCHONDRION 0.02539740002351 0.141294965916985 745 Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) tRNA import into mitochondrion|lysyl-tRNA aminoacylation|mitochondrial lysyl-tRNA aminoacylation|mitochondrial translation lysine-tRNA ligase activity mitochondrion YLR184W_d YLR184W hom 0.02537390777925 0.14166343019741 746 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCL058C FYV5 hom FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS 0.0253162622165452 0.142630247003546 747 Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis pheromone-dependent signal transduction involved in conjugation with cellular fusion|cellular ion homeostasis molecular_function integral to membrane YGR062C COX18 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0252955726041571 0.142897412546969 748 Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC protein insertion into mitochondrial membrane from inner side membrane insertase activity integral to membrane|integral to mitochondrial inner membrane YCL039W GID7 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.0252773792959312 0.143423961005174 749 Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process molecular_function nucleus|GID complex|cytoplasm YLR015W BRE2 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0252475464054427 0.143658016953447 750 Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L histone H3-K4 methylation|telomere maintenance|histone H3-K4 trimethylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YNL315C ATP11 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0252135726764132 0.144197939987856 751 Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase mitochondrion organization|mitochondrial proton-transporting ATP synthase complex assembly unfolded protein binding mitochondrion|mitochondrial matrix YAL023C PMT2 hom FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0251744627301969 0.14482141338365 752 Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication ER-associated misfolded protein catabolic process|protein O-linked glycosylation|Unknown|protein exit from endoplasmic reticulum|protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YOR295W UAF30 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0251657405377303 0.145140683428746 753 Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication transcription of nuclear large rRNA transcript from RNA polymerase I promoter|chromatin organization RNA polymerase I upstream control element sequence-specific DNA binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YIL046W MET30 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0251316160204711 0.145566868639092 754 F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus response to cadmium ion|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|regulation of DNA-dependent DNA replication initiation|M phase of mitotic cell cycle|response to arsenic-containing substance|G1/S transition of mitotic cell cycle|protein polyubiquitination|regulation of transcription involved in G1 phase of mitotic cell cycle|protein ubiquitination ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus YPL188W POS5 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0251304158247072 0.145526059055972 755 Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress cellular response to oxidative stress|NADP biosynthetic process NADH kinase activity mitochondrion|mitochondrial matrix YHR158C KEL1 hom FT CELL CYCLE|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH 0.0251016602083519 0.145987494265174 756 Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate negative regulation of exit from mitosis|regulation of cell shape|cell morphogenesis|cytogamy|plasma membrane fusion molecular_function mating projection tip|cellular bud neck|cytoplasm|cellular bud tip YNL235C_d YNL235C hom 0.0250595369767467 0.146665456434838 757 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Unknown Unknown Unknown YKR103W NFT1 hom FT NUCLEOTIDE METABOLISM 0.0250570279130638 0.146766158428601 758 Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YNL124W NAF1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0250240736624805 0.147238093226364 759 RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p box H/ACA snoRNP assembly RNA binding nucleoplasm|nucleus YKL068W-A_p YKL068W-A hom 0.0250240222201132 0.147238925120965 760 Putative protein of unknown function; identified by homology to Ashbya gossypii biological_process molecular_function cellular_component YHR006W STP2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|PLASMA MEMBRANE 0.0250154903977185 0.147376946256047 761 Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YLR275W SMD2 het FT RNA PROCESSING|NUCLEUS 0.025007022240789 0.148058426663216 762 Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 mRNA splicing, via spliceosome molecular_function U1 snRNP|U4/U6 x U5 tri-snRNP complex|U2-type prespliceosome|U5 snRNP YOL151W GRE2 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0249982034105262 0.147656904642189 763 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress ergosterol metabolic process|filamentous growth methylglyoxal reductase (NADPH-dependent) activity|3-methylbutanol:NAD(P) oxidoreductase activity nucleus|cytoplasm YNL257C SIP3 hom FT TRANSCRIPTION FROM RNA POL II 0.0249887994433422 0.147809370030009 764 Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter transcription cofactor activity integral to membrane YOL068C HST1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0249857868773632 0.147858237822057 765 NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance chromatin silencing|negative regulation of mitotic recombination|histone deacetylation NAD-independent histone deacetylase activity|NAD-dependent histone deacetylase activity Set3 complex YMR228W MTF1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0249445791442824 0.148527921009987 766 Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation transcription initiation from mitochondrial promoter mitochondrial RNA polymerase binding promoter specificity activity mitochondrion|mitochondrial DNA-directed RNA polymerase complex|mitochondrial matrix|mitochondrial intermembrane space YGR055W MUP1 hom FT PLASMA MEMBRANE 0.024910798793892 0.149929990941525 767 High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake cysteine transport|sulfur amino acid transport|methionine transport L-methionine secondary active transmembrane transporter activity integral to membrane|integral to plasma membrane YOR162C YRR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0248741763395528 0.149677418379219 768 Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter in response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YOR214C_p YOR214C hom 0.0248553444757396 0.14998604152108 769 Putative protein of unknown function; YOR214C is not an essential gene biological_process molecular_function fungal-type cell wall YDR482C CWC21 hom FT RNA PROCESSING|NUCLEUS 0.0248445987475114 0.150223167794866 770 Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YDR509W_d YDR509W hom 0.0248147870309016 0.150652358273589 771 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR082W STN1 het FT NUCLEUS|CHROMOSOME 0.0247902483204351 0.151239520259517 772 Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping telomere capping|negative regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YBR152W SPP381 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.024780625092587 0.151215351446719 773 mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step molecular_function U4/U6 x U5 tri-snRNP complex YNL198C_d YNL198C hom 0.024743548407101 0.151828191641033 774 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR005W SSL1 het FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0247408589065631 0.152056028458877 775 Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p phosphorylation of RNA polymerase II C-terminal domain|translational initiation|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YOL149W DCP1 het FT RNA PROCESSING 0.0247216342069906 0.1521912990916 776 Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA mRNA binding|enzyme activator activity|m7G(5')pppN diphosphatase activity cytoplasmic mRNA processing body YGR146C-A_p YGR146C-A hom 0.0247113681136978 0.152361630291116 777 Putative protein of unknown function biological_process molecular_function cellular_component YDR004W RAD57 hom FF|FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0247087216072005 0.152405563646486 778 Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p DNA recombinase assembly|telomere maintenance via recombination|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YDR337W MRPS28 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0247086265538736 0.152468300538926 779 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome|RNA binding mitochondrion|mitochondrial small ribosomal subunit YMR068W AVO2 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0246173334869757 0.153928572390873 780 Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|cytoplasm YDR127W ARO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0245971012074534 0.155439559867572 781 Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids aromatic amino acid family biosynthetic process shikimate kinase activity|shikimate 3-dehydrogenase (NADP+) activity|3-phosphoshikimate 1-carboxyvinyltransferase activity|3-dehydroquinate dehydratase activity|3-dehydroquinate synthase activity cytoplasm YPR171W BSP1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0245721985953468 0.155054492463274 782 Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton actin cortical patch localization protein binding, bridging mating projection tip|membrane|cellular bud neck|actin cortical patch|cellular bud tip YDL160C-A MHF2 hom FT RESPONSE TO DNA DAMAGE 0.024554172892094 0.154987900086887 783 Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 response to DNA damage stimulus molecular_function cellular_component YBR221C PDB1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0245292470318638 0.15540747541556 784 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria acetyl-CoA biosynthetic process from pyruvate pyruvate dehydrogenase (acetyl-transferring) activity mitochondrial nucleoid|mitochondrion|mitochondrial pyruvate dehydrogenase complex YOL133W HRT1 het FT CELL CYCLE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0245202853301889 0.155558537592293 785 RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity SCF ubiquitin ligase complex|nucleus|cytoplasm YHL035C VMR1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0244861547738718 0.156134876653901 786 Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions transport|response to drug|response to metal ion|drug transport ATPase activity, coupled to transmembrane movement of substances fungal-type vacuole membrane|mitochondrion|integral to membrane|ribosome YDR417C_d YDR417C hom 0.0244776089504401 0.156279437209722 787 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Unknown Unknown Unknown YGL240W DOC1 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS|NUCLEUS|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.0244264486678964 0.157146984031431 788 Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain activation of mitotic anaphase-promoting complex activity|chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|cyclin catabolic process enzyme regulator activity|mitotic anaphase-promoting complex activity mitochondrion|anaphase-promoting complex YKR085C MRPL20 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0244204369446024 0.157249166497385 789 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR289W GUF1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION|RIBOSOME 0.0244079828784251 0.157461010892422 790 Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor positive regulation of translation ribosome binding|GTPase activity mitochondrion|mitochondrial ribosome|mitochondrial matrix YKL084W HOT13 hom FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0243040091333995 0.159238053679858 791 Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein import into mitochondrial intermembrane space zinc ion binding mitochondrial intermembrane space YCR071C IMG2 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0243002883294312 0.159675549373118 792 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOR037W CYC2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0242419035954937 0.16030672734771 793 Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) cytochrome c-heme linkage|mitochondrial membrane organization oxidoreductase activity extrinsic to mitochondrial inner membrane|mitochondrion YJL112W MDV1 hom FT MITOCHONDRION ORGANIZATION|PEROXISOME ORGANIZATION|MITOCHONDRION 0.0242045419310325 0.16095222912668 794 Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication peroxisome fission|mitochondrial fission|mitochondrial genome maintenance ubiquitin binding mitochondrial outer membrane|mitochondrion YDR493W MZM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0241934593654969 0.16114408083963 795 Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog mitochondrial respiratory chain complex III assembly molecular_function mitochondrion|mitochondrial matrix YMR132C_p JLP2 hom 0.024181741905557 0.16178517271941 796 Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) biological_process molecular_function cytoplasm YHR049W FSH1 hom FT NUCLEUS 0.0241667920597517 0.161606429496273 797 Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process hydrolase activity nucleus|cytoplasm YNL339C YRF1-6 hom FT NUCLEUS 0.0241640199147788 0.161717090747248 798 Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p telomere maintenance via recombination DNA helicase activity nucleus YOR374W ALD4 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0241629767899781 0.161672659124047 799 Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed NADPH regeneration|ethanol metabolic process|pyruvate metabolic process|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity|aldehyde dehydrogenase (NAD) activity mitochondrial nucleoid|mitochondrion YHR074W QNS1 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0241413807044301 0.162047933778233 800 Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide NAD biosynthetic process NAD+ synthase (glutamine-hydrolyzing) activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides nucleus|cytoplasm YGR257C MTM1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0241243541219359 0.162406906901546 801 Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor manganese ion transport|transport transporter activity|metallochaperone activity mitochondrion|integral to membrane|mitochondrial inner membrane YHR082C KSP1 hom FT PROTEIN LOCALIZATION|SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0241116818464145 0.162565082875895 802 Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress TOR signaling cascade|protein phosphorylation|Unknown|cellular protein localization|regulation of filamentous growth|negative regulation of macroautophagy protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YDL154W MSH5 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0240945313027803 0.162864293277373 803 Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans reciprocal meiotic recombination molecular_function nucleus YNL286W CUS2 hom FT RNA PROCESSING|NUCLEUS 0.0240836788602027 0.16343069434559 804 Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) mRNA splicing, via spliceosome RNA binding U2 snRNP YBR017C KAP104 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SITE OF POLARIZED GROWTH 0.0240729260757076 0.163241811307355 805 Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis protein import into nucleus|cell cycle|mRNA-binding (hnRNP) protein import into nucleus|transcription factor import into nucleus nuclear localization sequence binding cytosol|integral to membrane|cellular bud neck|cellular bud tip YOR025W HST3 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0240277531366428 0.164033269152549 806 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|histone deacetylation|chromatin silencing at telomere histone deacetylase activity|DNA binding nucleus YJL081C ARP4 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0240221965888535 0.164760973875121 807 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes DNA repair|histone acetylation|kinetochore assembly|chromatin organization|regulation of transcription, DNA-dependent|regulation of transcription from RNA polymerase II promoter|chromatin remodeling chromatin binding|histone acetyltransferase activity|ATP-dependent 3'-5' DNA helicase activity nuclear chromatin|Swr1 complex|nucleus|Ino80 complex|NuA4 histone acetyltransferase complex YER185W PUG1 hom FT PLASMA MEMBRANE 0.0240048275434098 0.164436043584465 808 Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins heme transport molecular_function integral to membrane|plasma membrane YOR376W-A_p YOR376W-A hom 0.0240034033126157 0.164461090107654 809 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YJL156C SSY5 het FT PROTEOLYSIS|PLASMA MEMBRANE 0.0239443874208276 0.165564551571869 810 Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p protein processing|response to amino acid stimulus serine-type endopeptidase activity extrinsic to plasma membrane YNR069C BSC5 hom 0.0239341184927311 0.165746110125352 811 Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro biological_process molecular_function cellular_component YLR400W_d YLR400W hom 0.0239278703349098 0.165793532063133 812 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL186W MNN5 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0238804506336663 0.166634178895103 813 Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment protein glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YHL004W MRP4 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0238725188523779 0.166775104025538 814 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome|tRNA binding mitochondrion|mitochondrial small ribosomal subunit YBL038W MRPL16 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0238385251397349 0.167380089711162 815 Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome|peptidyltransferase activity mitochondrion|mitochondrial large ribosomal subunit YKL040C NFU1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.0238344866770134 0.167452071562941 816 Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria cellular iron ion homeostasis|iron-sulfur cluster assembly|intracellular sequestering of iron ion molecular_function mitochondrion|mitochondrial matrix YKR056W TRM2 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE 0.0238242310813598 0.167698344050191 817 tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair double-strand break repair|DNA repair|tRNA modification tRNA (m5U54) methyltransferase activity|double-stranded DNA specific 5'-3' exodeoxyribonuclease activity|single-stranded DNA specific endodeoxyribonuclease activity cellular_component YNR016C ACC1 het FF|FT PROTEIN LOCALIZATION|KETONE METABOLISM|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0238197213727917 0.16771544727009 818 Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; nuclear envelope organization|protein import into nucleus|fatty acid biosynthetic process biotin carboxylase activity|acetyl-CoA carboxylase activity mitochondrion|endoplasmic reticulum membrane YCL027W FUS1 hom FT PROTEIN LOCALIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0238125685151358 0.168096910933664 819 Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate regulation of termination of mating projection growth|cytogamy|cortical protein anchoring molecular_function integral to plasma membrane|mating projection tip YHR098C SFB3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.0237282060501044 0.169354790188802 820 Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat|mating projection tip YML106W URA5 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0237009084607326 0.169846098903138 821 Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine ribonucleoside biosynthetic process orotate phosphoribosyltransferase activity nucleus|cytoplasm YGR286C BIO2 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0236871396052243 0.170476923803362 822 Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant biotin biosynthetic process biotin synthase activity mitochondrion YKL082C RRP14 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.0236658886590728 0.170477956853677 823 Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family ribosomal small subunit biogenesis|ribosomal large subunit biogenesis molecular_function cytosolic large ribosomal subunit|nucleolus YHL029C_p OCA5 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.0236602038858385 0.170580692376704 824 Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts biological_process molecular_function cytoplasm YMR131C RRB1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0236521002029754 0.170727222703203 825 Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p ribosome biogenesis molecular_function nucleolus YBR120C CBP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0236436972561328 0.170879263812888 826 Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex positive regulation of mitochondrial translation|mitochondrial respiratory chain complex III assembly ribosome binding mitochondrion|mitochondrial ribosome YOR139C_d YOR139C hom 0.0236018238869819 0.171638422957157 827 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Unknown Unknown Unknown YJR115W_p YJR115W hom 0.0236016570605521 0.171833237144232 828 Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication biological_process molecular_function cellular_component YOL052C-A DDR2 hom 0.0235836544367375 0.171968616790113 829 Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication response to stress molecular_function fungal-type vacuole|cytoplasm YNL136W EAF7 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0235649649826248 0.172308756124259 830 Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter molecular_function histone acetyltransferase complex|nucleus|NuA4 histone acetyltransferase complex YPL106C SSE1 hom 0.0235644312890535 0.172318476480104 831 ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication protein folding|protein refolding peptide binding|adenyl-nucleotide exchange factor activity|ATP binding polysome|cytoplasm YGR003W CUL3 hom FT PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0235530151999769 0.172590515027122 832 Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity nucleus|Cul3-RING ubiquitin ligase complex|cytoplasm YJL077W-B_p YJL077W-B hom 0.023544273207804 0.172685923249941 833 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YML037C_p YML037C hom 0.023506541742887 0.173439399236921 834 Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene biological_process molecular_function clathrin-coated vesicle YJL009W_d YJL009W het 0.0234790839880968 0.173878218904599 835 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Unknown Unknown Unknown YKR049C FMP46 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0234417713946433 0.174627691562903 836 Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process oxidoreductase activity mitochondrion YOR060C SLD7 het FT CELL CYCLE|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CYTOSKELETON 0.0234039152581622 0.175260655527314 837 Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria regulation of DNA-dependent DNA replication initiation molecular_function nuclear envelope|endoplasmic reticulum|DNA replication preinitiation complex|nucleus|spindle pole body|cytoplasm YLR285C-A_p YLR285C-A hom 0.0234028555813286 0.175344566069505 838 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YBR183W YPC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0233849775775822 0.175610229828133 839 Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication ceramide catabolic process|ceramide biosynthetic process phytoceramidase activity|sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YLR339C_d YLR339C het 0.0233147862905727 0.177298238773005 840 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Unknown Unknown Unknown YNL146C-A_p YNL146C-A hom 0.0232084850379281 0.178893172488259 841 Putative protein of unknown function biological_process molecular_function cellular_component YDR475C JIP4 hom 0.0231913490470579 0.179214333615653 842 Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence biological_process molecular_function cellular_component YJL201W ECM25 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS 0.0231780427132329 0.179464015100816 843 Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p fungal-type cell wall organization|filamentous growth molecular_function cytoplasm YER094C PUP3 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0231596478063047 0.179809604656992 844 Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activator activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YHR009C_p TDA3 hom FT VESICLE-MEDIATED TRANSPORT|OXIDATION-REDUCTION PROCESS 0.0231281590096974 0.180402339354931 845 Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 retrograde transport, endosome to Golgi molecular_function late endosome|cytoplasm YLR382C NAM2 hom FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0231254495639609 0.181235107482146 846 Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Group I intron splicing|leucyl-tRNA aminoacylation|mitochondrial translation mRNA binding|leucine-tRNA ligase activity mitochondrion YHL034C SBP1 hom FT TRANSLATION|NUCLEUS 0.023103697887622 0.180863787714108 847 Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 negative regulation of translation|negative regulation of translation in response to stress RNA binding|eukaryotic initiation factor 4G binding nucleolus|cytoplasmic mRNA processing body|cytoplasm YDR529C QCR7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0230976438128806 0.180978130303392 848 Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration|mitochondrial respiratory chain complex III assembly ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YBL079W NUP170 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0230907756886023 0.181303202531476 849 Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication nucleocytoplasmic transport|chromosome segregation|protein targeting to nuclear inner membrane|mitotic cell cycle spindle assembly checkpoint|nuclear pore complex assembly structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore inner ring YBR234C ARC40 het FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0230571141490715 0.181744991256853 850 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin nucleation|actin filament organization ubiquitin binding Arp2/3 protein complex YGL129C RSM23 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0230415528194633 0.18204006622997 851 Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YKL113C RAD27 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0230237483494908 0.182378111297385 852 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family double-strand break repair via nonhomologous end joining|replicative cell aging|DNA replication, removal of RNA primer|base-excision repair, base-free sugar-phosphate removal|gene conversion at mating-type locus, DNA repair synthesis 5'-3' exonuclease activity|5'-flap endonuclease activity mitochondrion|nucleus YER099C PRS2 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 0.0229793545754853 0.183746301331347 853 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YPR130C_d YPR130C hom 0.02295682422306 0.183652929851445 854 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL019W-A_p YOL019W-A hom 0.0229509717573094 0.184091882478061 855 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YGL121C GPG1 hom FT SIGNALING 0.0229253543624568 0.184254665806664 856 Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing signal transduction|invasive growth in response to glucose limitation signal transducer activity cellular_component YMR105C PGM2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0229176749914636 0.184729244923256 857 Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress glucose 6-phosphate metabolic process|cellular calcium ion homeostasis|galactose catabolic process|cellular cation homeostasis|glucose 1-phosphate metabolic process|trehalose biosynthetic process|UDP-glucose metabolic process|glycogen biosynthetic process phosphoglucomutase activity cytoplasm YMR103C_d YMR103C hom 0.0229156337958335 0.184440827596645 858 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR253W NAT5 hom FT RIBOSOME 0.0228815791014586 0.185094118110322 859 Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatA complex|cytosolic ribosome YLR310C CDC25 het FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEUS|PLASMA MEMBRANE 0.0228808323318625 0.185370745085194 860 Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 regulation of cell cycle|replicative cell aging|Ras protein signal transduction|traversing start control point of mitotic cell cycle Ras guanyl-nucleotide exchange factor activity cytosol|nucleus|plasma membrane|cytoplasm YDR166C SEC5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.0227791071504897 0.187070214527627 861 Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Golgi to plasma membrane transport|endoplasmic reticulum inheritance|exocytosis|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|exocyst|cellular bud tip YGL035C MIG1 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0227486493770147 0.187660562071567 862 Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion negative regulation of transcription from RNA polymerase II promoter by glucose|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|nuclear envelope lumen|cytoplasm YPL153C RAD53 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEOTIDE METABOLISM|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0226150340437523 0.190266606007763 863 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication DNA repair|protein localization|protein phosphorylation|nucleobase-containing compound metabolic process|DNA damage checkpoint|DNA replication initiation|deoxyribonucleoside triphosphate biosynthetic process protein serine/threonine/tyrosine kinase activity|DNA replication origin binding|protein kinase activity nucleus YGL159W_p YGL159W hom 0.0226100931688694 0.190628066835884 864 Putative protein of unknown function; deletion mutant has no detectable phenotype biological_process molecular_function cellular_component YDR186C YDR186C hom 0.0226085090967574 0.190394547941765 865 Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function ribosome|cytoplasm YNL127W FAR11 hom FT CELL CYCLE|SIGNALING 0.0225868101375631 0.190820479151062 866 Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YOR221C MCT1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0225317996965628 0.19190342591971 867 Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling fatty acid metabolic process|aerobic respiration [acyl-carrier-protein] S-malonyltransferase activity mitochondrion YLR116W MSL5 het FT RNA PROCESSING|NUCLEUS 0.022480729581576 0.192912840380749 868 Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA mRNA splicing, via spliceosome pre-mRNA branch point binding commitment complex YIR029W DAL2 hom 0.0224791044831138 0.192945024781675 869 Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process allantoicase activity cellular_component YBR244W GPX2 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0224734699344203 0.193056645220429 870 Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress cellular response to oxidative stress phospholipid-hydroperoxide glutathione peroxidase activity|glutathione peroxidase activity extrinsic to mitochondrial inner membrane|extrinsic to mitochondrial outer membrane|nucleus|cytoplasm YML077W BET5 het FT VESICLE-MEDIATED TRANSPORT|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0223835001252091 0.194845375812823 871 Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YJR151W-A_p YJR151W-A hom 0.022323802242726 0.196305874225341 872 Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis biological_process molecular_function cellular_component YMR304C-A_d YMR304C-A hom 0.0223195643020266 0.196123878700357 873 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Unknown Unknown Unknown YER040W GLN3 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0223172449961434 0.196170372278412 874 Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source nitrogen catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity cytosol|nucleus YIL003W CFD1 het FT RNA PROCESSING|COFACTOR METABOLISM 0.0223129094996842 0.196257304730571 875 Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol iron-sulfur cluster assembly|tRNA wobble uridine modification 4 iron, 4 sulfur cluster binding|ATPase activity cytoplasm YEL011W GLC3 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0222874545902423 0.196768278528237 876 Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress glycogen biosynthetic process 1,4-alpha-glucan branching enzyme activity cytoplasm YLR017W MEU1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0222715573436319 0.197087888419814 877 Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression L-methionine salvage from methylthioadenosine|glutamate biosynthetic process S-methyl-5-thioadenosine phosphorylase activity|mRNA binding cytoplasm YLR446W_p YLR446W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.022252345564358 0.197541478045708 878 Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene biological_process molecular_function cellular_component YJR144W MGM101 hom FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|MITOCHONDRION|CHROMOSOME 0.022232934671883 0.198066669743708 879 Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage DNA repair|recombinational repair|DNA strand renaturation molecular_function mitochondrial nucleoid|mitochondrion YMR284W YKU70 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME 0.0221266053752207 0.200220973917883 880 Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair chromatin assembly or disassembly|chromatin silencing|double-strand break repair via nonhomologous end joining|telomere maintenance|double-strand break repair via break-induced replication|double-strand break repair via homologous recombination|chromatin silencing at silent mating-type cassette damaged DNA binding|RNA binding nuclear chromatin|nuclear telomeric heterochromatin|nuclear envelope|Ku70:Ku80 complex YJR009C TDH2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0221100056392119 0.200357409946563 881 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication gluconeogenesis|reactive oxygen species metabolic process|glycolysis|apoptotic process glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YLL006W-A_p YLL006W-A hom 0.0220919692642914 0.200724875964085 882 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YCR075W-A_p YCR075W-A hom 0.0220718868782417 0.201134604759464 883 Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YNL181W_p YNL181W het FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0220701636693486 0.201304200689932 884 Putative oxidoreductase; required for cell viability biological_process oxidoreductase activity|oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor nuclear envelope|endoplasmic reticulum YOR156C NFI1 hom FT CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.0220330944993961 0.20192778840394 885 SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length protein sumoylation|chromosome segregation|chromosome condensation SUMO ligase activity nuclear chromatin|nucleus|cytoplasm YNR034W SOL1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0220073174273515 0.20245610842988 886 Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress tRNA export from nucleus molecular_function|6-phosphogluconolactonase activity nucleus|cytoplasm YMR129W POM152 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|NUCLEAR PORE 0.022006267981873 0.202477638894227 887 Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 nucleocytoplasmic transport|nuclear pore organization|protein import into nucleus|spindle pole body duplication associated with nuclear envelope protein anchor|structural constituent of nuclear pore mitochondrion|integral to membrane|nuclear pore transmembrane ring|nuclear envelope lumen|nuclear pore YAL053W FLC2 hom FT CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0219449491717141 0.203738554969136 888 Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication protein folding|fungal-type cell wall biogenesis|FAD transport|transmembrane transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|cytoplasm YNR071C_p YNR071C hom FT CARBOHYDRATE METABOLISM 0.0219082652187383 0.204495623361177 889 Putative aldose 1-epimerase biological_process molecular_function cellular_component YNL332W THI12 hom 0.0219073025805029 0.204583019298636 890 Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 thiamine biosynthetic process molecular_function cellular_component YER086W ILV1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0218820986952895 0.205036885732397 891 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation threonine catabolic process|isoleucine biosynthetic process L-threonine ammonia-lyase activity mitochondrion YPR041W TIF5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0218760778862795 0.205161575169667 892 Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 mature ribosome assembly|negative regulation of translational initiation|regulation of translational initiation translation initiation factor binding|GDP-dissociation inhibitor activity|GTPase activator activity|translation initiation factor activity cytosolic small ribosomal subunit|multi-eIF complex YHL023C NPR3 hom FT CELL CYCLE|SIGNALING|RESPONSE TO NUTRIENT LEVELS 0.0218593722492341 0.205507833211799 893 Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair meiosis|regulation of TOR signaling cascade|cellular response to amino acid starvation molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YER175W-A_p YER175W-A hom 0.0218574017438119 0.205548703885734 894 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YJL165C HAL5 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS 0.0218408582639977 0.206569510658883 895 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication cellular cation homeostasis|protein phosphorylation|cellular protein localization|protein dephosphorylation protein kinase activity cellular_component YPR033C HTS1 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0217862717648594 0.207163461519235 896 Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome histidyl-tRNA aminoacylation|mitochondrial translation aminoacyl-tRNA ligase activity mitochondrion|cytoplasm YOL077W-A ATP19 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0217825791442693 0.207104986497216 897 Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YJL183W MNN11 hom FT CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0217605444039681 0.207768327184674 898 Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p protein glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YDR170C SEC7 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.021757878556483 0.207620621490713 899 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|autophagic vacuole assembly ARF guanyl-nucleotide exchange factor activity trans-Golgi network|cytosol|late endosome|integral to membrane|Golgi-associated vesicle YLR222C UTP13 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0217395584145912 0.208003662799984 900 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YIL106W MOB1 het FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.0217175892354958 0.20846367401183 901 Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress regulation of exit from mitosis|protein phosphorylation kinase regulator activity spindle pole body|cellular bud neck YBR137W YBR137W hom 0.0216847056196756 0.209153598520966 902 Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene biological_process molecular_function TRC complex|cytoplasm YDL051W LHP1 hom FT RNA PROCESSING|NUCLEUS 0.0216801044739446 0.209250265931032 903 RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding|tRNA binding nucleoplasm|nucleolus|nucleus YOR386W PHR1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0216742256955685 0.209373822726523 904 DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p photoreactive repair mRNA binding|deoxyribodipyrimidine photo-lyase activity mitochondrion|nucleus YBR066C NRG2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0216374533936336 0.210147880468235 905 Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter by glucose|biofilm formation|pseudohyphal growth|Unknown sequence-specific DNA binding nucleus YPL220W RPL1A hom FT TRANSLATION|RIBOSOME 0.0216313261820615 0.210277059364828 906 Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YLR139C SLS1 hom FT TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0216004821776342 0.210928210344393 907 Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery aerobic respiration|mitochondrial translation molecular_function mitochondrial nucleoid|mitochondrion|integral to membrane|mitochondrial inner membrane YDR374C_p YDR374C hom 0.0215995606380867 0.211492936873454 908 Putative protein of unknown function biological_process molecular_function cellular_component YFL005W SEC4 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|ENDOMEMBRANE SYSTEM|MITOCHONDRION|PLASMA MEMBRANE|CYTOSKELETON 0.0215776199664858 0.211411796686501 909 Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane bipolar cellular bud site selection|autophagy|exocytosis|Golgi to plasma membrane transport|small GTPase mediated signal transduction|ascospore-type prospore assembly|membrane addition at site of cytokinesis|vesicle fusion|cytokinesis GTP binding|GTPase activity mitochondrial outer membrane|vesicle|incipient cellular bud site|mitochondrion|transport vesicle|actin cap|plasma membrane YDL198C GGC1 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0215623812309452 0.212075489048324 910 Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family cellular iron ion homeostasis|mitochondrial genome maintenance|transmembrane transport|guanine nucleotide transport guanine nucleotide transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YPL121C MEI5 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0215380320153699 0.21225106331756 911 Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p meiotic DNA recombinase assembly|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YOR144C ELG1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0215109543766664 0.212826494573929 912 Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication negative regulation of DNA recombination|negative regulation of transposition, RNA-mediated|telomere maintenance|double-strand break repair via homologous recombination|DNA-dependent DNA replication|mitotic sister chromatid cohesion chromatin binding Elg1 RFC-like complex|mitochondrion|nucleus|cytoplasm YOL100W PKH2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0214994353761313 0.213071627781115 913 Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p regulation of nuclear-transcribed mRNA poly(A) tail shortening|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cell cortex|eisosome|nucleus YCR083W TRX3 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0214967210363647 0.213129420671705 914 Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p cellular response to oxidative stress disulfide oxidoreductase activity mitochondrion YLR213C CRR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.0214561228912577 0.213995174014785 915 Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation ascospore wall assembly hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ascospore wall YBR039W ATP3 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0214051042460799 0.215086735595923 916 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis ATP synthesis coupled proton transport proton-transporting ATPase activity, rotational mechanism mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, central stalk YKR088C TVP38 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0213999636023323 0.215196943461789 917 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vesicle-mediated transport|mitotic spindle elongation molecular_function integral to membrane|integral to Golgi membrane|cytoplasm YKL197C PEX1 hom FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION 0.021379504786106 0.215635953510618 918 AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis protein import into peroxisome matrix, receptor recycling ATPase activity|protein heterodimerization activity peroxisomal membrane|peroxisome YPL119C DBP1 hom FT TRANSLATION 0.0213601642219935 0.216051560393624 919 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress translational initiation RNA helicase activity cytoplasm YBR046C ZTA1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.0213332032020851 0.216631883769573 920 NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin cellular response to oxidative stress NADPH:quinone reductase activity|AU-rich element binding|2-alkenal reductase [NAD(P)] activity nucleus|cytoplasm YKR097W PCK1 hom FT CARBOHYDRATE METABOLISM 0.0213138585285111 0.217048959161491 921 Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) activity cytosol YDR034W-B_p YDR034W-B hom 0.0213124119699774 0.217080170466497 922 Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YML009W-B_d YML009W-B hom 0.0213011016430496 0.217324316144673 923 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YLR016C PML1 hom FT RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.021298244963749 0.217454598053136 924 Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p mRNA export from nucleus|mRNA splicing, via spliceosome|maintenance of RNA location molecular_function RES complex|nucleus|cytoplasm YIR016W_p YIR016W hom 0.0212943843372007 0.217469409878078 925 Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication biological_process molecular_function cellular_component YEL018W EAF5 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0212873879259112 0.217826496820401 926 Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex DNA repair molecular_function nucleus|NuA4 histone acetyltransferase complex YLR080W EMP46 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0212828506188073 0.217718700463177 927 Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport ER to Golgi vesicle-mediated transport glycoprotein binding ER to Golgi transport vesicle|Golgi membrane YER187W_p YER187W hom 0.0212704649482755 0.217986633807013 928 Putative protein of unknown function; induced in respiratory-deficient cells biological_process molecular_function cellular_component YGR132C PHB1 hom FT MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0212510869031221 0.218887501153021 929 Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation mitochondrion inheritance|protein folding|negative regulation of proteolysis|replicative cell aging|mitochondrion morphogenesis|inner mitochondrial membrane organization molecular_function mitochondrion|mitochondrial inner membrane YFR027W ECO1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0212392367627819 0.218663229790483 930 Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress double-strand break repair|regulation of DNA replication|mitotic chromosome condensation|internal peptidyl-lysine acetylation|establishment of mitotic sister chromatid cohesion|DNA repair|chromosome organization|DNA replication|regulation of mitosis|telomere organization|tRNA gene clustering acetyltransferase activity|chromatin binding nuclear chromatin|nuclear replication fork YPR039W_d YPR039W hom 0.0212373871698775 0.218703350680738 931 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Unknown Unknown Unknown YER136W GDI1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT 0.0212366872204925 0.218718535180058 932 GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins vesicle-mediated transport Rab GDP-dissociation inhibitor activity cellular_component YBR221W-A_p YBR221W-A hom 0.0212211723383586 0.219124019481723 933 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YER127W LCP5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0211949514038669 0.219625308530022 934 Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus rRNA modification|rRNA processing RNA binding small nuclear ribonucleoprotein complex|small nucleolar ribonucleoprotein complex YKL169C_d YKL169C hom 0.021181239345471 0.220612772260185 935 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Unknown Unknown Unknown YCR003W MRPL32 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0211649651960711 0.220278466878477 936 Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial inner membrane|mitochondrial large ribosomal subunit YOR194C TOA1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0211527415591378 0.220545120844232 937 TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription factor TFIIA complex YMR135C GID8 hom FT KETONE METABOLISM|CELL CYCLE|CARBOHYDRATE METABOLISM|PROTEOLYSIS|NUCLEUS 0.0210987685431347 0.221725288020657 938 Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|traversing start control point of mitotic cell cycle molecular_function nucleus|GID complex|cytoplasm YCL057W PRD1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0210833396066125 0.222063484861422 939 Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress proteolysis metalloendopeptidase activity mitochondrion|cytoplasm|mitochondrial intermembrane space YER033C ZRG8 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.021080362389175 0.222128786898789 940 Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency fungal-type cell wall organization molecular_function mitochondrion|cellular bud|cellular bud neck|mating projection tip|cytoplasm YNL050C_p YNL050C hom 0.0210105871236504 0.223663169753665 941 Putative protein of unknown function; YNL050c is not an essential gene biological_process molecular_function cellular_component YBR201W DER1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|UBIQUITIN LIGASE COMPLEX 0.0210095738550963 0.223685507564284 942 Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p ER-associated protein catabolic process molecular_function integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YJR015W_p YJR015W hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0210070236626046 0.223741734383979 943 Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum|cytoplasm YBR172C SMY2 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0209930936393294 0.224049042813264 944 Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum membrane|cytoplasmic mRNA processing body|cytoplasm|extrinsic to membrane YER115C SPR6 hom FT CELL CYCLE 0.0209797960017933 0.224342681556953 945 Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation ascospore formation molecular_function cellular_component YOR385W_p YOR385W hom 0.0209779687153722 0.224383053215381 946 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm YGL008C PMA1 het FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.0209551966877753 0.224886608742904 947 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] proton transport|regulation of pH|transmembrane transport hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|membrane raft|plasma membrane YER022W SRB4 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0209506644858387 0.224986925074878 948 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination transcription initiation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter activating transcription factor binding|structural molecule activity|core RNA polymerase II recruiting transcription factor activity core mediator complex YPR161C SGV1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0208822318058126 0.226505507574895 949 Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins phosphorylation of RNA polymerase II C-terminal domain|protein phosphorylation|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YGR024C THG1 het FT RNA PROCESSING|NUCLEUS 0.0208681100459875 0.226819789716589 950 tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes tRNA modification tRNA guanylyltransferase activity nucleus|cytoplasm YPL118W MRP51 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0208471366383026 0.227356438332266 951 Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences mitochondrial translational initiation|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YLR324W PEX30 hom FF|FT PEROXISOME ORGANIZATION 0.0208453897741141 0.227326085384258 952 Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function integral to membrane|peroxisome|integral to peroxisomal membrane YML011C RAD33 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0208239399323351 0.227804809459164 953 Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus nucleotide-excision repair molecular_function nucleus YJR097W JJJ3 hom FT NUCLEUS 0.0208172063264297 0.227955240099581 954 Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 peptidyl-diphthamide biosynthetic process from peptidyl-histidine ferrous iron binding nucleus|cytoplasm YHR036W BRL1 het FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0208120341753952 0.22807083542965 955 Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export mRNA export from nucleus|protein export from nucleus|nuclear envelope organization molecular_function nuclear envelope|endoplasmic reticulum YHR078W_p YHR078W hom 0.0207901122146179 0.228561245108672 956 High osmolarity-regulated gene of unknown function biological_process molecular_function integral to membrane YOL050C_d YOL050C hom 0.0207675319031985 0.229067166786019 957 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Unknown Unknown Unknown YHR087W RTC3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0207628214482126 0.22924224234151 958 Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress RNA metabolic process molecular_function nucleus|cytoplasm YBR255W MTC4 hom 0.020747987194908 0.229505717294935 959 Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm YDL039C PRM7 hom 0.0207450531573942 0.229571603693154 960 Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements conjugation with cellular fusion molecular_function integral to membrane YER053C PIC2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0207298031065461 0.229914273671263 961 Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature phosphate ion transport|transmembrane transport inorganic phosphate transmembrane transporter activity mitochondrion|integral to membrane YDR524C AGE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS 0.0207258544602761 0.230281172281655 962 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport phospholipid binding|ARF GTPase activator activity endosome|trans-Golgi network|cytoplasm YDR020C_p DAS2 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0207188791516079 0.230159959318279 963 Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases biological_process molecular_function nucleus|cytoplasm YER093C TSC11 het FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.0207165882256775 0.23021150714121 964 Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|sphingolipid biosynthetic process protein binding TORC2 complex YOL024W_p YOL024W hom 0.0206834598190719 0.23095784243604 965 Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication biological_process molecular_function cellular_component YGL099W LSG1 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|SITE OF POLARIZED GROWTH 0.0206797676051778 0.231041129015477 966 Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm conjugation with cellular fusion|ribosomal large subunit assembly|ribosomal subunit export from nucleus|ascospore formation GTPase activity mating projection tip|cytoplasm YGR175C ERG1 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0206484695941098 0.231747986194122 967 Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine ergosterol biosynthetic process drug binding|squalene monooxygenase activity endoplasmic reticulum|lipid particle YHR038W RRF1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0206072366493039 0.232681562339212 968 Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria mitochondrial translation translation release factor activity mitochondrion YHR019C DED81 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0205993406132707 0.233069742824971 969 Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity cytosol YML034C-A_d YML034C-A hom 0.0205617001084384 0.233715673979554 970 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR083W TEC1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0205561090609076 0.233842867917645 971 Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member pseudohyphal growth|chronological cell aging|positive regulation of transposition, RNA-mediated|invasive growth in response to glucose limitation|positive regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YNL092W_p YNL092W hom 0.0205433181819393 0.234134039342034 972 Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YPR108W-A_p YPR108W-A hom 0.0205432335617643 0.234345303976074 973 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGR218W CRM1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0205224645718892 0.234818726174509 974 Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin mRNA export from nucleus|protein export from nucleus|protein localization to kinetochore|ribosomal large subunit export from nucleus protein transporter activity integral to membrane|nucleus|spindle pole body|kinetochore YPL038W-A_p YPL038W-A hom 0.020504506700993 0.235019114806937 975 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGR082W TOM20 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION|MITOCHONDRION 0.0205013885163947 0.235090325989594 976 Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins tRNA import into mitochondrion|protein import into mitochondrial matrix|mitochondrial outer membrane translocase complex assembly protein channel activity|mitochondrion targeting sequence binding mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YPL048W CAM1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS 0.0204932218324159 0.235276904055568 977 Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma ribosome biogenesis|positive regulation of transcription from RNA polymerase II promoter phospholipid binding|transcription coactivator activity|calcium ion binding nucleus YMR227C TAF7 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0204240318810388 0.236861839204281 978 TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YOR306C MCH5 hom FT PLASMA MEMBRANE 0.020412936332175 0.237186639944057 979 Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport riboflavin transport monocarboxylic acid transmembrane transporter activity|riboflavin transporter activity integral to membrane|plasma membrane YER147C SCC4 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0204125379727619 0.237125859245944 980 Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX double-strand break repair|establishment of protein localization to chromatin|rDNA condensation|mitotic chromosome condensation|mitotic sister chromatid cohesion molecular_function nuclear chromatin|SMC loading complex YOR177C MPC54 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|NUCLEUS|CYTOSKELETON 0.0204052203981789 0.237294055357944 981 Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate ascospore wall assembly|vesicle docking structural molecule activity spindle pole body YGR155W CYS4 hom FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|MITOCHONDRION 0.020400048293191 0.238043458698412 982 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria cysteine biosynthetic process from serine|hydrogen sulfide biosynthetic process|cysteine biosynthetic process via cystathionine|transsulfuration|traversing start control point of mitotic cell cycle cystathionine beta-synthase activity mitochondrion|cytoplasm YNR019W ARE2 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0203497791460231 0.23885135232496 983 Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication ergosterol metabolic process ergosterol O-acyltransferase activity integral to membrane|endoplasmic reticulum YDL098C SNU23 het FT RNA PROCESSING|NUCLEUS 0.0203486674225939 0.238877015871825 984 Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YNL042W BOP3 hom FT NUCLEUS 0.0203438610015554 0.238987991935387 985 Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury cellular response to methylmercury molecular_function nucleus|cytoplasm YKR100C SKG1 hom FT CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0203388709316771 0.23889299357492 986 Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell fungal-type cell wall organization molecular_function incipient cellular bud site|cellular bud|cellular bud tip YLR196W PWP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0203224120377761 0.239203297690294 987 Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily rRNA processing molecular_function nucleolus|nucleus|cytoplasm YBR139W YBR139W hom FT PROTEOLYSIS 0.0202703884675942 0.240408286371493 988 Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner phytochelatin biosynthetic process serine hydrolase activity|serine-type carboxypeptidase activity fungal-type vacuole YHR121W LSM12 hom FT NUCLEUS 0.0202684619277642 0.240452991522211 989 Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress RNA metabolic process RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YNL031C HHT2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0202285371077379 0.241380760762837 990 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation chromatin assembly or disassembly|global genome nucleotide-excision repair|rRNA transcription|sexual sporulation resulting in formation of a cellular spore|mitotic cell cycle spindle assembly checkpoint DNA binding nuclear nucleosome YGR262C BUD32 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|LIPID METABOLISM|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|NUCLEUS|CHROMOSOME 0.0202173495037036 0.241641188481163 991 Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription threonylcarbamoyladenosine metabolic process|protein phosphorylation|telomere maintenance|cellular bud site selection|positive regulation of transcription from RNA polymerase II promoter protein serine/threonine kinase activity EKC/KEOPS complex|nucleus|cytoplasm YGR001C_p YGR001C hom 0.0202077425546095 0.242216258019361 992 Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae biological_process methyltransferase activity cytoplasm YML130C ERO1 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0201759056265282 0.242607651122568 993 Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds protein folding|protein thiol-disulfide exchange thiol oxidase activity endoplasmic reticulum YOR232W MGE1 het FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0201673545022247 0.243018246637743 994 Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE protein refolding|protein import into mitochondrial matrix adenyl-nucleotide exchange factor activity mitochondrion|presequence translocase-associated import motor|mitochondrial matrix YHR010W RPL27A hom FT TRANSLATION|RIBOSOME 0.0201667031449582 0.242822619206285 995 Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YPL025C_d YPL025C hom 0.0201233529090281 0.243837072218002 996 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR289W SNF5 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.0200849330145997 0.244738632771568 997 Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|double-strand break repair via homologous recombination|chromatin remodeling|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding SWI/SNF complex|nucleus YBR267W REI1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|CELL DIVISION|RIBOSOME 0.0200727188431423 0.245025739583461 998 Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network ribosomal large subunit biogenesis|nucleocytoplasmic transport|mitotic cell cycle|budding cell bud growth sequence-specific DNA binding cytosolic large ribosomal subunit|preribosome, large subunit precursor|cytoplasm YLR279W_d YLR279W hom 0.0200628223922772 0.245258539136732 999 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL072C PSF2 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0200556262358085 0.245498312560428 1000 Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|nuclear replication fork|nucleus|DNA replication preinitiation complex YGR110W CLD1 hom FT LIPID METABOLISM|MITOCHONDRION 0.0200500272432542 0.245700588505528 1001 Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 cardiolipin acyl-chain remodeling|cardiolipin metabolic process phospholipase A2 activity mitochondrion YBR160W CDC28 het FT CELL CYCLE|LIPID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0200408210668751 0.245776643740816 1002 Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress regulation of budding cell apical bud growth|negative regulation of meiotic cell cycle|positive regulation of triglyceride catabolic process|protein phosphorylation|regulation of filamentous growth|negative regulation of transcription, DNA-dependent|positive regulation of meiotic cell cycle|positive regulation of transcription, DNA-dependent|regulation of double-strand break repair via homologous recombination|vesicle-mediated transport|positive regulation of DNA replication involved in S phase|positive regulation of mitotic cell cycle|positive regulation of spindle pole body separation|chromatin remodeling|negative regulation of mitotic cell cycle histone binding|protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|ribosome|endoplasmic reticulum|nucleus|spindle pole body|cellular bud neck|astral microtubule|cytoplasm YDR225W HTA1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|RSC COMPLEX 0.0200345571639923 0.245924291266256 1003 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair|chromatin assembly or disassembly|negative regulation of transcription from RNA polymerase II promoter DNA binding replication fork protection complex|nuclear nucleosome YLR216C CPR6 hom 0.020027166221636 0.246098584358117 1004 Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding|protein refolding unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytoplasm YHR170W NMD3 het FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS|RIBOSOME 0.0200234777438994 0.247032460755648 1005 Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex ribosomal large subunit export from nucleus ribosomal large subunit binding cytosol|cytosolic large ribosomal subunit YJR083C ACF4 hom 0.0200217984883845 0.246225220406653 1006 Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate biological_process molecular_function cellular_component YHR111W UBA4 hom FT RNA PROCESSING|CELL DIVISION|RESPONSE TO OXIDATIVE STRESS 0.0200067193219827 0.24679265688042 1007 Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p cellular response to oxidative stress|tRNA wobble position uridine thiolation|cell budding|protein urmylation|tRNA thio-modification|Unknown|invasive growth in response to glucose limitation URM1 activating enzyme activity|sulfurtransferase activity|thiosulfate sulfurtransferase activity|protein adenylyltransferase activity cytoplasm YBL078C ATG8 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|ENDOMEMBRANE SYSTEM 0.019962505382572 0.247627106632345 1008 Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis piecemeal microautophagy of nucleus|mitochondrion degradation|cellular membrane fusion|ER to Golgi vesicle-mediated transport|CVT pathway|autophagic vacuole assembly molecular_function cytosol|fungal-type vacuole membrane|pre-autophagosomal structure|extrinsic to membrane|autophagic vacuole YGL119W COQ8 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0199498605681609 0.248279660758088 1009 Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication ubiquinone biosynthetic process|aerobic respiration molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial matrix YGR075C PRP38 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.019942813769217 0.248164447559891 1010 Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) molecular_function U4/U6 x U5 tri-snRNP complex YCR061W_p YCR061W hom 0.0199188214376604 0.248663507000251 1011 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation regulation of cell size molecular_function integral to membrane|cytoplasm YOR228C YOR228C hom FT MITOCHONDRION 0.0198999579498705 0.249111978817074 1012 Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to membrane YCL032W STE50 hom FT CELL CYCLE|SIGNALING 0.0198786689887858 0.249972076957811 1013 Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction pheromone-dependent signal transduction involved in conjugation with cellular fusion|MAPK cascade involved in osmosensory signaling pathway|osmosensory signaling pathway via Sho1 osmosensor|signal transduction involved in filamentous growth|cellular response to heat SAM domain binding|protein kinase regulator activity cytoplasm YNL254C RTC4 hom FT NUCLEUS 0.0198722924364606 0.249770737049253 1014 Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YJL069C UTP18 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.019867400729485 0.249887342536678 1015 Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YML122C_d YML122C hom FF 0.019847881072901 0.251202351465919 1016 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR457W TOM1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.0198463265387664 0.250390130687895 1017 E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mitosis|nucleocytoplasmic transport|nucleus organization|cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of cell size|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|nucleolus|nucleus YBR053C_p YBR053C hom 0.0198363454309065 0.250699162427134 1018 Putative protein of unknown function; induced by cell wall perturbation biological_process molecular_function cellular_component YNL316C PHA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0198164424201926 0.25110431601671 1019 Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway L-phenylalanine biosynthetic process prephenate dehydratase activity cytoplasm YMR134W ERG29 het FT LIPID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0197809738633657 0.252307640513606 1020 Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes cellular iron ion homeostasis|mitochondrion organization molecular_function nuclear envelope|endoplasmic reticulum|cytoplasm YGR090W UTP22 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0197621948481554 0.25261667077386 1021 Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|UTP-C complex|90S preribosome|CURI complex YJL064W_d YJL064W hom 0.0197583937613288 0.252495649045647 1022 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Unknown Unknown Unknown YLR170C APS1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0197161134362322 0.253512414879012 1023 Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YOR027W STI1 hom 0.0197041646976508 0.253800276319449 1024 Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop protein folding Hsp70 protein binding|Hsp90 protein binding|mRNA binding|ATPase inhibitor activity cytoplasm YNL049C SFB2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0197003337883298 0.254175991562398 1025 Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat YIL119C RPI1 hom FT SIGNALING|NUCLEUS 0.0196981970805055 0.253944129498968 1026 Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF fungal-type cell wall biogenesis|Ras protein signal transduction|positive regulation of transcription from RNA polymerase II promoter small GTPase regulator activity nucleus YDR215C_d YDR215C hom 0.0196908210374876 0.254405428439804 1027 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Unknown Unknown Unknown YER079W_p YER079W hom FT NUCLEUS 0.0196604508278175 0.254855340878141 1028 Putative protein of unknown function biological_process molecular_function nucleus|cytoplasm YDR275W BSC2 hom 0.019652520763598 0.255047064718063 1029 Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function lipid particle YLR257W YLR257W hom 0.0196513989528609 0.255074194640402 1030 Protein of unknown function; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm YGR113W DAM1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0196245382477943 0.255937131941604 1031 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle midzone|DASH complex|condensed nuclear chromosome kinetochore|kinetochore microtubule|spindle YBR103W SIF2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|GENE SILENCING|NUCLEUS|CHROMOSOME 0.019623479735122 0.255750040463075 1032 WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus negative regulation of meiosis|negative regulation of chromatin silencing at telomere|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|nucleus|Set3 complex YGR052W_p FMP48 hom FT PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.0195987259252556 0.25635030005781 1033 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation protein phosphorylation protein kinase activity mitochondrion YMR061W RNA14 het FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0195906330524699 0.256546757559592 1034 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation RNA binding|protein heterodimerization activity mRNA cleavage factor complex|mitochondrion|nucleus YKL126W YPK1 hom FF|FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.019576735823428 0.257097248999548 1035 Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication eisosome assembly|protein autophosphorylation|negative regulation of phospholipid translocation|protein phosphorylation protein serine/threonine kinase activity cytosol|plasma membrane|cellular bud neck YPL271W ATP15 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|MITOCHONDRION 0.0195060530906444 0.259816682910275 1036 Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, central stalk YGL006W-A_p YGL006W-A hom 0.0194534254818736 0.259893432988041 1037 Putative protein of unknown function; identified by SAGE biological_process molecular_function cellular_component YHR107C CDC12 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0194130546042179 0.261595960434821 1038 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells maintenance of cell polarity|cell cycle cytokinesis|positive regulation of protein kinase activity|septin ring assembly protein complex scaffold|structural molecule activity|GTP binding|GTPase activity|1-phosphatidylinositol binding septin filament array|cellular bud neck septin ring|septin complex|cytoplasm|mating projection YLR307C-A_p YLR307C-A hom 0.019401056657896 0.261534516719706 1039 Putative protein of unknown function biological_process molecular_function cellular_component YPL245W_p YPL245W hom FT NUCLEUS 0.0193652858013254 0.262059145841314 1040 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm biological_process molecular_function nucleus|cytoplasm YML107C PML39 hom FT RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0193359054168537 0.262783823973638 1041 Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes maintenance of RNA location molecular_function ribosome|nuclear pore YLR135W SLX4 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0193356026229659 0.262791299690636 1042 Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress double-strand break repair via single-strand annealing, removal of nonhomologous ends|DNA replication|response to DNA damage stimulus|DNA-dependent DNA replication 5'-flap endonuclease activity Slx1-Slx4 complex|nucleus YDL220C CDC13 het FT CELL CYCLE|CELL DIVISION|NUCLEUS|CHROMOSOME 0.019301914529544 0.26362394599019 1043 Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation telomere capping|telomere maintenance via telomerase|negative regulation of telomerase activity|telomere maintenance|regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding|telomerase inhibitor activity nuclear telomere cap complex YHR045W_p YHR045W hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.0192876790332482 0.263976341561539 1044 Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum biological_process molecular_function endoplasmic reticulum YMR304W UBP15 hom FT PROTEOLYSIS 0.0192688252030807 0.2647286537817 1045 Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p protein deubiquitination ubiquitin-specific protease activity peroxisome|cytoplasm YLR220W CCC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0192509340847456 0.264887452508097 1046 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress manganese ion transport|cellular calcium ion homeostasis|cellular iron ion homeostasis|cellular manganese ion homeostasis|ferrous iron transport manganese ion transmembrane transporter activity|ferrous iron transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|Golgi apparatus YNL200C YNL200C hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0192313420506941 0.265374131964905 1047 NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies nicotinamide nucleotide metabolic process NADHX epimerase activity mitochondrion|cytoplasm YPR007C REC8 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0191502100601853 0.267396056957198 1048 Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p synaptonemal complex assembly|protein localization to chromosome, centromeric region|reciprocal meiotic recombination|meiotic sister chromatid cohesion molecular_function condensed nuclear chromosome|condensed nuclear chromosome, centromeric region YDR241W_d BUD26 hom 0.0191475133601091 0.267463443872797 1049 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YBL030C PET9 het FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0191112234753005 0.26858560982894 1050 Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication heme transport|transmembrane transport|aerobic respiration|anaerobic respiration|ATP transport|ADP transport|apoptotic process|mitochondrial transport ATP:ADP antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YGL223C COG1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0190882224622332 0.269448247823445 1051 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YHR130C_d YHR130C hom 0.0190756515486319 0.269263473812906 1052 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL126W NAN1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0190542358597926 0.26980150817667 1053 U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|RENT complex|90S preribosome YMR047C NUP116 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0190133359773669 0.270831098372516 1054 FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) poly(A)+ mRNA export from nucleus|mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YOL023W IFM1 hom FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0189773946653581 0.271738083464484 1055 Mitochondrial translation initiation factor 2 translational initiation|mitochondrial translational initiation|mitochondrial translation tRNA binding|GTPase activity|translation initiation factor activity mitochondrion YNL071W LAT1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0189571757228684 0.272249224610032 1056 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA acetyl-CoA biosynthetic process from pyruvate dihydrolipoyllysine-residue acetyltransferase activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YCR054C CTR86 het FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0189446779651334 0.272637009233249 1057 Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function cytoplasm YGL125W MET13 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0189336776686312 0.272844088666657 1058 Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway methionine biosynthetic process methylenetetrahydrofolate reductase (NADPH) activity mitochondrion YDR114C_p YDR114C hom 0.0189283575220915 0.27297889391718 1059 Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium biological_process molecular_function cellular_component YKL124W SSH4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0189070632475814 0.273518917593953 1060 Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|vesicle-mediated transport molecular_function fungal-type vacuole YDL141W BPL1 het FT NUCLEUS 0.0188946309450767 0.273834538589096 1061 Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation protein biotinylation biotin-[pyruvate-carboxylase] ligase activity|biotin-[acetyl-CoA-carboxylase] ligase activity nucleus|cytoplasm YLR304C ACO1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0188755762847229 0.274318765237746 1062 Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy propionate metabolic process|glutamate biosynthetic process|tricarboxylic acid cycle|mitochondrial genome maintenance|citrate metabolic process double-stranded DNA binding|aconitate hydratase activity|single-stranded DNA binding mitochondrial nucleoid|cytosol|mitochondrion|mitochondrial matrix YJL028W YJL028W hom 0.0188664144974045 0.274551797222931 1063 Protein of unknown function; may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome YCL044C MGR1 hom FT PROTEOLYSIS|MITOCHONDRION 0.0188467572142557 0.275625551011096 1064 Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA misfolded or incompletely synthesized protein catabolic process misfolded protein binding mitochondrion|i-AAA complex YCR075C ERS1 hom 0.0188079707469326 0.276041506621495 1065 Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains L-cystine transport|transmembrane transport L-cystine transmembrane transporter activity endosome|integral to membrane|fungal-type vacuole YER133W GLC7 het FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CELL DIVISION|CARBOHYDRATE METABOLISM 0.0188031543096762 0.276379430587575 1066 Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest replication fork processing|glycogen metabolic process|chromosome segregation|regulation of cell shape during vegetative growth phase|termination of RNA polymerase II transcription, poly(A)-coupled|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|rRNA processing|regulation of cell cycle|ascospore formation|protein localization to kinetochore|cell budding|DNA damage checkpoint|meiosis|termination of RNA polymerase II transcription, exosome-dependent|DNA replication checkpoint|response to heat|cellular ion homeostasis|histone dephosphorylation|regulation of carbohydrate metabolic process protein serine/threonine phosphatase activity protein phosphatase type 1 complex|mating projection base|condensed nuclear chromosome kinetochore|mRNA cleavage and polyadenylation specificity factor complex|nucleolus|spindle pole body|cellular bud neck YHR108W GGA2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.0187691278119424 0.27703464132764 1067 Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to vacuole transport|Golgi to endosome transport ubiquitin binding|phosphatidylinositol-4-phosphate binding trans-Golgi network YKL187C FAT3 hom FT MITOCHONDRION 0.0187640002406384 0.27773970155979 1068 Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|integral to membrane YGR268C HUA1 hom 0.018750764132739 0.277505009394185 1069 Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YBR291C CTP1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0187019612863042 0.278757688873554 1070 Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family mitochondrial citrate transport|transmembrane transport tricarboxylate secondary active transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YGR030C POP6 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0186837506181199 0.279226106899412 1071 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YDL211C_p YDL211C hom 0.0186707237068353 0.279776694014018 1072 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YPR094W RDS3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0186665133107809 0.279669980577278 1073 Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance spliceosomal complex assembly|response to xenobiotic stimulus|mRNA splicing, via spliceosome molecular_function U2 snRNP YER039C-A_p YER039C-A hom 0.018658760308727 0.279869782455493 1074 Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function integral to membrane YJR110W YMR1 hom FT LIPID METABOLISM 0.0186492975834226 0.280113776816084 1075 Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family phosphatidylinositol dephosphorylation mRNA binding|phosphatidylinositol-3-phosphatase activity cytoplasm YDL053C PBP4 hom FT NUCLEUS 0.0186365616455801 0.280442398269741 1076 Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasmic stress granule|cytoplasm YPL022W RAD1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS 0.018570396520526 0.2821538476859 1077 Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein mitotic recombination|meiotic mismatch repair|nucleotide-excision repair, DNA incision, 5'-to lesion|double-strand break repair via single-strand annealing, removal of nonhomologous ends|removal of nonhomologous ends single-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleotide-excision repair factor 1 complex|nucleus YMR309C NIP1 het FT TRANSLATION 0.0185619390083139 0.282588504013911 1078 eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YHR025W THR1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0185410617938192 0.282914890162709 1079 Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway threonine biosynthetic process|methionine metabolic process|isoleucine metabolic process|homoserine metabolic process homoserine kinase activity cellular_component YDR122W KIN1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.0185159664292682 0.283567051757266 1080 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p protein autophosphorylation|exocytosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity internal side of plasma membrane|plasma membrane YPL183W-A RTC6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0184976504350146 0.284043677539928 1081 Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress mitochondrial translation|ribosome biogenesis structural constituent of ribosome mitochondrial large ribosomal subunit YIL120W QDR1 hom FT PLASMA MEMBRANE 0.0184873391224435 0.284312240796942 1082 Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication drug transmembrane transport drug transmembrane transporter activity integral to membrane|plasma membrane YDR408C ADE8 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0184836149774103 0.284696685058274 1083 Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process|adenine biosynthetic process phosphoribosylglycinamide formyltransferase activity nucleus|cytoplasm YPL133C RDS2 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0184554880712864 0.285142901772699 1084 Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|cellular response to xenobiotic stimulus|positive regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YDR158W HOM2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0184206141514059 0.286918010087025 1085 Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis methionine biosynthetic process|threonine biosynthetic process|homoserine biosynthetic process aspartate-semialdehyde dehydrogenase activity nucleus|plasma membrane|cytoplasm YNL163C RIA1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM 0.0184115772080244 0.286362620811896 1086 Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes mature ribosome assembly GTPase activity cytoplasm YOR346W REV1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0184097474418346 0.286338663524439 1087 Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress error-prone translesion synthesis|error-free translesion synthesis deoxycytidyl transferase activity|DNA-directed DNA polymerase activity nuclear chromatin|mitochondrion|replication fork YDR223W CRF1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0184068573573447 0.286414330312083 1088 Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter transcription corepressor activity nuclear chromatin|nucleus|cytoplasm YJR082C EAF6 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0183680621578511 0.287431354244075 1089 Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 DNA repair|histone acetylation histone acetyltransferase activity nucleus|NuA4 histone acetyltransferase complex|NuA3 histone acetyltransferase complex YJR061W_p YJR061W hom 0.0183621433139973 0.287658614027068 1090 Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication biological_process molecular_function cellular_component YCR098C GIT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0183410760324373 0.288140235717033 1091 Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability transmembrane transport|glycerophosphodiester transport glycerophosphodiester transmembrane transporter activity integral to membrane|plasma membrane YCR076C FUB1 hom 0.0183360032561774 0.288273620735732 1092 Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene biological_process proteasome binding cellular_component YKR044W UIP5 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.0183257696426376 0.288542832911618 1093 Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates biological_process molecular_function nuclear envelope YGR253C PUP2 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0183243141904992 0.288940869093863 1094 Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YDR022C ATG31 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|CHROMOSOME SEGREGATION|CYTOSKELETON 0.0183164882192853 0.288787142588059 1095 Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion autophagy|mitochondrion degradation|piecemeal microautophagy of nucleus molecular_function pre-autophagosomal structure|cytoplasm YIL057C RGI2 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0183127594584922 0.288885331881326 1096 Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose energy reserve metabolic process molecular_function cytoplasm YLR342W-A_p YLR342W-A hom 0.0183109651594622 0.288932589091438 1097 Putative protein of unknown function biological_process molecular_function cellular_component YML015C TAF11 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0183090675533048 0.289054481546837 1098 TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly transcription factor TFIID complex YNL011C_p YNL011C hom 0.0183077527046524 0.289017209873648 1099 Putative protein of unknown function; YNL011C is not an essential gene biological_process molecular_function cellular_component YGR134W CAF130 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0182884849875149 0.289525100121737 1100 Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex YDL003W MCD1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.018284805555266 0.289622156941025 1101 Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase double-strand break repair|apoptotic process|response to DNA damage stimulus|mitotic chromosome condensation|establishment of mitotic sister chromatid cohesion chromatin binding condensed nuclear chromosome|mitochondrion|nucleus|nuclear mitotic cohesin complex YJL067W_d YJL067W hom 0.018237188172156 0.291168100418555 1102 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR193W MRPL24 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0182054879715303 0.29179169412126 1103 Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YIL098C FMC1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0182046133537202 0.291742923594286 1104 Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p mitochondrial proton-transporting ATP synthase complex assembly molecular_function mitochondrion|mitochondrial matrix YKL043W PHD1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0181604781919133 0.292914563705182 1105 Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication pseudohyphal growth|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YBL018C POP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0181250911536126 0.294216427830303 1106 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YHR005C-A TIM10 het FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0181172844709305 0.294064264647522 1107 Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity|unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex|mitochondrial intermembrane space protein transporter complex YJR091C JSN1 hom FT PROTEIN LOCALIZATION|MITOCHONDRION 0.0180692133750001 0.295563445386461 1108 Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|cellular protein complex localization|establishment of mitochondrion localization mRNA binding external side of mitochondrial outer membrane YBR161W CSH1 hom FT LIPID METABOLISM 0.0179897443862089 0.297476670360049 1109 Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication glycosphingolipid biosynthetic process|sphingolipid biosynthetic process mannosyltransferase activity|transferase activity, transferring glycosyl groups integral to membrane|fungal-type vacuole YLL020C_d YLL020C hom 0.01797030700422 0.297999042165714 1110 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Unknown Unknown Unknown YFR040W SAP155 hom FT CELL CYCLE 0.0179696930402047 0.298015552174462 1111 Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity cytoplasm YGR193C PDX1 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0179683836991396 0.298122811955548 1112 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core acetyl-CoA biosynthetic process from pyruvate structural molecule activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YMR115W MGR3 hom FT PROTEOLYSIS|MITOCHONDRION 0.0179680505500141 0.298059723119702 1113 Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA misfolded or incompletely synthesized protein catabolic process misfolded protein binding mitochondrion|mitochondrial inner membrane|i-AAA complex YAL044C GCV3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0179625018135287 0.298208975781445 1114 H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF glycine catabolic process|one-carbon metabolic process|protein lipoylation glycine dehydrogenase (decarboxylating) activity mitochondrion|glycine cleavage complex YIL002C INP51 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|CYTOSKELETON 0.0179500834910196 0.298615245192446 1115 Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth phosphatidylinositol dephosphorylation phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity membrane|cytoplasm YPR106W ISR1 hom FT PROTEIN PHOSPHORYLATION 0.017940568651216 0.298799433202823 1116 Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C biological_process protein kinase activity cellular_component YKR069W MET1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.0179308972665079 0.29906004189855 1117 S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis siroheme biosynthetic process|methionine biosynthetic process|sulfate assimilation uroporphyrin-III C-methyltransferase activity intracellular YHR039C MSC7 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0179238915310529 0.299681627398841 1118 Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids reciprocal meiotic recombination molecular_function integral to membrane|endoplasmic reticulum YIL011W TIR3 hom 0.0179044677277981 0.299989268209184 1119 Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth biological_process molecular_function fungal-type cell wall YLR452C SST2 hom FT SIGNALING|PLASMA MEMBRANE 0.0178987042310139 0.299928619396152 1120 GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|signal transduction GTPase activator activity plasma membrane YNL180C RHO5 hom FT SIGNALING|NUCLEUS|PLASMA MEMBRANE 0.0178763871653027 0.300748024068683 1121 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Rho protein signal transduction GTPase activity endomembrane system|fungal-type vacuole membrane|plasma membrane|nucleus|cytoplasm YAL064C-A_p TDA8 hom 0.0178657830024737 0.30081858096945 1122 Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene biological_process molecular_function cellular_component YOL036W_p YOL036W hom 0.0178487493125145 0.301279743407924 1123 Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication biological_process molecular_function cellular_component YLR255C_d YLR255C hom 0.0178395856774587 0.301528030113947 1124 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL035W UGP1 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0178298382055402 0.301792285174016 1125 UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication (1->6)-beta-D-glucan biosynthetic process|UDP-glucose metabolic process|trehalose biosynthetic process|glycogen biosynthetic process UTP:glucose-1-phosphate uridylyltransferase activity plasma membrane|cytoplasm YGR109C CLB6 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0178266074628773 0.302240581312921 1126 B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 regulation of cyclin-dependent protein kinase activity|G1/S transition of mitotic cell cycle|regulation of S phase of mitotic cell cycle|premeiotic DNA replication cyclin-dependent protein kinase regulator activity cellular_component YAL009W SPO7 hom FF|FT CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0177621712907214 0.303703096463758 1127 Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation negative regulation of phospholipid biosynthetic process|nuclear envelope organization phosphoprotein phosphatase activity Nem1-Spo7 phosphatase complex|integral to membrane YBR210W ERV15 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION 0.0177556337396718 0.303881135330751 1128 Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle YMR240C CUS1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0177467021807752 0.304052372276133 1129 Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p spliceosomal complex assembly|mRNA splicing, via spliceosome molecular_function U2 snRNP|U2-type prespliceosome YDR246W-A_p YDR246W-A hom 0.0177247069193693 0.304652197397738 1130 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR099W KTR1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0177191288717957 0.304804439111595 1131 Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress N-glycan processing|cell wall mannoprotein biosynthetic process|protein O-linked glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YPL236C ENV7 hom FT PROTEIN PHOSPHORYLATION|VACUOLAR PROTEIN PROCESSING 0.0177013450671085 0.305362270037863 1132 Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) vacuolar protein processing protein serine/threonine kinase activity fungal-type vacuole membrane YKL033W-A_p YKL033W-A hom 0.0176962964619197 0.305428130359293 1133 Putative protein of unknown function; similar to uncharacterized proteins from other fungi biological_process molecular_function cellular_component YER090W TRP2 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0176819644194049 0.307194197937553 1134 Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p tryptophan biosynthetic process anthranilate synthase activity anthranilate synthase complex|cytoplasm YDL046W NPC2 hom 0.0176638589199884 0.306676509385491 1135 Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes intracellular sterol transport molecular_function fungal-type vacuole lumen|fungal-type vacuole YHR205W SCH9 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|PROTEIN PHOSPHORYLATION|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.0176340479449485 0.307132809132487 1136 AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan protein phosphorylation|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|regulation of protein localization|positive regulation of transcription from RNA polymerase III promoter|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase I promoter|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|regulation of cell size|regulation of response to osmotic stress protein serine/threonine kinase activity chromatin|fungal-type vacuole membrane|nucleus|cytoplasm YKL083W_d YKL083W het 0.0176255250085994 0.307366699391944 1137 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 Unknown Unknown Unknown YDL033C SLM3 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0175077184998454 0.311044908367702 1138 tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) mitochondrial tRNA thio-modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity mitochondrion YBR144C_d YBR144C hom 0.0174940005547477 0.310990994497624 1139 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Unknown Unknown Unknown YDR422C SIP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION 0.0174529248225016 0.312128631334632 1140 Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions signal transduction|protein phosphorylation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|fungal-type vacuole YDR191W HST4 hom FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0174433590551014 0.312393958649375 1141 Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|chromatin silencing at telomere|histone deacetylation histone deacetylase activity|DNA binding nucleus|cytoplasm YPL081W RPS9A hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS|RIBOSOME 0.017434637045451 0.312636011930906 1142 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome|rRNA binding small-subunit processome|cytosolic small ribosomal subunit|90S preribosome|cytoplasm YFL047W RGD2 hom FT SIGNALING|SITE OF POLARIZED GROWTH 0.0174099427277484 0.313321998381907 1143 GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress small GTPase mediated signal transduction Rho GTPase activator activity cellular bud neck|cytoplasm YDR394W RPT3 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.0173607416761614 0.314691710417912 1144 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YHR181W SVP26 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0173342710169405 0.315430254579189 1145 Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment protein retention in Golgi apparatus|fungal-type cell wall organization|protein glycosylation|ER to Golgi vesicle-mediated transport COPII adaptor activity integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|integral to Golgi membrane|ER to Golgi transport vesicle YNL020C ARK1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0173178046267689 0.315962413357802 1146 Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|cytokinesis|actin cortical patch assembly protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YLR380W CSR1 hom FT KETONE METABOLISM|LIPID METABOLISM|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0173107897649776 0.316086344599484 1147 Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress phosphatidylinositol metabolic process|Golgi to plasma membrane protein transport|Golgi to plasma membrane transport|Unknown|negative regulation of fatty acid biosynthetic process|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cytosol|endosome|mitochondrion|lipid particle YDR309C GIC2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0173052754002762 0.316673865746561 1148 Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region regulation of exit from mitosis|establishment of cell polarity|axial cellular bud site selection|Rho protein signal transduction Rho GTPase binding|Rho GTPase activator activity|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|actin cap|plasma membrane|mating projection tip|cellular bud tip YNL230C ELA1 hom FT PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0172960850869971 0.316497663867615 1149 Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YGL235W_p YGL235W hom 0.017283074436643 0.316861890396645 1150 Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 biological_process molecular_function cellular_component YOL046C_d YOL046C hom 0.0172447363973396 0.317936741701777 1151 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Unknown Unknown Unknown YCL038C ATG22 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT 0.0172368219469758 0.318158929519035 1152 Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation amino acid export from vacuole molecular_function integral to fungal-type vacuolar membrane|integral to membrane YEL019C MMS21 het FT RESPONSE TO DNA DAMAGE|ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME 0.0172347974429144 0.318215781143673 1153 SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination DNA repair SUMO ligase activity nuclear envelope|Smc5-Smc6 complex|nucleus|cytoplasm YMR316C-B_d YMR316C-B hom 0.0172280385662808 0.318405630425348 1154 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCL051W LRE1 hom FT CELL WALL ORG/BIOGENESIS 0.0172097627733809 0.318919348255622 1155 Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication fungal-type cell wall organization molecular_function cellular_component YOR051C ETT1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEUS 0.0171935505366158 0.319375514881185 1156 Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough translational termination molecular_function nucleus YIL137C TMA108 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|PROTEOLYSIS 0.0171825679033163 0.320045810139125 1157 Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease ribosome biogenesis molecular_function ribosome|cytoplasm YCR028C-A RIM1 hom FT MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0171724693573631 0.319969317755908 1158 Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication mitochondrial DNA replication|mitochondrial genome maintenance single-stranded DNA binding mitochondrial nucleoid|mitochondrion YGL111W NSA1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0171685841113215 0.32007883390355 1159 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YIL018W RPL2B hom FT TRANSLATION|RIBOSOME 0.0171622486382128 0.320257468857106 1160 Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YBR235W VHC1 hom 0.017150930095716 0.32057676853354 1161 Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family transmembrane transport ion transmembrane transporter activity integral to membrane YKL092C BUD2 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0171316911322308 0.321119982820897 1162 GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint bipolar cellular bud site selection|small GTPase mediated signal transduction|filamentous growth|cellular bud site selection|axial cellular bud site selection GTPase activator activity|Ras GTPase activator activity intracellular YJR140C HIR3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS 0.0171177378048736 0.321514332372091 1163 Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding|transcription corepressor activity HIR complex|nucleus YJR069C HAM1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0171008290195788 0.321992632617007 1164 Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress purine deoxyribonucleoside triphosphate catabolic process|pyrimidine deoxyribonucleoside triphosphate catabolic process dITP diphosphatase activity|ITP diphosphatase activity|XTP diphosphatase activity|dCTP diphosphatase activity|UTP diphosphatase activity|dTTP diphosphatase activity|dATP pyrophosphohydrolase activity|dGTP diphosphatase activity|dUTP diphosphatase activity|GTP diphosphatase activity nucleus|cytoplasm YKL096W CWP1 hom FT MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS 0.0170874190147952 0.322372292442379 1165 Cell wall mannoprotein that localizes specifically to birth scars of daughter cells, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance fungal-type cell wall organization|ascospore-type prospore membrane assembly structural constituent of cell wall fungal-type cell wall|cellular birth scar|prospore membrane YPL018W CTF19 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0170857815198082 0.322418672675923 1166 Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 attachment of spindle microtubules to kinetochore|chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function COMA complex|condensed nuclear chromosome kinetochore|nucleus YDL085C-A_p YDL085C-A hom FT NUCLEUS 0.0170834299744159 0.322485285207873 1167 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YOL026C MIM1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0170760150461381 0.322695387600789 1168 Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane molecular_function mitochondrial outer membrane|integral to mitochondrial outer membrane YGR137W_d YGR137W hom 0.0170700680073703 0.322936117623978 1169 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR089W YDR089W hom FT MEMBRANE ORGANIZATION 0.0170502775523092 0.323641878566569 1170 Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress cellular membrane organization molecular_function integral to membrane|membrane YBR256C RIB5 het FT OXIDATION-REDUCTION PROCESS 0.0170381019572848 0.32377105130952 1171 Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway riboflavin biosynthetic process riboflavin synthase activity cellular_component YJR010W MET3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0170368218937599 0.323807409757387 1172 ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process sulfate adenylyltransferase (ATP) activity mitochondrion|cytoplasm YKR018C YKR018C hom FT NUCLEUS|MITOCHONDRION 0.0170336882634695 0.323896427446593 1173 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YDL079C MRK1 hom FT PROTEIN PHOSPHORYLATION 0.0169996865471938 0.324863345662422 1174 Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation regulation of protein catabolic process|protein phosphorylation|response to stress protein serine/threonine kinase activity cellular_component YPR006C ICL2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.016973412644854 0.325828286459969 1175 2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol threonine catabolic process|propionate metabolic process methylisocitrate lyase activity mitochondrion|mitochondrial matrix YLR131C ACE2 hom FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|NUCLEUS 0.0169443477849113 0.32709106326667 1176 Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YNL170W_d YNL170W hom 0.0168612903056215 0.32881832021084 1177 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR060W CBF1 hom FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME|KINETOCHORE 0.0168612336459914 0.328819945746029 1178 Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress regulation of transcription from RNA polymerase II promoter in response to methionine|negative regulation of transcription from RNA polymerase II promoter|methionine biosynthetic process|chromosome segregation|Unknown|chromatin remodeling|negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity|RNA polymerase II repressing transcription factor binding|centromeric DNA binding chromosome, centromeric region|mitochondrion|nucleus|kinetochore YNL277W-A_p YNL277W-A hom 0.016851462527294 0.329100351913518 1179 Putative protein of unknown function biological_process molecular_function cellular_component YLR334C_d YLR334C hom 0.0168405854303388 0.329412678969088 1180 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Unknown Unknown Unknown YGR035W-A_p YGR035W-A hom 0.0168322141364849 0.329653184581055 1181 Putative protein of unknown function biological_process molecular_function cellular_component YDR236C FMN1 het FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0168290501146063 0.330249384997415 1182 Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane FMN biosynthetic process riboflavin kinase activity|FMN adenylyltransferase activity intracellular membrane-bounded organelle|mitochondrial inner membrane YPR072W NOT5 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.0167885415817165 0.330909731461463 1183 Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|cytoplasm YOL008W COQ10 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0167826581225353 0.331079246774237 1184 Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes cellular respiration ubiquinone binding mitochondrion|mitochondrial inner membrane YCL041C_d YCL041C het 0.0167824578456762 0.331085018175989 1185 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Unknown Unknown Unknown YER019C-A SBH2 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0167726193490917 0.331729351429826 1186 Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p SRP-dependent cotranslational protein targeting to membrane ARF guanyl-nucleotide exchange factor activity Ssh1 translocon complex YMR244C-A COA6 hom FT NUCLEUS|MITOCHONDRION 0.0167606000603373 0.333524135248761 1187 Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YHR150W PEX28 hom FT PEROXISOME ORGANIZATION 0.016745240383187 0.332230745748118 1188 Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p peroxisome organization molecular_function integral to membrane|peroxisomal membrane YJR122W IBA57 hom FF|FT COFACTOR METABOLISM|MITOCHONDRION 0.0167218429565739 0.333484335549164 1189 Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system iron-sulfur cluster assembly molecular_function mitochondrion|mitochondrial matrix YHR034C PIH1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0167149929076763 0.333177066087192 1190 Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|rRNA processing|regulation of cell size|box C/D snoRNP assembly molecular_function R2TP complex|nucleus|small nucleolar ribonucleoprotein complex|cytoplasm YEL007W MIT1 hom FT NUCLEUS 0.016679269126226 0.334067268473937 1191 Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 biological_process molecular_function nucleus|cytoplasm YGL014W PUF4 hom FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|TRANSLATION|GENE SILENCING 0.0166781807195427 0.334098816440489 1192 Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|negative regulation of translation|protein localization|negative regulation of chromatin silencing involved in replicative cell aging mRNA binding cytoplasm YNL027W CRZ1 hom FT TRANSCRIPTION FROM RNA POL II|SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|NUCLEUS 0.0166593774381562 0.334644141301315 1193 Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress regulation of potassium ion concentration by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to calcium ion|positive regulation of sodium ion transport by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|cell wall chitin biosynthetic process|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter by pheromones|ion homeostasis|Unknown|positive regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YGL153W PEX14 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0166546563137501 0.334781151373198 1194 Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p protein import into peroxisome matrix, docking protein binding, bridging peroxisomal membrane YKL161C KDX1 hom FT PROTEIN PHOSPHORYLATION 0.0166529931944748 0.33511784015159 1195 Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p biological_process protein kinase activity cellular_component YCR101C_p YCR101C hom 0.016651588191685 0.33494228190831 1196 Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene biological_process molecular_function integral to membrane|membrane YDR404C RPB7 het FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|TRANSLATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0166484599692165 0.335105191519339 1197 RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation transcription initiation from RNA polymerase II promoter|positive regulation of translational initiation|positive regulation of nuclear-transcribed mRNA poly(A) tail shortening|nuclear-transcribed mRNA catabolic process, exonucleolytic|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|translation initiation factor binding|single-stranded RNA binding|single-stranded DNA binding nucleus|DNA-directed RNA polymerase II, core complex|cytoplasmic mRNA processing body|cytoplasm YOL051W GAL11 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0166295830157937 0.3358699346952 1198 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|TFIIE-class binding transcription factor activity|RNA polymerase II activating transcription factor binding|TFIIE-class transcription factor binding|TFIIH-class transcription factor binding|RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex YNL270C ALP1 hom FT PLASMA MEMBRANE 0.0166216261560331 0.335812781253918 1199 Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication basic amino acid transport basic amino acid transmembrane transporter activity integral to membrane|plasma membrane YDR213W UPC2 hom FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|NUCLEUS 0.0165947682644595 0.336522276555707 1200 Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication cellular response to hypoxia|positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|membrane|cytoplasm|perinuclear region of cytoplasm YNL189W SRP1 het FT PROTEIN LOCALIZATION|NUCLEUS 0.0165804566564771 0.337155428531788 1201 Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation protein targeting to membrane|protein import into nucleus protein transporter activity integral to membrane|nuclear exosome (RNase complex)|nucleus|cytoplasm YDL011C_d YDL011C hom 0.0165393027164845 0.338789067589589 1202 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Unknown Unknown Unknown YOR196C LIP5 hom FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0165200410436802 0.338702970638609 1203 Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase protein lipoylation lipoate synthase activity mitochondrion YAL054C ACS1 hom FT KETONE METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.016505599520573 0.339197469613665 1204 Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions acetate fermentation|histone acetylation|acetyl-CoA biosynthetic process acetate-CoA ligase activity|acid-ammonia (or amide) ligase activity cytosol|mitochondrion|integral to membrane YDR412W RRP17 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0164900100881182 0.339581883778192 1205 Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA rRNA processing molecular_function cytoplasm YLR336C SGD1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.016480949419186 0.339847348340777 1206 Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia ribosomal small subunit biogenesis|hyperosmotic response molecular_function nucleolus|nucleus|FAl1-SGD1 complex YMR039C SUB1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0164644338491095 0.340331572801875 1207 Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress termination of RNA polymerase II transcription|hyperosmotic response|RNA polymerase III transcriptional preinitiation complex assembly|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase III promoter transcription coactivator activity nucleus YDR220C_d YDR220C hom 0.0164596856955775 0.340470866943477 1208 Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YOR250C CLP1 het FT RNA PROCESSING|NUCLEUS 0.0164363339502495 0.341156456338687 1209 Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation mRNA 3'-end processing|mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage factor complex YGR255C COQ6 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0164261835879102 0.341454738505774 1210 Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) ubiquinone biosynthetic process N,N-dimethylaniline monooxygenase activity mitochondrion|mitochondrial inner membrane YCR019W MAK32 hom 0.0163991477538568 0.342250036936543 1211 Protein necessary for structural stability of L-A double-stranded RNA-containing particles interspecies interaction between organisms molecular_function cellular_component YLR415C_p YLR415C hom FT MITOCHONDRION 0.0163976314757733 0.342294675504553 1212 Putative protein of unknown function; YLR415C is not an essential gene biological_process molecular_function cellular_component YJR031C GEA1 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.016397398554936 0.342301532922546 1213 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|Golgi-associated vesicle|extrinsic to membrane YOR299W BUD7 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|GOLGI APPARATUS 0.0163920839617587 0.342746052287088 1214 Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process|cellular bud site selection molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YDR331W GPI8 het FT LIPID METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.016371055209652 0.343077673437833 1215 ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog attachment of GPI anchor to protein GPI-anchor transamidase activity endoplasmic reticulum membrane|GPI-anchor transamidase complex YHR007C ERG11 het FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.0163378092107007 0.34405878637837 1216 Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p ergosterol biosynthetic process sterol 14-demethylase activity endoplasmic reticulum YPL032C SVL3 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|SITE OF POLARIZED GROWTH 0.0163065380463804 0.345703542457202 1217 Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress endocytosis molecular_function cellular bud|cellular bud neck|cytoplasm YIL021W RPB3 het FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0163049094285121 0.345319311574053 1218 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity nucleoplasm|DNA-directed RNA polymerase II, core complex YKL070W_p YKL070W hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0162881170236324 0.345816408325841 1219 Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDL065C PEX19 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0162814005160776 0.345943374948199 1220 Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning protein stabilization|protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization|protein exit from endoplasmic reticulum peroxisome membrane targeting sequence binding|protein binding cytosol|peroxisomal membrane|endoplasmic reticulum YNL285W_d YNL285W hom 0.0162713877168111 0.346024287004626 1221 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR424W SPP382 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0162706389438786 0.346046484872578 1222 Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation spliceosomal complex disassembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step RNA binding|ATP-dependent RNA helicase activity mitochondrion|U2-type post-mRNA release spliceosomal complex|nucleus|spliceosomal complex|cytoplasm YDL104C QRI7 hom FT RNA PROCESSING|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0162690962183869 0.346092222847062 1223 Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification mitochondrial tRNA threonylcarbamoyladenosine modification molecular_function mitochondrion YMR147W_p YMR147W hom 0.0162356776753763 0.347083944260437 1224 Putative protein of unknown function biological_process molecular_function cellular_component YDL112W TRM3 hom FT RNA PROCESSING 0.0162194855339681 0.347565108188582 1225 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs tRNA methylation tRNA (guanine) methyltransferase activity cytoplasm YML094C-A_d YML094C-A hom FF 0.0162125222291779 0.347772159344681 1226 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Unknown Unknown Unknown YBL029C-A YBL029C-A hom FT PLASMA MEMBRANE 0.0162017098259139 0.348093817300957 1227 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica biological_process molecular_function plasma membrane YMR164C MSS11 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0161972876948153 0.34822542563788 1228 Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YNL239W LAP3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS|MITOCHONDRION 0.0161933480687387 0.348342700626582 1229 Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH response to antibiotic|negative regulation of transcription from RNA polymerase II promoter|homocysteine catabolic process double-stranded DNA binding|cysteine-type peptidase activity|mRNA binding|single-stranded DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding mitochondrion|cytoplasm YJR010C-A SPC1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0161877962535273 0.348508009931114 1230 Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress signal peptide processing|protein targeting to ER molecular_function signal peptidase complex YIL129C TAO3 het FT CELL DIVISION|MITOCHONDRION|SITE OF POLARIZED GROWTH 0.0161870953820835 0.348528882425869 1231 Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p cell morphogenesis|cell budding|budding cell apical bud growth molecular_function incipient cellular bud site|mitochondrion|cellular bud|mating projection tip YMR185W RTP1 het 0.016179027080679 0.348769219873075 1232 Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene biological_process molecular_function cellular_component YMR012W CLU1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION 0.0161735218725457 0.348933268639098 1233 eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant translational initiation|mitochondrion organization molecular_function eukaryotic translation initiation factor 3 complex|cytoplasm YBR296C-A_p YBR296C-A hom 0.0161711184502215 0.349004903174422 1234 Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YGR198W YPP1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|PLASMA MEMBRANE|CYTOSKELETON 0.0161552824942779 0.350052569280779 1235 Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene receptor-mediated endocytosis|response to pheromone|protein targeting to vacuole molecular_function endosome|cytosol|ribosome|plasma membrane|actin cortical patch|cytoplasm YJL036W SNX4 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT 0.0161429182397786 0.349846114040467 1236 Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p piecemeal microautophagy of nucleus|mitochondrion degradation|early endosome to Golgi transport|CVT pathway|protein targeting to vacuole phosphatidylinositol-3-phosphate binding cytosol|pre-autophagosomal structure|extrinsic to membrane|early endosome YMR175W-A_p YMR175W-A hom 0.0161385605115224 0.349976219485988 1237 Putative protein of unknown function biological_process molecular_function cellular_component YIR023W DAL81 hom FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0161220203177627 0.350470326960601 1238 Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism nitrogen utilization|nitrogen catabolite activation of transcription from RNA polymerase II promoter|gamma-aminobutyric acid catabolic process|urea catabolic process RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor recruiting transcription factor activity nucleus YIL067C_p YIL067C hom 0.0161138875655454 0.350713439869529 1239 Uncharacterized protein of unknown function biological_process molecular_function integral to membrane|fungal-type vacuole YKL001C MET14 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.016088332446806 0.351478056071384 1240 Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process adenylylsulfate kinase activity cytoplasm YJL198W PHO90 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0160590917811876 0.352354238727507 1241 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication phosphate ion transport phosphate ion transmembrane transporter activity integral to membrane|membrane YKL185W ASH1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.016056477990461 0.35243262694995 1242 Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|core promoter proximal region sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|cellular bud|Rpd3L complex YNL301C RPL18B hom FT TRANSLATION|RIBOSOME 0.01601963025355 0.353538873368629 1243 Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YIL020C HIS6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0160193475912152 0.353547367956678 1244 Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts histidine biosynthetic process 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity intracellular YLR059C REX2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0160019638653138 0.35407003273374 1245 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease U5 snRNA 3'-end processing|U4 snRNA 3'-end processing|mitochondrial genome maintenance|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 3'-5'-exoribonuclease activity|3'-5' exonuclease activity mitochondrion YMR279C YMR279C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0159979306940393 0.354191365082374 1246 Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication borate transmembrane transport borate efflux transmembrane transporter activity integral to membrane YKL218C SRY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0159948508205811 0.354714917452428 1247 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity cellular modified amino acid catabolic process serine racemase activity|threo-3-hydroxyaspartate ammonia-lyase activity cellular_component YHR058C MED6 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0159798787401364 0.35502189052508 1248 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly core mediator complex YNL179C_d YNL179C hom 0.0159741285600247 0.354907953760597 1249 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YDR519W FPR2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0159707758614056 0.355008963786663 1250 Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress biological_process FK506 binding|peptidyl-prolyl cis-trans isomerase activity endoplasmic reticulum membrane YOR063W RPL3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.0159481585071849 0.355690852724024 1251 Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YLR046C_p YLR046C hom 0.0159468326076742 0.355730852776771 1252 Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function integral to membrane YDL060W TSR1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0159457843452163 0.355762479015939 1253 Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress rRNA processing|ribosome biogenesis ribonucleoprotein complex binding nucleolus|cytoplasm|90S preribosome YIL043C CBR1 hom FT OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0159307933548108 0.35621495341317 1254 Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia biological_process cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion YOR040W GLO4 hom FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.015911558592299 0.356796048837656 1255 Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate cellular carbohydrate metabolic process|methylglyoxal catabolic process to D-lactate hydroxyacylglutathione hydrolase activity mitochondrion|mitochondrial matrix YGR002C SWC4 het FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0158965359483692 0.3572503081999 1256 Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex DNA repair|histone acetylation|chromosome organization|chromatin modification|chromatin remodeling|histone exchange|establishment of mitotic sister chromatid cohesion DNA binding Swr1 complex|histone acetyltransferase complex|nucleus|NuA4 histone acetyltransferase complex YAL033W POP5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0158926040055138 0.357369263589778 1257 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YHR060W VMA22 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0158835707896886 0.357642645213424 1258 Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) vacuolar proton-transporting V-type ATPase complex assembly|vacuolar acidification unfolded protein binding extrinsic to endoplasmic reticulum membrane YER053C-A YER053C-A hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.015873547864176 0.357946133406752 1259 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress biological_process molecular_function endoplasmic reticulum YOR033C EXO1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0158701789918134 0.358048177210627 1260 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress mismatch repair|telomeric 3' overhang formation|DNA double-strand break processing|meiotic DNA double-strand break processing|telomere maintenance|gene conversion at mating-type locus, DNA double-strand break processing 5'-3' exonuclease activity|5'-flap endonuclease activity nucleus YOR013W_d IRC11 hom 0.015860217468904 0.358565099556102 1261 Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YER020W GPA2 hom FT SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.0158299335946158 0.359268632660914 1262 Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery adenylate cyclase-activating G-protein coupled receptor signaling pathway|ascospore formation|replicative cell aging|signal transduction|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|regulation of cell size GTPase activity mitochondrion|plasma membrane YER048W-A ISD11 het FT COFACTOR METABOLISM|MITOCHONDRION 0.0158028098537062 0.360092641572615 1263 Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis iron-sulfur cluster assembly enzyme activator activity extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial matrix YPR177C_d YPR177C het 0.0158025598363953 0.36017187411924 1264 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Unknown Unknown Unknown YNL214W PEX17 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION 0.0157724258013352 0.361017104777883 1265 Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis protein import into peroxisome matrix, docking protein binding peroxisomal membrane YDR354W TRP4 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0157703668839124 0.361940389020974 1266 Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis tryptophan biosynthetic process anthranilate phosphoribosyltransferase activity nucleus|cytoplasm YPL150W_p YPL150W hom FT PROTEIN PHOSPHORYLATION 0.0157499381044224 0.36170227129784 1267 Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study protein phosphorylation protein kinase activity cellular_component YML085C TUB1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0157076919773015 0.363206385385672 1268 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|cytoplasmic microtubule|kinetochore microtubule|nuclear microtubule|spindle pole body|polar microtubule YDL090C RAM1 hom FF|FT PROTEIN PRENYLATION 0.0157072373993278 0.364512384188931 1269 Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit protein farnesylation protein farnesyltransferase activity protein farnesyltransferase complex YDL238C GUD1 hom 0.0156953987867284 0.363367384016164 1270 Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures guanine metabolic process guanine deaminase activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides cytoplasm YOL029C_p YOL029C hom 0.015686516510061 0.363639017634793 1271 Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component YDR062W LCB2 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0156691871594465 0.364169341074694 1272 Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine sphingolipid biosynthetic process serine C-palmitoyltransferase activity SPOTS complex|serine C-palmitoyltransferase complex YBR079C RPG1 het FT TRANSLATION 0.0156435991466992 0.3649532843297 1273 eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation translation reinitiation|translational initiation translation initiation factor activity incipient cellular bud site|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YJR098C_p YJR098C hom FT LIPID METABOLISM|MITOCHONDRION 0.0156434395322121 0.364958177763898 1274 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YFL040W_p YFL040W hom 0.0156265273809759 0.366694937723801 1275 Putative transporter, member of the sugar porter family; YFL040W is not an essential gene transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YNL099C OCA1 hom FF|FT RESPONSE TO OXIDATIVE STRESS 0.015615916976833 0.365802572144569 1276 Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA cellular response to oxidative stress protein tyrosine phosphatase activity cytoplasm YKL028W TFA1 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION|RNA POL II, HOLOENZYME 0.0156120356696862 0.365921748959327 1277 TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening transcriptional open complex formation at RNA polymerase II promoter|transcription from RNA polymerase II promoter single-stranded DNA binding|TFIIH-class transcription factor binding|RNA polymerase II core binding mitochondrion|transcription factor TFIIE complex YJL013C MAD3 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS 0.0155844007436312 0.366770988390532 1278 Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover distributive segregation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex YGL016W KAP122 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0155479607121698 0.367892690382174 1279 Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance protein import into nucleus|regulation of cell size protein transporter activity integral to membrane|nuclear pore|cytoplasm YBL015W ACH1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0155455723318614 0.36796628431521 1280 Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth acetate metabolic process acetate CoA-transferase activity|acetyl-CoA hydrolase activity cytosol|mitochondrion YDR034C LYS14 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0155442570130354 0.368078241399774 1281 Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YJR049C UTR1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|COFACTOR METABOLISM|NUCLEUS 0.0155350168075621 0.368291644810123 1282 ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication cellular iron ion homeostasis|NADP biosynthetic process NAD+ kinase activity|NADH kinase activity nucleus|cytoplasm YGR239C PEX21 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0155119503677101 0.369003260922989 1283 Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress protein import into peroxisome matrix|positive regulation of protein binding protein binding cytosol|peroxisome YAR008W SEN34 het FT RNA PROCESSING|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0154991587028623 0.369398261119185 1284 Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YLR178C TFS1 hom FT SIGNALING|PROTEOLYSIS 0.0154814167605296 0.369946558183554 1285 Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress regulation of Ras protein signal transduction|regulation of proteolysis lipid binding|peptidase inhibitor activity|phospholipid binding fungal-type vacuole lumen|fungal-type vacuole membrane|cytoplasm YDR175C RSM24 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0154479014651758 0.371340571925915 1286 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YOR114W_p YOR114W hom 0.0154447324026862 0.371081853724782 1287 Putative protein of unknown function; null mutant is viable biological_process molecular_function cellular_component YER056C FCY2 hom FT PLASMA MEMBRANE 0.0154361137086315 0.371348895456119 1288 Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress cytosine transport|cytidine transport|purine nucleobase transport|transmembrane transport nucleobase transmembrane transporter activity|cytidine transmembrane transporter activity integral to membrane|plasma membrane YNL317W PFS2 het FT RNA PROCESSING|NUCLEUS 0.0154332695719982 0.371793854911223 1289 Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YPR047W MSF1 hom FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0153975553909906 0.372545045501325 1290 Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase phenylalanyl-tRNA aminoacylation|mitochondrial phenylalanyl-tRNA aminoacylation phenylalanine-tRNA ligase activity mitochondrion YDL152W_d YDL152W het FF 0.0153887316595098 0.37281910906688 1291 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Unknown Unknown Unknown YAL055W PEX22 hom FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION 0.0153876969801589 0.372851254202945 1292 Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation protein import into peroxisome matrix, receptor recycling|protein targeting to peroxisome protein anchor integral to peroxisomal membrane YML032C RAD52 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0153749154144357 0.373248489924739 1293 Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis postreplication repair|telomere maintenance via recombination|meiotic joint molecule formation|meiotic DNA recombinase assembly|DNA recombinase assembly|DNA strand renaturation|double-strand break repair via synthesis-dependent strand annealing|double-strand break repair via single-strand annealing|double-strand break repair via break-induced replication recombinase activity nuclear chromosome|nucleus YBR198C TAF5 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE 0.0153616407611363 0.374231907601573 1294 Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter ubiquitin binding|chromatin binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YKR064W OAF3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION 0.0153411033763156 0.375228140914126 1295 Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|cellular response to oleic acid RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity mitochondrion|nucleus|cytoplasm YNR075W COS10 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0153368653278733 0.374503822298748 1296 Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins endocytosis molecular_function nuclear envelope|integral to membrane|endoplasmic reticulum|cytoplasm YLR111W_d YLR111W hom FF 0.0153260759583708 0.374768772756754 1297 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YAL032C PRP45 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0152878087161229 0.37596263128218 1298 Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene generation of catalytic spliceosome for second transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity nucleus|post-mRNA release spliceosomal complex|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YGR159C NSR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0152776417108797 0.376280214904575 1299 Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis ribosomal small subunit assembly|rRNA processing single-stranded DNA binding|RNA binding mitochondrion|nucleolus|nucleus YBL007C SLA1 hom FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.0152752533053287 0.377352952968943 1300 Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains fungal-type cell wall organization|endocytosis|actin cortical patch assembly ubiquitin binding|protein binding, bridging cell cortex|nucleus|mating projection tip|actin cortical patch YMR252C_p YMR252C hom FT MITOCHONDRION 0.015218142211035 0.378213210232411 1301 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion YIR027C DAL1 hom 0.0152079092567905 0.378462883395636 1302 Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression allantoin assimilation pathway allantoinase activity cellular_component YER002W NOP16 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0152014685555913 0.378735967607213 1303 Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YOL039W RPP2A hom FT TRANSLATION|RIBOSOME 0.0151503979349734 0.380339912900964 1304 Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL175C AIR2 hom FT RNA PROCESSING|NUCLEUS 0.0151379505314561 0.380660446615201 1305 RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication nuclear polyadenylation-dependent CUT catabolic process|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|tRNA modification polynucleotide adenylyltransferase activity|RNA binding|protein binding, bridging nucleolus|TRAMP complex YPR003C_p YPR003C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0151318825701805 0.380851423647332 1306 Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YMR085W_p YMR085W hom FT CARBOHYDRATE METABOLISM 0.0151274610087598 0.38120372062066 1307 Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component YMR137C PSO2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0151170962510719 0.381317039891402 1308 Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress DNA repair|double-strand break repair damaged DNA binding|5'-3' exonuclease activity nucleus YAL016C-B_d YAL016C-B hom 0.015114493125426 0.381399047586859 1309 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR336W_p YDR336W hom 0.0151090782791596 0.38199591652069 1310 Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene biological_process molecular_function cellular_component YLR110C CCW12 hom FF|FT CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH 0.0150637669964693 0.383212147506373 1311 Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication conjugation with cellular fusion|agglutination involved in conjugation with cellular fusion|fungal-type cell wall organization molecular_function fungal-type cell wall|mating projection tip|cellular bud tip YDR045C RPC11 het FT NUCLEUS 0.0150625919808311 0.383036375441822 1312 RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS termination of RNA polymerase III transcription|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YDR216W ADR1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|PEROXISOME ORGANIZATION|NUCLEUS 0.0150427826470196 0.383662434681463 1313 Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization negative regulation of chromatin silencing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|chromatin organization|regulation of transcription by chromatin organization|positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by oleic acid|cellular response to oleic acid|peroxisome organization|positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|TFIIB-class transcription factor binding|sequence-specific DNA binding|TFIID-class transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription nucleus YLR407W_p YLR407W hom 0.0150272432729166 0.384153983031017 1314 Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus biological_process molecular_function cellular_component YGL229C SAP4 hom FT CELL CYCLE|MITOCHONDRION 0.0150148399907628 0.384546605424383 1315 Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity mitochondrion|cytoplasm YBR115C LYS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0150106386998851 0.384679651703268 1316 Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p lysine biosynthetic process via aminoadipic acid L-aminoadipate-semialdehyde dehydrogenase activity cytoplasm YPR086W SUA7 het FT TRANSCRIPTION FROM RNA POL II|TRANSLATION|NUCLEUS 0.0149910524554068 0.385654768201904 1317 Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II transcriptional start site selection at RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|Unknown TBP-class protein binding|RNA polymerase II core binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|core RNA polymerase II recruiting transcription factor activity nucleus YPL161C BEM4 hom FF|FT SIGNALING|CYTOSKELETON ORGANIZATION|NUCLEUS 0.0149845649783916 0.385505982101983 1318 Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length establishment of cell polarity|Rho protein signal transduction|actin cytoskeleton organization molecular_function nucleus|cytoplasm YMR282C AEP2 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0149831671727378 0.385550312061127 1319 Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader mitochondrial translational initiation molecular_function mitochondrion YPL125W KAP120 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0149830876237539 0.385552834968411 1320 Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p protein import into nucleus|transcription factor import into nucleus protein transporter activity|structural constituent of nuclear pore integral to membrane|nucleus|nuclear pore|cytoplasm YDR290W_d YDR290W hom 0.0149445574126697 0.386776010870792 1321 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Unknown Unknown Unknown YKL128C PMU1 hom FT NUCLEUS 0.0149286755554763 0.387280882625002 1322 Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant biological_process molecular_function nucleus|cytoplasm YER116C SLX8 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0149095386545478 0.38788976300623 1323 Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress protein sumoylation|telomere maintenance|response to DNA damage stimulus|protein ubiquitination ubiquitin-protein ligase activity ubiquitin ligase complex|nucleus YGR154C GTO1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.0149001176524269 0.388189725887852 1324 Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization glutathione metabolic process glutathione transferase activity peroxisome YDR344C_d YDR344C hom 0.0148933128502846 0.388406477302869 1325 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL182C_d YJL182C hom 0.0148848115794732 0.388677369345264 1326 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Unknown Unknown Unknown YOL028C YAP7 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0148817522185335 0.388774883667341 1327 Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nucleus YIL015W BAR1 hom FT SIGNALING|PROTEOLYSIS 0.0148427626600297 0.390018943550174 1328 Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|peptide catabolic process aspartic-type endopeptidase activity cell wall-bounded periplasmic space YNL067W-B_p YNL067W-B hom 0.0148419717038723 0.390044206005125 1329 Putative protein of unknown function biological_process molecular_function cellular_component YOR242C SSP2 hom FT CELL WALL ORG/BIOGENESIS 0.0148110755299933 0.391314460325638 1330 Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis ascospore wall assembly|ascospore formation molecular_function ascospore wall YOL011W PLB3 hom FT LIPID METABOLISM|PLASMA MEMBRANE 0.0148000737430812 0.391454440113692 1331 Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro phosphatidylinositol metabolic process|phosphatidylserine catabolic process lysophospholipase activity extracellular region|plasma membrane YDL237W AIM6 hom 0.0147977856908717 0.391457047916421 1332 Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene biological_process molecular_function cellular_component YAL027W SAW1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0147909589866281 0.39167560747071 1333 5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus double-strand break repair via single-strand annealing, removal of nonhomologous ends molecular_function nucleus YEL051W VMA8 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0147839592914759 0.391899782234921 1334 Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis vacuolar acidification proton-transporting ATPase activity, rotational mechanism fungal-type vacuole membrane|vacuolar proton-transporting V-type ATPase, V1 domain|integral to membrane YBR067C TIP1 hom FT CELL WALL ORG/BIOGENESIS 0.0147647456858081 0.392515523905676 1335 Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins fungal-type cell wall organization structural constituent of cell wall|lipase activity fungal-type cell wall YJL217W REE1 hom 0.0147627623362597 0.392579118010515 1336 Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle biological_process molecular_function cytoplasm YOL164W-A_p YOL164W-A hom 0.0147564331509341 0.392852671411737 1337 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YOR365C_p YOR365C hom 0.0147358557985069 0.393513011821994 1338 Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication biological_process molecular_function integral to membrane YDL225W SHS1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0147329669868739 0.393888120359482 1339 Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress cytokinesis, actomyosin contractile ring assembly|G1 phase of mitotic cell cycle|barrier septum assembly|exit from mitosis|cell cycle cytokinesis|septin ring assembly|cellular bud neck septin ring organization GTP binding cellular bud neck septin ring|mating projection tip YDR014W RAD61 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0147298172874936 0.393636380784867 1340 Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin mitotic sister chromatid cohesion molecular_function nuclear cohesin complex YOR304W ISW2 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0147294956955376 0.393646709705135 1341 ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth negative regulation of transcription from RNA polymerase II promoter by pheromones|nucleosome positioning|termination of RNA polymerase II transcription|negative regulation of antisense RNA transcription|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere single-stranded DNA binding|DNA translocase activity chromatin accessibility complex|nucleus YOR317W FAA1 hom FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0147264053370547 0.393745974487546 1342 Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication long-chain fatty acid transport|long-chain fatty-acyl-CoA metabolic process long-chain fatty acid-CoA ligase activity mitochondrial outer membrane|mitochondrion|lipid particle|plasma membrane YLR283W_p YLR283W hom FT MITOCHONDRION 0.0147149282273511 0.394467536023807 1343 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene biological_process molecular_function mitochondrion YBR072W HSP26 hom FT NUCLEUS 0.014711009522519 0.39424072602954 1344 Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity protein folding|response to stress unfolded protein binding|mRNA binding nucleus|cytoplasm YJL214W HXT8 hom FT PLASMA MEMBRANE 0.0147060865617497 0.394540039178576 1345 Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose hexose transport mannose transmembrane transporter activity|glucose transmembrane transporter activity|fructose transmembrane transporter activity integral to membrane|plasma membrane YOL083W ATG34 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS 0.0146703209637168 0.39576151338492 1346 Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog protein complex assembly|protein complex localization protein binding, bridging CVT complex YML075C HMG1 hom FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0146496718381356 0.396567910972516 1347 HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress ergosterol biosynthetic process|isopentenyl diphosphate biosynthetic process, mevalonate pathway hydroxymethylglutaryl-CoA reductase (NADPH) activity nuclear envelope|integral to membrane|endoplasmic reticulum membrane YMR291W TDA1 hom FT PROTEIN PHOSPHORYLATION|NUCLEUS 0.0146442395129238 0.396390758858276 1348 Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress protein phosphorylation protein kinase activity nucleus|cytoplasm YFR011C AIM13 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0146427877929785 0.396437583528645 1349 Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial crista junction|cytoplasm|mitochondrial intermembrane space YOR017W PET127 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0146066443508065 0.39760445203133 1350 Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane mitochondrial RNA 5'-end processing molecular_function mitochondrial envelope|mitochondrion YDL132W CDC53 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0146048003652232 0.39773441023629 1351 Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|G2/M transition of mitotic cell cycle|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity|DNA replication origin binding|protein binding, bridging SCF ubiquitin ligase complex YML069W POB3 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0145979125772967 0.397886661981356 1352 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress DNA replication-independent nucleosome organization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|regulation of transcription by chromatin organization|DNA-dependent DNA replication chromatin binding|nucleosome binding|histone binding nuclear chromatin|FACT complex|replication fork protection complex|alpha DNA polymerase:primase complex YBR195C MSI1 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.0145833870836653 0.398356391557088 1353 Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase DNA replication-dependent nucleosome assembly histone binding nucleus|CAF-1 complex|cytoplasm YGL079W_p KXD1 hom 0.0145774273802126 0.398549214712586 1354 Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome biological_process molecular_function endosome YJL126W NIT2 hom 0.0145719064581342 0.398727891442678 1355 Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member biological_process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component YPL222W_p FMP40 hom FT MITOCHONDRION 0.0145623260288178 0.399389802622307 1356 Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YPR099C_d YPR099C hom 0.0145600788570459 0.399462561247433 1357 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Unknown Unknown Unknown YCR022C_d YCR022C hom 0.0145527739378335 0.399347460953739 1358 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Unknown Unknown Unknown YDL118W_d YDL118W hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0145405685817935 0.399743010027862 1359 Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component YDL027C_p YDL027C hom FT MITOCHONDRION 0.0145131206437645 0.400633398016597 1360 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene biological_process molecular_function mitochondrion YHR197W RIX1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0144938513981895 0.401259187081434 1361 Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene ribosomal large subunit assembly|ribosomal subunit export from nucleus|rRNA processing molecular_function nucleoplasm|nucleus YEL031W SPF1 hom FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0144922380321485 0.401662852914672 1362 P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 cellular calcium ion homeostasis|transmembrane transport ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|endoplasmic reticulum membrane|cis-Golgi network YNR043W MVD1 het FT LIPID METABOLISM 0.0144647894557313 0.402204113064494 1363 Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer sterol biosynthetic process diphosphomevalonate decarboxylase activity cytoplasm YDL135C RDI1 hom FT MEMBRANE ORGANIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.014455437250907 0.402789222418069 1364 Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|actin filament organization|small GTPase mediated signal transduction Rho GDP-dissociation inhibitor activity cytosol|plasma membrane|cellular bud neck|cellular bud tip YBR026C ETR1 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.014450888167631 0.402656573661429 1365 2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis fatty acid biosynthetic process|aerobic respiration enoyl-[acyl-carrier-protein] reductase activity mitochondrion YNL118C DCP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0144450206065052 0.402847643156573 1366 Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|deadenylation-independent decapping of nuclear-transcribed mRNA|stress granule assembly hydrolase activity|mRNA binding|m7G(5')pppN diphosphatase activity nucleus|cytoplasmic mRNA processing body|cytoplasm YGR106C VOA1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0144335681981204 0.403571550044552 1367 Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval protein complex assembly molecular_function fungal-type vacuole membrane|endoplasmic reticulum membrane YDR194W-A_p YDR194W-A hom 0.0144107019258379 0.404316922986726 1368 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHR198C AIM18 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0143977843469531 0.404387808172612 1369 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YHR151C MTC6 hom 0.0143898375386883 0.404647263953932 1370 Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YNL009W IDP3 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0143830974705991 0.405077612024039 1371 Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication NADPH regeneration|fatty acid beta-oxidation|isocitrate metabolic process isocitrate dehydrogenase (NADP+) activity mitochondrion|peroxisome|cytoplasm YER107C GLE2 hom FF|FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0143743129228704 0.405154415010978 1372 RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 poly(A)+ mRNA export from nucleus|Unknown|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|nuclear pore distribution|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery molecular_function integral to membrane|nuclear pore|cytoplasm YGR153W_p YGR153W hom 0.0143660672206227 0.405493964091348 1373 Putative protein of unknown function biological_process molecular_function cellular_component YBL067C UBP13 hom FT PROTEOLYSIS 0.0143651296143245 0.405454589822791 1374 Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication biological_process ubiquitin-specific protease activity cellular_component YNL055C POR1 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0143607737039515 0.405597017901247 1375 Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication DNA transport|mitochondrion organization|apoptotic process|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YBR078W ECM33 hom FF|FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION|PLASMA MEMBRANE 0.0143596653680568 0.405633262659804 1376 GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p fungal-type cell wall organization molecular_function mitochondrion|fungal-type cell wall|plasma membrane YGL224C SDT1 hom 0.0143534573438654 0.405836312986466 1377 Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives pyrimidine nucleobase metabolic process nucleotidase activity cellular_component YHR062C RPP1 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0143530369983914 0.408169700754949 1378 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YGR037C ACB1 hom FF|FT KETONE METABOLISM|LIPID METABOLISM|NUCLEUS 0.0143123738468151 0.407181589459515 1379 Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress long-chain fatty acid transport|chronological cell aging|fatty acid metabolic process long-chain fatty acid transporter activity|fatty-acyl-CoA binding extracellular region|nucleus|cytoplasm YER046W-A_d YER046W-A hom 0.0143016130974385 0.407534388326618 1380 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR099W BMH2 hom FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.014294570781348 0.40776537427307 1381 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|ascospore formation|Ras protein signal transduction|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|DNA damage checkpoint|DNA replication initiation|signal transduction involved in filamentous growth|pseudohyphal growth|fungal-type cell wall chitin biosynthetic process|pre-replicative complex assembly DNA replication origin binding|phosphoserine binding nucleus|plasma membrane YOL060C MAM3 hom FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS 0.0142908445871543 0.407887623916542 1382 Protein required for normal mitochondrial morphology, has similarity to hemolysins cellular manganese ion homeostasis|mitochondrion organization molecular_function fungal-type vacuole membrane|integral to membrane YMR280C CAT8 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0142867575779438 0.408021736343497 1383 Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|positive regulation of glyoxylate cycle by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YJR007W SUI2 het FF|FT TRANSLATION|RIBOSOME 0.0142839411840462 0.408323930706876 1384 Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress translational initiation translation initiation factor activity ribosome|multi-eIF complex|eukaryotic translation initiation factor 2 complex|cytoplasm YNL335W_p DDI3 hom 0.014281690282782 0.40825795194714 1385 Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants biological_process molecular_function cellular_component YDR233C RTN1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS 0.0142607592049633 0.408875469924938 1386 Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum organization|endoplasmic reticulum tubular network maintenance|nuclear pore complex assembly molecular_function integral to endoplasmic reticulum membrane|mitochondrion|Golgi apparatus|endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YPL008W CHL1 hom FT CELL CYCLE|CHROMOSOME SEGREGATION|NUCLEUS 0.0142214341482311 0.410238647395524 1387 Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome negative regulation of DNA recombination|chromosome segregation|donor selection|DNA replication initiation|mitotic sister chromatid cohesion DNA helicase activity nucleus YIL046W-A_p YIL046W-A hom 0.0141805594509818 0.411515753900084 1388 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YDR182W CDC1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM 0.0141711583080697 0.411825913021746 1389 Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution DNA repair|cellular manganese ion homeostasis|dephosphorylation|actin cytoskeleton organization molecular_function integral to membrane|endoplasmic reticulum YOR198C BFR1 hom FT CELL CYCLE|ENDOMEMBRANE SYSTEM 0.0141702387837242 0.41185625707773 1390 Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity meiosis|mRNA metabolic process|regulation of mitosis RNA binding ribonucleoprotein complex|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YJL015C_d YJL015C het 0.0141663609056189 0.412193444641713 1391 Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Unknown Unknown Unknown YOR018W ROD1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0141614187918864 0.412147381769668 1392 Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication response to drug|ubiquitin-dependent endocytosis ubiquitin protein ligase binding plasma membrane YLR041W_d YLR041W hom 0.0141574822092688 0.412277357348479 1393 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Unknown Unknown Unknown YHL014C YLF2 hom FT MITOCHONDRION 0.0141570351172527 0.412292120683995 1394 Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process GTP binding mitochondrion YDR217C RAD9 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0141231300361174 0.413412606044829 1395 DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate DNA repair|mitotic cell cycle G1/S transition DNA damage checkpoint|regulation of cell cycle|intra-S DNA damage checkpoint|nucleotide-excision repair|DNA damage checkpoint|positive regulation of transcription from RNA polymerase II promoter double-stranded DNA binding|histone binding chromatin|nucleus YLR194C YLR194C hom FT CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.0141201690646229 0.413858930032759 1396 Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress fungal-type cell wall organization structural constituent of cell wall fungal-type cell wall|anchored to plasma membrane YLR231C BNA5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.0141035078839675 0.414061895809999 1397 Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p de novo NAD biosynthetic process from tryptophan kynureninase activity nucleus|cytoplasm YHR020W YHR020W het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0140948811206001 0.41469572021352 1398 Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments chromosome organization|tRNA aminoacylation for protein translation proline-tRNA ligase activity ribosome YCR043C_p YCR043C hom FT GOLGI APPARATUS|SITE OF POLARIZED GROWTH 0.014086037057373 0.414640506223951 1399 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene biological_process molecular_function Golgi apparatus|mating projection tip YLL060C GTT2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.0140788954783458 0.414877163297272 1400 Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress glutathione metabolic process glutathione transferase activity mitochondrion YJL079C PRY1 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0140542964851585 0.415762466906775 1401 Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication biological_process molecular_function nuclear envelope|extracellular region|endoplasmic reticulum|fungal-type vacuole YJL057C IKS1 hom FT PROTEIN PHOSPHORYLATION 0.0140354085356252 0.416319951810416 1402 Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p protein phosphorylation protein kinase activity cellular_component YHL021C AIM17 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0140317997345366 0.416648354624116 1403 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YER078C ICP55 hom FT PROTEOLYSIS|NUCLEUS|MITOCHONDRION 0.0140284203197083 0.416760600967491 1404 Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family protein stabilization|protein processing aminopeptidase activity extrinsic to mitochondrial inner membrane|mitochondrion|nucleus YJL059W YHC3 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.0140253267793515 0.416654861509559 1405 Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) basic amino acid transport|regulation of intracellular pH|arginine transport molecular_function integral to membrane|fungal-type vacuole YDR316W OMS1 hom FT MITOCHONDRION 0.0140148955188996 0.417071015919286 1406 Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations biological_process S-adenosylmethionine-dependent methyltransferase activity mitochondrial envelope|mitochondrion YIL095W PRK1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0140046945545998 0.417340746244596 1407 Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|actin cortical patch assembly|cytokinesis protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YDR288W NSE3 het FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0139927849881213 0.418292881310442 1408 Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress DNA repair DNA binding Smc5-Smc6 complex|nucleus|cytoplasm YOR014W RTS1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|SIGNALING|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON 0.0139844694777666 0.418083156733697 1409 B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A mitotic cell cycle spindle orientation checkpoint|cellular protein localization|meiotic sister chromatid cohesion, centromeric|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|spindle pole body|condensed nuclear chromosome, centromeric region|cellular bud neck|cytoplasm YNL143C_p YNL143C hom 0.0139817577031309 0.418381771156485 1410 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YLR165C PUS5 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|MITOCHONDRION 0.0139719645072988 0.418430166999844 1411 Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability rRNA modification|pseudouridine synthesis pseudouridylate synthase activity mitochondrion YGL097W SRM1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|CELL DIVISION|RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.0139662151915802 0.418829909829114 1412 Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p ribosomal subunit export from nucleus|rRNA export from nucleus signal transducer activity nuclear chromatin|integral to membrane|nucleus YLR044C PDC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.0139506754457075 0.419139671461741 1413 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism L-phenylalanine catabolic process|pyruvate metabolic process|glucose catabolic process to ethanol|tryptophan catabolic process|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway pyruvate decarboxylase activity cytosol|nucleus|cytoplasm YDL179W PCL9 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.0139333729301967 0.419716836267564 1414 Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p regulation of establishment or maintenance of cell polarity|regulation of transcription involved in G1/S phase of mitotic cell cycle cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|incipient cellular bud site|cellular bud neck YKL159C RCN1 hom FT SIGNALING 0.0138734770806949 0.421718401437861 1415 Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region calcium-mediated signaling protein phosphatase 2B binding|calcium-dependent protein serine/threonine phosphatase regulator activity cytoplasm YBR114W RAD16 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.0138533192031771 0.422393282138599 1416 Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex nucleotide-excision repair, DNA damage recognition|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|damaged DNA binding|DNA-dependent ATPase activity nucleotide-excision repair factor 4 complex|Cul3-RING ubiquitin ligase complex YLR404W FLD1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0138501804828335 0.422498422597154 1417 Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy lipid particle organization molecular_function integral to membrane|endoplasmic reticulum|cortical endoplasmic reticulum YML064C TEM1 het FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON 0.0138447871724494 0.422679122818727 1418 GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis regulation of exit from mitosis protein binding|GTPase activity spindle pole body YPR070W MED1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0138383080138703 0.42289626367341 1419 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YPL249C GYP5 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0138111083296765 0.424085084756983 1420 GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress vesicle-mediated transport|ER to Golgi vesicle-mediated transport Rab GTPase activator activity cytosol|incipient cellular bud site|plasma membrane|cellular bud neck|Golgi-associated vesicle|cellular bud tip YMR018W_p YMR018W hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0138004694343285 0.424165679634577 1421 Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene biological_process molecular_function cellular_component YMR264W CUE1 hom FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|UBIQUITIN LIGASE COMPLEX 0.0137946990580534 0.424359460854871 1422 Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication ER-associated protein catabolic process|establishment of protein localization to endoplasmic reticulum membrane ubiquitin-protein ligase activator activity Doa10p ubiquitin ligase complex|mitochondrion|integral to endoplasmic reticulum membrane YGL207W SPT16 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0137911837202707 0.424477538551678 1423 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome DNA replication-independent nucleosome organization|regulation of transcription by chromatin organization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription initiation from RNA polymerase II promoter|nucleosome assembly|transcription elongation from RNA polymerase II promoter|DNA-dependent DNA replication chromatin binding|nucleosome binding|histone binding nuclear chromatin|FACT complex|replication fork protection complex|alpha DNA polymerase:primase complex|transcription elongation factor complex YDL087C LUC7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0137846962994202 0.424695496799191 1424 Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA mRNA splice site selection mRNA binding U1 snRNP|U2-type prespliceosome YDR234W LYS4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.013750827282464 0.425834457126892 1425 Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway lysine biosynthetic process via aminoadipic acid homoaconitate hydratase activity mitochondrion YDR393W SHE9 hom FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0137452288637532 0.426022894356662 1426 Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex mitochondrion organization|inner mitochondrial membrane organization molecular_function mitochondrial inner membrane YLR097C HRT3 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.0137389796581502 0.426233293856137 1427 Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YOR362C PRE10 het FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0137302429650658 0.426527544114174 1428 Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process mRNA binding mitochondrion|proteasome core complex, alpha-subunit complex|nucleus|proteasome storage granule|nuclear outer membrane-endoplasmic reticulum membrane network YFL049W SWP82 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|RSC COMPLEX 0.0137277443327051 0.426680678195879 1429 Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YDL071C_d YDL071C hom 0.013716464194002 0.42726770355178 1430 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Unknown Unknown Unknown YGL147C RPL9A hom FT TRANSLATION|RIBOSOME 0.0136942990994118 0.427739371953891 1431 Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YPR043W RPL43A hom FT TRANSLATION|RIBOSOME 0.0136785882108069 0.428269684724512 1432 Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR494W RSM28 hom FT TRANSLATION|MITOCHONDRION|RIBOSOME 0.0136747189085928 0.428400349648173 1433 Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation cytoplasmic translation|translational initiation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YIR038C GTT1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0136715770198406 0.428575321240918 1434 ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p glutathione metabolic process glutathione transferase activity|glutathione peroxidase activity mitochondrial outer membrane|mitochondrion|endoplasmic reticulum|plasma membrane YOR345C_d YOR345C hom 0.0136707349011777 0.428534912346658 1435 Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YFR036W CDC26 hom FF|FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.013665614881622 0.428707880749402 1436 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YPL155C KIP2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.0136477751426389 0.42931087339071 1437 Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle nuclear migration along microtubule|mitotic anaphase B|negative regulation of microtubule depolymerization microtubule motor activity mitochondrion|kinesin complex|cytoplasmic microtubule YMR060C SAM37 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0136439230110675 0.429441142131742 1438 Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion|integral to membrane YIL117C PRM5 hom 0.0136367725582254 0.429683012090888 1439 Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling biological_process molecular_function integral to membrane YGR070W ROM1 hom FT SIGNALING|NUCLEOTIDE METABOLISM 0.013622650472962 0.430504909386183 1440 GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP small GTPase mediated signal transduction|activation of Rho GTPase activity Rho guanyl-nucleotide exchange factor activity intracellular YPL206C PGC1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0135998763895173 0.430932311892359 1441 Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs glycerophospholipid catabolic process|phosphatidylglycerol catabolic process phosphatidylglycerol phospholipase C activity mitochondrion|lipid particle YIR024C YIR024C hom FT MITOCHONDRION 0.0135962707678053 0.431054510663752 1442 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect biological_process molecular_function mitochondrion YNL144C_p YNL144C hom FT MITOCHONDRION 0.0135953803933277 0.431084689597155 1443 Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study biological_process molecular_function mitochondrion YPL209C IPL1 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0135863192443451 0.431391883742759 1444 Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest spindle checkpoint|attachment of spindle microtubules to kinetochore|chromosome segregation|mitotic spindle disassembly|protein phosphorylation|meiotic sister chromatid segregation|homologous chromosome segregation|regulation of cytokinesis|mitotic cell cycle G2/M transition decatenation checkpoint protein kinase activity spindle microtubule|spindle midzone|chromosome passenger complex|condensed nuclear chromosome kinetochore|kinetochore microtubule|spindle YFL010W-A AUA1 hom 0.0135845642256225 0.43165751277356 1445 Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease amino acid transport molecular_function cellular_component YDR362C TFC6 het FT NUCLEUS 0.0135806577662056 0.431927455942241 1446 One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity transcription factor TFIIIC complex YCL049C_p YCL049C hom 0.0135653188155197 0.432104334893426 1447 Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene biological_process molecular_function membrane YGR127W_p YGR127W hom 0.0135375374653758 0.433047878473376 1448 Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component YPR052C NHP6A hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.013523904143725 0.4335113452192 1449 High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress mismatch repair|DNA replication-independent nucleosome organization|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly|chromatin remodeling nucleosome binding|sequence-specific DNA binding|DNA binding, bending MutSalpha complex|nucleus YJL093C TOK1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0135228029263927 0.433548793772359 1450 Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin cellular potassium ion homeostasis potassium channel activity integral to membrane|plasma membrane YFL031W HAC1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|NUCLEUS 0.0134969639290835 0.434428024270485 1451 Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress endoplasmic reticulum unfolded protein response|positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response|negative regulation of transcription from RNA polymerase II promoter during meiosis|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YBR245C ISW1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.0134940979914015 0.434525607686808 1452 ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions termination of RNA polymerase I transcription|nucleosome positioning|termination of RNA polymerase II transcription|regulation of transcription by chromatin organization|DNA-dependent transcription, elongation|regulation of transcriptional start site selection at RNA polymerase II promoter|heterochromatin maintenance involved in chromatin silencing|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter nucleosome binding|ATPase activity|rDNA binding|DNA binding ISW1 complex|nucleolar chromatin YPR031W NTO1 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0134743389909712 0.435198733788547 1453 Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 histone acetylation histone acetyltransferase activity|methylated histone residue binding nucleus|NuA3 histone acetyltransferase complex|cytoplasm YGR169C-A_p YGR169C-A hom 0.0134734061871775 0.435230526298789 1454 Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YLR096W KIN2 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION|PLASMA MEMBRANE 0.0134477512495063 0.43610544185356 1455 Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p protein autophosphorylation|exocytosis|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity plasma membrane YER009W NTF2 het FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.0134343528456653 0.436562772886065 1456 Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport nucleocytoplasmic transport|protein import into nucleus Ran GTPase binding nuclear envelope|integral to membrane YML006C GIS4 hom FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0134296542830413 0.436723215472469 1457 CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway cellular ion homeostasis|intracellular signal transduction molecular_function plasma membrane YDR115W YDR115W hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.0134224479968987 0.436969355658217 1458 Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrial large ribosomal subunit YNL088W TOP2 het FT CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0134041890176082 0.437593372479791 1459 Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation chromatin assembly or disassembly|chromatin remodeling at centromere|replication fork progression beyond termination site|mitotic cell cycle G2/M transition decatenation checkpoint|DNA topological change|DNA strand elongation involved in DNA replication|regulation of mitotic recombination|reciprocal meiotic recombination DNA topoisomerase (ATP-hydrolyzing) activity DNA replication termination region|synaptonemal complex|mitochondrion|nucleus YDR163W CWC15 hom FT RNA PROCESSING|NUCLEUS 0.0134021386178579 0.437663478706908 1460 Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YGL007W_d BRP1 hom FF 0.013367823865389 0.438837709576747 1461 Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Unknown Unknown Unknown YLR366W_d YLR366W hom 0.0133240938695547 0.440404911151879 1462 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Unknown Unknown Unknown YGR228W_d YGR228W hom 0.0133145370631024 0.440664734477353 1463 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Unknown Unknown Unknown YPR074C TKL1 hom FF|FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.0132963436330263 0.441289522613321 1464 Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication pentose-phosphate shunt transketolase activity cytoplasm YHR022C_p YHR022C hom FT SIGNALING 0.0132931354955676 0.441399747176413 1465 Putative protein of unknown function; YHR022C is not an essential gene biological_process molecular_function cellular_component YBL046W PSY4 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0132697568463998 0.442203462065617 1466 Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Unknown|protein dephosphorylation protein phosphatase regulator activity nucleus|cytoplasm YDR151C CTH1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|NUCLEUS 0.0132585391558529 0.442589402569062 1467 Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis nuclear-transcribed mRNA catabolic process|cellular iron ion homeostasis mRNA binding nucleus YIR005W IST3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RNA LOCALIZATION|NUCLEUS 0.0132483193748107 0.442941177932177 1468 Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing mRNA export from nucleus|spliceosomal complex assembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step first spliceosomal transesterification activity RES complex|U2 snRNP YDR077W SED1 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0132337580840521 0.443442668408773 1469 Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites fungal-type cell wall organization|mitochondrial genome maintenance structural constituent of cell wall mitochondrion|ribosome|fungal-type cell wall YDR182W-A_p YDR182W-A hom 0.0132309155115375 0.443540604346027 1470 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGL258W VEL1 hom 0.0132044345114881 0.444453554822574 1471 Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C biological_process molecular_function cytosol YJL128C PBS2 hom FF|FT PROTEIN LOCALIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH 0.013162486528626 0.445901931423183 1472 MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition activation of MAPK activity involved in osmosensory signaling pathway|actin filament organization|MAPK import into nucleus involved in osmosensory signaling pathway|protein phosphorylation|hyperosmotic response|osmosensory signaling pathway|cellular response to heat MAP-kinase scaffold activity|MAP kinase kinase activity|protein kinase activity cellular bud neck|cytoplasm|cellular bud tip YKR055W RHO4 hom FT SIGNALING|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0131572974538134 0.446081285970734 1473 Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity positive regulation of formin-nucleated actin cable assembly|maintenance of cell polarity|regulation of formin-nucleated actin cable assembly GTPase activity incipient cellular bud site|plasma membrane|cellular bud neck YDR083W RRP8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0131572510589268 0.446082889743124 1474 Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 rRNA processing methyltransferase activity nucleolus YER073W ALD5 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0131475633778846 0.446417844125856 1475 Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity mitochondrion YJL052W TDH1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0131214314964588 0.447457564407971 1476 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress gluconeogenesis|glycolysis glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity mitochondrion|fungal-type cell wall|lipid particle|plasma membrane|cytoplasm YMR001C-A_p YMR001C-A hom 0.0130858787610962 0.44855396129632 1477 Putative protein of unknown function biological_process molecular_function cellular_component YJR012C_p YJR012C het 0.0130617070735877 0.449392597784617 1478 Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W biological_process molecular_function cellular_component YJR042W NUP85 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.0130526197738117 0.449708111323921 1479 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 positive regulation of transcription, DNA-dependent|mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore distribution structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore outer ring YNR024W MPP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0130481597751148 0.450065783541536 1480 Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes nuclear mRNA surveillance of spliceosomal pre-mRNA splicing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding ribosome|nucleus|nuclear exosome (RNase complex)|TRAMP complex YKL065C YET1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0130313645916563 0.450446587049097 1481 Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function ribosome|integral to membrane|endoplasmic reticulum|endoplasmic reticulum membrane YDL173W PAR32 hom 0.0130312684388881 0.450449929275268 1482 Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene biological_process molecular_function cytoplasm YHR153C SPO16 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.0129925513092453 0.453895198048002 1483 Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation synaptonemal complex assembly|ascospore formation|regulation of reciprocal meiotic recombination molecular_function condensed nuclear chromosome YDR208W MSS4 het FT LIPID METABOLISM|NUCLEUS|PLASMA MEMBRANE 0.0129908048836628 0.451857669505031 1484 Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation phosphatidylinositol phosphorylation|ascospore-type prospore assembly 1-phosphatidylinositol-4-phosphate 5-kinase activity nucleus|plasma membrane YEL015W EDC3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.01298978845192 0.451960508290877 1485 Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress deadenylation-independent decapping of nuclear-transcribed mRNA|cytoplasmic mRNA processing body assembly molecular_function cytoplasmic mRNA processing body YFR019W FAB1 hom FT LIPID METABOLISM|MITOCHONDRION 0.0129868833425873 0.452264080777572 1486 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis phosphatidylinositol phosphorylation 1-phosphatidylinositol-3-phosphate 5-kinase activity|phosphatidylinositol-3-phosphate binding endosome membrane|mitochondrion|fungal-type vacuole membrane|PAS complex YHR086W-A_p YHR086W-A hom 0.0129864361096302 0.452009809149188 1487 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGR151C_d YGR151C hom 0.0129592884121534 0.453158032645879 1488 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Unknown Unknown Unknown YOR001W RRP6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS 0.0129544784330082 0.45346057520981 1489 Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|histone mRNA catabolic process|nuclear polyadenylation-dependent antisense transcript catabolic process|U1 snRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear retention of pre-mRNA at the site of transcription|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription 3'-5'-exoribonuclease activity nucleolus|nucleus|nuclear exosome (RNase complex) YKL142W MRP8 hom 0.0129305589861356 0.453958243511509 1490 Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis biological_process molecular_function cytoplasm YGR237C_p YGR237C hom 0.0129293550925193 0.454269599548027 1491 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YOR220W RCN2 hom 0.0129292730742559 0.454070446265275 1492 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm YNL269W BSC4 hom 0.0129237504338016 0.454195980769054 1493 Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p biological_process molecular_function cellular_component YLR357W RSC2 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|RSC COMPLEX 0.0129191372095448 0.454357102642209 1494 Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance ATP-dependent chromatin remodeling|chromosome segregation|nucleosome disassembly|plasmid maintenance|sister chromatid cohesion|double-strand break repair via nonhomologous end joining|transcription elongation from RNA polymerase II promoter|double-strand break repair via homologous recombination DNA translocase activity RSC complex YJL210W PEX2 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.0128947674206933 0.455208778174699 1495 RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import protein import into peroxisome matrix ubiquitin-protein ligase activity|protein binding peroxisomal membrane YIL088C AVT7 hom FT PLASMA MEMBRANE 0.0128860247702014 0.455716205763877 1496 Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters amino acid transport amino acid transmembrane transporter activity integral to membrane|plasma membrane|fungal-type vacuole YLR312C_p YLR312C hom 0.0128851437582872 0.455545353285953 1497 Putative protein of unknown function biological_process molecular_function cellular_component YPL233W NSL1 het FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.012851521558188 0.456722344420796 1498 Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YDR078C SHU2 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.0128492837466198 0.457472669420309 1499 Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function recombinational repair|maintenance of rDNA|error-free translesion synthesis molecular_function Shu complex YEL039C CYC7 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0128471588631258 0.457076591237822 1500 Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication mitochondrial electron transport, cytochrome c to oxygen|mitochondrial electron transport, ubiquinol to cytochrome c electron carrier activity mitochondrion|mitochondrial intermembrane space YBL039C URA7 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0128337817996376 0.457679624028992 1501 Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication pyrimidine nucleobase biosynthetic process|CTP biosynthetic process|phospholipid biosynthetic process CTP synthase activity cytosol YML090W_d YML090W hom 0.0128296765675134 0.45748797277332 1502 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Unknown Unknown Unknown YOR233W KIN4 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0127778721165766 0.459641431460715 1503 Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication mitotic cell cycle spindle orientation checkpoint|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity cell cortex|spindle pole body|cellular bud neck YCL008C STP22 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.0127747921781084 0.459414748206327 1504 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype protein targeting to membrane|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT I complex|internal side of plasma membrane YOR324C FRT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0127684736749092 0.459636857270609 1505 Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication response to stress molecular_function endoplasmic reticulum YJL177W RPL17B hom FT TRANSLATION|RIBOSOME 0.0127654669514866 0.459742571206297 1506 Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR056C_p YDR056C hom FT ENDOPLASMIC RETICULUM 0.0127440465154543 0.460496089812293 1507 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein biological_process molecular_function endoplasmic reticulum YPL259C APM1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0127351425759992 0.460809511676185 1508 Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YBR193C MED8 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0127316694496001 0.460931799282277 1509 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II transcription corepressor activity|structural molecule activity core mediator complex YIL168W YIL168W hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0126664914867005 0.463363442995226 1510 Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase serine family amino acid metabolic process L-serine ammonia-lyase activity cellular_component YPL035C_d YPL035C hom 0.012631737339993 0.464791295519594 1511 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Unknown Unknown Unknown YJR093C FIP1 het FT RNA PROCESSING|NUCLEUS 0.0126262439125909 0.464652413849065 1512 Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p mRNA cleavage|mRNA polyadenylation RNA binding|protein binding, bridging mRNA cleavage and polyadenylation specificity factor complex YLR083C EMP70 hom FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.0126164022618846 0.465000589693417 1513 Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments cellular copper ion homeostasis|vacuolar transport|pseudohyphal growth|endosomal transport|invasive growth in response to glucose limitation molecular_function endosome|fungal-type vacuole membrane|integral to membrane YFL002C SPB4 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0126152553471971 0.465041174431813 1514 Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients ribosomal large subunit assembly|rRNA processing ATP-dependent RNA helicase activity nucleolus|90S preribosome YGL226C-A OST5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0126049455562792 0.465406085207972 1515 Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YGR206W MVB12 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|VACUOLAR TRANSPORT 0.0125931324039422 0.465824402324595 1516 ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin negative regulation of protein complex assembly|protein targeting to vacuole|endosome transport via multivesicular body sorting pathway ubiquitin binding cytosol|endosome|ESCRT I complex YDR363W-A SEM1 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|PROTEOLYSIS|RNA LOCALIZATION|NUCLEUS 0.0125899048074628 0.4659387314761 1517 Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|regulation of cell cycle|mRNA export from nucleus|exocytosis|filamentous growth|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|proteasome storage granule|proteasome regulatory particle, lid subcomplex YER119C-A_d YER119C-A hom FF 0.0125787845883515 0.466332755381274 1518 Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Unknown Unknown Unknown YLR052W IES3 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.012574765547332 0.466608146537827 1519 Subunit of the INO80 chromatin remodeling complex nucleosome mobilization|telomere maintenance|chromatin silencing at telomere molecular_function nucleus|Ino80 complex YLR073C RFU1 hom 0.0125514782067875 0.467301089272902 1520 Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes regulation of ubiquitin homeostasis enzyme inhibitor activity endosome YER121W_p YER121W hom 0.0125436373621674 0.467844972999616 1521 Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy biological_process molecular_function cellular_component YHR167W THP2 hom FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION|NUCLEUS 0.0124912910238723 0.469439365706029 1522 Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance mRNA export from nucleus|transcription elongation from RNA polymerase II promoter|DNA recombination nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YBR154C RPB5 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0124763978903513 0.470632134011431 1523 RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YER170W ADK2 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION 0.0124542966364874 0.471087290762722 1524 Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background nucleotide metabolic process nucleoside triphosphate adenylate kinase activity|adenylate kinase activity mitochondrion|mitochondrial inner membrane YOR357C SNX3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0124525472493759 0.470818675568971 1525 Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p protein localization|late endosome to Golgi transport phosphatidylinositol-3-phosphate binding cytosol|endosome YMR199W CLN1 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0124165173774112 0.472169433888338 1526 G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity nucleus|cytoplasm YDR373W FRQ1 het FT SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.0124061544667657 0.472473233542735 1527 N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily regulation of signal transduction enzyme activator activity|calcium ion binding Golgi membrane YDR522C SPS2 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|PLASMA MEMBRANE 0.012373983467391 0.473622460773261 1528 Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component ascospore wall assembly|ascospore formation molecular_function fungal-type cell wall|plasma membrane YGR084C MRP13 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0123495661171189 0.474957265273184 1529 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YGR035C_p YGR035C hom 0.0123467440657769 0.474596719168214 1530 Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function cellular_component YKR046C PET10 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0123462936388055 0.474612838610922 1531 Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange aerobic respiration molecular_function lipid particle YKL044W_p YKL044W hom 0.0123457027719647 0.474633984434875 1532 Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YNL278W CAF120 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|SITE OF POLARIZED GROWTH 0.0123375031568578 0.474927483992833 1533 Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter molecular_function CCR4-NOT complex YOR115C TRS33 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0123307810680931 0.475168170781478 1534 One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport|protein complex assembly molecular_function trans-Golgi network|TRAPP complex|cis-Golgi network YDR239C YDR239C hom 0.0123267322877288 0.475313171087914 1535 Protein of unknown function that may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome|cytoplasm YGL255W ZRT1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.0123248900716973 0.475708423601148 1536 High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor high-affinity zinc ion transport high affinity zinc uptake transmembrane transporter activity integral to membrane|integral to plasma membrane YOR364W_d YOR364W hom 0.0123052744851475 0.476082051402648 1537 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Unknown Unknown Unknown YNL272C SEC2 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SITE OF POLARIZED GROWTH 0.0122602151015549 0.477698843869325 1538 Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles autophagy|exocytosis guanyl-nucleotide exchange factor activity|phosphatidylinositol-4-phosphate binding cytosol|transport vesicle|mating projection tip|cellular bud neck|cellular bud tip YBR230C OM14 hom FT MITOCHONDRION 0.0122579891103678 0.477778793219057 1539 Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron biological_process molecular_function mitochondrion|integral to mitochondrial outer membrane YBL026W LSM2 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0122510572809305 0.478027805677105 1540 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YMR299C DYN3 hom FT CYTOSKELETON 0.0122288938594333 0.478824459066413 1541 Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration nuclear migration along microtubule motor activity cytoplasmic dynein complex|cytoplasmic microtubule YLR035C MLH2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.0121824245338231 0.480497126566108 1542 Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p mismatch repair|reciprocal meiotic recombination molecular_function nucleus YOR101W RAS1 hom FT SIGNALING|NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.0121811890370693 0.480607083593308 1543 GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress adenylate cyclase-activating G-protein coupled receptor signaling pathway|replicative cell aging|Ras protein signal transduction GTPase activity plasma membrane YDR091C RLI1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM|NUCLEUS|RIBOSOME 0.0121782309176785 0.480648232559446 1544 Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species translational termination|ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|translational initiation ATPase activity|iron ion binding cytosolic ribosome|nucleus|cytoplasm YGR126W_p YGR126W hom FT NUCLEUS 0.0121717978920042 0.480880080106557 1545 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YKL041W VPS24 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM 0.0121616371018375 0.481246401030923 1546 One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YPL086C ELP3 hom FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION|NUCLEUS 0.0121586672160857 0.481353501225273 1547 Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification histone acetyltransferase activity Elongator holoenzyme complex|nucleus|cytoplasm YMR052W FAR3 hom FT CELL CYCLE|SIGNALING|ENDOPLASMIC RETICULUM 0.0121551006323637 0.48148213671221 1548 Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress re-entry into mitotic cell cycle after pheromone arrest molecular_function endoplasmic reticulum YMR020W FMS1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM 0.012152102007379 0.481590302177177 1549 Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis pantothenate biosynthetic process|polyamine catabolic process polyamine oxidase activity cytoplasm YOL005C RPB11 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0121360190248155 0.482235987299362 1550 RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase II, core complex YFR055W_p IRC7 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.012130653558266 0.48236436914933 1551 Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner sulfur compound metabolic process|L-cysteine catabolic process to pyruvate cysteine-S-conjugate beta-lyase activity cellular_component YJL190C RPS22A hom FT TRANSLATION|RIBOSOME 0.012097245279283 0.483571407426238 1552 Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YPL091W GLR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|MITOCHONDRION 0.0120935073998999 0.483706558527212 1553 Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|protein glutathionylation glutathione-disulfide reductase activity mitochondrion|nucleus|cytoplasm YML049C RSE1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0120860170087539 0.484042633859315 1554 Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport spliceosomal complex assembly|mRNA splicing, via spliceosome U2 snRNA binding U2 snRNP|U2-type prespliceosome YNR062C_p YNR062C hom 0.0120724310886025 0.484469001346881 1555 Putative membrane protein of unknown function biological_process molecular_function integral to membrane YBR297W MAL33 hom FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM|NUCLEUS 0.0120440382518693 0.485497150550477 1556 MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C cellular carbohydrate metabolic process|regulation of transcription, DNA-dependent sequence-specific DNA binding transcription factor activity nucleus YBL050W SEC17 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION 0.0120100667836901 0.486728860364762 1557 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP autophagy|SNARE complex disassembly|vesicle fusion with Golgi apparatus|vacuole fusion, non-autophagic ATPase activator activity|soluble NSF attachment protein activity SNARE complex|cytosol|extrinsic to membrane YER014W HEM14 hom FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM|MITOCHONDRION 0.0120092924713693 0.486756954410662 1558 Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides heme biosynthetic process oxygen-dependent protoporphyrinogen oxidase activity mitochondrion|mitochondrial inner membrane YJR064W CCT5 het 0.011981223007203 0.487970694146685 1559 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YKL101W HSL1 hom FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0119703213569943 0.48817205755148 1560 Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p G2/M transition of mitotic cell cycle|regulation of cell cycle|cell shape checkpoint|protein autophosphorylation|protein phosphorylation|septin checkpoint protein kinase activity cellular bud neck|septin ring YBR107C IML3 hom FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.0119686705445995 0.488232050098648 1561 Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 establishment of meiotic sister chromatid cohesion|ascospore formation|protein localization to chromosome, centromeric region|meiotic sister chromatid segregation|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome outer kinetochore YHR076W PTC7 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0119663141728091 0.488317690424172 1562 Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope biological_process protein serine/threonine phosphatase activity nuclear envelope|mitochondrion YER169W RPH1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS 0.0119566336015296 0.488669607483282 1563 JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|histone demethylation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|histone demethylase activity (H3-K36 specific)|histone demethylase activity (H3-K9 specific) nucleus YDL212W SHR3 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH 0.0119434476125732 0.489149176435286 1564 Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface protein folding|ER to Golgi vesicle-mediated transport unfolded protein binding integral to endoplasmic reticulum membrane|integral to membrane|mating projection tip YGR259C_d YGR259C hom 0.0119055524073882 0.490722892044674 1565 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Unknown Unknown Unknown YOL117W RRI2 hom FT SIGNALING|NUCLEUS 0.0118451874521249 0.492795327183588 1566 Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YMR007W_d YMR007W hom 0.0118427770746234 0.492818859407525 1567 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL108C PRM10 hom 0.0118320915545469 0.493209236207757 1568 Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function integral to membrane YOR259C RPT4 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.0118253808379152 0.493518931718741 1569 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization ER-associated protein catabolic process|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription elongation from RNA polymerase II promoter|nucleotide-excision repair|proteasome regulatory particle assembly ATPase activity|protein domain specific binding proteasome regulatory particle, base subcomplex|nucleus YNL162W-A_p YNL162W-A hom FT NUCLEUS 0.0118234326804885 0.493783542986185 1570 Putative protein of unknown function; identified by homology biological_process molecular_function nucleus|cytoplasm YPL246C RBD2 hom FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0118166008826823 0.493775454677931 1571 Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p biological_process molecular_function COPI-coated vesicle|integral to membrane|Golgi apparatus YDR008C_d YDR008C hom FF 0.0118127423910741 0.494819570240171 1572 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL076C_d PSY1 hom 0.0118092707810006 0.494043507341074 1573 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Unknown Unknown Unknown YDR113C PDS1 het FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CYTOSKELETON 0.0117903181865396 0.494994404150844 1574 Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation protein localization|recombinational repair|meiosis I|mitotic sister chromatid segregation enzyme binding nucleus|cytoplasm|spindle YGL090W LIF1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0117886325755395 0.494798638550464 1575 Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein double-strand break repair via nonhomologous end joining chromatin binding DNA ligase IV complex|nuclear chromatin|nucleus YGL127C SOH1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|NUCLEUS|RNA POL II, HOLOENZYME 0.0117822194398993 0.49503341425226 1576 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits DNA repair|meiotic gene conversion|meiotic sister chromatid segregation|transcription from RNA polymerase II promoter molecular_function core mediator complex|nucleus YIL142W CCT2 het 0.0117781932279318 0.495180838494124 1577 Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YKL009W MRT4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0117587236246075 0.495958322823218 1578 Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus nuclear-transcribed mRNA catabolic process|ribosomal large subunit assembly|ribosomal large subunit biogenesis|rRNA processing LSU rRNA binding nucleoplasm|nucleolus|nucleus|preribosome, large subunit precursor YPR197C_d YPR197C hom FF 0.0117521435053337 0.496135244377095 1579 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL124C LSM1 hom FF|FT RNA PROCESSING|NUCLEUS 0.0117146712639539 0.498087473740917 1580 Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay RNA binding|RNA cap binding mRNA cap binding complex|cytoplasmic mRNA processing body|cytoplasm YER156C_p YER156C hom FT NUCLEUS 0.0117036584124756 0.4979142427267 1581 Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 biological_process molecular_function nucleus|cytoplasm YOR109W INP53 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|CYTOSKELETON 0.0116833815597617 0.498659239047746 1582 Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity|phosphatidylinositol-3-phosphatase activity|phosphatidylinositol-4-phosphate phosphatase activity membrane|actin cortical patch|cytoplasm YDR071C PAA1 hom FT CHROMATIN ORGANIZATION 0.0116692175683565 0.499179992561139 1583 Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication chromatin organization diamine N-acetyltransferase activity|aralkylamine N-acetyltransferase activity cytoplasm YDR520C_p URC2 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM|NUCLEUS 0.0116667530214368 0.49952663929011 1584 Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism uracil catabolic process sequence-specific DNA binding nucleus|cytoplasm YDL189W RBS1 hom FT CARBOHYDRATE METABOLISM 0.011628507535002 0.500678344552282 1585 Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain galactose metabolic process molecular_function cytoplasm YHR156C LIN1 hom FT NUCLEUS|CHROMOSOME 0.0116267693889987 0.50074237084217 1586 Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication biological_process protein binding chromatin|nucleus|U4/U6 x U5 tri-snRNP complex|U5 snRNP YKL020C SPT23 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|GENE SILENCING|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0115957764288371 0.502076098661134 1587 ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting cellular response to cold|positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA binding integral to endoplasmic reticulum membrane|nucleus YHR079C IRE1 hom FF|FT RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0115938457651933 0.501955963269733 1588 Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|inositol metabolic process|protein phosphorylation unfolded protein binding|protein serine/threonine kinase activity|endoribonuclease activity|protein kinase activity|protein homodimerization activity nucleus YHR115C DMA1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.0115887825907005 0.502142734061468 1589 Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger establishment of mitotic spindle orientation|protein autoubiquitination|septin ring assembly|mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity cytoplasm YMR120C ADE17 hom FT NUCLEOTIDE METABOLISM 0.0115849323707068 0.502284785883554 1590 Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol|plasma membrane YJL187C SWE1 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.0115701654875407 0.50282979914149 1591 Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate negative regulation of spindle pole body separation|G2/M transition size control checkpoint|protein phosphorylation|re-entry into mitotic cell cycle|G2/M transition of mitotic cell cycle|cell shape checkpoint|regulation of cyclin-dependent protein kinase activity|regulation of cell size|regulation of meiosis protein tyrosine kinase activity|protein kinase activity nucleus|cellular bud neck YGR242W_d YGR242W hom 0.0115619871247441 0.503131778943451 1592 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Unknown Unknown Unknown YMR077C VPS20 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT 0.0115110676990777 0.505204662692676 1593 Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport protein binding ESCRT III complex|cytoplasm YGL221C NIF3 hom FT MITOCHONDRION 0.0114971855543796 0.505527909199547 1594 Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YJR032W CPR7 hom 0.0114969028742812 0.505538374838893 1595 Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity protein refolding|response to stress unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytosol YHR109W CTM1 hom 0.0114931465374645 0.505677456176517 1596 Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth peptidyl-lysine methylation [cytochrome c]-lysine N-methyltransferase activity cytosol YPL097W MSY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.011487917715191 0.50587109096517 1597 Mitochondrial tyrosyl-tRNA synthetase mitochondrial tyrosyl-tRNA aminoacylation tyrosine-tRNA ligase activity mitochondrion YEL045C_d YEL045C hom 0.0114770805245513 0.506272540128912 1598 Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Unknown Unknown Unknown YDR451C YHP1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS|CHROMOSOME 0.0114666687033028 0.506658389756557 1599 Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II regulatory region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nuclear chromatin|nucleus YFR012W DCV1 hom 0.011441620187926 0.507587290398172 1600 Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YBR076W ECM8 hom FT CELL WALL ORG/BIOGENESIS 0.0114408904791988 0.507614364350508 1601 Non-essential protein of unknown function fungal-type cell wall organization molecular_function cellular_component YOL021C DIS3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0114296408203851 0.508095108040613 1602 Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress nuclear mRNA surveillance|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing 3'-5'-exoribonuclease activity|endoribonuclease activity|tRNA binding mitochondrion|nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YML060W OGG1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.0114102631973778 0.508751393329101 1603 Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance DNA repair|base-excision repair, AP site formation oxidized purine nucleobase lesion DNA N-glycosylase activity mitochondrion YDR463W STP1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|PLASMA MEMBRANE 0.011387919580566 0.50989751041206 1604 Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication positive regulation of transcription, DNA-dependent|tRNA splicing, via endonucleolytic cleavage and ligation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription plasma membrane|nucleus|cytoplasm YCR051W_p YCR051W hom FT NUCLEUS 0.0113764137394214 0.510009595177367 1605 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene biological_process molecular_function nucleus|cytoplasm YPL160W CDC60 het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.0113703328394455 0.510235797672694 1606 Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA leucyl-tRNA aminoacylation|DNA replication initiation leucine-tRNA ligase activity cytoplasm YJR038C_d YJR038C hom 0.0113578557616128 0.510700094803416 1607 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR168C UPS2 hom FT LIPID METABOLISM|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.011350565076195 0.510971497989049 1608 Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI cristae formation|Unknown|phosphatidylethanolamine metabolic process|cardiolipin metabolic process molecular_function extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial intermembrane space YNL284C MRPL10 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0113363220023132 0.511627873074436 1609 Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YNR057C BIO4 hom FT KETONE METABOLISM|COFACTOR METABOLISM 0.0113326233381095 0.511639718078894 1610 Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels biotin biosynthetic process dethiobiotin synthase activity cytoplasm YMR292W GOT1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0113214123863161 0.512309243597122 1611 Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition ER to Golgi vesicle-mediated transport|Golgi to endosome transport molecular_function integral to membrane|endoplasmic reticulum|Golgi apparatus|ER to Golgi transport vesicle|Golgi membrane YOL064C MET22 hom FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.0113020530041632 0.512779325534908 1612 Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway methionine biosynthetic process|hyperosmotic salinity response|sulfate assimilation 3'(2'),5'-bisphosphate nucleotidase activity cellular_component YCR020W-B HTL1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|RSC COMPLEX 0.0112993486439096 0.513446321796126 1613 Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance regulation of cell cycle|nucleosome disassembly|response to DNA damage stimulus|chromatin remodeling|transcription elongation from RNA polymerase II promoter chromatin binding RSC complex YMR017W SPO20 hom FT PLASMA MEMBRANE 0.0112936850310687 0.513091498959608 1614 Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog ascospore-type prospore assembly SNAP receptor activity SNARE complex|prospore membrane YCR090C_p YCR090C hom FT NUCLEUS 0.0112378828736613 0.515489159767654 1615 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus|cytoplasm YPR021C AGC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.0112118009968764 0.516151456843906 1616 Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis L-glutamate transport|aspartate transport|cellular nitrogen compound biosynthetic process antiporter activity|L-aspartate transmembrane transporter activity|L-glutamate transmembrane transporter activity|uniporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YCR067C SED4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0111858024365164 0.517374997028199 1617 Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p positive regulation of GTPase activity|regulation of COPII vesicle coating Sar GTPase activator activity integral to endoplasmic reticulum membrane YLR345W_p YLR345W hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY 0.0111802297704628 0.517333776509648 1618 Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YMR311C GLC8 hom FT SIGNALING|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.0111533963372554 0.51833976985936 1619 Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress chromosome segregation|glycogen biosynthetic process enzyme activator activity nucleus|cytoplasm YMR163C INP2 hom FT PEROXISOME ORGANIZATION 0.0111375954957559 0.518994947445749 1620 Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene peroxisome inheritance protein anchor peroxisome|integral to peroxisomal membrane|cytoplasm YGL198W YIP4 hom FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0111091801397085 0.520373250140973 1621 Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport biological_process Rab GTPase binding integral to membrane|Golgi apparatus YEL047C FRD1 hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.011102193609426 0.520262176034485 1622 Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity cytosol|mitochondrion|ribosome|plasma membrane YBR248C HIS7 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0110928758560829 0.520861204988653 1623 Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor purine nucleoside monophosphate biosynthetic process|histidine biosynthetic process imidazoleglycerol-phosphate synthase activity intracellular YDL125C HNT1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0110758403396339 0.521253039445094 1624 Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress nucleotide metabolic process hydrolase activity|nucleotide binding nucleus|cytoplasm YLR295C ATP14 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0110444707890958 0.522433775776165 1625 Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress cristae formation|mitochondrial proton-transporting ATP synthase complex assembly|ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YNL141W AAH1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0110327701564922 0.522874534018192 1626 Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome adenine catabolic process|hypoxanthine salvage|purine-containing compound salvage adenine deaminase activity nucleus|cytoplasm YBR232C_d YBR232C hom 0.0110205356552232 0.523335606923118 1627 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR468C TLG1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|GOLGI APPARATUS 0.0110019797926154 0.524035306248172 1628 Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p endocytosis|vesicle fusion SNAP receptor activity trans-Golgi network|SNARE complex|endosome YHR179W OYE2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS|MITOCHONDRION 0.0109653563529113 0.525417698066937 1629 Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress apoptotic process NADPH dehydrogenase activity mitochondrion|nucleus|cytoplasm YMR194C-A_d YMR194C-A hom 0.0109612122088399 0.52557424068071 1630 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL135C FPR1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION 0.010958819468289 0.525849876391575 1631 Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function protein folding|chromatin organization|homoserine metabolic process peptidyl-prolyl cis-trans isomerase activity|macrolide binding mitochondrion|nucleus|cytoplasm YNL329C PEX6 hom FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION 0.0109442731442311 0.526214351742257 1632 AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol protein import into peroxisome matrix, receptor recycling|replicative cell aging ATPase activity|protein heterodimerization activity cytosol|peroxisome YCL047C POF1 hom FT PROTEOLYSIS 0.0109420371186599 0.526298878751682 1633 ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p pseudohyphal growth|ER-associated protein catabolic process|invasive growth in response to glucose limitation ATPase activity cellular_component YLR225C_p YLR225C hom FT RESPONSE TO OXIDATIVE STRESS 0.0109382855010077 0.526440714270217 1634 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene biological_process molecular_function cytoplasm YGR135W PRE9 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.0109364256269894 0.526511036825399 1635 Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform proteasomal ubiquitin-dependent protein catabolic process|proteasome core complex assembly|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YLR440C SEC39 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.0108915690894411 0.528208531218645 1636 Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope retrograde vesicle-mediated transport, Golgi to ER|vesicle-mediated transport|ER-dependent peroxisome organization molecular_function Dsl1p complex|nuclear envelope|endoplasmic reticulum|peroxisome|endoplasmic reticulum membrane YNL289W PCL1 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0108749625983607 0.529083548709695 1637 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth regulation of establishment or maintenance of cell polarity|G1 phase of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|septin ring organization|positive regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|incipient cellular bud site|nucleus YKL025C PAN3 hom FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION 0.0108635115888788 0.529271718958722 1638 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes DNA repair|mRNA 3'-end processing|postreplication repair poly(A)-specific ribonuclease activity PAN complex|cytoplasm YKL008C LAC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0108500634428363 0.529781697339406 1639 Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p replicative cell aging|ceramide biosynthetic process sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YOR041C_d YOR041C hom 0.0108338406558846 0.530458536405101 1640 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YGR061C ADE6 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM 0.0108175061616167 0.531017362092457 1641 Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway 'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process phosphoribosylformylglycinamidine synthase activity cytoplasm YBR228W SLX1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.0107257104120947 0.53469203181759 1642 Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA-dependent DNA replication 5'-flap endonuclease activity Slx1-Slx4 complex|nucleus YPL254W HFI1 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.0107173328458928 0.534828440020042 1643 Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions histone acetylation|chromatin modification|transcription from RNA polymerase II promoter transcription coactivator activity|transcription cofactor activity SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YJR141W_p YJR141W het 0.0106877091855249 0.535958103676054 1644 Essential protein of unknown function biological_process molecular_function cellular_component YNL111C CYB5 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0106767641708825 0.536558110659936 1645 Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation sterol biosynthetic process electron carrier activity endoplasmic reticulum membrane YOL041C NOP12 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0106762057278443 0.536579420219234 1646 Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA binding nucleolus|preribosome YHR080C YHR080C hom FT MITOCHONDRION 0.0106624017670882 0.536984835702009 1647 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication biological_process molecular_function mitochondrion|ribosome YKR068C BET3 het FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0106399549734148 0.537781679519175 1648 Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex|cis-Golgi network membrane YNL140C_p YNL140C hom 0.0106129620289271 0.538813826864187 1649 Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C biological_process molecular_function cellular_component YKL211C TRP3 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.0106086523654722 0.539948569481495 1650 Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p tryptophan biosynthetic process indole-3-glycerol-phosphate synthase activity|anthranilate synthase activity anthranilate synthase complex|cytoplasm YKL148C SDH1 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.0105945906294053 0.539516873674407 1651 Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits cellular respiration|mitochondrial electron transport, succinate to ubiquinone|tricarboxylic acid cycle succinate dehydrogenase (ubiquinone) activity|flavin adenine dinucleotide binding mitochondrial respiratory chain complex II|mitochondrion|mitochondrial membrane YFL056C AAD6 hom FT OXIDATION-REDUCTION PROCESS 0.0105824789543077 0.539980620930459 1652 Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YKL198C PTK1 hom FT PROTEIN PHOSPHORYLATION 0.0105796926730538 0.540087333823124 1653 Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein polyamine transport protein kinase activity cellular_component YCL055W KAR4 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.0105781381007308 0.540328157145175 1654 Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone karyogamy involved in conjugation with cellular fusion|meiosis|negative regulation of transcription from RNA polymerase II promoter by pheromones|positive regulation of transcription from RNA polymerase II promoter by pheromones|G1 phase of mitotic cell cycle sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YBR259W YBR259W hom 0.0105617547390225 0.540774598308461 1655 Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component YBR196C-B_p YBR196C-B hom 0.0105509673759759 0.541188109887412 1656 Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YKL098W MTC2 hom 0.0105401197218365 0.541905605545542 1657 Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YPR077C_d YPR077C hom 0.0105316788867252 0.541927888104982 1658 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Unknown Unknown Unknown YNL236W SIN4 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0105297888559635 0.542543017120557 1659 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding core mediator complex YHR149C SKG6 hom FT SITE OF POLARIZED GROWTH 0.0105219252153691 0.542302165984822 1660 Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p biological_process molecular_function cell cortex|incipient cellular bud site|integral to membrane|cellular bud neck|cellular bud tip YNL089C_d YNL089C hom 0.0105052068203606 0.542944000912981 1661 Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Unknown Unknown Unknown YLR229C CDC42 het FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.0104864931076548 0.543662886322291 1662 Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins budding cell apical bud growth|Rho protein signal transduction|budding cell isotropic bud growth|regulation of initiation of mating projection growth|regulation of vacuole fusion, non-autophagic|regulation of exit from mitosis|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|establishment of cell polarity|invasive growth in response to glucose limitation|positive regulation of exocytosis GTPase activity|signal transducer activity nuclear membrane|fungal-type vacuole membrane|incipient cellular bud site|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YPL204W HRR25 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION|NUCLEUS|SITE OF POLARIZED GROWTH|P 0.0104754482741953 0.544087394539917 1663 Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) ribosomal small subunit biogenesis|DNA repair|mitosis|ER to Golgi vesicle-mediated transport|protein phosphorylation|attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation|tRNA wobble uridine modification protein serine/threonine kinase activity|protein kinase activity chromosome, centromeric region|plasma membrane|nucleus|spindle pole body|monopolin complex|cellular bud neck|cellular bud tip YPR013C_p CMR3 hom 0.0104750520346177 0.544342874352804 1664 Putative zinc finger protein; YPR013C is not an essential gene biological_process sequence-specific DNA binding cellular_component YAL036C RBG1 hom FT TRANSLATION|RIBOSOME 0.0104572751000118 0.544786238925503 1665 Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid cytoplasmic translation GTP binding polysomal ribosome|polysome|cytoplasm YKL054C DEF1 hom FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS|CHROMOSOME 0.0104502861095651 0.545595139357359 1666 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis transcription-coupled nucleotide-excision repair|telomere maintenance|response to DNA damage stimulus|ubiquitin-dependent protein catabolic process molecular_function nucleus YHR172W SPC97 het FT CYTOSKELETON ORGANIZATION|NUCLEUS|CYTOSKELETON 0.0104322263063658 0.545989837566461 1667 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YDR353W TRR1 het FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.0104201042462252 0.546217020371013 1668 Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication cellular response to oxidative stress|cell redox homeostasis ferrous iron binding|thioredoxin-disulfide reductase activity cytosol YDR378C LSM6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0104079306555148 0.547404403073907 1669 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasmic mRNA processing body YNL032W SIW14 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0104067462696022 0.546731650238789 1670 Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm actin filament organization|endocytosis|protein dephosphorylation|response to stress protein tyrosine phosphatase activity cytoplasm YPL196W OXR1 hom FT RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0103955658701978 0.547162571114105 1671 Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes cellular response to oxidative stress molecular_function mitochondrion YDR500C RPL37B hom FT TRANSLATION|RIBOSOME 0.010391734334621 0.547370026553208 1672 Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YPL239W YAR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS 0.0103908531217271 0.547344262681083 1673 Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock ribosomal small subunit biogenesis|cellular response to oxidative stress|response to osmotic stress molecular_function cytoplasm YOL159C-A YOL159C-A hom FT NUCLEUS 0.01038386990244 0.547971990207312 1674 Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function nucleus|cytoplasm YMR250W GAD1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.0103778030919564 0.547847538817524 1675 Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress cellular response to oxidative stress|glutamate catabolic process calmodulin binding|glutamate decarboxylase activity cytoplasm YHL003C LAG1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0103702740721612 0.548376716707778 1676 Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress replicative cell aging|ceramide biosynthetic process sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YJL164C TPK1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0103665565023354 0.548281448403436 1677 cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication protein phosphorylation|Ras protein signal transduction cAMP-dependent protein kinase activity|protein kinase activity cAMP-dependent protein kinase complex|nucleus|cytoplasm YNR013C PHO91 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.0103440027936046 0.549152113930994 1678 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth phosphate ion transport|polyphosphate metabolic process phosphate ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|membrane YNR049C MSO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.010337102401013 0.549716447064447 1679 Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress cellular membrane fusion|ascospore-type prospore membrane assembly|vesicle docking involved in exocytosis molecular_function SNARE complex|cellular bud membrane|plasma membrane|prospore membrane|cellular bud neck|cellular bud tip YGL100W SEH1 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE|NUCLEAR PORE 0.0103263217180878 0.550013684514528 1680 Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 nucleocytoplasmic transport structural constituent of nuclear pore fungal-type vacuole membrane|integral to membrane|nuclear pore|Seh1-associated complex|nuclear pore outer ring YCR032W BPH1 hom FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.0103209670756326 0.550042090181223 1681 Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting intracellular protein transport|fungal-type cell wall organization|response to pH molecular_function cytosol|mitochondrion|extrinsic to membrane YDR443C SSN2 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0102883348965534 0.551363394159009 1682 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II transcription coactivator activity|RNA polymerase II transcription factor recruiting transcription factor activity mediator complex YHR148W IMP3 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.0102632208100482 0.552276212151532 1683 Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA rRNA processing|ribosome biogenesis snoRNA binding small-subunit processome|Mpp10 complex|90S preribosome YOR043W WHI2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION 0.0102610417305628 0.552360604776522 1684 Protein required, with binding partner Psr1p, for full activation of the general stress response, possibly through Msn2p dephosphorylation; regulates growth during the diauxic shift; negative regulator of G1 cyclin expression response to salt stress|mitochondrion degradation|actin filament organization|endocytosis|response to heat|regulation of growth|response to stress phosphatase activator activity cellular_component YOR134W BAG7 hom FT SIGNALING 0.0102546382145543 0.55260864037921 1685 Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p small GTPase mediated signal transduction Rho GTPase activator activity intracellular YOR121C_d YOR121C hom 0.0102407721735883 0.553441938442427 1686 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Unknown Unknown Unknown YDL040C NAT1 hom FT MITOCHONDRION|RIBOSOME 0.0102289657552985 0.553603592780097 1687 Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity mitochondrion|NatA complex|cytosolic ribosome YKR099W BAS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.0102108558028574 0.560513644723873 1688 Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes positive regulation of transcription from RNA polymerase II promoter core promoter sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity nucleus YKL139W CTK1 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.0102086950651678 0.554389815379771 1689 Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 mRNA 3'-end processing|phosphorylation of RNA polymerase II C-terminal domain|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|peptidyl-serine phosphorylation|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation protein kinase activity|cyclin-dependent protein kinase activity nucleoplasm|nucleolus|nucleus|polysome|carboxy-terminal domain protein kinase complex YGL093W SPC105 het FF|FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0101923295218621 0.555142978148191 1690 Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components microtubule nucleation|protein localization to kinetochore|sister chromatid biorientation|mitotic cell cycle spindle assembly checkpoint microtubule binding|structural constituent of cytoskeleton mitochondrion|condensed nuclear chromosome kinetochore|spindle pole body YDL029W ARP2 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|MITOCHONDRION|CYTOSKELETON 0.010187752723026 0.555202661815271 1691 Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity mitochondrion inheritance|Arp2/3 complex-mediated actin nucleation|ascospore wall assembly|actin filament organization|establishment of mitochondrion localization|ATP catabolic process|CVT pathway ATPase activity|ATP binding Arp2/3 protein complex|mitochondrion YOR193W PEX27 hom FT PEROXISOME ORGANIZATION 0.010161593351584 0.556277698261153 1692 Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p peroxisome organization molecular_function peroxisomal membrane YPL042C SSN3 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.0101569180157028 0.55657715189672 1693 Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression phosphorylation of RNA polymerase II C-terminal domain|negative regulation of transcription from RNA polymerase II promoter|negative regulation of filamentous growth|protein phosphorylation|protein destabilization|nuclear-transcribed mRNA catabolic process, non-stop decay|positive regulation of transcription from RNA polymerase II promoter by galactose RNA polymerase II carboxy-terminal domain kinase activity|protein kinase activity|cyclin-dependent protein kinase activity mediator complex YDL009C_p YDL009C hom 0.0101147919680101 0.55803906455439 1694 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene biological_process molecular_function cellular_component YKR053C YSR3 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0100994515191574 0.558636330758069 1695 Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication phospholipid dephosphorylation|sphingolipid biosynthetic process sphingosine-1-phosphate phosphatase activity integral to membrane|endoplasmic reticulum YOL143C RIB4 hom FT NUCLEUS 0.0100754582809982 0.559629660886494 1696 Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin riboflavin biosynthetic process 6,7-dimethyl-8-ribityllumazine synthase activity nucleus|cytoplasm YER026C CHO1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.0100679938609816 0.559862075816443 1697 Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline phosphatidylserine biosynthetic process CDP-diacylglycerol-serine O-phosphatidyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YJL026W RNR2 het FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|NUCLEUS 0.0100667319997343 0.559969791354907 1698 Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ribonucleoside-diphosphate reductase complex|nucleus|cytoplasm YOR141C ARP8 hom FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.0100627894025722 0.560064992714636 1699 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity mitotic recombination|response to DNA damage stimulus|chromatin remodeling mRNA binding|ATP-dependent 3'-5' DNA helicase activity nucleus|Ino80 complex YMR295C YMR295C hom FT SITE OF POLARIZED GROWTH 0.0100540178690729 0.560465538306003 1700 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|cellular bud|mating projection tip YHR096C HXT5 hom FT PLASMA MEMBRANE 0.0100223353790704 0.561643470579092 1701 Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication glucose transport|hexose transport mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YMR166C_p YMR166C hom FT MITOCHONDRION 0.00997891441814167 0.563572897638831 1702 Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene transport transporter activity integral to membrane|mitochondrial inner membrane YHR041C SRB2 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|NUCLEUS|RNA POL II, HOLOENZYME 0.00994807275834045 0.564546711842033 1703 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus|RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|protein domain specific binding core mediator complex YLL006W MMM1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00992518737795601 0.56555880597029 1704 ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization|mitochondrial genome maintenance|protein import into mitochondrial outer membrane|phospholipid transport molecular_function mitochondrial outer membrane|mitochondrion|integral to endoplasmic reticulum membrane|ERMES complex YPL269W KAR9 hom FT NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00992162119718696 0.565756463708659 1705 Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase nuclear migration along microtubule|establishment of spindle localization molecular_function cell cortex|spindle pole body|mating projection tip YDR069C DOA4 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION 0.00991461626103609 0.565857022802362 1706 Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole protein deubiquitination|endocytosis|regulation of DNA replication|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|free ubiquitin chain depolymerization|ubiquitin homeostasis|ubiquitin-dependent protein catabolic process endopeptidase activity|ubiquitin-specific protease activity|ubiquitin thiolesterase activity endosome|proteasome complex|mitochondrion YPR002W PDH1 hom FT KETONE METABOLISM|MITOCHONDRION 0.00988616480049306 0.566972460576804 1707 Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate propionate metabolic process|propionate catabolic process, 2-methylcitrate cycle 2-methylcitrate dehydratase activity mitochondrial outer membrane|mitochondrion|cytoplasm YIL050W PCL7 hom FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CELL DIVISION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00987501525012045 0.567409865112601 1708 Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated regulation of glycogen catabolic process|regulation of cyclin-dependent protein kinase activity|regulation of protein stability|regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YFL032W_d YFL032W hom 0.00985929143831918 0.568026995479693 1709 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Unknown Unknown Unknown YDR481C PHO8 hom FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.00984371282353328 0.568638743508973 1710 Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN nicotinamide nucleotide metabolic process|protein dephosphorylation nucleotide phosphatase activity|alkaline phosphatase activity fungal-type vacuole membrane YMR207C HFA1 hom FT KETONE METABOLISM|LIPID METABOLISM|MITOCHONDRION 0.0098134715851395 0.569827167729007 1711 Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress fatty acid biosynthetic process acetyl-CoA carboxylase activity mitochondrion YPL235W RVB2 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00978638586239955 0.571352296463627 1712 ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly regulation of transcription from RNA polymerase II promoter|rRNA processing|chromatin remodeling|box C/D snoRNP assembly|histone exchange ATP-dependent 5'-3' DNA helicase activity|ATP-dependent 3'-5' DNA helicase activity nuclear telomeric heterochromatin|ASTRA complex|R2TP complex|Swr1 complex|nucleus|Ino80 complex YLR451W LEU3 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.00978476044117135 0.571013973940301 1713 Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YPL139C UME1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00975878124445878 0.571979398382359 1714 Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of transcription from RNA polymerase I promoter|negative regulation of chromatin silencing at telomere|positive regulation of transcription from RNA polymerase II promoter|regulation of meiosis transcription corepressor activity Rpd3S complex|Rpd3L-Expanded complex|nucleus|Rpd3L complex YDL193W NUS1 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM 0.00973811399270174 0.57330920361118 1715 Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY protein glycosylation prenyltransferase activity nuclear envelope|lipid particle|endoplasmic reticulum YBR099C_d YBR099C hom 0.00973719998555366 0.572829747658285 1716 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Unknown Unknown Unknown YDL194W SNF3 hom FT CELL CYCLE|SIGNALING|PLASMA MEMBRANE 0.00973246166971527 0.573016528029221 1717 Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication mannose transport|glucose transport|signal transduction|negative regulation of meiosis|detection of glucose|fructose transport glucose transmembrane transporter activity|receptor activity|glucose binding integral to membrane|plasma membrane YJL168C SET2 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.00972285942288211 0.573395128959339 1718 Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p negative regulation of histone H3-K14 acetylation|DNA-dependent transcription, elongation|ascospore formation|regulation of transcription, DNA-dependent|histone deacetylation|regulation of DNA-dependent DNA replication initiation|histone methylation|negative regulation of antisense RNA transcription|Unknown|negative regulation of reciprocal meiotic recombination|positive regulation of histone acetylation histone methyltransferase activity|histone methyltransferase activity (H3-K36 specific) nucleus YER013W PRP22 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEUS 0.0096982260898452 0.574423993331691 1719 DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes spliceosomal complex disassembly|generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity|ATP-dependent RNA helicase activity U2-type post-spliceosomal complex|U2-type catalytic step 2 spliceosome YLR171W_d YLR171W hom 0.00969271392907144 0.574926943556821 1720 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR103C OST2 het FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00967323700762406 0.575353546793482 1721 Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YBR146W MRPS9 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00967057019399816 0.575458885715652 1722 Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YOR067C ALG8 hom FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00966838224579456 0.575545316293732 1723 Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p oligosaccharide-lipid intermediate biosynthetic process|protein N-linked glycosylation dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YHR190W ERG9 het FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00966565800231402 0.575652940725913 1724 Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway ergosterol biosynthetic process squalene synthase activity|farnesyl-diphosphate farnesyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YLR125W_p YLR125W hom 0.00964627815964833 0.576418838210935 1725 Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene biological_process molecular_function cellular_component YGR078C PAC10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.00964537987126353 0.577194358167938 1726 Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding tubulin complex assembly tubulin binding prefoldin complex|polysome|cytoplasm YDL159W-A_p YDL159W-A hom 0.00964443965801328 0.576491521319731 1727 Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function cellular_component YPR120C CLB5 hom FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00961125213409762 0.577804295372936 1728 B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase spindle assembly|G1/S transition of mitotic cell cycle|regulation of S phase of mitotic cell cycle|G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation|premeiotic DNA replication|positive regulation of DNA replication cyclin-dependent protein kinase regulator activity nucleus YPR134W MSS18 hom FT RNA PROCESSING|MITOCHONDRION 0.00958337090959077 0.57896486216914 1729 Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Group I intron splicing molecular_function mitochondrion YMR029C FAR8 hom FT CELL CYCLE|SIGNALING|ENDOPLASMIC RETICULUM 0.00957277368569705 0.579328116558914 1730 Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YMR112C MED11 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0095462137735892 0.580381039236251 1731 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter TFIIH-class transcription factor binding|TFIIH-class transcription factor recruiting transcription factor activity core mediator complex YPR154W PIN3 hom FT CYTOSKELETON ORGANIZATION|NUCLEUS 0.00950218834493669 0.582128323910473 1732 Protein that induces appearance of [PIN+] prion when overproduced actin cytoskeleton organization molecular_function nucleus|cytoplasm YKR093W PTR2 hom FF|FT PROTEIN LOCALIZATION|PLASMA MEMBRANE 0.00948952969693507 0.582631175565246 1733 Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p dipeptide transport|tripeptide transport|peptide transport dipeptide transporter activity|tripeptide transporter activity|peptide transporter activity integral to membrane|plasma membrane YNL265C IST1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT 0.00948743825727971 0.582714275335772 1734 Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p late endosome to vacuole transport via multivesicular body sorting pathway molecular_function endosome|cytoplasm YGR260W TNA1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.00945523928905275 0.583994343304598 1735 High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) nicotinamide mononucleotide transport nicotinamide mononucleotide transmembrane transporter activity mitochondrion|integral to membrane|integral to plasma membrane YBL008W-A_p YBL008W-A hom 0.00944985167292134 0.584545142297561 1736 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YFR009W GCN20 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|NUCLEOTIDE METABOLISM|RIBOSOME 0.00944815091155414 0.584556660171355 1737 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA regulation of translational elongation ATPase activity cytosol|cytosolic ribosome YPL087W YDC1 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00944797754276776 0.584283214321286 1738 Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication ceramide catabolic process|ceramide biosynthetic process dihydroceramidase activity integral to membrane|endoplasmic reticulum YMR261C TPS3 hom FT CARBOHYDRATE METABOLISM 0.00941695516123941 0.585518027146148 1739 Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication trehalose biosynthetic process|response to stress enzyme regulator activity|alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity|trehalose-phosphatase activity alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) YGL034C_d YGL034C hom 0.00941390126325225 0.585639649759032 1740 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR182W SWI6 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.00938549214146267 0.586994821418451 1741 Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of reciprocal meiotic recombination|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity SBF transcription complex|MBF transcription complex|nucleus|cytoplasm YGR238C KEL2 hom FT CELL CYCLE|SITE OF POLARIZED GROWTH 0.00937602997972214 0.587148860748449 1742 Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate conjugation with cellular fusion|negative regulation of exit from mitosis molecular_function mating projection tip|cellular bud neck|cellular bud tip YKR030W GMH1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.0093757896263082 0.587158444833775 1743 Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting transport molecular_function integral to membrane|integral to Golgi membrane YNL152W INN1 het FT CELL CYCLE|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.0093575735495256 0.587885020946383 1744 Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis cytokinesis after mitosis|chromosome organization|barrier septum assembly involved in cell cycle cytokinesis|cytokinesis phospholipid binding cellular bud neck contractile ring|cytoplasm YLR427W MAG2 hom 0.00934037795604787 0.588571274586075 1745 Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 biological_process molecular_function cytoplasm YER180C-A SLO1 hom FT PROTEIN LOCALIZATION 0.00930578469627011 0.589952965501011 1746 Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO intracellular protein transport small GTPase regulator activity cytoplasm YJL031C BET4 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION 0.00930386483858803 0.590584589995057 1747 Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YAL051W OAF1 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GENE SILENCING|NUCLEUS 0.00929613891797749 0.590338494113603 1748 Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication cellular response to oleic acid|positive regulation of fatty acid beta-oxidation|positive regulation of chromatin silencing at telomere|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YCR006C_d YCR006C hom 0.00928275120691627 0.590873774733278 1749 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR208W SEC13 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|NUCLEAR PORE 0.00924121360003459 0.592535990734445 1750 Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress positive regulation of transcription, DNA-dependent|ER-associated protein catabolic process|positive regulation of GTPase activity|nuclear pore distribution|COPII-coated vesicle budding structural molecule activity COPII vesicle coat|nuclear pore outer ring|Seh1-associated complex YER004W FMP52 hom FT ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00922971699041119 0.592996430574284 1751 Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function mitochondrial outer membrane|mitochondrion|endoplasmic reticulum YIR015W RPR2 het FT RNA PROCESSING|NUCLEUS 0.00921534919453699 0.593572091850912 1752 Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress tRNA processing|intronic box C/D snoRNA processing ribonuclease P activity nucleolar ribonuclease P complex YNL232W CSL4 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00921143686371602 0.593838996081685 1753 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nucleus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex)|cytoplasm YDL164C CDC9 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.0091631877440813 0.595664142776027 1754 DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination lagging strand elongation|base-excision repair|mitotic cell cycle|DNA ligation|DNA recombination|nucleotide-excision repair DNA ligase (ATP) activity mitochondrion|nucleus|replication fork YHR028C DAP2 hom FT PROTEOLYSIS 0.00914567782796399 0.596367169650914 1755 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p protein processing dipeptidyl-peptidase activity fungal-type vacuole membrane YJL082W IML2 hom FT NUCLEUS|MITOCHONDRION 0.00914416161241021 0.596482823812994 1756 Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication biological_process molecular_function mitochondrial outer membrane|mitochondrion|nucleus|cytoplasm YIL087C AIM19 hom FT MITOCHONDRION 0.00913122985554305 0.596947543114655 1757 Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth biological_process molecular_function mitochondrion YLR439W MRPL4 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.00912563152132138 0.597172496613681 1758 Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOR132W VPS17 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.00912336847716147 0.597263441689064 1759 Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes retrograde transport, endosome to Golgi protein transporter activity|phosphatidylinositol-3-phosphate binding endosome|retromer complex, outer shell|retromer complex YJR037W_d YJR037W hom 0.00910998080065212 0.598238772807468 1760 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Unknown Unknown Unknown YOR192C THI72 hom 0.00910877885871516 0.598068397858288 1761 Transporter of thiamine or related compound; shares sequence similarity with Thi7p thiamine transport transporter activity integral to membrane|membrane YLR075W RPL10 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00908965192669091 0.598619155399636 1762 Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects cytoplasmic translation|translational termination|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YDR001C NTH1 hom FT CARBOHYDRATE METABOLISM 0.00908211687034695 0.59892232423125 1763 Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication trehalose catabolic process|response to stress alpha,alpha-trehalase activity cytosol|cytoplasm YOL037C_d YOL037C hom 0.00906709584806185 0.599581320678216 1764 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Unknown Unknown Unknown YBL034C STU1 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|NUCLEUS|CHROMOSOME|CYTOSKELETON 0.00906345678254393 0.599673401891487 1765 Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles microtubule nucleation structural constituent of cytoskeleton spindle pole body YCL050C APA1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.00906036864983804 0.599797741839053 1766 AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication nucleotide biosynthetic process|nucleoside catabolic process bis(5'-nucleosyl)-tetraphosphatase activity|sulfate adenylyltransferase (ADP) activity nucleus|cytoplasm YMR265C_p YMR265C hom 0.00902460599563054 0.601238530948758 1767 Putative protein of unknown function biological_process molecular_function cellular_component YKL006W RPL14A hom FT TRANSLATION|RIBOSOME 0.0090209497175444 0.601440146559766 1768 Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YJL089W SIP4 hom FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00900991353998337 0.601830906000026 1769 C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YJL066C MPM1 hom FT MITOCHONDRION 0.008974524465308 0.603258812631856 1770 Mitochondrial intermembrane space protein of unknown function biological_process molecular_function mitochondrion|extrinsic to membrane YER146W LSM5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00894088044515796 0.604617718893991 1771 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YPL077C_p YPL077C hom 0.00892366687206127 0.605313517947507 1772 Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication biological_process molecular_function cellular_component YOR118W RTC5 hom 0.00891769457342845 0.605555011195275 1773 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity biological_process molecular_function cytoplasm YNL054W VAC7 hom FT LIPID METABOLISM 0.00891603493950033 0.605622127081846 1774 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock positive regulation of phosphatidylinositol biosynthetic process|positive regulation of kinase activity molecular_function fungal-type vacuole membrane|integral to membrane|PAS complex|vacuolar membrane|cytoplasm YHR155W YSP1 hom FF|FT MITOCHONDRION 0.00890946966491094 0.60588766056643 1775 Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication biological_process molecular_function mitochondrion YPL023C MET12 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 0.00887966424242745 0.607093799602069 1776 Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR biological_process methylenetetrahydrofolate reductase (NADPH) activity cellular_component YCR010C ADY2 hom FT CELL CYCLE|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION|PLASMA MEMBRANE 0.00884409754078048 0.608587926158432 1777 Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication nitrogen utilization|ammonium transport|acetate transport|transmembrane transport acetate transmembrane transporter activity|ammonium transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YKL069W YKL069W hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.00884156658073895 0.608690493150987 1778 Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress cellular response to oxidative stress methionine-R-sulfoxide reductase activity nucleus|cytoplasm YDR140W MTQ2 hom FT TRANSLATION|NUCLEUS 0.00882642894319733 0.609250739825389 1779 S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC peptidyl-glutamine methylation|regulation of translation protein methyltransferase activity|S-adenosylmethionine-dependent methyltransferase activity eRF1 methyltransferase complex|nucleus|cytoplasm YER037W PHM8 hom FT RESPONSE TO NUTRIENT LEVELS|NUCLEUS 0.00881941007598374 0.609535378038397 1780 Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p cellular response to phosphate starvation lysophosphatidic acid phosphatase activity nucleus|cytoplasm YGL077C HNM1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00881280280242434 0.609803378749575 1781 Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol choline transport|ethanolamine transport|glycine betaine transport choline transmembrane transporter activity|ethanolamine transmembrane transporter activity integral to membrane|plasma membrane YBL060W YEL1 hom FT PROTEIN LOCALIZATION|LIPID METABOLISM|SIGNALING|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00879705556235907 0.610442320794828 1782 Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip positive regulation of phosphatidylinositol biosynthetic process|protein localization|actin cortical patch localization ARF guanyl-nucleotide exchange factor activity cellular bud neck|cellular bud tip|cytoplasm YBR266C_d SLM6 hom 0.00879452968525617 0.610544835532627 1783 Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Unknown Unknown Unknown YER096W SHC1 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM 0.00878635253139921 0.610876764201122 1784 Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication ascospore formation|chitin biosynthetic process enzyme activator activity prospore membrane YPL011C TAF3 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00878131705359897 0.611081205106708 1785 TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly transcription factor TFIID complex YPL218W SAR1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00877787397103842 0.611221012052053 1786 GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport regulation of COPII vesicle coating GTPase activity COPII vesicle coat YMR302C YME2 hom FT RNA PROCESSING|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00876839478322537 0.611871616829942 1787 Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases mitochondrial genome maintenance molecular_function mitochondrion|integral to mitochondrial inner membrane YCR063W BUD31 hom FT CELL CYCLE|RNA PROCESSING|CELL DIVISION|NUCLEUS 0.00876460732350115 0.611759838909177 1788 Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis RNA splicing|mRNA splicing, via spliceosome|cellular bud site selection molecular_function U2 snRNP|nucleus YLR092W SUL2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00875947050466371 0.61196852799752 1789 High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates sulfate transport sulfate transmembrane transporter activity integral to membrane|plasma membrane YLR241W_p YLR241W hom 0.00875217866514003 0.61231785392696 1790 Putative protein of unknown function, may be involved in detoxification biological_process molecular_function integral to membrane YLR127C APC2 het FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.00870124165102427 0.614494776326442 1791 Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p mitotic metaphase/anaphase transition|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|exit from mitosis|cyclin catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YOR183W_p FYV12 hom 0.00869695440005323 0.614510834426525 1792 Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cellular_component YCL016C DCC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|CHROMOSOME 0.00868464190623771 0.615012086970693 1793 Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance maintenance of DNA trinucleotide repeats|response to DNA damage stimulus|mitotic sister chromatid cohesion molecular_function Ctf18 RFC-like complex YML078W CPR3 hom FT MITOCHONDRION 0.00867585587535186 0.615369883965435 1794 Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria protein folding|apoptotic process peptidyl-prolyl cis-trans isomerase activity mitochondrion YCL012C YCL012C hom 0.00865678371589639 0.616620642840708 1795 Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene biological_process molecular_function cellular_component YCR107W AAD3 hom FT OXIDATION-REDUCTION PROCESS 0.00865336805445728 0.616286080715791 1796 Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YGL253W HXK2 hom FT CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS|MITOCHONDRION 0.00861907435506114 0.617684422154995 1797 Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication replicative cell aging|fructose import|regulation of transcription by glucose|glucose import|mannose metabolic process|glycolysis|glucose metabolic process|regulation of cell size|fructose metabolic process hexokinase activity cytosol|mitochondrion|nucleus YCR077C PAT1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|TRANSLATION|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|RIBOSOME 0.00860770916210535 0.618252912895399 1798 Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|chromosome segregation|regulation of translational initiation|formation of translation preinitiation complex|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly molecular_function cytosolic small ribosomal subunit|cytoplasmic mRNA processing body YML080W DUS1 hom FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00860366533701228 0.618313183607169 1799 Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 tRNA modification tRNA dihydrouridine synthase activity nucleus YAR002C-A ERP1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00860106072576008 0.618681322095095 1800 Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|ER to Golgi transport vesicle YGL215W CLG1 hom FT CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS 0.00859993828927528 0.618517648353382 1801 Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 positive regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YPR061C JID1 hom FT MITOCHONDRION 0.00858673562698586 0.619004318765559 1802 Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae biological_process molecular_function mitochondrion YJL130C URA2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|MITOCHONDRION 0.00855286923364256 0.62038788426129 1803 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP glutamine metabolic process|'de novo' pyrimidine nucleobase biosynthetic process|negative regulation of pyrimidine nucleobase metabolic process aspartate carbamoyltransferase activity|carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity mitochondrion|integral to membrane|cytoplasm YMR076C PDS5 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.0085486535877387 0.620716550102329 1804 Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis double-strand break repair|meiosis|synapsis|mitotic chromosome condensation|mitotic sister chromatid cohesion structural molecule activity condensed nuclear chromosome YHL019C APM2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00853518760789431 0.621110777380827 1805 Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YJL179W PFD1 hom FT CYTOSKELETON ORGANIZATION 0.00849931424840962 0.622578542163492 1806 Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin protein folding|cytoskeleton organization unfolded protein binding prefoldin complex YDL204W RTN2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00848801036420264 0.623041353788376 1807 Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum tubular network maintenance molecular_function endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YHL044W_p YHL044W hom FT PLASMA MEMBRANE 0.00848175607990683 0.623297485106257 1808 Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern biological_process molecular_function plasma membrane YNL292W PUS4 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.0084742701319305 0.623604116558632 1809 Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) tRNA modification pseudouridine synthase activity mitochondrion|nucleus YKR076W ECM4 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS 0.00847072731959857 0.62374925621345 1810 Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization|glutathione metabolic process glutathione transferase activity cytoplasm YBR299W MAL32 hom FT CARBOHYDRATE METABOLISM 0.00843820435350813 0.625082317852336 1811 Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YLR431C ATG23 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.00841445253447266 0.626056640362125 1812 Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p protein localization to pre-autophagosomal structure|positive regulation of macroautophagy|CVT pathway molecular_function pre-autophagosomal structure|extrinsic to membrane YIL162W SUC2 hom FT CARBOHYDRATE METABOLISM|MITOCHONDRION 0.0084138434911693 0.626081632498084 1813 Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively sucrose catabolic process beta-fructofuranosidase activity mitochondrion|extracellular region|cytoplasm YLR068W FYV7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00841189151239248 0.626419191099185 1814 Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YGL190C CDC55 hom FT CELL CYCLE|SIGNALING|NUCLEUS|SITE OF POLARIZED GROWTH 0.00838094321476019 0.627432335098376 1815 Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion negative regulation of exit from mitosis|positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|protein dephosphorylation protein phosphatase type 2A regulator activity nucleus|cellular bud neck|cytoplasm|cellular bud tip YCR099C_p YCR099C hom 0.00835199398348575 0.628621862930429 1816 Putative protein of unknown function biological_process molecular_function integral to membrane YCR106W RDS1 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00833765882713536 0.629211253665518 1817 Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide response to xenobiotic stimulus sequence-specific DNA binding|DNA binding nucleus YDL089W NUR1 hom FT ENDOMEMBRANE SYSTEM|NUCLEUS 0.00833159155652255 0.629665220963865 1818 Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate maintenance of rDNA molecular_function nuclear periphery YIL013C PDR11 hom FT NUCLEOTIDE METABOLISM 0.00832200930871404 0.630110301932164 1819 ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth sterol transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YLR260W LCB5 hom FT LIPID METABOLISM|SIGNALING|PROTEIN PHOSPHORYLATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00832004602046281 0.630600001311279 1820 Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules response to heat|calcium-mediated signaling|sphingolipid metabolic process D-erythro-sphingosine kinase activity Golgi apparatus|membrane YDR245W MNN10 hom FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00831454221118167 0.630315266936497 1821 Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family N-glycan processing|cell wall mannoprotein biosynthetic process|substituted mannan metabolic process|barrier septum assembly alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YNL250W RAD50 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS|MITOCHONDRION 0.00828408708147368 0.631568590562888 1822 Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress base-excision repair|meiotic DNA double-strand break processing|telomere maintenance via recombination|meiosis|meiotic DNA double-strand break formation|double-strand break repair via nonhomologous end joining|telomere maintenance|double-strand break repair via break-induced replication|negative regulation of endodeoxyribonuclease activity double-stranded DNA binding|single-stranded telomeric DNA binding|ATPase activity|double-stranded telomeric DNA binding|G-quadruplex DNA binding|telomeric DNA binding|adenylate kinase activity mitochondrion|nucleus|Mre11 complex YPR164W MMS1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.00825804274344293 0.6324889633882 1823 Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay replication fork processing|negative regulation of transposition, RNA-mediated|recombinational repair|nonfunctional rRNA decay molecular_function Cul8-RING ubiquitin ligase complex YLR266C PDR8 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00823897557461499 0.633275013921868 1824 Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding nucleus YJL065C DLS1 hom FT GENE SILENCING|NUCLEUS 0.00822121377242373 0.63420997286545 1825 Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication chromatin silencing at telomere molecular_function chromatin accessibility complex|nucleus YGR232W NAS6 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.00821130149821783 0.634972923388171 1826 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 proteolysis|proteasome regulatory particle assembly molecular_function cytosol|nucleus|proteasome regulatory particle YPR107C YTH1 het FF|FT RNA PROCESSING|NUCLEUS 0.00820347473512033 0.634992188874984 1827 Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YJL153C INO1 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM 0.00818651998932066 0.635792699073435 1828 Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element inositol biosynthetic process inositol-3-phosphate synthase activity cytoplasm YIL012W_d YIL012W hom 0.00817274125743861 0.636159813781884 1829 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCL061C MRC1 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00815263313068179 0.636839682424584 1830 S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres maintenance of DNA repeat elements|intra-S DNA damage checkpoint|DNA repair|DNA replication checkpoint|telomere maintenance|replication fork protection|DNA replication|mitotic sister chromatid cohesion|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere molecular_function nuclear chromosome|replication fork protection complex|nucleus|nuclear replication fork YGR289C MAL11 hom FT CARBOHYDRATE METABOLISM|PLASMA MEMBRANE 0.00813953232082813 0.637582078795806 1831 Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization trehalose transport|maltose transport|disaccharide catabolic process trehalose transmembrane transporter activity|alpha-glucoside:hydrogen symporter activity|maltose:hydrogen symporter activity integral to membrane|plasma membrane YJL208C NUC1 hom FT NUCLEUS|MITOCHONDRION 0.00811456928887905 0.638413835989163 1832 Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy DNA catabolic process|RNA catabolic process|apoptotic process|DNA recombination ribonuclease activity|endodeoxyribonuclease activity|exodeoxyribonuclease activity mitochondrion|mitochondrial inner membrane|nucleus YCR093W CDC39 het FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|NUCLEUS 0.0081068621384889 0.639233444159893 1833 Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex|nucleus|cytoplasm YLL027W ISA1 hom FT KETONE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.0081061602628063 0.638861871969634 1834 Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins biotin biosynthetic process|iron-sulfur cluster assembly iron-sulfur cluster binding|iron ion binding mitochondrial matrix YOR066W MSA1 hom FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|NUCLEUS 0.00810212181430162 0.638928963070512 1835 Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation G1/S transition of mitotic cell cycle|regulation of transcription involved in G1 phase of mitotic cell cycle|regulation of cell size molecular_function nucleus|cytoplasm YLL014W EMC6 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00810065548058886 0.639239731699262 1836 Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane|endoplasmic reticulum YKL186C MTR2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00807747032391716 0.639949655777271 1837 mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA poly(A)+ mRNA export from nucleus|ribosomal large subunit export from nucleus protein binding integral to membrane|nuclear RNA export factor complex|nuclear pore YER130C_p YER130C hom FT NUCLEUS 0.00807328557178335 0.640322506056099 1838 Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress biological_process sequence-specific DNA binding cellular_component YER161C SPT2 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION|NUCLEUS 0.00807149996831872 0.640196960386417 1839 Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins negative regulation of transcription from RNA polymerase II promoter|chromatin organization|RNA polyadenylation|transcription elongation from RNA polymerase II promoter DNA binding|DNA secondary structure binding nucleus YPL128C TBF1 het FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00802473030880661 0.6421356380086 1840 Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation telomere maintenance via telomere lengthening|negative regulation of chromatin silencing|snoRNA transcription from an RNA polymerase II promoter|negative regulation of telomere maintenance via telomerase RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|chromatin insulator sequence binding|telomeric DNA binding nuclear chromosome, telomeric region|nucleus YNR012W URK1 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.0080111686978616 0.642698245549636 1841 Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP pyrimidine-containing compound salvage uridine kinase activity nucleus|cytoplasm YKL071W_p YKL071W hom FT OXIDATION-REDUCTION PROCESS 0.00800576608312139 0.642922431758512 1842 Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function integral to membrane|cytoplasm YGR180C RNR4 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00798906095999182 0.643863203286583 1843 Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress cofactor biosynthetic process|deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ribonucleoside-diphosphate reductase complex|nucleus|cytoplasm YDL022W GPD1 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00794913113760951 0.645471602390647 1844 NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity NADH oxidation|intracellular accumulation of glycerol glycerol-3-phosphate dehydrogenase [NAD+] activity cytosol|peroxisome|nucleus YMR269W TMA23 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.00791867363743314 0.646540882519609 1845 Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan ribosomal small subunit biogenesis molecular_function ribosome|nucleolus YER125W RSP5 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGAN 0.00790183297486617 0.647241534126586 1846 E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p cellular response to UV|late endosome to vacuole transport via multivesicular body sorting pathway|chromatin assembly or disassembly|response to drug|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent endocytosis|protein monoubiquitination|positive regulation of fatty acid biosynthetic process|positive regulation of receptor-mediated endocytosis|regulation of phosphate metabolic process|regulation of ubiquinone biosynthetic process|regulation of dolichol biosynthetic process|regulation of multivesicular body size|positive regulation of transcription from RNA polymerase II promoter|regulation of nitrogen utilization|proteasomal ubiquitin-dependent protein catabolic process|positive regulation of endocytosis|regulation of actin cytoskeleton organization|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|regulation of protein localization|mitochondrion organization|regulation of mRNA export from nucleus|regulation of ergosterol biosynthetic process|ribophagy|Unknown|protein polyubiquitination ubiquitin binding|ubiquitin-protein ligase activity|phosphatidylinositol binding mitochondrion|ubiquitin ligase complex|Golgi apparatus|cytoplasm|cellular bud tip|endosome membrane|extrinsic to internal side of plasma membrane|plasma membrane|nucleus YDR392W SPT3 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.00789998934261746 0.647318256985873 1847 Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters conjugation with cellular fusion|histone acetylation|pseudohyphal growth|ascospore formation|chromatin modification|invasive growth in response to glucose limitation|transcription from RNA polymerase II promoter transcription cofactor activity SLIK (SAGA-like) complex|SAGA complex YIL014C-A_p YIL014C-A hom 0.00786937903987387 0.648592653346877 1848 Putative protein of unknown function biological_process molecular_function cellular_component YPR044C_d OPI11 hom 0.00786105152466453 0.649086046478399 1849 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Unknown Unknown Unknown YNL103W MET4 het FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.00785931572380818 0.649011843617133 1850 Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p response to cadmium ion|sulfur amino acid metabolic process|response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity nucleus YER097W_d YER097W hom 0.00783544538057503 0.650006613824918 1851 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR135C_d IRC14 hom 0.007812396573908 0.650967739475988 1852 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YNL108C_p YNL108C hom FT NUCLEUS 0.00780657937579401 0.651210405988009 1853 Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YJR066W TOR1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS|PLASMA MEMBRANE 0.00779219395570159 0.651810657558865 1854 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication regulation of cell cycle|G1 phase of mitotic cell cycle|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell growth|response to DNA damage stimulus|mitochondria-nucleus signaling pathway|ribosome biogenesis|meiosis|signal transduction|TOR signaling cascade|fungal-type cell wall organization|negative regulation of autophagy protein binding|protein kinase activity TORC1 complex|fungal-type vacuole membrane|endosome membrane|extrinsic to internal side of plasma membrane|plasma membrane|nucleus|Golgi membrane|cytoplasm YMR015C ERG5 hom FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|ENDOPLASMIC RETICULUM 0.00779197254158163 0.651819898133276 1855 C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs ergosterol biosynthetic process C-22 sterol desaturase activity endoplasmic reticulum YMR242W-A_p YMR242W-A hom 0.00774730291215535 0.653685247418223 1856 Putative protein of unknown function biological_process molecular_function cellular_component YPR159W KRE6 hom FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00773573236736606 0.65426519969052 1857 Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p fungal-type cell wall organization|(1->6)-beta-D-glucan biosynthetic process glucosidase activity integral to endoplasmic reticulum membrane|integral to membrane|site of polarized growth|transport vesicle|plasma membrane YKR052C MRS4 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00770014667070517 0.655656783775712 1858 Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication mitochondrial iron ion transport|RNA splicing iron ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YCR046C IMG1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0076965894669728 0.656045757998536 1859 Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YOR247W SRL1 hom FT CELL WALL ORG/BIOGENESIS|SITE OF POLARIZED GROWTH 0.00769515027386455 0.655865817019589 1860 Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication fungal-type cell wall organization|nucleobase-containing compound metabolic process molecular_function fungal-type cell wall|fungal-type vacuole|cytoplasm|cellular bud tip YCL036W GFD2 hom 0.00767941957667807 0.656524114900087 1861 Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component YER006W NUG1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00766951308555016 0.656938818387168 1862 GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus ribosomal large subunit export from nucleus|rRNA processing RNA binding|GTPase activity nucleolus|nucleus|preribosome, large subunit precursor YDL196W_d YDL196W het FF 0.00765001204973241 0.657898842264087 1863 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Unknown Unknown Unknown YPL135W ISU1 hom FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM|MITOCHONDRION 0.00764263195158202 0.658064642772433 1864 Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable cellular iron ion homeostasis|iron-sulfur cluster assembly|tRNA wobble uridine modification ferrous iron binding mitochondrion|mitochondrial matrix YFL008W SMC1 het FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME 0.00762219796762685 0.659540961219496 1865 Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure double-strand break repair|mitotic sister chromatid cohesion|mitotic sister chromatid segregation double-stranded DNA binding|ATPase activity|AT DNA binding|DNA secondary structure binding nuclear mitotic cohesin complex YPR170C_d YPR170C hom 0.00757908484818536 0.660729175950092 1866 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Unknown Unknown Unknown YBR134W_d YBR134W hom 0.00752597203542261 0.662959503060339 1867 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL296W_d YNL296W hom 0.00751874465071466 0.663263228826043 1868 Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Unknown Unknown Unknown YGL025C PGD1 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00751506162921734 0.663653604617528 1869 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II activating transcription factor binding|RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex YLR434C_d YLR434C hom 0.0075068956114903 0.663761296075832 1870 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Unknown Unknown Unknown YMR011W HXT2 hom FT PLASMA MEMBRANE 0.00748676717683737 0.664607723112979 1871 High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose glucose transport pentose transmembrane transporter activity|mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YDL045W-A MRP10 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION|RIBOSOME 0.0074803522521938 0.665112263637519 1872 Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs mitochondrial translation|mitochondrial respiratory chain complex assembly structural constituent of ribosome mitochondrial small ribosomal subunit YDR376W ARH1 het FT KETONE METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.00747694246287132 0.665021020585519 1873 Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability ubiquinone biosynthetic process|cellular iron ion homeostasis NADPH-adrenodoxin reductase activity mitochondrion|mitochondrial inner membrane YHR171W ATG7 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|MITOCHONDRION 0.00747368649649645 0.665158012191846 1874 Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation C-terminal protein lipidation|protein modification by small protein conjugation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway APG8 activating enzyme activity|APG12 activating enzyme activity cytosol|mitochondrion|membrane|pre-autophagosomal structure YFR035C_p YFR035C hom 0.0074604278178541 0.665715973516615 1875 Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YKL176C LST4 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM 0.00742122765379722 0.667366704503815 1876 Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface intracellular protein transport|vesicle-mediated transport|protein transport|Golgi to plasma membrane transport protein transporter activity vesicle coat YML112W CTK3 hom FF|FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00742050439540911 0.667676999039236 1877 Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress mRNA 3'-end processing|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation cyclin-dependent protein kinase activity nucleoplasm|nucleolus|carboxy-terminal domain protein kinase complex YPL244C HUT1 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00735967676115392 0.670100815569279 1878 Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 UDP-galactose transport|UDP-glucose transport UDP-galactose transmembrane transporter activity integral to membrane YNL097C PHO23 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00735436159733419 0.670186167664582 1879 Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of transcription from RNA polymerase I promoter|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA|chromatin modification|positive regulation of transcription from RNA polymerase II promoter methylated histone residue binding histone deacetylase complex|Rpd3L-Expanded complex|nucleus|Rpd3L complex YIL037C PRM2 hom 0.00730057665207798 0.672457432088545 1880 Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion karyogamy involved in conjugation with cellular fusion molecular_function integral to membrane YFL012W_p YFL012W hom 0.00729999680137841 0.672481934720073 1881 Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin biological_process molecular_function cellular_component YFL019C_d YFL019C hom 0.00727174735311118 0.67367608647605 1882 Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Unknown Unknown Unknown YJL101C GSH1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO OXIDATIVE STRESS 0.00727112496469568 0.674115313376509 1883 Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress response to cadmium ion|response to hydrogen peroxide|glutathione biosynthetic process glutamate-cysteine ligase activity intracellular YEL046C GLY1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00725875502008772 0.674362941462366 1884 Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis threonine catabolic process|glycine biosynthetic process threonine aldolase activity|L-allo-threonine aldolase activity cytosol YDL140C RPO21 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|MITOCHONDRION|RNA POL II, HOLOENZYME 0.00724448728607705 0.674829195332491 1885 RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity mitochondrion|DNA-directed RNA polymerase II, core complex YNL271C BNI1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00719124127355665 0.677174586901148 1886 Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 barbed-end actin filament capping|actin nucleation|actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assembly|establishment of mitotic spindle orientation|formin-nucleated actin cable assembly profilin binding incipient cellular bud site|actin filament|polarisome|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YER184C_p YER184C hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00719068400193511 0.677152756495284 1887 Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source biological_process sequence-specific DNA binding cellular_component YOL120C RPL18A het FF|FT TRANSLATION|RIBOSOME 0.00718703564621504 0.677261914297236 1888 Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR006C TSR3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00717163725899108 0.677914513558282 1889 Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress maturation of SSU-rRNA molecular_function nucleus|cytoplasm YPR127W YPR127W hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00717010740262989 0.677979363624432 1890 Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YGL040C HEM2 het FT COFACTOR METABOLISM|NUCLEUS 0.00714671657086342 0.678971189358546 1891 Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus heme biosynthetic process porphobilinogen synthase activity nucleus|cytoplasm YKR007W MEH1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS 0.00712330355087765 0.680189965842429 1892 Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification microautophagy|vacuolar acidification molecular_function fungal-type vacuole membrane|EGO complex|late endosome membrane YDL216C RRI1 hom FT SIGNALING|PROTEOLYSIS|NUCLEUS 0.00710762566709713 0.680900047723809 1893 Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation metalloendopeptidase activity signalosome YPR146C_d YPR146C hom 0.00710088584444361 0.680916129283125 1894 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR032C HMS1 hom FT NUCLEUS 0.00710036445401777 0.680938268006664 1895 Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant pseudohyphal growth sequence-specific DNA binding transcription factor activity cellular_component YDR169C-A_p YDR169C-A hom 0.0070809786204628 0.681761603441131 1896 Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHR157W REC104 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME 0.00702428611226884 0.68417156143405 1897 Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YDL072C YET3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.0070207553637449 0.684321757742187 1898 Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|endoplasmic reticulum YKL073W LHS1 hom FT PROTEIN LOCALIZATION|NUCLEOTIDE METABOLISM|ENDOPLASMIC RETICULUM 0.00701577250625053 0.684533747287966 1899 Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway posttranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|adenyl-nucleotide exchange factor activity|ATP binding endoplasmic reticulum lumen YOR105W_p YOR105W hom 0.00700302839668612 0.685076042751405 1900 Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YNL094W APP1 hom FT CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00699663412545076 0.685348196999263 1901 Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway actin cytoskeleton organization molecular_function actin cytoskeleton|actin cortical patch YOR265W RBL2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00697589836176768 0.686231037358352 1902 Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress tubulin complex assembly|tubulin complex biogenesis tubulin binding|beta-tubulin binding cytoplasm YDL115C IWR1 hom FT PROTEIN LOCALIZATION|NUCLEUS|RNA POL II, HOLOENZYME 0.00697270701889996 0.686366949171351 1903 RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress cellular protein complex localization|nucleocytoplasmic transport protein binding nucleus|DNA-directed RNA polymerase II, holoenzyme|cytoplasm YFR013W IOC3 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.00688060123929834 0.690293871687208 1904 Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YMR316W DIA1 hom 0.00684766200392453 0.691700265574792 1905 Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern pseudohyphal growth|invasive growth in response to glucose limitation molecular_function cytoplasm YDR017C KCS1 hom 0.00681744739251716 0.692991262772934 1906 Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance inositol phosphate biosynthetic process inositol heptakisphosphate kinase activity|inositol hexakisphosphate kinase activity|inositol-1,3,4,5,6-pentakisphosphate kinase activity|inositol heptakisphosphate 5-kinase activity cytoplasm YDR007W TRP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00681111075187992 0.693262125317324 1907 Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) tryptophan biosynthetic process phosphoribosylanthranilate isomerase activity cytoplasm YNL062C GCD10 het FT RNA PROCESSING|TRANSLATION|NUCLEUS 0.00680038344401666 0.69402441475266 1908 Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression tRNA methylation tRNA (adenine-N1-)-methyltransferase activity tRNA (m1A) methyltransferase complex|nucleus YPR004C AIM45 hom FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00679202840161872 0.694078045521796 1909 Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response biological_process molecular_function mitochondrion YIL029C_p YIL029C hom 0.00676952947937707 0.695040506395719 1910 Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W biological_process molecular_function integral to membrane YBR013C_p YBR013C hom 0.006763253299572 0.695309077105813 1911 Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YBR056W YBR056W hom FT CARBOHYDRATE METABOLISM 0.00672971972146305 0.696959645847698 1912 Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function cytoplasm YEL042W GDA1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00672670120030387 0.696873975474169 1913 Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate protein glycosylation guanosine-diphosphatase activity|uridine-diphosphatase activity Golgi apparatus YGR163W GTR2 hom FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|MEMBRANE ORGANIZATION|NUCLEUS|CHROMOSOME 0.00672001089806522 0.697160546043188 1914 Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD microautophagy|positive regulation of transcription from RNA polymerase II promoter GTP binding nuclear chromatin|fungal-type vacuole membrane|integral to membrane|EGO complex|nucleus|late endosome membrane|cytoplasm YPR089W_p YPR089W hom FT GOLGI APPARATUS 0.0066927797378332 0.698327403377375 1915 Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p biological_process molecular_function cellular_component YPR124W CTR1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00665573282523756 0.699916010970868 1916 High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress copper ion import copper uptake transmembrane transporter activity integral to membrane|plasma membrane YKL134C OCT1 hom FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION 0.0066452377089659 0.700366290800365 1917 Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis cellular iron ion homeostasis|protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion YNL221C POP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00663847228276563 0.700784068928101 1918 Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YMR272C SCS7 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00662344552528896 0.701513653112135 1919 Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth inositolphosphoceramide metabolic process fatty acid alpha-hydroxylase activity integral to membrane|endoplasmic reticulum|membrane YNL211C_p YNL211C hom FT MITOCHONDRION 0.00661089646151688 0.701840394156889 1920 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene biological_process molecular_function mitochondrion YDL014W NOP1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00659270020671043 0.702621926388318 1921 Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin box C/D snoRNA 3'-end processing|rRNA processing|snoRNA processing|rRNA methylation rRNA methyltransferase activity small-subunit processome|box C/D snoRNP complex|ribosome|nucleolus|90S preribosome YEL061C CIN8 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.0065865716224604 0.702885220923635 1922 Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation mitotic anaphase B|microtubule depolymerization|spindle pole body separation|mitotic spindle organization in nucleus|mitotic sister chromatid segregation plus-end-directed microtubule motor activity|minus-end-directed microtubule motor activity spindle microtubule|kinesin complex|mitochondrion|condensed nuclear chromosome kinetochore|kinetochore microtubule|astral microtubule YJL149W DAS1 hom FT PROTEOLYSIS|UBIQUITIN LIGASE COMPLEX 0.00658648077299227 0.702889124241338 1923 Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YJL155C FBP26 hom FT CARBOHYDRATE METABOLISM 0.00657268763531128 0.703566079488791 1924 Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress glucose metabolic process 6-phosphofructo-2-kinase activity|fructose-2,6-bisphosphate 2-phosphatase activity cytosol YPR091C NVJ2 hom FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00652531421492119 0.705518897892895 1925 Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments biological_process lipid binding ribosome|nucleus-vacuole junction|endoplasmic reticulum YMR315W YMR315W hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00652400220871747 0.705575344501363 1926 Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress NADPH regeneration oxidoreductase activity nucleus|cytoplasm YDR084C TVP23 hom FT VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00652269306987956 0.705631669361117 1927 Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|cytoplasm YJR070C LIA1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CYTOSKELETON ORGANIZATION|NUCLEUS 0.00650950338462413 0.70632457662397 1928 Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress microtubule cytoskeleton organization|peptidyl-lysine modification to hypusine deoxyhypusine monooxygenase activity nucleus|cytoplasm YGR281W YOR1 hom FT NUCLEOTIDE METABOLISM|PLASMA MEMBRANE 0.00643609094710879 0.709361237875704 1929 Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) transport|response to drug xenobiotic-transporting ATPase activity integral to membrane|plasma membrane YBL106C SRO77 hom FF|FT VESICLE-MEDIATED TRANSPORT|PLASMA MEMBRANE 0.00639334347935755 0.711204757481999 1930 Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication exocytosis|Golgi to plasma membrane transport molecular_function plasma membrane YOL016C CMK2 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.00637800156617657 0.711866802612084 1931 Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication signal transduction|protein phosphorylation protein kinase activity|calmodulin-dependent protein kinase activity cytoplasm YBR292C_d YBR292C hom 0.00636645605764971 0.712365165842361 1932 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Unknown Unknown Unknown YJL062W LAS21 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE 0.00635911288874665 0.712682198495951 1933 Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity integral to endoplasmic reticulum membrane|integral to membrane|integral to plasma membrane YBL069W AST1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|GOLGI APPARATUS|PLASMA MEMBRANE 0.00630514031020652 0.715013920631375 1934 Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication protein targeting to membrane|protein transport into membrane raft molecular_function late endosome|membrane raft|Golgi apparatus|plasma membrane YLR428C_d YLR428C hom 0.00625921443522887 0.717000105848713 1935 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Unknown Unknown Unknown YMR159C ATG16 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.00624885953578512 0.717448195674988 1936 Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C-terminal protein lipidation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway protein binding, bridging|Atg8 ligase activity Atg12-Atg5-Atg16 complex|pre-autophagosomal structure YBL071W-A KTI11 hom FT RNA PROCESSING|NUCLEUS 0.00624446922038898 0.71771881426209 1937 Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs peptidyl-diphthamide biosynthetic process from peptidyl-histidine|tRNA wobble uridine modification electron carrier activity|iron ion binding|zinc ion binding nucleus|cytoplasm YCR073C SSK22 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.00621516032241836 0.718907141291097 1938 MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p MAPK cascade involved in osmosensory signaling pathway|protein phosphorylation protein kinase activity|MAP kinase kinase kinase activity cellular_component YGL092W NUP145 het FT PROTEIN LOCALIZATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00620639914413846 0.719286608453185 1939 Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 double-strand break repair|poly(A)+ mRNA export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|tRNA export from nucleus|protein targeting to nuclear inner membrane|telomere tethering at nuclear periphery|nuclear pore distribution|positive regulation of transcription, DNA-dependent|NLS-bearing substrate import into nucleus|protein import into nucleus nucleocytoplasmic transporter activity|RNA binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore|nuclear pore outer ring YJL188C_d BUD19 hom 0.00619055970223364 0.719972827318796 1940 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Unknown Unknown Unknown YIL144W TID3 het FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.00613587018215557 0.722343895596977 1941 Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering microtubule nucleation|chromosome segregation|protein localization to kinetochore structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YGL234W ADE5,7 hom FT NUCLEOTIDE METABOLISM 0.00612259477228556 0.722919855303285 1942 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities purine nucleobase metabolic process|purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylamine-glycine ligase activity|phosphoribosylformylglycinamidine cyclo-ligase activity cytoplasm YGR101W PCP1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00609807570915026 0.723984038439311 1943 Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases signal peptide processing|regulation of mitochondrion organization serine-type endopeptidase activity integral to membrane|mitochondrial inner membrane YNR052C POP2 hom FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS|SITE OF POLARIZED GROWTH 0.00609416288946857 0.724153913129036 1944 RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter 3'-5'-exoribonuclease activity CCR4-NOT core complex|mating projection tip|cytoplasmic mRNA processing body|cytoplasm YDL019C OSH2 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE 0.00608269704146281 0.724651779984034 1945 Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability endocytosis|maintenance of cell polarity|sterol transport|exocytosis lipid binding|oxysterol binding|sterol transporter activity|1-phosphatidylinositol binding endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum|cellular bud neck YLR172C DPH5 hom 0.00607267800322688 0.725204929728386 1946 Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-diphthamide biosynthetic process from peptidyl-histidine diphthine synthase activity cytoplasm YLR163C MAS1 het FF|FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00605137202853031 0.726326416799494 1947 Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion|mitochondrial processing peptidase complex YPR042C PUF2 hom 0.00602384613773254 0.727326193008502 1948 Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay mRNA binding cytoplasm YPL176C TRE1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|PLASMA MEMBRANE 0.00602238343902237 0.727272612635576 1949 Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function fungal-type vacuole YPR122W AXL1 hom FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|SITE OF POLARIZED GROWTH 0.00599704575544528 0.728374575871156 1950 Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells peptide pheromone maturation|axial cellular bud site selection metalloendopeptidase activity mating projection tip|cellular bud neck YJR033C RAV1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM 0.00594178001822681 0.730780087456431 1951 Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate early endosome to late endosome transport|vacuolar acidification|regulation of protein complex assembly molecular_function RAVE complex|cytoplasm|extrinsic to membrane YMR294W-A_d YMR294W-A hom 0.0059355560654751 0.731051159311339 1952 Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Unknown Unknown Unknown YNR007C ATG3 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.00593066891851284 0.731264032785769 1953 E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p C-terminal protein lipidation|piecemeal microautophagy of nucleus|mitochondrion degradation|CVT pathway|autophagic vacuole assembly Atg8 ligase activity cytosol|cytoplasm YMR201C RAD14 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00589717639012378 0.732723450147364 1954 Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein nucleotide-excision repair, DNA damage recognition damaged DNA binding|zinc ion binding nucleotide-excision repair factor 1 complex|nucleus YER164W CHD1 hom FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME|HISTONE ACETYLTRANSFERASE 0.0058631486003074 0.734207181053141 1955 Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes termination of RNA polymerase I transcription|ATP-dependent chromatin remodeling|nucleosome positioning|negative regulation of histone H3-K14 acetylation|termination of RNA polymerase II transcription|regulation of transcription by chromatin organization|regulation of transcriptional start site selection at RNA polymerase II promoter|nucleosome mobilization|Unknown|transcription elongation from RNA polymerase II promoter chromatin DNA binding|DNA-dependent ATPase activity|methylated histone residue binding|DNA binding|rDNA binding nuclear chromatin|SLIK (SAGA-like) complex|mitochondrion|SAGA complex|nucleolar chromatin YLR370C ARC18 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|MITOCHONDRION|CYTOSKELETON 0.00585838127254794 0.735063786764378 1956 Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin filament organization|establishment of mitochondrion localization molecular_function mitochondrion|Arp2/3 protein complex YPR046W MCM16 hom FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|KINETOCHORE 0.00583945497120202 0.73524089284712 1957 Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YNL294C RIM21 hom FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00581876559915814 0.736143926382067 1958 pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH fungal-type cell wall biogenesis|ascospore formation|invasive growth in response to glucose limitation molecular_function integral to membrane YGL048C RPT6 het FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.00578923959539127 0.737433285281503 1959 ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of sequence-specific DNA binding transcription factor activity|nucleotide-excision repair|proteasome regulatory particle assembly|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of sequence-specific DNA binding transcription factor activity|chromatin remodeling ATPase activity|protein domain specific binding proteasome regulatory particle, base subcomplex|proteasome storage granule|nucleus YKR066C CCP1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.0057873203959648 0.737517119625686 1960 Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress cellular response to oxidative stress cytochrome-c peroxidase activity mitochondrion|mitochondrial intermembrane space YPR194C OPT2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PLASMA MEMBRANE 0.0057279859984076 0.74014784800731 1961 Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles oligopeptide transport|vacuole fusion, non-autophagic oligopeptide transporter activity integral to membrane|integral to plasma membrane YER150W SPI1 hom 0.00569866321336409 0.741541986492154 1962 GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p response to acid molecular_function fungal-type cell wall YOL111C MDY2 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|NUCLEUS 0.00568868481292246 0.741829894460492 1963 Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes protein insertion into ER membrane|cell morphogenesis involved in conjugation with cellular fusion|posttranslational protein targeting to membrane molecular_function ribosome|TRC complex|nucleus|cytoplasm YBL059W_p YBL059W hom FT MITOCHONDRION 0.00568129377919398 0.74219047129176 1964 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication biological_process molecular_function mitochondrion YOR274W MOD5 hom FT RNA PROCESSING|NUCLEUS|MITOCHONDRION 0.00566552692178768 0.742843639327026 1965 Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis tRNA modification tRNA dimethylallyltransferase activity cytosol|mitochondrion|nucleolus|nucleus YGR204W ADE3 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|NUCLEUS 0.00561983324134309 0.744845202831011 1966 Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine purine nucleobase biosynthetic process|folic acid-containing compound metabolic process methylenetetrahydrofolate dehydrogenase (NADP+) activity|formate-tetrahydrofolate ligase activity|methenyltetrahydrofolate cyclohydrolase activity nucleus|cytoplasm YOL091W SPO21 hom FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON 0.00560684492777595 0.745524413345433 1967 Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation ascospore wall assembly|meiosis structural molecule activity spindle pole body YNL202W SPS19 hom FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS 0.00554868589157808 0.74796516420151 1968 Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate fatty acid catabolic process|ascospore formation 2,4-dienoyl-CoA reductase (NADPH) activity peroxisomal matrix YIR031C DAL7 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.00551377494986503 0.749678157082503 1969 Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process malate synthase activity cellular_component YHR160C PEX18 hom FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION 0.00548791326329986 0.750633445679371 1970 Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p protein import into peroxisome matrix protein binding cytosol|peroxisome YBL092W RPL32 het FF|FT TRANSLATION|RIBOSOME 0.00548096630549292 0.751190123064435 1971 Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDL103C QRI1 het FT CARBOHYDRATE METABOLISM|NUCLEUS 0.00544362599940551 0.752579802938866 1972 UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress UDP-N-acetylglucosamine biosynthetic process UDP-N-acetylglucosamine diphosphorylase activity nucleus|cytoplasm YDR469W SDC1 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS 0.0054013128638757 0.75444087035241 1973 Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 histone H3-K4 methylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YOL113W SKM1 hom FT CELL DIVISION|PROTEIN PHOSPHORYLATION|NUCLEUS|PLASMA MEMBRANE 0.00538977642646546 0.754983896192487 1974 Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth negative regulation of gene expression|cytokinetic cell separation|protein phosphorylation|sterol import protein serine/threonine kinase activity|protein kinase activity plasma membrane|nucleus YNR008W LRO1 hom FT LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.00536701654503837 0.755950379494108 1975 Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase lipid storage|triglyceride biosynthetic process phospholipid:diacylglycerol acyltransferase activity lipid particle|perinuclear endoplasmic reticulum YLR211C_p YLR211C hom 0.00531070550805851 0.758430856826512 1976 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron biological_process molecular_function cytoplasm YJR119C JHD2 hom FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEUS 0.0052555567093435 0.760862549519062 1977 JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation histone demethylation histone demethylase activity (H3-K4 specific) nucleus|cytoplasm YPR200C ARR2 hom 0.0052520749359001 0.761050701565127 1978 Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p response to arsenic-containing substance arsenate reductase activity cellular_component YPL061W ALD6 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.00524821764304303 0.761186331890601 1979 Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress response to salt stress|NADPH regeneration|acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity cytosol|mitochondrion YPL227C ALG5 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00519092335559491 0.763715454335301 1980 UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein N-linked glycosylation dolichyl-phosphate beta-glucosyltransferase activity endoplasmic reticulum membrane YCR025C_d YCR025C hom 0.00518420550439388 0.76401216390112 1981 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Unknown Unknown Unknown YPR153W YPR153W hom 0.00516177363142524 0.765003170610263 1982 Putative protein of unknown function biological_process molecular_function integral to membrane YOL012C HTZ1 hom FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00513637794822263 0.766125578277062 1983 Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin nuclear-transcribed mRNA catabolic process, non-stop decay|regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette chromatin binding nuclear chromatin|chromatin assembly complex YBR277C_d YBR277C hom 0.00513189570927471 0.766323729876926 1984 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Unknown Unknown Unknown YMR044W IOC4 hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.00512274339793481 0.766897312605456 1985 Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YMR152W YIM1 hom FT RESPONSE TO DNA DAMAGE|OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.0051148245926737 0.76707855258117 1986 Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress response to DNA damage stimulus molecular_function mitochondrion|lipid particle|cytoplasm YLR313C SPH1 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00510581085408103 0.76747719650642 1987 Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p bipolar cellular bud site selection|pseudohyphal growth|invasive filamentous growth|mating projection assembly molecular_function incipient cellular bud site|cellular bud neck|cellular bud tip|mating projection YKL112W ABF1 het FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING|NUCLEUS 0.00510151606438608 0.767667160621981 1988 DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair negative regulation of transcription from RNA polymerase II promoter|global genome nucleotide-excision repair|chromatin remodeling|DNA-dependent DNA replication|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific DNA binding, bending nucleotide-excision repair factor 4 complex|nucleus YMR243C ZRC1 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00507780065505669 0.768716374861091 1989 Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication cobalt ion transport|cellular zinc ion homeostasis|zinc ion transport|glutathione metabolic process zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity integral to membrane|fungal-type vacuole YKL007W CAP1 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00505525374578077 0.769714285313834 1990 Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YNL006W LST8 het FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.00504761883324415 0.770052287716596 1991 Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat transport|TOR signaling cascade|fungal-type cell wall organization|signal transduction|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|mitochondria-nucleus signaling pathway protein kinase activator activity|protein binding TORC1 complex|TORC2 complex|endosome membrane|extrinsic to internal side of plasma membrane|Golgi membrane YML061C PIF1 hom FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|NUCLEUS|MITOCHONDRION 0.00504621996375368 0.770280843232331 1992 DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells chromosome organization|negative regulation of telomere maintenance via telomerase|telomere maintenance|mitochondrial genome maintenance|DNA recombination DNA helicase activity|telomerase inhibitor activity mitochondrion|nucleus|mitochondrial membrane YPL144W POC4 hom FT PROTEIN COMPLEX ORG/BIOGENESIS 0.00504274312751918 0.770268161141106 1993 Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions proteasome assembly|chaperone-mediated protein complex assembly molecular_function intracellular|cytoplasm YFL016C MDJ1 hom FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00501141739252794 0.771655541743664 1994 Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones protein refolding|response to heat|misfolded or incompletely synthesized protein catabolic process|mitochondrial genome maintenance|'de novo' protein folding unfolded protein binding|ATPase activator activity extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial matrix YPL175W SPT14 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00500954146887229 0.772136104071718 1995 UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|endoplasmic reticulum YOR236W DFR1 het FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM 0.0049972104973868 0.772284989662858 1996 Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism tetrahydrofolate biosynthetic process|dihydrofolate metabolic process dihydrofolate reductase activity|mRNA binding cytosol YHR209W CRG1 hom 0.00498768039123297 0.772707312662675 1997 S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin lipid homeostasis mRNA binding|S-adenosylmethionine-dependent methyltransferase activity|methyltransferase activity cellular_component YNL268W LYP1 hom FT MITOCHONDRION|PLASMA MEMBRANE 0.00496860067517344 0.77355302507828 1998 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids basic amino acid transport basic amino acid transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YOL077C BRX1 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEUS 0.00496367049738216 0.773771599981861 1999 Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif ribosomal large subunit assembly 5S rRNA binding|rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YDL013W SLX5 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS|UBIQUITIN LIGASE COMPLEX 0.00495691804389963 0.774070992897843 2000 Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers protein sumoylation|telomere maintenance|response to DNA damage stimulus|protein ubiquitination SUMO binding|ubiquitin-protein ligase activity ubiquitin ligase complex|nucleus YMR034C_p YMR034C hom 0.00495667406833994 0.774081811010428 2001 Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process sterol transporter activity integral to membrane YNL059C ARP5 hom FT CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.00495616912518441 0.774104200818236 2002 Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes nucleosome mobilization|chromatin remodeling ATP-dependent 3'-5' DNA helicase activity nucleus|Ino80 complex YJL120W_d YJL120W hom FF 0.0049463742426909 0.774538555233768 2003 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Unknown Unknown Unknown YDR028C REG1 hom FF|FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00494060212018455 0.774794553257972 2004 Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p cellular response to glucose starvation|vacuolar protein catabolic process|glycogen metabolic process|negative regulation of transcription from RNA polymerase II promoter|regulation of carbohydrate metabolic process protein phosphatase type 1 regulator activity protein phosphatase type 1 complex|cytoplasm YNL121C TOM70 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00491870005636874 0.775766148838121 2005 Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication protein import into mitochondrial matrix|protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YDR174W HMO1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|NUCLEUS|CHROMOSOME 0.00491349063566884 0.776159894252278 2006 Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase regulation of transcription from RNA polymerase I promoter|regulation of transcription by chromatin organization|regulation of ribosomal protein gene transcription from RNA polymerase II promoter double-stranded DNA binding|four-way junction DNA binding|DNA binding, bending nuclear chromatin|nucleolus|cytoplasm YGR162W TIF4631 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|MITOCHONDRION|RIBOSOME 0.00488252487416981 0.777371680795267 2007 Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication translational initiation|ribosomal large subunit biogenesis|stress granule assembly translation initiation factor activity mitochondrion|ribosome|eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule YEL022W GEA2 hom FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GOLGI APPARATUS 0.00487961466625916 0.777500883457175 2008 Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|secretory granule organization|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|extrinsic to membrane YLR099C ICT1 hom FT KETONE METABOLISM|LIPID METABOLISM 0.00485867504520489 0.778430707712719 2009 Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication phosphatidic acid biosynthetic process lysophosphatidic acid acyltransferase activity cellular_component YJR111C_p YJR111C hom FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION 0.00485716841368491 0.77849762195883 2010 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria biological_process molecular_function mitochondrion YDR124W_p YDR124W hom 0.00483956380695166 0.779472050368941 2011 Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor biological_process molecular_function cellular_component YGL175C SAE2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00482215992428575 0.780212734909757 2012 Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents DNA double-strand break processing|DNA catabolic process, endonucleolytic|meiotic DNA double-strand break formation|meiotic DNA double-strand break processing|gene conversion at mating-type locus, DNA double-strand break processing double-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleus|cytoplasm YHR143W-A RPC10 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.00480931908641404 0.780623612514155 2013 RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YHR199C AIM46 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|MITOCHONDRION 0.00479271199940788 0.781361865267989 2014 Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YGR128C UTP8 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION|NUCLEUS|CHROMOSOME 0.00478867306051214 0.781541442380772 2015 Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|tRNA export from nucleus snoRNA binding|tRNA binding small-subunit processome|t-UTP complex|nucleolus|rDNA heterochromatin|90S preribosome YCR082W AHC2 hom FT CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.00477017117611752 0.782364211828906 2016 Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex|nucleus|cytoplasm YBL058W SHP1 hom FT PROTEIN LOCALIZATION|CELL CYCLE|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION|NUCLEUS 0.00476782275407519 0.782626814145032 2017 UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate glycogen metabolic process|proteasomal ubiquitin-dependent protein catabolic process|piecemeal microautophagy of nucleus|ER-associated protein catabolic process|ascospore formation|sister chromatid biorientation|autophagic vacuole assembly protein phosphatase type 1 regulator activity nucleus|cytoplasm YDR289C RTT103 hom FT RNA PROCESSING|NUCLEUS|CHROMOSOME 0.00475526584255389 0.783027221582774 2018 Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated|mRNA 3'-end processing RNA polymerase II core binding nuclear chromatin|site of double-strand break YBR133C HSL7 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|SITE OF POLARIZED GROWTH 0.00473928645589703 0.783738181665992 2019 Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress G2/M transition of mitotic cell cycle|regulation of cell cycle protein-arginine omega-N symmetric methyltransferase activity|protein-arginine omega-N monomethyltransferase activity cellular bud neck YHR029C YHI9 hom FT SIGNALING 0.00473179310553368 0.784291541048875 2020 Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production endoplasmic reticulum unfolded protein response molecular_function cellular_component YBL098W BNA4 hom FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|MITOCHONDRION 0.00471243280678676 0.784933369248365 2021 Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease de novo NAD biosynthetic process from tryptophan kynurenine 3-monooxygenase activity mitochondrial outer membrane|mitochondrion YLR169W_d YLR169W hom 0.00471169247787411 0.784966326599274 2022 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCR033W SNT1 hom FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|NUCLEUS|CHROMOSOME 0.00469949233862824 0.785634331144861 2023 Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance negative regulation of meiosis|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|Set3 complex YCL014W BUD3 hom FT CELL CYCLE|SIGNALING|CELL DIVISION|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00465015095702846 0.787707323286725 2024 Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding cellular bud site selection|axial cellular bud site selection|cytokinesis molecular_function cellular bud neck contractile ring|cellular bud neck YDL066W IDP1 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|MITOCHONDRION 0.00462970563648727 0.788618520793219 2025 Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes isocitrate metabolic process|glutamate biosynthetic process isocitrate dehydrogenase (NADP+) activity mitochondrial nucleoid|mitochondrion YHR030C SLT2 hom FF|FT PROTEIN LOCALIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.00462065558940634 0.789698824050691 2026 Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway protein phosphorylation|endoplasmic reticulum unfolded protein response|signal transduction|UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|regulation of transcription factor import into nucleus|barrier septum assembly|response to acid|regulation of cell size MAP kinase activity|protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm|cellular bud tip YMR272W-B_p YMR272W-B hom 0.00459849107994503 0.790010233927253 2027 Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YNL064C YDJ1 hom FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION 0.00454369459735198 0.792454973776737 2028 Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family protein refolding|ER-associated protein catabolic process|protein targeting to mitochondrion|protein targeting to ER|'de novo' protein folding unfolded protein binding|ATPase activator activity cytosol|TRC complex|perinuclear region of cytoplasm YDR159W SAC3 hom FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00451161833854219 0.794127459983575 2029 Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) ribosomal small subunit biogenesis|actin filament-based process|mRNA export from nucleus|mRNA 3'-end processing|mitosis|protein export from nucleus|transcription-coupled nucleotide-excision repair|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery protein binding transcription export complex 2|nuclear pore YMR126C_p DLT1 hom 0.00445098505167639 0.796625501312017 2030 Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) biological_process molecular_function cellular_component YDR367W KEI1 het FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.0044329467699131 0.797431736876241 2031 Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene inositolphosphoceramide metabolic process inositol phosphoceramide synthase regulator activity integral to membrane|Golgi apparatus|integral to Golgi membrane|inositol phosphoceramide synthase complex|cytoplasm YPL174C NIP100 hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.0044009552914621 0.799008983533444 2032 Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) establishment of mitotic spindle orientation microtubule binding dynactin complex|cell cortex|spindle pole body|astral microtubule YBL071C_d YBL071C hom 0.00440035270890071 0.798859753719051 2033 Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Unknown Unknown Unknown YBL083C_d YBL083C hom 0.00439690383499112 0.799014024427821 2034 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Unknown Unknown Unknown YNL324W_d YNL324W hom 0.00439469963080313 0.799112624237196 2035 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML128C MSC1 hom FT CELL CYCLE|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00436372909943374 0.800498354821976 2036 Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated reciprocal meiotic recombination molecular_function mitochondrion|endoplasmic reticulum|plasma membrane YBL049W MOH1 hom 0.00429655413674013 0.803506151480739 2037 Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase biological_process molecular_function cellular_component YJR130C STR2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.00426722054621851 0.805020199122842 2038 Cystathionine gamma-synthase, converts cysteine into cystathionine sulfur compound metabolic process|transsulfuration cystathionine gamma-synthase activity nucleus|cytoplasm YIL170W HXT12 hom 0.00426246869078731 0.805033462563977 2039 Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family hexose transport|biological_process hexose transmembrane transporter activity|molecular_function integral to membrane YPL181W CTI6 hom FT CHROMATIN ORGANIZATION|GENE SILENCING|NUCLEUS|CHROMOSOME 0.00422976018481733 0.80652805523686 2040 Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain negative regulation of chromatin silencing at silent mating-type cassette|positive regulation of transcription, DNA-dependent|regulation of DNA-dependent DNA replication initiation|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA methylated histone residue binding|transcription factor binding Rpd3L-Expanded complex|nucleus|Rpd3L complex YJR142W_p YJR142W hom 0.00422499073436607 0.806713644844018 2041 Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member biological_process molecular_function cellular_component YKL183W LOT5 hom FT NUCLEUS 0.00421999546662803 0.806965875248344 2042 Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YDR228C PCF11 het FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.00419628825422034 0.808197597310632 2043 mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping mRNA cleavage|termination of RNA polymerase II transcription|mRNA polyadenylation mRNA binding|RNA polymerase II core binding mRNA cleavage factor complex YHR189W PTH1 hom FT TRANSLATION|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00419117282353747 0.808370741017325 2044 One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium mitochondrial translation aminoacyl-tRNA hydrolase activity mitochondrion YDL207W GLE1 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|NUCLEAR PORE 0.00418657154042639 0.808436949807073 2045 Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export poly(A)+ mRNA export from nucleus|mRNA export from nucleus|regulation of translational initiation|regulation of translational termination translation initiation factor binding|inositol hexakisphosphate binding|phospholipid binding|enzyme activator activity mitochondrion|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore|cytoplasm YDL150W RPC53 het FF|FT NUCLEUS 0.00417263390043754 0.809062357168922 2046 RNA polymerase III subunit C53 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YNL243W SLA2 hom FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00416742514432733 0.809296114888247 2047 Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo bipolar cellular bud site selection|fungal-type cell wall organization|actin filament organization|endocytosis|exocytosis protein binding, bridging incipient cellular bud site|mating projection tip|actin cortical patch YNL247W YNL247W het FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION 0.00415411333603721 0.809893597317211 2048 Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments cysteinyl-tRNA aminoacylation cysteine-tRNA ligase activity ribosome|cytoplasm YGL060W YBP2 hom FT CELL CYCLE|NUCLEUS|CHROMOSOME|KINETOCHORE 0.00412480214788674 0.811209580151275 2049 Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication G2/M transition of mitotic cell cycle molecular_function condensed nuclear chromosome kinetochore|centromere-specific nucleosome|cytoplasm YDR535C_d YDR535C hom 0.00411466232454074 0.811664951622265 2050 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Unknown Unknown Unknown YMR290W-A_d YMR290W-A het FF 0.00406285956178371 0.813992363540445 2051 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Unknown Unknown Unknown YHR129C ARP1 hom FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CYTOSKELETON 0.00406115910087267 0.814068790371399 2052 Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin nuclear migration|establishment of mitotic spindle orientation structural constituent of cytoskeleton cell cortex|dynactin complex|spindle pole body|astral microtubule YDL148C NOP14 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS|MITOCHONDRION 0.0040488927491307 0.81462015114418 2053 Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit biogenesis|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing snoRNA binding small-subunit processome|mitochondrion|nucleolus|90S preribosome|Noc4p-Nop14p complex YNL098C RAS2 hom FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|RESPONSE TO NUTRIENT LEVELS|PLASMA MEMBRANE 0.00403161879975356 0.815396754239353 2054 GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes pseudohyphal growth|ascospore formation|activation of adenylate cyclase activity|regulation of protein localization|replicative cell aging|positive regulation of transcription by galactose|Ras protein signal transduction GTP binding|GTPase activity plasma membrane YLR282C_d YLR282C hom 0.00402640318316052 0.815712276278355 2055 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YGR240C PFK1 hom FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.00399284944009355 0.817220771719296 2056 Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes proton transport|glycolysis 6-phosphofructokinase activity mitochondrion|6-phosphofructokinase complex|cytoplasm YGL087C MMS2 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00398738148624775 0.817386402614573 2057 Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress postreplication repair|response to DNA damage stimulus|protein polyubiquitination|free ubiquitin chain polymerization ubiquitin-protein ligase activity ubiquitin conjugating enzyme complex|nucleus|cytoplasm YJR059W PTK2 hom FF|FT CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|NUCLEUS|PLASMA MEMBRANE 0.00397286292022805 0.818039656888748 2058 Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake protein phosphorylation|cellular ion homeostasis|polyamine transport|G1/S transition of mitotic cell cycle|regulation of cell size protein kinase activity nucleus|plasma membrane|cytoplasm YML092C PRE8 het FF|FT PROTEOLYSIS|ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION 0.00388702582529927 0.821982755660861 2059 Alpha 2 subunit of the 20S proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YBR272C HSM3 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00386447994747367 0.82318009351224 2060 Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation mismatch repair|proteasome regulatory particle assembly molecular_function cytosol|proteasome regulatory particle, base subcomplex|nucleus|cytoplasm YNL002C RLP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEUS 0.00386385194469375 0.823026445124015 2061 Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA binding nucleolus|preribosome, large subunit precursor YOR011W AUS1 hom FT NUCLEOTIDE METABOLISM|MITOCHONDRION|PLASMA MEMBRANE 0.00386372110181705 0.823006369099287 2062 Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen sterol import sterol-transporting ATPase activity mitochondrion|integral to membrane|plasma membrane YDR466W PKH3 hom FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION 0.00383758711100594 0.824132324978303 2063 Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant MAPK cascade involved in cell wall biogenesis|protein phosphorylation protein kinase activity cellular_component YNL196C_p SLZ1 hom 0.00382845756128908 0.824569632046601 2064 Sporulation-specific protein with a leucine zipper motif biological_process molecular_function cellular_component YDR452W PPN1 hom FT NUCLEUS 0.00374229010988769 0.828430784948531 2065 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress polyphosphate catabolic process|polyphosphate metabolic process endopolyphosphatase activity|exopolyphosphatase activity fungal-type vacuole membrane|nucleus YCR102C_p YCR102C hom FT OXIDATION-REDUCTION PROCESS 0.00373735926608053 0.828955431716807 2066 Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to copper ion molecular_function cellular_component YJR040W GEF1 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE 0.00372929166633583 0.829017493117037 2067 Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism cellular copper ion homeostasis|cellular iron ion homeostasis voltage-gated chloride channel activity endosome|integral to membrane|endoplasmic reticulum|Golgi apparatus|plasma membrane|fungal-type vacuole|Golgi medial cisterna YEL036C ANP1 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00372017622293066 0.829428991971097 2068 Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol protein N-linked glycosylation alpha-1,6-mannosyltransferase activity|mannosyltransferase activity alpha-1,6-mannosyltransferase complex|Golgi cis cisterna YPL131W RPL5 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RIBOSOME 0.00371310672248287 0.829998312081855 2069 Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YOL159C YOL159C hom 0.00370682355141063 0.83003185661465 2070 Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA biological_process molecular_function cellular_component YML117W NAB6 hom 0.00358076367819508 0.835897359034737 2071 Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP biological_process molecular_function cytoplasm YLR006C SSK1 hom FT SIGNALING|PROTEIN PHOSPHORYLATION 0.00353950002328776 0.837594776125506 2072 Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate activation of MAPKKK activity involved in osmosensory signaling pathway|positive regulation of protein autophosphorylation|osmosensory signaling pathway via two-component system protein kinase activator activity|two-component response regulator activity|protein homodimerization activity|mitogen-activated protein kinase kinase kinase binding cytoplasm YLR233C EST1 hom FT NUCLEUS|CHROMOSOME 0.00353632824794826 0.837738285692987 2073 TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA telomere maintenance via telomerase|telomere maintenance|G-quadruplex DNA formation single-stranded DNA binding|RNA binding|telomeric DNA binding nucleolus|nucleus|telomerase holoenzyme complex YML017W PSP2 hom 0.00353185774730344 0.837940566309413 2074 Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process molecular_function cytoplasm YPR104C FHL1 het FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS|CHROMOSOME 0.0034811157128369 0.840331309026556 2075 Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter RNA polymerase II transcription corepressor binding|RNA polymerase II transcription coactivator binding|sequence-specific DNA binding|DNA binding nuclear chromatin|nucleolus|nucleus|CURI complex YJL008C CCT8 het 0.00346672927981655 0.840888697980262 2076 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YNL183C NPR1 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|GOLGI APPARATUS|PLASMA MEMBRANE 0.00344303532005646 0.84196179423888 2077 Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex regulation of transmembrane transporter activity|regulation of nitrogen utilization|protein phosphorylation|negative regulation of endocytosis protein serine/threonine kinase activity Golgi apparatus|plasma membrane|cytoplasm YLR355C ILV5 het FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|MITOCHONDRION 0.00340685500469752 0.843600961440762 2078 Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids mitochondrial genome maintenance|branched-chain amino acid biosynthetic process double-stranded DNA binding|ketol-acid reductoisomerase activity mitochondrial nucleoid|mitochondrion YLR149C YLR149C hom 0.00340566662419334 0.843654813139461 2079 Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function cellular_component YDR403W DIT1 hom FT CELL WALL ORG/BIOGENESIS 0.0033954185556824 0.844188083248603 2080 Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure ascospore wall assembly catalytic activity cellular_component YIL048W NEO1 het FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00338880977716409 0.844418763076774 2081 Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus retrograde vesicle-mediated transport, Golgi to ER|vacuole organization|endocytosis phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism endosome|COPI-coated vesicle|integral to membrane|Golgi apparatus|Golgi membrane YBR283C SSH1 hom FT PROTEIN LOCALIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00337842248486994 0.84488958659805 2082 Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential SRP-dependent cotranslational protein targeting to membrane protein transmembrane transporter activity|signal sequence binding integral to membrane|Ssh1 translocon complex YGR241C YAP1802 hom FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON 0.00335340605986309 0.846023730029926 2083 Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YER044C ERG28 hom FT LIPID METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00334382068276995 0.846458376869457 2084 Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p ergosterol biosynthetic process protein binding, bridging endoplasmic reticulum membrane YBR002C RER2 het FF|FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00332260444067227 0.847532981713535 2085 Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting dolichol biosynthetic process|protein glycosylation|ER to Golgi vesicle-mediated transport dehydrodolichyl diphosphate synthase activity|prenyltransferase activity endoplasmic reticulum YLR040C_p YLR040C hom 0.00330728367942184 0.848137938350542 2086 Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential biological_process molecular_function fungal-type cell wall YDR369C XRS2 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.00326146015629779 0.850194908108016 2087 Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling double-strand break repair via nonhomologous end joining|meiotic DNA double-strand break formation|base-excision repair|telomere maintenance|sporulation resulting in formation of a cellular spore|double-strand break repair via break-induced replication single-stranded telomeric DNA binding|protein binding, bridging|DNA binding|double-stranded telomeric DNA binding|telomeric DNA binding|G-quadruplex DNA binding nucleus|Mre11 complex YNL197C WHI3 hom FT PROTEIN LOCALIZATION|CELL CYCLE 0.00326073553624915 0.850249866002711 2088 RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication pseudohyphal growth|invasive growth in response to glucose limitation|regulation of cell size|cytoplasmic sequestering of protein RNA binding cytoplasm YOR334W MRS2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00325561576156729 0.850460185391865 2089 Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YGR036C CAX4 hom FT LIPID METABOLISM|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00324723999359086 0.850840391289188 2090 Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation lipid biosynthetic process|protein N-linked glycosylation dolichyldiphosphatase activity integral to endoplasmic reticulum membrane|integral to membrane YOR204W DED1 het FT TRANSLATION 0.00323798465050319 0.851260564541718 2091 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility translational initiation RNA strand annealing activity|ATP-dependent RNA helicase activity cytoplasm YNL325C FIG4 hom FT LIPID METABOLISM 0.00321066292101836 0.852501159797799 2092 Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity fungal-type vacuole membrane|PAS complex|extrinsic to membrane YKL033W TTI1 het FT CHROMATIN ORGANIZATION|NUCLEUS|MITOCHONDRION|CHROMOSOME 0.00317866293514344 0.853976175785139 2093 Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ASTRA complex|mitochondrion|cytoplasm YBR148W YSW1 hom FT MEMBRANE ORGANIZATION 0.00315382495080413 0.855083161785308 2094 Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane ascospore-type prospore membrane assembly molecular_function prospore membrane YBL036C YBL036C hom 0.00314386200041836 0.855535913477963 2095 Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS biological_process pyridoxal phosphate binding cellular_component YDL200C MGT1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00312971693245134 0.85624244075342 2096 DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage DNA dealkylation involved in DNA repair methylated-DNA-[protein]-cysteine S-methyltransferase activity nucleus YMR123W PKR1 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00311045394764926 0.857582542303958 2097 V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress vacuolar proton-transporting V-type ATPase complex assembly molecular_function integral to endoplasmic reticulum membrane YNL248C RPA49 hom FT NUCLEUS 0.00310279663016455 0.857402569293221 2098 RNA polymerase I subunit A49 transcription elongation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of cell size molecular_function DNA-directed RNA polymerase I complex YER082C UTP7 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00309929834272079 0.8575826352866 2099 Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YOR012W_p YOR012W hom 0.00307983617574037 0.858530157086602 2100 Putative protein of unknown function biological_process molecular_function cellular_component YDR464W SPP41 het FT TRANSCRIPTION FROM RNA POL II|NUCLEUS 0.00305183887129633 0.85974069849428 2101 Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 negative regulation of transcription from RNA polymerase II promoter molecular_function nucleus YDR081C PDC2 het FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00300380267288337 0.861905668944454 2102 Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions positive regulation of thiamine biosynthetic process|Unknown|positive regulation of transcription from RNA polymerase II promoter transcription regulatory region sequence-specific DNA binding nucleus YLR403W SFP1 hom FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|NUCLEUS 0.00298026121936569 0.862977203758599 2103 Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|regulation of cell size|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription factor binding transcription factor activity nucleus|cytoplasm YIL108W YIL108W hom FT PROTEOLYSIS 0.00296781915452693 0.86354363034124 2104 Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cytoplasm YKL057C NUP120 hom FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS|NUCLEAR PORE 0.00296364594855943 0.863733631789863 2105 Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 double-strand break repair|protein export from nucleus|ribosomal large subunit export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|telomere tethering at nuclear periphery|nuclear pore distribution|mRNA export from nucleus in response to heat stress|mRNA export from nucleus|positive regulation of transcription, DNA-dependent|negative regulation of transcription from RNA polymerase II promoter|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore outer ring YLR322W_d VPS65 hom 0.00293492680017047 0.865480577141846 2106 Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Unknown Unknown Unknown YCR030C SYP1 hom FT CYTOSKELETON ORGANIZATION|SITE OF POLARIZED GROWTH 0.00292847404051965 0.865335281732534 2107 Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization septin cytoskeleton organization|actin cortical patch assembly molecular_function mating projection base|mating projection tip|cellular bud neck|cellular bud tip YGL026C TRP5 hom FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEUS 0.00292804426633464 0.865354856120195 2108 Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis tryptophan biosynthetic process tryptophan synthase activity nucleus|cytoplasm YML054C CYB2 hom FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|MITOCHONDRION 0.0028895959798772 0.867106344871891 2109 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions lactate metabolic process L-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial intermembrane space YML052W SUR7 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|MITOCHONDRION|PLASMA MEMBRANE 0.00284212736137795 0.869269648492534 2110 Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants endocytosis|ascospore formation molecular_function cell cortex|eisosome|mitochondrion|membrane raft|integral to membrane|plasma membrane|actin cortical patch YLR423C ATG17 hom FT MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION 0.00283558478339524 0.86956789272928 2111 Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells piecemeal microautophagy of nucleus|mitochondrion degradation|peroxisome degradation|activation of protein kinase activity|autophagic vacuole assembly protein kinase activator activity|protein complex scaffold pre-autophagosomal structure|Atg1p signaling complex YOR216C RUD3 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|GOLGI APPARATUS 0.00283355436939449 0.869660453178894 2112 Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus YDR108W TRS85 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION|GOLGI APPARATUS 0.00280590768960832 0.870920959024881 2113 Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role meiosis|vesicle organization|piecemeal microautophagy of nucleus|ER to Golgi vesicle-mediated transport|peroxisome degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway molecular_function TRAPP complex|pre-autophagosomal structure YOR152C_p YOR152C hom 0.00277435663658562 0.872359878824041 2114 Putative protein of unknown function; YOR152C is not an essential gene biological_process molecular_function membrane YLR420W URA4 hom FT NUCLEOTIDE METABOLISM|NUCLEUS 0.00277012389478994 0.872552949805469 2115 Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine nucleotide biosynthetic process dihydroorotase activity nucleus|cytoplasm YKR024C DBP7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00274799320418519 0.873656036222283 2116 Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YOL141W PPM2 hom FT RNA PROCESSING|MITOCHONDRION 0.00274002809504229 0.87392594848518 2117 AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p tRNA methylation|wybutosine biosynthetic process tRNA methyltransferase activity mitochondrion|cytoplasm YPR133C SPN1 het FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RNA LOCALIZATION|NUCLEUS 0.0027362741357363 0.874097234347 2118 Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype poly(A)+ mRNA export from nucleus|regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription by chromatin organization|regulation of transcription from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity nucleus YDR076W RAD55 hom FT CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEUS 0.002732985716394 0.874247283371996 2119 Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p DNA recombinase assembly|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YER077C_p YER077C hom FT MITOCHONDRION 0.00271230477438327 0.875191048404359 2120 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function mitochondrion YDR293C SSD1 hom FF|FT CELL CYCLE|TRANSLATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|RNA LOCALIZATION|NUCLEUS|SITE OF POLARIZED GROWTH 0.00269189251898342 0.876544845308043 2121 Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function regulation of fungal-type cell wall organization|negative regulation of translation|intracellular mRNA localization mRNA binding|mRNA 5'-UTR binding|translation repressor activity, nucleic acid binding nucleus|cellular bud|cytoplasmic stress granule|cytoplasmic mRNA processing body|cellular bud neck|cytoplasm YFL025C BST1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|PROTEOLYSIS|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00266898103501089 0.877368702461716 2122 GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules protein retention in ER lumen|vesicle organization|ER-associated protein catabolic process|ER to Golgi vesicle-mediated transport|GPI anchor metabolic process phosphatidylinositol deacylase activity integral to membrane|endoplasmic reticulum YGL013C PDR1 hom FF|FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RSC COMPLEX 0.00266666186119794 0.877274567384053 2123 Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Unknown|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YNL246W VPS75 hom FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|NUCLEUS|CHROMOSOME 0.00264562356112368 0.87823521603134 2124 NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress double-strand break repair via nonhomologous end joining|nucleosome assembly|positive regulation of histone acetylation histone binding|acetyltransferase activator activity chromatin|nucleus YDR277C MTH1 hom FT SIGNALING 0.00261388490739789 0.879791663716796 2125 Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation glucose transport|signal transduction molecular_function cellular_component YIL014W MNT3 hom FT CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00261072032876033 0.879829358451424 2126 Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation protein O-linked glycosylation alpha-1,3-mannosyltransferase activity cellular_component YPL216W_p YPL216W hom FT CHROMATIN ORGANIZATION|NUCLEUS 0.00260326936317835 0.880169731209119 2127 Putative protein of unknown function; YPL216W is not an essential gene biological_process molecular_function cellular_component YDL109C_p YDL109C hom FT LIPID METABOLISM 0.00258723606609319 0.880902234410219 2128 Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication cellular lipid metabolic process molecular_function cellular_component YDR264C AKR1 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00258520056624901 0.881153846037744 2129 Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication protein targeting to membrane|regulation of endocytosis|regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus|membrane YHR008C SOD2 hom FF|FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|MITOCHONDRION 0.00257573641787376 0.881462776089799 2130 Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated reactive oxygen species metabolic process|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|age-dependent response to reactive oxygen species involved in chronological cell aging superoxide dismutase activity mitochondrion|mitochondrial matrix YPL084W BRO1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS 0.00256250398914169 0.882032352847237 2131 Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes protein deubiquitination|vacuolar transport|protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|response to nutrient|ubiquitin-dependent protein catabolic process ubiquitin-specific protease activity endosome|cytoplasm YDL183C YDL183C hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00251827772156985 0.884139702874949 2132 Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene potassium ion transport|proton transport molecular_function integral to mitochondrial inner membrane YDR257C RKM4 hom FT NUCLEUS 0.00250061535965432 0.884861366379164 2133 Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity nucleus YBL093C ROX3 hom FT TRANSCRIPTION FROM RNA POL II|NUCLEUS|RNA POL II, HOLOENZYME 0.0024973984722427 0.88509361655908 2134 Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme negative regulation of transcription from RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription factor recruiting transcription factor activity core mediator complex|nucleus YOR030W DFG16 hom FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.00248449740954998 0.885683112832223 2135 Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p pseudohyphal growth|protein processing molecular_function integral to membrane YCR009C RVS161 hom FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00247781704478294 0.885937675544899 2136 Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress conjugation with cellular fusion|actin cytoskeleton reorganization|endocytosis|actin cortical patch localization|response to starvation|response to osmotic stress|lipid tube assembly|actin cytoskeleton organization cytoskeletal protein binding|lipid binding membrane raft|mating projection tip|mating projection|actin cortical patch YGR098C ESP1 het FT CELL CYCLE|LIPID METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|MITOCHONDRION|CYTOSKELETON 0.00246212790703914 0.886621422813501 2137 Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress regulation of exit from mitosis|regulation of mitotic spindle elongation|negative regulation of protein phosphatase type 2A activity|apoptotic process|mitotic sister chromatid segregation cysteine-type endopeptidase activity mitochondrion|nucleus|cytoplasm|spindle YIL053W RHR2 hom FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|NUCLEUS 0.00243194004222545 0.888002325376626 2138 Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication glycerol biosynthetic process|response to osmotic stress glycerol-1-phosphatase activity nucleus|cytoplasm YKL135C APL2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE 0.00241859240676643 0.888613003297412 2139 Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YGL219C MDM34 hom FT MITOCHONDRION ORGANIZATION|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 0.00240106725281786 0.889414910366177 2140 Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth mitochondrion organization|phospholipid transport molecular_function mitochondrial outer membrane|ERMES complex|mitochondrion|cytoplasm YDL235C YPD1 het FT SIGNALING|NUCLEUS 0.00238486161435977 0.890156540397426 2141 Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus osmosensory signaling pathway via two-component system protein histidine kinase binding|transferase activity, transferring phosphorus-containing groups nucleus|cytoplasm YAL066W_d YAL066W hom 0.00238466329006866 0.890279583422775 2142 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR048C_d YDR048C hom 0.00224156719098509 0.896718331956593 2143 Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL051W COG5 hom FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00222106627892379 0.897794374607156 2144 Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YDR168W CDC37 het FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION 0.00221081947522574 0.898127279085691 2145 Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding regulation of stress-activated MAPK cascade|regulation of cell cycle|MAPK cascade involved in osmosensory signaling pathway|protein stabilization|spindle pole body duplication associated with nuclear envelope unfolded protein binding cytoplasm YGR114C_d YGR114C het FF 0.00220418235617393 0.898642539385418 2146 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Unknown Unknown Unknown YJL175W_d YJL175W hom 0.00214687843327429 0.901102299651853 2147 Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Unknown Unknown Unknown YOR095C RKI1 het FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS|NUCLEUS 0.00214343072104875 0.901216338915442 2148 Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis pyridoxine biosynthetic process|pentose-phosphate shunt ribose-5-phosphate isomerase activity nucleus|cytoplasm YBR200W BEM1 hom FT SITE OF POLARIZED GROWTH|CYTOSKELETON 0.00213093089953422 0.90178948905554 2149 Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p establishment of cell polarity|cell morphogenesis involved in conjugation with cellular fusion protein complex scaffold|phosphatidylinositol-3-phosphate binding incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip YAL061W_p BDH2 hom FT OXIDATION-REDUCTION PROCESS|NUCLEUS 0.00212069737072415 0.902258761746065 2150 Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 biological_process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor nucleus|cytoplasm YOR070C GYP1 hom FF|FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|MITOCHONDRION|GOLGI APPARATUS 0.00211171354068496 0.902714083938455 2151 Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion vesicle-mediated transport Rab GTPase activator activity mitochondrion|Golgi apparatus YNL067W RPL9B hom FT TRANSLATION|RIBOSOME 0.00210509007790811 0.902974521537291 2152 Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YDR107C TMN2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT 0.00210246209067088 0.903095050375189 2153 Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments cellular copper ion homeostasis|pseudohyphal growth|vacuolar transport|invasive growth in response to glucose limitation molecular_function fungal-type vacuole membrane|integral to membrane YPL041C_p YPL041C hom 0.00208401875479579 0.904040812484929 2154 Protein of unknown function involved in maintenance of proper telomere length biological_process molecular_function cellular_component YNL147W LSM7 hom FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00207367511639475 0.904415468916864 2155 Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|maturation of SSU-rRNA RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex|cytoplasm YLR372W SUR4 hom FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM|ENDOPLASMIC RETICULUM 0.00207351501968113 0.904422813073048 2156 Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis post-Golgi vesicle-mediated transport|fatty acid elongation|fatty acid biosynthetic process|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum YBL017C PEP1 hom FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|GOLGI APPARATUS 0.00202596461821563 0.906604463365465 2157 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments vacuolar transport|protein targeting to vacuole signal sequence binding integral to membrane|Golgi apparatus YCR021C HSP30 hom FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|PLASMA MEMBRANE 0.00201388473200498 0.907158811049863 2158 Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase negative regulation of ATPase activity|response to stress molecular_function integral to membrane|plasma membrane YDL208W NHP2 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00200413893923942 0.907606079817205 2159 Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing cleavage involved in rRNA processing|rRNA pseudouridine synthesis|snRNA pseudouridine synthesis snoRNA binding box H/ACA snoRNP complex YPR180W AOS1 het FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00200245800026349 0.907957695460736 2160 Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity nucleus|SUMO activating enzyme complex YOR039W CKB2 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00199506881233297 0.90809065585861 2161 Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase inhibitor activity|protein kinase regulator activity protein kinase CK2 complex|UTP-C complex|CURI complex YFR039C_p YFR039C hom 0.00199272642111183 0.908129877698338 2162 Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces biological_process molecular_function cellular_component YDR165W TRM82 hom FT RNA PROCESSING|NUCLEUS 0.00198821424379593 0.908336983154079 2163 Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YCL037C SRO9 hom FT TRANSLATION 0.00197658652700471 0.908870715049524 2164 Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication cytoplasmic translation RNA binding polysome YOR117W RPT5 het FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|NUCLEUS 0.00193246875520829 0.911002070118402 2165 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YGR018C_p YGR018C hom 0.00193036262234909 0.910992874872528 2166 Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W biological_process molecular_function cellular_component YLR390W-A CCW14 hom FT CELL WALL ORG/BIOGENESIS|MITOCHONDRION 0.00191302998365679 0.911841197809782 2167 Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall fungal-type cell wall organization structural constituent of cell wall mitochondrion|fungal-type cell wall YDR414C ERD1 hom FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 0.00188478034837527 0.913086198884441 2168 Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) protein retention in ER lumen|protein glycosylation molecular_function integral to membrane|membrane YBL081W_p YBL081W hom 0.00187916905330107 0.913537193149317 2169 Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport biological_process molecular_function cellular_component YIL042C PKP1 hom FT SIGNALING|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|MITOCHONDRION 0.00187572625490193 0.913553471633375 2170 Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p peptidyl-serine phosphorylation|protein phosphorylation|carbon utilization protein kinase activity|pyruvate dehydrogenase (acetyl-transferring) kinase activity mitochondrion YGR097W ASK10 hom FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|RNA POL II, HOLOENZYME 0.00186933802086321 0.913795511021669 2171 Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication cellular protein catabolic process|cellular response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter|positive regulation of glycerol transport molecular_function DNA-directed RNA polymerase II, holoenzyme|cytoplasm YDL020C RPN4 hom FF|FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|NUCLEUS 0.00185240042358321 0.91471326958188 2172 Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress positive regulation of proteasomal ubiquitin-dependent protein catabolic process|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|regulation of DNA repair|negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YOL154W ZPS1 hom 0.00183377461438369 0.915429304426903 2173 Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH biological_process molecular_function fungal-type cell wall|fungal-type vacuole YJR137C MET5 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS 0.00182663308597625 0.915820023325083 2174 Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine fungal-type cell wall organization|sulfate assimilation sulfite reductase (NADPH) activity sulfite reductase complex (NADPH)|cytoplasm YOR260W GCD1 het FT TRANSLATION 0.00182195333819183 0.915984939640866 2175 Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation translation initiation factor activity|guanyl-nucleotide exchange factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YNL303W_d YNL303W hom 0.00181677407242189 0.916310075680643 2176 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL073C YOL073C hom 0.00180933995823851 0.916552047183525 2177 Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 biological_process molecular_function integral to membrane YCL023C_d YCL023C hom 0.00177591069158755 0.918088350468934 2178 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Unknown Unknown Unknown YKL122C SRP21 het FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEUS 0.00177466963599327 0.918145391377313 2179 Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding nucleus|signal recognition particle, endoplasmic reticulum targeting YMR074C YMR074C hom FT NUCLEUS 0.00176681084534829 0.918506603781788 2180 Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress apoptotic process molecular_function nucleus|cytoplasm YKL220C FRE2 hom FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|PLASMA MEMBRANE 0.00176505126952526 0.918587481205058 2181 Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YLR174W IDP2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM 0.00175902061576776 0.918900855674216 2182 Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication glutamate biosynthetic process|isocitrate metabolic process isocitrate dehydrogenase (NADP+) activity cytosol YPR143W RRP15 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00175720575432846 0.918948103949522 2183 Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|preribosome, large subunit precursor YGR040W KSS1 hom FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00175432770458278 0.919080399022789 2184 Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains pheromone-dependent signal transduction involved in conjugation with cellular fusion|signal transduction involved in filamentous growth|negative regulation of sequence-specific DNA binding transcription factor activity|protein phosphorylation|invasive growth in response to glucose limitation MAP kinase activity|protein kinase activity|transcription factor binding nucleus YGR251W NOP19 het FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00173264048918115 0.920077365199156 2185 Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|nucleolus|nucleus|90S preribosome YGR160W_d YGR160W hom 0.00173189728069669 0.92011153289499 2186 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR088W CTT1 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS 0.00171912877700975 0.920698565242869 2187 Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide response to reactive oxygen species catalase activity cytoplasm YOR087W YVC1 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS 0.00171035575712534 0.921101930154798 2188 Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock cellular cation homeostasis voltage-gated ion channel activity|calcium activated cation channel activity|sodium channel activity|calcium channel activity|potassium channel activity fungal-type vacuole membrane|fungal-type vacuole YNL114C_d YNL114C het 0.00171017612069577 0.921110189670475 2189 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Unknown Unknown Unknown YGR022C_d YGR022C hom 0.00169579677330715 0.921771366181212 2190 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W Unknown Unknown Unknown YPR062W FCY1 hom FT NUCLEUS 0.00168051185796073 0.922474240842567 2191 Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) cytosine metabolic process|pyrimidine-containing compound salvage|cytidine metabolic process cytosine deaminase activity nucleus|cytoplasm YDR517W GRH1 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|GOLGI APPARATUS 0.00166686715940183 0.923101741512157 2192 Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress protein secretion|ER to Golgi vesicle-mediated transport|Golgi organization molecular_function COPII vesicle coat|cis-Golgi network|cytoplasm YIL010W DOT5 hom FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS|NUCLEUS|CHROMOSOME 0.00166375402785859 0.923244916809757 2193 Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth cellular response to oxidative stress|cell redox homeostasis thioredoxin peroxidase activity nucleus YBR090C_p YBR090C hom FT NUCLEUS 0.00165784526927548 0.923596293526721 2194 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YOL119C MCH4 hom 0.00165561030305757 0.923619464678623 2195 Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport transport monocarboxylic acid transmembrane transporter activity|transporter activity fungal-type vacuole membrane|integral to membrane YDR139C RUB1 hom 0.00163895571058901 0.924385498746073 2196 Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) protein neddylation protein tag cytoplasm YIL114C POR2 hom FT ION HOMEOSTASIS|ION HOMEOSTASIS|MITOCHONDRION 0.00162863872219988 0.924860067601488 2197 Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication DNA transport|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to membrane YNL263C YIF1 het FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS 0.00162778141742728 0.924899503778306 2198 Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 ER to Golgi vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|endoplasmic reticulum membrane|ER to Golgi transport vesicle YLR200W YKE2 hom FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|NUCLEUS 0.00162730550534645 0.924921395900966 2199 Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding|tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YLR055C SPT8 hom FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|NUCLEUS|HISTONE ACETYLTRANSFERASE 0.00161438600942548 0.925515718902808 2200 Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters histone acetylation|negative regulation of transcription from RNA polymerase II promoter|chromatin modification|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|transcription cofactor activity nucleus|SAGA complex YHR195W NVJ1 hom FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|ENDOMEMBRANE SYSTEM|NUCLEUS 0.00161357049224848 0.9255532357438 2201 Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) microautophagy|cellular protein localization protein binding nuclear envelope YML065W ORC1 het FT GENE SILENCING|NUCLEUS|CHROMOSOME 0.00157070398981815 0.927525486170971 2202 Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding|ATPase activity|ATP binding DNA replication preinitiation complex|nucleus|pre-replicative complex|nuclear origin of replication recognition complex YFR025C HIS2 hom FT KETONE METABOLISM|AMINO ACID METABOLISM 0.00156499781966878 0.927809533569498 2203 Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control histidine biosynthetic process histidinol-phosphatase activity intracellular YGR023W MTL1 hom FT CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS|PLASMA MEMBRANE 0.00156121750666539 0.928047755037493 2204 Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p cellular response to glucose starvation|fungal-type cell wall organization|cellular response to oxidative stress molecular_function integral to plasma membrane YJL058C BIT61 hom FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PLASMA MEMBRANE 0.00154528335828733 0.928695278325731 2205 Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|plasma membrane|cytoplasm YBL086C_p YBL086C hom 0.00154009931591696 0.9289338536291 2206 Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YGL019W CKB1 hom FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|NUCLEUS 0.00153403827771814 0.9292549159683 2207 Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein kinase regulator activity|protein serine/threonine kinase inhibitor activity protein kinase CK2 complex|UTP-C complex YDR280W RRP45 het FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEUS 0.00152049882790836 0.929909025681923 2208 Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|U1 snRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YJL171C YJL171C hom FT MITOCHONDRION|PLASMA MEMBRANE 0.00151834062999264 0.929935281560789 2209 GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion|fungal-type cell wall|plasma membrane YML108W YML108W hom FT NUCLEUS 0.00151294686424423 0.93018354298005 2210 Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL081W_p YGL081W hom 0.00149508256648855 0.931005839863125 2211 Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis biological_process molecular_function cellular_component YPL115C BEM3 hom FT SIGNALING|SITE OF POLARIZED GROWTH 0.00149264399679564 0.931118093401005 2212 Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly negative regulation of Rho protein signal transduction|establishment of cell polarity Rho GTPase activator activity|phosphatidylinositol-3-phosphate binding cell cortex|incipient cellular bud site|mating projection tip|cytoplasm|cellular bud tip YKL110C KTI12 hom FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|NUCLEUS 0.00148373508081082 0.931558763592756 2213 Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p regulation of transcription from RNA polymerase II promoter|cell growth|tRNA wobble uridine modification chromatin binding nucleus|cytoplasm YKR037C SPC34 het FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION|NUCLEUS|CHROMOSOME|CYTOSKELETON|KINETOCHORE 0.00147023091739053 0.932260990721668 2214 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|DASH complex|condensed nuclear chromosome kinetochore|spindle pole body|spindle YNL218W MGS1 hom FT RESPONSE TO DNA DAMAGE|NUCLEUS 0.00145561349992207 0.932822867961585 2215 Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress regulation of DNA repair|DNA replication, Okazaki fragment processing|DNA strand renaturation ubiquitin binding|single-stranded DNA-dependent ATPase activity|enzyme activator activity nucleus|cytoplasm YFR016C YFR016C hom 0.00145277854121669 0.932953393869226 2216 Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene biological_process molecular_function cellular bud|cytoplasm YGR209C TRX2 hom FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS|ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS 0.00145209773957298 0.932984739304126 2217 Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress vacuole inheritance|reg