strainid orf gene zyg qualifier SGTC_1774|st048371.score SGTC_1774|st048371.expt.zyg.pval SGTC_1774|st048371.expt.zyg.significant SGTC_1774|st048371.expt.zyg.rank strain.pval strain.rank clearance Fclass Fterms.BP Fterms.CC Fterms.complex description GO_process GO_function GO_component YJL080C YJL080C SCP160 hom Verified 9.6555883539639 2.3273977136996e-22 yes 1 3.8592182825901e-23 3 NA FT CELL CYCLE|SIGNALING|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins meiotic telomere clustering|pheromone-dependent signal transduction involved in conjugation with cellular fusion|chromosome segregation|intracellular mRNA localization|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere G-protein alpha-subunit binding|RNA binding endoplasmic reticulum membrane|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YBR021W YBR021W FUR4 hom Verified 8.39037093300653 2.42305349093452e-17 yes 2 6.72184088774306e-12 22 NA FF|FT PLASMA MEMBRANE Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation uracil transport|transmembrane transport uracil:cation symporter activity integral to membrane|membrane raft|plasma membrane YNL283C YNL283C WSC2 hom Verified 7.10011002891397 6.23288019955924e-13 yes 3 1.2100574391367e-10 7 NA FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response fungal-type cell wall organization|response to heat|Rho protein signal transduction transmembrane signaling receptor activity site of polarized growth|cellular bud|mating projection tip|cytoplasm YDL118W_d YDL118W YDL118W hom Dubious 7.00179558477925 1.26351303733983e-12 yes 4 7.00695133984015e-10 20 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein biological_process molecular_function cellular_component YPL260W YPL260W YPL260W hom Verified 5.62681462376405 9.1783907663282e-09 yes 5 1.22044864136131e-07 4 NA FT NUCLEUS Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YGL221C YGL221C NIF3 hom Verified 4.72442235931362 1.15385179245946e-06 yes 6 3.38403959512645e-07 7 NA FT MITOCHONDRION Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion|cytoplasm YJR148W YJR148W BAT2 hom Verified 4.53089031969192 2.93678141044083e-06 yes 7 7.08888583531477e-07 4 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase branched-chain amino acid catabolic process|branched-chain amino acid biosynthetic process branched-chain-amino-acid transaminase activity nucleus|cytoplasm YPL032C YPL032C SVL3 hom Verified 4.44386323242569 4.41788922627249e-06 yes 8 3.68009355211058e-05 11 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS SITE OF POLARIZED GROWTH Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress endocytosis molecular_function cellular bud|cellular bud neck|cytoplasm YER090W YER090W TRP2 hom Verified 4.3968173355835 5.49248572418941e-06 yes 9 0.0275779529618478 341 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p tryptophan biosynthetic process anthranilate synthase activity anthranilate synthase complex|cytoplasm YNL072W YNL072W RNH201 hom Verified 4.33369720245905 7.33129077865017e-06 yes 10 9.81604276695774e-05 9 NA FT NUCLEUS Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome DNA replication, removal of RNA primer ribonuclease H activity ribonuclease H2 complex|nucleus YNR040W_p YNR040W YNR040W hom Uncharacterized 4.32284708150827 7.70142487827945e-06 yes 11 1.2827360002807e-05 23 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOR258W YOR258W HNT3 hom Verified 4.10830409994368 1.99287538898025e-05 yes 12 0.00016166400400229 41 NA FT RESPONSE TO DNA DAMAGE NUCLEUS DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress response to DNA damage stimulus DNA 5'-adenosine monophosphate hydrolase activity nucleus|cytoplasm YHR115C YHR115C DMA1 hom Verified 4.07938998006831 2.2577016741241e-05 yes 13 4.18482336616301e-05 8 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger establishment of mitotic spindle orientation|protein autoubiquitination|septin ring assembly|mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity cytoplasm YGR252W YGR252W GCN5 hom Verified 4.07592923301353 2.29154782475386e-05 yes 14 0.00160290090849896 58 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME HISTONE ACETYLTRANSFERASE Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation histone acetylation|regulation of transcription by chromatin organization|chromatin modification|positive regulation of transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|transcription coactivator activity|histone acetyltransferase activity|H3 histone acetyltransferase activity SLIK (SAGA-like) complex|chromosome, centromeric region|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YDR469W YDR469W SDC1 hom Verified 4.0367935644574 2.70933547041566e-05 yes 15 0.000140777787797585 22 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 histone H3-K4 methylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YER185W YER185W PUG1 hom Verified 3.9810304965609 3.43085720676609e-05 yes 16 4.21171829602952e-05 17 NA FT PLASMA MEMBRANE Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins heme transport molecular_function integral to membrane|plasma membrane YER103W YER103W SSA4 hom Verified 3.89199875019988 4.97108786413003e-05 yes 17 1.47328225006855e-05 14 NA FT PROTEIN LOCALIZATION NUCLEUS Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication protein folding|SRP-dependent cotranslational protein targeting to membrane, translocation|response to stress unfolded protein binding|ATPase activity nucleus|cytoplasm YEL031W YEL031W SPF1 hom Verified 3.86883091077157 5.46792225472465e-05 yes 18 0.000915277494488169 100 NA FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 cellular calcium ion homeostasis|transmembrane transport ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|endoplasmic reticulum membrane|cis-Golgi network YJL105W YJL105W SET4 hom Verified 3.84168690216713 6.10958201767697e-05 yes 19 8.81153124927132e-05 33 NA FT CHROMATIN ORGANIZATION Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication biological_process molecular_function cellular_component YJR059W YJR059W PTK2 hom Verified 3.82526424674489 6.53159460875062e-05 yes 20 0.000879117016853435 60 NA FF|FT CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS NUCLEUS|PLASMA MEMBRANE Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake protein phosphorylation|cellular ion homeostasis|polyamine transport|G1/S transition of mitotic cell cycle|regulation of cell size protein kinase activity nucleus|plasma membrane|cytoplasm YNR075W YNR075W COS10 hom Verified 3.80771892052532 7.0127279853009e-05 yes 21 3.51899131913833e-05 6 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins endocytosis molecular_function nuclear envelope|integral to membrane|endoplasmic reticulum|cytoplasm YDR033W YDR033W MRH1 hom Verified 3.80641945857499 7.04965894696027e-05 yes 22 6.97841361825425e-05 5 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication biological_process molecular_function mitochondrion|integral to membrane|plasma membrane YPL269W YPL269W KAR9 hom Verified 3.80333394926551 7.13808491192207e-05 yes 23 0.000295877418352951 41 NA FT NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase nuclear migration along microtubule|establishment of spindle localization molecular_function cell cortex|spindle pole body|mating projection tip YKL211C YKL211C TRP3 hom Verified 3.76598151879032 8.29480319204362e-05 yes 24 0.060942915975473 479 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p tryptophan biosynthetic process indole-3-glycerol-phosphate synthase activity|anthranilate synthase activity anthranilate synthase complex|cytoplasm YDR293C YDR293C SSD1 hom Verified 3.68028874564406 0.000116484999287697 yes 25 0.0131616545936247 204 NA FF|FT CELL CYCLE|TRANSLATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|RNA LOCALIZATION NUCLEUS|SITE OF POLARIZED GROWTH Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function regulation of fungal-type cell wall organization|negative regulation of translation|intracellular mRNA localization mRNA binding|mRNA 5'-UTR binding|translation repressor activity, nucleic acid binding nucleus|cellular bud|cytoplasmic stress granule|cytoplasmic mRNA processing body|cellular bud neck|cytoplasm YDL071C_d YDL071C YDL071C hom Dubious 3.65412501610632 0.000129030256681764 yes 26 0.000379568221459532 21 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Unknown Unknown Unknown YLR131C YLR131C ACE2 hom Verified 3.65271779167524 0.000129739677112638 yes 27 0.00204632796010906 150 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION NUCLEUS Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YOL062C YOL062C APM4 hom Verified 3.626828885743 0.00014346164862918 yes 28 3.28212046053876e-05 14 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|PLASMA MEMBRANE Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport intracellular protein transport|vesicle-mediated transport molecular_function AP-2 adaptor complex YNL259C YNL259C ATX1 hom Verified 3.55499855968888 0.000188990579996698 yes 29 0.000265698678150458 42 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake copper ion transport|cellular response to oxidative stress|cellular iron ion homeostasis copper chaperone activity cytosol YGR056W YGR056W RSC1 hom Verified 3.55181153634325 0.000191294392381671 yes 30 0.00345432213777061 84 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook double-strand break repair|ATP-dependent chromatin remodeling|regulation of sporulation resulting in formation of a cellular spore|double-strand break repair via nonhomologous end joining|nucleosome disassembly|transcription elongation from RNA polymerase II promoter DNA-dependent ATPase activity RSC complex YCL030C YCL030C HIS4 hom Verified 3.54390150990536 0.000197126247335031 yes 31 0.000212977320018776 21 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis histidine biosynthetic process phosphoribosyl-ATP diphosphatase activity|phosphoribosyl-AMP cyclohydrolase activity|histidinol dehydrogenase activity intracellular YOR246C YOR246C ENV9 hom Verified 3.5264795625185 0.00021056183307327 yes 32 0.000392727676962731 86 NA FF|FT OXIDATION-REDUCTION PROCESS|VACUOLAR PROTEIN PROCESSING Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts vacuole organization|vacuolar protein processing oxidoreductase activity lipid particle YNL093W YNL093W YPT53 hom Verified 3.52623531250457 0.000210756134506668 yes 33 0.000430502066399474 44 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT PLASMA MEMBRANE Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication endocytosis|protein targeting to vacuole GTPase activity late endosome YOR379C_d YOR379C YOR379C hom Dubious 3.49850994032367 0.000233932824545413 yes 34 0.000192854264331421 15 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Unknown Unknown Unknown YBR281C YBR281C DUG2 hom Verified 3.47810743412989 0.000252483745540954 yes 35 0.000227385455399527 38 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS NUCLEUS Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process peptidase activity|gamma-glutamyltransferase activity|omega peptidase activity nucleus|cytoplasm YGR212W YGR212W SLI1 hom Verified 3.4708466024979 0.000259410145305119 yes 36 0.000317927794070348 14 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin response to drug N-acetyltransferase activity nuclear envelope|plasma membrane YGR037C YGR037C ACB1 hom Verified 3.44774412503541 0.000282644645818439 yes 37 0.00652560159548897 155 NA FF|FT KETONE METABOLISM|LIPID METABOLISM NUCLEUS Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress long-chain fatty acid transport|chronological cell aging|fatty acid metabolic process long-chain fatty acid transporter activity|fatty-acyl-CoA binding extracellular region|nucleus|cytoplasm YFL010W-A YFL010W-A AUA1 hom Verified 3.44165441762249 0.000289084185357448 yes 38 0.00046293371805504 26 NA Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease amino acid transport molecular_function cellular_component YOL145C YOL145C CTR9 hom Verified 3.43560339227651 0.000295617906423499 yes 39 0.000868558508568285 50 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|GENE SILENCING NUCLEUS|CHROMOSOME RNA POL II, HOLOENZYME Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats transcription elongation from RNA polymerase I promoter|mRNA 3'-end processing|regulation of chromatin silencing at telomere|snoRNA transcription from an RNA polymerase II promoter|regulation of transcription by chromatin organization|transcription from RNA polymerase I promoter|regulation of histone H3-K4 methylation|regulation of transcription-coupled nucleotide-excision repair|regulation of transcription initiation from RNA polymerase II promoter|snoRNA 3'-end processing|Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter RNA polymerase II core binding|triplex DNA binding|RNA polymerase II transcription factor binding transcription factor activity transcriptionally active chromatin|nucleus|Cdc73/Paf1 complex YHR162W YHR162W MPC2 hom Verified 3.42488315968703 0.000307531936293691 yes 40 0.000355529569729199 26 NA Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|integral to mitochondrial inner membrane YNL089C_d YNL089C YNL089C hom Dubious 3.40352566932863 0.000332610812279109 yes 41 0.000241644176664963 24 NA Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Unknown Unknown Unknown YJL216C YJL216C IMA5 hom Verified 3.37954577689958 0.000363028575275165 yes 42 0.000964959557467773 19 NA FT CARBOHYDRATE METABOLISM Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose disaccharide catabolic process oligo-1,6-glucosidase activity cellular_component YBR296C-A_p YBR296C-A YBR296C-A hom Uncharacterized 3.37412174048603 0.000370258051907803 yes 43 0.000722461590196347 36 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YLR461W YLR461W PAU4 hom Verified 3.36283890976284 0.000385726834102552 yes 44 0.000474239108983317 35 NA Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme biological_process molecular_function cellular_component YOL115W YOL115W PAP2 hom Verified 3.3490879434366 0.000405390281269236 yes 45 0.000690210018637646 49 NA FT CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION NUCLEUS Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|base-excision repair|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|histone mRNA catabolic process|nuclear polyadenylation-dependent mRNA catabolic process|nuclear polyadenylation-dependent antisense transcript catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|polyadenylation-dependent snoRNA 3'-end processing|snoRNA polyadenylation|tRNA modification 5'-deoxyribose-5-phosphate lyase activity|polynucleotide adenylyltransferase activity nucleolus|nucleus|TRAMP complex YBR174C_d YBR174C YBR174C hom Dubious 3.34880571520433 0.000405803431145954 yes 46 0.000574245246489964 39 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Unknown Unknown Unknown YCR008W YCR008W SAT4 hom Verified 3.31319534822032 0.000461182660873721 yes 47 0.000583427933826958 54 NA FT PROTEIN LOCALIZATION|CELL CYCLE|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p cellular cation homeostasis|protein phosphorylation|cellular protein localization|G1/S transition of mitotic cell cycle|protein dephosphorylation protein kinase activity cellular_component YLR426W_p YLR426W TDA5 hom Uncharacterized 3.27073516055947 0.000536341578802009 yes 48 0.00303506212831366 72 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele response to drug molecular_function mitochondrion|integral to membrane YGR241C YGR241C YAP1802 hom Verified 3.26543419107321 0.000546481995396294 yes 49 0.000938706965552975 38 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YLR394W YLR394W CST9 hom Verified 3.21206905790188 0.000658913467750293 yes 50 0.000447008342645494 16 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate synaptonemal complex assembly|protein sumoylation|synapsis|reciprocal meiotic recombination SUMO ligase activity condensed nuclear chromosome|nuclear chromosome YKL037W YKL037W AIM26 hom Verified 3.20486723680029 0.000675623960491065 yes 51 0.00252549883523854 92 NA FF|FT MITOCHONDRION Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT mitochondrion degradation molecular_function mitochondrion|integral to membrane YKL114C YKL114C APN1 hom Verified 3.20208261331165 0.000682189315696505 yes 52 0.00254049379458321 105 NA FF|FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine base-excision repair 3'-tyrosyl-DNA phosphodiesterase activity|DNA-(apurinic or apyrimidinic site) lyase activity|double-stranded DNA specific 3'-5' exodeoxyribonuclease activity|phosphoric diester hydrolase activity mitochondrion|nucleus YER002W YER002W NOP16 hom Verified 3.17307224467639 0.00075417493140483 yes 53 0.00141054896355463 28 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YML034W YML034W SRC1 hom Verified 3.1557472447257 0.000800437607056843 yes 54 0.00236344927328158 106 NA FF|FT CELL CYCLE|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance maintenance of rDNA|mitotic sister chromatid segregation molecular_function nuclear envelope YDL051W YDL051W LHP1 hom Verified 3.13628207030128 0.000855522749549556 yes 55 0.000898425923489788 25 NA FT RNA PROCESSING NUCLEUS RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen tRNA processing RNA binding|tRNA binding nucleoplasm|nucleolus|nucleus YBR023C YBR023C CHS3 hom Verified 3.12204547240359 0.000897995979822744 yes 56 0.00144514896528808 82 NA FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM SITE OF POLARIZED GROWTH Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan ascospore wall assembly|fungal-type cell wall chitin biosynthetic process|cytokinesis chitin synthase activity incipient cellular bud site|integral to membrane|chitosome|prospore membrane|cellular bud neck|mating projection tip|cytoplasm YOR112W YOR112W CEX1 hom Verified 3.12160890633467 0.000899328535633696 yes 57 0.000910930360080267 33 NA FT RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p tRNA export from nucleus tRNA binding nuclear pore|cytoplasm YOL059W YOL059W GPD2 hom Verified 3.11921901913353 0.000906655588308301 yes 58 0.00290508253279911 38 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM MITOCHONDRION NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 NADH oxidation|glycerol metabolic process glycerol-3-phosphate dehydrogenase [NAD+] activity cytosol|mitochondrion YOR113W YOR113W AZF1 hom Verified 3.10842281238453 0.000940443850685809 yes 59 0.00160084119552334 25 NA FT TRANSCRIPTION FROM RNA POL II|CELL WALL ORG/BIOGENESIS NUCLEUS Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity regulation of fungal-type cell wall organization|regulation of transcription from RNA polymerase II promoter|cellular response to carbohydrate stimulus RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YMR223W YMR223W UBP8 hom Verified 3.10027279382194 0.000966712421880236 yes 60 0.000481370413153914 25 NA FT CHROMATIN ORGANIZATION|PROTEOLYSIS NUCLEUS HISTONE ACETYLTRANSFERASE Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B histone H3-K4 methylation|histone deubiquitination|histone H3-K79 methylation ubiquitin-specific protease activity|protein complex scaffold DUBm complex|SLIK (SAGA-like) complex|SAGA complex YOR323C YOR323C PRO2 hom Verified 3.07188560892157 0.00106355611880546 no 61 0.0192273962145307 272 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS NUCLEUS Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis proline biosynthetic process glutamate-5-semialdehyde dehydrogenase activity nucleus|cytoplasm YMR189W YMR189W GCV2 hom Verified 3.05284411195052 0.00113341831604765 no 62 0.000316174206805414 29 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm glycine decarboxylation via glycine cleavage system|one-carbon metabolic process glycine dehydrogenase (decarboxylating) activity mitochondrion|glycine cleavage complex YER039C YER039C HVG1 hom Verified 3.04708495520605 0.00115536195843426 no 63 0.00130584472891602 27 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Protein of unknown function, has homology to Vrg4p biological_process molecular_function integral to membrane YDR520C_p YDR520C URC2 hom Uncharacterized 3.03276273314779 0.0012116302410361 no 64 0.00319675219148031 29 NA FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM NUCLEUS Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism uracil catabolic process sequence-specific DNA binding nucleus|cytoplasm YDR515W YDR515W SLF1 hom Verified 3.01595925028756 0.00128083842091933 no 65 0.00139225963945667 33 NA FT TRANSLATION|ION HOMEOSTASIS|ION HOMEOSTASIS RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cellular copper ion homeostasis|regulation of translation RNA binding polysome|cytoplasm YKR060W YKR060W UTP30 hom Verified 3.01231366156873 0.00129632277038572 no 66 0.00224396810518461 18 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit ribosomal small subunit biogenesis molecular_function nucleolus|nucleus|90S preribosome YLR428C_d YLR428C YLR428C hom Dubious 3.01180078028196 0.00129851487394641 no 67 0.00151301186620914 55 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Unknown Unknown Unknown YGR203W YGR203W YCH1 hom Verified 3.00533141104153 0.00132645808919941 no 68 0.00114446655566834 35 NA FT NUCLEUS Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus dephosphorylation phosphatase activity|protein tyrosine phosphatase activity|thiosulfate sulfurtransferase activity nucleus|cytoplasm YDR354W YDR354W TRP4 hom Verified 2.95727683946181 0.00155184660600426 no 69 0.090734970446343 560 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis tryptophan biosynthetic process anthranilate phosphoribosyltransferase activity nucleus|cytoplasm YHR016C YHR016C YSC84 hom Verified 2.93143729178516 0.00168698737886326 no 70 0.00203893645274201 51 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication endocytosis|actin filament organization|actin filament bundle assembly|actin cortical patch localization|actin cytoskeleton organization actin filament binding actin cortical patch YNR023W YNR023W SNF12 hom Verified 2.92057838147901 0.00174691148929913 no 71 0.0141771042514336 105 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS NUCLEUS RSC COMPLEX 73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YOL052C-A YOL052C-A DDR2 hom Verified 2.90753826652038 0.00182142885833154 no 72 0.00188885452803628 60 NA Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication response to stress molecular_function fungal-type vacuole|cytoplasm YDR459C YDR459C PFA5 hom Verified 2.88939767672623 0.00192990300773431 no 73 0.00062142755936028 15 NA FT PLASMA MEMBRANE Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain protein palmitoylation palmitoyltransferase activity integral to membrane|plasma membrane YKR072C YKR072C SIS2 hom Verified 2.86711767703711 0.00207114478800931 no 74 0.00996614897346622 149 NA FF|FT CELL CYCLE|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis regulation of mitotic cell cycle|response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|protein phosphatase inhibitor activity phosphopantothenoylcysteine decarboxylase complex|nucleus|cytoplasm YBR058C-A YBR058C-A TSC3 hom Verified 2.8615782395368 0.00210768722729793 no 75 0.0607949469629291 539 NA FF|FT KETONE METABOLISM|LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis 3-keto-sphinganine metabolic process|sphingolipid biosynthetic process enzyme activator activity SPOTS complex YBL094C_d YBL094C YBL094C hom Dubious 2.85028357987282 0.00218401326400788 no 76 0.00083293770866073 24 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Unknown Unknown Unknown YJL064W_d YJL064W YJL064W hom Dubious 2.84382962136428 0.00222874383127677 no 77 0.00181627559488494 40 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Unknown Unknown Unknown YPL180W YPL180W TCO89 hom Verified 2.83164568695358 0.002315456596226 no 78 0.00477959542290423 97 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM PLASMA MEMBRANE Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin TOR signaling cascade|fungal-type cell wall organization|response to salt stress|regulation of cell growth|glycerol metabolic process molecular_function TORC1 complex|fungal-type vacuole membrane|extrinsic to internal side of plasma membrane|extrinsic to vacuolar membrane YNL334C YNL334C SNO2 hom Verified 2.82872517110952 0.00233669061840949 no 79 0.00263832445724284 52 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin pyridoxine metabolic process|thiamine biosynthetic process molecular_function cellular_component YKL077W_p YKL077W YKL077W hom Uncharacterized 2.80296825819679 0.00253173262609571 no 80 0.00415526362025439 105 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function fungal-type vacuole YER092W YER092W IES5 hom Verified 2.80288379918424 0.00253239569966272 no 81 0.00459255944460363 99 NA FT NUCLEUS Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions biological_process molecular_function nucleus YMR070W YMR070W MOT3 hom Verified 2.80257389934871 0.00253483001642578 no 82 0.00628658033600726 120 NA FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM NUCLEUS Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion cellular hyperosmotic response|negative regulation of transcription from RNA polymerase II promoter|negative regulation of ergosterol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding nucleus YER142C YER142C MAG1 hom Verified 2.79555511561517 0.00259053333152315 no 83 0.00115122792220253 40 NA FT RESPONSE TO DNA DAMAGE NUCLEUS 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress base-excision repair, AP site formation|DNA dealkylation involved in DNA repair alkylbase DNA N-glycosylase activity nucleus YJL099W YJL099W CHS6 hom Verified 2.774478855585 0.0027645114047456 no 84 0.00512969107404113 89 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM GOLGI APPARATUS Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YIL098C YIL098C FMC1 hom Verified 2.77228704566884 0.00278319637064789 no 85 0.00377696694082969 72 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p mitochondrial proton-transporting ATP synthase complex assembly molecular_function mitochondrion|mitochondrial matrix YKR048C YKR048C NAP1 hom Verified 2.75236305800059 0.00295834430924872 no 86 0.00299629948401782 83 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress budding cell bud growth|M phase of mitotic cell cycle|nucleosome disassembly|protein import into nucleus|nucleosome assembly cyclin binding|histone binding nucleus|nuclear nucleosome|cytoplasm YDR090C_p YDR090C YDR090C hom Uncharacterized 2.75174847035361 0.00296390158300215 no 87 0.00425452957238963 54 NA FT PLASMA MEMBRANE Putative protein of unknown function biological_process molecular_function integral to membrane YIR031C YIR031C DAL7 hom Verified 2.75047943745129 0.0029754063136218 no 88 0.00355769756996256 102 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process malate synthase activity cellular_component YKR088C YKR088C TVP38 hom Verified 2.74152607251851 0.00305772535597926 no 89 0.00326563653113781 72 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern vesicle-mediated transport|mitotic spindle elongation molecular_function integral to membrane|integral to Golgi membrane|cytoplasm YIL100W_d YIL100W YIL100W hom Dubious 2.7368697617374 0.0031013422024548 no 90 0.00424935514184465 22 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Unknown Unknown Unknown YMR053C YMR053C STB2 hom Verified 2.73272898285434 0.00314059966196441 no 91 0.002363336766811 82 NA FT NUCLEUS|CHROMOSOME Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p biological_process molecular_function Sin3-type complex YDL109C_p YDL109C YDL109C hom Uncharacterized 2.73233680211547 0.00314434089896691 no 92 0.00346399613405264 6 NA FT LIPID METABOLISM Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication cellular lipid metabolic process molecular_function cellular_component YDR438W YDR438W THI74 hom Verified 2.72180399170409 0.00324633196396912 no 93 0.00395885212410678 59 NA FT MITOCHONDRION Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication biological_process molecular_function mitochondrion|integral to membrane YKL098W YKL098W MTC2 hom Verified 2.70517765014888 0.00341339347841799 no 94 0.00736812056221657 119 NA Protein of unknown function; mtc2 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YDR143C YDR143C SAN1 hom Verified 2.70356117373854 0.00343004096418555 no 95 0.00278289735992524 19 NA FT PROTEOLYSIS NUCLEUS Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition nucleus-associated proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|response to misfolded protein|response to stress ubiquitin-protein ligase activity nucleus|cytoplasm YLR422W_p YLR422W YLR422W hom Uncharacterized 2.69707125234578 0.00349761503764747 no 96 0.00514962125912463 65 NA Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein biological_process molecular_function cytoplasm YDL006W YDL006W PTC1 hom Verified 2.68885653781391 0.00358486124190353 no 97 0.0243328901189299 188 NA FF|FT RNA PROCESSING|SIGNALING|MITOCHONDRION ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation mitochondrion inheritance|inactivation of MAPK activity involved in osmosensory signaling pathway|pheromone-dependent signal transduction involved in conjugation with cellular fusion|tRNA splicing, via endonucleolytic cleavage and ligation|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YGR104C YGR104C SRB5 hom Verified 2.68885247943517 0.00358490482332866 no 98 0.00440295917433686 86 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly core mediator complex YKR067W YKR067W GPT2 hom Verified 2.67575419236322 0.00372806539789522 no 99 0.00688720216842008 91 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen phospholipid biosynthetic process glycerol-3-phosphate O-acyltransferase activity|glycerone-phosphate O-acyltransferase activity integral to membrane|lipid particle|endoplasmic reticulum|cytoplasm YCR100C_p YCR100C YCR100C hom Uncharacterized 2.67116190404633 0.00377945889369615 no 100 0.00657496055955775 64 NA Putative protein of unknown function biological_process molecular_function integral to membrane YOL111C YOL111C MDY2 hom Verified 2.66662209726612 0.00383088851003964 no 101 0.00834233524914803 99 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION NUCLEUS Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes protein insertion into ER membrane|cell morphogenesis involved in conjugation with cellular fusion|posttranslational protein targeting to membrane molecular_function ribosome|TRC complex|nucleus|cytoplasm YLR226W YLR226W BUR2 hom Verified 2.65740151288306 0.00393727975598899 no 102 0.00441331458180965 33 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION NUCLEUS Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II phosphorylation of RNA polymerase II C-terminal domain|positive regulation of histone ubiquitination|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation|mitotic sister chromatid segregation cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YOR071C YOR071C NRT1 hom Verified 2.65601809622127 0.00395346856201373 no 103 0.00268102916889794 43 NA FT PLASMA MEMBRANE High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity thiamine transport|nicotinamide riboside transport nicotinamide riboside transporter activity integral to membrane|plasma membrane YLR419W_p YLR419W YLR419W hom Uncharacterized 2.6498264542942 0.00402665633692133 no 104 0.00311436309058535 49 NA FT MITOCHONDRION Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene biological_process molecular_function mitochondrion|cytoplasm YLR438W YLR438W CAR2 hom Verified 2.64764638896633 0.00405271292049705 no 105 0.00408319674813224 57 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress ornithine metabolic process|arginine catabolic process ornithine-oxo-acid transaminase activity cytosol|nucleus|cytoplasm YDR165W YDR165W TRM82 hom Verified 2.64615239789043 0.00407065646151448 no 106 0.00553678777137731 36 NA FT RNA PROCESSING NUCLEUS Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p tRNA methylation tRNA (guanine-N7-)-methyltransferase activity tRNA methyltransferase complex|nucleus YGL007W_d YGL007W BRP1 hom Dubious 2.63924991065168 0.00415448518787573 no 107 0.0182017870053724 148 NA FF Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Unknown Unknown Unknown YNL057W_d YNL057W YNL057W hom Dubious 2.63828309654018 0.00416634936594105 no 108 0.00296003221323933 46 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR031W_p YLR031W YLR031W hom Uncharacterized 2.62334671161569 0.00435353080962806 no 109 0.0059736912289613 71 NA Putative protein of unknown function biological_process molecular_function cellular_component YJL128C YJL128C PBS2 hom Verified 2.59820116693809 0.00468567873112273 no 110 0.00999738916339372 144 NA FF|FT PROTEIN LOCALIZATION|SIGNALING|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition activation of MAPK activity involved in osmosensory signaling pathway|actin filament organization|MAPK import into nucleus involved in osmosensory signaling pathway|protein phosphorylation|hyperosmotic response|osmosensory signaling pathway|cellular response to heat MAP-kinase scaffold activity|MAP kinase kinase activity|protein kinase activity cellular bud neck|cytoplasm|cellular bud tip YDR530C YDR530C APA2 hom Verified 2.58872761681303 0.00481656294913247 no 111 0.000620522784925719 15 NA FT NUCLEOTIDE METABOLISM NUCLEUS Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication nucleoside catabolic process bis(5'-nucleosyl)-tetraphosphatase activity nucleus|cytoplasm YNR025C_d YNR025C YNR025C hom Dubious 2.58466729479466 0.00487365021495789 no 112 0.00332290874661625 70 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Unknown Unknown Unknown YMR206W_p YMR206W YMR206W hom Uncharacterized 2.58393909548166 0.00488395207929421 no 113 0.00296315829898976 59 NA Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication biological_process molecular_function cellular_component YGL058W YGL058W RAD6 hom Verified 2.58276255542362 0.00490063764983456 no 114 0.00530418553958286 53 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS|GENE SILENCING NUCLEUS|CHROMOSOME Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p protein ubiquitination involved in ubiquitin-dependent protein catabolic process|histone monoubiquitination|protein monoubiquitination|error-free translesion synthesis|error-prone translesion synthesis|transcription from RNA polymerase II promoter|mitotic cell cycle G1/S transition DNA damage checkpoint|regulation of dipeptide transport|meiotic DNA double-strand break formation|ubiquitin-dependent protein catabolic process via the N-end rule pathway|double-strand break repair via homologous recombination|protein polyubiquitination|error-free postreplication DNA repair|chromatin silencing at telomere ubiquitin-protein ligase activity nuclear chromatin|proteasome complex|nucleus|cytoplasm YNL050C_p YNL050C YNL050C hom Uncharacterized 2.5787555702675 0.00495784606406148 no 115 0.00491006980381378 49 NA Putative protein of unknown function; YNL050c is not an essential gene biological_process molecular_function cellular_component YEL014C_d YEL014C YEL014C hom Dubious 2.57479785652734 0.00501493428638759 no 116 0.00223132316500094 45 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR354C YLR354C TAL1 hom Verified 2.56723441343558 0.00512566426987476 no 117 0.0104677790622956 59 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate pentose-phosphate shunt sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glyceronetransferase activity nucleus|cytoplasm YJL098W YJL098W SAP185 hom Verified 2.5603923323909 0.00522770275453462 no 118 0.00415458022406245 63 NA FT CELL CYCLE|RNA PROCESSING MITOCHONDRION Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication tRNA wobble uridine modification|G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity mitochondrion|cytoplasm YOR009W YOR009W TIR4 hom Verified 2.55824878271136 0.00526004001479509 no 119 0.00601808520789621 70 NA FT CELL WALL ORG/BIOGENESIS Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock biological_process molecular_function fungal-type cell wall YPL091W YPL091W GLR1 hom Verified 2.54791646946312 0.00541842077947455 no 120 0.0087009449719231 127 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|protein glutathionylation glutathione-disulfide reductase activity mitochondrion|nucleus|cytoplasm YPL182C_d YPL182C YPL182C hom Dubious 2.5441499663889 0.0054772021171161 no 121 0.00522480902647967 72 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Unknown Unknown Unknown YDL113C YDL113C ATG20 hom Verified 2.54321650231281 0.00549185741567214 no 122 0.00293100814111792 35 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate mitochondrion degradation|early endosome to Golgi transport|macroautophagy|CVT pathway phosphatidylinositol-3-phosphate binding endosome|pre-autophagosomal structure|extrinsic to membrane YGR250C YGR250C YGR250C hom Verified 2.54127225507739 0.00552249382786195 no 123 0.00649518939261592 68 NA Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress biological_process RNA binding cytoplasmic stress granule|cytoplasm YNR055C YNR055C HOL1 hom Verified 2.5286774397108 0.00572465982365387 no 124 0.00424586559020223 66 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake organic alcohol transport|cation transport alcohol transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YOL049W YOL049W GSH2 hom Verified 2.52835402206598 0.0057299365721645 no 125 0.00600536858934717 61 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock glutathione biosynthetic process glutathione synthase activity intracellular YGR040W YGR040W KSS1 hom Verified 2.52493832735702 0.00578592974770511 no 126 0.00331337286797353 56 NA FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION NUCLEUS Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains pheromone-dependent signal transduction involved in conjugation with cellular fusion|signal transduction involved in filamentous growth|negative regulation of sequence-specific DNA binding transcription factor activity|protein phosphorylation|invasive growth in response to glucose limitation MAP kinase activity|protein kinase activity|transcription factor binding nucleus YGR225W YGR225W AMA1 hom Verified 2.52164225734365 0.00584042170287752 no 127 0.0046881873898358 60 NA FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION NUCLEUS UBIQUITIN LIGASE COMPLEX Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis ascospore wall assembly|positive regulation of protein catabolic process|ascospore formation|meiosis I enzyme activator activity anaphase-promoting complex YNR007C YNR007C ATG3 hom Verified 2.51419362675401 0.0059652455987381 no 128 0.00530699583977088 99 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p C-terminal protein lipidation|piecemeal microautophagy of nucleus|mitochondrion degradation|CVT pathway|autophagic vacuole assembly Atg8 ligase activity cytosol|cytoplasm YHR015W YHR015W MIP6 hom Verified 2.50853690803025 0.00606161512765216 no 129 0.00425521087122521 68 NA FT RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication mRNA export from nucleus RNA binding nuclear pore YDL168W YDL168W SFA1 hom Verified 2.49704991604343 0.00626156636397247 no 130 0.0138904435358135 78 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress furaldehyde metabolic process|formaldehyde catabolic process|amino acid catabolic process to alcohol via Ehrlich pathway S-(hydroxymethyl)glutathione dehydrogenase activity|hydroxymethylfurfural reductase (NADH) activity|alcohol dehydrogenase (NAD) activity mitochondrion|cytoplasm YIL039W YIL039W TED1 hom Verified 2.49515776026331 0.00629505704239574 no 131 0.0153637308347052 141 NA FT VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) ER to Golgi vesicle-mediated transport molecular_function endoplasmic reticulum YDR313C YDR313C PIB1 hom Verified 2.49160124467463 0.0063584358868419 no 132 0.00567711203832989 73 NA RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain protein ubiquitination ubiquitin-protein ligase activity|phosphatidylinositol-3-phosphate binding fungal-type vacuole membrane|late endosome YLR332W YLR332W MID2 hom Verified 2.48843117030999 0.00641540353969353 no 133 0.0074092268974864 131 NA FT RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PEROXISOME ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|cell morphogenesis involved in conjugation|response to acid|response to osmotic stress transmembrane signaling receptor activity integral to plasma membrane|mating projection tip YBR057C YBR057C MUM2 hom Verified 2.48766988543823 0.00642915123923498 no 134 0.0071273781469018 54 NA FT CELL CYCLE Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex premeiotic DNA replication molecular_function cytoplasm YBL022C YBL022C PIM1 hom Verified 2.48566641727035 0.00646545560953356 no 135 0.0167218010483554 148 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria chaperone-mediated protein complex assembly|misfolded or incompletely synthesized protein catabolic process ATP-dependent peptidase activity mitochondrion|mitochondrial matrix YPL055C YPL055C LGE1 hom Verified 2.47624907938349 0.00663854576128413 no 136 0.0115573680814926 148 NA FT CHROMATIN ORGANIZATION NUCLEUS Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division histone methylation|protein monoubiquitination|regulation of cell size|histone ubiquitination|premeiotic DNA replication molecular_function nucleus YOR185C YOR185C GSP2 hom Verified 2.47186825622205 0.00672045193244923 no 137 0.00942563610231396 107 NA FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM NUCLEUS GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress nucleus organization GTPase activity integral to membrane|nucleus YFR010W YFR010W UBP6 hom Verified 2.47096607934967 0.00673742997375737 no 138 0.0213061845082322 140 NA FT PROTEOLYSIS Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance protein deubiquitination ubiquitin-specific protease activity proteasome complex|proteasome regulatory particle YBR043C YBR043C QDR3 hom Verified 2.46957108430388 0.0067637569622163 no 139 0.00417657459325854 60 NA FT PLASMA MEMBRANE Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin drug transmembrane transport|polyamine homeostasis drug transmembrane transporter activity|polyamine transmembrane transporter activity integral to membrane|plasma membrane YDR290W_d YDR290W YDR290W hom Dubious 2.46132095847333 0.00692132345702684 no 140 0.0173116100070679 136 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Unknown Unknown Unknown YIR020W-A_d YIR020W-A YIR020W-A hom Dubious 2.45067042976731 0.00712952210000945 no 141 0.00922951445515324 75 NA Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YBR285W_p YBR285W YBR285W hom Uncharacterized 2.44688617754288 0.00720481685227828 no 142 0.0122526711761684 105 NA Putative protein of unknown function; YBR285W is not an essential gene biological_process molecular_function cellular_component YMR056C YMR056C AAC1 hom Verified 2.43262407976948 0.00749492729496188 no 143 0.0056650852882346 89 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress heme biosynthetic process|mitochondrial transport|heme transport|aerobic respiration ATP:ADP antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YDR253C YDR253C MET32 hom Verified 2.42975185160102 0.00755458176391891 no 144 0.0114302941711944 136 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM NUCLEUS Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication regulation of mitotic cell cycle|regulation of sulfur amino acid metabolic process|regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|RNA polymerase II activating transcription factor binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YDL173W YDL173W PAR32 hom Verified 2.42487142688124 0.00765690460796058 no 145 0.0156232681401165 126 NA Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene biological_process molecular_function cytoplasm YPL052W YPL052W OAZ1 hom Verified 2.42374959473935 0.00768059665597153 no 146 0.00556643679995117 63 NA FT TRANSLATION|PROTEOLYSIS Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Unknown|regulation of proteasomal protein catabolic process ornithine decarboxylase inhibitor activity cytoplasm YFR012W YFR012W DCV1 hom Verified 2.41405758135322 0.00788798448957919 no 147 0.00409458233805038 49 NA Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication biological_process molecular_function integral to membrane YFR036W YFR036W CDC26 hom Verified 2.41221242888763 0.00792802008983843 no 148 0.0180812669432891 222 NA FF|FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YIL072W YIL072W HOP1 hom Verified 2.40660993953718 0.00805067844970735 no 149 0.0180426103495937 130 NA FT CELL CYCLE NUCLEUS|CHROMOSOME Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments synaptonemal complex assembly|meiotic recombination checkpoint|synapsis four-way junction DNA binding condensed nuclear chromosome|lateral element YMR179W YMR179W SPT21 hom Verified 2.40441619564551 0.00809915987641897 no 150 0.0103664766592204 119 NA FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING NUCLEUS Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress chromatin silencing at rDNA|regulation of transcription from RNA polymerase II promoter|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette molecular_function nucleus YOR367W YOR367W SCP1 hom Verified 2.4030272194947 0.00812998854329125 no 151 0.012938957676717 106 NA FT CYTOSKELETON ORGANIZATION CYTOSKELETON Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin actin filament organization|chronological cell aging actin filament binding|protein binding, bridging actin cortical patch YNL279W YNL279W PRM1 hom Verified 2.39707972484184 0.00826316373485255 no 152 0.00827025707963149 93 NA FT MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p plasma membrane fusion involved in cytogamy|response to pheromone molecular_function integral to membrane|mating projection tip YHR184W YHR184W SSP1 hom Verified 2.39074909460124 0.00840701952498252 no 153 0.00798529835786704 98 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis ascospore wall assembly|protein complex assembly molecular_function prospore membrane YEL018W YEL018W EAF5 hom Verified 2.38121457593692 0.00862782864525387 no 154 0.0120776362977428 87 NA FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS HISTONE ACETYLTRANSFERASE Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex DNA repair molecular_function nucleus|NuA4 histone acetyltransferase complex YDL085C-A_p YDL085C-A YDL085C-A hom Uncharacterized 2.37432887754998 0.00879044167217601 no 155 0.00918378468605748 71 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YLL013C YLL013C PUF3 hom Verified 2.36984374428617 0.00889780214732267 no 156 0.00728828610175149 87 NA FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|RNA LOCALIZATION MITOCHONDRION Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|nuclear-transcribed mRNA catabolic process|intracellular mRNA localization|mitochondrion organization|aerobic respiration|mitochondrion localization mRNA binding external side of mitochondrial outer membrane|cytoplasm YOL107W_p YOL107W YOL107W hom Uncharacterized 2.35700483756349 0.00921150342885544 no 157 0.00681789907381311 91 NA FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein biological_process molecular_function COPI-coated vesicle|Golgi apparatus YDL048C YDL048C STP4 hom Verified 2.3532124695469 0.00930599793679274 no 158 0.0132824143664937 82 NA FT NUCLEUS|MITOCHONDRION Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress biological_process sequence-specific DNA binding mitochondrion|nucleus|cytoplasm YNR018W YNR018W RCF2 hom Verified 2.3507547387353 0.00936768923821894 no 159 0.00670339458201645 82 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|mitochondrial respiratory chain complex IV|integral to mitochondrial inner membrane YDR481C YDR481C PHO8 hom Verified 2.34870598266209 0.00941938800707126 no 160 0.0101989082208979 93 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN nicotinamide nucleotide metabolic process|protein dephosphorylation nucleotide phosphatase activity|alkaline phosphatase activity fungal-type vacuole membrane YIL092W_p YIL092W YIL092W hom Uncharacterized 2.34709332388393 0.00946025754843862 no 161 0.00803708189801483 80 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus biological_process molecular_function nucleus|cytoplasm YPL199C_p YPL199C YPL199C hom Uncharacterized 2.33901666301424 0.00966728556433599 no 162 0.00996405114653958 93 NA Putative protein of unknown function, predicted to be palmitoylated biological_process molecular_function cytoplasm YPR050C_d YPR050C YPR050C hom Dubious 2.33518837096398 0.00976679118652282 no 163 0.0220527854057431 148 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Unknown Unknown Unknown YCL036W YCL036W GFD2 hom Verified 2.32868790387434 0.00993780269501723 no 164 0.00970859658718812 106 NA Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL244C YPL244C HUT1 hom Verified 2.3275091496662 0.00996909129306194 no 165 0.00969712323635256 96 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 UDP-galactose transport|UDP-glucose transport UDP-galactose transmembrane transporter activity integral to membrane YLL060C YLL060C GTT2 hom Verified 2.31954213134726 0.010182829725253 no 166 0.00976521887358318 105 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress glutathione metabolic process glutathione transferase activity mitochondrion YMR062C YMR062C ARG7 hom Verified 2.31871326572421 0.0102052943760443 no 167 0.0181005705318252 136 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS MITOCHONDRION Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine fungal-type cell wall organization|ornithine biosynthetic process|arginine biosynthetic process acetyl-CoA:L-glutamate N-acetyltransferase activity|glutamate N-acetyltransferase activity mitochondrion|mitochondrial matrix YGL090W YGL090W LIF1 hom Verified 2.31654747687611 0.0102641976686229 no 168 0.0109929532403339 124 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein double-strand break repair via nonhomologous end joining chromatin binding DNA ligase IV complex|nuclear chromatin|nucleus YKL110C YKL110C KTI12 hom Verified 2.3129670229266 0.0103622261541022 no 169 0.0140707561384486 133 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p regulation of transcription from RNA polymerase II promoter|cell growth|tRNA wobble uridine modification chromatin binding nucleus|cytoplasm YGR130C YGR130C YGR130C hom Verified 2.30609388966418 0.0105526933335371 no 170 0.0176085297367114 132 NA Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) biological_process molecular_function eisosome|membrane raft|cytoplasm YPL056C_p YPL056C LCL1 hom Uncharacterized 2.30608577799719 0.0105529199128338 no 171 0.0157038389426013 110 NA FT PLASMA MEMBRANE Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan biological_process molecular_function cellular_component YNL239W YNL239W LAP3 hom Verified 2.30358589952238 0.0106229500631037 no 172 0.00796806205283374 97 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS MITOCHONDRION Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH response to antibiotic|negative regulation of transcription from RNA polymerase II promoter|homocysteine catabolic process double-stranded DNA binding|cysteine-type peptidase activity|mRNA binding|single-stranded DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding mitochondrion|cytoplasm YJL218W_p YJL218W YJL218W hom Uncharacterized 2.29363782180855 0.0109056555021274 no 173 0.0109816909498402 95 NA Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene biological_process acetyltransferase activity cellular_component YOR049C YOR049C RSB1 hom Verified 2.2912363457065 0.0109748739536524 no 174 0.0100121278386036 81 NA FT MEMBRANE ORGANIZATION ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane phospholipid translocation|fatty acid transport phospholipid-translocating ATPase activity integral to membrane|endoplasmic reticulum|plasma membrane YBR274W YBR274W CHK1 hom Verified 2.28882942325718 0.0110446326020061 no 175 0.00719696017703466 51 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase protein phosphorylation|replication fork protection|DNA damage checkpoint protein serine/threonine kinase activity nucleus YBR246W YBR246W RRT2 hom Verified 2.28447295800265 0.0111718750445589 no 176 0.0176929519290393 176 NA FT VESICLE-MEDIATED TRANSPORT Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription peptidyl-diphthamide biosynthetic process from peptidyl-histidine|endocytic recycling molecular_function endosome|cytoplasm YCR082W YCR082W AHC2 hom Verified 2.27885763769936 0.0113377646661109 no 177 0.0180284678898364 137 NA FT CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex histone acetylation histone acetyltransferase activity Ada2/Gcn5/Ada3 transcription activator complex|nucleus|cytoplasm YOR313C YOR313C SPS4 hom Verified 2.27053247726942 0.0115876475402229 no 178 0.0106187505332372 98 NA FT CELL CYCLE Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage ascospore formation molecular_function cellular_component YER113C YER113C TMN3 hom Verified 2.26811223880738 0.0116611833025091 no 179 0.00862666856044263 101 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation cellular copper ion homeostasis|vacuolar transport|pseudohyphal growth|invasive growth in response to glucose limitation molecular_function COPI-coated vesicle|integral to membrane|Golgi apparatus YKL126W YKL126W YPK1 hom Verified 2.26638370276239 0.011713950271846 no 180 0.131528678945306 618 NA FF|FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication eisosome assembly|protein autophosphorylation|negative regulation of phospholipid translocation|protein phosphorylation protein serine/threonine kinase activity cytosol|plasma membrane|cellular bud neck YNL298W YNL298W CLA4 hom Verified 2.26304344027718 0.0118165057884181 no 181 0.0350467197136059 261 NA FF|FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p negative regulation of gene expression|vacuole inheritance|protein phosphorylation|budding cell apical bud growth|Rho protein signal transduction|regulation of exit from mitosis|establishment of cell polarity|sterol import|response to pheromone|cytokinesis protein serine/threonine kinase activity|protein kinase activity nucleus|cellular bud|fungal-type vacuole YDR176W YDR176W NGG1 hom Verified 2.26259129030851 0.0118304477651961 no 182 0.0260669734778187 210 NA FF|FT CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex histone acetylation|chromatin modification histone acetyltransferase activity|transcription cofactor activity SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YBR025C YBR025C OLA1 hom Verified 2.26257375863214 0.0118309886391305 no 183 0.0123813950576574 98 NA P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress biological_process ATPase activity cytoplasm YDR466W YDR466W PKH3 hom Verified 2.25639288277847 0.012023018488915 no 184 0.0384769531422116 196 NA FF|FT SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant MAPK cascade involved in cell wall biogenesis|protein phosphorylation protein kinase activity cellular_component YMR082C_d YMR082C YMR082C hom Dubious 2.25516620766127 0.0120614490758121 no 185 0.0167766226263322 92 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR227W YGR227W DIE2 hom Verified 2.25282227064571 0.0121351786993229 no 186 0.0116563922547884 132 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 dolichol-linked oligosaccharide biosynthetic process|protein N-linked glycosylation dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity integral to membrane|endoplasmic reticulum membrane YJL047C YJL047C RTT101 hom Verified 2.25259815687508 0.0121422487217302 no 187 0.02005044365377 170 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p replication fork processing|negative regulation of transposition, RNA-mediated|regulation of mitosis|nonfunctional rRNA decay|ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity Cul8-RING ubiquitin ligase complex|nucleus|cytoplasm YDR401W_d YDR401W YDR401W hom Dubious 2.24934659481738 0.0122452267587781 no 188 0.0144093072407191 58 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR453C YLR453C RIF2 hom Verified 2.24516788402791 0.0123786784992465 no 189 0.0097085104969503 135 NA FT NUCLEUS|CHROMOSOME Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation telomere capping|telomere maintenance via telomerase telomeric DNA binding nuclear telomere cap complex YHR021W-A_p YHR021W-A ECM12 hom Uncharacterized 2.23872535392367 0.012586895898814 no 190 0.00968561758367774 87 NA FT CELL WALL ORG/BIOGENESIS Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity fungal-type cell wall organization molecular_function cellular_component YDL190C YDL190C UFD2 hom Verified 2.23862942388052 0.0125900190498864 no 191 0.015227976232785 117 NA FT PROTEOLYSIS NUCLEUS Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 ER-associated protein catabolic process|protein ubiquitination|ubiquitin-dependent protein catabolic process|response to stress ubiquitin-ubiquitin ligase activity nucleus|cytoplasm YFL053W YFL053W DAK2 hom Verified 2.23668740390256 0.012653388915582 no 192 0.00843861203939321 96 NA FT CARBOHYDRATE METABOLISM Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation response to stress|glycerol catabolic process glycerone kinase activity cellular_component YPR009W YPR009W SUT2 hom Verified 2.23600131525373 0.0126758425017811 no 193 0.0167247202681297 93 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|sequence-specific DNA binding nucleus YPR015C_p YPR015C YPR015C hom Uncharacterized 2.23588969483582 0.0126794987564448 no 194 0.0158509935594925 91 NA Putative protein of unknown function; overexpression causes a cell cycle delay or arrest biological_process sequence-specific DNA binding cellular_component YKL101W YKL101W HSL1 hom Verified 2.23134894988563 0.0128290117972792 no 195 0.0149329690342386 149 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p G2/M transition of mitotic cell cycle|regulation of cell cycle|cell shape checkpoint|protein autophosphorylation|protein phosphorylation|septin checkpoint protein kinase activity cellular bud neck|septin ring YKL033W-A_p YKL033W-A YKL033W-A hom Uncharacterized 2.2212820840242 0.013165930776842 no 196 0.0235903362810752 167 NA Putative protein of unknown function; similar to uncharacterized proteins from other fungi biological_process molecular_function cellular_component YDL052C YDL052C SLC1 hom Verified 2.20414676828791 0.0137570119907062 no 197 0.0213062837518983 128 NA FT LIPID METABOLISM 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes glycerophospholipid biosynthetic process 1-acylglycerol-3-phosphate O-acyltransferase activity lipid particle YLR406C YLR406C RPL31B hom Verified 2.20187556148721 0.0138370498527413 no 198 0.0283804442509743 139 NA FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication cytoplasmic translation|regulation of translational fidelity structural constituent of ribosome cytosolic large ribosomal subunit YML005W YML005W TRM12 hom Verified 2.19190161024641 0.0141933058959424 no 199 0.0174272963572958 100 NA FT RNA PROCESSING S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA wybutosine biosynthetic process|tRNA methylation S-adenosylmethionine-dependent methyltransferase activity|transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm YDL144C YDL144C YDL144C hom Verified 2.19024453702314 0.014253253415256 no 200 0.0174935027174679 124 NA FT OXIDATION-REDUCTION PROCESS NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YOR193W YOR193W PEX27 hom Verified 2.17619999506147 0.0147701546479861 no 201 0.0130304951270449 95 NA FT PEROXISOME ORGANIZATION Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p peroxisome organization molecular_function peroxisomal membrane YNL214W YNL214W PEX17 hom Verified 2.17325087686076 0.0148807202410265 no 202 0.0190327008671425 124 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis protein import into peroxisome matrix, docking protein binding peroxisomal membrane YOR321W YOR321W PMT3 hom Verified 2.16695359618644 0.0151191962985445 no 203 0.0126479571814862 109 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication protein O-linked glycosylation|protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YBR271W YBR271W EFM2 hom Verified 2.16435982414116 0.0152183723423649 no 204 0.0211581540644862 119 NA S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis peptidyl-lysine methylation S-adenosylmethionine-dependent methyltransferase activity|protein-lysine N-methyltransferase activity cytoplasm YOR018W YOR018W ROD1 hom Verified 2.15892474515361 0.0154280028799594 no 205 0.00945176297451661 84 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION PLASMA MEMBRANE Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication response to drug|ubiquitin-dependent endocytosis ubiquitin protein ligase binding plasma membrane YNL199C YNL199C GCR2 hom Verified 2.15807648836475 0.0154609427087533 no 206 0.0216742500858738 157 NA FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|NUCLEUS Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity nuclear envelope|nucleus YBR183W YBR183W YPC1 hom Verified 2.15798844109314 0.0154643652497485 no 207 0.0186079480251109 116 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication ceramide catabolic process|ceramide biosynthetic process phytoceramidase activity|sphingosine N-acyltransferase activity integral to membrane|endoplasmic reticulum YGL244W YGL244W RTF1 hom Verified 2.15703487018365 0.0155014737894224 no 208 0.0499364433348963 243 NA FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|GENE SILENCING NUCLEUS|CHROMOSOME RNA POL II, HOLOENZYME Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay mRNA 3'-end processing|snoRNA transcription from an RNA polymerase II promoter|regulation of chromatin silencing at telomere|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|regulation of histone H3-K4 methylation|regulation of transcription-coupled nucleotide-excision repair|negative regulation of transcription from RNA polymerase II promoter|recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex|global genome nucleotide-excision repair|snoRNA 3'-end processing|Unknown|transcription elongation from RNA polymerase II promoter RNA binding|RNA polymerase II transcription factor binding transcription factor activity nucleus|transcriptionally active chromatin|Cdc73/Paf1 complex YDL066W YDL066W IDP1 hom Verified 2.15080014049919 0.0157459890088275 no 209 0.0457071620939763 286 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes isocitrate metabolic process|glutamate biosynthetic process isocitrate dehydrogenase (NADP+) activity mitochondrial nucleoid|mitochondrion YBR279W YBR279W PAF1 hom Verified 2.14812514105995 0.0158519077464397 no 210 0.0357609887009273 234 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|GENE SILENCING NUCLEUS|CHROMOSOME RNA POL II, HOLOENZYME Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 negative regulation of DNA recombination|mRNA 3'-end processing|snoRNA transcription from an RNA polymerase II promoter|regulation of transcription by chromatin organization|transcription from RNA polymerase I promoter|regulation of transcription-coupled nucleotide-excision repair|rRNA processing|transcription elongation from RNA polymerase I promoter|regulation of chromatin silencing at telomere|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of histone H3-K4 methylation|negative regulation of transcription from RNA polymerase II promoter|global genome nucleotide-excision repair|chromatin silencing at rDNA|Unknown|snoRNA 3'-end processing|transcription elongation from RNA polymerase II promoter|regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity|chromatin binding|TFIIF-class binding transcription factor activity|RNA polymerase II core binding|RNA polymerase II transcription factor binding transcription factor activity transcriptionally active chromatin|nucleus|Cdc73/Paf1 complex YNR071C_p YNR071C YNR071C hom Uncharacterized 2.14637596143066 0.0159214977991246 no 211 0.0123611517522319 106 NA FT CARBOHYDRATE METABOLISM Putative aldose 1-epimerase biological_process molecular_function cellular_component YEL047C YEL047C FRD1 hom Verified 2.14583525555981 0.0159430623827822 no 212 0.0146969994898138 121 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity cytosol|mitochondrion|ribosome|plasma membrane YJR107W_p YJR107W YJR107W hom Uncharacterized 2.14560212086278 0.0159523680492835 no 213 0.0136531892184719 88 NA FT LIPID METABOLISM Putative lipase biological_process lipase activity cellular_component YBL056W YBL056W PTC3 hom Verified 2.14504955803497 0.0159744424186067 no 214 0.0130712739319211 132 NA FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION NUCLEUS Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of cyclin-dependent protein kinase activity|protein dephosphorylation protein serine/threonine phosphatase activity nucleus|cytoplasm YPL049C YPL049C DIG1 hom Verified 2.14309483843431 0.0160527419234552 no 215 0.0241585054571206 199 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown DNA binding|transcription factor binding nucleus YER076C_p YER076C YER076C hom Uncharacterized 2.14155560254635 0.0161146298146416 no 216 0.0143072181584846 97 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization biological_process molecular_function mitochondrion YPL037C YPL037C EGD1 hom Verified 2.14103303681278 0.0161356869949162 no 217 0.0216576529339062 144 NA FT PROTEIN LOCALIZATION NUCLEUS Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b 'de novo' cotranslational protein folding unfolded protein binding nascent polypeptide-associated complex YOR352W YOR352W TFB6 hom Verified 2.13947955627274 0.0161984249445356 no 218 0.0145606629985588 118 NA FT NUCLEUS Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|holo TFIIH complex|cytoplasm YHR049C-A_d YHR049C-A YHR049C-A hom Dubious 2.13759940659859 0.0162746349761759 no 219 0.0122292182218619 92 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR282C_d YLR282C YLR282C hom Dubious 2.11384168981909 0.0172643954458371 no 220 0.0123355459038492 103 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YBR203W YBR203W COS111 hom Verified 2.106163785559 0.0175950638508195 no 221 0.0299922062379678 152 NA FT SIGNALING MITOCHONDRION Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies signal transduction molecular_function mitochondrion YLR384C YLR384C IKI3 hom Verified 2.10597564317706 0.0176032340695253 no 222 0.0158064166943746 151 NA FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification molecular_function Elongator holoenzyme complex|nucleus|cytoplasm YKL081W YKL081W TEF4 hom Verified 2.10449333765942 0.017667717583126 no 223 0.0212834666388239 121 NA FT TRANSLATION MITOCHONDRION RIBOSOME Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex translational elongation translation elongation factor activity mitochondrion|ribosome|eukaryotic translation elongation factor 1 complex YDR409W YDR409W SIZ1 hom Verified 2.10207284999475 0.0177734473774434 no 224 0.0185477625037845 124 NA FT CHROMOSOME SEGREGATION NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring protein sumoylation|chromosome segregation SUMO ligase activity nuclear chromatin|septin ring YLR085C YLR085C ARP6 hom Verified 2.10171992266745 0.0177889086667991 no 225 0.0385504805179305 285 NA FF|FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A chromatin remodeling|histone exchange nucleosome binding Swr1 complex|cytoplasm YBR259W YBR259W YBR259W hom Verified 2.09767080098573 0.0179671179765711 no 226 0.018653975595096 111 NA Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress biological_process molecular_function cellular_component YLR412C-A_p YLR412C-A YLR412C-A hom Uncharacterized 2.09436913082935 0.0181135552086601 no 227 0.0171411258974442 107 NA Putative protein of unknown function biological_process molecular_function cellular_component YDL019C YDL019C OSH2 hom Verified 2.09425510201587 0.0181186307897272 no 228 0.030492269942184 139 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability endocytosis|maintenance of cell polarity|sterol transport|exocytosis lipid binding|oxysterol binding|sterol transporter activity|1-phosphatidylinositol binding endoplasmic reticulum|plasma membrane|cortical endoplasmic reticulum|cellular bud neck YLR084C YLR084C RAX2 hom Verified 2.09374286978129 0.018141445915628 no 229 0.0255385330778123 92 NA FT CELL CYCLE|CELL DIVISION MITOCHONDRION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p cellular bud site selection molecular_function mitochondrion|cellular bud scar|cellular bud neck|membrane YNL233W YNL233W BNI4 hom Verified 2.09087362037611 0.0182696971966516 no 230 0.031156938745852 173 NA FT PROTEIN LOCALIZATION|CELL DIVISION|CARBOHYDRATE METABOLISM SITE OF POLARIZED GROWTH|CYTOSKELETON Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p asymmetric protein localization|barrier septum assembly|chitin biosynthetic process protein binding incipient cellular bud site|cellular bud neck septin collar|cellular bud neck|septin ring YJL182C_d YJL182C YJL182C hom Dubious 2.08160328431083 0.0186893605884383 no 231 0.0177071950514522 128 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Unknown Unknown Unknown YGR260W YGR260W TNA1 hom Verified 2.07836822958393 0.0188377282906056 no 232 0.026093395244372 144 NA FT MITOCHONDRION|PLASMA MEMBRANE High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) nicotinamide mononucleotide transport nicotinamide mononucleotide transmembrane transporter activity mitochondrion|integral to membrane|integral to plasma membrane YBL048W_d YBL048W RRT1 hom Dubious 2.07612559491275 0.0189411682601894 no 233 0.0284141231502037 180 NA Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YHR079C-A YHR079C-A SAE3 hom Verified 2.07600546107938 0.0189467229569326 no 234 0.0250162306730266 116 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p double-strand break repair|reciprocal meiotic recombination|meiotic DNA recombinase assembly molecular_function condensed nuclear chromosome YOL055C YOL055C THI20 hom Verified 2.07031620470596 0.0192113715345226 no 235 0.0116945144927209 77 NA Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p thiamine biosynthetic process|thiamine catabolic process phosphomethylpyrimidine kinase activity|thiaminase activity|hydroxymethylpyrimidine kinase activity cytosol YLR014C YLR014C PPR1 hom Verified 2.0665986033096 0.0193859962681239 no 236 0.0347759225256911 168 NA FT TRANSCRIPTION FROM RNA POL II|NUCLEOTIDE METABOLISM NUCLEUS Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity|sequence-specific DNA binding|zinc ion binding nucleus YLR273C YLR273C PIG1 hom Verified 2.05939036903827 0.0197284288136868 no 237 0.0087460404638722 90 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase regulation of glycogen biosynthetic process protein phosphatase type 1 regulator activity protein phosphatase type 1 complex YJL132W_p YJL132W YJL132W hom Uncharacterized 2.0592008105668 0.0197375027833872 no 238 0.0162491734291517 111 NA Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene biological_process molecular_function membrane YER034W YER034W YER034W hom Verified 2.05764478001726 0.019812122362637 no 239 0.0242741598930067 129 NA FT NUCLEUS Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YPR063C_p YPR063C YPR063C hom Uncharacterized 2.04793846919262 0.0202830144291121 no 240 0.0260816551744638 165 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER-localized protein of unknown function biological_process molecular_function endoplasmic reticulum YOL002C YOL002C IZH2 hom Verified 2.0455105343624 0.0204022758583567 no 241 0.0183885643161601 165 NA FT KETONE METABOLISM|LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc response to zinc ion|cellular zinc ion homeostasis|response to toxin|cellular lipid metabolic process protein binding|metal ion binding integral to membrane|plasma membrane|membrane YMR209C_p YMR209C YMR209C hom Uncharacterized 2.04386431147722 0.0204834768353878 no 242 0.0204431284172171 158 NA Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cellular_component YDR457W YDR457W TOM1 hom Verified 2.04211783133855 0.0205699222615444 no 243 0.0138436140825906 142 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEOLYSIS|RNA LOCALIZATION NUCLEUS E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mitosis|nucleocytoplasmic transport|nucleus organization|cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of cell size|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|nucleolus|nucleus YMR087W YMR087W YMR087W hom Verified 2.04067497362498 0.0206415722544183 no 244 0.0198292919881293 159 NA FT RNA PROCESSING Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain tRNA splicing, via endonucleolytic cleavage and ligation phosphatase activity cellular_component YDR192C YDR192C NUP42 hom Verified 2.03533928188513 0.0209083731036823 no 245 0.0176541853087046 109 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|mRNA export from nucleus in response to heat stress|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YGR069W_d YGR069W YGR069W hom Dubious 2.03497563075043 0.0209266625380292 no 246 0.0290202461796424 153 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR136C YHR136C SPL2 hom Verified 2.03397053261638 0.0209772832945662 no 247 0.0234103090582635 143 NA Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm response to temperature stimulus enzyme inhibitor activity cytoplasm YAL062W YAL062W GDH3 hom Verified 2.01774266465701 0.0218090344755566 no 248 0.0134892650698633 93 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS NUCLEUS|MITOCHONDRION NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication glutamate biosynthetic process|ammonia assimilation cycle glutamate dehydrogenase (NADP+) activity mitochondrion|nucleus YCR032W YCR032W BPH1 hom Verified 2.01346772202437 0.0220327236811079 no 249 0.022481556747906 175 NA FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS MITOCHONDRION Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting intracellular protein transport|fungal-type cell wall organization|response to pH molecular_function cytosol|mitochondrion|extrinsic to membrane YML121W YML121W GTR1 hom Verified 2.01311619707127 0.0220512033273706 no 250 0.0434146432192652 246 NA FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|GENE SILENCING NUCLEUS Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB phosphate ion transport|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|chromatin silencing at telomere GDP binding|GTP binding fungal-type vacuole membrane|EGO complex|nucleus|late endosome membrane|cytoplasm YBR215W YBR215W HPC2 hom Verified 2.0003396151683 0.0227318020231781 no 251 0.0224786320171075 139 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes DNA replication-independent nucleosome assembly|regulation of transcription involved in G1/S phase of mitotic cell cycle|transcription elongation from RNA polymerase II promoter nucleosome binding|DNA binding HIR complex|nucleus YPR139C YPR139C LOA1 hom Verified 1.99992921893248 0.0227539537569276 no 252 0.0424300061739636 213 NA FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT ENDOPLASMIC RETICULUM Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA lipid particle organization|cellular triglyceride homeostasis|protein targeting to vacuole lysophosphatidic acid acyltransferase activity endoplasmic reticulum|lipid particle|cytoplasm YER180C YER180C ISC10 hom Verified 1.99161752124941 0.02320651972679 no 253 0.0226772193919652 142 NA FT CELL CYCLE Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells sporulation resulting in formation of a cellular spore molecular_function cellular_component YDR335W YDR335W MSN5 hom Verified 1.99155737575992 0.0232098220216427 no 254 0.0313348547825603 199 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION NUCLEUS Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm tRNA re-export from nucleus|protein export from nucleus protein binding|importin-alpha export receptor activity integral to membrane|nucleus|cytoplasm YDL095W YDL095W PMT1 hom Verified 1.98785624299299 0.0234137956610723 no 255 0.0407399531528496 168 NA FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen ER-associated misfolded protein catabolic process|Unknown|protein exit from endoplasmic reticulum|protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YHR130C_d YHR130C YHR130C hom Dubious 1.98744474593531 0.0234365666227455 no 256 0.0263670956503224 126 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR086W-A_p YHR086W-A YHR086W-A hom Uncharacterized 1.97666488856389 0.0240397610696327 no 257 0.0214221857024258 133 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGL057C YGL057C GEP7 hom Verified 1.97552256894841 0.0241044380040106 no 258 0.0289044310962153 136 NA FT MITOCHONDRION Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YHR194W YHR194W MDM31 hom Verified 1.96286572562493 0.0248308891260428 no 259 0.0320965828908872 195 NA FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS NUCLEUS|MITOCHONDRION Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 mitochondrion inheritance|cellular ion homeostasis|mitochondrion organization|Unknown molecular_function mitochondrial inner membrane|nucleus YPR046W YPR046W MCM16 hom Verified 1.96090041588324 0.0249453204068133 no 260 0.0472225426648685 255 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YDR504C YDR504C SPG3 hom Verified 1.95878393747171 0.0250690477350055 no 261 0.0146828120861089 109 NA Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources biological_process molecular_function cellular_component YMR068W YMR068W AVO2 hom Verified 1.95582644042896 0.0252428015243107 no 262 0.0265158984759714 136 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity molecular_function TORC2 complex|cytoplasm YGL117W_p YGL117W YGL117W hom Uncharacterized 1.95208656665386 0.0254639639169564 no 263 0.0590266408974846 355 NA FF Putative protein of unknown function biological_process molecular_function cellular_component YPL234C YPL234C VMA11 hom Verified 1.94729180111442 0.0257498805520161 no 264 0.0295863683385868 158 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane|fungal-type vacuole YBR051W_d YBR051W YBR051W hom Dubious 1.94420272812274 0.0259355044713525 no 265 0.0256675635694988 157 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Unknown Unknown Unknown YKL213C YKL213C DOA1 hom Verified 1.94416377687588 0.0259378521992091 no 266 0.0241077504887818 164 NA FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress double-strand break repair via nonhomologous end joining|ubiquitin-dependent protein catabolic process ubiquitin binding nucleus|cytoplasm YPR158W YPR158W CUR1 hom Verified 1.94355534906223 0.025974547356219 no 267 0.0319725823383798 224 NA FT NUCLEUS Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication cellular response to stress|protein localization to nucleus chaperone binding nucleus YDL077C YDL077C VAM6 hom Verified 1.94313922494274 0.0259996694007238 no 268 0.0613594263926321 320 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle-mediated transport|vacuole organization|vacuole fusion, non-autophagic Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding fungal-type vacuole membrane|HOPS complex|fungal-type vacuole YFL020C YFL020C PAU5 hom Verified 1.93610961892272 0.0264271383469939 no 269 0.0171639194015191 126 NA Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme biological_process molecular_function integral to membrane|cytoplasm YBL071W-A YBL071W-A KTI11 hom Verified 1.93101295677448 0.0267407250629366 no 270 0.048630096634034 258 NA FT RNA PROCESSING NUCLEUS Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs peptidyl-diphthamide biosynthetic process from peptidyl-histidine|tRNA wobble uridine modification electron carrier activity|iron ion binding|zinc ion binding nucleus|cytoplasm YMR196W_p YMR196W YMR196W hom Uncharacterized 1.92364819534797 0.0271993478064406 no 271 0.0388911889157345 239 NA FT CARBOHYDRATE METABOLISM Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene biological_process molecular_function cytoplasm YML051W YML051W GAL80 hom Verified 1.91905725295521 0.0274885442756967 no 272 0.0323204403605133 129 NA FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM NUCLEUS Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding negative regulation of kinase activity|galactose metabolic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity RNA polymerase II activating transcription factor binding|kinase inhibitor activity nucleus|cytoplasm YMR123W YMR123W PKR1 hom Verified 1.9182118062794 0.0275420799058089 no 273 0.0718447844569749 338 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress vacuolar proton-transporting V-type ATPase complex assembly molecular_function integral to endoplasmic reticulum membrane YNL286W YNL286W CUS2 hom Verified 1.91100128287625 0.0280022080168612 no 274 0.029605903995479 191 NA FT RNA PROCESSING NUCLEUS Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) mRNA splicing, via spliceosome RNA binding U2 snRNP YHR177W_p YHR177W YHR177W hom Uncharacterized 1.90740246573548 0.028234245168264 no 275 0.0289050175566693 180 NA Putative protein of unknown function; overexpression causes a cell cycle delay or arrest biological_process molecular_function cellular_component YCR015C_p YCR015C YCR015C hom Uncharacterized 1.90713552640903 0.0282515199088665 no 276 0.0206786710982637 152 NA Putative protein of unknown function; YCR015C is not an essential gene biological_process molecular_function cellular_component YER049W YER049W TPA1 hom Verified 1.90700146107927 0.0282601991453795 no 277 0.0163948970139861 95 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS NUCLEUS Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|translational termination poly(A) RNA binding nucleus YMR313C YMR313C TGL3 hom Verified 1.90390676934734 0.0284611636708315 no 278 0.0249740653176432 155 NA FT LIPID METABOLISM|CELL DIVISION Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation triglyceride catabolic process|cell budding|cellular lipid metabolic process triglyceride lipase activity|lysophosphatidylethanolamine acyltransferase activity lipid particle YLR395C YLR395C COX8 hom Verified 1.90362078305527 0.0284797950478847 no 279 0.0396946583143097 184 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain mitochondrial electron transport, cytochrome c to oxygen cytochrome-c oxidase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex IV YDR494W YDR494W RSM28 hom Verified 1.9019327295249 0.0285899748364173 no 280 0.0317389652144553 202 NA FT TRANSLATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation cytoplasmic translation|translational initiation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YPL270W YPL270W MDL2 hom Verified 1.90160362788525 0.0286114966501717 no 281 0.0368096739041791 206 NA FT NUCLEOTIDE METABOLISM MITOCHONDRION Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis oligopeptide transport|oligopeptide export from mitochondrion oligopeptide-transporting ATPase activity mitochondrion|integral to membrane|mitochondrial inner membrane YOR355W YOR355W GDS1 hom Verified 1.89773977398338 0.0288651851633675 no 282 0.0365760072950221 240 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS|MITOCHONDRION Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies aerobic respiration molecular_function mitochondrion|nucleus|cytoplasm YDR005C YDR005C MAF1 hom Verified 1.89671821740877 0.0289325690181104 no 283 0.040707541261929 211 NA FT NUCLEUS Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions negative regulation of transcription from RNA polymerase III promoter RNA polymerase III core binding nucleolus|nucleus|cytoplasm YFL034W_p YFL034W YFL034W hom Uncharacterized 1.88965429765954 0.0294021050863605 no 284 0.0311626319946502 214 NA Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk biological_process molecular_function integral to membrane YMR199W YMR199W CLN1 hom Verified 1.88584589325595 0.0296578622445117 no 285 0.0206652056407814 154 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of cyclin-dependent protein kinase activity cyclin-dependent protein kinase regulator activity nucleus|cytoplasm YNL190W_p YNL190W YNL190W hom Uncharacterized 1.88455655147149 0.029744866492232 no 286 0.0351753469275246 207 NA Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site cellular response to water deprivation molecular_function fungal-type cell wall YEL027W YEL027W VMA3 hom Verified 1.88076897962619 0.0300016758117182 no 287 0.0400186879684467 191 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis cellular copper ion homeostasis|cellular iron ion homeostasis|endocytosis|vacuole organization|proton transport|vacuolar acidification|protein targeting to vacuole hydrogen ion transmembrane transporter activity vacuolar proton-transporting V-type ATPase, V0 domain|integral to membrane YCL055W YCL055W KAR4 hom Verified 1.88073527986529 0.0300039689880993 no 288 0.0254134157234305 176 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone karyogamy involved in conjugation with cellular fusion|meiosis|negative regulation of transcription from RNA polymerase II promoter by pheromones|positive regulation of transcription from RNA polymerase II promoter by pheromones|G1 phase of mitotic cell cycle sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YDR322C-A YDR322C-A TIM11 hom Verified 1.87008554466657 0.0307359697550412 no 289 0.0309856709566077 157 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase protein oligomerization|cristae formation|ATP synthesis coupled proton transport structural molecule activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YOR133W YOR133W EFT1 hom Verified 1.86780718765063 0.0308944756209229 no 290 0.0341987189476567 230 NA FT TRANSLATION|NUCLEOTIDE METABOLISM RIBOSOME Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication translational elongation translation elongation factor activity ribosome YDL123W YDL123W SNA4 hom Verified 1.86707864667333 0.0309453029340608 no 291 0.0376263687970253 111 NA Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated biological_process molecular_function fungal-type vacuole lumen|fungal-type vacuole membrane YIL011W YIL011W TIR3 hom Verified 1.86546768294599 0.0310579389857985 no 292 0.0229197252390552 136 NA Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth biological_process molecular_function fungal-type cell wall YHR139C YHR139C SPS100 hom Verified 1.86522896772352 0.0310746584002959 no 293 0.037650246270193 219 NA FT KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin ascospore wall assembly molecular_function ascospore wall YDL074C YDL074C BRE1 hom Verified 1.86316353250786 0.0312196309364542 no 294 0.0771653916309973 314 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control mitotic cell cycle G1/S transition DNA damage checkpoint|intra-S DNA damage checkpoint|meiotic DNA double-strand break formation|histone monoubiquitination|double-strand break repair via homologous recombination|transcription from RNA polymerase II promoter|chromatin silencing at telomere ubiquitin-protein ligase activity nuclear chromatin YDR286C_p YDR286C YDR286C hom Uncharacterized 1.85789995902597 0.031591611113052 no 295 0.0189203087291683 79 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site biological_process molecular_function cellular_component YDR402C YDR402C DIT2 hom Verified 1.85648824672665 0.0316919983769456 no 296 0.0357741178819259 144 NA FT CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s ascospore wall assembly oxidoreductase activity cellular_component YNL139C YNL139C THO2 hom Verified 1.84825833735372 0.0322824898548171 no 297 0.0530077654780358 226 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION NUCLEUS Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|transcription elongation from RNA polymerase II promoter DNA binding|nucleic acid binding THO complex part of transcription export complex|nucleus|nucleoplasmic THO complex YLR376C YLR376C PSY3 hom Verified 1.83572948934306 0.0331988371485038 no 298 0.0333275839881603 215 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C recombinational repair|error-free translesion synthesis molecular_function nucleus|Shu complex|cytoplasm YOR247W YOR247W SRL1 hom Verified 1.83553872613321 0.033212953278312 no 299 0.0367240879033024 176 NA FT CELL WALL ORG/BIOGENESIS SITE OF POLARIZED GROWTH Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication fungal-type cell wall organization|nucleobase-containing compound metabolic process molecular_function fungal-type cell wall|fungal-type vacuole|cytoplasm|cellular bud tip YGR080W YGR080W TWF1 hom Verified 1.83527835142544 0.0332322285111393 no 300 0.0262616439287547 137 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Twinfilin, highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton, composed of two cofilin-like regions, localizes actin monomers to sites of rapid filament assembly bipolar cellular bud site selection|actin polymerization or depolymerization|sequestering of actin monomers actin monomer binding mating projection tip|actin cortical patch YML042W YML042W CAT2 hom Verified 1.83520762706783 0.0332374657429938 no 301 0.0287507514298766 150 NA FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes carnitine metabolic process carnitine O-acetyltransferase activity mitochondrion|peroxisomal matrix|peroxisome YFR048W YFR048W RMD8 hom Verified 1.8206347068198 0.0343312016971095 no 302 0.0355696570632524 199 NA FT CELL CYCLE Cytosolic protein required for sporulation biological_process molecular_function cytoplasm YAR014C YAR014C BUD14 hom Verified 1.81872272969482 0.0344768710911465 no 303 0.030885097824814 252 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress regulation of cell shape during vegetative growth phase|regulation of protein localization|regulation of transcription, DNA-dependent|cytoskeleton organization|negative regulation of actin filament polymerization protein phosphatase type 1 regulator activity incipient cellular bud site|cellular bud neck|cellular bud tip YPL152W YPL152W RRD2 hom Verified 1.81830613785898 0.0345086776029355 no 304 0.0317969242290188 226 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress mitotic spindle organization in nucleus|response to osmotic stress peptidyl-prolyl cis-trans isomerase activity|protein phosphatase type 2A regulator activity protein phosphatase type 2A complex YJL153C YJL153C INO1 hom Verified 1.81565921885548 0.0347113317291822 no 305 0.0409073562770387 177 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element inositol biosynthetic process inositol-3-phosphate synthase activity cytoplasm YGR184C YGR184C UBR1 hom Verified 1.8113891794532 0.0350403148776891 no 306 0.043351735257449 218 NA FT PROTEOLYSIS E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) regulation of dipeptide transport|ubiquitin-dependent protein catabolic process via the N-end rule pathway|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process|protein polyubiquitination ubiquitin-protein ligase activity proteasome regulatory particle, base subcomplex|cytoplasm YGR087C YGR087C PDC6 hom Verified 1.80675364715751 0.0354003494735713 no 307 0.0312838880451075 180 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation L-phenylalanine catabolic process|ethanol metabolic process|tryptophan catabolic process|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway pyruvate decarboxylase activity cytoplasm YFL047W YFL047W RGD2 hom Verified 1.80547689815718 0.035500043694823 no 308 0.0367536626556651 195 NA FT SIGNALING SITE OF POLARIZED GROWTH GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress small GTPase mediated signal transduction Rho GTPase activator activity cellular bud neck|cytoplasm YER097W_d YER097W YER097W hom Dubious 1.8003305443958 0.0359042303429436 no 309 0.0550088954680568 261 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR122C_d YMR122C YMR122C hom Dubious 1.79854699834932 0.0360451839262787 no 310 0.0554108441197531 203 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL227W YGL227W VID30 hom Verified 1.79557651652689 0.0362809467357104 no 311 0.0363956922838282 181 NA FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS NUCLEUS Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm regulation of nitrogen utilization|negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|vacuolar protein catabolic process molecular_function nucleus|GID complex|cytoplasm YLR342W YLR342W FKS1 hom Verified 1.79388391651156 0.0364158495403054 no 312 0.0375206165550218 211 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM MITOCHONDRION|PLASMA MEMBRANE|CYTOSKELETON Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling (1->3)-beta-D-glucan biosynthetic process|positive regulation of endocytosis|regulation of cell size 1,3-beta-D-glucan synthase activity mitochondrion|1,3-beta-D-glucan synthase complex|integral to membrane|plasma membrane|cellular bud neck|cellular bud tip|actin cortical patch YOR380W YOR380W RDR1 hom Verified 1.7923746108531 0.036536489478766 no 313 0.0356540830236117 178 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins response to xenobiotic stimulus sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YMR099C YMR099C YMR099C hom Verified 1.78976801331926 0.0367456066854566 no 314 0.047145284391796 261 NA FT CARBOHYDRATE METABOLISM NUCLEUS Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS cellular carbohydrate metabolic process glucose-6-phosphate 1-epimerase activity nucleus|cytoplasm YPR118W YPR118W MRI1 hom Verified 1.78910903021391 0.0367986290762653 no 315 0.0441706551081262 166 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway L-methionine salvage from methylthioadenosine S-methyl-5-thioribose-1-phosphate isomerase activity nucleus|cytoplasm YDR467C_d YDR467C YDR467C hom Dubious 1.78908796808623 0.0368003247858345 no 316 0.0421993684087649 185 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER004W YER004W FMP52 hom Verified 1.78772156556983 0.0369104703165038 no 317 0.0453098037627061 193 NA FT ENDOPLASMIC RETICULUM|MITOCHONDRION Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation biological_process molecular_function mitochondrial outer membrane|mitochondrion|endoplasmic reticulum YOR028C YOR028C CIN5 hom Verified 1.78677125589745 0.0369872334571484 no 318 0.025929147664246 130 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication hyperosmotic salinity response|response to drug|hyperosmotic response|regulation of transcription from RNA polymerase II promoter repressing transcription factor binding|sequence-specific DNA binding nucleus|cytoplasm YOR155C YOR155C ISN1 hom Verified 1.78633399885694 0.0370225975703516 no 319 0.0340362720792935 114 NA FT NUCLEOTIDE METABOLISM Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms nicotinamide riboside biosynthetic process|inosine salvage|nicotinic acid riboside biosynthetic process IMP 5'-nucleotidase activity cellular_component YGL053W YGL053W PRM8 hom Verified 1.77943365388269 0.0375843465561296 no 320 0.0304575555467117 176 NA FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family biological_process molecular_function integral to membrane|endoplasmic reticulum|plasma membrane YHR210C_p YHR210C YHR210C hom Uncharacterized 1.77831265633029 0.0376762594174643 no 321 0.0359292096203487 232 NA FT CARBOHYDRATE METABOLISM Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions biological_process molecular_function cellular_component YKL023W_p YKL023W YKL023W hom Uncharacterized 1.77293040111693 0.0381201201770213 no 322 0.0323541461371388 234 NA Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YML004C YML004C GLO1 hom Verified 1.7709913935074 0.0382810664612379 no 323 0.0401266599433062 205 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress methylglyoxal catabolic process to D-lactate|glutathione metabolic process lactoylglutathione lyase activity nucleus|cytoplasm YFR057W_p YFR057W YFR057W hom Uncharacterized 1.7680359737626 0.038527444936141 no 324 0.0459871911044306 183 NA Putative protein of unknown function biological_process molecular_function cellular_component YHR183W YHR183W GND1 hom Verified 1.76516674100062 0.0387678732464423 no 325 0.0404361757647529 218 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication cellular response to oxidative stress|pentose-phosphate shunt, oxidative branch phosphogluconate dehydrogenase (decarboxylating) activity mitochondrion|cytoplasm YIL035C YIL035C CKA1 hom Verified 1.76507666887088 0.0387754406115898 no 326 0.0465546806225426 253 NA FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein serine/threonine kinase activity|protein kinase activity protein kinase CK2 complex|UTP-C complex YGR219W_d YGR219W YGR219W hom Dubious 1.76495071171245 0.0387860248572956 no 327 0.0547790439389175 202 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Unknown Unknown Unknown YER186C_p YER186C YER186C hom Uncharacterized 1.762141912481 0.0390226617136126 no 328 0.0402715958537488 218 NA Putative protein of unknown function biological_process molecular_function cellular_component YEL072W YEL072W RMD6 hom Verified 1.76087369884328 0.0391298912072303 no 329 0.0405184582186935 193 NA FT CELL CYCLE Protein required for sporulation biological_process molecular_function cellular_component YJR055W YJR055W HIT1 hom Verified 1.75989290913878 0.0392129829166459 no 330 0.0261650995562217 203 NA FT NUCLEUS Protein of unknown function, required for growth at high temperature biological_process molecular_function nucleus|cytoplasm YMR022W YMR022W UBC7 hom Verified 1.75257741778036 0.0398372851643703 no 331 0.0538357798113967 222 NA FT CHROMATIN ORGANIZATION|CELL WALL ORG/BIOGENESIS|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly fungal-type cell wall organization|chromatin assembly or disassembly|ER-associated protein catabolic process ubiquitin-protein ligase activity endoplasmic reticulum membrane YGR279C YGR279C SCW4 hom Verified 1.74904729592086 0.0401414221635809 no 332 0.0425610488735582 204 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating conjugation with cellular fusion glucosidase activity extracellular region|fungal-type cell wall YGR121W-A_p YGR121W-A YGR121W-A hom Uncharacterized 1.74781844984015 0.0402477347521231 no 333 0.0355722194253363 186 NA Putative protein of unknown function biological_process molecular_function cellular_component YMR289W YMR289W ABZ2 hom Verified 1.74502646052298 0.0404901315573376 no 334 0.0471167934309632 246 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis folic acid biosynthetic process 4-amino-4-deoxychorismate lyase activity cytoplasm YKL217W YKL217W JEN1 hom Verified 1.74205816894741 0.0407491331350559 no 335 0.0403564980405594 190 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose plasma membrane lactate transport|plasma membrane selenite transport|plasma membrane pyruvate transport selenite:hydrogen symporter activity|secondary active monocarboxylate transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YOR337W YOR337W TEA1 hom Verified 1.73901050829624 0.0410164572767578 no 336 0.0417714362023932 245 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein transcription, DNA-dependent sequence-specific DNA binding|DNA binding nucleus YOR134W YOR134W BAG7 hom Verified 1.73382409955863 0.0414746491241701 no 337 0.0484937738176887 257 NA FT SIGNALING Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p small GTPase mediated signal transduction Rho GTPase activator activity intracellular YML050W YML050W AIM32 hom Verified 1.73042159568355 0.0417774890975874 no 338 0.053713917131608 228 NA Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss biological_process molecular_function cellular_component YGR053C_p YGR053C YGR053C hom Uncharacterized 1.72985115631309 0.0418284359226682 no 339 0.0407165257392511 188 NA Putative protein of unknown function biological_process molecular_function cellular_component YNL179C_d YNL179C YNL179C hom Dubious 1.72782876918481 0.0420094639446197 no 340 0.0419517883382542 229 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YLR237W YLR237W THI7 hom Verified 1.72738308321468 0.0420494433703994 no 341 0.0590739378329045 189 NA FT PLASMA MEMBRANE Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia thiamine transport thiamine transmembrane transporter activity integral to membrane|plasma membrane YCL011C YCL011C GBP2 hom Verified 1.72076550609008 0.042646692045444 no 342 0.0569229739097829 253 NA FT RNA LOCALIZATION NUCLEUS|CHROMOSOME Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication poly(A)+ mRNA export from nucleus|telomere maintenance RNA binding|telomeric DNA binding nucleus|cytoplasmic stress granule YPR049C YPR049C ATG11 hom Verified 1.72054151304842 0.0426670272217269 no 343 0.0526081984496596 260 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|PEROXISOME ORGANIZATION Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) piecemeal microautophagy of nucleus|mitochondrion degradation|peroxisome degradation|protein localization to pre-autophagosomal structure|CVT pathway protein complex scaffold pre-autophagosomal structure|extrinsic to membrane YBR277C_d YBR277C YBR277C hom Dubious 1.71992253882025 0.0427232614729567 no 344 0.0439773061408453 254 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Unknown Unknown Unknown YFL032W_d YFL032W YFL032W hom Dubious 1.71788254577991 0.0429090205419387 no 345 0.0529287321280937 263 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Unknown Unknown Unknown YCL005W YCL005W LDB16 hom Verified 1.71722376372296 0.0429691475931925 no 346 0.0602069162096196 307 NA FT MITOCHONDRION Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria biological_process molecular_function mitochondrion|lipid particle YKL073W YKL073W LHS1 hom Verified 1.71477671967409 0.0431930856769137 no 347 0.0610089766685349 302 NA FT PROTEIN LOCALIZATION|NUCLEOTIDE METABOLISM ENDOPLASMIC RETICULUM Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway posttranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|adenyl-nucleotide exchange factor activity|ATP binding endoplasmic reticulum lumen YGL228W YGL228W SHE10 hom Verified 1.71409408608371 0.0432557238805601 no 348 0.0409506749808241 216 NA FT MITOCHONDRION Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest biological_process molecular_function cellular_component YGL133W YGL133W ITC1 hom Verified 1.71129769613297 0.0435130857250839 no 349 0.0507500077056984 232 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex negative regulation of transcription from RNA polymerase II promoter by pheromones|chromatin remodeling|chromatin silencing at telomere molecular_function chromatin accessibility complex YFR009W YFR009W GCN20 hom Verified 1.7078594312264 0.0438312141701835 no 350 0.0821408844058857 427 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|NUCLEOTIDE METABOLISM RIBOSOME Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA regulation of translational elongation ATPase activity cytosol|cytosolic ribosome YBR297W YBR297W MAL33 hom Verified 1.7035082075975 0.0442365016758037 no 351 0.0442252947482237 221 NA FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM NUCLEUS MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C cellular carbohydrate metabolic process|regulation of transcription, DNA-dependent sequence-specific DNA binding transcription factor activity nucleus YOL072W YOL072W THP1 hom Verified 1.70233735166807 0.0443460733509398 no 352 0.0864516121489262 378 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING|RESPONSE TO DNA DAMAGE|CELL DIVISION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding mRNA export from nucleus|mRNA 3'-end processing|transcription-coupled nucleotide-excision repair|cellular bud site selection|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|transcription elongation from RNA polymerase II promoter double-stranded DNA binding|RNA binding nuclear envelope|nucleus|transcription export complex 2|nuclear pore YKL136W_d YKL136W YKL136W hom Dubious 1.69889525914262 0.0446694602219182 no 353 0.0623615869455983 240 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Unknown Unknown Unknown YGR269W_d YGR269W YGR269W hom Dubious 1.69468616987645 0.04506748532105 no 354 0.0421803716843852 241 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Unknown Unknown Unknown YPL001W YPL001W HAT1 hom Verified 1.69327780171279 0.0452013007051786 no 355 0.0565981467370661 220 NA FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS HISTONE ACETYLTRANSFERASE Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair histone acetylation|chromatin silencing at telomere histone acetyltransferase activity|histone binding histone acetyltransferase complex|nucleus|cytoplasm YDR182W-A_p YDR182W-A YDR182W-A hom Uncharacterized 1.69054788369899 0.0454615927806354 no 356 0.0540894685840905 220 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YMR166C_p YMR166C YMR166C hom Uncharacterized 1.68996297273345 0.0455175193420599 no 357 0.0523091981932841 276 NA FT MITOCHONDRION Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene transport transporter activity integral to membrane|mitochondrial inner membrane YER132C YER132C PMD1 hom Verified 1.6831377703427 0.0461742120853595 no 358 0.0506588884199104 248 NA FT CELL CYCLE Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions sporulation resulting in formation of a cellular spore molecular_function cytoplasm YMR031C YMR031C EIS1 hom Verified 1.67883136724232 0.0465924567588165 no 359 0.0528638611702859 212 NA FT PROTEIN COMPLEX ORG/BIOGENESIS MITOCHONDRION|PLASMA MEMBRANE Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress eisosome assembly molecular_function eisosome|mitochondrion|membrane raft|cytoplasm YGR226C_d YGR226C YGR226C hom Dubious 1.67670887258048 0.0467997123813716 no 360 0.0497775634483926 200 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Unknown Unknown Unknown YIL005W YIL005W EPS1 hom Verified 1.6710867376716 0.0473522732831281 no 361 0.0457670624568735 277 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family protein retention in ER lumen|ER-associated protein catabolic process unfolded protein binding|protein disulfide isomerase activity|chaperone binding endoplasmic reticulum membrane YOL014W_p YOL014W YOL014W hom Uncharacterized 1.66766102650316 0.0476915181375098 no 362 0.0616014494455509 245 NA Putative protein of unknown function biological_process molecular_function cellular_component YHR151C YHR151C MTC6 hom Verified 1.66654835800568 0.0478021224402654 no 363 0.0457701449685851 259 NA Protein of unknown function; mtc6 is synthetically sick with cdc13-1 biological_process molecular_function cellular_component YLR126C YLR126C YLR126C hom Verified 1.66351379177669 0.0481048162231729 no 364 0.0402870056000052 184 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress cellular copper ion homeostasis|cellular iron ion homeostasis molecular_function cytoplasm YBR151W YBR151W APD1 hom Verified 1.65947298982667 0.0485102594980588 no 365 0.0444086951169499 264 NA FT NUCLEUS Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YBL060W YBL060W YEL1 hom Verified 1.65871622642802 0.0485864940427618 no 366 0.0552585823239743 256 NA FT PROTEIN LOCALIZATION|LIPID METABOLISM|SIGNALING SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip positive regulation of phosphatidylinositol biosynthetic process|protein localization|actin cortical patch localization ARF guanyl-nucleotide exchange factor activity cellular bud neck|cellular bud tip|cytoplasm YJL077W-B_p YJL077W-B YJL077W-B hom Uncharacterized 1.65768468838973 0.048690562963015 no 367 0.07426137049394 231 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YER078C YER078C ICP55 hom Verified 1.65721045250753 0.048738467015241 no 368 0.0464159310609438 206 NA FT PROTEOLYSIS NUCLEUS|MITOCHONDRION Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family protein stabilization|protein processing aminopeptidase activity extrinsic to mitochondrial inner membrane|mitochondrion|nucleus YGR263C YGR263C SAY1 hom Verified 1.65377080356581 0.0490870451501191 no 369 0.0531078415309486 202 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum response to toxin|sterol metabolic process|sterol deacetylation steryl deacetylase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum lumen YGR111W_p YGR111W YGR111W hom Uncharacterized 1.65285036072216 0.0491806608865395 no 370 0.0700030182973816 319 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus regulation of cell size molecular_function nucleus|cytoplasm YMR057C_d YMR057C YMR057C hom Dubious 1.65057721271657 0.0494124675225403 no 371 0.0446010447697733 228 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Unknown Unknown Unknown YGR008C YGR008C STF2 hom Verified 1.64574550238033 0.0499080833062875 no 372 0.0479502769915902 173 NA FT MITOCHONDRION Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress cellular response to desiccation molecular_function cytoplasm YKL076C_d YKL076C PSY1 hom Dubious 1.64407784095403 0.0500800622486408 no 373 0.0526541709280957 270 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Unknown Unknown Unknown YGL148W YGL148W ARO2 hom Verified 1.64293136595162 0.0501985672503082 no 374 0.123853719052732 515 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress aromatic amino acid family biosynthetic process chorismate synthase activity|riboflavin reductase (NADPH) activity cytoplasm YAL029C YAL029C MYO4 hom Verified 1.64069310492738 0.0504305684696396 no 375 0.0482184863488919 222 NA FT RNA LOCALIZATION MITOCHONDRION|SITE OF POLARIZED GROWTH|CYTOSKELETON Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication mating type switching|endoplasmic reticulum inheritance|intracellular mRNA localization microfilament motor activity|actin filament binding mitochondrion|filamentous actin|myosin V complex|cellular bud|cellular bud tip YOR073W YOR073W SGO1 hom Verified 1.6405320327188 0.0504472968957233 no 376 0.0653910955397716 265 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability positive regulation of maintenance of meiotic sister chromatid cohesion|meiotic sister chromatid segregation|meiotic sister chromatid separation|sister chromatid biorientation|maintenance of meiotic sister chromatid cohesion|mitotic cell cycle spindle assembly checkpoint|mitotic sister chromatid segregation molecular_function condensed nuclear chromosome kinetochore|nucleus|spindle pole body|condensed nuclear chromosome, centromeric region YOL140W YOL140W ARG8 hom Verified 1.63942171977332 0.0505627306331851 no 377 0.0366606791018866 175 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine arginine biosynthetic process|ornithine biosynthetic process N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity mitochondrion|mitochondrial matrix YBR128C YBR128C ATG14 hom Verified 1.63122886567948 0.0514210183213929 no 378 0.0467779154096768 241 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor piecemeal microautophagy of nucleus|ER-associated protein catabolic process|peroxisome degradation|macroautophagy|CVT pathway 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase complex I|fungal-type vacuole membrane|pre-autophagosomal structure|extrinsic to membrane YKR011C YKR011C YKR011C hom Verified 1.63109047271232 0.0514356153466944 no 379 0.0426620625343074 220 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YEL023C_p YEL023C YEL023C hom Uncharacterized 1.63104432321886 0.0514404837062018 no 380 0.051736436342525 220 NA Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene biological_process molecular_function cellular_component YNL144C_p YNL144C YNL144C hom Uncharacterized 1.63014318384142 0.0515356193274824 no 381 0.0424257132023997 213 NA FT MITOCHONDRION Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study biological_process molecular_function mitochondrion YMR237W YMR237W BCH1 hom Verified 1.62408920263559 0.0521783860190014 no 382 0.0587146717623875 302 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM GOLGI APPARATUS Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process molecular_function ribosome|clathrin-coated vesicle|exomer complex YPR022C_p YPR022C YPR022C hom Uncharacterized 1.62230182576209 0.0523693689840771 no 383 0.0585241272732165 220 NA FT NUCLEUS Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process sequence-specific DNA binding nucleus|cytoplasm YMR167W YMR167W MLH1 hom Verified 1.62202405432045 0.05239909889953 no 384 0.0355567092913219 186 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION|CHROMOSOME Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer mismatch repair|meiotic mismatch repair|reciprocal meiotic recombination|meiotic heteroduplex formation double-stranded DNA binding|DNA insertion or deletion binding|loop DNA binding|single-stranded DNA binding|dinucleotide insertion or deletion binding|ATPase activity|ATP binding mitochondrion|nucleus|MutLalpha complex|MutLbeta complex|cytoplasm YPR021C YPR021C AGC1 hom Verified 1.62176051519452 0.0524273179139887 no 385 0.056651694065321 302 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis L-glutamate transport|aspartate transport|cellular nitrogen compound biosynthetic process antiporter activity|L-aspartate transmembrane transporter activity|L-glutamate transmembrane transporter activity|uniporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YPL025C_d YPL025C YPL025C hom Dubious 1.62152024305155 0.0524530560792785 no 386 0.0578052937159363 263 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL127C YPL127C HHO1 hom Verified 1.62042443603878 0.0525705671154696 no 387 0.0431342949686465 208 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination negative regulation of DNA recombination|negative regulation of chromatin silencing|regulation of transcription, DNA-dependent DNA binding nucleus|nuclear nucleosome YHR087W YHR087W RTC3 hom Verified 1.61962912577632 0.0526559845669618 no 388 0.0743974356589424 351 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress RNA metabolic process molecular_function nucleus|cytoplasm YDR533C YDR533C HSP31 hom Verified 1.61550540437538 0.0531006454677425 no 389 0.0765774116158775 314 NA FT PROTEOLYSIS Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress biological_process unfolded protein binding|cysteine-type peptidase activity cellular_component YMR172C-A_d YMR172C-A YMR172C-A hom Dubious 1.61542558942839 0.0531092811945145 no 390 0.0520744777417676 264 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL008W-A_p YBL008W-A YBL008W-A hom Uncharacterized 1.61433300490003 0.0532276074007549 no 391 0.0489270690827775 302 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGR289C YGR289C MAL11 hom Verified 1.61425354979162 0.0532362204832064 no 392 0.0671253639437992 313 NA FT CARBOHYDRATE METABOLISM PLASMA MEMBRANE Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization trehalose transport|maltose transport|disaccharide catabolic process trehalose transmembrane transporter activity|alpha-glucoside:hydrogen symporter activity|maltose:hydrogen symporter activity integral to membrane|plasma membrane YPR114W_p YPR114W YPR114W hom Uncharacterized 1.60798614795176 0.0539191062974102 no 393 0.063439028042123 277 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative protein of unknown function biological_process molecular_function integral to membrane|endoplasmic reticulum YMR169C YMR169C ALD3 hom Verified 1.60797846283347 0.0539199478924657 no 394 0.0542897260087353 256 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose beta-alanine biosynthetic process|polyamine catabolic process aldehyde dehydrogenase (NAD) activity cytoplasm YOR163W YOR163W DDP1 hom Verified 1.60708904606476 0.0540174179340534 no 395 0.0419275743504536 202 NA FT CARBOHYDRATE METABOLISM NUCLEUS Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress polyphosphate catabolic process|inositol phosphate dephosphorylation|diadenosine polyphosphate catabolic process diphosphoinositol-polyphosphate diphosphatase activity|endopolyphosphatase activity|bis(5'-adenosyl)-pentaphosphatase activity|bis(5'-adenosyl)-hexaphosphatase activity nucleus|cytoplasm YPL216W_p YPL216W YPL216W hom Uncharacterized 1.60695765384576 0.0540318288552052 no 396 0.0529454108260529 257 NA FT CHROMATIN ORGANIZATION NUCLEUS Putative protein of unknown function; YPL216W is not an essential gene biological_process molecular_function cellular_component YDR275W YDR275W BSC2 hom Verified 1.60550372212602 0.0541914973552404 no 397 0.0524514697856865 226 NA Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication biological_process molecular_function lipid particle YPL258C YPL258C THI21 hom Verified 1.60488798946706 0.0542592285936862 no 398 0.0478537656796213 256 NA Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p thiamine biosynthetic process phosphomethylpyrimidine kinase activity|hydroxymethylpyrimidine kinase activity cellular_component YBR046C YBR046C ZTA1 hom Verified 1.60188146293149 0.0545909121878371 no 399 0.057087501429912 250 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS NUCLEUS NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin cellular response to oxidative stress NADPH:quinone reductase activity|AU-rich element binding|2-alkenal reductase [NAD(P)] activity nucleus|cytoplasm YKR015C_p YKR015C YKR015C hom Uncharacterized 1.59736205501494 0.055092512785 no 400 0.0368911292866468 188 NA Putative protein of unknown function biological_process molecular_function cellular_component YKR089C YKR089C TGL4 hom Verified 1.5967684958561 0.0551586604525255 no 401 0.056221661388365 244 NA FT LIPID METABOLISM|CELL DIVISION Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p phospholipid metabolic process|triglyceride catabolic process|triglyceride mobilization|cell budding lysophosphatidic acid acyltransferase activity|triglyceride lipase activity|calcium-independent phospholipase A2 activity|sterol esterase activity lipid particle YPL101W YPL101W ELP4 hom Verified 1.5883239989575 0.0561065467064159 no 402 0.0801948316688782 329 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity regulation of transcription from RNA polymerase II promoter|tRNA wobble uridine modification ATPase activity|tRNA binding Elongator holoenzyme complex|cytoplasm YPL172C YPL172C COX10 hom Verified 1.58615736895172 0.0563518078453812 no 403 0.0772895080448508 376 NA FT COFACTOR METABOLISM MITOCHONDRION Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders heme a biosynthetic process protoheme IX farnesyltransferase activity mitochondrion|integral to membrane YNL176C_p YNL176C TDA7 hom Uncharacterized 1.58609760225848 0.0563585853555531 no 404 0.0552769830234211 227 NA Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function fungal-type vacuole YLL002W YLL002W RTT109 hom Verified 1.58257709764817 0.0567589434732988 no 405 0.0625092944365191 268 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p negative regulation of transposition, RNA-mediated|histone acetylation|double-strand break repair via nonhomologous end joining|response to DNA damage stimulus|regulation of transcription from RNA polymerase II promoter in response to stress H3 histone acetyltransferase activity nucleus YNL336W YNL336W COS1 hom Verified 1.5818482074533 0.056842113427688 no 406 0.0604916194707455 269 NA Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function integral to membrane|fungal-type vacuole YKR045C_p YKR045C YKR045C hom Uncharacterized 1.57437900299367 0.0576999275456582 no 407 0.0578968511141335 264 NA Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm biological_process molecular_function cytoplasm YOR201C YOR201C MRM1 hom Verified 1.57415166495684 0.0577261953457498 no 408 0.0655461479854224 330 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS MITOCHONDRION Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA rRNA modification rRNA (guanine-N1-)-methyltransferase activity mitochondrion YGR168C_p YGR168C YGR168C hom Uncharacterized 1.57155785126074 0.0580265639424421 no 409 0.0883855178586347 356 NA Putative protein of unknown function; YGR168C is not an essential gene biological_process molecular_function integral to membrane YHL005C_d YHL005C YHL005C hom Dubious 1.57113787951087 0.0580753128051142 no 410 0.0442445452139638 221 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Unknown Unknown Unknown YOL075C_p YOL075C YOL075C hom Uncharacterized 1.56871903157104 0.0583567110180331 no 411 0.0312689162190559 148 NA FT NUCLEOTIDE METABOLISM Putative ABC transporter transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YHR189W YHR189W PTH1 hom Verified 1.56845742703705 0.0583872090118692 no 412 0.0535972701316932 285 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium mitochondrial translation aminoacyl-tRNA hydrolase activity mitochondrion YDR008C_d YDR008C YDR008C hom Dubious 1.56708832932441 0.0585470233699828 no 413 0.221233058445723 874 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL152W YOL152W FRE7 hom Verified 1.56651339204488 0.0586142379355854 no 414 0.0602445785192941 245 NA FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY PLASMA MEMBRANE Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YOL117W YOL117W RRI2 hom Verified 1.5659640323235 0.0586785188708377 no 415 0.0606162803517508 305 NA FT SIGNALING NUCLEUS Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YCR077C YCR077C PAT1 hom Verified 1.5638228985979 0.0589295825755317 no 416 0.0719547221224334 383 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|TRANSLATION|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS RIBOSOME Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|chromosome segregation|regulation of translational initiation|formation of translation preinitiation complex|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly molecular_function cytosolic small ribosomal subunit|cytoplasmic mRNA processing body YKL053C-A YKL053C-A MDM35 hom Verified 1.55963539348601 0.0594230337675315 no 417 0.0679722217339223 293 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION NUCLEUS|MITOCHONDRION Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress mitochondrion organization|mitochondrial respiratory chain complex assembly molecular_function nucleus|cytoplasm|mitochondrial intermembrane space YLR250W YLR250W SSP120 hom Verified 1.55634205999081 0.0598133874579819 no 418 0.0576437851234353 232 NA Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm YGL082W_p YGL082W YGL082W hom Uncharacterized 1.54920595743896 0.0606661089115942 no 419 0.0406595344620948 203 NA FT NUCLEUS Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene biological_process molecular_function nucleus|plasma membrane|cytoplasm YBR041W YBR041W FAT1 hom Verified 1.54703133761698 0.0609278446080244 no 420 0.0839008409645001 321 NA FT KETONE METABOLISM|LIPID METABOLISM ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids very long-chain fatty acid metabolic process|long-chain fatty acid transport very long-chain fatty acid-CoA ligase activity|long-chain fatty acid transporter activity intrinsic to internal side of plasma membrane|integral to membrane|endoplasmic reticulum|lipid particle|peroxisome YPL272C_p YPL272C YPL272C hom Uncharacterized 1.5452641761984 0.0611411884241489 no 421 0.0808693891552772 356 NA Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene biological_process molecular_function cellular_component YCL028W YCL028W RNQ1 hom Verified 1.5430654417764 0.0614074492770071 no 422 0.0474726181203367 249 NA FT NUCLEUS [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate biological_process molecular_function cytosol YBR013C_p YBR013C YBR013C hom Uncharacterized 1.54164430541888 0.0615800264277414 no 423 0.0682131068129372 370 NA Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YLR294C_d YLR294C YLR294C hom Dubious 1.53957349230803 0.0618321754923564 no 424 0.0638568094343319 283 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Unknown Unknown Unknown YCL026C-B YCL026C-B HBN1 hom Verified 1.5390331899614 0.0618980968828798 no 425 0.0715706294741041 317 NA FT OXIDATION-REDUCTION PROCESS NUCLEUS Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress biological_process NAD(P)H nitroreductase activity nucleus|cytoplasm YDR524C-B_p YDR524C-B YDR524C-B hom Uncharacterized 1.53778874909854 0.0620501376973844 no 426 0.0893199638253746 385 NA FT PLASMA MEMBRANE Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication biological_process molecular_function cellular_component YNL120C_d YNL120C YNL120C hom Dubious 1.53306170963444 0.062630325467446 no 427 0.0524166080708127 316 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Unknown Unknown Unknown YBR159W YBR159W IFA38 hom Verified 1.52899983261195 0.0631322427078125 no 428 0.0630986491193961 279 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides fatty acid elongation ketoreductase activity integral to membrane|endoplasmic reticulum YBR072C-A_p YBR072C-A YBR072C-A hom Uncharacterized 1.52623597672663 0.0634755533513414 no 429 0.0586163384803733 263 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YGL062W YGL062W PYC1 hom Verified 1.5240386840053 0.063749524031973 no 430 0.055756748279392 269 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication NADPH regeneration|gluconeogenesis pyruvate carboxylase activity cytosol YBR095C YBR095C RXT2 hom Verified 1.51754266197922 0.0645648654244939 no 431 0.0937630694039446 389 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth conjugation with cellular fusion|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of transcription from RNA polymerase II promoter|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA|invasive growth in response to glucose limitation histone deacetylase activity Rpd3L-Expanded complex|nucleus|Rpd3L complex YHR028C YHR028C DAP2 hom Verified 1.51464518233181 0.0649311417666195 no 432 0.0836985682975239 300 NA FT PROTEOLYSIS Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p protein processing dipeptidyl-peptidase activity fungal-type vacuole membrane YPR157W_p YPR157W TDA6 hom Uncharacterized 1.51459041275788 0.0649380808018568 no 433 0.0711467782989802 299 NA FT PROTEIN LOCALIZATION Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function cellular_component YLR128W YLR128W DCN1 hom Verified 1.51373044463468 0.0650471100226784 no 434 0.0781922609660262 287 NA Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation protein neddylation|positive regulation of ubiquitin-protein ligase activity ubiquitin binding|NEDD8 ligase activity|small conjugating protein binding cellular_component YIR036C_p YIR036C IRC24 hom Uncharacterized 1.51252197353926 0.0652005634882333 no 435 0.0667467994170991 292 NA FT OXIDATION-REDUCTION PROCESS Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci biological_process molecular_function integral to membrane|cytoplasm YDL100C YDL100C GET3 hom Verified 1.5091495843484 0.0656302803066835 no 436 0.129142483811764 495 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM ENDOPLASMIC RETICULUM|GOLGI APPARATUS Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress retrograde vesicle-mediated transport, Golgi to ER|pheromone-dependent signal transduction involved in conjugation with cellular fusion|response to heat|protein insertion into ER membrane|response to metal ion|posttranslational protein targeting to membrane ATPase activity|guanyl-nucleotide exchange factor activity endoplasmic reticulum|GET complex YDL080C YDL080C THI3 hom Verified 1.50791024049336 0.0657887506820267 no 437 0.0546564334200402 233 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis regulation of thiamine biosynthetic process|branched-chain amino acid catabolic process to alcohol via Ehrlich pathway|positive regulation of transcription from RNA polymerase II promoter|leucine catabolic process carboxy-lyase activity cytosol|nucleus YJL038C YJL038C LOH1 hom Verified 1.50657430212478 0.0659599042395716 no 438 0.0676142866371083 295 NA Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 chromosome organization molecular_function cellular_component YGR044C YGR044C RME1 hom Verified 1.50482778992033 0.0661841785404119 no 439 0.0213603556215142 147 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION NUCLEUS Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription during meiosis|negative regulation of meiosis|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity nucleus YCR085W_d YCR085W YCR085W hom Dubious 1.50292894225166 0.0664286843518603 no 440 0.0634483948060681 349 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL038W-A_p YPL038W-A YPL038W-A hom Uncharacterized 1.50230922816946 0.0665086332488739 no 441 0.047322868795178 229 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHR129C YHR129C ARP1 hom Verified 1.50131638786731 0.066636874144299 no 442 0.0530668760363681 322 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION CYTOSKELETON Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin nuclear migration|establishment of mitotic spindle orientation structural constituent of cytoskeleton cell cortex|dynactin complex|spindle pole body|astral microtubule YBL011W YBL011W SCT1 hom Verified 1.49968691573551 0.0668477607125204 no 443 0.0672956458671423 333 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed phospholipid biosynthetic process glycerol-3-phosphate O-acyltransferase activity|glycerone-phosphate O-acyltransferase activity integral to membrane|endoplasmic reticulum YER088C YER088C DOT6 hom Verified 1.4983067275164 0.0670267884925796 no 444 0.0700737760405186 368 NA FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING NUCLEUS|CHROMOSOME Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress chromatin silencing at rDNA|filamentous growth|regulation of transcription from RNA polymerase II promoter|unidimensional cell growth|chromatin silencing at telomere sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|Rpd3L complex|cytoplasm YGR275W YGR275W RTT102 hom Verified 1.49582031304103 0.0673502432260242 no 445 0.0798771997696746 274 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS RSC COMPLEX Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress chromosome segregation|nucleosome disassembly|chromatin remodeling|transcription elongation from RNA polymerase II promoter molecular_function RSC complex|SWI/SNF complex|nucleus YDR536W YDR536W STL1 hom Verified 1.49529213683817 0.067419108167447 no 446 0.0717787959696611 324 NA FT PLASMA MEMBRANE Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock transport|glycerol transport|transmembrane transport solute:hydrogen symporter activity|transporter activity integral to membrane|plasma membrane|membrane YLR420W YLR420W URA4 hom Verified 1.49508327528094 0.0674463550730212 no 447 0.0754869023450363 328 NA FT NUCLEOTIDE METABOLISM NUCLEUS Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate 'de novo' pyrimidine nucleobase biosynthetic process|pyrimidine nucleotide biosynthetic process dihydroorotase activity nucleus|cytoplasm YPR007C YPR007C REC8 hom Verified 1.49439189595917 0.0675366092506556 no 448 0.0693025495962481 256 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p synaptonemal complex assembly|protein localization to chromosome, centromeric region|reciprocal meiotic recombination|meiotic sister chromatid cohesion molecular_function condensed nuclear chromosome|condensed nuclear chromosome, centromeric region YBR119W YBR119W MUD1 hom Verified 1.49364534154815 0.0676341708709419 no 449 0.0857974991616511 314 NA FT RNA PROCESSING NUCLEUS U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing mRNA splicing, via spliceosome U1 snRNA binding|RNA binding U1 snRNP|U2-type prespliceosome YLR202C_d YLR202C YLR202C hom Dubious 1.49348796793919 0.0676547507415946 no 450 0.0636786684757874 311 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Unknown Unknown Unknown YDL035C YDL035C GPR1 hom Verified 1.49311116220537 0.0677040455829755 no 451 0.0894926950062564 353 NA FT SIGNALING PLASMA MEMBRANE Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis replicative cell aging|G-protein coupled receptor signaling pathway|sucrose mediated signaling|glucose mediated signaling pathway|pseudohyphal growth|hexose mediated signaling|invasive growth in response to glucose limitation|detection of glucose|detection of sucrose stimulus G-protein coupled receptor activity|glucose binding integral to membrane|plasma membrane YJL096W YJL096W MRPL49 hom Verified 1.49252892669136 0.0677802699105099 no 452 0.0891875029316843 351 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR271W_p YLR271W YLR271W hom Uncharacterized 1.48858825057609 0.0682979137279774 no 453 0.0550608780743243 218 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YGL095C YGL095C VPS45 hom Verified 1.48752883838201 0.068437596216398 no 454 0.16337432053951 636 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment vacuole inheritance|CVT pathway|positive regulation of SNARE complex assembly|Golgi vesicle fusion to target membrane|Golgi to vacuole transport|vacuole organization|vacuolar acidification|protein targeting to vacuole|Golgi to endosome transport unfolded protein binding|SNARE binding cytosol|SNARE complex|Golgi membrane|extrinsic to membrane YCR021C YCR021C HSP30 hom Verified 1.4867528745463 0.0685400460703721 no 455 0.0493819824624441 184 NA FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS PLASMA MEMBRANE Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase negative regulation of ATPase activity|response to stress molecular_function integral to membrane|plasma membrane YER143W YER143W DDI1 hom Verified 1.48587989769833 0.0686554458380371 no 456 0.0503638221655361 248 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS PLASMA MEMBRANE DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains vesicle-mediated transport|protein secretion|ubiquitin-dependent protein catabolic process ubiquitin binding|SNARE binding|aspartic-type endopeptidase activity plasma membrane YBR111W-A YBR111W-A SUS1 hom Verified 1.4807503338579 0.0693365578876575 no 457 0.104525685918608 387 NA FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE|HISTONE ACETYLTRANSFERASE Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP poly(A)+ mRNA export from nucleus|regulation of protein localization|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|histone deubiquitination|histone H3-K4 methylation|transcription elongation from RNA polymerase II promoter|histone H3-K79 methylation|positive regulation of transcription from RNA polymerase II promoter enzyme activator activity DUBm complex|nucleoplasm|transcription export complex 2|SAGA complex|nuclear pore YGR050C_p YGR050C YGR050C hom Uncharacterized 1.47900624277314 0.0695693231700488 no 458 0.0598081380374297 269 NA Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YCL063W YCL063W VAC17 hom Verified 1.47819249375756 0.0696781312327512 no 459 0.0760297910289875 352 NA Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p vacuole inheritance protein anchor fungal-type vacuole membrane YCR107W YCR107W AAD3 hom Verified 1.47810978326964 0.0696891979536297 no 460 0.0868165191353421 353 NA FT OXIDATION-REDUCTION PROCESS Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role cellular aldehyde metabolic process aryl-alcohol dehydrogenase (NAD+) activity cellular_component YDL134C YDL134C PPH21 hom Verified 1.47809443551096 0.0696912516432562 no 461 0.0519582809159752 253 NA FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication actin filament organization|budding cell bud growth|protein dephosphorylation|G1/S transition of mitotic cell cycle|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity protein phosphatase type 2A complex YKR013W YKR013W PRY2 hom Verified 1.47672243597772 0.069875027746162 no 462 0.095071344310597 385 NA Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication biological_process molecular_function extracellular region|fungal-type vacuole YGL164C YGL164C YRB30 hom Verified 1.47559985408004 0.0700256721687663 no 463 0.066308847596398 288 NA FT PROTEIN LOCALIZATION NUCLEUS RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes biological_process GTPase regulator activity nucleus|cytoplasm YNL303W_d YNL303W YNL303W hom Dubious 1.47517241805775 0.0700830974113093 no 464 0.0558491820321788 268 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBR233W YBR233W PBP2 hom Verified 1.47514619531112 0.0700866215681127 no 465 0.0734985926333694 271 NA FT NUCLEUS RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress telomere maintenance via telomere shortening mRNA binding cytoplasm YAL065C_p YAL065C YAL065C hom Uncharacterized 1.47384904907473 0.0702611193199095 no 466 0.0653685716741678 255 NA Putative protein of unknown function; has homology to FLO1; possible pseudogene biological_process molecular_function cellular_component YPR066W YPR066W UBA3 hom Verified 1.47240527945029 0.0704557341771135 no 467 0.0800533928652105 315 NA Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern protein neddylation NEDD8 activating enzyme activity cytoplasm YJR131W YJR131W MNS1 hom Verified 1.47086103994673 0.0706643504066448 no 468 0.0727894245197513 251 NA FT CARBOHYDRATE METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation ER-associated protein catabolic process|protein deglycosylation involved in glycoprotein catabolic process mannosyl-oligosaccharide 1,2-alpha-mannosidase activity endoplasmic reticulum YOL087C YOL087C DUF1 hom Verified 1.46610499701005 0.0713098423020158 no 469 0.126643413891094 437 NA Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid biological_process ubiquitin binding cytoplasm YDR151C YDR151C CTH1 hom Verified 1.46331856505467 0.0716901150388971 no 470 0.0760612961516229 315 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS NUCLEUS Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis nuclear-transcribed mRNA catabolic process|cellular iron ion homeostasis mRNA binding nucleus YMR307W YMR307W GAS1 hom Verified 1.46213776503292 0.0718517308791839 no 471 0.0790020201078284 383 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|GENE SILENCING NUCLEUS|MITOCHONDRION|PLASMA MEMBRANE Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery fungal-type cell wall organization|chromatin silencing|filamentous growth 1,3-beta-glucanosyltransferase activity mitochondrion|membrane raft|primary cell septum|nuclear periphery|integral to membrane|fungal-type cell wall|plasma membrane|cellular bud scar|ER to Golgi transport vesicle YKL146W YKL146W AVT3 hom Verified 1.46048436123816 0.0720785012521019 no 472 0.0735394832463731 286 NA FT VACUOLAR TRANSPORT Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters amino acid export from vacuole transporter activity|L-glutamine transmembrane transporter activity|L-isoleucine transmembrane transporter activity|L-tyrosine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YGL215W YGL215W CLG1 hom Verified 1.45737833798108 0.0725059866241893 no 473 0.0856689605878613 333 NA FT CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 positive regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YKR076W YKR076W ECM4 hom Verified 1.45583508017645 0.0727191078084616 no 474 0.077123162754114 340 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization|glutathione metabolic process glutathione transferase activity cytoplasm YHR180W_d YHR180W YHR180W hom Dubious 1.45479476078536 0.0728630445358861 no 475 0.0617411878948685 275 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR125C YJR125C ENT3 hom Verified 1.45279146661873 0.0731408309800906 no 476 0.0739185620125245 293 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|CYTOSKELETON Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p late endosome to vacuole transport via multivesicular body sorting pathway|early endosome to Golgi transport|actin filament organization|endocytosis|actin cortical patch assembly|Golgi to endosome transport protein binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding clathrin vesicle coat|actin cortical patch YLR330W YLR330W CHS5 hom Verified 1.45152231351706 0.0733172367465034 no 477 0.118572570759071 468 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM GOLGI APPARATUS|SITE OF POLARIZED GROWTH Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane ascospore wall assembly|conjugation with cellular fusion|cell wall chitin catabolic process|Golgi to plasma membrane transport|cellular bud site selection|regulation of transcription, DNA-dependent molecular_function mating projection tip|cytoplasm|exomer complex YNL243W YNL243W SLA2 hom Verified 1.45025418602724 0.0734938248387629 no 478 0.0718231678379586 283 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo bipolar cellular bud site selection|fungal-type cell wall organization|actin filament organization|endocytosis|exocytosis protein binding, bridging incipient cellular bud site|mating projection tip|actin cortical patch YLR412W YLR412W BER1 hom Verified 1.44763590745119 0.0738594516805634 no 479 0.0677712593863508 290 NA FT CYTOSKELETON Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene biological_process molecular_function cytoplasm YGR064W_d YGR064W YGR064W hom Dubious 1.44711107457398 0.0739329083977476 no 480 0.146980788697174 500 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Unknown Unknown Unknown YKR050W YKR050W TRK2 hom Verified 1.44446872586665 0.0743035853848625 no 481 0.0839377166021821 270 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication cellular potassium ion homeostasis potassium ion transmembrane transporter activity integral to membrane|plasma membrane YPL174C YPL174C NIP100 hom Verified 1.4412414024467 0.0747582476997561 no 482 0.0898249835566582 393 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION CYTOSKELETON Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) establishment of mitotic spindle orientation microtubule binding dynactin complex|cell cortex|spindle pole body|astral microtubule YOL044W YOL044W PEX15 hom Verified 1.44045533005226 0.0748693096749202 no 483 0.0604288621879542 270 NA FT PROTEIN LOCALIZATION|SIGNALING|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly protein import into peroxisome matrix, receptor recycling protein anchor integral to peroxisomal membrane YOL013W-B_d YOL013W-B YOL013W-B hom Dubious 1.43170222069819 0.0761145303030391 no 484 0.0521889848877463 270 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Unknown Unknown Unknown YLR352W_p YLR352W YLR352W hom Uncharacterized 1.4285592289495 0.0765654798046815 no 485 0.0677990510322873 283 NA Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene biological_process molecular_function SCF ubiquitin ligase complex YBR248C YBR248C HIS7 hom Verified 1.42694585732947 0.07679775070387 no 486 0.067526132769108 270 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor purine nucleoside monophosphate biosynthetic process|histidine biosynthetic process imidazoleglycerol-phosphate synthase activity intracellular YNL237W YNL237W YTP1 hom Verified 1.41821032856484 0.0780646836572029 no 487 0.138254863196502 529 NA FF|FT MITOCHONDRION Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins biological_process molecular_function integral to membrane|membrane YDR007W YDR007W TRP1 hom Verified 1.41553413473179 0.0784559737301787 no 488 0.23919449683068 894 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) tryptophan biosynthetic process phosphoribosylanthranilate isomerase activity cytoplasm YDL119C_p YDL119C YDL119C hom Uncharacterized 1.41545064339353 0.0784682049906519 no 489 0.088974943610279 430 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria transport transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YDR403W YDR403W DIT1 hom Verified 1.41511396625096 0.0785175419709633 no 490 0.0662490552890023 296 NA FT CELL WALL ORG/BIOGENESIS Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure ascospore wall assembly catalytic activity cellular_component YMR080C YMR080C NAM7 hom Verified 1.41129667083424 0.0790785778622371 no 491 0.0958759755447325 313 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION|GENE SILENCING NUCLEUS ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|intracellular mRNA localization|translational frameshifting|regulation of translational termination|chromatin silencing at silent mating-type cassette|protein ubiquitination RNA-dependent ATPase activity|ATP-dependent RNA helicase activity|nucleic acid binding mitochondrion|polysome|cytoplasm YAL021C YAL021C CCR4 hom Verified 1.4081756644569 0.0795395301876116 no 492 0.128876931588868 531 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING NUCLEUS RNA POL II, HOLOENZYME Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|DNA replication checkpoint|nuclear-transcribed mRNA poly(A) tail shortening|traversing start control point of mitotic cell cycle|replication fork protection|transcription elongation from RNA polymerase II promoter|DNA replication 3'-5'-exoribonuclease activity CCR4-NOT core complex|cytoplasmic mRNA processing body|Cdc73/Paf1 complex|cytoplasm YIL077C_p YIL077C YIL077C hom Uncharacterized 1.40784131983612 0.0795890310342956 no 493 0.0963915701665025 360 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function mitochondrion YJL162C YJL162C JJJ2 hom Verified 1.39767252585488 0.0811057141064545 no 494 0.0709599273387194 283 NA FT NUCLEUS Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein biological_process molecular_function nucleus|cytoplasm YDR512C YDR512C EMI1 hom Verified 1.3974361088562 0.0811412335276696 no 495 0.0822761949306383 342 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MITOCHONDRION ORGANIZATION Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs ascospore formation|mitochondrial respiratory chain complex assembly|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YLL044W_d YLL044W YLL044W hom Dubious 1.39656006240829 0.0812729535706564 no 496 0.0782146427750734 357 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Unknown Unknown Unknown YLL018C-A YLL018C-A COX19 hom Verified 1.39643515013901 0.0812917481844869 no 497 0.097426917535007 358 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex IV assembly|metal ion transport copper ion binding|copper chaperone activity cytosol|mitochondrial intermembrane space YER080W YER080W AIM9 hom Verified 1.39465463764167 0.081560005147466 no 498 0.0867099622499058 287 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YOR230W YOR230W WTM1 hom Verified 1.39438755838996 0.0816003015565533 no 499 0.0818895427779648 276 NA FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING NUCLEUS Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats negative regulation of chromatin silencing at silent mating-type cassette|maintenance of protein location in nucleus|negative regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of chromatin silencing at telomere|protein import into nucleus|positive regulation of transcription from RNA polymerase II promoter protein binding|transcription corepressor activity nucleus YPR031W YPR031W NTO1 hom Verified 1.39412189633685 0.0816403990318112 no 500 0.102324713888933 378 NA FT CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 histone acetylation histone acetyltransferase activity|methylated histone residue binding nucleus|NuA3 histone acetyltransferase complex|cytoplasm YOR178C YOR178C GAC1 hom Verified 1.39357588468988 0.0817228574727872 no 501 0.0836547557033869 320 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock meiosis|glycogen metabolic process|negative regulation of transcription from RNA polymerase II promoter|response to heat|mitotic cell cycle spindle assembly checkpoint protein phosphatase type 1 regulator activity|structural molecule activity|heat shock protein binding protein phosphatase type 1 complex YOR359W YOR359W VTS1 hom Verified 1.39293955129373 0.081819035467093 no 502 0.099352590890865 405 NA FT PROTEIN LOCALIZATION|RNA PROCESSING NUCLEUS Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity nuclear-transcribed mRNA catabolic process|positive regulation of endodeoxyribonuclease activity|nuclear-transcribed mRNA poly(A) tail shortening flap-structured DNA binding|RNA binding cytosol|nucleus|cytoplasmic mRNA processing body YLR124W_d YLR124W YLR124W hom Dubious 1.39240120457247 0.0819004699578999 no 503 0.084364841440762 346 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL018C YOL018C TLG2 hom Verified 1.39175428337672 0.0819984090472448 no 504 0.208351271253908 726 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT GOLGI APPARATUS Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) inositolphosphoceramide metabolic process|Golgi to vacuole transport|endocytosis|mannosyl-inositol phosphorylceramide metabolic process|protein exit from endoplasmic reticulum|vesicle fusion|CVT pathway SNAP receptor activity SNARE complex|trans-Golgi network|integral to endosome membrane YOR366W_d YOR366W YOR366W hom Dubious 1.39109323094901 0.0820985786323089 no 505 0.0886366630207697 359 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Unknown Unknown Unknown YKL072W YKL072W STB6 hom Verified 1.38934343120762 0.0823641715385004 no 506 0.0510813455759675 198 NA Protein that binds Sin3p in a two-hybrid assay biological_process molecular_function cellular_component YPL250C YPL250C ICY2 hom Verified 1.38924709211711 0.08237881311176 no 507 0.0969878040986164 374 NA Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate mitochondrion degradation molecular_function cellular_component YCR091W YCR091W KIN82 hom Verified 1.38840880313654 0.0825062986390266 no 508 0.0694089224475445 301 NA FT MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p phospholipid translocation|protein phosphorylation|response to pheromone protein kinase activity cellular_component YBR300C_d YBR300C YBR300C hom Dubious 1.38725346577658 0.0826822436583932 no 509 0.100074789868741 387 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Unknown Unknown Unknown YLR345W_p YLR345W YLR345W hom Uncharacterized 1.38583184090563 0.0828991286133444 no 510 0.0859406037628039 340 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene regulation of glycolysis|fructose 2,6-bisphosphate metabolic process 6-phosphofructo-2-kinase activity cytoplasm YKL029C YKL029C MAE1 hom Verified 1.38437164077041 0.0831223439445588 no 511 0.0743133145647563 359 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids pyruvate metabolic process|cellular amino acid metabolic process malic enzyme activity mitochondrion YCR037C YCR037C PHO87 hom Verified 1.38071087800451 0.0836839376359809 no 512 0.0847960621675586 361 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication phosphate ion transport|transmembrane transport inorganic phosphate transmembrane transporter activity integral to membrane|membrane YCR010C YCR010C ADY2 hom Verified 1.3789632598118 0.0839530409897584 no 513 0.100222146615522 382 NA FT CELL CYCLE|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication nitrogen utilization|ammonium transport|acetate transport|transmembrane transport acetate transmembrane transporter activity|ammonium transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YEL067C_p YEL067C YEL067C hom Uncharacterized 1.37710852751836 0.0842393483105015 no 514 0.0877454266521728 330 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOL080C YOL080C REX4 hom Verified 1.37705173466946 0.0842481267358074 no 515 0.0686177013740502 216 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly ribosomal large subunit assembly|rRNA processing 3'-5' exonuclease activity nucleolus|nucleus YPL008W YPL008W CHL1 hom Verified 1.37698904595268 0.0842578172778724 no 516 0.0868050095488168 390 NA FT CELL CYCLE|CHROMOSOME SEGREGATION NUCLEUS Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome negative regulation of DNA recombination|chromosome segregation|donor selection|DNA replication initiation|mitotic sister chromatid cohesion DNA helicase activity nucleus YOL154W YOL154W ZPS1 hom Verified 1.37332438155694 0.084825763089318 no 517 0.0609890343936606 248 NA Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH biological_process molecular_function fungal-type cell wall|fungal-type vacuole YER144C YER144C UBP5 hom Verified 1.37130286158817 0.0851402820134454 no 518 0.0828218243079242 338 NA FT PROTEOLYSIS SITE OF POLARIZED GROWTH Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck protein deubiquitination ubiquitin-specific protease activity incipient cellular bud site|cellular bud neck YMR252C_p YMR252C YMR252C hom Uncharacterized 1.37066778413004 0.0852392709484829 no 519 0.0682134647641028 318 NA FT MITOCHONDRION Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene biological_process molecular_function mitochondrion YER083C YER083C GET2 hom Verified 1.37058032517614 0.0852529098470982 no 520 0.162498083565952 621 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division retrograde vesicle-mediated transport, Golgi to ER|protein insertion into ER membrane protein anchor integral to membrane|endoplasmic reticulum|GET complex YDR069C YDR069C DOA4 hom Verified 1.36987167430779 0.0853634816176018 no 521 0.104485606330958 451 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|PROTEOLYSIS MITOCHONDRION Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole protein deubiquitination|endocytosis|regulation of DNA replication|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|free ubiquitin chain depolymerization|ubiquitin homeostasis|ubiquitin-dependent protein catabolic process endopeptidase activity|ubiquitin-specific protease activity|ubiquitin thiolesterase activity endosome|proteasome complex|mitochondrion YBR028C YBR028C YPK3 hom Verified 1.36452143057692 0.0862017570234189 no 522 0.110491889050586 388 NA FT PROTEIN PHOSPHORYLATION An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner biological_process protein kinase activity cytoplasm YIL009C-A YIL009C-A EST3 hom Verified 1.35955586349282 0.0869852565782623 no 523 0.0971519817660276 361 NA FT NUCLEUS|CHROMOSOME Component of the telomerase holoenzyme, involved in telomere replication telomere maintenance via telomerase ATP-dependent DNA/RNA helicase activity|GTPase activity|telomeric DNA binding chromosome, telomeric region|nucleus|telomerase holoenzyme complex YGL023C YGL023C PIB2 hom Verified 1.35716970943613 0.0873636464629301 no 524 0.0975009830656065 344 NA FT VESICLE-MEDIATED TRANSPORT MITOCHONDRION Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 biological_process molecular_function mitochondrion YKL038W YKL038W RGT1 hom Verified 1.35414268831724 0.0878454296568519 no 525 0.118378281663603 445 NA FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM NUCLEUS Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication glucose transport|regulation of glucose import|negative regulation of transcription from RNA polymerase II promoter|glucose metabolic process|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nucleus YER047C YER047C SAP1 hom Verified 1.35338068329085 0.0879670226429727 no 526 0.0997299131421468 410 NA Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system biological_process ATPase activity cytoplasm YOR115C YOR115C TRS33 hom Verified 1.351656137749 0.0882426713859801 no 527 0.0804508606433447 407 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS ENDOPLASMIC RETICULUM|GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport|protein complex assembly molecular_function trans-Golgi network|TRAPP complex|cis-Golgi network YFR046C YFR046C CNN1 hom Verified 1.35123254617247 0.0883104760034133 no 528 0.0723269705064126 313 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T chromosome segregation molecular_function nucleus|kinetochore YJL178C YJL178C ATG27 hom Verified 1.34794328774855 0.0888383119243255 no 529 0.0912422927422836 353 NA FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site vesicle organization|peroxisome degradation|protein localization to pre-autophagosomal structure|CVT pathway|autophagic vacuole assembly phosphatidylinositol-3-phosphate binding trans-Golgi network|mitochondrion|pre-autophagosomal structure|membrane YPL071C_p YPL071C YPL071C hom Uncharacterized 1.34722185258211 0.0889543959870775 no 530 0.0776033688016718 284 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YKR078W YKR078W YKR078W hom Verified 1.34694748300344 0.0889985736343375 no 531 0.0939060439342166 380 NA Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) biological_process phosphatidylinositol-3-phosphate binding cytoplasm YLR319C YLR319C BUD6 hom Verified 1.34539412135244 0.0892489963374943 no 532 0.100179688739674 304 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate bipolar cellular bud site selection|positive regulation of formin-nucleated actin cable assembly|cellular bud site selection|Rho protein signal transduction|extrachromosomal circular DNA localization involved in cell aging|establishment of cell polarity|response to osmotic stress|cytokinesis cytoskeletal regulatory protein binding polarisome|actin cap|spindle pole body|mating projection tip YJL024C YJL024C APS3 hom Verified 1.34416815097761 0.0894470090666797 no 533 0.105041063332819 435 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YLR313C YLR313C SPH1 hom Verified 1.34350163100884 0.0895547991296959 no 534 0.103909738939904 437 NA FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p bipolar cellular bud site selection|pseudohyphal growth|invasive filamentous growth|mating projection assembly molecular_function incipient cellular bud site|cellular bud neck|cellular bud tip|mating projection YER169W YER169W RPH1 hom Verified 1.3403392137862 0.0900675440799158 no 535 0.103391666109436 441 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|histone demethylation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|histone demethylase activity (H3-K36 specific)|histone demethylase activity (H3-K9 specific) nucleus YAR029W_p YAR029W YAR029W hom Uncharacterized 1.3403276127933 0.0900694290375159 no 536 0.0813811053034269 346 NA Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function cytoplasm YGR224W YGR224W AZR1 hom Verified 1.33994524444887 0.0901315735826298 no 537 0.0753778923042742 346 NA FT PLASMA MEMBRANE Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole azole transport azole transporter activity integral to membrane|plasma membrane YCR007C_p YCR007C YCR007C hom Uncharacterized 1.33791063836251 0.0904627844046548 no 538 0.105436659395754 372 NA Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene biological_process molecular_function cellular_component YOR120W YOR120W GCY1 hom Verified 1.33674771038239 0.0906525013263885 no 539 0.12582247216945 443 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication cellular response to oxidative stress|arabinose catabolic process|D-xylose catabolic process oxidoreductase activity|mRNA binding|alditol:NADP+ 1-oxidoreductase activity|aldo-keto reductase (NADP) activity nucleus|cytoplasm YNL096C YNL096C RPS7B hom Verified 1.33621011649974 0.0907403026822699 no 540 0.103303598683341 411 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS RIBOSOME Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosome biogenesis structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit YJL172W YJL172W CPS1 hom Verified 1.33471371463004 0.0909850315726273 no 541 0.107512296817037 335 NA FT PROTEOLYSIS Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions nitrogen compound metabolic process|proteolysis involved in cellular protein catabolic process carboxypeptidase activity fungal-type vacuole lumen YMR304W YMR304W UBP15 hom Verified 1.3343820593518 0.0910393383237871 no 542 0.123350788027258 383 NA FT PROTEOLYSIS Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p protein deubiquitination ubiquitin-specific protease activity peroxisome|cytoplasm YGL050W YGL050W TYW3 hom Verified 1.33208000645059 0.0914169497577721 no 543 0.0888824748778823 327 NA FT RNA PROCESSING tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions tRNA methylation|wybutosine biosynthetic process tRNA methyltransferase activity cellular_component YJR092W YJR092W BUD4 hom Verified 1.33149078761684 0.0915137871259933 no 544 0.0757633829582864 282 NA FT CELL CYCLE|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p cellular bud site selection|axial cellular bud site selection GTP binding cellular bud neck contractile ring YDR452W YDR452W PPN1 hom Verified 1.32898039901399 0.091927218337527 no 545 0.128047662660318 326 NA FT NUCLEUS Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress polyphosphate catabolic process|polyphosphate metabolic process endopolyphosphatase activity|exopolyphosphatase activity fungal-type vacuole membrane|nucleus YGL188C-A_p YGL188C-A YGL188C-A hom Uncharacterized 1.32707227820072 0.0922423872653727 no 546 0.112682270432007 477 NA Putative protein of unknown function biological_process molecular_function cellular_component YPR147C_p YPR147C YPR147C hom Uncharacterized 1.32673515608856 0.0922981535767578 no 547 0.101609929093821 394 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm YER167W YER167W BCK2 hom Verified 1.32647621003965 0.0923410050439388 no 548 0.114991675818246 457 NA FT CELL CYCLE NUCLEUS Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations regulation of cell cycle|positive regulation of gene expression|G1/S transition of mitotic cell cycle molecular_function nucleus|cytoplasm YMR141C_d YMR141C YMR141C hom Dubious 1.32582204436161 0.0924493246720865 no 549 0.0955664057466575 347 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCR101C_p YCR101C YCR101C hom Uncharacterized 1.32534224387212 0.0925288318920157 no 550 0.093461650228891 416 NA Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene biological_process molecular_function integral to membrane|membrane YLR114C YLR114C AVL9 hom Verified 1.32492767456956 0.0925975704503382 no 551 0.102534180934587 353 NA FT VESICLE-MEDIATED TRANSPORT Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress post-Golgi vesicle-mediated transport molecular_function cytoplasm YOR267C YOR267C HRK1 hom Verified 1.32301501374608 0.0929151925068312 no 552 0.0565189486397315 224 NA FT ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress protein phosphorylation|cellular ion homeostasis protein serine/threonine kinase activity|protein kinase activity cytoplasm YNL143C_p YNL143C YNL143C hom Uncharacterized 1.32209853762614 0.0930676703495396 no 553 0.110780889550062 350 NA Protein of unknown function; expressed at both mRNA and protein levels biological_process molecular_function cellular_component YIL030C YIL030C SSM4 hom Verified 1.32161300793739 0.0931485247950238 no 554 0.098225691358318 392 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM UBIQUITIN LIGASE COMPLEX Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation ER-associated protein catabolic process ubiquitin-protein ligase activity nuclear envelope|integral to endoplasmic reticulum membrane|Doa10p ubiquitin ligase complex|integral to membrane|nuclear inner membrane YGR125W_p YGR125W YGR125W hom Uncharacterized 1.32138119827734 0.0931871459729059 no 555 0.0937837651908783 409 NA Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole biological_process molecular_function integral to membrane|fungal-type vacuole YPR121W YPR121W THI22 hom Verified 1.32009027552808 0.0934024395617745 no 556 0.0781739238654221 292 NA Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis thiamine biosynthetic process phosphomethylpyrimidine kinase activity|hydroxymethylpyrimidine kinase activity cellular_component YOL013W-A_p YOL013W-A YOL013W-A hom Uncharacterized 1.31988951675464 0.0934359540845407 no 557 0.0902756056373567 350 NA Putative protein of unknown function; identified by SAGE biological_process molecular_function integral to membrane YPR119W YPR119W CLB2 hom Verified 1.31982683227334 0.0934464204055211 no 558 0.109823674337997 486 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation cyclin-dependent protein kinase regulator activity nucleus|spindle pole body|cellular bud neck|spindle|cytoplasm YOR003W YOR003W YSP3 hom Verified 1.31896582790679 0.0935902684833603 no 559 0.0776222365717881 332 NA FT PROTEOLYSIS Putative precursor to the subtilisin-like protease III protein catabolic process peptidase activity cellular_component YHR125W_d YHR125W YHR125W hom Dubious 1.31584493575787 0.0941130467868858 no 560 0.106115607126476 461 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YMR222C YMR222C FSH2 hom Verified 1.31428262528419 0.0943755560173166 no 561 0.0845100422207619 329 NA Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process molecular_function cytoplasm YKL113C YKL113C RAD27 hom Verified 1.3106959745838 0.0949802496069873 no 562 0.128112648228849 494 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION 5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family double-strand break repair via nonhomologous end joining|replicative cell aging|DNA replication, removal of RNA primer|base-excision repair, base-free sugar-phosphate removal|gene conversion at mating-type locus, DNA repair synthesis 5'-3' exonuclease activity|5'-flap endonuclease activity mitochondrion|nucleus YOR306C YOR306C MCH5 hom Verified 1.31050986819084 0.0950117040414738 no 563 0.0751537050329101 290 NA FT PLASMA MEMBRANE Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport riboflavin transport monocarboxylic acid transmembrane transporter activity|riboflavin transporter activity integral to membrane|plasma membrane YMR316W YMR316W DIA1 hom Verified 1.31039092184165 0.0950318115618397 no 564 0.109908429075261 425 NA Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern pseudohyphal growth|invasive growth in response to glucose limitation molecular_function cytoplasm YHR193C YHR193C EGD2 hom Verified 1.30832339883396 0.0953818209797643 no 565 0.0942435715339707 371 NA FT PROTEIN LOCALIZATION NUCLEUS Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes 'de novo' cotranslational protein folding unfolded protein binding|phosphatidic acid binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding nascent polypeptide-associated complex YDR112W_d YDR112W IRC2 hom Dubious 1.30741398158091 0.0955360756977568 no 566 0.089754343653974 364 NA Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Unknown Unknown Unknown YBL098W YBL098W BNA4 hom Verified 1.30700986277641 0.095604680934704 no 567 0.0871336595651492 429 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease de novo NAD biosynthetic process from tryptophan kynurenine 3-monooxygenase activity mitochondrial outer membrane|mitochondrion YNL133C YNL133C FYV6 hom Verified 1.30558492894237 0.0958468741317891 no 568 0.134393605456775 491 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining double-strand break repair via nonhomologous end joining molecular_function nucleus YDR199W_d YDR199W YDR199W hom Dubious 1.30483621793519 0.0959743117828326 no 569 0.116684419532113 418 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Unknown Unknown Unknown YOR220W YOR220W RCN2 hom Verified 1.30425578524189 0.0960731925932713 no 570 0.0890830061747639 342 NA Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress biological_process molecular_function cytoplasm YBR294W YBR294W SUL1 hom Verified 1.30407509568859 0.0961039896185425 no 571 0.0687462938358762 298 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates sulfate transport|transmembrane transport sulfate transmembrane transporter activity integral to membrane|plasma membrane YPR201W YPR201W ARR3 hom Verified 1.30317384625409 0.0962577084510824 no 572 0.0834768533531438 359 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite arsenite transport|antimonite transport antiporter activity|arsenite transmembrane transporter activity|antimonite transmembrane transporter activity integral to membrane|plasma membrane YCL050C YCL050C APA1 hom Verified 1.30281557734756 0.0963188656573544 no 573 0.094036830213282 347 NA FT NUCLEOTIDE METABOLISM NUCLEUS AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication nucleotide biosynthetic process|nucleoside catabolic process bis(5'-nucleosyl)-tetraphosphatase activity|sulfate adenylyltransferase (ADP) activity nucleus|cytoplasm YOR166C YOR166C SWT1 hom Verified 1.29910689542973 0.0969536235207176 no 574 0.0783816972014617 312 NA FT NUCLEUS RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain nuclear mRNA surveillance of mRNP export|transcription, DNA-dependent endoribonuclease activity nucleus|cytoplasm YPR093C YPR093C ASR1 hom Verified 1.29526571813577 0.0976142905101781 no 575 0.0931537600845307 354 NA FT NUCLEUS Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein response to ethanol|protein ubiquitination ubiquitin-protein ligase activity nucleus|cytoplasm YPR120C YPR120C CLB5 hom Verified 1.28871253268504 0.0987490219136182 no 576 0.116508766719462 446 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase spindle assembly|G1/S transition of mitotic cell cycle|regulation of S phase of mitotic cell cycle|G2/M transition of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation|premeiotic DNA replication|positive regulation of DNA replication cyclin-dependent protein kinase regulator activity nucleus YHL016C YHL016C DUR3 hom Verified 1.28804629634246 0.0988649239357563 no 577 0.0789727827400224 292 NA FT PLASMA MEMBRANE Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway urea transport|spermidine transport|putrescine transport|urea catabolic process urea transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YDR435C YDR435C PPM1 hom Verified 1.28556590997517 0.0992973002391055 no 578 0.136636614265023 538 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits cellular protein complex assembly|C-terminal protein methylation protein C-terminal leucine carboxyl O-methyltransferase activity cellular_component YDR289C YDR289C RTT103 hom Verified 1.28408716443905 0.0995557293198978 no 579 0.119784555194706 483 NA FT RNA PROCESSING NUCLEUS|CHROMOSOME Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated|mRNA 3'-end processing RNA polymerase II core binding nuclear chromatin|site of double-strand break YDL093W YDL093W PMT5 hom Verified 1.2813473942115 0.100035836416342 no 580 0.105053586178831 360 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YPL095C YPL095C EEB1 hom Verified 1.28010824378123 0.100253534882271 no 581 0.116051674105762 445 NA FT KETONE METABOLISM|LIPID METABOLISM Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication medium-chain fatty acid catabolic process|medium-chain fatty acid biosynthetic process short-chain carboxylesterase activity|alcohol O-octanoyltransferase activity cellular_component YPR045C YPR045C THP3 hom Verified 1.27919606677723 0.100414010292608 no 582 0.100788355780481 379 NA FT RNA PROCESSING NUCLEUS|CHROMOSOME Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes mRNA splicing, via spliceosome molecular_function nucleus|transcriptionally active chromatin YIL146C YIL146C ATG32 hom Verified 1.27850990304688 0.100534847624185 no 583 0.0824745393574586 328 NA FT CELL WALL ORG/BIOGENESIS MITOCHONDRION Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane fungal-type cell wall organization|mitochondrion degradation molecular_function integral to mitochondrial outer membrane YDL073W_p YDL073W YDL073W hom Uncharacterized 1.2756861724497 0.101033239254326 no 584 0.129942400120891 502 NA Putative protein of unknown function; YDL073W is not an essential gene biological_process molecular_function cellular_component YDR150W YDR150W NUM1 hom Verified 1.27551703074441 0.101063150023869 no 585 0.148032260620759 556 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION MITOCHONDRION|SITE OF POLARIZED GROWTH Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex mitochondrion inheritance|nuclear migration along microtubule|mitochondrial fission|microtubule cytoskeleton organization tubulin binding cell cortex|mitochondrion|cellular bud tip YLR006C YLR006C SSK1 hom Verified 1.27424386669407 0.101288501632866 no 586 0.0940972795542241 360 NA FT SIGNALING|PROTEIN PHOSPHORYLATION Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate activation of MAPKKK activity involved in osmosensory signaling pathway|positive regulation of protein autophosphorylation|osmosensory signaling pathway via two-component system protein kinase activator activity|two-component response regulator activity|protein homodimerization activity|mitogen-activated protein kinase kinase kinase binding cytoplasm YHR159W_p YHR159W TDA11 hom Uncharacterized 1.27359951088638 0.101402692841599 no 587 0.122448143486147 438 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function cytoplasm YGR243W YGR243W FMP43 hom Verified 1.27351958312906 0.101416863984407 no 588 0.100863582774421 451 NA FT MITOCHONDRION Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|mitochondrial membrane YDL065C YDL065C PEX19 hom Verified 1.27323744646879 0.101466898176438 no 589 0.107289573674717 410 NA FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning protein stabilization|protein import into peroxisome membrane|peroxisome inheritance|ER-dependent peroxisome organization|protein exit from endoplasmic reticulum peroxisome membrane targeting sequence binding|protein binding cytosol|peroxisomal membrane|endoplasmic reticulum YMR030W YMR030W RSF1 hom Verified 1.27295607943055 0.101516813786854 no 590 0.0856590356505184 295 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS|MITOCHONDRION Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes transcription from mitochondrial promoter|calcium-mediated signaling|transcription from RNA polymerase II promoter|aerobic respiration molecular_function mitochondrion|nucleus YOL037C_d YOL037C YOL037C hom Dubious 1.27205671346093 0.101676484756802 no 591 0.110930793930299 406 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Unknown Unknown Unknown YIL012W_d YIL012W YIL012W hom Dubious 1.27062276797961 0.101931441565527 no 592 0.10294151632205 413 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YNL111C YNL111C CYB5 hom Verified 1.27029864893287 0.101989134640017 no 593 0.152449805049369 556 NA FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation sterol biosynthetic process electron carrier activity endoplasmic reticulum membrane YML129C YML129C COX14 hom Verified 1.2694806095763 0.102134851015291 no 594 0.110848457061505 396 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes mitochondrial respiratory chain complex IV assembly|negative regulation of mitochondrial translation molecular_function mitochondrial envelope|mitochondrion|integral to membrane|mitochondrial inner membrane|mitochondrial matrix|extrinsic to membrane YPR092W_d YPR092W YPR092W hom Dubious 1.2678637937358 0.10242329788803 no 595 0.112501526865729 396 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL072C YBL072C RPS8A hom Verified 1.26727470105106 0.102528541640081 no 596 0.0961428218584663 434 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YML036W YML036W CGI121 hom Verified 1.26722358705708 0.102537677063908 no 597 0.123624721927827 435 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS|CHROMOSOME Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification telomere maintenance|positive regulation of transcription from RNA polymerase II promoter molecular_function EKC/KEOPS complex YLR315W YLR315W NKP2 hom Verified 1.2664070284412 0.102683697950549 no 598 0.13841079766539 453 NA FT CELL CYCLE|CELL DIVISION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 biological_process molecular_function spindle pole body|kinetochore YAL008W YAL008W FUN14 hom Verified 1.26627109815315 0.102708020316149 no 599 0.12177794916109 410 NA FT MITOCHONDRION Mitochondrial protein of unknown function biological_process molecular_function mitochondrial outer membrane|mitochondrion YPL219W YPL219W PCL8 hom Verified 1.26560312865541 0.102827602707465 no 600 0.120075269347679 422 NA FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation negative regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YKL096W-A YKL096W-A CWP2 hom Verified 1.2606333291876 0.103720492373735 no 601 0.104493384958576 406 NA FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored fungal-type cell wall organization|regulation of pH structural constituent of cell wall fungal-type cell wall YOL162W_p YOL162W YOL162W hom Uncharacterized 1.26050575150938 0.103743487196748 no 602 0.0830239330998964 314 NA Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family transmembrane transport transmembrane transporter activity integral to membrane|membrane YPL254W YPL254W HFI1 hom Verified 1.25928607034844 0.103963511368097 no 603 0.120231171374807 465 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions histone acetylation|chromatin modification|transcription from RNA polymerase II promoter transcription coactivator activity|transcription cofactor activity SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YMR247C YMR247C RKR1 hom Verified 1.25462559761546 0.104807353284463 no 604 0.0878371284135665 333 NA FT CHROMATIN ORGANIZATION|PROTEOLYSIS|GENE SILENCING NUCLEUS RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice chromatin modification|chromatin silencing at telomere|ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity|ribosome binding ribosome|nucleus YML090W_d YML090W YML090W hom Dubious 1.25024155367483 0.105605660769609 no 605 0.100256898076522 366 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Unknown Unknown Unknown YOR147W YOR147W MDM32 hom Verified 1.24819864103466 0.105979160757368 no 606 0.105853970466131 396 NA FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 mitochondrion inheritance|cellular ion homeostasis|mitochondrion organization molecular_function mitochondrion|mitochondrial inner membrane YPR089W_p YPR089W YPR089W hom Uncharacterized 1.2471749574188 0.106166676556817 no 607 0.111846046781551 469 NA FT GOLGI APPARATUS Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p biological_process molecular_function cellular_component YBR220C_p YBR220C YBR220C hom Uncharacterized 1.24683185206129 0.106229579332332 no 608 0.101951279968511 379 NA Putative protein of unknown function; YBR220C is not an essential gene biological_process molecular_function integral to membrane YBL099W YBL099W ATP1 hom Verified 1.24571733772233 0.10643409310701 no 609 0.11921687029869 489 NA FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS MITOCHONDRION Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism mitochondrial nucleoid|proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, catalytic core YLR418C YLR418C CDC73 hom Verified 1.24514747092334 0.10653877364164 no 610 0.204849047347548 721 NA FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS|CHROMOSOME RNA POL II, HOLOENZYME Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress transcription elongation from RNA polymerase I promoter|negative regulation of DNA recombination|mRNA 3'-end processing|recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex|positive regulation of transcription elongation from RNA polymerase II promoter|Unknown|regulation of transcription-coupled nucleotide-excision repair|transcription elongation from RNA polymerase II promoter chromatin binding|TFIIF-class binding transcription factor activity|RNA polymerase II core binding|RNA polymerase II transcription factor binding transcription factor activity nucleus|transcriptionally active chromatin|Cdc73/Paf1 complex YIL167W YIL167W SDL1 hom pseudogene 1.24486411254574 0.106590852246353 no 611 0.108206603123126 419 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase serine family amino acid metabolic process L-serine ammonia-lyase activity cellular_component YDL185W YDL185W VMA1 hom Verified 1.24401359615679 0.106747279510118 no 612 0.184834301616876 648 NA FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease intron homing|cellular protein metabolic process|vacuolar acidification endodeoxyribonuclease activity|proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V1 domain|fungal-type vacuole membrane YPR173C YPR173C VPS4 hom Verified 1.24326201294123 0.106885648771805 no 613 0.142007561429729 530 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT ENDOPLASMIC RETICULUM AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism late endosome to vacuole transport via multivesicular body sorting pathway|protein retention in Golgi apparatus|intralumenal vesicle formation|protein homooligomerization|sterol metabolic process|late endosome to vacuole transport ATPase activity|protein homodimerization activity|ATP binding endosome|endoplasmic reticulum|membrane|cytoplasm YPR129W YPR129W SCD6 hom Verified 1.24267463873374 0.106993876592181 no 614 0.132517473312304 518 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress stress granule assembly|negative regulation of translational initiation|cytoplasmic mRNA processing body assembly mRNA binding|RNA binding|eukaryotic initiation factor 4G binding cytoplasmic mRNA processing body|cytoplasmic stress granule|cytoplasm YOR365C_p YOR365C YOR365C hom Uncharacterized 1.24101775113652 0.107299595697193 no 615 0.0897037373419938 386 NA Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication biological_process molecular_function integral to membrane YCL001W-B_p YCL001W-B YCL001W-B hom Uncharacterized 1.24042304671071 0.107409480485662 no 616 0.1185505817841 389 NA Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication biological_process molecular_function cellular_component YCL029C YCL029C BIK1 hom Verified 1.23819501264958 0.107821880188184 no 617 0.114062099099366 448 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON KINETOCHORE Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 karyogamy involved in conjugation with cellular fusion|mitotic anaphase B|negative regulation of microtubule polymerization|nuclear migration involved in conjugation with cellular fusion|mitotic spindle organization in nucleus|mitotic spindle elongation microtubule binding|protein homodimerization activity spindle microtubule|microtubule plus end|spindle pole body|cell cortex|condensed nuclear chromosome kinetochore|mating projection tip|kinetochore|spindle YLR410W YLR410W VIP1 hom Verified 1.23401048497016 0.108599498627032 no 618 0.0897497605305796 341 NA FT CYTOSKELETON Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 inositol phosphate biosynthetic process inositol hexakisphosphate 1-kinase activity|inositol hexakisphosphate 3-kinase activity|inositol heptakisphosphate kinase activity|inositol hexakisphosphate 4-kinase activity|inositol hexakisphosphate 6-kinase activity cytoplasm YIR039C YIR039C YPS6 hom Verified 1.23288264714145 0.108809775253258 no 619 0.127793107661271 485 NA FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS PLASMA MEMBRANE Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance fungal-type cell wall organization aspartic-type endopeptidase activity fungal-type cell wall YHR096C YHR096C HXT5 hom Verified 1.23164641173941 0.109040597896892 no 620 0.121737873918517 436 NA FT PLASMA MEMBRANE Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication glucose transport|hexose transport mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YNL010W_p YNL010W YNL010W hom Uncharacterized 1.23107992009619 0.109146487405547 no 621 0.121878098169928 452 NA FT NUCLEUS Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation biological_process molecular_function nucleus|cytoplasm YDR019C YDR019C GCV1 hom Verified 1.22683820174867 0.10994170469664 no 622 0.122500177644985 443 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm glycine catabolic process|glycine metabolic process|one-carbon metabolic process glycine dehydrogenase (decarboxylating) activity mitochondrion|glycine cleavage complex YPL038W YPL038W MET31 hom Verified 1.22671248976103 0.109965335854056 no 623 0.113950377561413 450 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication regulation of sulfur amino acid metabolic process|regulation of transcription from RNA polymerase II promoter core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YPR042C YPR042C PUF2 hom Verified 1.22667048642586 0.109973232392465 no 624 0.122955868362968 497 NA Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay mRNA binding cytoplasm YGR287C YGR287C IMA1 hom Verified 1.22475364055908 0.110334028445581 no 625 0.109070362472256 421 NA FT CARBOHYDRATE METABOLISM MITOCHONDRION Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family disaccharide catabolic process oligo-1,6-glucosidase activity mitochondrion|ribosome YCR090C_p YCR090C YCR090C hom Uncharacterized 1.22326301017099 0.11061518670494 no 626 0.0759036028378754 349 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene biological_process molecular_function nucleus|cytoplasm YML128C YML128C MSC1 hom Verified 1.22223935209965 0.110808563211175 no 627 0.121618550946701 445 NA FT CELL CYCLE ENDOPLASMIC RETICULUM|MITOCHONDRION Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated reciprocal meiotic recombination molecular_function mitochondrion|endoplasmic reticulum|plasma membrane YGR085C YGR085C RPL11B hom Verified 1.22042528551096 0.11115184839998 no 628 0.134627201534092 450 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YOL056W YOL056W GPM3 hom Verified 1.21808017032944 0.111596754286404 no 629 0.106397187217191 414 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process phosphoglycerate mutase activity|molecular_function cytosol YHR142W YHR142W CHS7 hom Verified 1.21683463988018 0.111833568757731 no 630 0.243753788194705 813 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress protein folding|ER to Golgi vesicle-mediated transport|fungal-type cell wall chitin biosynthetic process unfolded protein binding integral to membrane|endoplasmic reticulum membrane YOL029C_p YOL029C YOL029C hom Uncharacterized 1.21382801914449 0.11240670220056 no 631 0.104194713680299 422 NA Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens biological_process molecular_function cellular_component YFR026C YFR026C ULI1 hom Verified 1.21187097513028 0.112780887096119 no 632 0.112369508112174 386 NA FT SIGNALING Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response endoplasmic reticulum unfolded protein response molecular_function cellular_component YNL321W YNL321W VNX1 hom Verified 1.21117836819403 0.112913525692323 no 633 0.126244133627867 448 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate potassium ion transport|sodium ion transport|hydrogen transport potassium:hydrogen antiporter activity|sodium:hydrogen antiporter activity|calcium:hydrogen antiporter activity fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum membrane|fungal-type vacuole YDR360W_d YDR360W OPI7 hom Dubious 1.21087222460921 0.112972189592999 no 634 0.115021712091928 422 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Unknown Unknown Unknown YLR425W YLR425W TUS1 hom Verified 1.2091830089341 0.113296271961721 no 635 0.162857258501845 610 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS SITE OF POLARIZED GROWTH Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate fungal-type cell wall organization|signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|cellular bud neck YBR078W YBR078W ECM33 hom Verified 1.20769775187534 0.113581771545837 no 636 0.192915312800787 643 NA FF|FT CELL WALL ORG/BIOGENESIS MITOCHONDRION|PLASMA MEMBRANE GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p fungal-type cell wall organization molecular_function mitochondrion|fungal-type cell wall|plasma membrane YDR011W YDR011W SNQ2 hom Verified 1.20668667364013 0.113776416580955 no 637 0.154254473854521 584 NA FF|FT NUCLEOTIDE METABOLISM MITOCHONDRION|PLASMA MEMBRANE Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species response to drug xenobiotic-transporting ATPase activity mitochondrion|integral to membrane|plasma membrane YDL172C_d YDL172C YDL172C hom Dubious 1.20497687725897 0.114106114208358 no 638 0.136300616739177 510 NA Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL214W YJL214W HXT8 hom Verified 1.20352153115499 0.114387282236932 no 639 0.112629256423745 395 NA FT PLASMA MEMBRANE Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose hexose transport mannose transmembrane transporter activity|glucose transmembrane transporter activity|fructose transmembrane transporter activity integral to membrane|plasma membrane YLR372W YLR372W SUR4 hom Verified 1.2011063046376 0.114854983868965 no 640 0.279980157093596 952 NA FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM ENDOPLASMIC RETICULUM Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis post-Golgi vesicle-mediated transport|fatty acid elongation|fatty acid biosynthetic process|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum YBL067C YBL067C UBP13 hom Verified 1.19700026744496 0.115653225253477 no 641 0.12731172582226 485 NA FT PROTEOLYSIS Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication biological_process ubiquitin-specific protease activity cellular_component YMR225C YMR225C MRPL44 hom Verified 1.19477379668207 0.116087710353623 no 642 0.112189834096229 438 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR432W YLR432W IMD3 hom Verified 1.19073996202906 0.116877842334623 no 643 0.146669134722355 526 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed GTP biosynthetic process IMP dehydrogenase activity plasma membrane|cytoplasm YIR024C YIR024C YIR024C hom Verified 1.1896267951107 0.117096554721029 no 644 0.129833828917824 448 NA FT MITOCHONDRION Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect biological_process molecular_function mitochondrion YCL010C YCL010C SGF29 hom Verified 1.18922912365066 0.117174758519592 no 645 0.103419046000089 415 NA FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation histone H3-K14 acetylation|cellular protein complex localization|histone H3-K18 acetylation|cellular protein localization|histone H3-K9 acetylation|positive regulation of transcription from RNA polymerase II promoter methylated histone residue binding|RNA polymerase II transcription factor recruiting transcription factor activity Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YDR300C YDR300C PRO1 hom Verified 1.18917237564152 0.117185921275035 no 646 0.121218551454707 421 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication proline biosynthetic process glutamate 5-kinase activity cytoplasm YPL100W YPL100W ATG21 hom Verified 1.18680888494767 0.117651506706667 no 647 0.122936085304363 448 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein piecemeal microautophagy of nucleus|vesicle organization|mitochondrion degradation|macroautophagy|protein localization to pre-autophagosomal structure|CVT pathway|protein lipidation phosphatidylinositol binding endosome|cytosol|fungal-type vacuole membrane YOR360C YOR360C PDE2 hom Verified 1.18391867405981 0.118222628630053 no 648 0.137195385379545 466 NA FT SIGNALING NUCLEUS High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon cAMP-mediated signaling 3',5'-cyclic-AMP phosphodiesterase activity nucleus|cytoplasm YBR242W_p YBR242W YBR242W hom Uncharacterized 1.18380142847331 0.118245838321386 no 649 0.158076009597691 523 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene biological_process molecular_function nucleus|cytoplasm YFR035C_p YFR035C YFR035C hom Uncharacterized 1.1836437212297 0.118277062798991 no 650 0.110482724878607 443 NA Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein biological_process molecular_function cellular_component YKR043C YKR043C SHB17 hom Verified 1.18258495103725 0.118486839793812 no 651 0.157473849140446 533 NA FT CARBOHYDRATE METABOLISM NUCLEUS Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus ribose phosphate biosynthetic process sedoheptulose-bisphosphatase activity nucleus|cytoplasm YCR036W YCR036W RBK1 hom Verified 1.18244456209336 0.118514675167136 no 652 0.169049762531333 630 NA FT CARBOHYDRATE METABOLISM NUCLEUS Putative ribokinase D-ribose metabolic process ribokinase activity|ATP binding nucleus|cytoplasm YLL032C YLL032C YLL032C hom Verified 1.18243321234658 0.118516925720306 no 653 0.0983561779216192 330 NA Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene biological_process molecular_function ribosome|cytoplasm YOR346W YOR346W REV1 hom Verified 1.18106631189588 0.118788190735375 no 654 0.0987656734823163 352 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION|CHROMOSOME Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress error-prone translesion synthesis|error-free translesion synthesis deoxycytidyl transferase activity|DNA-directed DNA polymerase activity nuclear chromatin|mitochondrion|replication fork YMR040W YMR040W YET2 hom Verified 1.18019412128272 0.118961508312611 no 655 0.128167384792037 353 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein biological_process molecular_function ribosome|integral to membrane YNL196C_p YNL196C SLZ1 hom Uncharacterized 1.17752672077604 0.119492669388855 no 656 0.12916543744795 515 NA Sporulation-specific protein with a leucine zipper motif biological_process molecular_function cellular_component YLR434C_d YLR434C YLR434C hom Dubious 1.17708724359624 0.119580343038793 no 657 0.137490476819672 471 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Unknown Unknown Unknown YBR176W YBR176W ECM31 hom Verified 1.17670850359692 0.11965593629826 no 658 0.126296135233072 376 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM MITOCHONDRION Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate pantothenate biosynthetic process 3-methyl-2-oxobutanoate hydroxymethyltransferase activity mitochondrion YER152C YER152C YER152C hom Verified 1.17622751030572 0.119751987010759 no 659 0.102916563146461 368 NA FT NUCLEUS Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene biological_process 2-aminoadipate transaminase activity nucleus|cytoplasm YDL213C YDL213C NOP6 hom Verified 1.17215232866093 0.120567951915741 no 660 0.127537514372192 469 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes ribosomal small subunit biogenesis RNA binding|rRNA binding nucleolus|90S preribosome YDL233W_p YDL233W MFG1 hom Uncharacterized 1.17212064448296 0.120574311270594 no 661 0.109245015513284 419 NA FT NUCLEUS Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene biological_process molecular_function nucleus|cytoplasm YOR158W YOR158W PET123 hom Verified 1.16987419264025 0.121025800428653 no 662 0.133202060827762 478 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YKR055W YKR055W RHO4 hom Verified 1.16912200926147 0.121177238795151 no 663 0.112812749188605 480 NA FT SIGNALING|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity positive regulation of formin-nucleated actin cable assembly|maintenance of cell polarity|regulation of formin-nucleated actin cable assembly GTPase activity incipient cellular bud site|plasma membrane|cellular bud neck YNR031C YNR031C SSK2 hom Verified 1.16827239321582 0.121348453686164 no 664 0.118632270958694 472 NA FT SIGNALING|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress MAPK cascade involved in osmosensory signaling pathway|protein autophosphorylation|protein phosphorylation|response to osmotic stress|actin cytoskeleton organization|osmosensory signaling pathway via two-component system MAP kinase kinase kinase activity cytosol|cellular bud neck|cellular bud tip YJR056C YJR056C YJR056C hom Verified 1.16753560191925 0.121497069756842 no 665 0.0751270662673542 305 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YDR194W-A_p YDR194W-A YDR194W-A hom Uncharacterized 1.16679137387681 0.121647315714348 no 666 0.144673414483175 503 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YDR432W YDR432W NPL3 hom Verified 1.16502133981286 0.122005177474783 no 667 0.151080589619568 571 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION NUCLEUS RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress translational termination|mRNA export from nucleus|negative regulation of translation|mRNA splicing, via spliceosome|Unknown|positive regulation of transcription elongation from RNA polymerase II promoter mRNA binding|RNA polymerase II core binding|eukaryotic initiation factor 4G binding|poly(A) RNA binding nucleus|cytoplasm YGR228W_d YGR228W YGR228W hom Dubious 1.16302209274959 0.122410269461177 no 668 0.154640115894046 577 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Unknown Unknown Unknown YER024W YER024W YAT2 hom Verified 1.16276384906618 0.122462664142535 no 669 0.116002661801939 372 NA FT KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane carnitine metabolic process|alcohol metabolic process carnitine O-acetyltransferase activity cytosol|cytoplasm YEL015W YEL015W EDC3 hom Verified 1.15974902416081 0.123075501988725 no 670 0.133720212103207 469 NA FT PROTEIN COMPLEX ORG/BIOGENESIS Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress deadenylation-independent decapping of nuclear-transcribed mRNA|cytoplasmic mRNA processing body assembly molecular_function cytoplasmic mRNA processing body YGR144W YGR144W THI4 hom Verified 1.15103962521122 0.124857967071419 no 671 0.145672568885264 491 NA FT MITOCHONDRION ORGANIZATION NUCLEUS Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents thiamine biosynthetic process|mitochondrial genome maintenance|thiazole biosynthetic process ferrous iron binding cytosol YDL034W_d YDL034W YDL034W hom Dubious 1.1496101360837 0.125152240756141 no 672 0.116077042032079 430 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Unknown Unknown Unknown YLR325C YLR325C RPL38 hom Verified 1.14953906031214 0.125166884996396 no 673 0.147511553384473 490 NA FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YAL042C-A_d YAL042C-A YAL042C-A hom Dubious 1.14941280434845 0.125192901349653 no 674 0.125738736182979 462 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Unknown Unknown Unknown YER178W YER178W PDA1 hom Verified 1.14779364577906 0.125526880547465 no 675 0.144613415616824 510 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose acetyl-CoA biosynthetic process from pyruvate pyruvate dehydrogenase (acetyl-transferring) activity mitochondrial nucleoid|mitochondrion|mitochondrial pyruvate dehydrogenase complex YGL159W_p YGL159W YGL159W hom Uncharacterized 1.14745605258242 0.125596593189662 no 676 0.14167788397392 533 NA Putative protein of unknown function; deletion mutant has no detectable phenotype biological_process molecular_function cellular_component YBL032W YBL032W HEK2 hom Verified 1.14633943954614 0.125827365042172 no 677 0.117645404001825 467 NA FT CHROMATIN ORGANIZATION|TRANSLATION|RNA LOCALIZATION NUCLEUS|CHROMOSOME RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K telomere maintenance via telomerase|intracellular mRNA localization|mRNA stabilization mRNA binding nuclear chromosome, telomeric region|cytoplasmic mRNA processing body|cytoplasm YKR041W YKR041W YKR041W hom Verified 1.14536680260061 0.126028622037521 no 678 0.104509453657205 378 NA FT NUCLEUS|CYTOSKELETON Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking biological_process molecular_function nucleus|mitotic spindle|cytoplasm YOR173W YOR173W DCS2 hom Verified 1.14290164739474 0.126539714251076 no 679 0.124487860972558 474 NA Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance deadenylation-dependent decapping of nuclear-transcribed mRNA m7G(5')pppN diphosphatase activity cytoplasmic mRNA processing body|cytoplasm YLR358C_p YLR358C YLR358C hom Uncharacterized 1.14211000299145 0.126704149028165 no 680 0.10988704315441 481 NA Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W biological_process molecular_function cellular_component YOR307C YOR307C SLY41 hom Verified 1.14089753002783 0.126956283707208 no 681 0.135435257212022 455 NA FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM Protein involved in ER-to-Golgi transport ER to Golgi vesicle-mediated transport molecular_function integral to membrane|endoplasmic reticulum YPL198W YPL198W RPL7B hom Verified 1.14056939859296 0.127024578894103 no 682 0.134073197906865 477 NA FT TRANSLATION NUCLEUS RIBOSOME Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|nucleolus|cytoplasm YBR249C YBR249C ARO4 hom Verified 1.13961402756314 0.127223569317317 no 683 0.123254103969914 469 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress aromatic amino acid family biosynthetic process 3-deoxy-7-phosphoheptulonate synthase activity nucleus|cytoplasm YPL183C YPL183C RTT10 hom Verified 1.13952418547093 0.127242293318582 no 684 0.132930210899448 468 NA FT VESICLE-MEDIATED TRANSPORT WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 endocytic recycling molecular_function endosome|cytoplasm YER011W YER011W TIR1 hom Verified 1.13761100757953 0.127641474092589 no 685 0.104472441794871 400 NA Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking response to stress structural constituent of cell wall fungal-type cell wall YER114C YER114C BOI2 hom Verified 1.13626924344134 0.127921949378099 no 686 0.158926610201319 541 NA FT SIGNALING|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication cytokinesis, completion of separation|establishment of cell polarity|budding cell apical bud growth|Rho protein signal transduction phospholipid binding site of polarized growth|cellular bud|cellular bud neck YJR147W YJR147W HMS2 hom Verified 1.13380056728549 0.128439106499629 no 687 0.103243116212041 332 NA FT NUCLEUS Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication pseudohyphal growth molecular_function nucleus|cytoplasm YNL014W YNL014W HEF3 hom Verified 1.13349456484153 0.128503311182801 no 688 0.1405463650619 477 NA FT TRANSLATION|NUCLEOTIDE METABOLISM RIBOSOME Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication translational elongation ATPase activity|translation elongation factor activity cytosolic ribosome YOL007C YOL007C CSI2 hom Verified 1.13285603839496 0.128637356969582 no 689 0.121725616166202 460 NA FT SITE OF POLARIZED GROWTH Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene biological_process molecular_function fungal-type vacuole|cellular bud neck YMR295C YMR295C YMR295C hom Verified 1.13168136502811 0.128884209392213 no 690 0.120817056858672 427 NA FT SITE OF POLARIZED GROWTH Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; YMR295C is not an essential gene; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|cellular bud|mating projection tip YPL222W_p YPL222W FMP40 hom Uncharacterized 1.13140716729922 0.128941878102227 no 691 0.129465753158768 470 NA FT MITOCHONDRION Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YGR106C YGR106C VOA1 hom Verified 1.13130647866122 0.128963059223938 no 692 0.194976122034593 667 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval protein complex assembly molecular_function fungal-type vacuole membrane|endoplasmic reticulum membrane YGL149W_d YGL149W YGL149W hom Dubious 1.13097023783608 0.129033809194474 no 693 0.119512258205113 422 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Unknown Unknown Unknown YER149C YER149C PEA2 hom Verified 1.1277412145887 0.129714613144029 no 694 0.117163609127574 438 NA FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth bipolar cellular bud site selection|regulation of initiation of mating projection growth|regulation of termination of mating projection growth|actin filament organization|pseudohyphal growth|establishment of cell polarity|filamentous growth|Rho protein signal transduction cytoskeletal regulatory protein binding polarisome|actin cap|mating projection tip YBL078C YBL078C ATG8 hom Verified 1.12513422262399 0.130266080644552 no 695 0.165186485281094 607 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS ENDOMEMBRANE SYSTEM Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis piecemeal microautophagy of nucleus|mitochondrion degradation|cellular membrane fusion|ER to Golgi vesicle-mediated transport|CVT pathway|autophagic vacuole assembly molecular_function cytosol|fungal-type vacuole membrane|pre-autophagosomal structure|extrinsic to membrane|autophagic vacuole YMR286W YMR286W MRPL33 hom Verified 1.12493227867897 0.130308866249743 no 696 0.132281516322549 526 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR402W_d YLR402W YLR402W hom Dubious 1.12446373233326 0.130408174007041 no 697 0.143525372040061 457 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKR005C_p YKR005C YKR005C hom Uncharacterized 1.12435864204982 0.130430454934262 no 698 0.122012839778819 476 NA Putative protein of unknown function biological_process molecular_function cellular_component YGR171C YGR171C MSM1 hom Verified 1.12432342051136 0.130437923088458 no 699 0.139937163670668 502 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p methionyl-tRNA aminoacylation methionine-tRNA ligase activity mitochondrion YNR052C YNR052C POP2 hom Verified 1.1240439339344 0.130497194154619 no 700 0.207756273254898 723 NA FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS|SITE OF POLARIZED GROWTH RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter 3'-5'-exoribonuclease activity CCR4-NOT core complex|mating projection tip|cytoplasmic mRNA processing body|cytoplasm YOR118W YOR118W RTC5 hom Verified 1.1236391100665 0.130583078679818 no 701 0.156155110170854 545 NA Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity biological_process molecular_function cytoplasm YCR069W YCR069W CPR4 hom Verified 1.12161016028859 0.131014114873687 no 702 0.119585589907103 437 NA Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway biological_process peptidyl-prolyl cis-trans isomerase activity membrane YOR386W YOR386W PHR1 hom Verified 1.11953456429268 0.131456076880741 no 703 0.145602738358652 535 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p photoreactive repair mRNA binding|deoxyribodipyrimidine photo-lyase activity mitochondrion|nucleus YBR268W YBR268W MRPL37 hom Verified 1.11844036108738 0.131689482248926 no 704 0.177886876714825 629 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YLR121C YLR121C YPS3 hom Verified 1.118270608215 0.131725717979325 no 705 0.134313068347844 490 NA FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS PLASMA MEMBRANE Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor fungal-type cell wall organization|cellular protein metabolic process aspartic-type endopeptidase activity anchored to plasma membrane|plasma membrane YDL021W YDL021W GPM2 hom Verified 1.11627279023728 0.132152692497889 no 706 0.138203319010542 483 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event biological_process phosphoglycerate mutase activity|molecular_function cytosol|cytoplasm YIL059C_d YIL059C YIL059C hom Dubious 1.11600385521916 0.132210242194989 no 707 0.102720518601066 376 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Unknown Unknown Unknown YKR101W YKR101W SIR1 hom Verified 1.11416570604453 0.132604052340144 no 708 0.116200318525197 437 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin establishment of chromatin silencing|chromatin silencing at silent mating-type cassette chromatin binding|sequence-specific DNA binding chromatin silencing complex YAR050W YAR050W FLO1 hom Verified 1.1130721510114 0.132838721592532 no 709 0.153212686690438 492 NA FT PLASMA MEMBRANE Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p cellular response to stress|flocculation via cell wall protein-carbohydrate interaction|flocculation mannose binding fungal-type cell wall YML123C YML123C PHO84 hom Verified 1.11219185324058 0.133027834969733 no 710 0.177962840130777 625 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity phosphate ion transport|manganese ion transport|polyphosphate metabolic process manganese ion transmembrane transporter activity|inorganic phosphate transmembrane transporter activity integral to membrane|integral to plasma membrane YJR111C_p YJR111C YJR111C hom Uncharacterized 1.11121551465409 0.133237797350266 no 711 0.128225802321773 515 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria biological_process molecular_function mitochondrion YER119C YER119C AVT6 hom Verified 1.10845896912 0.133831824927809 no 712 0.118880213637148 459 NA FT VACUOLAR TRANSPORT Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication amino acid export from vacuole|transmembrane transport L-aspartate transmembrane transporter activity|transporter activity|L-glutamate transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YER068W YER068W MOT2 hom Verified 1.10679998655935 0.134190207029686 no 713 0.148413586638996 492 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|protein polyubiquitination|protein ubiquitination ubiquitin-protein ligase activity|sequence-specific DNA binding CCR4-NOT core complex|cytoplasm YIL020C YIL020C HIS6 hom Verified 1.10631396558509 0.134295324561865 no 714 0.136265723389107 485 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts histidine biosynthetic process 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity intracellular YMR106C YMR106C YKU80 hom Verified 1.10590368718726 0.13438410435318 no 715 0.118624959802788 470 NA FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair chromatin assembly or disassembly|protein localization to chromosome|chromatin silencing|double-strand break repair via nonhomologous end joining|donor selection|telomere maintenance|double-strand break repair via homologous recombination damaged DNA binding|RNA binding|DNA binding|telomeric DNA binding nuclear chromatin|nuclear telomeric heterochromatin|Ku70:Ku80 complex YMR031W-A_d YMR031W-A YMR031W-A hom Dubious 1.10581960390712 0.134402304036889 no 716 0.132459400457574 471 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Unknown Unknown Unknown YBL107C YBL107C MIC23 hom Verified 1.10487199849126 0.134607528637593 no 717 0.145133084278767 508 NA Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) biological_process molecular_function cytoplasm YNL297C YNL297C MON2 hom Verified 1.10477673081898 0.134628172820103 no 718 0.164916123862663 502 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins transport|endocytosis|protein targeting to vacuole|Golgi to endosome transport guanyl-nucleotide exchange factor activity cytosol|endosome|trans-Golgi network|extrinsic to membrane YGR049W YGR049W SCM4 hom Verified 1.10400638727324 0.134795183488507 no 719 0.143078377560292 437 NA FT MITOCHONDRION Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to membrane YJL205C YJL205C NCE101 hom Verified 1.10105807314477 0.135435691538562 no 720 0.11845979815466 457 NA FT PROTEIN LOCALIZATION Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences protein secretion molecular_function cellular_component YMR201C YMR201C RAD14 hom Verified 1.09859512026085 0.135972353561713 no 721 0.143825451411747 520 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein nucleotide-excision repair, DNA damage recognition damaged DNA binding|zinc ion binding nucleotide-excision repair factor 1 complex|nucleus YCR051W_p YCR051W YCR051W hom Uncharacterized 1.09842664420597 0.136009116565955 no 722 0.13547893540244 520 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene biological_process molecular_function nucleus|cytoplasm YHR018C YHR018C ARG4 hom Verified 1.09803818239388 0.136093908389736 no 723 0.125815693988946 447 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway arginine biosynthetic process via ornithine argininosuccinate lyase activity cytosol YFR040W YFR040W SAP155 hom Verified 1.09671315605066 0.136383401767477 no 724 0.147081933041276 559 NA FT CELL CYCLE Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity cytoplasm YNL012W YNL012W SPO1 hom Verified 1.09661187387938 0.136405547338608 no 725 0.115110042792652 437 NA FT CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B meiosis|spore membrane bending pathway phospholipase activity endoplasmic reticulum|nucleus|prospore membrane YLR118C YLR118C YLR118C hom Verified 1.09488604743523 0.136783281213162 no 726 0.114965164055545 379 NA FT KETONE METABOLISM|LIPID METABOLISM NUCLEUS Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS protein deacylation palmitoyl-(protein) hydrolase activity nucleus|cytoplasm YPL108W_p YPL108W YPL108W hom Uncharacterized 1.09322493675486 0.137147525413128 no 727 0.149610124864233 518 NA Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cytoplasm YBR269C_p YBR269C FMP21 hom Uncharacterized 1.09261505714261 0.137281424440746 no 728 0.139298042702683 491 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDL182W YDL182W LYS20 hom Verified 1.08715072995371 0.138485100601199 no 729 0.110896289591299 399 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS|MITOCHONDRION Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication lysine biosynthetic process via aminoadipic acid homocitrate synthase activity mitochondrion|nucleus YLR001C_p YLR001C YLR001C hom Uncharacterized 1.08683289320504 0.138555333984974 no 730 0.123871973783558 471 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated biological_process molecular_function mitochondrion|fungal-type vacuole membrane YBR077C YBR077C SLM4 hom Verified 1.08561873225161 0.138823854351387 no 731 0.169358048336339 564 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|SIGNALING Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 signal transduction|microautophagy phosphatidylinositol-3,4-bisphosphate binding fungal-type vacuole membrane|EGO complex|late endosome membrane|cytoplasm YDR363W-A YDR363W-A SEM1 hom Verified 1.08311180560804 0.139379399750158 no 732 0.145259751149385 550 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|PROTEOLYSIS|RNA LOCALIZATION NUCLEUS Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|regulation of cell cycle|mRNA export from nucleus|exocytosis|filamentous growth|ubiquitin-dependent protein catabolic process molecular_function cytosol|nucleus|proteasome storage granule|proteasome regulatory particle, lid subcomplex YLL042C YLL042C ATG10 hom Verified 1.08217502076455 0.13958738282931 no 733 0.114287435863893 393 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy piecemeal microautophagy of nucleus|cellular protein modification process|mitochondrion degradation|macroautophagy|CVT pathway Atg12 ligase activity cellular_component YDR316W YDR316W OMS1 hom Verified 1.0820700795123 0.139610694813034 no 734 0.165787098967432 598 NA FT MITOCHONDRION Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations biological_process S-adenosylmethionine-dependent methyltransferase activity mitochondrial envelope|mitochondrion YPR115W YPR115W RGC1 hom Verified 1.08200236109206 0.13962573940374 no 735 0.146338945858627 512 NA FT CELL CYCLE Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication positive regulation of glycerol transport molecular_function cytoplasm YIR001C YIR001C SGN1 hom Verified 1.07910272699157 0.140270967867242 no 736 0.136663855059098 484 NA Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation mRNA metabolic process poly(A) RNA binding cytoplasm YOL113W YOL113W SKM1 hom Verified 1.07818337016913 0.14047596589921 no 737 0.154936325893819 588 NA FT CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS|PLASMA MEMBRANE Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth negative regulation of gene expression|cytokinetic cell separation|protein phosphorylation|sterol import protein serine/threonine kinase activity|protein kinase activity plasma membrane|nucleus YFR007W YFR007W YFH7 hom Verified 1.07783783635052 0.140553065535158 no 738 0.126155937399723 463 NA Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases phosphorylation ATPase activity|kinase activity cellular_component YKR026C YKR026C GCN3 hom Verified 1.07723961840113 0.140686614977796 no 739 0.18860973608781 723 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YIL090W YIL090W ICE2 hom Verified 1.07711651110886 0.140714108803106 no 740 0.151205646596722 520 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells endoplasmic reticulum inheritance molecular_function integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum YPR188C YPR188C MLC2 hom Verified 1.07697250255428 0.140746275180823 no 741 0.135168351145546 461 NA FT CELL CYCLE|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring actomyosin contractile ring contraction myosin II binding cellular bud neck contractile ring YNL273W YNL273W TOF1 hom Verified 1.07408686339741 0.141391875861283 no 742 0.130990063733238 444 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase DNA repair|DNA replication checkpoint|maintenance of DNA repeat elements|replication fork arrest|replication fork protection|DNA replication|mitotic sister chromatid cohesion molecular_function nuclear chromosome|replication fork protection complex|nuclear replication fork YKL214C YKL214C YRA2 hom Verified 1.07349254534562 0.141525090802248 no 743 0.11711038663686 443 NA FT RNA LOCALIZATION NUCLEUS Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus poly(A)+ mRNA export from nucleus RNA binding nucleus YMR084W_p YMR084W YMR084W hom Uncharacterized 1.07288095861156 0.141662265235329 no 744 0.146315967797941 530 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 biological_process molecular_function cellular_component YCR034W YCR034W FEN1 hom Verified 1.07028514694203 0.142245488993643 no 745 0.228128070384603 793 NA FF|FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication fatty acid elongation|vesicle-mediated transport|sphingolipid biosynthetic process fatty acid elongase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum membrane YJR146W_p YJR146W YJR146W hom Uncharacterized 1.06491407540638 0.143457407443291 no 746 0.128038662657925 450 NA Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 biological_process molecular_function cellular_component YBR214W YBR214W SDS24 hom Verified 1.06324897933174 0.143834527970386 no 747 0.143088855635421 492 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication endocytosis|cytokinetic cell separation molecular_function cytoplasm YGL181W YGL181W GTS1 hom Verified 1.06215588042244 0.144082462625374 no 748 0.147493318242324 470 NA FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|MEMBRANE ORGANIZATION|SIGNALING|NUCLEOTIDE METABOLISM NUCLEUS|CYTOSKELETON Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations endocytosis|ultradian rhythm|regulation of transcription from RNA polymerase II promoter|negative regulation of phosphatidylinositol biosynthetic process|positive regulation of transcription from RNA polymerase II promoter ARF GTPase activator activity|repressing transcription factor binding|transcription factor binding transcription factor activity nucleus|cytoplasm|actin cortical patch YLR263W YLR263W RED1 hom Verified 1.0612019515939 0.144299066338396 no 749 0.117773559406863 422 NA FT CELL CYCLE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p synaptonemal complex assembly|meiotic recombination checkpoint|positive regulation of catalytic activity chromatin DNA binding|structural molecule activity lateral element YER111C YER111C SWI4 hom Verified 1.06117854707327 0.1443043834397 no 750 0.145524900094754 564 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS|CHROMOSOME DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription involved in G1/S phase of mitotic cell cycle sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription SBF transcription complex|nuclear chromatin YOR277C_d YOR277C YOR277C hom Dubious 1.060925074698 0.144361976428511 no 751 0.165547790983712 576 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Unknown Unknown Unknown YBL039W-B_p YBL039W-B YBL039W-B hom Uncharacterized 1.05978164448859 0.144621974660027 no 752 0.128949589593525 474 NA Putative protein of unknown function biological_process molecular_function cellular_component YGR063C YGR063C SPT4 hom Verified 1.05770911423272 0.145094039375347 no 753 0.207292730132276 705 NA FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|CHROMOSOME SEGREGATION|GENE SILENCING NUCLEUS|CHROMOSOME Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions 7-methylguanosine mRNA capping|chromosome segregation|chromatin organization|intracellular mRNA localization|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription, DNA-dependent|regulation of transcription-coupled nucleotide-excision repair|chromatin silencing|mRNA splicing, via spliceosome|Unknown core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rDNA binding DSIF complex|nuclear telomeric heterochromatin|rDNA heterochromatin|mating-type region heterochromatin|kinetochore YPL189W YPL189W GUP2 hom Verified 1.05728826437374 0.145190023793684 no 754 0.0961641356053997 353 NA Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog glycerol transport O-acyltransferase activity integral to membrane|membrane YJL149W YJL149W DAS1 hom Verified 1.05698702894527 0.145258753642034 no 755 0.159176356931808 592 NA FT PROTEOLYSIS UBIQUITIN LIGASE COMPLEX Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YOR314W_d YOR314W YOR314W hom Dubious 1.0568552590805 0.145288825123614 no 756 0.139746949162282 527 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLL006W-A_p YLL006W-A YLL006W-A hom Uncharacterized 1.05645348534831 0.145380540615994 no 757 0.115337229447382 426 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YBR064W_d YBR064W YBR064W hom Dubious 1.05589507302032 0.145508077682856 no 758 0.127449877584202 457 NA Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Unknown Unknown Unknown YPL202C YPL202C AFT2 hom Verified 1.05374200333676 0.146000526375073 no 759 0.122363954115008 484 NA FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication cellular response to oxidative stress|cellular iron ion homeostasis|positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription mitochondrion|nucleus YJR110W YJR110W YMR1 hom Verified 1.05321181527895 0.146121962261348 no 760 0.146061846713745 527 NA FT LIPID METABOLISM Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family phosphatidylinositol dephosphorylation mRNA binding|phosphatidylinositol-3-phosphatase activity cytoplasm YJL160C_p YJL160C YJL160C hom Uncharacterized 1.05219937846472 0.146354042304539 no 761 0.179602283346705 605 NA Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication biological_process molecular_function cellular_component YGR121C YGR121C MEP1 hom Verified 1.05214484199861 0.146366550673945 no 762 0.134940651784504 452 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YHR067W YHR067W HTD2 hom Verified 1.05068347992289 0.146701992925678 no 763 0.180466756155376 646 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology fatty acid biosynthetic process 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity mitochondrion YLR099C YLR099C ICT1 hom Verified 1.05054604943536 0.146733565347711 no 764 0.150222792092636 569 NA FT KETONE METABOLISM|LIPID METABOLISM Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication phosphatidic acid biosynthetic process lysophosphatidic acid acyltransferase activity cellular_component YGL168W YGL168W HUR1 hom Verified 1.04853013832015 0.147197212083642 no 765 0.217420404949955 739 NA FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene DNA replication molecular_function cellular_component YDL107W YDL107W MSS2 hom Verified 1.04828381640042 0.147253931816319 no 766 0.148155172637523 532 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p protein insertion into mitochondrial membrane from inner side molecular_function extrinsic to mitochondrial inner membrane|mitochondrial matrix YDR492W YDR492W IZH1 hom Verified 1.04746013712526 0.147443704101567 no 767 0.142549581245381 555 NA FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc response to zinc ion|cellular zinc ion homeostasis|cellular lipid metabolic process metal ion binding integral to membrane|endoplasmic reticulum|membrane YHR017W YHR017W YSC83 hom Verified 1.04458206473988 0.148108086221584 no 768 0.177275288212873 666 NA FT MITOCHONDRION Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 biological_process molecular_function mitochondrial outer membrane|mitochondrion YOL109W YOL109W ZEO1 hom Verified 1.04434079044266 0.148163873512336 no 769 0.154798657159681 523 NA FT CELL WALL ORG/BIOGENESIS MITOCHONDRION|PLASMA MEMBRANE Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria fungal-type cell wall organization|response to stress molecular_function mitochondrial outer membrane|mitochondrion|extrinsic to plasma membrane|plasma membrane YNR049C YNR049C MSO1 hom Verified 1.04233748664617 0.148627619145491 no 770 0.146870084268101 507 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress cellular membrane fusion|ascospore-type prospore membrane assembly|vesicle docking involved in exocytosis molecular_function SNARE complex|cellular bud membrane|plasma membrane|prospore membrane|cellular bud neck|cellular bud tip YOL046C_d YOL046C YOL046C hom Dubious 1.04071262614748 0.149004470754041 no 771 0.126232470965981 455 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Unknown Unknown Unknown YBR085W YBR085W AAC3 hom Verified 1.03787925344657 0.149663136713876 no 772 0.132189450354013 485 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication heme biosynthetic process|heme transport|anaerobic respiration|transmembrane transport ATP:ADP antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YMR158W YMR158W MRPS8 hom Verified 1.03739704068692 0.149775428468034 no 773 0.14769426105473 525 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YFR034C YFR034C PHO4 hom Verified 1.03703582427464 0.149859580895071 no 774 0.187442846192677 634 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS NUCLEUS Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability cellular response to phosphate starvation|positive regulation of phosphate metabolic process|phosphate-containing compound metabolic process|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus|cytoplasm YMR034C_p YMR034C YMR034C hom Uncharacterized 1.03601641146437 0.150097243095087 no 775 0.159553591577717 465 NA Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene biological_process sterol transporter activity integral to membrane YNR073C_p YNR073C YNR073C hom Uncharacterized 1.03589773114778 0.150124928113159 no 776 0.179658108889189 549 NA Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p biological_process mannitol dehydrogenase activity cellular_component YOL057W YOL057W YOL057W hom Verified 1.03548238391647 0.150221844575264 no 777 0.135179734135752 448 NA FT PROTEOLYSIS NUCLEUS Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers proteolysis dipeptidyl-peptidase activity nucleus|cytoplasm YPL111W YPL111W CAR1 hom Verified 1.03464878069548 0.150416482030459 no 778 0.161519476557954 589 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS|SITE OF POLARIZED GROWTH Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance regulation of ornithine metabolic process|arginine catabolic process to ornithine manganese ion binding|arginase activity|ornithine carbamoyltransferase inhibitor activity|zinc ion binding cytosol|nucleus|mating projection tip|cytoplasm YLR417W YLR417W VPS36 hom Verified 1.03446285029419 0.150459917701473 no 779 0.208591212310343 746 NA FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome negative regulation of transcription from RNA polymerase II promoter by glucose|protein retention in Golgi apparatus|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein targeting to vacuole ubiquitin binding ESCRT II complex YHR031C YHR031C RRM3 hom Verified 1.0310819808015 0.151251188508188 no 780 0.172478810421793 584 NA FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION NUCLEUS|CHROMOSOME DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p mitochondrial genome maintenance|replication fork progression beyond termination site|DNA replication ATP-dependent DNA helicase activity|DNA helicase activity nuclear telomeric heterochromatin|replication fork YER163C YER163C GCG1 hom Verified 1.03056722206778 0.151371906852674 no 781 0.148552895465619 569 NA FT NUCLEUS Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle biological_process molecular_function nucleus|cytoplasm YNL047C YNL047C SLM2 hom Verified 1.02991956762035 0.151523882156913 no 782 0.124434173166817 453 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication eisosome assembly|TOR signaling cascade|regulation of cell growth|actin filament bundle assembly|endosomal transport|establishment or maintenance of actin cytoskeleton polarity|actin cytoskeleton organization phosphatidylinositol binding plasma membrane YPL185W_d YPL185W YPL185W hom Dubious 1.0297907236691 0.151554128117712 no 783 0.158962107336563 505 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Unknown Unknown Unknown YLR251W YLR251W SYM1 hom Verified 1.02941440387575 0.15164249171095 no 784 0.13861946890674 462 NA FT MITOCHONDRION Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 ethanol metabolic process molecular_function mitochondrion|integral to membrane|mitochondrial inner membrane YPL200W YPL200W CSM4 hom Verified 1.02888364669081 0.151767176921722 no 785 0.152504387022693 541 NA FT CELL CYCLE|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CHROMOSOME Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements meiotic telomere clustering|meiotic chromosome segregation|synapsis|regulation of reciprocal meiotic recombination molecular_function nuclear chromosome, telomeric region|endoplasmic reticulum membrane YPL092W YPL092W SSU1 hom Verified 1.02778462521618 0.152025575021084 no 786 0.154254533719543 463 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein sulfite transport sulfite transmembrane transporter activity integral to membrane|plasma membrane YDR138W YDR138W HPR1 hom Verified 1.02737973908378 0.15212084404971 no 787 0.2178110098831 724 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION NUCLEUS RNA POL II, HOLOENZYME Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|DNA recombination|response to DNA damage stimulus|transcription elongation from RNA polymerase II promoter protein complex scaffold|nucleic acid binding THO complex part of transcription export complex|Cdc73/Paf1 complex|nucleoplasmic THO complex YDR209C_d YDR209C YDR209C hom Dubious 1.02591999587299 0.152464648250854 no 788 0.138128239093557 484 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Unknown Unknown Unknown YLR309C YLR309C IMH1 hom Verified 1.02543138791002 0.15257984212961 no 789 0.134514549265373 489 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Golgi to plasma membrane protein transport|vesicle-mediated transport molecular_function cytosol|Golgi apparatus YLL016W YLL016W SDC25 hom pseudogene 1.02417641351533 0.152875978601382 no 790 0.146132304830305 518 NA FT SIGNALING Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Ras protein signal transduction Ras guanyl-nucleotide exchange factor activity cellular_component YDL223C YDL223C HBT1 hom Verified 1.02401322808189 0.152914513471492 no 791 0.183885065112771 637 NA Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis cell morphogenesis involved in conjugation with cellular fusion molecular_function plasma membrane|mating projection YFR032C_p YFR032C RRT5 hom Uncharacterized 1.02387621841794 0.152946872126625 no 792 0.148108821069744 576 NA Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation biological_process molecular_function cellular_component YLR183C YLR183C TOS4 hom Verified 1.02301578137048 0.15315019212118 no 793 0.157644391076645 533 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION|CHROMOSOME Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication response to DNA damage stimulus chromatin binding nuclear chromatin|Rpd3L-Expanded complex|mitochondrion|nucleus|cytoplasm YPR172W_p YPR172W YPR172W hom Uncharacterized 1.02094833744832 0.153639458025284 no 794 0.147708401482579 571 NA FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM NUCLEUS Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YDR214W YDR214W AHA1 hom Verified 1.01971463886941 0.153931908462741 no 795 0.164994772606333 567 NA FT NUCLEOTIDE METABOLISM Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress protein folding|response to stress ATPase activator activity|chaperone binding cytoplasm YDR179C YDR179C CSN9 hom Verified 1.01952029544416 0.153978011480292 no 796 0.14945103116009 487 NA FT SIGNALING NUCLEUS Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation molecular_function signalosome YDL137W YDL137W ARF2 hom Verified 1.01420365321666 0.155242792895911 no 797 0.161602916518523 568 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS GOLGI APPARATUS ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport GTPase activity cytosol|Golgi-associated vesicle YGR110W YGR110W CLD1 hom Verified 1.01412397103661 0.155261800579956 no 798 0.168935383544317 515 NA FT LIPID METABOLISM MITOCHONDRION Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 cardiolipin acyl-chain remodeling|cardiolipin metabolic process phospholipase A2 activity mitochondrion YLR329W YLR329W REC102 hom Verified 1.01167448189137 0.155846860237917 no 799 0.172372001762178 581 NA FT CELL CYCLE NUCLEUS|CHROMOSOME Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome|nucleus YGL143C YGL143C MRF1 hom Verified 1.01122296913336 0.155954862355504 no 800 0.208714111175351 695 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability translational termination|mitochondrial translational termination|mitochondrial translation translation release factor activity mitochondrion YJR154W_p YJR154W YJR154W hom Uncharacterized 1.01041632653259 0.156147934511334 no 801 0.148001249593327 524 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YPL137C YPL137C GIP3 hom Verified 1.00609082466789 0.15718594097846 no 802 0.158918026902504 537 NA FT CHROMOSOME SEGREGATION ENDOPLASMIC RETICULUM|MITOCHONDRION Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication chromosome segregation|regulation of phosphoprotein phosphatase activity protein phosphatase type 1 regulator activity|protein phosphatase 1 binding mitochondrion|ribosome|endoplasmic reticulum|cytoplasm YPR036W YPR036W VMA13 hom Verified 1.00447035438481 0.157575976813807 no 803 0.21721894265036 728 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V1 domain|fungal-type vacuole membrane|integral to membrane YMR120C YMR120C ADE17 hom Verified 1.0038899225673 0.157715837234626 no 804 0.135390218727509 521 NA FT NUCLEOTIDE METABOLISM Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol|plasma membrane YAL066W_d YAL066W YAL066W hom Dubious 1.00386747920577 0.157721246807367 no 805 0.181036245595438 547 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR180C YOR180C DCI1 hom Verified 1.00323039924253 0.157874854424302 no 806 0.184660770738806 636 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed fatty acid beta-oxidation dodecenoyl-CoA delta-isomerase activity peroxisomal matrix YDR451C YDR451C YHP1 hom Verified 1.00281931781383 0.157974023214679 no 807 0.216812273058147 765 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS|CHROMOSOME Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription involved in G1 phase of mitotic cell cycle RNA polymerase II regulatory region sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription nuclear chromatin|nucleus YHR093W_d YHR093W AHT1 hom Dubious 1.00250686673457 0.158049425888715 no 808 0.151788298565313 518 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Unknown Unknown Unknown YJR038C_d YJR038C YJR038C hom Dubious 1.00237386087991 0.158081530872384 no 809 0.14760715635151 505 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDL133W YDL133W SRF1 hom Verified 1.00088699007873 0.158440723484857 no 810 0.215606620272219 671 NA Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients positive regulation of catalytic activity molecular_function integral to membrane YOR137C YOR137C SIA1 hom Verified 1.00071101906476 0.158483269297127 no 811 0.139864050041429 530 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose proton transport molecular_function cellular_component YGL216W YGL216W KIP3 hom Verified 0.999317486242624 0.158820458637829 no 812 0.129571117618108 464 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Kinesin-related motor protein involved in mitotic spindle positioning nuclear migration along microtubule|mitotic spindle disassembly|establishment of mitotic spindle orientation|plus-end specific microtubule depolymerization|mitotic spindle organization in nucleus plus-end-directed microtubule motor activity|tubulin-dependent ATPase activity kinesin complex|cytoplasmic microtubule|nuclear microtubule YBL100W-C_p YBL100W-C YBL100W-C hom Uncharacterized 0.999206041832451 0.15884744482998 no 813 0.15550483964464 551 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YOR177C YOR177C MPC54 hom Verified 0.997572316659112 0.159243395272599 no 814 0.168748537503402 579 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION NUCLEUS|CYTOSKELETON Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate ascospore wall assembly|vesicle docking structural molecule activity spindle pole body YJL129C YJL129C TRK1 hom Verified 0.997333208130404 0.159301399912408 no 815 0.206562744082433 706 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication cellular potassium ion homeostasis potassium ion transmembrane transporter activity membrane raft|integral to membrane|plasma membrane YGR109C YGR109C CLB6 hom Verified 0.997031484096625 0.159374614007274 no 816 0.1494408232275 527 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 regulation of cyclin-dependent protein kinase activity|G1/S transition of mitotic cell cycle|regulation of S phase of mitotic cell cycle|premeiotic DNA replication cyclin-dependent protein kinase regulator activity cellular_component YOR106W YOR106W VAM3 hom Verified 0.996720238730886 0.159450161559116 no 817 0.195929508758998 693 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region autophagic vacuole fusion|vesicle fusion with vacuole|piecemeal microautophagy of nucleus|vacuole fusion, non-autophagic|vesicle docking|vesicle fusion protein homodimerization activity|SNAP receptor activity SNARE complex|fungal-type vacuole membrane YPR038W_d YPR038W IRC16 hom Dubious 0.994725254660731 0.159934954035509 no 818 0.151377209791719 554 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Unknown Unknown Unknown YEL066W YEL066W HPA3 hom Verified 0.990376219419363 0.160995132237635 no 819 0.110831921646888 404 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro D-amino acid metabolic process D-amino-acid N-acetyltransferase activity nucleus|cytoplasm YDL174C YDL174C DLD1 hom Verified 0.989680755501168 0.161165092115133 no 820 0.132631941202966 476 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane cellular carbohydrate metabolic process|aerobic respiration D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial inner membrane YHL012W_p YHL012W YHL012W hom Uncharacterized 0.985820216042636 0.162110671787544 no 821 0.130417275322647 463 NA Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication biological_process molecular_function cellular_component YBR264C YBR264C YPT10 hom Verified 0.984611163219419 0.162407552087995 no 822 0.160855286842248 548 NA FT PROTEIN LOCALIZATION|SIGNALING PLASMA MEMBRANE Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Golgi organization GTPase activity|guanyl nucleotide binding cytoplasm YLR258W YLR258W GSY2 hom Verified 0.984047204865078 0.162546151785473 no 823 0.162438062681921 574 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress glycogen biosynthetic process glycogen (starch) synthase activity cytoplasm YML019W YML019W OST6 hom Verified 0.98403063929955 0.162550224140008 no 824 0.175135999488531 630 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p protein N-linked glycosylation via asparagine|protein complex assembly dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YLL019C YLL019C KNS1 hom Verified 0.982699531646402 0.162877670650575 no 825 0.135392884868929 472 NA FT PROTEIN PHOSPHORYLATION Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation negative regulation of transcription from RNA polymerase III promoter|protein autophosphorylation|protein phosphorylation protein serine/threonine kinase activity|protein tyrosine kinase activity|protein kinase activity nucleus|cytoplasm YGL070C YGL070C RPB9 hom Verified 0.981584344766672 0.163152331578228 no 826 0.201696920822827 668 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE NUCLEUS RNA POL II, HOLOENZYME RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription transcription initiation from RNA polymerase II promoter|maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter|transcription-coupled nucleotide-excision repair|response to DNA damage stimulus|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity DNA-directed RNA polymerase II, core complex YDR502C YDR502C SAM2 hom Verified 0.981455841541951 0.163184000145469 no 827 0.1356730952917 476 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication methionine metabolic process|S-adenosylmethionine biosynthetic process methionine adenosyltransferase activity cellular_component YEL012W YEL012W UBC8 hom Verified 0.978306108880434 0.163961474723457 no 828 0.161395499614137 570 NA FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity cytoplasm YEL052W YEL052W AFG1 hom Verified 0.976186997229213 0.164485902801195 no 829 0.149082764420275 517 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS|RESPONSE TO OXIDATIVE STRESS|PEROXISOME ORGANIZATION MITOCHONDRION Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain protein import into peroxisome matrix|cellular response to oxidative stress|misfolded or incompletely synthesized protein catabolic process molecular_function mitochondrion|mitochondrial inner membrane YDR525W_d YDR525W API2 hom Dubious 0.976087592995095 0.164510529573761 no 830 0.210029697527677 698 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Unknown Unknown Unknown YDL178W YDL178W DLD2 hom Verified 0.975772773258606 0.164588539947565 no 831 0.207393882824139 700 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION D-lactate dehydrogenase, located in the mitochondrial matrix lactate metabolic process actin binding|D-lactate dehydrogenase (cytochrome) activity mitochondrion|mitochondrial matrix YOR108W YOR108W LEU9 hom Verified 0.975368032019096 0.164688867525267 no 832 0.153236927110075 546 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication leucine biosynthetic process 2-isopropylmalate synthase activity mitochondrion YML094W YML094W GIM5 hom Verified 0.974686465448468 0.16485790430568 no 833 0.163303795648094 603 NA FT PROTEIN COMPLEX ORG/BIOGENESIS Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YKL085W YKL085W MDH1 hom Verified 0.973268272957084 0.165209993293875 no 834 0.171161923754891 587 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated chronological cell aging|replicative cell aging|tricarboxylic acid cycle|aerobic respiration L-malate dehydrogenase activity|mRNA binding mitochondrion|mitochondrial matrix YHL042W_p YHL042W YHL042W hom Uncharacterized 0.973098116260947 0.165252270093192 no 835 0.148458690021268 491 NA Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins biological_process molecular_function cellular_component YJL095W YJL095W BCK1 hom Verified 0.972932740460895 0.165293365748886 no 836 0.258985382527385 938 NA FF|FT SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION SITE OF POLARIZED GROWTH Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p intracellular protein kinase cascade|endoplasmic reticulum unfolded protein response|peroxisome degradation|establishment of cell polarity|protein phosphorylation|response to acid|response to nutrient protein kinase activity|MAP kinase kinase kinase activity intracellular|mating projection tip YER059W YER059W PCL6 hom Verified 0.972774271373751 0.165332751300363 no 837 0.147568447108934 528 NA FT CELL CYCLE|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding regulation of glycogen catabolic process|regulation of glycogen biosynthetic process cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex YPR039W_d YPR039W YPR039W hom Dubious 0.972192079887818 0.165477499996661 no 838 0.188780931560358 674 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Unknown Unknown Unknown YDR315C YDR315C IPK1 hom Verified 0.971586896636801 0.165628051943094 no 839 0.217880642290248 693 NA FT NUCLEUS Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable nuclear-transcribed mRNA catabolic process, non-stop decay|inositol phosphate biosynthetic process inositol pentakisphosphate 2-kinase activity nucleus YLR387C YLR387C REH1 hom Verified 0.970433803708218 0.165915152930911 no 840 0.199389788162286 664 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS RIBOSOME Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains ribosomal large subunit biogenesis|budding cell bud growth molecular_function cytosolic large ribosomal subunit|preribosome, large subunit precursor|cytoplasm YGL085W_p YGL085W LCL3 hom Uncharacterized 0.968671116733734 0.166354653972184 no 841 0.151752335666586 566 NA FT MITOCHONDRION Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS biological_process molecular_function mitochondrion YJL183W YJL183W MNN11 hom Verified 0.968237925322326 0.166462779092247 no 842 0.199944648513709 685 NA FT CARBOHYDRATE METABOLISM GOLGI APPARATUS Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p protein glycosylation alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YJL135W_d YJL135W YJL135W hom Dubious 0.966937882253045 0.166787543805084 no 843 0.143839506155736 466 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Unknown Unknown Unknown YGL071W YGL071W AFT1 hom Verified 0.963139239224237 0.167738826137399 no 844 0.280039587180384 970 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION NUCLEUS Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation|meiotic chromosome segregation|positive regulation of iron ion transport|establishment of mitotic sister chromatid cohesion|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding nucleus|cytoplasm YCL025C YCL025C AGP1 hom Verified 0.962007701598244 0.168022867632678 no 845 0.120727298444684 423 NA FT PLASMA MEMBRANE Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication amino acid transport|transmembrane transport amino acid transmembrane transporter activity|L-proline transmembrane transporter activity integral to membrane|plasma membrane YGL118C_d YGL118C YGL118C hom Dubious 0.959978970935344 0.168532899365373 no 846 0.167600988168941 634 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL017C YPL017C IRC15 hom Verified 0.959192274876605 0.168730945726891 no 847 0.200784728106891 693 NA FT PROTEIN LOCALIZATION|CELL CYCLE|OXIDATION-REDUCTION PROCESS|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION CYTOSKELETON Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci microtubule nucleation|mitotic recombination|microtubule anchoring|meiotic sister chromatid segregation|attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation|positive regulation of mitotic cell cycle microtubule binding|S-adenosylmethionine-dependent methyltransferase activity microtubule|cytoplasm YER052C YER052C HOM3 hom Verified 0.957230732551778 0.169225404192065 no 848 0.172481142401806 627 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis threonine biosynthetic process|methionine biosynthetic process|homoserine biosynthetic process aspartate kinase activity cytoplasm YLR220W YLR220W CCC1 hom Verified 0.957185401743274 0.169236842004256 no 849 0.159490275871616 566 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress manganese ion transport|cellular calcium ion homeostasis|cellular iron ion homeostasis|cellular manganese ion homeostasis|ferrous iron transport manganese ion transmembrane transporter activity|ferrous iron transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|Golgi apparatus YBR027C_d YBR027C YBR027C hom Dubious 0.955881352886006 0.169566090449608 no 850 0.141545138242723 525 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL091C YBL091C MAP2 hom Verified 0.954579706281455 0.169895141905407 no 851 0.168601205603391 584 NA FT PROTEOLYSIS Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p protein initiator methionine removal involved in protein maturation metalloaminopeptidase activity cytoplasm YGR054W YGR054W YGR054W hom Verified 0.952423344638145 0.170441162408076 no 852 0.165285367270121 561 NA FT TRANSLATION Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A translational initiation translation initiation factor activity cytosolic ribosome|cytosolic small ribosomal subunit|ribosome YHR149C YHR149C SKG6 hom Verified 0.95210826860428 0.170521038003749 no 853 0.172187757837039 571 NA FT SITE OF POLARIZED GROWTH Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p biological_process molecular_function cell cortex|incipient cellular bud site|integral to membrane|cellular bud neck|cellular bud tip YLR133W YLR133W CKI1 hom Verified 0.951953638156602 0.170560247464377 no 854 0.216590010952759 717 NA FT LIPID METABOLISM Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolamine kinase activity|choline kinase activity cytoplasm YKR075C_p YKR075C YKR075C hom Uncharacterized 0.950761798351223 0.170862654573874 no 855 0.177406003735454 623 NA FT NUCLEUS Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YGL156W YGL156W AMS1 hom Verified 0.949681908046176 0.171136952660514 no 856 0.16851154580677 566 NA FT CARBOHYDRATE METABOLISM Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway oligosaccharide catabolic process|cellular carbohydrate metabolic process alpha-mannosidase activity fungal-type vacuole membrane YIL071C YIL071C PCI8 hom Verified 0.947822638447854 0.171609876809899 no 857 0.184032106480675 636 NA FT NUCLEUS Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain cullin deneddylation molecular_function signalosome YFL042C_p YFL042C YFL042C hom Uncharacterized 0.947597405675586 0.171667223706826 no 858 0.177859689764121 543 NA Putative protein of unknown function; YFL042C is not an essential gene biological_process molecular_function cellular_component YKR017C YKR017C HEL1 hom Verified 0.947379682072663 0.171722670316953 no 859 0.176467827106161 617 NA RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) biological_process molecular_function cellular_component YPR124W YPR124W CTR1 hom Verified 0.947007268700731 0.171817537532093 no 860 0.283175016649657 977 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress copper ion import copper uptake transmembrane transporter activity integral to membrane|plasma membrane YDR383C YDR383C NKP1 hom Verified 0.946624109698009 0.17191517697552 no 861 0.181976300540617 636 NA FT CELL CYCLE|CELL DIVISION NUCLEUS|CHROMOSOME KINETOCHORE Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 biological_process molecular_function kinetochore YLR015W YLR015W BRE2 hom Verified 0.946101050836345 0.172048523928807 no 862 0.207395932942403 720 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L histone H3-K4 methylation|telomere maintenance|histone H3-K4 trimethylation|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YOR304C-A_p YOR304C-A YOR304C-A hom Uncharacterized 0.945515931677852 0.172197770552537 no 863 0.187186634091014 633 NA FT SITE OF POLARIZED GROWTH Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck biological_process molecular_function cellular bud|mating projection tip|cellular bud neck|cytoplasm YLR241W_p YLR241W YLR241W hom Uncharacterized 0.942005334829941 0.173094954300551 no 864 0.152715040552792 499 NA Putative protein of unknown function, may be involved in detoxification biological_process molecular_function integral to membrane YDL025C YDL025C RTK1 hom Verified 0.941918856556866 0.173117092583631 no 865 0.2172704328126 713 NA FT PROTEIN PHOSPHORYLATION Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress biological_process protein serine/threonine kinase activity|protein kinase activity cellular_component YOL103W YOL103W ITR2 hom Verified 0.940313848728016 0.173528299022712 no 866 0.176058413795268 599 NA FT PLASMA MEMBRANE Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively myo-inositol transport myo-inositol transmembrane transporter activity integral to membrane|plasma membrane|membrane YIR025W YIR025W MND2 hom Verified 0.939904372063718 0.173633307261538 no 867 0.186735024718997 644 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS|CHROMOSOME SEGREGATION NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|reciprocal meiotic recombination|regulation of mitotic metaphase/anaphase transition|protein ubiquitination|mitotic sister chromatid segregation molecular_function nucleus|anaphase-promoting complex YOR223W YOR223W YOR223W hom Verified 0.938973187771514 0.173872255286724 no 868 0.174008661447366 597 NA FT ENDOPLASMIC RETICULUM Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 biological_process molecular_function fungal-type vacuole lumen|endoplasmic reticulum YIL164C YIL164C NIT1 hom Verified 0.937934118634222 0.174139134017362 no 869 0.152923535864748 537 NA Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene biological_process nitrilase activity cellular_component YLR113W YLR113W HOG1 hom Verified 0.937582404788266 0.174229528577926 no 870 0.204042391490543 647 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING|PROTEIN PHOSPHORYLATION NUCLEUS Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter by pheromones|protein phosphorylation|hyperosmotic response|response to arsenic-containing substance|osmosensory signaling pathway|positive regulation of transcription from RNA polymerase II promoter|cellular response to heat MAP kinase activity nucleus|cytoplasm YKL139W YKL139W CTK1 hom Verified 0.937515269029413 0.174246786639584 no 871 0.201375240755177 674 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION NUCLEUS Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 mRNA 3'-end processing|phosphorylation of RNA polymerase II C-terminal domain|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|peptidyl-serine phosphorylation|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation protein kinase activity|cyclin-dependent protein kinase activity nucleoplasm|nucleolus|nucleus|polysome|carboxy-terminal domain protein kinase complex YOR270C YOR270C VPH1 hom Verified 0.937291326310506 0.174304361688499 no 872 0.177819134265207 596 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress polyphosphate metabolic process|proton transport|protein complex assembly|vacuolar acidification proton-transporting ATPase activity, rotational mechanism vacuolar proton-transporting V-type ATPase, V0 domain|fungal-type vacuole membrane|integral to membrane|fungal-type vacuole YPL156C YPL156C PRM4 hom Verified 0.93591005786054 0.174659749120258 no 873 0.146828127021056 562 NA Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift biological_process molecular_function integral to membrane YML117W YML117W NAB6 hom Verified 0.935733871939064 0.174705113168443 no 874 0.171151275178971 565 NA Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP biological_process molecular_function cytoplasm YCR020C YCR020C PET18 hom Verified 0.935201784945322 0.174842159414913 no 875 0.172244388687351 593 NA Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin thiamine metabolic process molecular_function cytosol YBR045C YBR045C GIP1 hom Verified 0.935181382797731 0.17484741562361 no 876 0.189584611663362 688 NA FT CELL WALL ORG/BIOGENESIS Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p ascospore wall assembly|regulation of phosphoprotein phosphatase activity protein phosphatase type 1 regulator activity|protein phosphatase 1 binding protein phosphatase type 1 complex|prospore membrane YDR233C YDR233C RTN1 hom Verified 0.93429813231841 0.175075063739279 no 877 0.153931373842459 556 NA FT PROTEIN COMPLEX ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum organization|endoplasmic reticulum tubular network maintenance|nuclear pore complex assembly molecular_function integral to endoplasmic reticulum membrane|mitochondrion|Golgi apparatus|endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YAL047C YAL047C SPC72 hom Verified 0.934188761992762 0.17510326581731 no 878 0.181691420896932 598 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CYTOSKELETON Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization microtubule nucleation|nuclear migration along microtubule|mitotic cell cycle spindle orientation checkpoint|mitotic spindle elongation|mitotic sister chromatid segregation structural constituent of cytoskeleton outer plaque of spindle pole body YMR164C YMR164C MSS11 hom Verified 0.934178341741882 0.175105952918869 no 879 0.179775660270627 571 NA FT TRANSCRIPTION FROM RNA POL II|CARBOHYDRATE METABOLISM NUCLEUS Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals biofilm formation|Unknown|positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YLR279W_d YLR279W YLR279W hom Dubious 0.932164573189962 0.175625740436187 no 880 0.195544249752982 715 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER161C YER161C SPT2 hom Verified 0.931377957162788 0.175829044529165 no 881 0.200514843169665 692 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION NUCLEUS Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins negative regulation of transcription from RNA polymerase II promoter|chromatin organization|RNA polyadenylation|transcription elongation from RNA polymerase II promoter DNA binding|DNA secondary structure binding nucleus YLR328W YLR328W NMA1 hom Verified 0.930609074576085 0.176027909344703 no 882 0.209143494334875 625 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication NAD biosynthetic process nicotinamide-nucleotide adenylyltransferase activity nucleus|cytoplasm YOR031W YOR031W CRS5 hom Verified 0.930142347146925 0.176148693777267 no 883 0.164694854777203 553 NA Copper-binding metallothionein, required for wild-type copper resistance response to metal ion copper ion binding cytoplasm YHR108W YHR108W GGA2 hom Verified 0.929118545235467 0.176413827262936 no 884 0.169451251560946 626 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT GOLGI APPARATUS Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|Golgi to vacuole transport|Golgi to endosome transport ubiquitin binding|phosphatidylinositol-4-phosphate binding trans-Golgi network YGR291C_d YGR291C YGR291C hom Dubious 0.928559463303484 0.176558718960261 no 885 0.188799211198032 627 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL121C YGL121C GPG1 hom Verified 0.927926936111152 0.17672273544789 no 886 0.149136052115252 537 NA FT SIGNALING Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing signal transduction|invasive growth in response to glucose limitation signal transducer activity cellular_component YGR204W YGR204W ADE3 hom Verified 0.92703933919091 0.17695305476888 no 887 0.173783110202654 602 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine purine nucleobase biosynthetic process|folic acid-containing compound metabolic process methylenetetrahydrofolate dehydrogenase (NADP+) activity|formate-tetrahydrofolate ligase activity|methenyltetrahydrofolate cyclohydrolase activity nucleus|cytoplasm YPL176C YPL176C TRE1 hom Verified 0.926461967361246 0.177102976678492 no 888 0.200164038434389 706 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT PLASMA MEMBRANE Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function fungal-type vacuole YDL089W YDL089W NUR1 hom Verified 0.92612287132298 0.177191064651557 no 889 0.189124228939712 674 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate maintenance of rDNA molecular_function nuclear periphery YLR172C YLR172C DPH5 hom Verified 0.925610369884087 0.177324251162564 no 890 0.189197997926713 649 NA Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm peptidyl-diphthamide biosynthetic process from peptidyl-histidine diphthine synthase activity cytoplasm YDR244W YDR244W PEX5 hom Verified 0.925582688782293 0.177331446598547 no 891 0.200488346837836 634 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|PEROXISOME ORGANIZATION Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions protein import into peroxisome matrix, docking peroxisome matrix targeting signal-1 binding|protein binding, bridging cytosol|peroxisomal membrane|peroxisome YGL026C YGL026C TRP5 hom Verified 0.924431998679126 0.17763072052529 no 892 0.261612884469407 928 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis tryptophan biosynthetic process tryptophan synthase activity nucleus|cytoplasm YMR180C YMR180C CTL1 hom Verified 0.923383738764285 0.17790363144203 no 893 0.161641145428538 539 NA FT RNA PROCESSING NUCLEUS RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm biological_process polynucleotide 5'-phosphatase activity nucleus|cytoplasm YDR215C_d YDR215C YDR215C hom Dubious 0.923026848676504 0.177996606876785 no 894 0.177920770117372 569 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Unknown Unknown Unknown YJL073W YJL073W JEM1 hom Verified 0.92071821940302 0.178598780725122 no 895 0.185863598936034 668 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 karyogamy involved in conjugation with cellular fusion|ER-associated protein catabolic process|protein folding in endoplasmic reticulum unfolded protein binding|chaperone binding endoplasmic reticulum|nuclear outer membrane-endoplasmic reticulum membrane network YDR379W YDR379W RGA2 hom Verified 0.918211917572108 0.179253965301383 no 896 0.158888745316212 517 NA FT SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p budding cell isotropic bud growth|pheromone-dependent signal transduction involved in conjugation with cellular fusion|actin filament organization|pseudohyphal growth|establishment of cell polarity|small GTPase mediated signal transduction|invasive growth in response to glucose limitation|budding cell apical bud growth Rho GTPase activator activity cellular bud neck split septin rings YNL098C YNL098C RAS2 hom Verified 0.918200836687322 0.179256865361665 no 897 0.231617027985685 791 NA FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM|RESPONSE TO NUTRIENT LEVELS PLASMA MEMBRANE GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes pseudohyphal growth|ascospore formation|activation of adenylate cyclase activity|regulation of protein localization|replicative cell aging|positive regulation of transcription by galactose|Ras protein signal transduction GTP binding|GTPase activity plasma membrane YGR283C YGR283C YGR283C hom Verified 0.918034351874094 0.179300440885011 no 898 0.174410105368972 626 NA FT NUCLEUS Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus biological_process S-adenosylmethionine-dependent methyltransferase activity ribosome|nucleolus YDR163W YDR163W CWC15 hom Verified 0.9176773069418 0.179393915830602 no 899 0.173689352276351 604 NA FT RNA PROCESSING NUCLEUS Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YMR002W YMR002W MIC17 hom Verified 0.917576771654933 0.179420241659749 no 900 0.188025804358277 655 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS|MITOCHONDRION Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress aerobic respiration molecular_function nucleus|cytoplasm|mitochondrial intermembrane space YDR514C YDR514C YDR514C hom Verified 0.91705343053264 0.179557321216098 no 901 0.171453133338298 606 NA FT NUCLEUS|MITOCHONDRION Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication biological_process molecular_function mitochondrion|nucleus YHL045W_d YHL045W YHL045W hom Dubious 0.91660702535842 0.179674300824686 no 902 0.172234460757218 581 NA Putative protein of unknown function; not an essential gene Unknown Unknown Unknown YOR175C YOR175C ALE1 hom Verified 0.915739336161027 0.179901814055973 no 903 0.184488363753605 632 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids glycerophospholipid biosynthetic process 1-acylglycerophosphocholine O-acyltransferase activity|O-acyltransferase activity|1-acylglycerol-3-phosphate O-acyltransferase activity integral to membrane|ribosome|endoplasmic reticulum YNL071W YNL071W LAT1 hom Verified 0.915551864302549 0.179950994043343 no 904 0.19983290679899 670 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA acetyl-CoA biosynthetic process from pyruvate dihydrolipoyllysine-residue acetyltransferase activity mitochondrion|mitochondrial pyruvate dehydrogenase complex YGL152C_d YGL152C YGL152C hom Dubious 0.915492488265208 0.17996657207621 no 905 0.194372095443733 618 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Unknown Unknown Unknown YKR105C_p YKR105C VBA5 hom Uncharacterized 0.914923015097282 0.18011602336569 no 906 0.176122897984331 619 NA Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p biological_process molecular_function integral to membrane YAR028W_p YAR028W YAR028W hom Uncharacterized 0.914842186495037 0.180137242159114 no 907 0.196530334481084 677 NA Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YLL049W YLL049W LDB18 hom Verified 0.914248769345798 0.18029307164281 no 908 0.160200890042363 564 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION CYTOSKELETON Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 establishment of mitotic spindle orientation molecular_function dynactin complex|cell cortex|spindle pole body|astral microtubule YNL319W_d YNL319W YNL319W hom Dubious 0.912925282960064 0.180640919628845 no 909 0.195277037664926 678 NA Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Unknown Unknown Unknown YAL005C YAL005C SSA1 hom Verified 0.912371548197567 0.180786580876383 no 910 0.145436934005804 511 NA FT PROTEIN LOCALIZATION|TRANSLATION|MITOCHONDRION ORGANIZATION NUCLEUS ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils cytoplasmic translation|protein folding|protein refolding|protein targeting to mitochondrion|SRP-dependent cotranslational protein targeting to membrane, translocation|protein import into nucleus, translocation|response to stress unfolded protein binding|ATPase activity fungal-type vacuole membrane|fungal-type cell wall|plasma membrane|nucleus|polysome|chaperonin-containing T-complex|cytoplasm YNL318C YNL318C HXT14 hom Verified 0.911153266834993 0.181107311803338 no 911 0.15957938305464 541 NA FT MITOCHONDRION|PLASMA MEMBRANE Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies hexose transport galactose transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YGL105W YGL105W ARC1 hom Verified 0.91113861894421 0.181111170248727 no 912 0.195170795649206 672 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress positive regulation of ligase activity|tRNA aminoacylation for protein translation enzyme activator activity|tRNA binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding methionyl glutamyl tRNA synthetase complex|cytoplasm YOR090C YOR090C PTC5 hom Verified 0.911067121486922 0.181130004350172 no 913 0.186062108431366 580 NA FT MITOCHONDRION Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p positive regulation of catalytic activity|regulation of catalytic activity|protein dephosphorylation [pyruvate dehydrogenase (lipoamide)] phosphatase activity|protein serine/threonine phosphatase activity mitochondrion YJR003C_p YJR003C YJR003C hom Uncharacterized 0.910616976806593 0.181248611158302 no 914 0.183042451744978 622 NA FT MITOCHONDRION Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis biological_process molecular_function mitochondrion YDL175C YDL175C AIR2 hom Verified 0.910017059615873 0.181406756510006 no 915 0.190951069553604 647 NA FT RNA PROCESSING NUCLEUS RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication nuclear polyadenylation-dependent CUT catabolic process|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent snoRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear polyadenylation-dependent snRNA catabolic process|ncRNA polyadenylation|tRNA modification polynucleotide adenylyltransferase activity|RNA binding|protein binding, bridging nucleolus|TRAMP complex YDR191W YDR191W HST4 hom Verified 0.909945779573787 0.181425552522545 no 916 0.181189876787875 621 NA FT KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism short-chain fatty acid metabolic process|chromatin silencing at telomere|histone deacetylation histone deacetylase activity|DNA binding nucleus|cytoplasm YIL136W YIL136W OM45 hom Verified 0.909786347188766 0.181467598059911 no 917 0.185114967775262 603 NA FT MITOCHONDRION Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YOR233W YOR233W KIN4 hom Verified 0.909572704132346 0.181523949607757 no 918 0.159671095602504 593 NA FT CELL CYCLE|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication mitotic cell cycle spindle orientation checkpoint|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity cell cortex|spindle pole body|cellular bud neck YOL058W YOL058W ARG1 hom Verified 0.909230130223851 0.18161433145323 no 919 0.203461131272981 683 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate argininosuccinate metabolic process|arginine biosynthetic process|citrulline metabolic process argininosuccinate synthase activity cytosol YDR261C YDR261C EXG2 hom Verified 0.908727498612941 0.18174699255116 no 920 0.166418806076957 598 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM PLASMA MEMBRANE Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor fungal-type cell wall organization glucan exo-1,3-beta-glucosidase activity fungal-type cell wall YFR013W YFR013W IOC3 hom Verified 0.908184800323647 0.18189029661677 no 921 0.222677545626102 776 NA FT CHROMATIN ORGANIZATION NUCLEUS Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YNL202W YNL202W SPS19 hom Verified 0.906911806404046 0.182226718613822 no 922 0.203692034551874 717 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate fatty acid catabolic process|ascospore formation 2,4-dienoyl-CoA reductase (NADPH) activity peroxisomal matrix YIL037C YIL037C PRM2 hom Verified 0.906688232741356 0.182285843934788 no 923 0.188087407960441 672 NA Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion karyogamy involved in conjugation with cellular fusion molecular_function integral to membrane YML060W YML060W OGG1 hom Verified 0.906065427405568 0.182450611534129 no 924 0.178999128807754 628 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance DNA repair|base-excision repair, AP site formation oxidized purine nucleobase lesion DNA N-glycosylase activity mitochondrion YOR345C_d YOR345C YOR345C hom Dubious 0.905874550122303 0.182501128103146 no 925 0.177371410337606 600 NA Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Unknown Unknown Unknown YGR039W_d YGR039W YGR039W hom Dubious 0.90538047342887 0.182631928389063 no 926 0.183983847494714 658 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Unknown Unknown Unknown YHR082C YHR082C KSP1 hom Verified 0.905030824562589 0.182724528676349 no 927 0.18728439316287 647 NA FT PROTEIN LOCALIZATION|SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS NUCLEUS Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress TOR signaling cascade|protein phosphorylation|Unknown|cellular protein localization|regulation of filamentous growth|negative regulation of macroautophagy protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm YNL175C YNL175C NOP13 hom Verified 0.904891010522734 0.182761564942114 no 928 0.199700271238929 693 NA FT NUCLEUS Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress biological_process RNA binding nucleolus|preribosome YOR363C YOR363C PIP2 hom Verified 0.904590954761987 0.182841064516644 no 929 0.178720388638415 614 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS NUCLEUS Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication cellular response to oleic acid|positive regulation of fatty acid beta-oxidation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription integral to membrane|nucleus YEL064C YEL064C AVT2 hom Verified 0.904136055054484 0.182961631052136 no 930 0.153423010608897 553 NA FT ENDOPLASMIC RETICULUM Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters transport transporter activity integral to membrane|endoplasmic reticulum YML017W YML017W PSP2 hom Verified 0.902149988988896 0.183488598557554 no 931 0.207077320207409 742 NA Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing biological_process molecular_function cytoplasm YOR298W YOR298W MUM3 hom Verified 0.900489398090677 0.183929932413082 no 932 0.187730522893974 602 NA FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases ascospore wall assembly|phospholipid biosynthetic process transferase activity, transferring acyl groups cellular_component YIL156W YIL156W UBP7 hom Verified 0.899394167703671 0.184221372330751 no 933 0.188563603939267 621 NA FT PROTEOLYSIS Ubiquitin-specific protease that cleaves ubiquitin-protein fusions protein deubiquitination ubiquitin-specific protease activity cytoplasm YEL065W YEL065W SIT1 hom Verified 0.898798813925451 0.184379915993187 no 934 0.156835201110526 553 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p cellular iron ion homeostasis|siderophore transport|transmembrane transport siderophore uptake transmembrane transporter activity endosome|integral to membrane|cytoplasmic membrane-bounded vesicle YCR022C_d YCR022C YCR022C hom Dubious 0.898133405798806 0.184557215665458 no 935 0.165763940795892 583 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Unknown Unknown Unknown YER071C_p YER071C TDA2 hom Uncharacterized 0.897913395913672 0.184615861170391 no 936 0.171164642995619 513 NA FT SITE OF POLARIZED GROWTH Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele biological_process molecular_function mating projection tip|cytoplasm YDL091C YDL091C UBX3 hom Verified 0.897547080918629 0.184713531251577 no 937 0.162020724113242 514 NA Subunit of the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; ortholog of fission yeast Ucp10 biological_process molecular_function cytoplasm YPL066W YPL066W RGL1 hom Verified 0.897475291457356 0.184732676144432 no 938 0.223216929555663 812 NA FF|FT SITE OF POLARIZED GROWTH Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source protein localization to bud neck molecular_function cellular bud neck|cytoplasm YGR096W YGR096W TPC1 hom Verified 0.896802438475204 0.184912173279271 no 939 0.21125326227771 663 NA FT MITOCHONDRION Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family thiamine pyrophosphate transport thiamine transmembrane transporter activity integral to membrane|integral to mitochondrial inner membrane YGR126W_p YGR126W YGR126W hom Uncharacterized 0.895820747614019 0.185174253508801 no 940 0.170718870121204 611 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS biological_process molecular_function nucleus|cytoplasm YPR130C_d YPR130C YPR130C hom Dubious 0.89559762714078 0.185233851737662 no 941 0.189886188870785 663 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YFL001W YFL001W DEG1 hom Verified 0.894941259806137 0.185409244597038 no 942 0.215685241971497 693 NA FT RNA PROCESSING NUCLEUS tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability tRNA pseudouridine synthesis pseudouridine synthase activity nucleus|cytoplasm YGL009C YGL009C LEU1 hom Verified 0.894328598614799 0.185573051401644 no 943 0.183605612951114 628 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway leucine biosynthetic process 3-isopropylmalate dehydratase activity cytosol YMR241W YMR241W YHM2 hom Verified 0.893407112677868 0.185819597528686 no 944 0.191494079501544 656 NA FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome tricarboxylic acid transport|mitochondrial citrate transport|alpha-ketoglutarate transport|mitochondrial genome maintenance tricarboxylate secondary active transmembrane transporter activity|DNA binding mitochondrial nucleoid|mitochondrion|integral to membrane|mitochondrial inner membrane YCR023C YCR023C YCR023C hom Verified 0.893357583270394 0.185832855010264 no 945 0.177587946911793 621 NA Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene biological_process molecular_function fungal-type vacuole membrane|integral to membrane YBR299W YBR299W MAL32 hom Verified 0.892477816545722 0.186068438939549 no 946 0.190688109010077 671 NA FT CARBOHYDRATE METABOLISM Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose sucrose catabolic process|maltose catabolic process sucrose alpha-glucosidase activity|alpha-glucosidase activity|maltose alpha-glucosidase activity plasma membrane YGR196C YGR196C FYV8 hom Verified 0.892175743877909 0.186149370635171 no 947 0.186305239564832 650 NA Protein of unknown function, required for survival upon exposure to K1 killer toxin biological_process molecular_function cytoplasm YGR124W YGR124W ASN2 hom Verified 0.892109612917931 0.186167091437203 no 948 0.180566545569049 618 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication asparagine biosynthetic process asparagine synthase (glutamine-hydrolyzing) activity cytoplasm YDL204W YDL204W RTN2 hom Verified 0.891984980757771 0.186200491372309 no 949 0.208869015892495 720 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication endoplasmic reticulum tubular network organization|endoplasmic reticulum tubular network maintenance molecular_function endoplasmic reticulum|cortical endoplasmic reticulum|endoplasmic reticulum tubular network YML053C_p YML053C YML053C hom Uncharacterized 0.891348372898638 0.186371152621408 no 950 0.18319866152215 571 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene biological_process molecular_function nucleus|cytoplasm YBR008C YBR008C FLR1 hom Verified 0.890536802231122 0.186588858197097 no 951 0.168270990274454 604 NA FT PLASMA MEMBRANE Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress drug transmembrane transport|transmembrane transport fluconazole transporter activity|drug transmembrane transporter activity integral to membrane|plasma membrane YAL067W-A_p YAL067W-A YAL067W-A hom Uncharacterized 0.889928216727537 0.186752215839275 no 952 0.171784167694204 594 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPL097W YPL097W MSY1 hom Verified 0.887649241944089 0.187364728543062 no 953 0.199289341662405 683 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial tyrosyl-tRNA synthetase mitochondrial tyrosyl-tRNA aminoacylation tyrosine-tRNA ligase activity mitochondrion YMR074C YMR074C YMR074C hom Verified 0.886644512999734 0.18763516028977 no 954 0.164177408343561 553 NA FT NUCLEUS Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress apoptotic process molecular_function nucleus|cytoplasm YMR155W_p YMR155W YMR155W hom Uncharacterized 0.885691179423545 0.187891981356171 no 955 0.182103639711913 618 NA Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen biological_process molecular_function integral to membrane YCR006C_d YCR006C YCR006C hom Dubious 0.885484478698578 0.187947693633359 no 956 0.194945792096949 687 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL046W-A_p YIL046W-A YIL046W-A hom Uncharacterized 0.884714199605044 0.188155397625139 no 957 0.179911198930831 609 NA Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YLR209C YLR209C PNP1 hom Verified 0.884455940924592 0.188225068182773 no 958 0.199216290902661 657 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway guanosine catabolic process|inosine catabolic process|NAD biosynthesis via nicotinamide riboside salvage pathway|nicotinate nucleotide salvage nicotinamide riboside hydrolase activity|inosine nucleosidase activity|purine-nucleoside phosphorylase activity intracellular YJR151W-A_p YJR151W-A YJR151W-A hom Uncharacterized 0.883682466277623 0.188433823988544 no 959 0.181180657414457 591 NA Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis biological_process molecular_function cellular_component YIL123W YIL123W SIM1 hom Verified 0.882873159940041 0.188652403341635 no 960 0.18071394004353 625 NA FT CELL WALL ORG/BIOGENESIS Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated fungal-type cell wall organization molecular_function fungal-type cell wall YNL090W YNL090W RHO2 hom Verified 0.882477500644917 0.188759320794811 no 961 0.193691347057213 663 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly microtubule-based process|fungal-type cell wall organization|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction GTPase activity membrane YKL026C YKL026C GPX1 hom Verified 0.880913208921374 0.189182398779276 no 962 0.194110116918787 648 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress cellular response to oxidative stress|peroxisome organization phospholipid-hydroperoxide glutathione peroxidase activity|glutathione peroxidase activity extrinsic to mitochondrial outer membrane|peroxisomal matrix YKL061W_p YKL061W BLI1 hom Uncharacterized 0.879867460869912 0.189465556945475 no 963 0.176238322010955 590 NA Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome biological_process molecular_function endosome YIL166C_p YIL166C YIL166C hom Uncharacterized 0.879748155205388 0.189497878016991 no 964 0.190674760198513 627 NA Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene transport transporter activity integral to membrane|membrane YHR041C YHR041C SRB2 hom Verified 0.878044842285034 0.18995969206744 no 965 0.23917717084755 817 NA FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus|RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly|core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor recruiting transcription factor activity|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|protein domain specific binding core mediator complex YOL052C YOL052C SPE2 hom Verified 0.877913319542698 0.189995380178706 no 966 0.204999530237998 652 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM NUCLEUS S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically spermidine biosynthetic process|spermine biosynthetic process|pantothenate biosynthetic process adenosylmethionine decarboxylase activity nucleus|cytoplasm YOL079W_d YOL079W YOL079W hom Dubious 0.877691603082948 0.190055551283062 no 967 0.210716125577447 680 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR082C YLR082C SRL2 hom Verified 0.877607461035124 0.190078389459305 no 968 0.188513435540445 593 NA FT NUCLEUS Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation nucleobase-containing compound metabolic process molecular_function nucleus|cytoplasm YMR182W-A_p YMR182W-A YMR182W-A hom Uncharacterized 0.877568025546864 0.190089093780087 no 969 0.154623298529201 505 NA Putative protein of unknown function biological_process molecular_function cellular_component YNL122C_p YNL122C YNL122C hom Uncharacterized 0.877398436117286 0.190135131149817 no 970 0.183987616610107 655 NA FT TRANSLATION MITOCHONDRION Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene biological_process molecular_function mitochondrion YLL054C_p YLL054C YLL054C hom Uncharacterized 0.876277539630447 0.190439586092585 no 971 0.196496696425991 629 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene biological_process sequence-specific DNA binding|sequence-specific DNA binding RNA polymerase II transcription factor activity cellular_component YJL197W YJL197W UBP12 hom Verified 0.87585009358599 0.190555766663692 no 972 0.181319376595866 649 NA FT PROTEOLYSIS NUCLEUS Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm biological_process ubiquitin-specific protease activity nucleus|cytoplasm YMR273C YMR273C ZDS1 hom Verified 0.875501504458616 0.190650545994526 no 973 0.210578669926502 749 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL WALL ORG/BIOGENESIS|RNA LOCALIZATION|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH NUCLEAR PORE Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication mRNA export from nucleus|positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|chromatin silencing|establishment of cell polarity|regulation of protein localization|cell aging protein phosphatase type 2A regulator activity incipient cellular bud site|nuclear pore|cellular bud neck|cellular bud tip|cytoplasm YER173W YER173W RAD24 hom Verified 0.87425496947406 0.190989708193379 no 974 0.199896114666749 635 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein reciprocal meiotic recombination|DNA damage checkpoint|nucleotide-excision repair DNA clamp loader activity Rad17 RFC-like complex|nucleus YDL156W YDL156W CMR1 hom Verified 0.87393591994075 0.191076575905415 no 975 0.204585397489086 679 NA FT NUCLEUS|CHROMOSOME DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS biological_process DNA binding nuclear chromatin|nucleus|cytoplasm YNL270C YNL270C ALP1 hom Verified 0.873744110800752 0.191128811497085 no 976 0.179326957602062 629 NA FT PLASMA MEMBRANE Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication basic amino acid transport basic amino acid transmembrane transporter activity integral to membrane|plasma membrane YPL181W YPL181W CTI6 hom Verified 0.873371200268774 0.191230391684145 no 977 0.204857408963608 706 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain negative regulation of chromatin silencing at silent mating-type cassette|positive regulation of transcription, DNA-dependent|regulation of DNA-dependent DNA replication initiation|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA methylated histone residue binding|transcription factor binding Rpd3L-Expanded complex|nucleus|Rpd3L complex YIL041W YIL041W GVP36 hom Verified 0.873311627361063 0.19124662230698 no 978 0.210184854936265 686 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis vacuole organization|endocytosis|establishment or maintenance of actin cytoskeleton polarity molecular_function integral to Golgi membrane|cytoplasm YER093C-A YER093C-A AIM11 hom Verified 0.873307668814285 0.191247700841916 no 979 0.183537891695977 637 NA Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL119C YPL119C DBP1 hom Verified 0.872932647348233 0.191349895083061 no 980 0.172685370483702 591 NA FT TRANSLATION Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress translational initiation RNA helicase activity cytoplasm YIL154C YIL154C IMP2' hom Verified 0.871278535752962 0.191801043649712 no 981 0.251408325165677 894 NA FF|FT RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat DNA repair|cellular carbohydrate metabolic process transcription coactivator activity cytoplasm YNL084C YNL084C END3 hom Verified 0.871010365119192 0.191874246811246 no 982 0.229475932112495 807 NA FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p bipolar cellular bud site selection|ascospore wall assembly|endocytosis|actin filament organization|cytokinesis|actin cortical patch assembly protein binding, bridging mating projection tip|actin cortical patch YIR032C YIR032C DAL3 hom Verified 0.870750802185129 0.191945116595198 no 983 0.197071577917848 659 NA FT PLASMA MEMBRANE Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression allantoin catabolic process ureidoglycolate hydrolase activity membrane YMR310C_p YMR310C YMR310C hom Uncharacterized 0.869877731302989 0.192183613121138 no 984 0.197351084248955 658 NA FT NUCLEUS Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity nucleus YBR293W YBR293W VBA2 hom Verified 0.869029127982428 0.192415599503229 no 985 0.191117817334538 684 NA Permease of basic amino acids in the vacuolar membrane basic amino acid transport|transmembrane transport basic amino acid transmembrane transporter activity|drug transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YOR225W_d YOR225W YOR225W hom Dubious 0.868996310603879 0.192424574370294 no 986 0.1961924707539 681 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHR112C YHR112C YHR112C hom Verified 0.868783944293643 0.19248265830425 no 987 0.182691110122244 632 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway sulfur compound metabolic process cystathionine beta-lyase activity nucleus|cytoplasm YDL069C YDL069C CBS1 hom Verified 0.868597400693845 0.192533688356298 no 988 0.200770304394834 691 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial respiratory chain complex III biogenesis|positive regulation of mitochondrial translation translation regulator activity extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial ribosome YGR204C-A_p YGR204C-A YGR204C-A hom Uncharacterized 0.868056599353711 0.192681674341855 no 989 0.177130889588064 622 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YHL047C YHL047C ARN2 hom Verified 0.867971249932897 0.192705035880986 no 990 0.18628729591538 635 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM PLASMA MEMBRANE Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C cellular iron ion homeostasis|siderophore transport|siderophore metabolic process siderophore transmembrane transporter activity endosome|integral to membrane|cytoplasmic membrane-bounded vesicle|plasma membrane YPL039W_p YPL039W YPL039W hom Uncharacterized 0.867233320580955 0.192907091437015 no 991 0.208570679942151 715 NA Putative protein of unknown function; YPL039W is not an essential gene biological_process molecular_function cellular_component YIL084C YIL084C SDS3 hom Verified 0.866413204713717 0.193131802588724 no 992 0.206285727144201 742 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of chromatin silencing at telomere|chromatin silencing|negative regulation of chromatin silencing at rDNA|positive regulation of transcription from RNA polymerase II promoter|histone deacetylation histone deacetylase activity Rpd3L-Expanded complex|Rpd3L complex YEL009C YEL009C GCN4 hom Verified 0.864319114739996 0.193706306257676 no 993 0.305121856873724 1086 NA FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|AMINO ACID METABOLISM|RIBOSOME BIOGENESIS|RESPONSE TO NUTRIENT LEVELS NUCLEUS Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels nitrogen catabolite activation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription initiation from RNA polymerase II promoter|negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels chromatin binding|TFIID-class binding transcription factor activity|sequence-specific DNA binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding transcription factor activity nucleus YJR054W YJR054W KCH1 hom Verified 0.862499563749356 0.194206336524046 no 994 0.17631076822844 570 NA Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate biological_process molecular_function integral to membrane|fungal-type vacuole YCR076C YCR076C FUB1 hom Verified 0.861571414281184 0.194461703504613 no 995 0.236815202020605 764 NA Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene biological_process proteasome binding cellular_component YER073W YER073W ALD5 hom Verified 0.861518362609648 0.194476306081944 no 996 0.201516163955235 694 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed acetate biosynthetic process aldehyde dehydrogenase [NAD(P)+] activity mitochondrion YIR035C_p YIR035C YIR035C hom Uncharacterized 0.861257078745579 0.194548234719432 no 997 0.168857299648269 599 NA FT OXIDATION-REDUCTION PROCESS Putative cytoplasmic short-chain dehydrogenase/reductase biological_process molecular_function integral to membrane|cytoplasm YDR003W-A_p YDR003W-A YDR003W-A hom Uncharacterized 0.861102172394203 0.194590886415592 no 998 0.213676811950166 700 NA Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YLR083C YLR083C EMP70 hom Verified 0.860575419589846 0.194735964356864 no 999 0.226420719513376 730 NA FT VESICLE-MEDIATED TRANSPORT|ION HOMEOSTASIS|ION HOMEOSTASIS|VACUOLAR TRANSPORT Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments cellular copper ion homeostasis|vacuolar transport|pseudohyphal growth|endosomal transport|invasive growth in response to glucose limitation molecular_function endosome|fungal-type vacuole membrane|integral to membrane YGL028C YGL028C SCW11 hom Verified 0.85954084481914 0.195021097782693 no 1000 0.206668259283737 704 NA FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p cytokinesis, completion of separation glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall YDR178W YDR178W SDH4 hom Verified 0.85860278130892 0.195279851554634 no 1001 0.224600763288655 740 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain mitochondrial electron transport, succinate to ubiquinone|cellular respiration|tricarboxylic acid cycle succinate dehydrogenase (ubiquinone) activity mitochondrial respiratory chain complex II|mitochondrion YIL088C YIL088C AVT7 hom Verified 0.856523703921242 0.195854083598523 no 1002 0.170935800884403 582 NA FT PLASMA MEMBRANE Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters amino acid transport amino acid transmembrane transporter activity integral to membrane|plasma membrane|fungal-type vacuole YBR228W YBR228W SLX1 hom Verified 0.855110028016907 0.196245119277267 no 1003 0.203844642299689 721 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p DNA-dependent DNA replication 5'-flap endonuclease activity Slx1-Slx4 complex|nucleus YHR138C YHR138C YHR138C hom Verified 0.854523876327458 0.196407392920159 no 1004 0.195265575714558 633 NA FT MEMBRANE ORGANIZATION Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress vacuole fusion, non-autophagic endopeptidase inhibitor activity cellular_component YHL034C YHL034C SBP1 hom Verified 0.853109164960032 0.196799384722583 no 1005 0.17918647653192 664 NA FT TRANSLATION NUCLEUS Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 negative regulation of translation|negative regulation of translation in response to stress RNA binding|eukaryotic initiation factor 4G binding nucleolus|cytoplasmic mRNA processing body|cytoplasm YPR020W YPR020W ATP20 hom Verified 0.851571192812497 0.19722606697212 no 1006 0.193716758579273 679 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex protein oligomerization|cristae formation|protein complex assembly|ATP synthesis coupled proton transport structural molecule activity|proton-transporting ATPase activity, rotational mechanism mitochondrion|mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) YDR448W YDR448W ADA2 hom Verified 0.850682665150457 0.197472827591315 no 1007 0.261623445061453 914 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS HISTONE ACETYLTRANSFERASE Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes chromatin silencing at rDNA|chromatin modification|regulation of transcription from RNA polymerase II promoter|positive regulation of histone acetylation|chromatin silencing at telomere chromatin binding|transcription coactivator activity|histone acetyltransferase activity|phosphatidylserine binding SLIK (SAGA-like) complex|Ada2/Gcn5/Ada3 transcription activator complex|SAGA complex YPR077C_d YPR077C YPR077C hom Dubious 0.849656396990694 0.197758073513975 no 1008 0.200309032706045 679 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Unknown Unknown Unknown YDL096C_d YDL096C OPI6 hom Dubious 0.848739541801911 0.19801311912674 no 1009 0.182554118185656 614 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Unknown Unknown Unknown YKL150W YKL150W MCR1 hom Verified 0.847883796664207 0.198251344628064 no 1010 0.231776030151141 765 NA FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis ergosterol biosynthetic process|cellular response to oxidative stress cytochrome-b5 reductase activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane|mitochondrial intermembrane space YHR121W YHR121W LSM12 hom Verified 0.847554496646261 0.198343062461261 no 1011 0.193640753281799 661 NA FT NUCLEUS Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress RNA metabolic process RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YLL014W YLL014W EMC6 hom Verified 0.845069861884417 0.199035917080146 no 1012 0.232241671444936 820 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane|endoplasmic reticulum YBR059C YBR059C AKL1 hom Verified 0.843577404085487 0.199452797663937 no 1013 0.203674246550801 701 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization regulation of endocytosis|protein phosphorylation|actin cytoskeleton organization protein kinase activity cellular bud neck|cytoplasm YDL010W YDL010W GRX6 hom Verified 0.843053502795917 0.199599260874485 no 1014 0.195162597711827 617 NA FT RESPONSE TO OXIDATIVE STRESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication cellular response to oxidative stress glutathione-disulfide reductase activity|iron ion binding|protein homodimerization activity|2 iron, 2 sulfur cluster binding integral to membrane|Golgi apparatus|Golgi lumen|endoplasmic reticulum membrane|fungal-type vacuole|cis-Golgi network YGR134W YGR134W CAF130 hom Verified 0.841776165984683 0.199956627652459 no 1015 0.181605137868908 621 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex YBR227C YBR227C MCX1 hom Verified 0.841515612008144 0.200029571330382 no 1016 0.208936559186044 721 NA FT MITOCHONDRION Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins protein refolding molecular_function mitochondrion|mitochondrial matrix YPR156C YPR156C TPO3 hom Verified 0.840742942639507 0.200245978885932 no 1017 0.181303789407021 646 NA FT PLASMA MEMBRANE Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication spermine transport spermine transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|plasma membrane YGR285C YGR285C ZUO1 hom Verified 0.839631818999648 0.200557426485587 no 1018 0.199925902671039 693 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS|MITOCHONDRION RIBOSOME Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p protein folding|ribosomal subunit export from nucleus|rRNA processing|regulation of translational fidelity unfolded protein binding mitochondrion|ribosome|nucleolus|polysome|cytoplasm YJR082C YJR082C EAF6 hom Verified 0.839395913046597 0.20062358826342 no 1019 0.179630798681807 617 NA FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 DNA repair|histone acetylation histone acetyltransferase activity nucleus|NuA4 histone acetyltransferase complex|NuA3 histone acetyltransferase complex YNR074C YNR074C AIF1 hom Verified 0.839118268098829 0.200701472872839 no 1020 0.203803462684583 692 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION|PLASMA MEMBRANE Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase response to singlet oxygen|positive regulation of apoptotic process oxidoreductase activity, acting on a sulfur group of donors, NAD or NADP as acceptor mitochondrion|nucleus|plasma membrane YAL026C YAL026C DRS2 hom Verified 0.838954234363561 0.200747495936765 no 1021 0.218572295884164 746 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS GOLGI APPARATUS Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease intracellular protein transport|post-Golgi vesicle-mediated transport|phospholipid translocation|endocytosis|ribosomal small subunit assembly phospholipid-translocating ATPase activity trans-Golgi network|integral to membrane YGL162W YGL162W SUT1 hom Verified 0.838941223234453 0.20075114674982 no 1022 0.193035155090536 629 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding nucleus YKR052C YKR052C MRS4 hom Verified 0.838665613422315 0.200828489900229 no 1023 0.177243880919334 598 NA FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication mitochondrial iron ion transport|RNA splicing iron ion transmembrane transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YER037W YER037W PHM8 hom Verified 0.838504443044145 0.20087372670845 no 1024 0.200015458472159 705 NA FT RESPONSE TO NUTRIENT LEVELS NUCLEUS Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p cellular response to phosphate starvation lysophosphatidic acid phosphatase activity nucleus|cytoplasm YNR013C YNR013C PHO91 hom Verified 0.838410562185479 0.200900079718246 no 1025 0.215800984772094 722 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth phosphate ion transport|polyphosphate metabolic process phosphate ion transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|membrane YBR170C YBR170C NPL4 hom Verified 0.836954934407109 0.201308949938752 no 1026 0.203823704483309 673 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM UBIQUITIN LIGASE COMPLEX Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) retrograde protein transport, ER to cytosol|proteasomal ubiquitin-dependent protein catabolic process|mitochondria-associated protein catabolic process|mitotic spindle disassembly|ER-associated protein catabolic process|ER-associated misfolded protein catabolic process|Unknown|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process molecular_function Doa10p ubiquitin ligase complex|integral to membrane|Cdc48p-Npl4p-Ufd1p AAA ATPase complex|Cdc48p-Npl4p-Vms1p AAA ATPase complex|nuclear outer membrane-endoplasmic reticulum membrane network YKR097W YKR097W PCK1 hom Verified 0.836622966076212 0.201402266086596 no 1027 0.200311519732794 716 NA FT CARBOHYDRATE METABOLISM Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol gluconeogenesis phosphoenolpyruvate carboxykinase (ATP) activity cytosol YML082W_p YML082W YML082W hom Uncharacterized 0.836207322487739 0.201519139855299 no 1028 0.232085345616969 799 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene sulfur compound metabolic process carbon-sulfur lyase activity nucleus|cytoplasm YFL063W_d YFL063W YFL063W hom Dubious 0.83557503154754 0.20169701005489 no 1029 0.157677493924688 527 NA Dubious open reading frame, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR249W YGR249W MGA1 hom Verified 0.834741322266552 0.201931685065973 no 1030 0.205910902006953 710 NA FT NUCLEUS Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants filamentous growth sequence-specific DNA binding|DNA binding|sequence-specific DNA binding transcription factor activity nucleus YIR037W YIR037W HYR1 hom Verified 0.834124067920401 0.202105536945571 no 1031 0.209719194586646 735 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor cellular response to oxidative stress phospholipid-hydroperoxide glutathione peroxidase activity|glutathione peroxidase activity intracellular YGR234W YGR234W YHB1 hom Verified 0.832793770038914 0.202480524458533 no 1032 0.176840767189575 620 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress response to stress nitric oxide reductase activity cytosol|mitochondrion|mitochondrial matrix|cytoplasm YBR210W YBR210W ERV15 hom Verified 0.83247473682122 0.202570516100012 no 1033 0.182013790699405 589 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle YDR097C YDR097C MSH6 hom Verified 0.831647760351389 0.202803897666728 no 1034 0.173723508500762 592 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p mismatch repair|meiotic mismatch repair guanine/thymine mispair binding|four-way junction DNA binding|ATPase activity|single base insertion or deletion binding|ATP binding MutSalpha complex|nucleus YOR316C YOR316C COT1 hom Verified 0.831221439830723 0.20292427258325 no 1035 0.218554384502418 731 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication cellular zinc ion homeostasis|cobalt ion transport|zinc ion transport cobalt ion transmembrane transporter activity|zinc ion transmembrane transporter activity|inorganic cation transmembrane transporter activity mitochondrion|integral to membrane|fungal-type vacuole YDR284C YDR284C DPP1 hom Verified 0.831165587023309 0.202940046219106 no 1036 0.198522656347576 687 NA FT LIPID METABOLISM|SIGNALING Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism phospholipid metabolic process|signal transduction diacylglycerol diphosphate phosphatase activity|phosphatidate phosphatase activity fungal-type vacuole membrane|integral to membrane YER096W YER096W SHC1 hom Verified 0.83114442852666 0.20294602187402 no 1037 0.211138790020978 705 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication ascospore formation|chitin biosynthetic process enzyme activator activity prospore membrane YHL026C_p YHL026C YHL026C hom Uncharacterized 0.826359668367839 0.204300047484855 no 1038 0.213835675016563 727 NA Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) biological_process molecular_function cellular_component YMR010W_p YMR010W YMR010W hom Uncharacterized 0.82610342904941 0.204372711335828 no 1039 0.200875402981904 674 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 biological_process molecular_function integral to membrane|cytoplasm YGR197C YGR197C SNG1 hom Verified 0.823960994150476 0.204980860747759 no 1040 0.188430956790517 643 NA FT PLASMA MEMBRANE Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance nucleobase-containing compound transport molecular_function integral to membrane|plasma membrane YPL191C_p YPL191C YPL191C hom Uncharacterized 0.822192530727631 0.205483664566771 no 1041 0.198706911703268 683 NA Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YFL003C YFL003C MSH4 hom Verified 0.822013726905371 0.20553454222685 no 1042 0.165793172215426 528 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Protein involved in meiotic recombination, required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein reciprocal meiotic recombination DNA binding nuclear chromosome YJL198W YJL198W PHO90 hom Verified 0.821356683510048 0.205721564597669 no 1043 0.209934964788718 691 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication phosphate ion transport phosphate ion transmembrane transporter activity integral to membrane|membrane YDL117W YDL117W CYK3 hom Verified 0.820054139195654 0.206092622289172 no 1044 0.203126950010389 676 NA FT CELL CYCLE|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants barrier septum assembly involved in cell cycle cytokinesis|cytokinesis molecular_function cellular bud neck contractile ring|cellular bud neck|cytoplasm YLR187W YLR187W SKG3 hom Verified 0.819985222661787 0.206112265690005 no 1045 0.205045378782907 644 NA FT SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication biological_process molecular_function cellular bud|cellular bud neck|cytoplasm YGL175C YGL175C SAE2 hom Verified 0.818867477793703 0.206431013460455 no 1046 0.189297708558005 660 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents DNA double-strand break processing|DNA catabolic process, endonucleolytic|meiotic DNA double-strand break formation|meiotic DNA double-strand break processing|gene conversion at mating-type locus, DNA double-strand break processing double-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleus|cytoplasm YJL088W YJL088W ARG3 hom Verified 0.818565316051344 0.206517231173212 no 1047 0.220320042126118 779 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine arginine biosynthetic process|ornithine metabolic process ornithine carbamoyltransferase activity cytosol YJL118W YJL118W YJL118W hom Verified 0.815881922303795 0.20728383622392 no 1048 0.186791009287294 627 NA Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein biological_process molecular_function ribosome YIL023C YIL023C YKE4 hom Verified 0.815254030308504 0.207463458120847 no 1049 0.179768389482746 604 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family zinc ion transport zinc ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YML014W YML014W TRM9 hom Verified 0.814790278147315 0.207596183368185 no 1050 0.182560891787879 626 NA FT RNA PROCESSING NUCLEUS tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses chronological cell aging|tRNA methylation|tRNA wobble uridine modification|response to stress tRNA (uracil) methyltransferase activity nucleus|cytoplasm YCR083W YCR083W TRX3 hom Verified 0.812748168156533 0.208181229145121 no 1051 0.193589216717041 663 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p cellular response to oxidative stress disulfide oxidoreductase activity mitochondrion YBL021C YBL021C HAP3 hom Verified 0.811798376927451 0.208453666778874 no 1052 0.217107798730566 681 NA FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding carbon catabolite activation of transcription from RNA polymerase II promoter|regulation of cellular respiration RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription CCAAT-binding factor complex YKR064W YKR064W OAF3 hom Verified 0.809194037767819 0.209201771941062 no 1053 0.223362407144042 745 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS|MITOCHONDRION Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress negative regulation of transcription from RNA polymerase II promoter|cellular response to oleic acid RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity mitochondrion|nucleus|cytoplasm YKR044W YKR044W UIP5 hom Verified 0.80594226993663 0.210138068466964 no 1054 0.199764416138574 672 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates biological_process molecular_function nuclear envelope YML066C YML066C SMA2 hom Verified 0.805730838906193 0.210199031888538 no 1055 0.181628441523412 602 NA FT MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS ENDOPLASMIC RETICULUM Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation ascospore wall assembly|spore membrane bending pathway molecular_function integral to membrane|prospore membrane|cytoplasm YNL289W YNL289W PCL1 hom Verified 0.802630731562556 0.211094100140466 no 1056 0.214576561502884 730 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS NUCLEUS Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth regulation of establishment or maintenance of cell polarity|G1 phase of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|septin ring organization|positive regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|incipient cellular bud site|nucleus YOL065C YOL065C INP54 hom Verified 0.802393227170844 0.211162764813222 no 1057 0.180533344799626 604 NA FT PROTEIN LOCALIZATION|LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins phosphatidylinositol dephosphorylation phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity endoplasmic reticulum YHR095W_d YHR095W YHR095W hom Dubious 0.802169907076169 0.211227340614925 no 1058 0.210698952352015 719 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR368W YLR368W MDM30 hom Verified 0.802092622544638 0.211249691099672 no 1059 0.202450282764509 697 NA FT MEMBRANE ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION MITOCHONDRION UBIQUITIN LIGASE COMPLEX F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|chronological cell aging|proteolysis involved in cellular protein catabolic process|mitochondrion organization|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|regulation of mRNA export from nucleus|mitochondrial fusion ubiquitin-protein ligase activity mitochondrion|SCF ubiquitin ligase complex YPR194C YPR194C OPT2 hom Verified 0.801469038652593 0.211430080620447 no 1060 0.208598465781334 736 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION PLASMA MEMBRANE Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles oligopeptide transport|vacuole fusion, non-autophagic oligopeptide transporter activity integral to membrane|integral to plasma membrane YGL084C YGL084C GUP1 hom Verified 0.800553037982956 0.211695223595298 no 1061 0.326899099129987 1112 NA FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport GPI anchor biosynthetic process|glycerol transport|glycerol catabolic process O-acyltransferase activity integral to membrane|endoplasmic reticulum|plasma membrane|membrane YPL105C YPL105C SYH1 hom Verified 0.799594076459761 0.211973010296353 no 1062 0.207140214530814 728 NA FT PROTEIN LOCALIZATION MITOCHONDRION Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication nuclear pore distribution molecular_function mitochondrion|ribosome|cytoplasmic mRNA processing body|extrinsic to membrane|cytoplasm YNL255C YNL255C GIS2 hom Verified 0.798734322485042 0.212222240260045 no 1063 0.211268317163588 702 NA FT TRANSLATION RIBOSOME Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 positive regulation of translation mRNA binding|translation regulator activity|single-stranded DNA binding polysomal ribosome|cytoplasm YDR162C YDR162C NBP2 hom Verified 0.798436096986859 0.212308731419208 no 1064 0.301405204422537 1036 NA FF|FT PROTEIN PHOSPHORYLATION NUCLEUS Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) negative regulation of protein kinase activity|response to heat|hyperosmotic response molecular_function nucleus|cytoplasm YAL053W YAL053W FLC2 hom Verified 0.798148861279319 0.212392054799349 no 1065 0.23420708301915 813 NA FT CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication protein folding|fungal-type cell wall biogenesis|FAD transport|transmembrane transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|cytoplasm YAR020C YAR020C PAU7 hom Verified 0.797394892635997 0.212610862278901 no 1066 0.213593373483638 703 NA Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme biological_process molecular_function cellular_component YOL017W YOL017W ESC8 hom Verified 0.797072625810137 0.212704426727244 no 1067 0.218036025769605 752 NA FT GENE SILENCING NUCLEUS Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication chromatin silencing molecular_function nucleus YOR376W_d YOR376W YOR376W hom Dubious 0.794837442182672 0.213354033910043 no 1068 0.194113827787181 619 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Unknown Unknown Unknown YKL002W YKL002W DID4 hom Verified 0.792826627570861 0.213939420199486 no 1069 0.224576827019187 787 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis protein retention in Golgi apparatus|intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport molecular_function ESCRT III complex|cytoplasm YDR436W YDR436W PPZ2 hom Verified 0.792044517946511 0.214167359485513 no 1070 0.207427453352561 691 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance cellular sodium ion homeostasis protein serine/threonine phosphatase activity cellular_component YPR003C_p YPR003C YPR003C hom Uncharacterized 0.791603465261684 0.214295962876761 no 1071 0.218953486541422 723 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene biological_process molecular_function integral to membrane|endoplasmic reticulum YGR284C YGR284C ERV29 hom Verified 0.791573277967413 0.214304766615677 no 1072 0.220685937689488 751 NA FT VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress ER to Golgi vesicle-mediated transport molecular_function integral to membrane|ER to Golgi transport vesicle YPL240C YPL240C HSP82 hom Verified 0.791329859638242 0.214375764150486 no 1073 0.230739153786484 806 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication proteasome assembly|positive regulation of telomere maintenance via telomerase|box C/D snoRNP assembly|response to stress|protein refolding|protein targeting to mitochondrion|response to osmotic stress|'de novo' protein folding unfolded protein binding|ATPase activity, coupled cytoplasm YML003W_p YML003W YML003W hom Uncharacterized 0.790690541039086 0.214562298576559 no 1074 0.194360135353195 631 NA Putative protein of unknown function biological_process molecular_function cellular_component YHR039C YHR039C MSC7 hom Verified 0.790500439772953 0.214617782746919 no 1075 0.236065997671812 838 NA FT CELL CYCLE|OXIDATION-REDUCTION PROCESS ENDOPLASMIC RETICULUM Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids reciprocal meiotic recombination molecular_function integral to membrane|endoplasmic reticulum YGR157W YGR157W CHO2 hom Verified 0.790282513082568 0.214681398481998 no 1076 0.222017400050605 721 NA FT LIPID METABOLISM Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis phosphatidylcholine biosynthetic process phosphatidylethanolamine N-methyltransferase activity integral to membrane|endoplasmic reticulum YJR010W YJR010W MET3 hom Verified 0.790164859397975 0.214715747731242 no 1077 0.216503673747612 727 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)|sulfur amino acid metabolic process sulfate adenylyltransferase (ATP) activity mitochondrion|cytoplasm YKL161C YKL161C KDX1 hom Verified 0.789545386229141 0.214896656939285 no 1078 0.238177118988506 805 NA FT PROTEIN PHOSPHORYLATION Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p biological_process protein kinase activity cellular_component YLR269C_d YLR269C YLR269C hom Dubious 0.787754059282961 0.215420288945066 no 1079 0.204352185186202 669 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR002W YPR002W PDH1 hom Verified 0.784693373741636 0.216316684384721 no 1080 0.223925924655323 787 NA FT KETONE METABOLISM MITOCHONDRION Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate propionate metabolic process|propionate catabolic process, 2-methylcitrate cycle 2-methylcitrate dehydratase activity mitochondrial outer membrane|mitochondrion|cytoplasm YOR093C_p YOR093C YOR093C hom Uncharacterized 0.78321250222608 0.216751167195239 no 1081 0.171385124520911 558 NA FT NUCLEUS Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents biological_process molecular_function integral to membrane YDR149C_d YDR149C YDR149C hom Dubious 0.782201261587138 0.217048151600597 no 1082 0.214759781960342 755 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Unknown Unknown Unknown YDR265W YDR265W PEX10 hom Verified 0.781220349580818 0.217336453535781 no 1083 0.215277618080082 737 NA FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders protein import into peroxisome matrix|protein ubiquitination ubiquitin-protein ligase activity|protein binding peroxisomal membrane YDR423C YDR423C CAD1 hom Verified 0.780580307003759 0.217524688971986 no 1084 0.225780994350381 752 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication cellular response to cadmium ion|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YJL142C_d YJL142C IRC9 hom Dubious 0.780148092810691 0.217651855644006 no 1085 0.191882253677938 662 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YJL141C; null mutant displays increased levels of spontaneous Rad52p foci Unknown Unknown Unknown YLR188W YLR188W MDL1 hom Verified 0.779057451542037 0.217972936290982 no 1086 0.227059179578392 766 NA FT NUCLEOTIDE METABOLISM MITOCHONDRION Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress oligopeptide transport|oligopeptide export from mitochondrion ATPase activity|oligopeptide-transporting ATPase activity mitochondrion|integral to membrane|mitochondrial inner membrane YDR022C YDR022C ATG31 hom Verified 0.779048074719112 0.217975697975597 no 1087 0.207102542300954 712 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|CHROMOSOME SEGREGATION CYTOSKELETON Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion autophagy|mitochondrion degradation|piecemeal microautophagy of nucleus molecular_function pre-autophagosomal structure|cytoplasm YGL127C YGL127C SOH1 hom Verified 0.777580351968114 0.218408223894936 no 1088 0.233974369231466 798 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits DNA repair|meiotic gene conversion|meiotic sister chromatid segregation|transcription from RNA polymerase II promoter molecular_function core mediator complex|nucleus YHR171W YHR171W ATG7 hom Verified 0.777076668173956 0.218556769250304 no 1089 0.268130880345426 915 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS MITOCHONDRION Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation C-terminal protein lipidation|protein modification by small protein conjugation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway APG8 activating enzyme activity|APG12 activating enzyme activity cytosol|mitochondrion|membrane|pre-autophagosomal structure YGR286C YGR286C BIO2 hom Verified 0.775653645215911 0.218976758341049 no 1090 0.206574364187086 722 NA FT KETONE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant biotin biosynthetic process biotin synthase activity mitochondrion YGR057C YGR057C LST7 hom Verified 0.774983009473442 0.219174849700731 no 1091 0.279172662179229 927 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface intracellular protein transport|vesicle-mediated transport|protein transport|Golgi to plasma membrane transport protein transporter activity vesicle coat YER060W YER060W FCY21 hom Verified 0.774928851378862 0.21919085133032 no 1092 0.2096714801395 705 NA Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function transmembrane transport nucleobase transmembrane transporter activity integral to membrane YLR335W YLR335W NUP2 hom Verified 0.774577972319135 0.219294538838316 no 1093 0.22768653248945 812 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CHROMOSOME NUCLEAR PORE Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization rRNA export from nucleus|snRNA export from nucleus|nuclear pore organization|tRNA export from nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|mRNA-binding (hnRNP) protein import into nucleus|ribosomal protein import into nucleus|snRNP protein import into nucleus|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette|protein targeting to membrane|poly(A)+ mRNA export from nucleus|protein export from nucleus|mRNA export from nucleus in response to heat stress|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery|NLS-bearing substrate import into nucleus|mRNA export from nucleus|nuclear-transcribed mRNA catabolic process, non-stop decay nucleocytoplasmic transporter activity nuclear chromatin|mitochondrion|integral to membrane|nuclear pore YGR281W YGR281W YOR1 hom Verified 0.773328610725574 0.219663963773252 no 1094 0.233854027732647 810 NA FT NUCLEOTIDE METABOLISM PLASMA MEMBRANE Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) transport|response to drug xenobiotic-transporting ATPase activity integral to membrane|plasma membrane YCR073C YCR073C SSK22 hom Verified 0.772962607723024 0.219772255194181 no 1095 0.20166753185784 679 NA FT SIGNALING|PROTEIN PHOSPHORYLATION MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p MAPK cascade involved in osmosensory signaling pathway|protein phosphorylation protein kinase activity|MAP kinase kinase kinase activity cellular_component YMR067C YMR067C UBX4 hom Verified 0.771716306610838 0.220141235272894 no 1096 0.248962541554008 842 NA FT PROTEOLYSIS NUCLEUS UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress ER-associated protein catabolic process|sporulation resulting in formation of a cellular spore protein complex binding nucleus|cytoplasm|extrinsic to membrane YGR194C YGR194C XKS1 hom Verified 0.770892823078278 0.220385230738613 no 1097 0.19049474795098 641 NA FT CARBOHYDRATE METABOLISM Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains xylulose catabolic process xylulokinase activity cytoplasm YER129W YER129W SAK1 hom Verified 0.769898725125848 0.220679985110305 no 1098 0.235281721616231 798 NA FT CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome protein phosphorylation|glucose metabolic process|DNA-dependent DNA replication protein serine/threonine kinase activity|protein kinase activity cytoplasm YGL147C YGL147C RPL9A hom Verified 0.769814673832784 0.220704917033591 no 1099 0.22286743829777 780 NA FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR139C_d YOR139C YOR139C hom Dubious 0.769221247078887 0.220880989615581 no 1100 0.249619323535516 856 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Unknown Unknown Unknown YOR161C YOR161C PNS1 hom Verified 0.768473597966394 0.221102935157404 no 1101 0.225373800073944 786 NA FT PLASMA MEMBRANE Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport biological_process choline transmembrane transporter activity|molecular_function integral to membrane|plasma membrane|integral to plasma membrane YPR160W YPR160W GPH1 hom Verified 0.767626493761865 0.221354558861278 no 1102 0.190403807822085 618 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway glycogen catabolic process glycogen phosphorylase activity cytoplasm YMR044W YMR044W IOC4 hom Verified 0.766742284537703 0.221617378818241 no 1103 0.242942147271124 815 NA FT CHROMATIN ORGANIZATION NUCLEUS Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing chromatin remodeling nucleosome binding|ATPase activity|DNA binding ISW1 complex YDR262W_p YDR262W YDR262W hom Uncharacterized 0.765762711262334 0.221908752638525 no 1104 0.191501322586761 658 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment biological_process molecular_function fungal-type vacuole YLR244C YLR244C MAP1 hom Verified 0.765481038438369 0.221992576627948 no 1105 0.237268932728042 772 NA FT PROTEOLYSIS RIBOSOME Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p negative regulation of gene expression|protein initiator methionine removal involved in protein maturation metalloaminopeptidase activity|mRNA binding cytosolic ribosome YCR004C YCR004C YCP4 hom Verified 0.765419596713164 0.22201086368492 no 1106 0.211877300011824 703 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process electron carrier activity mitochondrion|membrane raft|plasma membrane|cytoplasm YDL036C YDL036C PUS9 hom Verified 0.764718217831073 0.222219677803384 no 1107 0.202215222158897 642 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence tRNA pseudouridine synthesis pseudouridine synthase activity mitochondrion|nucleus|cytoplasm YOR127W YOR127W RGA1 hom Verified 0.762970838681532 0.222740393652419 no 1108 0.229332044851009 776 NA FT SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress budding cell isotropic bud growth|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|actin filament organization|establishment of cell polarity|small GTPase mediated signal transduction|invasive growth in response to glucose limitation|budding cell apical bud growth Rho GTPase activator activity cellular bud neck split septin rings YGR229C YGR229C SMI1 hom Verified 0.762813455217795 0.22278732775401 no 1109 0.261626816465412 906 NA FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS NUCLEUS|SITE OF POLARIZED GROWTH Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity regulation of mitotic cell cycle|regulation of fungal-type cell wall biogenesis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip YPL274W YPL274W SAM3 hom Verified 0.762700215241347 0.222821101093676 no 1110 0.261368078839146 852 NA FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p spermidine transport|putrescine transport|S-adenosylmethionine transport S-adenosylmethionine transmembrane transporter activity|spermidine transmembrane transporter activity|putrescine transmembrane transporter activity integral to membrane|plasma membrane YLR371W YLR371W ROM2 hom Verified 0.762525318587222 0.222873269006121 no 1111 0.324990218561332 1073 NA FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|CELL WALL ORG/BIOGENESIS|NUCLEOTIDE METABOLISM SITE OF POLARIZED GROWTH GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p regulation of fungal-type cell wall organization|positive regulation of endocytosis|establishment of cell polarity|small GTPase mediated signal transduction|negative regulation of cAMP-mediated signaling|activation of Rho GTPase activity phosphatidylinositol-4,5-bisphosphate binding|Rho guanyl-nucleotide exchange factor activity|signal transducer activity incipient cellular bud site|mating projection tip|cellular bud tip YBR288C YBR288C APM3 hom Verified 0.759673567435007 0.223724866224869 no 1112 0.246439772520978 847 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Golgi to vacuole transport|vesicle-mediated transport molecular_function AP-3 adaptor complex YDL176W YDL176W YDL176W hom Verified 0.758950532959528 0.223941074237936 no 1113 0.194185344621153 671 NA Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene biological_process molecular_function cellular_component YHR049W YHR049W FSH1 hom Verified 0.758104475715949 0.224194220260833 no 1114 0.191881721472679 670 NA FT NUCLEUS Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 biological_process hydrolase activity nucleus|cytoplasm YLR152C_p YLR152C YLR152C hom Uncharacterized 0.758000001877993 0.224225490804383 no 1115 0.21891052275249 781 NA Putative protein of unknown function; YLR152C is not an essential gene biological_process molecular_function integral to membrane YLR296W_d YLR296W YLR296W hom Dubious 0.755035634354808 0.225113800714552 no 1116 0.224573546792298 752 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPL072W YPL072W UBP16 hom Verified 0.749028481484016 0.226920019533419 no 1117 0.204271151942757 690 NA FT PROTEOLYSIS MITOCHONDRION Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria protein deubiquitination ubiquitin-specific protease activity mitochondrial outer membrane|cytoplasm YOR209C YOR209C NPT1 hom Verified 0.748478152055782 0.227085899221653 no 1118 0.292786436341478 1031 NA FF|FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|GENE SILENCING|RESPONSE TO OXIDATIVE STRESS NUCLEUS Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus chromatin silencing at rDNA|replicative cell aging|nicotinate nucleotide salvage|chromatin silencing at telomere nicotinate phosphoribosyltransferase activity nucleus YNL293W YNL293W MSB3 hom Verified 0.747452685147158 0.22739517662581 no 1119 0.22653223847183 766 NA FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress actin filament organization|exocytosis Rab GTPase activator activity incipient cellular bud site|polarisome|cellular bud tip YDR173C YDR173C ARG82 hom Verified 0.747151917891047 0.227485932012636 no 1120 0.221414953250776 750 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM NUCLEUS Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes protein stabilization|negative regulation of transcription from RNA polymerase II promoter|phosphatidylinositol phosphorylation|regulation of arginine metabolic process|inositol phosphate biosynthetic process|positive regulation of transcription from RNA polymerase II promoter inositol-1,4,5-trisphosphate 3-kinase activity|inositol-1,4,5-trisphosphate 6-kinase activity|phosphatidylinositol 3-kinase activity|protein binding, bridging|inositol tetrakisphosphate 6-kinase activity|inositol tetrakisphosphate 3-kinase activity|inositol-1,3,4,5,6-pentakisphosphate kinase activity nucleus YLR451W YLR451W LEU3 hom Verified 0.746950204343769 0.227546809742872 no 1121 0.22478784962652 745 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YGL205W YGL205W POX1 hom Verified 0.746657121922445 0.22763527920931 no 1122 0.196558287202444 669 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix fatty acid beta-oxidation using acyl-CoA oxidase|fatty acid beta-oxidation acyl-CoA oxidase activity peroxisomal matrix YJL049W_p YJL049W YJL049W hom Uncharacterized 0.745467742581984 0.227994502348006 no 1123 0.179953917669464 597 NA FT PROTEIN LOCALIZATION Putative protein of unknown function; YJL049W is a non-essential gene biological_process molecular_function cellular_component YML038C YML038C YMD8 hom Verified 0.744955610401973 0.228149277587435 no 1124 0.230325802867449 759 NA FT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Putative nucleotide sugar transporter, has similarity to Vrg4p UDP-glucose transport|nucleotide-sugar transport nucleotide-sugar transmembrane transporter activity COPI-coated vesicle|integral to membrane YOL118C_d YOL118C YOL118C hom Dubious 0.743224213904162 0.22867297292501 no 1125 0.246686015359473 811 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR108W-A_p YPR108W-A YPR108W-A hom Uncharacterized 0.742778532269829 0.228807887367156 no 1126 0.239265692488039 794 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YDR387C_p YDR387C YDR387C hom Uncharacterized 0.742503008503735 0.228891314842747 no 1127 0.201178575329172 679 NA Putative transporter, member of the sugar porter family; YDR387C is not an essential gene transport|transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YFR043C YFR043C IRC6 hom Verified 0.741381216177584 0.229231165191703 no 1128 0.231068900780785 787 NA Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function cellular_component YKR016W YKR016W FCJ1 hom Verified 0.740454741154414 0.229512056786766 no 1129 0.217203730437621 720 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane cristae formation|protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial crista|mitochondrial crista junction|integral to mitochondrial inner membrane YNL108C_p YNL108C YNL108C hom Uncharacterized 0.739762288359652 0.229722122671908 no 1130 0.250399046586455 871 NA FT NUCLEUS Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YKL156W YKL156W RPS27A hom Verified 0.738623947099516 0.230067689364431 no 1131 0.242259978029611 822 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|cytoplasm YNL316C YNL316C PHA2 hom Verified 0.738063686790687 0.230237874551143 no 1132 0.209034325089057 720 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway L-phenylalanine biosynthetic process prephenate dehydratase activity cytoplasm YMR294W YMR294W JNM1 hom Verified 0.737827759732462 0.230309561033728 no 1133 0.264538343834829 933 NA FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION CYTOSKELETON Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B nuclear migration|establishment of mitotic spindle orientation structural constituent of cytoskeleton dynactin complex|cell cortex|spindle pole body|astral microtubule YDR004W YDR004W RAD57 hom Verified 0.736579261260728 0.230689125257394 no 1134 0.245739040488573 868 NA FF|FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p DNA recombinase assembly|telomere maintenance via recombination|heteroduplex formation|meiotic DNA recombinase assembly|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|protein heterodimerization activity nucleus|Rhp55-Rhp57 complex YPL138C YPL138C SPP1 hom Verified 0.736261907057012 0.230785661869694 no 1135 0.23493051250086 802 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein histone H3-K4 methylation|regulation of transcription, DNA-dependent|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific)|chromatin binding|methylated histone residue binding Set1C/COMPASS complex YJL171C YJL171C YJL171C hom Verified 0.735954251228759 0.230879269849139 no 1136 0.196357257801864 634 NA FT MITOCHONDRION|PLASMA MEMBRANE GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress biological_process molecular_function mitochondrion|fungal-type cell wall|plasma membrane YNL304W YNL304W YPT11 hom Verified 0.734578590557534 0.231298090362296 no 1137 0.239693241339434 805 NA FT SIGNALING|MITOCHONDRION ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells mitochondrion inheritance|endoplasmic reticulum inheritance|Golgi inheritance|Golgi localization GTPase activity incipient cellular bud site|endoplasmic reticulum|cellular bud neck|cellular bud tip YPL162C_p YPL162C YPL162C hom Uncharacterized 0.734087752128597 0.231447628859676 no 1138 0.231462554872263 792 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology biological_process molecular_function fungal-type vacuole membrane|integral to membrane YER155C YER155C BEM2 hom Verified 0.733157847667578 0.231731080660696 no 1139 0.241516913822366 805 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION MITOCHONDRION|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence negative regulation of Rho protein signal transduction|fungal-type cell wall organization|establishment of cell polarity|actin cytoskeleton organization Rho GTPase activator activity cell cortex|incipient cellular bud site|mitochondrion|plasma membrane|mating projection tip|cytoplasm|cellular bud tip YOR034C YOR034C AKR2 hom Verified 0.732302896260028 0.231991855979149 no 1140 0.210082359325544 717 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION GOLGI APPARATUS Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication endocytosis|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus YOL012C YOL012C HTZ1 hom Verified 0.731277691241813 0.232304776996756 no 1141 0.255175029520174 891 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin nuclear-transcribed mRNA catabolic process, non-stop decay|regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette chromatin binding nuclear chromatin|chromatin assembly complex YPL031C YPL031C PHO85 hom Verified 0.730029411821306 0.232686103338628 no 1142 0.272982475196901 908 NA FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES A NUCLEUS Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle regulation of transcription involved in G1/S phase of mitotic cell cycle|negative regulation of calcium-mediated signaling|regulation of protein stability|regulation of protein localization|protein phosphorylation|response to DNA damage stimulus|negative regulation of phosphate metabolic process|fungal-type cell wall organization|regulation of establishment or maintenance of cell polarity|negative regulation of glycogen biosynthetic process|negative regulation of transcription from RNA polymerase II promoter|negative regulation of sequence-specific DNA binding transcription factor activity|positive regulation of macroautophagy|negative regulation of macroautophagy protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YPL110C YPL110C GDE1 hom Verified 0.729616476532531 0.232812323992109 no 1143 0.239003962412822 823 NA FT LIPID METABOLISM|SIGNALING|CARBOHYDRATE METABOLISM Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes glycerophospholipid catabolic process glycerophosphocholine phosphodiesterase activity ribosome|cytoplasm YJR104C YJR104C SOD1 hom Verified 0.727125758326394 0.23357446035009 no 1144 0.334602495298552 1161 NA FF|FT ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid cellular copper ion homeostasis|fungal-type cell wall organization|superoxide metabolic process|cellular zinc ion homeostasis|positive regulation of sequence-specific DNA binding transcription factor activity|age-dependent response to reactive oxygen species involved in chronological cell aging superoxide dismutase activity cytosol|mitochondrion|nucleus|mitochondrial intermembrane space YJL022W_d YJL022W YJL022W hom Dubious 0.726327388015557 0.233819046656154 no 1145 0.242884104624301 790 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Unknown Unknown Unknown YOR186W_p YOR186W YOR186W hom Uncharacterized 0.726049934543146 0.233904079682948 no 1146 0.221541481419964 774 NA Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication biological_process molecular_function cellular_component YBR175W YBR175W SWD3 hom Verified 0.724610721873031 0.234345439683213 no 1147 0.230202428741123 785 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 histone H3-K4 methylation|telomere maintenance|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YPR028W YPR028W YOP1 hom Verified 0.723185641334129 0.234782919576915 no 1148 0.23638944447778 824 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress vesicle-mediated transport|endoplasmic reticulum organization|endoplasmic reticulum inheritance|nuclear pore complex assembly protein binding integral to membrane|endoplasmic reticulum|endoplasmic reticulum tubular network YIL125W YIL125W KGD1 hom Verified 0.722072699567559 0.235124890944111 no 1149 0.249153283427751 834 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA tricarboxylic acid cycle|2-oxoglutarate metabolic process oxoglutarate dehydrogenase (succinyl-transferring) activity mitochondrial nucleoid|mitochondrion|mitochondrial matrix|mitochondrial oxoglutarate dehydrogenase complex YGL027C YGL027C CWH41 hom Verified 0.721916878355763 0.235172791758227 no 1150 0.257901695083722 890 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress fungal-type cell wall biogenesis|protein N-linked glycosylation mannosyl-oligosaccharide glucosidase activity endoplasmic reticulum membrane YLR233C YLR233C EST1 hom Verified 0.718778851825483 0.236138595003674 no 1151 0.189121726295079 617 NA FT NUCLEUS|CHROMOSOME TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA telomere maintenance via telomerase|telomere maintenance|G-quadruplex DNA formation single-stranded DNA binding|RNA binding|telomeric DNA binding nucleolus|nucleus|telomerase holoenzyme complex YGL045W YGL045W RIM8 hom Verified 0.718625344200001 0.236185896662097 no 1152 0.270612157309437 924 NA FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family meiosis|protein processing|invasive growth in response to glucose limitation|ubiquitin-dependent endocytosis molecular_function internal side of plasma membrane YJL007C_d YJL007C YJL007C hom Dubious 0.717869178367631 0.236418976861597 no 1153 0.232670482063599 776 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR024W_d YOR024W YOR024W hom Dubious 0.717309042555989 0.236591714459835 no 1154 0.237640460682201 786 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR264C YDR264C AKR1 hom Verified 0.716008688463073 0.236992992018171 no 1155 0.364341672979491 1228 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication protein targeting to membrane|regulation of endocytosis|regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion|protein palmitoylation palmitoyltransferase activity integral to membrane|Golgi apparatus|membrane YKR096W YKR096W ESL2 hom Verified 0.715733348671768 0.237078007361276 no 1156 0.243506876423817 822 NA FT NUCLEUS Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain biological_process molecular_function ribosome|nucleus|cytoplasm YPL170W YPL170W DAP1 hom Verified 0.715177391341022 0.237249718701991 no 1157 0.266361353581559 946 NA FT LIPID METABOLISM Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis regulation of ergosterol biosynthetic process heme binding endosome|membrane YER044C-A YER044C-A MEI4 hom Verified 0.715171005907295 0.237251691284921 no 1158 0.219672450309335 756 NA FT CELL CYCLE NUCLEUS|CHROMOSOME Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores meiotic DNA double-strand break formation molecular_function condensed nuclear chromosome YLR216C YLR216C CPR6 hom Verified 0.715167575495334 0.23725275100868 no 1159 0.20230861846639 675 NA Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress protein folding|protein refolding unfolded protein binding|peptidyl-prolyl cis-trans isomerase activity cytoplasm YNL191W YNL191W DUG3 hom Verified 0.715048068215968 0.237289670851517 no 1160 0.226266265266177 779 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process peptidase activity|omega peptidase activity|gamma-glutamyltransferase activity|carbon-nitrogen ligase activity, with glutamine as amido-N-donor cytoplasm YLR043C YLR043C TRX1 hom Verified 0.713996523760008 0.237614664507268 no 1161 0.234089229880405 821 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS ENDOMEMBRANE SYSTEM|NUCLEUS|GOLGI APPARATUS Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress vacuole inheritance|regulation of DNA replication|cell redox homeostasis|protein deglutathionylation|retrograde vesicle-mediated transport, Golgi to ER|cellular response to oxidative stress|ER to Golgi vesicle-mediated transport|vacuole fusion, non-autophagic disulfide oxidoreductase activity cytosol|fungal-type vacuole YJL078C YJL078C PRY3 hom Verified 0.713382223423098 0.237804635075231 no 1162 0.199367851723408 695 NA Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p biological_process molecular_function fungal-type cell wall YDL114W_p YDL114W YDL114W hom Uncharacterized 0.713048990457487 0.237907721226152 no 1163 0.241629330999469 814 NA FT OXIDATION-REDUCTION PROCESS Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene biological_process molecular_function integral to membrane YLR136C YLR136C TIS11 hom Verified 0.712600794580189 0.238046409968973 no 1164 0.22302583922951 766 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS NUCLEUS mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress cellular iron ion homeostasis|nuclear-transcribed mRNA catabolic process mRNA binding nucleus|cytoplasm YJL027C_p YJL027C YJL027C hom Uncharacterized 0.71236574198959 0.238119161833921 no 1165 0.223309432733627 755 NA Putative protein of unknown function biological_process molecular_function cellular_component YOR214C_p YOR214C YOR214C hom Uncharacterized 0.711658823951425 0.238338035663054 no 1166 0.233523490695538 785 NA Putative protein of unknown function; YOR214C is not an essential gene biological_process molecular_function fungal-type cell wall YBR213W YBR213W MET8 hom Verified 0.708433139480596 0.239338159441212 no 1167 0.261080491936434 887 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis siroheme biosynthetic process|sulfate assimilation precorrin-2 dehydrogenase activity|ferrochelatase activity cellular_component YOL039W YOL039W RPP2A hom Verified 0.70804780907351 0.239457784261261 no 1168 0.258680804610866 896 NA FT TRANSLATION RIBOSOME Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm translational elongation|cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJL067W_d YJL067W YJL067W hom Dubious 0.707575897319094 0.239604332501554 no 1169 0.219960227085021 766 NA Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL016W_p YJL016W YJL016W hom Uncharacterized 0.706350354294406 0.239985143168736 no 1170 0.246356065761946 824 NA Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species biological_process molecular_function cytoplasm YFR045W_p YFR045W YFR045W hom Uncharacterized 0.705217285351372 0.240337512953453 no 1171 0.215869991436985 764 NA FT MITOCHONDRION Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white transport transporter activity integral to membrane|mitochondrial inner membrane YDR323C YDR323C PEP7 hom Verified 0.704424065989886 0.240584361546944 no 1172 0.298740070033499 999 NA FF|FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Golgi to vacuole transport|vacuole inheritance|vesicle fusion|vesicle docking involved in exocytosis|Golgi to endosome transport phosphatidylinositol-3-phosphate binding external side of endosome membrane|cytoplasm YNL046W_p YNL046W YNL046W hom Uncharacterized 0.704380180694333 0.240598022609019 no 1173 0.224331561868398 747 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) biological_process molecular_function endoplasmic reticulum YER176W YER176W ECM32 hom Verified 0.704119425557991 0.240679201834028 no 1174 0.222175222680615 768 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes regulation of translational termination RNA helicase activity|DNA helicase activity polysome YOL061W YOL061W PRS5 hom Verified 0.702085865813261 0.241312808156846 no 1175 0.245916630292946 834 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress fungal-type cell wall organization|5-phosphoribose 1-diphosphate biosynthetic process ribose phosphate diphosphokinase activity cytoplasm YGL185C_p YGL185C YGL185C hom Uncharacterized 0.700272917568098 0.241878440779761 no 1176 0.280147796048724 933 NA FT OXIDATION-REDUCTION PROCESS Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm metabolic process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor cytoplasm YPR111W YPR111W DBF20 hom Verified 0.699652147301809 0.242072283819316 no 1177 0.213487884686332 735 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs cytokinesis after mitosis|protein phosphorylation|barrier septum assembly involved in cell cycle cytokinesis|regulation of mRNA catabolic process|exit from mitosis protein serine/threonine kinase activity spindle pole body|cellular bud neck|cytoplasm YLR455W YLR455W YLR455W hom Verified 0.698240400507478 0.242513432320112 no 1178 0.257404168071867 905 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YHR066W YHR066W SSF1 hom Verified 0.697691158878165 0.242685179208953 no 1179 0.224680158302088 765 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication conjugation with cellular fusion|ribosomal large subunit assembly|regulation of cell size rRNA binding nucleolus YPL073C_d YPL073C YPL073C hom Dubious 0.696240597241719 0.243139083698299 no 1180 0.225997891103989 761 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Unknown Unknown Unknown YBR197C YBR197C YBR197C hom Verified 0.694687336941469 0.243625632773467 no 1181 0.244783766338747 844 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YBR201W YBR201W DER1 hom Verified 0.692522930863776 0.244304495270099 no 1182 0.247687529103912 848 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM UBIQUITIN LIGASE COMPLEX Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p ER-associated protein catabolic process molecular_function integral to membrane|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YHR086W YHR086W NAM8 hom Verified 0.691352321341183 0.244672079485314 no 1183 0.246965024370961 791 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS|MITOCHONDRION RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function mRNA splicing, via spliceosome|positive regulation of mRNA splicing, via spliceosome|mRNA splice site selection mRNA binding|RNA binding commitment complex|U1 snRNP|U2-type prespliceosome YGR244C YGR244C LSC2 hom Verified 0.691050538621312 0.244766890827386 no 1184 0.247264181274697 853 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate tricarboxylic acid cycle|succinyl-CoA metabolic process succinate-CoA ligase (ADP-forming) activity mitochondrion YBL042C YBL042C FUI1 hom Verified 0.689274553228571 0.245325254183777 no 1185 0.281550195894117 1025 NA FF|FT PLASMA MEMBRANE High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress uridine transport|transmembrane transport uridine transmembrane transporter activity integral to membrane|plasma membrane YMR265C_p YMR265C YMR265C hom Uncharacterized 0.688818186582588 0.245468844690742 no 1186 0.241913779893307 849 NA Putative protein of unknown function biological_process molecular_function cellular_component YOL138C YOL138C RTC1 hom Verified 0.688412197317473 0.245596622480853 no 1187 0.248179201488587 849 NA Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif biological_process molecular_function ribosome|fungal-type vacuole|Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YER089C YER089C PTC2 hom Verified 0.688398692938439 0.245600873354152 no 1188 0.21062013146068 686 NA FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication endoplasmic reticulum unfolded protein response|inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of cyclin-dependent protein kinase activity|protein dephosphorylation|DNA damage checkpoint protein serine/threonine phosphatase activity nucleus|cytoplasm YDR336W_p YDR336W YDR336W hom Uncharacterized 0.687592162361589 0.245854822595073 no 1189 0.256777716108035 867 NA Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene biological_process molecular_function cellular_component YNL043C_d YNL043C YNL043C hom Dubious 0.687533735119517 0.24587322483362 no 1190 0.250414040381196 854 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Unknown Unknown Unknown YLR341W YLR341W SPO77 hom Verified 0.68517944628123 0.246615346084863 no 1191 0.251135407312646 847 NA FT CELL WALL ORG/BIOGENESIS Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function intracellular YBL006C YBL006C LDB7 hom Verified 0.685154169612056 0.24662332032603 no 1192 0.319998901729644 1102 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CELL WALL ORG/BIOGENESIS NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions ATP-dependent chromatin remodeling|cell wall mannoprotein biosynthetic process|nucleosome disassembly|transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YIR042C_p YIR042C YIR042C hom Uncharacterized 0.685084191276015 0.246645397694441 no 1193 0.233427272118261 771 NA Putative protein of unknown function; YIR042C is a non-essential gene biological_process molecular_function cellular_component YGR236C YGR236C SPG1 hom Verified 0.684908808436024 0.246700733634345 no 1194 0.244195075638191 846 NA FT MITOCHONDRION Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YFR020W_p YFR020W YFR020W hom Uncharacterized 0.684083525043445 0.246961212254134 no 1195 0.250731032964982 833 NA Protein of unknown function; mRNA identified as translated by ribosome profiling data biological_process molecular_function cellular_component YNL327W YNL327W EGT2 hom Verified 0.683114627850707 0.247267206441831 no 1196 0.251625625743385 847 NA FT CELL CYCLE|CELL DIVISION Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner cytokinesis cellulase activity fungal-type cell wall|cellular bud|cell septum YDR205W YDR205W MSC2 hom Verified 0.682589711786262 0.247433068470526 no 1197 0.252739872571998 890 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids cellular zinc ion homeostasis|zinc ion transport|transmembrane transport zinc ion transmembrane transporter activity|metal ion transmembrane transporter activity integral to membrane|endoplasmic reticulum YJR051W YJR051W OSM1 hom Verified 0.68256456696858 0.247441015176581 no 1198 0.238106348115824 798 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication cellular response to anoxia fumarate reductase (NADH) activity mitochondrion YNR027W YNR027W BUD17 hom Verified 0.68213527541774 0.247576708457176 no 1199 0.259197143417051 884 NA FT CELL CYCLE|CELL DIVISION|COFACTOR METABOLISM NUCLEUS Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) pyridoxal phosphate biosynthetic process|cellular bud site selection pyridoxal kinase activity nucleus|cytoplasm YPR170C_d YPR170C YPR170C hom Dubious 0.679555520181531 0.248392970875147 no 1200 0.306155775301632 1005 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Unknown Unknown Unknown YDR245W YDR245W MNN10 hom Verified 0.678239189072877 0.248810024287751 no 1201 0.253030346037514 880 NA FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family N-glycan processing|cell wall mannoprotein biosynthetic process|substituted mannan metabolic process|barrier septum assembly alpha-1,6-mannosyltransferase activity alpha-1,6-mannosyltransferase complex YDR080W YDR080W VPS41 hom Verified 0.677866473964958 0.248928179366058 no 1202 0.250482739228169 867 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole organization|vesicle-mediated transport|protein transport|vacuole fusion, non-autophagic|vacuolar protein processing Rab guanyl-nucleotide exchange factor activity|phosphatidylinositol binding endosome|fungal-type vacuole membrane|HOPS complex YGL083W YGL083W SCY1 hom Verified 0.67758609962635 0.24901708101041 no 1203 0.233782383914181 788 NA FT PROTEIN PHOSPHORYLATION Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase biological_process molecular_function clathrin-coated vesicle YGL163C YGL163C RAD54 hom Verified 0.676754379304881 0.249280903876669 no 1204 0.318908460502151 1063 NA FF|FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress positive regulation of endodeoxyribonuclease activity|DNA geometric change|heteroduplex formation|telomere maintenance via recombination|double-strand break repair via synthesis-dependent strand annealing|chromatin remodeling|double-strand break repair via single-strand annealing DNA-dependent ATPase activity|DNA translocase activity nucleus YKL128C YKL128C PMU1 hom Verified 0.676279367268063 0.249431645017435 no 1205 0.211911238628574 705 NA FT NUCLEUS Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant biological_process molecular_function nucleus|cytoplasm YEL025C_p YEL025C YEL025C hom Uncharacterized 0.676265408115162 0.24943607557178 no 1206 0.211033484603774 699 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YFR044C YFR044C DUG1 hom Verified 0.676255274893944 0.249439291823752 no 1207 0.254023417167188 881 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|PROTEOLYSIS MITOCHONDRION Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) glutathione catabolic process metallodipeptidase activity|omega peptidase activity mitochondrion|ribosome|cytoplasm YMR216C YMR216C SKY1 hom Verified 0.674491810395919 0.249999345317211 no 1208 0.319752083817917 1076 NA FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 cellular cation homeostasis|cellular ion homeostasis|response to drug|protein phosphorylation|positive regulation of protein import into nucleus|regulation of cell size|mRNA splice site selection protein serine/threonine kinase activity|protein kinase activity cytoplasm YLR028C YLR028C ADE16 hom Verified 0.674490759476595 0.249999679274407 no 1209 0.242627650156771 791 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine ascospore formation|'de novo' IMP biosynthetic process|purine nucleotide biosynthetic process|aerobic respiration phosphoribosylaminoimidazolecarboxamide formyltransferase activity|IMP cyclohydrolase activity cytosol YCR102C_p YCR102C YCR102C hom Uncharacterized 0.673213595766903 0.250405706452114 no 1210 0.25126031957307 894 NA FT OXIDATION-REDUCTION PROCESS Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family response to copper ion molecular_function cellular_component YLR307C-A_p YLR307C-A YLR307C-A hom Uncharacterized 0.671554690019682 0.250933615843488 no 1211 0.230459218031913 780 NA Putative protein of unknown function biological_process molecular_function cellular_component YER170W YER170W ADK2 hom Verified 0.670592666971021 0.251240027632832 no 1212 0.251817582341598 883 NA FT NUCLEOTIDE METABOLISM MITOCHONDRION Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background nucleotide metabolic process nucleoside triphosphate adenylate kinase activity|adenylate kinase activity mitochondrion|mitochondrial inner membrane YDR453C YDR453C TSA2 hom Verified 0.670395159984822 0.251302959607684 no 1213 0.23423328057439 777 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress cellular response to oxidative stress|cell redox homeostasis peroxiredoxin activity|thioredoxin peroxidase activity cytoplasm YOR179C YOR179C SYC1 hom Verified 0.670273175781363 0.251341831797284 no 1214 0.248980729538518 866 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication termination of RNA polymerase II transcription, exosome-dependent|termination of RNA polymerase II transcription, poly(A)-coupled RNA binding mRNA cleavage and polyadenylation specificity factor complex|nucleus YER046W YER046W SPO73 hom Verified 0.670194158971469 0.251367013445795 no 1215 0.239787927637757 815 NA FT CELL WALL ORG/BIOGENESIS Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis ascospore wall assembly|ascospore formation molecular_function cytosol YDR408C YDR408C ADE8 hom Verified 0.669973724346385 0.251437270195674 no 1216 0.249158084519536 845 NA FT NUCLEOTIDE METABOLISM NUCLEUS Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process|adenine biosynthetic process phosphoribosylglycinamide formyltransferase activity nucleus|cytoplasm YBR238C YBR238C YBR238C hom Verified 0.667143681954198 0.252340180197375 no 1217 0.291387349004369 998 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span aerobic respiration molecular_function mitochondrion|integral to mitochondrial membrane|cytoplasm YMR317W_p YMR317W YMR317W hom Uncharacterized 0.666486289218661 0.252550162227279 no 1218 0.244676263983864 866 NA Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene biological_process molecular_function cellular_component YDR465C YDR465C RMT2 hom Verified 0.666119771117342 0.25266727408295 no 1219 0.278584059168704 930 NA FT NUCLEUS Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress peptidyl-arginine methylation protein-arginine N5-methyltransferase activity nucleus|cytoplasm YAR003W YAR003W SWD1 hom Verified 0.66393800016115 0.253364996847236 no 1220 0.287144570407203 981 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 histone H3-K4 methylation|telomere maintenance|regulation of transcription, DNA-dependent|chromatin silencing at telomere histone methyltransferase activity (H3-K4 specific) Set1C/COMPASS complex YPL221W YPL221W FLC1 hom Verified 0.663521047249968 0.253498452110497 no 1221 0.246483737085874 843 NA FT CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance protein folding|fungal-type cell wall biogenesis|FAD transport FAD transmembrane transporter activity integral to membrane|endoplasmic reticulum|fungal-type vacuole|cellular bud neck YBL005W YBL005W PDR3 hom Verified 0.662431802040859 0.253847264020087 no 1222 0.247329534581293 860 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YNL323W YNL323W LEM3 hom Verified 0.661559248699161 0.254126865750714 no 1223 0.34252711982541 1164 NA FF|FT MEMBRANE ORGANIZATION|SIGNALING ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane phospholipid translocation|cell surface receptor signaling pathway phospholipid-translocating ATPase activity integral to membrane|endoplasmic reticulum|plasma membrane|cytoplasm YDL200C YDL200C MGT1 hom Verified 0.660995139504416 0.254307715312638 no 1224 0.273843182862358 938 NA FT RESPONSE TO DNA DAMAGE NUCLEUS DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage DNA dealkylation involved in DNA repair methylated-DNA-[protein]-cysteine S-methyltransferase activity nucleus YBL104C YBL104C SEA4 hom Verified 0.659702329470972 0.25472243575099 no 1225 0.346087524056815 1161 NA Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites biological_process molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane|cytoplasm YDR304C YDR304C CPR5 hom Verified 0.658425183292686 0.25513247883225 no 1226 0.246802352989445 877 NA FT ENDOPLASMIC RETICULUM Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER biological_process peptidyl-prolyl cis-trans isomerase activity endoplasmic reticulum|cytoplasm YDR231C YDR231C COX20 hom Verified 0.65499042006294 0.25623696101626 no 1227 0.258074577587945 877 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase mitochondrial respiratory chain complex IV assembly|protein processing|aerobic respiration unfolded protein binding mitochondrion|mitochondrial inner membrane YPL112C YPL112C PEX25 hom Verified 0.654942865111964 0.256252270258597 no 1228 0.257885915635778 917 NA FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p protein import into peroxisome matrix|peroxisome organization molecular_function peroxisomal membrane YNL063W YNL063W MTQ1 hom Verified 0.654358017072688 0.256440587860676 no 1229 0.265361718908067 928 NA FT TRANSLATION MITOCHONDRION S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene translational readthrough S-adenosylmethionine-dependent methyltransferase activity mitochondrion YMR041C YMR041C ARA2 hom Verified 0.652836240938996 0.256930928459206 no 1230 0.247458436474697 825 NA FT OXIDATION-REDUCTION PROCESS NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase dehydro-D-arabinono-1,4-lactone biosynthetic process D-arabinose 1-dehydrogenase [NAD(P)+] activity cellular_component YPL196W YPL196W OXR1 hom Verified 0.651623539799926 0.257322029136523 no 1231 0.244859892862987 879 NA FT RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes cellular response to oxidative stress molecular_function mitochondrion YBL071C_d YBL071C YBL071C hom Dubious 0.650783287534676 0.257593194998206 no 1232 0.252405475101585 841 NA Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Unknown Unknown Unknown YMR042W YMR042W ARG80 hom Verified 0.650037142288021 0.257834115018267 no 1233 0.254730022850251 848 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding nucleus YPL261C_d YPL261C YPL261C hom Dubious 0.649583376902797 0.257980686767868 no 1234 0.255911695350184 893 NA Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Unknown Unknown Unknown YOR142W YOR142W LSC1 hom Verified 0.648266932944748 0.258406158667826 no 1235 0.23833704697722 843 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated tricarboxylic acid cycle|succinyl-CoA metabolic process succinate-CoA ligase (ADP-forming) activity mitochondrial nucleoid|mitochondrion YGL217C_d YGL217C YGL217C hom Dubious 0.647829703643485 0.258547550632521 no 1236 0.238676795984733 800 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Unknown Unknown Unknown YHR104W YHR104W GRE3 hom Verified 0.646819230806669 0.258874472307024 no 1237 0.257088711852075 896 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress galactose catabolic process|cellular response to oxidative stress|arabinose catabolic process|D-xylose catabolic process|response to stress D-xylose:NADP reductase activity|mRNA binding|glucose 1-dehydrogenase (NADP+) activity|alditol:NADP+ 1-oxidoreductase activity nucleus|cytoplasm YFR015C YFR015C GSY1 hom Verified 0.646393254660179 0.259012353858835 no 1238 0.221937781071258 769 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress glycogen biosynthetic process glycogen (starch) synthase activity mitochondrion|cytoplasm YLL038C YLL038C ENT4 hom Verified 0.64613344088516 0.259096469996254 no 1239 0.206792779979948 664 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome endocytosis|actin filament organization|establishment of protein localization to plasma membrane|actin cortical patch assembly clathrin binding actin cortical patch YPR138C YPR138C MEP3 hom Verified 0.644857118697274 0.259509891341562 no 1240 0.250196219684975 854 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease nitrogen utilization|ammonium transport ammonium transmembrane transporter activity integral to membrane|plasma membrane YBR204C YBR204C LDH1 hom Verified 0.644579584630339 0.2595998341744 no 1241 0.255561154170287 847 NA FT LIPID METABOLISM Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) lipid homeostasis hydrolase activity, acting on ester bonds|serine hydrolase activity|triglyceride lipase activity lipid particle YLR431C YLR431C ATG23 hom Verified 0.643548414853509 0.25993415509288 no 1242 0.27296978321947 976 NA FT PROTEIN LOCALIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p protein localization to pre-autophagosomal structure|positive regulation of macroautophagy|CVT pathway molecular_function pre-autophagosomal structure|extrinsic to membrane YNL266W_d YNL266W YNL266W hom Dubious 0.64324797110361 0.260031605284795 no 1243 0.24228302785184 827 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Unknown Unknown Unknown YHR109W YHR109W CTM1 hom Verified 0.642548467644925 0.260258565135615 no 1244 0.235801721234655 804 NA Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth peptidyl-lysine methylation [cytochrome c]-lysine N-methyltransferase activity cytosol YBR216C YBR216C YBP1 hom Verified 0.642436275117288 0.260294976452695 no 1245 0.250648947870446 823 NA FT RESPONSE TO OXIDATIVE STRESS Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication cellular response to oxidative stress molecular_function cytoplasm YLR361C YLR361C DCR2 hom Verified 0.641595251372928 0.260568008512208 no 1246 0.23444181488047 790 NA FT CELL CYCLE|SIGNALING Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START endoplasmic reticulum unfolded protein response|traversing start control point of mitotic cell cycle|protein dephosphorylation hydrolase activity, acting on ester bonds|phosphoprotein phosphatase activity|phosphoric ester hydrolase activity cellular_component YJR061W_p YJR061W YJR061W hom Uncharacterized 0.640824494158126 0.260818358484783 no 1247 0.266328873097418 901 NA Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL257W_p YPL257W YPL257W hom Uncharacterized 0.640530269505488 0.260913958340521 no 1248 0.251614808455034 859 NA FT CELL CYCLE Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene biological_process molecular_function integral to membrane YJL083W YJL083W TAX4 hom Verified 0.640083184078229 0.261059260407851 no 1249 0.228936211308105 761 NA FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|RESPONSE TO NUTRIENT LEVELS EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication autophagy|fungal-type cell wall organization|inositol lipid-mediated signaling|CVT pathway|cellular response to starvation molecular_function pre-autophagosomal structure YDR519W YDR519W FPR2 hom Verified 0.639508992470867 0.261245932786173 no 1250 0.287045085226514 985 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress biological_process FK506 binding|peptidyl-prolyl cis-trans isomerase activity endoplasmic reticulum membrane YLR460C_p YLR460C YLR460C hom Uncharacterized 0.639445654318194 0.261266528515057 no 1251 0.241126523300353 832 NA FT OXIDATION-REDUCTION PROCESS Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine biological_process molecular_function cellular_component YBR007C YBR007C DSF2 hom Verified 0.635600823597183 0.262518316274792 no 1252 0.276276641894061 1003 NA FF Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular_component YOR239W YOR239W ABP140 hom Verified 0.635551265155683 0.262534471372092 no 1253 0.256846468244659 864 NA FT RNA PROCESSING|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift actin filament bundle assembly|tRNA methylation tRNA (cytosine-3-)-methyltransferase activity|actin filament binding|protein binding, bridging actin filament|actin filament bundle|mating projection tip|actin cortical patch YDL121C_p YDL121C YDL121C hom Uncharacterized 0.635475868999223 0.262559050042693 no 1254 0.244481229344822 813 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein biological_process molecular_function endoplasmic reticulum YNL074C YNL074C MLF3 hom Verified 0.635437970420544 0.262571405184633 no 1255 0.250120629876684 873 NA Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide response to drug molecular_function cytoplasm YER026C YER026C CHO1 hom Verified 0.634873117735631 0.262755585479039 no 1256 0.277847511594606 958 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM|MITOCHONDRION Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline phosphatidylserine biosynthetic process CDP-diacylglycerol-serine O-phosphatidyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YHR116W YHR116W COX23 hom Verified 0.633963047312623 0.263052469119446 no 1257 0.261588976209258 867 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs mitochondrial respiratory chain complex assembly molecular_function mitochondrion|cytoplasm YIL076W YIL076W SEC28 hom Verified 0.631843409544117 0.263744602439056 no 1258 0.253264805819079 880 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport|vesicle coating molecular_function endosome|COPI vesicle coat YLL010C YLL010C PSR1 hom Verified 0.630793608021782 0.264087741677695 no 1259 0.276985151820149 949 NA FT PLASMA MEMBRANE Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p response to stress phosphoprotein phosphatase activity plasma membrane YJL030W YJL030W MAD2 hom Verified 0.628334750854463 0.264892335628773 no 1260 0.249416365147302 858 NA FT CELL CYCLE|CELL DIVISION ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME NUCLEAR PORE|KINETOCHORE Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I chromosome decondensation|mitotic cell cycle spindle assembly checkpoint|mitotic cell cycle G2/M transition decatenation checkpoint molecular_function mitotic checkpoint complex|condensed nuclear chromosome kinetochore|nuclear pore YJL161W_p YJL161W FMP33 hom Uncharacterized 0.627960805717084 0.265014808034194 no 1261 0.243650091140427 824 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YDR522C YDR522C SPS2 hom Verified 0.627903261768421 0.265033657058048 no 1262 0.24366439736971 855 NA FT CELL CYCLE|CELL WALL ORG/BIOGENESIS PLASMA MEMBRANE Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component ascospore wall assembly|ascospore formation molecular_function fungal-type cell wall|plasma membrane YDR270W YDR270W CCC2 hom Verified 0.627018012181506 0.265323714113302 no 1263 0.296805519599789 1037 NA FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism cellular iron ion homeostasis|copper ion export|transmembrane transport copper ion binding|cation-transporting ATPase activity integral to membrane|trans-Golgi network transport vesicle membrane YAR047C_d YAR047C YAR047C hom Dubious 0.625411013729414 0.265850667711539 no 1264 0.2549259880536 874 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR031W YGR031W IMO32 hom Verified 0.625360928591731 0.265867099733199 no 1265 0.25742517792614 895 NA FT MITOCHONDRION Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 biological_process molecular_function mitochondrion YOL003C YOL003C PFA4 hom Verified 0.624973618293787 0.265994186566337 no 1266 0.267428653636405 913 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases protein palmitoylation palmitoyltransferase activity integral to membrane|endoplasmic reticulum YMR226C YMR226C YMR226C hom Verified 0.624646841959539 0.266101434521977 no 1267 0.259394402021171 911 NA FT OXIDATION-REDUCTION PROCESS NUCLEUS NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments metabolic process oxidoreductase activity|oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor|carbonyl reductase (NADPH) activity integral to membrane|ribosome|nucleus|cytoplasm YLR134W YLR134W PDC5 hom Verified 0.623989250199665 0.266317322414646 no 1268 0.261389135766049 884 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism L-phenylalanine catabolic process|glucose catabolic process to ethanol|pyruvate metabolic process|tryptophan catabolic process|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway pyruvate decarboxylase activity nucleus|cytoplasm YDL059C YDL059C RAD59 hom Verified 0.623795700556027 0.266380881794913 no 1269 0.283373145850097 982 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p DNA strand renaturation|telomere maintenance via recombination|double-strand break repair via break-induced replication|double-strand break repair via single-strand annealing protein binding nucleus YCR011C YCR011C ADP1 hom Verified 0.623564112289863 0.266456942687123 no 1270 0.247103397772115 878 NA FT NUCLEOTIDE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative ATP-dependent permease of the ABC transporter family of proteins transport|transmembrane transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|endoplasmic reticulum|cytoplasm YOR198C YOR198C BFR1 hom Verified 0.623313469610288 0.266539274027332 no 1271 0.301031054064322 1024 NA FT CELL CYCLE ENDOMEMBRANE SYSTEM Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity meiosis|mRNA metabolic process|regulation of mitosis RNA binding ribonucleoprotein complex|polysome|nuclear outer membrane-endoplasmic reticulum membrane network YOR040W YOR040W GLO4 hom Verified 0.622309962229934 0.266869035883902 no 1272 0.248386465346622 824 NA FT KETONE METABOLISM|CARBOHYDRATE METABOLISM MITOCHONDRION Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate cellular carbohydrate metabolic process|methylglyoxal catabolic process to D-lactate hydroxyacylglutathione hydrolase activity mitochondrion|mitochondrial matrix YOL001W YOL001W PHO80 hom Verified 0.620832308583287 0.267354981544227 no 1273 0.295715754150542 1029 NA FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|ION HOMEOSTASIS|PROTEIN PHOSPHORYLATION|ION HOMEOSTASIS|RESPONSE TO NUTRIENT LEVELS NUCLEUS Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling negative regulation of phosphate metabolic process|regulation of cyclin-dependent protein kinase activity|negative regulation of calcium-mediated signaling|negative regulation of transcription from RNA polymerase II promoter|cellular metal ion homeostasis|regulation of protein localization|negative regulation of macroautophagy cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus YLR264W YLR264W RPS28B hom Verified 0.620829456739367 0.267355919841694 no 1274 0.255704003207829 865 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YNL097C-B_p YNL097C-B YNL097C-B hom Uncharacterized 0.620031123663039 0.267618648157162 no 1275 0.243539209966005 799 NA Putative protein of unknown function biological_process molecular_function cellular_component YIL141W_d YIL141W YIL141W hom Dubious 0.618826917388666 0.268015193823972 no 1276 0.259550569871191 922 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER181C_d YER181C YER181C hom Dubious 0.618604582799991 0.268088441042822 no 1277 0.26072610064443 898 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Unknown Unknown Unknown YIL153W YIL153W RRD1 hom Verified 0.617887585011012 0.268324721647198 no 1278 0.285739165151999 987 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress DNA repair|mitotic spindle organization in nucleus|G1/S transition of mitotic cell cycle|response to osmotic stress|regulation of transcription from RNA polymerase II promoter in response to stress peptidyl-prolyl cis-trans isomerase activity|protein phosphatase type 2A regulator activity nuclear chromatin|nucleus|cytoplasm YBR180W YBR180W DTR1 hom Verified 0.617721758012141 0.268379383453684 no 1279 0.264563144651102 870 NA FT CELL WALL ORG/BIOGENESIS Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation ascospore wall assembly|amine transport|transmembrane transport amine transmembrane transporter activity integral to membrane|prospore membrane YHR076W YHR076W PTC7 hom Verified 0.616150458732522 0.268897611010621 no 1280 0.284059062466621 953 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope biological_process protein serine/threonine phosphatase activity nuclear envelope|mitochondrion YIR029W YIR029W DAL2 hom Verified 0.615658084681701 0.269060103392204 no 1281 0.25712348370618 890 NA Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process allantoicase activity cellular_component YLR338W_d YLR338W OPI9 hom Dubious 0.615585629522776 0.269084019069654 no 1282 0.317038160113909 1093 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Unknown Unknown Unknown YIL002W-A_p YIL002W-A YIL002W-A hom Uncharacterized 0.615469218977705 0.269122445579656 no 1283 0.251703067988369 874 NA Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YNL117W YNL117W MLS1 hom Verified 0.614905720309428 0.269308492447074 no 1284 0.279859718391066 934 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic glyoxylate cycle malate synthase activity peroxisomal matrix|cytoplasm YMR003W YMR003W AIM34 hom Verified 0.61443881255019 0.269462697338826 no 1285 0.267210891649501 891 NA FT MITOCHONDRION Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YNL285W_d YNL285W YNL285W hom Dubious 0.613654791751488 0.269721734171369 no 1286 0.259593005136611 896 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR193C YPR193C HPA2 hom Verified 0.613343761827285 0.269824531555842 no 1287 0.254327857807997 896 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity protein homotetramerization|histone acetylation histone acetyltransferase activity|protein homodimerization activity cytoplasm YBR230C YBR230C OM14 hom Verified 0.613309419819844 0.269835883011923 no 1288 0.282784628644783 963 NA FT MITOCHONDRION Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron biological_process molecular_function mitochondrion|integral to mitochondrial outer membrane YML078W YML078W CPR3 hom Verified 0.612237259469574 0.2701903966317 no 1289 0.279039904171607 972 NA FT MITOCHONDRION Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria protein folding|apoptotic process peptidyl-prolyl cis-trans isomerase activity mitochondrion YBR103W YBR103W SIF2 hom Verified 0.611292866442743 0.270502856368581 no 1290 0.279698265563913 978 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|SIGNALING|GENE SILENCING NUCLEUS|CHROMOSOME WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus negative regulation of meiosis|negative regulation of chromatin silencing at telomere|regulation of transcription, DNA-dependent|histone deacetylation|positive regulation of stress-activated MAPK cascade molecular_function Rpd3L-Expanded complex|nucleus|Set3 complex YNL045W YNL045W LAP2 hom Verified 0.610779464533356 0.270672795079779 no 1291 0.256224658979538 896 NA FT KETONE METABOLISM|LIPID METABOLISM|PROTEOLYSIS NUCLEUS Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus cellular lipid metabolic process|protein catabolic process aminopeptidase activity|leukotriene-A4 hydrolase activity|epoxide hydrolase activity nucleus|cytoplasm YFL025C YFL025C BST1 hom Verified 0.61016721577725 0.270875522287628 no 1292 0.346424062659423 1160 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules protein retention in ER lumen|vesicle organization|ER-associated protein catabolic process|ER to Golgi vesicle-mediated transport|GPI anchor metabolic process phosphatidylinositol deacylase activity integral to membrane|endoplasmic reticulum YLR012C_p YLR012C YLR012C hom Uncharacterized 0.609876389237069 0.270971847025807 no 1293 0.30443956014458 1058 NA Putative protein of unknown function; YLR012C is not an essential gene biological_process molecular_function cellular_component YNL127W YNL127W FAR11 hom Verified 0.609774891225851 0.271005468232356 no 1294 0.325839144372234 1121 NA FT CELL CYCLE|SIGNALING Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YIL117C YIL117C PRM5 hom Verified 0.608880739968917 0.271301745640314 no 1295 0.254581429445993 895 NA Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling biological_process molecular_function integral to membrane YJR073C YJR073C OPI3 hom Verified 0.606818755460004 0.271985599916965 no 1296 0.307495566795282 1074 NA FF|FT LIPID METABOLISM MITOCHONDRION Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis phosphatidylcholine biosynthetic process phosphatidyl-N-dimethylethanolamine N-methyltransferase activity|phosphatidyl-N-methylethanolamine N-methyltransferase activity mitochondrion|integral to membrane YMR283C YMR283C RIT1 hom Verified 0.605501130202087 0.27242303697636 no 1297 0.236795715297374 847 NA FT RNA PROCESSING 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA charged-tRNA amino acid modification transferase activity, transferring pentosyl groups cytoplasm YJL134W YJL134W LCB3 hom Verified 0.602458960217932 0.273434336686896 no 1298 0.265409779248675 893 NA FT LIPID METABOLISM|SIGNALING ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication calcium-mediated signaling|sphingolipid biosynthetic process sphingosine-1-phosphate phosphatase activity integral to membrane|endoplasmic reticulum YBR231C YBR231C SWC5 hom Verified 0.601253940849667 0.273835431063896 no 1299 0.302440490018731 1057 NA FF|FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress chromatin remodeling|histone exchange molecular_function Swr1 complex|nucleus YBL062W_d YBL062W YBL062W hom Dubious 0.601014106852115 0.273915295241759 no 1300 0.302816949332985 1040 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIR033W YIR033W MGA2 hom Verified 0.599464673790518 0.274431530256258 no 1301 0.222361195452811 736 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting positive regulation of transcription from RNA polymerase II promoter in response to hypoxia|cellular response to cold|positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|mRNA stabilization|positive regulation of transcription from RNA polymerase II promoter in response to cobalt ion|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette molecular_function integral to endoplasmic reticulum membrane|nucleus YJR149W_p YJR149W YJR149W hom Uncharacterized 0.599263887476319 0.274498462707767 no 1302 0.239692566105148 786 NA FT OXIDATION-REDUCTION PROCESS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YPR153W YPR153W YPR153W hom Verified 0.597496861352384 0.275087850939504 no 1303 0.258606925217542 867 NA Putative protein of unknown function biological_process molecular_function integral to membrane YKR074W YKR074W AIM29 hom Verified 0.597371940828423 0.275129541521895 no 1304 0.260225330140319 901 NA Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function cytoplasm YMR305C YMR305C SCW10 hom Verified 0.597116680883305 0.275214740845949 no 1305 0.277903609737959 919 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOPLASMIC RETICULUM Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p conjugation with cellular fusion glucosidase activity fungal-type cell wall|extracellular region|endoplasmic reticulum|cytoplasm YBR289W YBR289W SNF5 hom Verified 0.594823329236213 0.275980785780949 no 1306 0.302032101678385 982 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS NUCLEUS|CHROMOSOME RSC COMPLEX Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|double-strand break repair via homologous recombination|chromatin remodeling|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II activating transcription factor binding SWI/SNF complex|nucleus YCR031C YCR031C RPS14A hom Verified 0.594347751362574 0.276139773366205 no 1307 0.273791215065485 915 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS RIBOSOME Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome|mRNA binding small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YGR232W YGR232W NAS6 hom Verified 0.594195888886849 0.27619055106786 no 1308 0.280402946197995 965 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 proteolysis|proteasome regulatory particle assembly molecular_function cytosol|nucleus|proteasome regulatory particle YLR308W YLR308W CDA2 hom Verified 0.594026184643715 0.276247299877446 no 1309 0.273268675048229 947 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall ascospore wall assembly chitin deacetylase activity chitosan layer of spore wall YHR176W YHR176W FMO1 hom Verified 0.593345997272891 0.27647481078273 no 1310 0.277434857974978 954 NA FT OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins protein folding N,N-dimethylaniline monooxygenase activity endoplasmic reticulum membrane YDR445C_d YDR445C YDR445C hom Dubious 0.59305876459279 0.276570912727388 no 1311 0.299526107021371 1022 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YBL029C-A YBL029C-A YBL029C-A hom Verified 0.592123753083038 0.276883861052757 no 1312 0.282558930883065 963 NA FT PLASMA MEMBRANE Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica biological_process molecular_function plasma membrane YLR312W-A YLR312W-A MRPL15 hom Verified 0.589961099186691 0.277608364969199 no 1313 0.284616941202287 1001 NA FT RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YMR246W YMR246W FAA4 hom Verified 0.589671556371464 0.2777054340593 no 1314 0.272234217489008 942 NA FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication long-chain fatty acid transport|long-chain fatty-acyl-CoA metabolic process long-chain fatty acid-CoA ligase activity lipid particle|cytoplasm YOR011W YOR011W AUS1 hom Verified 0.588919307858157 0.277957702468722 no 1315 0.291098274383848 997 NA FT NUCLEOTIDE METABOLISM MITOCHONDRION|PLASMA MEMBRANE Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen sterol import sterol-transporting ATPase activity mitochondrion|integral to membrane|plasma membrane YOL060C YOL060C MAM3 hom Verified 0.588890634922575 0.277967320221138 no 1316 0.265249926382794 916 NA FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS Protein required for normal mitochondrial morphology, has similarity to hemolysins cellular manganese ion homeostasis|mitochondrion organization molecular_function fungal-type vacuole membrane|integral to membrane YKL208W YKL208W CBT1 hom Verified 0.586912235714167 0.278631325850465 no 1317 0.277635353008764 945 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS MITOCHONDRION Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p cytochrome complex assembly|mRNA processing|mRNA stabilization molecular_function mitochondrion YDL068W_d YDL068W YDL068W hom Dubious 0.586006934182485 0.278935427522929 no 1318 0.300141221262062 1056 NA Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YER187W_p YER187W YER187W hom Uncharacterized 0.585450003217007 0.279122587516404 no 1319 0.260042649708499 883 NA Putative protein of unknown function; induced in respiratory-deficient cells biological_process molecular_function cellular_component YDR179W-A_p YDR179W-A YDR179W-A hom Uncharacterized 0.584613303081304 0.279403880332097 no 1320 0.28267834502117 922 NA Putative protein of unknown function biological_process molecular_function cellular_component YER118C YER118C SHO1 hom Verified 0.583971056086995 0.279619892715358 no 1321 0.269773927415395 917 NA FT SIGNALING SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p signal transduction involved in filamentous growth|establishment of cell polarity|osmosensory signaling pathway|cellular response to heat|cytokinesis osmosensor activity|MAP-kinase scaffold activity integral to membrane|plasma membrane|plasma membrane part|cellular bud|cellular bud neck|mating projection tip YDR272W YDR272W GLO2 hom Verified 0.582922107663867 0.279972868663535 no 1322 0.288018915826041 986 NA FT KETONE METABOLISM|CARBOHYDRATE METABOLISM Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate cellular carbohydrate metabolic process|methylglyoxal catabolic process to D-lactate hydroxyacylglutathione hydrolase activity cytoplasm YPR004C YPR004C AIM45 hom Verified 0.582534800422842 0.280103253912232 no 1323 0.279114434905317 951 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response biological_process molecular_function mitochondrion YDL239C YDL239C ADY3 hom Verified 0.580120303503137 0.280916746385395 no 1324 0.277146082213195 915 NA FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|MITOCHONDRION ORGANIZATION NUCLEUS|CYTOSKELETON Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication mitochondrion inheritance|ascospore wall assembly|sporulation resulting in formation of a cellular spore molecular_function spindle pole body|prospore membrane YGL054C YGL054C ERV14 hom Verified 0.580046395351365 0.28094166552708 no 1325 0.395083789327844 1304 NA FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication ascospore formation|ER to Golgi vesicle-mediated transport|axial cellular bud site selection molecular_function integral to membrane|ER to Golgi transport vesicle|endoplasmic reticulum membrane YNR008W YNR008W LRO1 hom Verified 0.579839309395594 0.281011493072256 no 1326 0.285283634448617 978 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase lipid storage|triglyceride biosynthetic process phospholipid:diacylglycerol acyltransferase activity lipid particle|perinuclear endoplasmic reticulum YDR380W YDR380W ARO10 hom Verified 0.579186643075083 0.281231621208493 no 1327 0.264958731576079 897 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway L-phenylalanine catabolic process|methionine catabolic process to 3-methylthiopropanol|tryptophan catabolic process|branched-chain amino acid catabolic process to alcohol via Ehrlich pathway|aromatic amino acid family catabolic process to alcohol via Ehrlich pathway|leucine catabolic process pyruvate decarboxylase activity|carboxy-lyase activity|phenylpyruvate decarboxylase activity cytoplasm YER123W YER123W YCK3 hom Verified 0.578308288963885 0.281527999591443 no 1328 0.319251145130623 1108 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN PHOSPHORYLATION ENDOMEMBRANE SYSTEM|NUCLEUS|PLASMA MEMBRANE Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway vesicle-mediated transport|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity fungal-type vacuole membrane|plasma membrane|nucleus|fungal-type vacuole YHR022C-A_p YHR022C-A YHR022C-A hom Uncharacterized 0.578001928614587 0.281631408572993 no 1329 0.275542255986835 951 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YLR437C YLR437C DIF1 hom Verified 0.576857240791384 0.282017948900594 no 1330 0.263933473645016 922 NA FT NUCLEUS Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein biological_process molecular_function cytoplasm YEL056W YEL056W HAT2 hom Verified 0.576133229702852 0.282262566159873 no 1331 0.261225440488818 873 NA FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing histone acetylation|chromatin assembly or disassembly|chromatin silencing at telomere histone acetyltransferase activity|histone binding histone acetyltransferase complex|nucleus|cytoplasm YKL201C YKL201C MNN4 hom Verified 0.574811883924077 0.282709264321057 no 1332 0.278463991172393 950 NA FT CARBOHYDRATE METABOLISM Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication protein O-linked glycosylation|protein N-linked glycosylation|response to stress molecular_function membrane YER079W_p YER079W YER079W hom Uncharacterized 0.574788387830157 0.282717210554693 no 1333 0.272820140058023 911 NA FT NUCLEUS Putative protein of unknown function biological_process molecular_function nucleus|cytoplasm YPR140W YPR140W TAZ1 hom Verified 0.574742121863646 0.282732857732108 no 1334 0.282345773301111 941 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome cardiolipin acyl-chain remodeling|mitochondrial ATP synthesis coupled electron transport|protein complex assembly|inner mitochondrial membrane organization|cardiolipin metabolic process|phospholipid biosynthetic process 1-acylglycerophosphocholine O-acyltransferase activity mitochondrial outer membrane|mitochondrion|mitochondrial inner membrane|integral to mitochondrial outer membrane YPL262W YPL262W FUM1 hom Verified 0.574646275201483 0.282765274454115 no 1335 0.273278250514073 933 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria fumarate metabolic process|tricarboxylic acid cycle fumarate hydratase activity cytosol|mitochondrion|mitochondrial matrix YLL027W YLL027W ISA1 hom Verified 0.574210861835457 0.282912560003878 no 1336 0.302276481222954 1059 NA FT KETONE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins biotin biosynthetic process|iron-sulfur cluster assembly iron-sulfur cluster binding|iron ion binding mitochondrial matrix YLR433C YLR433C CNA1 hom Verified 0.573213433421043 0.283250094935483 no 1337 0.258240242055629 907 NA FT SIGNALING|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|ION HOMEOSTASIS Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|fungal-type cell wall organization|cellular ion homeostasis calcium-dependent protein serine/threonine phosphatase activity calcineurin complex|cytoplasm YGL002W YGL002W ERP6 hom Verified 0.571812600435868 0.283724469942354 no 1338 0.300832050843157 1018 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|integral to membrane YBR226C_d YBR226C YBR226C hom Dubious 0.57148128273745 0.283836722249473 no 1339 0.319200116487279 1050 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Unknown Unknown Unknown YDR297W YDR297W SUR2 hom Verified 0.570995241442051 0.284001434246195 no 1340 0.298169851464762 1025 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis sphingolipid metabolic process sphingosine hydroxylase activity integral to membrane|endoplasmic reticulum membrane YIL058W_d YIL058W YIL058W hom Dubious 0.570105354923841 0.284303121702965 no 1341 0.287512621401852 978 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR097C_p YOR097C YOR097C hom Uncharacterized 0.56954256322027 0.28449399720038 no 1342 0.301869983877734 1058 NA Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene biological_process molecular_function cellular_component YOR342C YOR342C YOR342C hom Verified 0.569362420868079 0.284555106910747 no 1343 0.293827361672428 1021 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication biological_process molecular_function nucleus|cytoplasm YER175W-A_p YER175W-A YER175W-A hom Uncharacterized 0.567752523580428 0.285101510705672 no 1344 0.278096717060187 973 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR075W YDR075W PPH3 hom Verified 0.56663986916226 0.285479440888284 no 1345 0.262131204650467 890 NA FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Unknown|signal transduction involved in meiotic recombination checkpoint|double-strand break repair via homologous recombination|protein dephosphorylation|positive regulation of nitrogen compound metabolic process protein serine/threonine phosphatase activity condensed nuclear chromosome|nucleus|cytoplasm YAR002C-A YAR002C-A ERP1 hom Verified 0.561654554286017 0.28717570167064 no 1346 0.252447507736118 864 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication ER to Golgi vesicle-mediated transport molecular_function mitochondrion|ER to Golgi transport vesicle YLL026W YLL026W HSP104 hom Verified 0.56083698277351 0.287454336318387 no 1347 0.290812376147258 1003 NA FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress trehalose metabolism in response to heat stress|protein folding in endoplasmic reticulum|protein unfolding|chaperone cofactor-dependent protein refolding|inheritance of oxidatively modified proteins involved in replicative cell aging|cellular heat acclimation ADP binding|unfolded protein binding|ATPase activity, coupled|chaperone binding|ATP binding TRC complex|nucleus|cytoplasm YOR301W YOR301W RAX1 hom Verified 0.560752100183995 0.287483272281201 no 1348 0.244536425837044 820 NA FT CELL CYCLE|SIGNALING|CELL DIVISION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily cellular bud site selection molecular_function integral to membrane|cellular bud neck YLL029W YLL029W FRA1 hom Verified 0.560094257062282 0.287707573723315 no 1349 0.29784706603999 1028 NA FT TRANSCRIPTION FROM RNA POL II|PROTEOLYSIS Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation negative regulation of transcription from RNA polymerase II promoter in response to iron molecular_function cytosol|cytoplasm YGR052W_p YGR052W FMP48 hom Uncharacterized 0.559957537553853 0.287754200659637 no 1350 0.32578448776075 1087 NA FT PROTEIN PHOSPHORYLATION MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation protein phosphorylation protein kinase activity mitochondrion YFR017C YFR017C IGD1 hom Verified 0.559839668433805 0.28779440170906 no 1351 0.294995402115173 1001 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication negative regulation of glycogen catabolic process enzyme inhibitor activity cytoplasm YMR177W YMR177W MMT1 hom Verified 0.559717251957426 0.287836156511621 no 1352 0.288424686070854 993 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p cellular iron ion homeostasis molecular_function mitochondrion|integral to membrane YLR017W YLR017W MEU1 hom Verified 0.559062096538989 0.288059670843833 no 1353 0.269287416770955 911 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression L-methionine salvage from methylthioadenosine|glutamate biosynthetic process S-methyl-5-thioadenosine phosphorylase activity|mRNA binding cytoplasm YBR157C YBR157C ICS2 hom Verified 0.558467465574834 0.288262607400447 no 1354 0.27865027168201 937 NA Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization biological_process molecular_function cellular_component YLR037C YLR037C PAU23 hom Verified 0.557366434057691 0.288638547015714 no 1355 0.288154071431115 958 NA FT CELL WALL ORG/BIOGENESIS Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth biological_process molecular_function fungal-type cell wall YOR271C_p YOR271C FSF1 hom Uncharacterized 0.557072740337478 0.288738865712525 no 1356 0.274244675597977 935 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis biological_process molecular_function mitochondrion|integral to membrane YER086W YER086W ILV1 hom Verified 0.557054733656236 0.288745016894979 no 1357 0.324083678457828 1093 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation threonine catabolic process|isoleucine biosynthetic process L-threonine ammonia-lyase activity mitochondrion YBR114W YBR114W RAD16 hom Verified 0.556992305117813 0.288766343308662 no 1358 0.297461790720049 1012 NA FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex nucleotide-excision repair, DNA damage recognition|protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity|damaged DNA binding|DNA-dependent ATPase activity nucleotide-excision repair factor 4 complex|Cul3-RING ubiquitin ligase complex YML052W YML052W SUR7 hom Verified 0.556637090569994 0.288887703404687 no 1359 0.261415870572481 913 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION MITOCHONDRION|PLASMA MEMBRANE Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants endocytosis|ascospore formation molecular_function cell cortex|eisosome|mitochondrion|membrane raft|integral to membrane|plasma membrane|actin cortical patch YDR281C YDR281C PHM6 hom Verified 0.556107787490138 0.289068585879665 no 1360 0.306114212195232 1066 NA Protein of unknown function, expression is regulated by phosphate levels biological_process molecular_function fungal-type vacuole YFR011C YFR011C AIM13 hom Verified 0.555791945022212 0.289176546324347 no 1361 0.273767604878735 913 NA FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial crista junction|cytoplasm|mitochondrial intermembrane space YDL057W_p YDL057W YDL057W hom Uncharacterized 0.555322742106118 0.289336963025394 no 1362 0.261359130526355 909 NA Putative protein of unknown function; YDL057W is not an essential gene biological_process molecular_function cellular_component YKR056W YKR056W TRM2 hom Verified 0.552620772792305 0.29026155734096 no 1363 0.283660528642156 985 NA FT RNA PROCESSING|RESPONSE TO DNA DAMAGE tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair double-strand break repair|DNA repair|tRNA modification tRNA (m5U54) methyltransferase activity|double-stranded DNA specific 5'-3' exodeoxyribonuclease activity|single-stranded DNA specific endodeoxyribonuclease activity cellular_component YNL173C YNL173C MDG1 hom Verified 0.550049845158669 0.291142592931799 no 1364 0.306243870585421 1046 NA FT SIGNALING PLASMA MEMBRANE Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations pheromone-dependent signal transduction involved in conjugation with cellular fusion molecular_function membrane raft|plasma membrane YPL195W YPL195W APL5 hom Verified 0.550013657627528 0.291155003006503 no 1365 0.314836718154382 1051 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function vesicle-mediated transport|Golgi to vacuole transport protein binding AP-3 adaptor complex YLR280C_d YLR280C YLR280C hom Dubious 0.549326193052045 0.291390807546447 no 1366 0.261044260794165 878 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL085C_d YOL085C YOL085C hom Dubious 0.549248321423114 0.291417523607152 no 1367 0.291720490596792 989 NA Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Unknown Unknown Unknown YIL086C_d YIL086C YIL086C hom Dubious 0.548360374591652 0.29172223960992 no 1368 0.278730478633527 947 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGR051C_d YGR051C YGR051C hom Dubious 0.548024935585665 0.291837390575255 no 1369 0.280701867716086 945 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Unknown Unknown Unknown YBL106C YBL106C SRO77 hom Verified 0.547689298955288 0.291952630570689 no 1370 0.361457361416584 1201 NA FF|FT VESICLE-MEDIATED TRANSPORT PLASMA MEMBRANE Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication exocytosis|Golgi to plasma membrane transport molecular_function plasma membrane YGL203C YGL203C KEX1 hom Verified 0.547635908893473 0.291970963867843 no 1371 0.29227535953029 999 NA FT PROTEOLYSIS GOLGI APPARATUS Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins protein processing serine-type carboxypeptidase activity trans-Golgi network YLR021W YLR021W IRC25 hom Verified 0.546454049778324 0.292376932672263 no 1372 0.298330547647706 996 NA FT PROTEIN COMPLEX ORG/BIOGENESIS Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions proteasome assembly|chaperone-mediated protein complex assembly|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function intracellular YER119C-A_d YER119C-A YER119C-A hom Dubious 0.54579266704286 0.292604232231082 no 1373 0.322765668220664 1118 NA FF Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Unknown Unknown Unknown YKL087C YKL087C CYT2 hom Verified 0.545154887431527 0.292823497752272 no 1374 0.293073855626028 1020 NA FT PROTEIN COMPLEX ORG/BIOGENESIS MITOCHONDRION Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 cytochrome c-heme linkage holocytochrome-c synthase activity mitochondrion|mitochondrial intermembrane space YMR159C YMR159C ATG16 hom Verified 0.54505882786105 0.292856529172884 no 1375 0.292177083500408 1016 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress C-terminal protein lipidation|piecemeal microautophagy of nucleus|mitochondrion degradation|macroautophagy|CVT pathway protein binding, bridging|Atg8 ligase activity Atg12-Atg5-Atg16 complex|pre-autophagosomal structure YLR357W YLR357W RSC2 hom Verified 0.544402275190598 0.293082340245318 no 1376 0.301612985362437 1019 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance ATP-dependent chromatin remodeling|chromosome segregation|nucleosome disassembly|plasmid maintenance|sister chromatid cohesion|double-strand break repair via nonhomologous end joining|transcription elongation from RNA polymerase II promoter|double-strand break repair via homologous recombination DNA translocase activity RSC complex YNL104C YNL104C LEU4 hom Verified 0.544300774580194 0.293117257006865 no 1377 0.267255212410553 906 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication leucine biosynthetic process 2-isopropylmalate synthase activity mitochondrion|cytoplasm YFR038W YFR038W IRC5 hom Verified 0.543883417002878 0.293260850552856 no 1378 0.282421666158416 976 NA FT NUCLEUS Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci mitotic recombination molecular_function cellular_component YAR015W YAR015W ADE1 hom Verified 0.542925119249066 0.29359068006066 no 1379 0.290748048854477 982 NA FT NUCLEOTIDE METABOLISM NUCLEUS N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process phosphoribosylaminoimidazolesuccinocarboxamide synthase activity nucleus|cytoplasm YKR094C YKR094C RPL40B hom Verified 0.542431158491367 0.293760759863368 no 1380 0.284372619593464 977 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION NUCLEUS|MITOCHONDRION RIBOSOME Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication cytoplasmic translation|protein ubiquitination|ribosome biogenesis structural constituent of ribosome|protein tag mitochondrion|cytosolic large ribosomal subunit YOL090W YOL090W MSH2 hom Verified 0.542399731181106 0.293771582408579 no 1381 0.302698905096353 1040 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP mismatch repair|mitotic recombination|meiotic gene conversion|meiotic mismatch repair|removal of nonhomologous ends|DNA recombination|chromatin silencing at silent mating-type cassette loop DNA binding|single base insertion or deletion binding|ATP binding|Y-form DNA binding|double-strand/single-strand DNA junction binding|DNA insertion or deletion binding|guanine/thymine mispair binding|four-way junction DNA binding|ATPase activity MutSbeta complex|nuclear chromosome|MutSalpha complex|nucleus YLR286C YLR286C CTS1 hom Verified 0.542065525997237 0.293886683233083 no 1382 0.275997648129963 978 NA FT CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p cytokinesis, completion of separation|chitin catabolic process endochitinase activity nuclear envelope|extracellular region|fungal-type cell wall|endoplasmic reticulum|cellular bud neck YMR081C YMR081C ISF1 hom Verified 0.541287042321551 0.294154875160872 no 1383 0.281479356820247 976 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant aerobic respiration molecular_function cellular_component YHR009C_p YHR009C TDA3 hom Uncharacterized 0.541070325955871 0.294229555264724 no 1384 0.300228655554212 1033 NA FT VESICLE-MEDIATED TRANSPORT|OXIDATION-REDUCTION PROCESS Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 retrograde transport, endosome to Golgi molecular_function late endosome|cytoplasm YMR095C YMR095C SNO1 hom Verified 0.5402781749712 0.294502603683222 no 1385 0.267573121980267 888 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase pyridoxine metabolic process|thiamine biosynthetic process|anti-apoptosis molecular_function cytoplasm YMR078C YMR078C CTF18 hom Verified 0.539794428952601 0.294669404747454 no 1386 0.268718698357994 923 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CHROMOSOME Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint maintenance of DNA trinucleotide repeats|double-strand break repair via homologous recombination|DNA replication initiation|mitotic sister chromatid cohesion molecular_function mitochondrion|nuclear replication fork|Ctf18 RFC-like complex YLR405W YLR405W DUS4 hom Verified 0.538937012494096 0.294965158592726 no 1387 0.284528050099628 956 NA FT RNA PROCESSING|OXIDATION-REDUCTION PROCESS Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p tRNA modification tRNA dihydrouridine synthase activity cellular_component YKR084C YKR084C HBS1 hom Verified 0.538129378157364 0.29524386581514 no 1388 0.302699810699198 1022 NA FT TRANSLATION|NUCLEOTIDE METABOLISM GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay nuclear-transcribed mRNA catabolic process, no-go decay|ribosome disassembly|nonfunctional rRNA decay ribosome binding|GTPase activity cytoplasm YLL007C_p YLL007C YLL007C hom Uncharacterized 0.537746794051464 0.295375934388655 no 1389 0.310042618604764 1094 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene biological_process molecular_function cytoplasm YJL075C_d YJL075C APQ13 hom Dubious 0.537687203108093 0.295396507712694 no 1390 0.296941094102494 1005 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Unknown Unknown Unknown YFR049W YFR049W YMR31 hom Verified 0.537378829819529 0.295502981797146 no 1391 0.318893337573537 1077 NA FT TRANSLATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 cytoplasmic translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YOR320C YOR320C GNT1 hom Verified 0.537239982407516 0.295550928322854 no 1392 0.293800997464115 982 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus protein N-linked glycosylation acetylglucosaminyltransferase activity Golgi medial cisterna YLR025W YLR025W SNF7 hom Verified 0.537174704801942 0.295573471098233 no 1393 0.354157743901228 1201 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes cellular response to anoxia|intralumenal vesicle formation|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|late endosome to vacuole transport protein binding ESCRT III complex|cytoplasm YKL184W YKL184W SPE1 hom Verified 0.53416907078257 0.296612282884294 no 1394 0.298326938964256 1031 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation pantothenate biosynthetic process|putrescine biosynthetic process ornithine decarboxylase activity cytoplasm YBR085C-A YBR085C-A YBR085C-A hom Verified 0.533598952477903 0.296809516576297 no 1395 0.27949539006619 985 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YBR056W YBR056W YBR056W hom Verified 0.53009480130587 0.298023101667861 no 1396 0.281463690233212 969 NA FT CARBOHYDRATE METABOLISM Putative glycoside hydrolase of the mitochondrial intermembrane space biological_process molecular_function cytoplasm YMR017W YMR017W SPO20 hom Verified 0.528286663803031 0.298650193218357 no 1397 0.271203924852184 936 NA FT PLASMA MEMBRANE Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog ascospore-type prospore assembly SNAP receptor activity SNARE complex|prospore membrane YLL020C_d YLL020C YLL020C hom Dubious 0.528159577457625 0.298694291392294 no 1398 0.276940522383144 919 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Unknown Unknown Unknown YJR034W YJR034W PET191 hom Verified 0.527952714513001 0.298766077881775 no 1399 0.308808677939681 1041 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion|integral to mitochondrial inner membrane YDL136W YDL136W RPL35B hom Verified 0.52793303917957 0.298772906110943 no 1400 0.294480475770816 983 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication cytoplasmic translation|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic large ribosomal subunit|preribosome, large subunit precursor YGR042W YGR042W YGR042W hom Verified 0.52680555453521 0.299164312623131 no 1401 0.279008127731204 968 NA FT NUCLEUS Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress biological_process molecular_function nucleus|cytoplasm YOL032W YOL032W OPI10 hom Verified 0.526787975656898 0.299170416976211 no 1402 0.264241590322757 906 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM NUCLEUS Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress inositol metabolic process molecular_function nucleus|cytoplasm YJL036W YJL036W SNX4 hom Verified 0.526672327193382 0.299210577890575 no 1403 0.333365889339678 1127 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p piecemeal microautophagy of nucleus|mitochondrion degradation|early endosome to Golgi transport|CVT pathway|protein targeting to vacuole phosphatidylinositol-3-phosphate binding cytosol|pre-autophagosomal structure|extrinsic to membrane|early endosome YHR025W YHR025W THR1 hom Verified 0.525503478531603 0.299616617891174 no 1404 0.319469062351259 1088 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway threonine biosynthetic process|methionine metabolic process|isoleucine metabolic process|homoserine metabolic process homoserine kinase activity cellular_component YLR225C_p YLR225C YLR225C hom Uncharacterized 0.525387434727916 0.299656943339439 no 1405 0.305725089839801 1050 NA FT RESPONSE TO OXIDATIVE STRESS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene biological_process molecular_function cytoplasm YOR202W YOR202W HIS3 hom Verified 0.524790188227255 0.299864526545667 no 1406 0.274140719841409 952 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p histidine biosynthetic process imidazoleglycerol-phosphate dehydratase activity cellular_component YLR064W YLR064W PER33 hom Verified 0.524372886926099 0.300009605349917 no 1407 0.292188767697681 999 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM NUCLEAR PORE Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress biological_process molecular_function integral to membrane|endoplasmic reticulum|nuclear pore YDR278C_d YDR278C YDR278C hom Dubious 0.523868321955049 0.300185064608242 no 1408 0.289299287582625 989 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR224W_p YLR224W YLR224W hom Uncharacterized 0.523664678241929 0.300255893553951 no 1409 0.282705233801313 946 NA FT PROTEOLYSIS F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex YHL002W YHL002W HSE1 hom Verified 0.52226806286024 0.300741851213291 no 1410 0.310391437039786 1045 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes late endosome to vacuole transport|protein targeting to vacuole ubiquitin binding endosome|ESCRT-0 complex YML013W YML013W UBX2 hom Verified 0.521721897811115 0.300931987914945 no 1411 0.361355092126455 1235 NA FF|FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION UBIQUITIN LIGASE COMPLEX Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis proteasomal ubiquitin-dependent protein catabolic process|ER-associated protein catabolic process|lipid particle organization protein binding, bridging mitochondrial outer membrane|Doa10p ubiquitin ligase complex|integral to endoplasmic reticulum membrane|lipid particle|endoplasmic reticulum YPR106W YPR106W ISR1 hom Verified 0.521317043541872 0.30107296499946 no 1412 0.28981992995337 974 NA FT PROTEIN PHOSPHORYLATION Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C biological_process protein kinase activity cellular_component YKL160W YKL160W ELF1 hom Verified 0.521314985965836 0.301073681558153 no 1413 0.288395264635384 967 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression regulation of transcription by chromatin organization|transcription elongation from RNA polymerase II promoter RNA polymerase II core binding transcription elongation factor complex|nucleus YPL144W YPL144W POC4 hom Verified 0.521048269933752 0.301166572938951 no 1414 0.290473586186911 999 NA FT PROTEIN COMPLEX ORG/BIOGENESIS Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions proteasome assembly|chaperone-mediated protein complex assembly molecular_function intracellular|cytoplasm YNL022C_p YNL022C YNL022C hom Uncharacterized 0.517931688325282 0.302252965917715 no 1415 0.288439145151834 1021 NA FT NUCLEUS Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis biological_process tRNA (cytosine-5-)-methyltransferase activity nucleus YLR324W YLR324W PEX30 hom Verified 0.51755894449708 0.302383016406751 no 1416 0.328898111982525 1133 NA FF|FT PEROXISOME ORGANIZATION Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p peroxisome organization molecular_function integral to membrane|peroxisome|integral to peroxisomal membrane YJL020C YJL020C BBC1 hom Verified 0.515903178541465 0.302961016992398 no 1417 0.279811263811518 954 NA FT CYTOSKELETON ORGANIZATION CYTOSKELETON Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches actin cytoskeleton organization myosin I binding actin cortical patch YLR439W YLR439W MRPL4 hom Verified 0.515747747997037 0.303015300610228 no 1418 0.337600030505056 1177 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YMR011W YMR011W HXT2 hom Verified 0.514514677109317 0.303446100776292 no 1419 0.301777509411012 1033 NA FT PLASMA MEMBRANE High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose glucose transport pentose transmembrane transporter activity|mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity integral to membrane|plasma membrane YLR204W YLR204W QRI5 hom Verified 0.513868534460514 0.303671953980257 no 1420 0.343426957274797 1169 NA FF|FT RNA PROCESSING MITOCHONDRION Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA mRNA processing molecular_function mitochondrion|integral to mitochondrial inner membrane YPL033C YPL033C SRL4 hom Verified 0.513410655520526 0.303832046751841 no 1421 0.301152674395994 1013 NA FT NUCLEOTIDE METABOLISM Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p deoxyribonucleoside triphosphate metabolic process molecular_function cellular_component YHR161C YHR161C YAP1801 hom Verified 0.512443025246056 0.30417049274521 no 1422 0.305553714709007 1047 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication endocytosis clathrin binding actin cortical patch YPL227C YPL227C ALG5 hom Verified 0.512376566894971 0.304193743905113 no 1423 0.310431958548982 1055 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum protein N-linked glycosylation dolichyl-phosphate beta-glucosyltransferase activity endoplasmic reticulum membrane YIL002C YIL002C INP51 hom Verified 0.511656116262491 0.30444585200882 no 1424 0.338980632252118 1143 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|MEMBRANE ORGANIZATION CYTOSKELETON Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth phosphatidylinositol dephosphorylation phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity membrane|cytoplasm YPL041C_p YPL041C YPL041C hom Uncharacterized 0.509581664454287 0.305172286070734 no 1425 0.296678545554735 990 NA Protein of unknown function involved in maintenance of proper telomere length biological_process molecular_function cellular_component YIL124W YIL124W AYR1 hom Verified 0.508145073148206 0.305675803776495 no 1426 0.306912494211401 1036 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOPLASMIC RETICULUM|MITOCHONDRION NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones phosphatidic acid biosynthetic process acylglycerone-phosphate reductase activity mitochondrial outer membrane|mitochondrion|integral to membrane|endoplasmic reticulum|lipid particle|cytoplasm YMR175W-A_p YMR175W-A YMR175W-A hom Uncharacterized 0.507711281356811 0.305827917834341 no 1427 0.285908527089227 975 NA Putative protein of unknown function biological_process molecular_function cellular_component YER130C_p YER130C YER130C hom Uncharacterized 0.505555911159435 0.306584219477579 no 1428 0.358254084736778 1185 NA FT NUCLEUS Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress biological_process sequence-specific DNA binding cellular_component YDR483W YDR483W KRE2 hom Verified 0.505403935579906 0.306637577606541 no 1429 0.346164555188046 1141 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication N-glycan processing|cell wall mannoprotein biosynthetic process|protein O-linked glycosylation alpha-1,2-mannosyltransferase activity Golgi apparatus YGR058W YGR058W PEF1 hom Verified 0.502647869319588 0.307605933665922 no 1430 0.296546227711124 1033 NA FT CELL CYCLE|CELL DIVISION NUCLEUS|SITE OF POLARIZED GROWTH Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly bipolar cellular bud site selection|regulation of COPII vesicle coating|axial cellular bud site selection zinc ion binding|calcium ion binding cytosol|site of polarized growth|nucleus|cellular bud neck|cytoplasm YOR002W YOR002W ALG6 hom Verified 0.502620801647763 0.30761545067782 no 1431 0.352692009599582 1218 NA FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease oligosaccharide-lipid intermediate biosynthetic process|protein glycosylation|aerobic respiration transferase activity, transferring hexosyl groups|dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity integral to membrane|endoplasmic reticulum YNL049C YNL049C SFB2 hom Verified 0.502530416590373 0.307647231063739 no 1432 0.294770650638934 982 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat YNL031C YNL031C HHT2 hom Verified 0.501939026184619 0.307855206085034 no 1433 0.293620589875324 982 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation chromatin assembly or disassembly|global genome nucleotide-excision repair|rRNA transcription|sexual sporulation resulting in formation of a cellular spore|mitotic cell cycle spindle assembly checkpoint DNA binding nuclear nucleosome YKR058W YKR058W GLG1 hom Verified 0.501030488110906 0.308174833105677 no 1434 0.298927394960893 1043 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication glycogen biosynthetic process glycogenin glucosyltransferase activity cellular_component YER005W YER005W YND1 hom Verified 0.500587311403925 0.30833079711345 no 1435 0.304167882207894 1049 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM GOLGI APPARATUS Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity protein glycosylation nucleoside-triphosphatase activity|nucleoside-diphosphatase activity COPI-coated vesicle|Golgi apparatus|membrane|Golgi membrane YNL215W YNL215W IES2 hom Verified 0.500282714816109 0.308438011677807 no 1436 0.320159545492782 1072 NA FT NUCLEUS Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YDR312W YDR312W SSF2 hom Verified 0.499634782929233 0.308666130731129 no 1437 0.273713597439444 916 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication conjugation with cellular fusion|ribosomal large subunit assembly rRNA binding nucleolus YPR084W_p YPR084W YPR084W hom Uncharacterized 0.498810123066012 0.308956577677531 no 1438 0.312625069299013 1055 NA Putative protein of unknown function biological_process molecular_function cellular_component YLR306W YLR306W UBC12 hom Verified 0.49879594964112 0.308961570632722 no 1439 0.295997593887633 1012 NA Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes protein neddylation NEDD8 ligase activity cellular_component YML024W YML024W RPS17A hom Verified 0.498715033912945 0.308990075964978 no 1440 0.293267747696093 1022 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YDR119W_p YDR119W VBA4 hom Uncharacterized 0.49870462594667 0.308993742610511 no 1441 0.279031451396752 947 NA Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene biological_process molecular_function fungal-type vacuole membrane|integral to membrane YPL152W-A_p YPL152W-A YPL152W-A hom Uncharacterized 0.498018895478459 0.309235362038535 no 1442 0.299303378006043 1037 NA Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YNL219C YNL219C ALG9 hom Verified 0.49712851119159 0.309549215062365 no 1443 0.321251292135124 1063 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation mannosyltransferase activity integral to membrane|endoplasmic reticulum YKR073C_d YKR073C YKR073C hom Dubious 0.49707166844168 0.309569256377487 no 1444 0.340881156205619 1147 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR349W_d YLR349W YLR349W hom Dubious 0.49651418691929 0.309765840257326 no 1445 0.303470403845406 1019 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Unknown Unknown Unknown YDR446W YDR446W ECM11 hom Verified 0.496008028409792 0.309944373317543 no 1446 0.274065957311823 911 NA FT CELL CYCLE|CELL WALL ORG/BIOGENESIS NUCLEUS Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure fungal-type cell wall organization|reciprocal meiotic recombination molecular_function nucleus YER174C YER174C GRX4 hom Verified 0.495013808597281 0.310295186634725 no 1447 0.311056561752431 1056 NA FT ION HOMEOSTASIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS NUCLEUS Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 cellular iron ion homeostasis|cellular response to oxidative stress|actin cytoskeleton organization disulfide oxidoreductase activity nucleus YOL045W YOL045W PSK2 hom Verified 0.494878973725952 0.31034277681083 no 1448 0.326284786880101 1062 NA FT SIGNALING|TRANSLATION|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication regulation of (1->6)-beta-D-glucan biosynthetic process|negative regulation of glycogen biosynthetic process|protein phosphorylation protein serine/threonine kinase activity mitochondrion|cytoplasm YJL124C YJL124C LSM1 hom Verified 0.494540563870052 0.310462233077825 no 1449 0.331183963976232 1126 NA FF|FT RNA PROCESSING NUCLEUS Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay RNA binding|RNA cap binding mRNA cap binding complex|cytoplasmic mRNA processing body|cytoplasm YKL020C YKL020C SPT23 hom Verified 0.494522117913781 0.310468744943606 no 1450 0.298460370295383 1041 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting cellular response to cold|positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA binding integral to endoplasmic reticulum membrane|nucleus YNL121C YNL121C TOM70 hom Verified 0.493657195536313 0.31077414998943 no 1451 0.345258765653918 1163 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication protein import into mitochondrial matrix|protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YGL014W YGL014W PUF4 hom Verified 0.492974908193652 0.311015158436093 no 1452 0.332945279719843 1137 NA FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|TRANSLATION|GENE SILENCING Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|negative regulation of translation|protein localization|negative regulation of chromatin silencing involved in replicative cell aging mRNA binding cytoplasm YAL040C YAL040C CLN3 hom Verified 0.491698583362165 0.311466219930476 no 1453 0.309737199618265 1071 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis vacuole organization|regulation of cyclin-dependent protein kinase activity|regulation of transcription involved in G1/S phase of mitotic cell cycle|vacuole fusion, non-autophagic|regulation of cell size|G1/S transition of mitotic cell cycle cyclin-dependent protein kinase regulator activity nucleus YBR258C YBR258C SHG1 hom Verified 0.491214085084281 0.311637518948369 no 1454 0.303720842253619 1000 NA FT CHROMATIN ORGANIZATION NUCLEUS Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres histone H3-K4 methylation histone methyltransferase activity (H3-K4 specific) nucleus|Set1C/COMPASS complex YMR297W YMR297W PRC1 hom Verified 0.490516282113197 0.311884305530627 no 1455 0.277531996186813 950 NA FT PROTEOLYSIS ENDOPLASMIC RETICULUM Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family vacuolar protein catabolic process|phytochelatin biosynthetic process serine-type carboxypeptidase activity fungal-type vacuole lumen|endoplasmic reticulum|fungal-type vacuole|cytoplasm YDR317W YDR317W HIM1 hom Verified 0.490127082235472 0.31202198755044 no 1456 0.293112240703276 990 NA FT RESPONSE TO DNA DAMAGE Protein of unknown function involved in DNA repair DNA repair molecular_function cellular_component YGR081C YGR081C SLX9 hom Verified 0.487907818892764 0.312807567998928 no 1457 0.32027180558877 1089 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YBR036C YBR036C CSG2 hom Verified 0.487397735686204 0.312988248937504 no 1458 0.333847876225567 1118 NA FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress cellular calcium ion homeostasis|glycosphingolipid biosynthetic process enzyme regulator activity integral to endoplasmic reticulum membrane|integral to membrane YER039C-A_p YER039C-A YER039C-A hom Uncharacterized 0.48709972409361 0.313093830967749 no 1459 0.310492086183416 1030 NA Putative protein of unknown function; YER039C-A is not an essential gene biological_process molecular_function integral to membrane YCR063W YCR063W BUD31 hom Verified 0.484387540326967 0.314055429658093 no 1460 0.285885673367658 973 NA FT CELL CYCLE|RNA PROCESSING|CELL DIVISION NUCLEUS Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis RNA splicing|mRNA splicing, via spliceosome|cellular bud site selection molecular_function U2 snRNP|nucleus YIR018W YIR018W YAP5 hom Verified 0.483903578635506 0.314227150260538 no 1461 0.304202176139007 1050 NA FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING NUCLEUS|CHROMOSOME Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication negative regulation of chromatin silencing at telomere|negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to iron|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding RNA polymerase II transcription factor activity nuclear chromatin|nucleus YGR131W YGR131W FHN1 hom Verified 0.483648827491163 0.314317557913595 no 1462 0.322850195000664 1080 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION PLASMA MEMBRANE Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain establishment of protein localization to plasma membrane molecular_function integral to membrane|membrane raft YPL135W YPL135W ISU1 hom Verified 0.482643783122295 0.314674342865981 no 1463 0.321582715332878 1076 NA FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS|COFACTOR METABOLISM MITOCHONDRION Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable cellular iron ion homeostasis|iron-sulfur cluster assembly|tRNA wobble uridine modification ferrous iron binding mitochondrion|mitochondrial matrix YHR157W YHR157W REC104 hom Verified 0.479714831309806 0.315715090199598 no 1464 0.301689617543745 1043 NA FT CELL CYCLE NUCLEUS|CHROMOSOME Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination meiotic DNA double-strand break formation|reciprocal meiotic recombination molecular_function condensed nuclear chromosome YGR097W YGR097W ASK10 hom Verified 0.477802060961345 0.316395547068636 no 1465 0.322938670094805 1107 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO OXIDATIVE STRESS NUCLEUS RNA POL II, HOLOENZYME Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication cellular protein catabolic process|cellular response to oxidative stress|positive regulation of transcription from RNA polymerase II promoter|positive regulation of glycerol transport molecular_function DNA-directed RNA polymerase II, holoenzyme|cytoplasm YPL060W YPL060W MFM1 hom Verified 0.477422809257348 0.316530537615719 no 1466 0.338506298619508 1114 NA FT RNA PROCESSING|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p mitochondrial magnesium ion transport magnesium ion transmembrane transporter activity integral to membrane|mitochondrial inner membrane YHL027W YHL027W RIM101 hom Verified 0.477241869048038 0.316594949942609 no 1467 0.343840968648484 1179 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|ION HOMEOSTASIS|CELL WALL ORG/BIOGENESIS|CELL DIVISION|ION HOMEOSTASIS NUCLEUS Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC meiosis|negative regulation of transcription from RNA polymerase II promoter|fungal-type cell wall biogenesis|cellular response to anoxia|response to pH|ascospore formation|barrier septum assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|sequence-specific DNA binding nucleus YFR001W YFR001W LOC1 hom Verified 0.476039407453547 0.317023151637634 no 1468 0.34210188576219 1153 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION NUCLEUS Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|intracellular mRNA localization mRNA binding nucleolus|preribosome, large subunit precursor YAL015C YAL015C NTG1 hom Verified 0.475382171708617 0.317257299744303 no 1469 0.301115716238786 1024 NA FT RESPONSE TO DNA DAMAGE|MITOCHONDRION ORGANIZATION|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication DNA repair|cellular response to oxidative stress|base-excision repair|base-excision repair, AP site formation|positive regulation of mitochondrial DNA replication oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity|oxidized purine nucleobase lesion DNA N-glycosylase activity|DNA-(apurinic or apyrimidinic site) lyase activity mitochondrion|nucleus YDR197W YDR197W CBS2 hom Verified 0.474686707197021 0.317505146981214 no 1470 0.33780973703628 1145 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader mitochondrial respiratory chain complex III biogenesis|positive regulation of mitochondrial translation translation regulator activity mitochondrion|extrinsic to mitochondrial inner membrane|mitochondrial ribosome YBR105C YBR105C VID24 hom Verified 0.473868421325596 0.317796869651834 no 1471 0.327290749369927 1118 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM|PROTEOLYSIS|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles negative regulation of gluconeogenesis|vesicle fusion with vacuole|vacuolar protein catabolic process|proteasomal ubiquitin-dependent protein catabolic process|vesicle-mediated transport|protein targeting to vacuole molecular_function cytoplasmic membrane-bounded vesicle|GID complex|extrinsic to membrane YGL231C YGL231C EMC4 hom Verified 0.473865494471724 0.317797913291834 no 1472 0.336812556192911 1145 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|endoplasmic reticulum YCR068W YCR068W ATG15 hom Verified 0.473561726228624 0.317906237019706 no 1473 0.337096225617401 1130 NA FT LIPID METABOLISM|MEMBRANE ORGANIZATION|VACUOLAR PROTEIN PROCESSING ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway membrane disassembly|autophagy|piecemeal microautophagy of nucleus|vacuolar protein processing|multivesicular body membrane disassembly lipase activity integral to membrane|vacuolar lumen YDR518W YDR518W EUG1 hom Verified 0.473354339526091 0.317980200050878 no 1474 0.309796970535973 1049 NA FT ENDOPLASMIC RETICULUM Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER protein folding protein disulfide isomerase activity|protein disulfide oxidoreductase activity endoplasmic reticulum YPL004C YPL004C LSP1 hom Verified 0.473208742517252 0.318032130554165 no 1475 0.306848835175162 1032 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION MITOCHONDRION Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family negative regulation of protein kinase activity|endocytosis|response to heat lipid binding mitochondrial outer membrane|eisosome|mitochondrion|plasma membrane|cytoplasm YBR196C-A_p YBR196C-A YBR196C-A hom Uncharacterized 0.472110850064625 0.318423834163482 no 1476 0.352494916989279 1169 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YNL041C YNL041C COG6 hom Verified 0.47179962638529 0.318534908810398 no 1477 0.409577822184735 1350 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments intra-Golgi vesicle-mediated transport|CVT pathway molecular_function Golgi transport complex YMR086W YMR086W SEG1 hom Verified 0.47117187073972 0.318759002235178 no 1478 0.314931132386638 1094 NA Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes biological_process molecular_function eisosome|ribosome|cytoplasm YDR184C YDR184C ATC1 hom Verified 0.470581413358039 0.318969841593645 no 1479 0.338105899943923 1131 NA FT CELL CYCLE|CELL DIVISION NUCLEUS Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress bipolar cellular bud site selection|response to stress molecular_function nucleus YDR017C YDR017C KCS1 hom Verified 0.470483239254984 0.319004903091306 no 1480 0.285529196095499 966 NA Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance inositol phosphate biosynthetic process inositol heptakisphosphate kinase activity|inositol hexakisphosphate kinase activity|inositol-1,3,4,5,6-pentakisphosphate kinase activity|inositol heptakisphosphate 5-kinase activity cytoplasm YJR090C YJR090C GRR1 hom Verified 0.470272964794347 0.319080005101007 no 1481 0.354090007575576 1184 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|PROTEOLYSIS NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON UBIQUITIN LIGASE COMPLEX F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury|cell cycle arrest in response to pheromone|G1/S transition of mitotic cell cycle|response to DNA damage stimulus|protein ubiquitination ubiquitin-protein ligase activity|protein binding, bridging cellular bud neck contractile ring|SCF ubiquitin ligase complex|nucleus|cytoplasm YGL154C YGL154C LYS5 hom Verified 0.469450049053876 0.319373990570152 no 1482 0.299404627401574 1024 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine lysine biosynthetic process via aminoadipic acid|protein-cofactor linkage holo-[acyl-carrier-protein] synthase activity cytoplasm YDR100W YDR100W TVP15 hom Verified 0.46857506236209 0.319686702911288 no 1483 0.324056322799966 1086 NA FT VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p vesicle-mediated transport molecular_function COPI-coated vesicle|integral to membrane|integral to Golgi membrane YEL020C_p YEL020C YEL020C hom Uncharacterized 0.467077529321717 0.320222205147439 no 1484 0.325937076071902 1096 NA Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data biological_process molecular_function cytoplasm YOR311C YOR311C DGK1 hom Verified 0.465709545203169 0.320711709536745 no 1485 0.301164043985303 1033 NA FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|LIPID METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain phosphatidic acid biosynthetic process diacylglycerol kinase activity integral to endoplasmic reticulum membrane|integral to membrane YIL024C_p YIL024C YIL024C hom Uncharacterized 0.465275281008912 0.320867167126345 no 1486 0.316189514506761 1082 NA Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p biological_process molecular_function cellular_component YMR251W YMR251W GTO3 hom Verified 0.464607945927706 0.321106120487357 no 1487 0.332507620518142 1151 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Omega class glutathione transferase; putative cytosolic localization glutathione metabolic process glutathione transferase activity cytoplasm YHL037C_d YHL037C YHL037C hom Dubious 0.463346349901093 0.321558063866237 no 1488 0.308291854385299 1003 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR239C YDR239C YDR239C hom Verified 0.463043942851853 0.321666434868563 no 1489 0.324169483951216 1101 NA Protein of unknown function that may interact with ribosomes, based on co-purification experiments biological_process molecular_function ribosome|cytoplasm YDR338C_p YDR338C YDR338C hom Uncharacterized 0.462914374936768 0.321712871647741 no 1490 0.344339930073225 1166 NA Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily transmembrane transport antiporter activity integral to membrane YER075C YER075C PTP3 hom Verified 0.462831268320273 0.321742658293425 no 1491 0.306299762720258 1048 NA FT PROTEIN LOCALIZATION|SIGNALING|PROTEIN PHOSPHORYLATION NUCLEUS Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm inactivation of MAPK activity involved in osmosensory signaling pathway|regulation of sporulation|regulation of protein localization|protein dephosphorylation|inactivation of MAPK activity involved in conjugation with cellular fusion protein tyrosine phosphatase activity nucleus|cytoplasm YMR275C YMR275C BUL1 hom Verified 0.462430485294454 0.321886320965371 no 1492 0.333728611125581 1160 NA FT MITOCHONDRION ORGANIZATION PLASMA MEMBRANE UBIQUITIN LIGASE COMPLEX Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication mitochondrion inheritance|protein monoubiquitination|protein polyubiquitination ubiquitin-ubiquitin ligase activity ubiquitin ligase complex|plasma membrane YOR290C YOR290C SNF2 hom Verified 0.461565304297251 0.322196540165635 no 1493 0.384419126456244 1267 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS NUCLEUS RSC COMPLEX Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p cellular alcohol catabolic process|ATP-dependent chromatin remodeling|double-strand break repair|strand invasion|positive regulation of mating type switching|sucrose catabolic process|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|nucleosome mobilization|chromatin remodeling|DNA-dependent DNA replication|positive regulation of transcription from RNA polymerase II promoter histone acetyl-lysine binding|RNA polymerase II activating transcription factor binding|DNA-dependent ATPase activity|nucleosomal DNA binding SWI/SNF complex|nucleus YKL062W YKL062W MSN4 hom Verified 0.461302444907152 0.322290815548568 no 1494 0.323404922407413 1078 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS NUCLEUS Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|cellular response to acidity|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|replicative cell aging|age-dependent response to oxidative stress involved in chronological cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YOL164W YOL164W BDS1 hom Verified 0.460911541256119 0.322431035566417 no 1495 0.337507910125404 1116 NA Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources dodecyl sulfate metabolic process arylsulfatase activity|alkyl sulfatase activity cellular_component YCR053W YCR053W THR4 hom Verified 0.460723155622795 0.32249861990337 no 1496 0.302286467850191 1011 NA FT VESICLE-MEDIATED TRANSPORT|KETONE METABOLISM|MEMBRANE ORGANIZATION|AMINO ACID METABOLISM NUCLEUS Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway endocytosis|threonine metabolic process threonine synthase activity nucleus|cytoplasm YLR173W_p YLR173W YLR173W hom Uncharacterized 0.460634978285915 0.322530256002423 no 1497 0.326592625594566 1096 NA Putative protein of unknown function biological_process molecular_function cellular_component YBR071W YBR071W YBR071W hom Verified 0.460491165859291 0.322581855511169 no 1498 0.319477640615277 1050 NA FT SITE OF POLARIZED GROWTH Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking biological_process molecular_function cellular bud neck|cytoplasm YGR026W_p YGR026W YGR026W hom Uncharacterized 0.460323668142761 0.322641957545471 no 1499 0.333114124045932 1128 NA FT PLASMA MEMBRANE Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function integral to membrane YLR055C YLR055C SPT8 hom Verified 0.459788917900856 0.322833869243734 no 1500 0.331753644612549 1128 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters histone acetylation|negative regulation of transcription from RNA polymerase II promoter|chromatin modification|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|transcription cofactor activity nucleus|SAGA complex YOR047C YOR047C STD1 hom Verified 0.458863110400036 0.323166235511017 no 1501 0.33247980769372 1131 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING|CARBOHYDRATE METABOLISM NUCLEUS|PLASMA MEMBRANE Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] response to salt stress|signal transduction|nicotinamide riboside biosynthetic process|glucose metabolic process|nicotinic acid riboside biosynthetic process|regulation of transcription from RNA polymerase II promoter protein kinase activator activity plasma membrane|nucleus YGR100W YGR100W MDR1 hom Verified 0.458429697961381 0.323321879766168 no 1502 0.329053878142081 1126 NA FT PROTEIN LOCALIZATION|SIGNALING|NUCLEOTIDE METABOLISM Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function intracellular protein transport Rab GTPase activator activity cytoplasm YLR343W YLR343W GAS2 hom Verified 0.457352284503561 0.323708927430784 no 1503 0.283095951868308 952 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM PLASMA MEMBRANE 1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p ascospore wall assembly 1,3-beta-glucanosyltransferase activity integral to membrane|cytoplasm YHR203C YHR203C RPS4B hom Verified 0.456813151346782 0.32390267608771 no 1504 0.347265168193216 1167 NA FT TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YBR047W_p YBR047W FMP23 hom Uncharacterized 0.45671277035804 0.323938755344623 no 1505 0.319518597381768 1102 NA FT MITOCHONDRION Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YGR231C YGR231C PHB2 hom Verified 0.456645435037949 0.323962958148274 no 1506 0.33262214462399 1145 NA FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation mitochondrion inheritance|protein folding|negative regulation of proteolysis|replicative cell aging molecular_function mitochondrion|mitochondrial inner membrane YER124C YER124C DSE1 hom Verified 0.456182069291374 0.324129529106602 no 1507 0.32676182925885 1098 NA FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION SITE OF POLARIZED GROWTH Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress cytokinesis, completion of separation|regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion|invasive growth in response to glucose limitation molecular_function cellular bud neck YKR010C YKR010C TOF2 hom Verified 0.456155530322874 0.324139070415166 no 1508 0.31017830547463 1064 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION|GENE SILENCING NUCLEUS|MITOCHONDRION Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication nucleolus organization|rDNA condensation|chromatin silencing at rDNA|protein localization to nucleolar rDNA repeats phosphatase activator activity mitochondrion|nucleolus YDL216C YDL216C RRI1 hom Verified 0.452538727766689 0.325440464081392 no 1509 0.326199737560196 1128 NA FT SIGNALING|PROTEOLYSIS NUCLEUS Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion|cullin deneddylation metalloendopeptidase activity signalosome YML089C_d YML089C YML089C hom Dubious 0.451621708802859 0.325770764080393 no 1510 0.309315797339771 1000 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Unknown Unknown Unknown YPR037C YPR037C ERV2 hom Verified 0.450800678574595 0.326066606102095 no 1511 0.324407162478776 1100 NA FT OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER protein thiol-disulfide exchange thiol oxidase activity integral to endoplasmic reticulum membrane YNL288W YNL288W CAF40 hom Verified 0.450416456460748 0.326205090577468 no 1512 0.32728254027737 1098 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter molecular_function CCR4-NOT core complex YBL003C YBL003C HTA2 hom Verified 0.449530170067532 0.326524624547501 no 1513 0.321149967302764 1096 NA FT CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p DNA repair|chromatin assembly or disassembly DNA binding replication fork protection complex|nuclear nucleosome YLR322W_d YLR322W VPS65 hom Dubious 0.449019101825911 0.326708938531005 no 1514 0.332071398060957 1126 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Unknown Unknown Unknown YGL190C YGL190C CDC55 hom Verified 0.448553364548121 0.326876940989232 no 1515 0.433000460548535 1433 NA FT CELL CYCLE|SIGNALING NUCLEUS|SITE OF POLARIZED GROWTH Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion negative regulation of exit from mitosis|positive regulation of G2/M transition of mitotic cell cycle|cell shape checkpoint|protein dephosphorylation protein phosphatase type 2A regulator activity nucleus|cellular bud neck|cytoplasm|cellular bud tip YNR063W_p YNR063W YNR063W hom Uncharacterized 0.448489405668814 0.326900015212445 no 1516 0.312085900096273 1068 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Putative zinc-cluster protein of unknown function biological_process sequence-specific DNA binding cellular_component YLR130C YLR130C ZRT2 hom Verified 0.448367296987867 0.326944069776103 no 1517 0.32505882092757 1048 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor low-affinity zinc ion transport low-affinity zinc ion transmembrane transporter activity integral to membrane|plasma membrane YGL229C YGL229C SAP4 hom Verified 0.447239395172066 0.327351109951482 no 1518 0.333722507940521 1082 NA FT CELL CYCLE MITOCHONDRION Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p G1/S transition of mitotic cell cycle protein serine/threonine phosphatase activity mitochondrion|cytoplasm YML099C YML099C ARG81 hom Verified 0.446841055118607 0.327494913054854 no 1519 0.321467727444208 1123 NA FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p arginine metabolic process|negative regulation of calcium ion-dependent exocytosis transcription cofactor activity nucleus YER069W YER069W ARG5,6 hom Verified 0.446063683135946 0.327775622621915 no 1520 0.319098175783445 1046 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine ornithine biosynthetic process|arginine biosynthetic process|regulation of transcription, DNA-dependent N-acetyl-gamma-glutamyl-phosphate reductase activity|acetylglutamate kinase activity mitochondrion|mitochondrial matrix YBR235W YBR235W VHC1 hom Verified 0.443681661359114 0.328636377975636 no 1521 0.339287992760237 1096 NA Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family transmembrane transport ion transmembrane transporter activity integral to membrane YDL222C YDL222C FMP45 hom Verified 0.442923448082055 0.328910553248607 no 1522 0.306355166673453 1030 NA FT CELL WALL ORG/BIOGENESIS MITOCHONDRION|PLASMA MEMBRANE Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C fungal-type cell wall organization|ascospore formation molecular_function cell cortex|mitochondrion|integral to membrane|plasma membrane YDR480W YDR480W DIG2 hom Verified 0.442592763064899 0.329030160133471 no 1523 0.315653718438375 1070 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p negative regulation of transcription from RNA polymerase II promoter by pheromones|Unknown transcription factor binding nucleus YMR118C_p YMR118C SHH3 hom Uncharacterized 0.442474665589847 0.329072879559777 no 1524 0.315042464216628 1078 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase biological_process molecular_function integral to membrane YDL101C YDL101C DUN1 hom Verified 0.442407685509051 0.329097109271649 no 1525 0.337114817680759 1143 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair protein phosphorylation|replication fork protection|cell cycle checkpoint|DNA damage checkpoint protein kinase activity nucleus YFR012W-A_p YFR012W-A YFR012W-A hom Uncharacterized 0.441964511817703 0.329257443242925 no 1526 0.302494681993916 1061 NA Putative protein of unknown function; identified by homology biological_process molecular_function cellular_component YLR200W YLR200W YKE2 hom Verified 0.441654738724421 0.329369533415921 no 1527 0.36237015806887 1214 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS NUCLEUS Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin protein folding|tubulin complex assembly tubulin binding prefoldin complex|cytoplasm YNL130C-A_p YNL130C-A DGR1 hom Uncharacterized 0.441512044792618 0.329421171812711 no 1528 0.316377146387636 1066 NA FT MITOCHONDRION Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose biological_process molecular_function cellular_component YER135C_d YER135C YER135C hom Dubious 0.439837804388293 0.330027292445063 no 1529 0.332179965256971 1099 NA Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Unknown Unknown Unknown YBR284W_p YBR284W YBR284W hom Uncharacterized 0.439242245993983 0.330243008542898 no 1530 0.333789237958059 1101 NA FT NUCLEOTIDE METABOLISM Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication biological_process AMP deaminase activity|molecular_function cellular_component YBL070C_d YBL070C YBL070C hom Dubious 0.438528130029451 0.330501741560502 no 1531 0.337599791728458 1144 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YHL021C YHL021C AIM17 hom Verified 0.438426072550722 0.330538724863992 no 1532 0.323825125933688 1059 NA FT OXIDATION-REDUCTION PROCESS MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YGL013C YGL013C PDR1 hom Verified 0.437877739924566 0.330737456429098 no 1533 0.428172643627108 1410 NA FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS RSC COMPLEX Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Unknown|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YGR255C YGR255C COQ6 hom Verified 0.43657841271158 0.331208560532324 no 1534 0.33255439335766 1128 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM MITOCHONDRION Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) ubiquinone biosynthetic process N,N-dimethylaniline monooxygenase activity mitochondrion|mitochondrial inner membrane YNL034W_p YNL034W YNL034W hom Uncharacterized 0.433603466860054 0.332288208403283 no 1535 0.330100564423915 1095 NA Putative protein of unknown function; YNL034W is not an essential gene biological_process molecular_function cellular_component YNR058W YNR058W BIO3 hom Verified 0.432489882302049 0.332692702093086 no 1536 0.342656017995108 1166 NA FT KETONE METABOLISM|COFACTOR METABOLISM 7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis biotin biosynthetic process adenosylmethionine-8-amino-7-oxononanoate transaminase activity cytoplasm YCL001W-A_p YCL001W-A YCL001W-A hom Uncharacterized 0.432241677255838 0.332782885602215 no 1537 0.348241370438783 1197 NA Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III biological_process molecular_function cellular_component YKL157W YKL157W APE2 hom Verified 0.431992445609433 0.332873451855028 no 1538 0.340785882182133 1117 NA FT PROTEOLYSIS MITOCHONDRION Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication peptide catabolic process metalloaminopeptidase activity mitochondrion|extracellular region|cell wall-bounded periplasmic space YKL216W YKL216W URA1 hom Verified 0.431721143730043 0.332972049114393 no 1539 0.319924868194079 1080 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid 'de novo' pyrimidine nucleobase biosynthetic process dihydroorotate dehydrogenase activity extrinsic to membrane|cytoplasm YGR107W_d YGR107W YGR107W hom Dubious 0.431077194478064 0.333206121112653 no 1540 0.340607318118837 1163 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR248W_d YOR248W YOR248W hom Dubious 0.430616242199376 0.33337371463418 no 1541 0.32940088541492 1094 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR236C_d YLR236C YLR236C hom Dubious 0.430536747238242 0.333402620861363 no 1542 0.324291650737169 1078 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL047C_p YOL047C YOL047C hom Uncharacterized 0.430293203243462 0.333491185311955 no 1543 0.32751320041806 1067 NA Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function integral to membrane|cytoplasm YPL090C YPL090C RPS6A hom Verified 0.430108449658017 0.333558376899661 no 1544 0.336826655569796 1151 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome small-subunit processome|cytosolic small ribosomal subunit|90S preribosome YKR042W YKR042W UTH1 hom Verified 0.430018464398133 0.333591104869395 no 1545 0.323933493322294 1113 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL WALL ORG/BIOGENESIS|CELL DIVISION|GENE SILENCING MITOCHONDRION Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication mitochondrion degradation|fungal-type cell wall biogenesis|negative regulation of chromatin silencing involved in replicative cell aging molecular_function mitochondrial outer membrane|fungal-type cell wall YIL017C YIL017C VID28 hom Verified 0.428632555803515 0.334095324744582 no 1546 0.328197992327248 1101 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS NUCLEUS GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|vacuolar protein catabolic process|ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function nucleus|GID complex|cytoplasm YLR304C YLR304C ACO1 hom Verified 0.42831485535962 0.334210952444207 no 1547 0.39295019982446 1296 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy propionate metabolic process|glutamate biosynthetic process|tricarboxylic acid cycle|mitochondrial genome maintenance|citrate metabolic process double-stranded DNA binding|aconitate hydratase activity|single-stranded DNA binding mitochondrial nucleoid|cytosol|mitochondrion|mitochondrial matrix YOR101W YOR101W RAS1 hom Verified 0.428073189382353 0.334298917788134 no 1548 0.309010587824724 1080 NA FT SIGNALING|NUCLEOTIDE METABOLISM PLASMA MEMBRANE GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress adenylate cyclase-activating G-protein coupled receptor signaling pathway|replicative cell aging|Ras protein signal transduction GTPase activity plasma membrane YCR017C YCR017C CWH43 hom Verified 0.427387711608368 0.334548478159934 no 1549 0.385691430431974 1278 NA FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion fungal-type cell wall organization|GPI anchor biosynthetic process molecular_function integral to membrane|integral to plasma membrane|cellular bud neck|cellular bud tip YGR159C YGR159C NSR1 hom Verified 0.427073709225295 0.334662820736378 no 1550 0.339526886651571 1121 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis ribosomal small subunit assembly|rRNA processing single-stranded DNA binding|RNA binding mitochondrion|nucleolus|nucleus YLL062C YLL062C MHT1 hom Verified 0.426427911463917 0.334898033320239 no 1551 0.333501116354962 1112 NA FT KETONE METABOLISM|AMINO ACID METABOLISM S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio sulfur amino acid metabolic process homocysteine S-methyltransferase activity cytoplasm YOL112W YOL112W MSB4 hom Verified 0.426195593410133 0.334982664094644 no 1552 0.336907794422307 1155 NA FT SIGNALING|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization actin filament organization Rab GTPase activator activity incipient cellular bud site|polarisome|cellular bud tip YEL041W YEL041W YEF1 hom Verified 0.42558953967283 0.335203481882633 no 1553 0.355813962276421 1156 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication NADP biosynthetic process NAD+ kinase activity|NADH kinase activity cellular_component YDR455C_d YDR455C YDR455C hom Dubious 0.424982580506 0.335424686657609 no 1554 0.368746880463001 1243 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Unknown Unknown Unknown YPR148C YPR148C YPR148C hom Verified 0.424729198082877 0.335517048146289 no 1555 0.319896970054574 1090 NA Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process molecular_function ribosome|cytoplasm YHR100C YHR100C GEP4 hom Verified 0.423665761836151 0.335904794098696 no 1556 0.383408655808191 1268 NA FF|FT LIPID METABOLISM|CARBOHYDRATE METABOLISM MITOCHONDRION Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT cardiolipin biosynthetic process|phosphorylated carbohydrate dephosphorylation phosphatidylglycerophosphatase activity mitochondrion|mitochondrial matrix YOR042W YOR042W CUE5 hom Verified 0.423576023431573 0.335937522163421 no 1557 0.338673538782807 1144 NA Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern biological_process ubiquitin binding cytoplasm YOR263C_d YOR263C YOR263C hom Dubious 0.423429613961147 0.335990921132251 no 1558 0.335677870124869 1156 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Unknown Unknown Unknown YJR139C YJR139C HOM6 hom Verified 0.422827792275192 0.336210454382629 no 1559 0.349613971406514 1192 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS NUCLEUS Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions threonine biosynthetic process|methionine biosynthetic process|homoserine biosynthetic process homoserine dehydrogenase activity nucleus|cytoplasm YIL155C YIL155C GUT2 hom Verified 0.42234334359197 0.336387212757528 no 1560 0.31524480026616 1042 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner NADH oxidation|replicative cell aging|glycerol metabolic process glycerol-3-phosphate dehydrogenase activity mitochondrion|integral to mitochondrial outer membrane YLL052C YLL052C AQY2 hom Verified 0.420670616035143 0.336997810354363 no 1561 0.337369602926055 1141 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains water transport water channel activity plasma membrane|endoplasmic reticulum membrane YDR351W YDR351W SBE2 hom Verified 0.42017613535521 0.337178393557611 no 1562 0.338623503532665 1147 NA FT PROTEIN LOCALIZATION|CELL WALL ORG/BIOGENESIS GOLGI APPARATUS Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth fungal-type cell wall organization molecular_function Golgi apparatus YLR327C YLR327C TMA10 hom Verified 0.419949179986832 0.33726128970018 no 1563 0.339470852016163 1133 NA FT NUCLEUS|MITOCHONDRION Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|nucleus|cytoplasm YGR167W YGR167W CLC1 hom Verified 0.418392030089524 0.337830256353026 no 1564 0.363011652070669 1219 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W endocytosis|vesicle-mediated transport structural molecule activity clathrin vesicle coat YKR077W YKR077W MSA2 hom Verified 0.417693087238452 0.33808576356607 no 1565 0.338054642265694 1143 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation regulation of transcription involved in G1 phase of mitotic cell cycle molecular_function nucleus|cytoplasm YER019W YER019W ISC1 hom Verified 0.417634354062505 0.338107237605133 no 1566 0.347009362568104 1151 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM|MITOCHONDRION Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance response to salt stress|ceramide biosynthetic process|sphingolipid catabolic process inositol phosphosphingolipid phospholipase activity mitochondrion|endoplasmic reticulum|integral to mitochondrial outer membrane YCL027W YCL027W FUS1 hom Verified 0.417304903161786 0.338227701291434 no 1567 0.311224309033828 1030 NA FT PROTEIN LOCALIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate regulation of termination of mating projection growth|cytogamy|cortical protein anchoring molecular_function integral to plasma membrane|mating projection tip YGL260W_p YGL260W YGL260W hom Uncharacterized 0.415895803163705 0.338743125337165 no 1568 0.329987552228337 1122 NA Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium biological_process molecular_function cellular_component YGR181W YGR181W TIM13 hom Verified 0.415808138243186 0.338775201618604 no 1569 0.311658262068003 1054 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity mitochondrion|integral to membrane|mitochondrial intermembrane space protein transporter complex|mitochondrial intermembrane space YBR148W YBR148W YSW1 hom Verified 0.41537108760757 0.338935134327342 no 1570 0.316422607845758 1065 NA FT MEMBRANE ORGANIZATION Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane ascospore-type prospore membrane assembly molecular_function prospore membrane YOR135C_d YOR135C IRC14 hom Dubious 0.415096641605182 0.339035578935408 no 1571 0.317473862417871 1058 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Unknown Unknown Unknown YCR025C_d YCR025C YCR025C hom Dubious 0.414051499248714 0.339418195740935 no 1572 0.359345809317142 1233 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Unknown Unknown Unknown YCR099C_p YCR099C YCR099C hom Uncharacterized 0.413909913543123 0.33947004168361 no 1573 0.377547131187487 1264 NA Putative protein of unknown function biological_process molecular_function integral to membrane YKL204W YKL204W EAP1 hom Verified 0.412926074690822 0.339830388277854 no 1574 0.361936478603995 1223 NA FT TRANSLATION eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade negative regulation of translation eukaryotic initiation factor 4E binding mRNA cap binding complex|cytoplasmic stress granule YBR084W YBR084W MIS1 hom Verified 0.412063101017164 0.340146586636393 no 1575 0.323755085112849 1095 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase nucleobase-containing compound metabolic process|folic acid biosynthetic process formate-tetrahydrofolate ligase activity|methylenetetrahydrofolate dehydrogenase (NADP+) activity|methenyltetrahydrofolate cyclohydrolase activity mitochondrion YIL130W YIL130W ASG1 hom Verified 0.411893697001423 0.340208670421955 no 1576 0.33675849088897 1130 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance biological_process sequence-specific DNA binding nucleus YBR255W YBR255W MTC4 hom Verified 0.411722650591932 0.34027136051315 no 1577 0.357317457568115 1183 NA Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 biological_process molecular_function cytoplasm YOL137W YOL137W BSC6 hom Verified 0.410612107942641 0.340678491987179 no 1578 0.356535147223696 1197 NA FT GOLGI APPARATUS Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function COPI-coated vesicle|integral to membrane|clathrin-coated vesicle YGR187C YGR187C HGH1 hom Verified 0.409297865843766 0.341160540756008 no 1579 0.345830511891376 1149 NA Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function cytoplasm YKL075C_p YKL075C YKL075C hom Uncharacterized 0.407916045736094 0.341667656062192 no 1580 0.349791029190181 1183 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin response to drug molecular_function cytoplasm YDL131W YDL131W LYS21 hom Verified 0.406903692215357 0.342039362035774 no 1581 0.317595853366168 1093 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS|MITOCHONDRION Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication lysine biosynthetic process via aminoadipic acid homocitrate synthase activity nucleus YDL076C YDL076C RXT3 hom Verified 0.406646562069644 0.342133796946588 no 1582 0.349861060947052 1166 NA FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase I promoter molecular_function Rpd3L-Expanded complex|nucleus|Rpd3L complex YAL031C YAL031C GIP4 hom Verified 0.405789960479882 0.342448467970465 no 1583 0.333559781658027 1103 NA FT CHROMOSOME SEGREGATION Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate chromosome segregation|regulation of phosphoprotein phosphatase activity protein phosphatase 1 binding|protein phosphatase type 1 regulator activity cytoplasm YKR080W YKR080W MTD1 hom Verified 0.405600188688804 0.342518195099615 no 1584 0.351651029458367 1166 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline folic acid-containing compound biosynthetic process|one-carbon metabolic process|purine nucleobase biosynthetic process methylenetetrahydrofolate dehydrogenase (NAD+) activity cytosol YMR171C YMR171C EAR1 hom Verified 0.40463215650948 0.342873958963592 no 1585 0.346320949942494 1162 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function endosome YHR061C YHR061C GIC1 hom Verified 0.404552836237572 0.342903116331934 no 1586 0.340676943724517 1130 NA FT CELL CYCLE|SIGNALING|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress regulation of exit from mitosis|establishment of cell polarity|axial cellular bud site selection|Rho protein signal transduction Rho GTPase binding|Rho GTPase activator activity incipient cellular bud site|actin cap|mating projection tip|cellular bud neck|cellular bud tip YJL127C YJL127C SPT10 hom Verified 0.404547418051517 0.342905108039108 no 1587 0.347073284852661 1142 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box DNA repair|negative regulation of transcription from RNA polymerase II promoter|chromatin silencing at rDNA|chromatin remodeling|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette histone acetyltransferase activity|sequence-specific DNA binding nucleus YDR159W YDR159W SAC3 hom Verified 0.404467266498744 0.342934571993281 no 1588 0.409137965103004 1358 NA FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) ribosomal small subunit biogenesis|actin filament-based process|mRNA export from nucleus|mRNA 3'-end processing|mitosis|protein export from nucleus|transcription-coupled nucleotide-excision repair|nuclear retention of pre-mRNA at the site of transcription|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery protein binding transcription export complex 2|nuclear pore YDR058C YDR058C TGL2 hom Verified 0.4043387417648 0.342981820069549 no 1589 0.349611406225431 1186 NA FT PROTEIN LOCALIZATION|LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli triglyceride catabolic process triglyceride lipase activity mitochondrion YKL133C_p YKL133C YKL133C hom Uncharacterized 0.404029086020225 0.3430956653485 no 1590 0.357451602704541 1183 NA Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) biological_process molecular_function cellular_component YHL020C YHL020C OPI1 hom Verified 0.40316986671968 0.343411632848209 no 1591 0.356790416132528 1213 NA FT TRANSCRIPTION FROM RNA POL II|LIPID METABOLISM|SIGNALING ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance endoplasmic reticulum unfolded protein response|negative regulation of transcription from RNA polymerase II promoter|phospholipid biosynthetic process|positive regulation of transcription from RNA polymerase II promoter transcription corepressor activity nuclear membrane|nucleoplasm|nuclear envelope|endoplasmic reticulum|nucleus YDR505C YDR505C PSP1 hom Verified 0.40305339580543 0.343454472047359 no 1592 0.335674665452474 1109 NA FT MITOCHONDRION Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication biological_process molecular_function mitochondrion|cytoplasm YNL100W YNL100W AIM37 hom Verified 0.400831426937421 0.34427211961928 no 1593 0.371272680321719 1244 NA FT MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture cristae formation molecular_function mitochondrion|mitochondrial crista junction|integral to mitochondrial inner membrane YHR008C YHR008C SOD2 hom Verified 0.400233022383023 0.34449244720401 no 1594 0.422538301700954 1377 NA FF|FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated reactive oxygen species metabolic process|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|age-dependent response to reactive oxygen species involved in chronological cell aging superoxide dismutase activity mitochondrion|mitochondrial matrix YOR161C-C_p YOR161C-C YOR161C-C hom Uncharacterized 0.398867524692391 0.344995409616502 no 1595 0.332407338824826 1123 NA Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YPR030W YPR030W CSR2 hom Verified 0.398323020445142 0.345196046776648 no 1596 0.353329102656171 1189 NA FT VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|MEMBRANE ORGANIZATION|CELL WALL ORG/BIOGENESIS NUCLEUS Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication fungal-type cell wall organization|regulation of transcription from RNA polymerase II promoter|ubiquitin-dependent endocytosis ubiquitin protein ligase binding nucleus YJL126W YJL126W NIT2 hom Verified 0.396650886427797 0.345812461120767 no 1597 0.35006603187506 1178 NA Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member biological_process hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds cellular_component YML063W YML063W RPS1B hom Verified 0.39378251431518 0.346870807550447 no 1598 0.363668385471939 1204 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YNL218W YNL218W MGS1 hom Verified 0.392672264447904 0.347280779158285 no 1599 0.315925941630381 1066 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress regulation of DNA repair|DNA replication, Okazaki fragment processing|DNA strand renaturation ubiquitin binding|single-stranded DNA-dependent ATPase activity|enzyme activator activity nucleus|cytoplasm YCR059C YCR059C YIH1 hom Verified 0.392631554418847 0.347295815171161 no 1600 0.332798921503729 1127 NA FT TRANSLATION|PROTEIN PHOSPHORYLATION NUCLEUS Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT negative regulation of protein phosphorylation protein kinase inhibitor activity|ribosome binding|actin monomer binding ribosome|nucleus|polysome|cytoplasm YPL273W YPL273W SAM4 hom Verified 0.391509824164621 0.34771021420181 no 1601 0.338174732185851 1168 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM NUCLEUS S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio homocysteine metabolic process|L-methionine biosynthetic process from S-adenosylmethionine|S-adenosylhomocysteine metabolic process homocysteine S-methyltransferase activity nucleus|cytoplasm YOL101C YOL101C IZH4 hom Verified 0.391259773433185 0.347802614874355 no 1602 0.363327579638384 1224 NA FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress cellular zinc ion homeostasis|cellular lipid metabolic process metal ion binding integral to membrane|membrane YLR221C YLR221C RSA3 hom Verified 0.390945618295019 0.347918716714524 no 1603 0.334396701151961 1100 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus ribosomal large subunit assembly molecular_function nucleolus|preribosome, large subunit precursor YIR034C YIR034C LYS1 hom Verified 0.390635778345017 0.348033237764914 no 1604 0.366723348252014 1226 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity lysine biosynthetic process|lysine biosynthetic process via aminoadipic acid saccharopine dehydrogenase (NAD+, L-lysine-forming) activity|mRNA binding cytoplasm YDR251W YDR251W PAM1 hom Verified 0.387369883656984 0.349241198068199 no 1605 0.35324529763303 1164 NA FT OXIDATION-REDUCTION PROCESS SITE OF POLARIZED GROWTH Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype pseudohyphal growth molecular_function cellular bud|cellular bud neck YDR340W_d YDR340W YDR340W hom Dubious 0.387364459893227 0.349243205436977 no 1606 0.333004839920839 1108 NA Putative protein of unknown function Unknown Unknown Unknown YKL043W YKL043W PHD1 hom Verified 0.387269536409784 0.349278337892695 no 1607 0.325026592596616 1062 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication pseudohyphal growth|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity nucleus YLR318W YLR318W EST2 hom Verified 0.386975976796238 0.349386996419223 no 1608 0.345622464030398 1146 NA FT NUCLEUS|CHROMOSOME Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia telomere maintenance via telomerase|replicative cell aging telomerase activity|telomeric template RNA reverse transcriptase activity|telomeric DNA binding telomerase catalytic core complex|nucleolus|nucleus|telomerase holoenzyme complex YAL023C YAL023C PMT2 hom Verified 0.386761981974283 0.349466212512793 no 1609 0.374412373088098 1232 NA FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication ER-associated misfolded protein catabolic process|protein O-linked glycosylation|Unknown|protein exit from endoplasmic reticulum|protein O-linked mannosylation dolichyl-phosphate-mannose-protein mannosyltransferase activity integral to membrane|dolichyl-phosphate-mannose-protein mannosyltransferase complex YPR073C YPR073C LTP1 hom Verified 0.384631758841656 0.35025513042745 no 1610 0.335355542275454 1123 NA FT NUCLEUS Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine protein dephosphorylation protein tyrosine phosphatase activity nucleus|cytoplasm YDR428C YDR428C BNA7 hom Verified 0.383942753040413 0.35051043897221 no 1611 0.338690499744433 1116 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine NAD biosynthetic process arylformamidase activity cellular_component YGL094C YGL094C PAN2 hom Verified 0.382970821754994 0.350870699330408 no 1612 0.361395252506634 1247 NA FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEOLYSIS Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes mRNA 3'-end processing|postreplication repair|mRNA processing poly(A)-specific ribonuclease activity PAN complex|cytoplasm YJR120W YJR120W YJR120W hom Verified 0.382609940457971 0.351004499352309 no 1613 0.349573167130091 1196 NA FT OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p cellular respiration|sterol transport|mitochondrion organization molecular_function cellular_component YKR053C YKR053C YSR3 hom Verified 0.378059154145161 0.352693325294608 no 1614 0.358785564261025 1220 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication phospholipid dephosphorylation|sphingolipid biosynthetic process sphingosine-1-phosphate phosphatase activity integral to membrane|endoplasmic reticulum YDR144C YDR144C MKC7 hom Verified 0.377728417737948 0.35281617740918 no 1615 0.366479760169984 1217 NA FT CELL WALL ORG/BIOGENESIS|PROTEOLYSIS PLASMA MEMBRANE GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p proteolysis|fungal-type cell wall organization aspartic-type endopeptidase activity fungal-type cell wall YGR223C YGR223C HSV2 hom Verified 0.376497370744329 0.353273584894205 no 1616 0.356790495895261 1167 NA FT MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization piecemeal microautophagy of nucleus phosphatidylinositol binding endosome|fungal-type vacuole|extrinsic to membrane|cytoplasm YFL015C_d YFL015C YFL015C hom Dubious 0.376235691717614 0.353370841630244 no 1617 0.348266601460185 1136 NA Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Unknown Unknown Unknown YLR092W YLR092W SUL2 hom Verified 0.375373952530851 0.353691186926674 no 1618 0.354263689224789 1168 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates sulfate transport sulfate transmembrane transporter activity integral to membrane|plasma membrane YFR023W YFR023W PES4 hom Verified 0.374212881659462 0.354122970462852 no 1619 0.361787488387219 1176 NA FT NUCLEUS Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication biological_process molecular_function cellular_component YPL089C YPL089C RLM1 hom Verified 0.373649780287961 0.354332446385104 no 1620 0.392486425035782 1302 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING NUCLEUS MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication signal transduction|positive regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|DNA binding, bending|sequence-specific DNA binding nucleus YPL099C YPL099C AIM43 hom Verified 0.373102903291838 0.3545359289687 no 1621 0.34702879934012 1168 NA FT MITOCHONDRION Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YLR364W YLR364W GRX8 hom Verified 0.372873546562615 0.354621280630372 no 1622 0.32441887898203 1084 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic biological_process glutathione-disulfide reductase activity cytoplasm YKL032C YKL032C IXR1 hom Verified 0.372055641618646 0.354925711073205 no 1623 0.3985276403049 1314 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b DNA repair|cellular response to hypoxia|negative regulation of transcription from RNA polymerase II promoter damaged DNA binding|sequence-specific DNA binding nuclear chromatin YJR052W YJR052W RAD7 hom Verified 0.371996081684524 0.354947883355146 no 1624 0.357172667492567 1171 NA FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex nucleotide-excision repair, DNA damage recognition|protein ubiquitination involved in ubiquitin-dependent protein catabolic process damaged DNA binding|ubiquitin-protein ligase activity|DNA-dependent ATPase activity nucleotide-excision repair factor 4 complex|Cul3-RING ubiquitin ligase complex YDR346C YDR346C SVF1 hom Verified 0.371742455984567 0.355042305690254 no 1625 0.377243026900904 1272 NA FT RESPONSE TO OXIDATIVE STRESS NUCLEUS Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance cellular response to oxidative stress molecular_function nucleus|cytoplasm YNL067W-B_p YNL067W-B YNL067W-B hom Uncharacterized 0.371717592700789 0.355051562523698 no 1626 0.367905048414091 1240 NA Putative protein of unknown function biological_process molecular_function cellular_component YML021C YML021C UNG1 hom Verified 0.371195186530738 0.355246079009848 no 1627 0.358990589330047 1214 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus DNA repair uracil DNA N-glycosylase activity mitochondrion|nucleus YER066W_p YER066W RRT13 hom Uncharacterized 0.36998845700472 0.355695545532128 no 1628 0.334381332442614 1156 NA Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription biological_process molecular_function cellular_component YGL136C YGL136C MRM2 hom Verified 0.369282895015335 0.35595843687865 no 1629 0.339382357825407 1138 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS MITOCHONDRION Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ rRNA methylation rRNA (uridine-2'-O-)-methyltransferase activity mitochondrion YOL025W YOL025W LAG2 hom Verified 0.368852093956113 0.356118986402756 no 1630 0.364707704030881 1196 NA FT MITOCHONDRION Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 replicative cell aging|negative regulation of ubiquitin-protein ligase activity molecular_function mitochondrion YDR257C YDR257C RKM4 hom Verified 0.368216197911422 0.35635601666982 no 1631 0.338341437417838 1107 NA FT NUCLEUS Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein peptidyl-lysine monomethylation protein-lysine N-methyltransferase activity nucleus YEL038W YEL038W UTR4 hom Verified 0.367584653100003 0.356591479952812 no 1632 0.324514219446522 1109 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus L-methionine salvage from methylthioadenosine acireductone synthase activity nucleus|cytoplasm YNL274C YNL274C GOR1 hom Verified 0.367436114848939 0.35664686845513 no 1633 0.344106816862858 1136 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS NUCLEUS|MITOCHONDRION Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress glyoxylate catabolic process|metabolic process oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor|glyoxylate reductase activity mitochondrion|nucleus|cytoplasm YDR444W_p YDR444W YDR444W hom Uncharacterized 0.366458140338923 0.357011621283412 no 1634 0.359354998240891 1203 NA FT LIPID METABOLISM Putative hydrolase acting on ester bonds biological_process molecular_function cytoplasm YDR015C_d YDR015C YDR015C hom Dubious 0.365819655664508 0.357249825957312 no 1635 0.348601651639266 1170 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Unknown Unknown Unknown YLR095C YLR095C IOC2 hom Verified 0.364077395123686 0.357900108113074 no 1636 0.356077849977091 1217 NA FT CHROMATIN ORGANIZATION NUCLEUS Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif chromatin remodeling protein binding|nucleosome binding|ATPase activity|DNA binding ISW1 complex YJR115W_p YJR115W YJR115W hom Uncharacterized 0.363919895397327 0.357958913747853 no 1637 0.340250595755018 1139 NA Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication biological_process molecular_function cellular_component YBR090C_p YBR090C YBR090C hom Uncharacterized 0.363807142201721 0.358001014451597 no 1638 0.347619102170501 1188 NA FT NUCLEUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus biological_process molecular_function nucleus|cytoplasm YDR256C YDR256C CTA1 hom Verified 0.362813557450137 0.358372081804511 no 1639 0.353390342757112 1158 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation response to reactive oxygen species|age-dependent response to reactive oxygen species|hydrogen peroxide catabolic process catalase activity peroxisomal matrix|mitochondrial matrix YDL045W-A YDL045W-A MRP10 hom Verified 0.362514395373871 0.358483834054932 no 1640 0.38034741617722 1291 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs mitochondrial translation|mitochondrial respiratory chain complex assembly structural constituent of ribosome mitochondrial small ribosomal subunit YBR188C YBR188C NTC20 hom Verified 0.3598397732773 0.359483478980534 no 1641 0.357532673965524 1161 NA FT RNA PROCESSING NUCLEUS Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YLR247C YLR247C IRC20 hom Verified 0.359679131220505 0.359543549949196 no 1642 0.339197568660459 1143 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci double-strand break repair via synthesis-dependent strand annealing helicase activity mitochondrion|nucleus YHR043C YHR043C DOG2 hom Verified 0.357676693891666 0.360292638184507 no 1643 0.349358815741905 1163 NA FT CARBOHYDRATE METABOLISM NUCLEUS 2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed glucose metabolic process 2-deoxyglucose-6-phosphatase activity nucleus|cytoplasm YHR202W_p YHR202W YHR202W hom Uncharacterized 0.357656276942679 0.360300278693307 no 1644 0.361216971600597 1219 NA FT NUCLEOTIDE METABOLISM Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization biological_process molecular_function cytosol|fungal-type vacuole YDR152W YDR152W GIR2 hom Verified 0.356869041845216 0.360594923348117 no 1645 0.366966864007074 1235 NA FT TRANSLATION Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein cytoplasmic translation molecular_function polysome|cytoplasm YGL019W YGL019W CKB1 hom Verified 0.356053230609628 0.360900350767625 no 1646 0.383014287354534 1268 NA FF|FT RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases regulation of transcription from RNA polymerase I promoter|protein phosphorylation|regulation of transcription from RNA polymerase III promoter|response to DNA damage stimulus protein kinase regulator activity|protein serine/threonine kinase inhibitor activity protein kinase CK2 complex|UTP-C complex YLR108C YLR108C YLR108C hom Verified 0.355427332617353 0.361134737694808 no 1647 0.375861441431158 1250 NA FT NUCLEUS Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication biological_process molecular_function nucleus YDR181C YDR181C SAS4 hom Verified 0.354593246841037 0.361447168035619 no 1648 0.399889187833775 1325 NA FT CHROMATIN ORGANIZATION|PROTEOLYSIS|GENE SILENCING NUCLEUS|CHROMOSOME HISTONE ACETYLTRANSFERASE Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p chromatin silencing at telomere acetyltransferase activity|histone acetyltransferase activity nuclear chromatin|SAS acetyltransferase complex|nuclear chromosome, telomeric region YLR389C YLR389C STE23 hom Verified 0.354585514995304 0.36145006464902 no 1649 0.349278470592283 1183 NA FT PROTEOLYSIS Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family peptide pheromone maturation metallopeptidase activity|metalloendopeptidase activity extrinsic to membrane YIL097W YIL097W FYV10 hom Verified 0.354119247339505 0.361624759106684 no 1650 0.368307437189043 1245 NA FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|PROTEOLYSIS NUCLEUS Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin negative regulation of gluconeogenesis|proteasomal ubiquitin-dependent protein catabolic process|anti-apoptosis ubiquitin-protein ligase activity nucleus|GID complex|cytoplasm YKL207W YKL207W EMC3 hom Verified 0.352763230199633 0.362132975919578 no 1651 0.346928584651268 1148 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 protein folding in endoplasmic reticulum molecular_function ER membrane protein complex|integral to membrane YER051W YER051W JHD1 hom Verified 0.352384407765617 0.362274996914198 no 1652 0.355764763074131 1168 NA FT CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS NUCLEUS JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe histone demethylation methylated histone residue binding|histone demethylase activity (H3-K36 specific) cellular_component YNR033W YNR033W ABZ1 hom Verified 0.351941525472947 0.362441058067153 no 1653 0.346331107028501 1136 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress para-aminobenzoic acid biosynthetic process 4-amino-4-deoxychorismate synthase activity cytoplasm YBL059W_p YBL059W YBL059W hom Uncharacterized 0.351941397081617 0.362441106211944 no 1654 0.375339126097115 1233 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication biological_process molecular_function mitochondrion YPL150W_p YPL150W YPL150W hom Uncharacterized 0.350438054535481 0.3630049856941 no 1655 0.344054048522905 1129 NA FT PROTEIN PHOSPHORYLATION Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study protein phosphorylation protein kinase activity cellular_component YEL013W YEL013W VAC8 hom Verified 0.349529416146962 0.363345945408844 no 1656 0.353450354246833 1196 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions piecemeal microautophagy of nucleus|vacuole inheritance|CVT vesicle assembly|vacuole fusion, non-autophagic|macroautophagy|nucleus-vacuole junction assembly protein anchor fungal-type vacuole membrane|nucleus-vacuole junction|Myo2p-Vac17p-Vac8p transport complex YOR107W YOR107W RGS2 hom Verified 0.349284405097734 0.363437902510175 no 1657 0.375591815132242 1290 NA FT SIGNALING NUCLEUS|PLASMA MEMBRANE Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p adenylate cyclase-modulating G-protein coupled receptor signaling pathway GTPase activator activity nucleus|cytoplasm YIL139C YIL139C REV7 hom Verified 0.349118876715922 0.363500032776101 no 1658 0.347773813371995 1138 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION|CHROMOSOME Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair error-prone translesion synthesis|error-free translesion synthesis DNA-directed DNA polymerase activity nuclear chromatin|mitochondrion|zeta DNA polymerase complex YNL212W YNL212W VID27 hom Verified 0.3478583341268 0.363973288927456 no 1659 0.362416637646913 1177 NA Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth biological_process molecular_function cytoplasm YBR298C YBR298C MAL31 hom Verified 0.347346677157275 0.364165443886592 no 1660 0.373256950299313 1244 NA FT CARBOHYDRATE METABOLISM Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C alpha-glucoside transport|transmembrane transport alpha-glucoside:hydrogen symporter activity integral to membrane|membrane YNL097C YNL097C PHO23 hom Verified 0.346649000700753 0.364427514285242 no 1661 0.346174200829027 1172 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 positive regulation of transcription from RNA polymerase II promoter in response to heat stress|negative regulation of chromatin silencing at silent mating-type cassette|negative regulation of transcription from RNA polymerase I promoter|negative regulation of chromatin silencing at telomere|negative regulation of chromatin silencing at rDNA|chromatin modification|positive regulation of transcription from RNA polymerase II promoter methylated histone residue binding histone deacetylase complex|Rpd3L-Expanded complex|nucleus|Rpd3L complex YIR044C YIR044C YIR044C hom pseudogene 0.344404657378016 0.365270993397073 no 1662 0.364417279676685 1215 NA Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family biological_process molecular_function cellular_component YLR246W YLR246W ERF2 hom Verified 0.344344783267778 0.365293504500834 no 1663 0.352742452172876 1159 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole protein targeting to membrane|protein palmitoylation palmitoyltransferase activity endoplasmic reticulum palmitoyltransferase complex|integral to endoplasmic reticulum membrane|integral to membrane YBR138C_p YBR138C YBR138C hom Uncharacterized 0.344169423596265 0.365359437833445 no 1664 0.319086021694341 1074 NA Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene biological_process molecular_function cytoplasm YLR214W YLR214W FRE1 hom Verified 0.343039912272987 0.365784217115166 no 1665 0.341951826421413 1151 NA FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY PLASMA MEMBRANE Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels iron ion transport|copper ion import ferric-chelate reductase activity integral to membrane|plasma membrane YML041C YML041C VPS71 hom Verified 0.342738202391273 0.365897710086644 no 1666 0.375330934631543 1261 NA FF|FT PROTEIN LOCALIZATION|CHROMATIN ORGANIZATION|VACUOLAR TRANSPORT NUCLEUS|CHROMOSOME Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting chromatin remodeling|protein targeting to vacuole|histone exchange nucleosome binding Swr1 complex|nucleus YPL225W YPL225W YPL225W hom Verified 0.340855215834057 0.366606290273271 no 1667 0.368393293363589 1215 NA Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress biological_process molecular_function ribosome|cytoplasm YGR112W YGR112W SHY1 hom Verified 0.34065292620707 0.366682440287355 no 1668 0.353402384071068 1192 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane YER121W_p YER121W YER121W hom Uncharacterized 0.340514755627115 0.366734456309963 no 1669 0.389079781252211 1235 NA Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy biological_process molecular_function cellular_component YIL168W YIL168W YIL168W hom pseudogene 0.338842279687442 0.367364274393923 no 1670 0.370112165575308 1216 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase serine family amino acid metabolic process L-serine ammonia-lyase activity cellular_component YMR251W-A YMR251W-A HOR7 hom Verified 0.338421806011798 0.3675226718636 no 1671 0.350346004693712 1163 NA FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication plasma membrane organization molecular_function endoplasmic reticulum|plasma membrane YLR396C YLR396C VPS33 hom Verified 0.337209199622801 0.367979601366332 no 1672 0.389161993222261 1307 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole regulation of SNARE complex assembly|endocytosis|regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vesicle fusion with vacuole|Golgi to vacuole transport|vacuole organization|vacuole fusion, non-autophagic|vesicle docking|protein targeting to vacuole phosphatidylinositol binding|ATP binding cytosol|fungal-type vacuole membrane|HOPS complex|fungal-type vacuole|CORVET complex YNL138W YNL138W SRV2 hom Verified 0.336930092362637 0.368084799929674 no 1673 0.440990948000592 1447 NA FT SIGNALING|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis cytoskeleton organization|Ras protein signal transduction cytoskeletal protein binding|adenylate cyclase binding mating projection tip|actin cortical patch YOL088C YOL088C MPD2 hom Verified 0.336104502933449 0.368396031454072 no 1674 0.427847760392936 1380 NA FT ENDOPLASMIC RETICULUM Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p protein folding protein disulfide isomerase activity|protein disulfide oxidoreductase activity endoplasmic reticulum YBR276C YBR276C PPS1 hom Verified 0.335475910087546 0.368633056938383 no 1675 0.326764144426388 1103 NA FT CELL CYCLE|SIGNALING|PROTEIN PHOSPHORYLATION NUCLEUS Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle inactivation of MAPK activity|protein dephosphorylation|regulation of S phase of mitotic cell cycle protein tyrosine/serine/threonine phosphatase activity cellular_component YHR114W YHR114W BZZ1 hom Verified 0.335290589527558 0.368702945890613 no 1676 0.346866971964027 1160 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins response to salt stress|actin filament organization|endocytosis molecular_function mating projection tip|cytoplasm|actin cortical patch YJR060W YJR060W CBF1 hom Verified 0.335137981174039 0.368760501523932 no 1677 0.3750612118006 1238 NA FF|FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|AMINO ACID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CHROMOSOME KINETOCHORE Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress regulation of transcription from RNA polymerase II promoter in response to methionine|negative regulation of transcription from RNA polymerase II promoter|methionine biosynthetic process|chromosome segregation|Unknown|chromatin remodeling|negative regulation of ceramide biosynthetic process by negative regulation of transcription from RNA Polymerase II promoter RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|RNA polymerase II transcription factor binding transcription factor activity|RNA polymerase II repressing transcription factor binding|centromeric DNA binding chromosome, centromeric region|mitochondrion|nucleus|kinetochore YML058W-A YML058W-A HUG1 hom Verified 0.334547858280072 0.368983091715211 no 1678 0.363772108315522 1202 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress cell cycle arrest|cell cycle checkpoint|response to DNA damage stimulus molecular_function cytoplasm YDR111C_p YDR111C ALT2 hom Uncharacterized 0.333891867972307 0.369230578208141 no 1679 0.364119311477472 1183 NA FT KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p biological_process transaminase activity nucleus|cytoplasm YBL029W_p YBL029W YBL029W hom Uncharacterized 0.331320946522388 0.370201035470818 no 1680 0.379697104471092 1258 NA FT NUCLEUS Non-essential protein of unknown function biological_process molecular_function nucleus|cytoplasm YER131W YER131W RPS26B hom Verified 0.331041135489466 0.3703067070015 no 1681 0.370431876207356 1228 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YPL163C YPL163C SVS1 hom Verified 0.331001109028968 0.370321823922658 no 1682 0.388956014618807 1256 NA Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication response to chemical stimulus molecular_function fungal-type cell wall|fungal-type vacuole YOR299W YOR299W BUD7 hom Verified 0.330514484831204 0.370505624849992 no 1683 0.368598989613162 1197 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM GOLGI APPARATUS Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Golgi to plasma membrane transport|fungal-type cell wall chitin biosynthetic process|cellular bud site selection molecular_function trans-Golgi network transport vesicle|clathrin-coated vesicle|exomer complex YDR463W YDR463W STP1 hom Verified 0.330178295067313 0.370632623044254 no 1684 0.455671280588317 1497 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS|PLASMA MEMBRANE Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication positive regulation of transcription, DNA-dependent|tRNA splicing, via endonucleolytic cleavage and ligation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription plasma membrane|nucleus|cytoplasm YKL118W_d YKL118W YKL118W hom Dubious 0.330177940733312 0.370632756904009 no 1685 0.454142671701707 1492 NA FF Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Unknown Unknown Unknown YHR182W YHR182W YHR182W hom Verified 0.329535863265685 0.370875345654805 no 1686 0.388925551380704 1298 NA FT SIGNALING Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cytoplasm YOR253W YOR253W NAT5 hom Verified 0.329509513559076 0.370885302158807 no 1687 0.37425789496157 1237 NA FT RIBOSOME Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatA complex|cytosolic ribosome YGL079W_p YGL079W KXD1 hom Uncharacterized 0.329063117434192 0.371053990596719 no 1688 0.364475538871992 1197 NA Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome biological_process molecular_function endosome YPR075C YPR075C OPY2 hom Verified 0.32885631545634 0.371132147307552 no 1689 0.361125606525955 1200 NA FT CELL CYCLE|SIGNALING PLASMA MEMBRANE Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress cell cycle arrest in response to pheromone|osmosensory signaling pathway molecular_function integral to membrane|plasma membrane|fungal-type vacuole|cytoplasm YCR079W YCR079W PTC6 hom Verified 0.328263729708815 0.371356132779779 no 1690 0.381933873089504 1279 NA FT RESPONSE TO NUTRIENT LEVELS MITOCHONDRION Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase mitochondrion degradation|positive regulation of catalytic activity|macroautophagy|protein dephosphorylation protein serine/threonine phosphatase activity mitochondrion|mitochondrial matrix|mitochondrial intermembrane space YPL224C YPL224C MMT2 hom Verified 0.325052661256537 0.37257060795341 no 1691 0.359541755109814 1192 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p cellular iron ion homeostasis molecular_function mitochondrion|integral to membrane YFL041W-A_p YFL041W-A YFL041W-A hom Uncharacterized 0.324396949930917 0.372818764303336 no 1692 0.368916469079412 1219 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YJR037W_d YJR037W YJR037W hom Dubious 0.323840822057117 0.3730292743092 no 1693 0.358025834954999 1178 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Unknown Unknown Unknown YOR138C YOR138C RUP1 hom Verified 0.322764687933371 0.373436728891165 no 1694 0.385359327201109 1271 NA FT NUCLEUS Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress protein deubiquitination protein binding nucleus|cytoplasm YNL296W_d YNL296W YNL296W hom Dubious 0.322387031755697 0.373579753694858 no 1695 0.415200560053546 1361 NA Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Unknown Unknown Unknown YGR261C YGR261C APL6 hom Verified 0.32149510430927 0.373917610854863 no 1696 0.397867118385295 1316 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools vesicle-mediated transport|Golgi to vacuole transport molecular_function AP-3 adaptor complex YBR199W YBR199W KTR4 hom Verified 0.318520016712431 0.375045256770536 no 1697 0.345361630952423 1164 NA FT CARBOHYDRATE METABOLISM GOLGI APPARATUS Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YDL037C YDL037C BSC1 hom Verified 0.317187747578544 0.375550573155646 no 1698 0.368242777786041 1236 NA Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression biological_process molecular_function cellular_component YKL169C_d YKL169C YKL169C hom Dubious 0.317169774568334 0.375557391600942 no 1699 0.386820404140439 1266 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Unknown Unknown Unknown YJR014W YJR014W TMA22 hom Verified 0.314134938655429 0.376709277226833 no 1700 0.329639678798355 1077 NA FT TRANSLATION RIBOSOME Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress biological_process RNA binding ribosome|cytoplasm YHR143W YHR143W DSE2 hom Verified 0.313967102317352 0.37677301241219 no 1701 0.441741789463838 1446 NA FF|FT CELL WALL ORG/BIOGENESIS|CELL DIVISION Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP cytokinesis, completion of separation|pseudohyphal growth glucosidase activity extracellular region|fungal-type cell wall YPR171W YPR171W BSP1 hom Verified 0.312769974198999 0.377227714396704 no 1702 0.380697889335445 1272 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton actin cortical patch localization protein binding, bridging mating projection tip|membrane|cellular bud neck|actin cortical patch|cellular bud tip YKR098C YKR098C UBP11 hom Verified 0.312708149351951 0.377251201792912 no 1703 0.378792037427358 1277 NA FT PROTEOLYSIS Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins biological_process ubiquitin-specific protease activity cellular_component YMR319C YMR319C FET4 hom Verified 0.312227365043622 0.377433868305459 no 1704 0.410254916260383 1338 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Low-affinity Fe(II) transporter of the plasma membrane intracellular copper ion transport|zinc ion transport|low-affinity iron ion transport|copper ion import copper ion transmembrane transporter activity|iron ion transmembrane transporter activity integral to membrane|plasma membrane|integral to plasma membrane YKL185W YKL185W ASH1 hom Verified 0.311501805625992 0.377709585216592 no 1705 0.355541375885989 1203 NA FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|core promoter proximal region sequence-specific DNA binding Rpd3L-Expanded complex|nucleus|cellular bud|Rpd3L complex YNL141W YNL141W AAH1 hom Verified 0.31103405302258 0.377887367047166 no 1706 0.382683306525314 1267 NA FT NUCLEOTIDE METABOLISM NUCLEUS Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome adenine catabolic process|hypoxanthine salvage|purine-containing compound salvage adenine deaminase activity nucleus|cytoplasm YDR161W_p YDR161W YDR161W hom Uncharacterized 0.310585540806462 0.378057860349447 no 1707 0.381949420503219 1283 NA FT PROTEOLYSIS NUCLEUS Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) ER-associated protein catabolic process molecular_function nucleus|cytoplasm YDR535C_d YDR535C YDR535C hom Dubious 0.31026126755202 0.378181141393035 no 1708 0.411889368077386 1361 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Unknown Unknown Unknown YLR320W YLR320W MMS22 hom Verified 0.309782450654433 0.378363198961298 no 1709 0.38053208572287 1283 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation replication fork processing|double-strand break repair|recombinational repair|meiotic sister chromatid segregation|response to DNA damage stimulus molecular_function Cul8-RING ubiquitin ligase complex|nucleus YKL053W_d YKL053W YKL053W hom Dubious 0.309214351028063 0.378579238972257 no 1710 0.359848339202422 1205 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Unknown Unknown Unknown YGR127W_p YGR127W YGR127W hom Uncharacterized 0.308288245278948 0.378931504834314 no 1711 0.369614748036259 1243 NA Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response biological_process molecular_function cellular_component YCL033C YCL033C MXR2 hom Verified 0.306949421949211 0.379440935174203 no 1712 0.375554385051195 1260 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan cellular response to oxidative stress peptide-methionine (R)-S-oxide reductase activity mitochondrion YJL053W YJL053W PEP8 hom Verified 0.306246680709051 0.379708416301112 no 1713 0.394332114536311 1321 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress protein retention in Golgi apparatus|retrograde transport, endosome to Golgi protein transporter activity endosome|retromer complex, inner shell|retromer complex YOL106W_d YOL106W YOL106W hom Dubious 0.306173400627078 0.379736311874354 no 1714 0.375639018458542 1218 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YCR044C YCR044C PER1 hom Verified 0.305808593735336 0.379875192445922 no 1715 0.462404846170607 1504 NA FF|FT LIPID METABOLISM|ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 GPI anchor biosynthetic process|cellular manganese ion homeostasis molecular_function fungal-type vacuole membrane|integral to membrane|endoplasmic reticulum YGR240C YGR240C PFK1 hom Verified 0.305543139784377 0.379976259477864 no 1716 0.397371252993168 1296 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes proton transport|glycolysis 6-phosphofructokinase activity mitochondrion|6-phosphofructokinase complex|cytoplasm YML074C YML074C FPR3 hom Verified 0.303620443261459 0.380708537469549 no 1717 0.361075363722031 1202 NA FT CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p meiotic recombination checkpoint peptidyl-prolyl cis-trans isomerase activity|macrolide binding nucleolus YER053C YER053C PIC2 hom Verified 0.302835662436932 0.381007552036723 no 1718 0.351457761935807 1151 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature phosphate ion transport|transmembrane transport inorganic phosphate transmembrane transporter activity mitochondrion|integral to membrane YHR080C YHR080C YHR080C hom Verified 0.302793323474946 0.381023685908634 no 1719 0.387826596880947 1293 NA FT MITOCHONDRION Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication biological_process molecular_function mitochondrion|ribosome YFR030W YFR030W MET10 hom Verified 0.302488728203026 0.381139762423678 no 1720 0.379161793631693 1286 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide sulfate assimilation sulfite reductase (NADPH) activity sulfite reductase complex (NADPH) YJL175W_d YJL175W YJL175W hom Dubious 0.301487880024593 0.381521245335968 no 1721 0.377857867050725 1236 NA Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Unknown Unknown Unknown YKL068W YKL068W NUP100 hom Verified 0.300060168638673 0.382065630432506 no 1722 0.369356016576779 1241 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p positive regulation of transcription, DNA-dependent|mRNA export from nucleus|NLS-bearing substrate import into nucleus|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|protein import into nucleus|protein targeting to nuclear inner membrane|mRNA export from nucleus in response to heat stress nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YCR092C YCR092C MSH3 hom Verified 0.300058439864454 0.382066289754197 no 1723 0.380862180151639 1275 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability mismatch repair|mitotic recombination|meiotic mismatch repair|removal of nonhomologous ends|DNA recombination DNA insertion or deletion binding|mismatched DNA binding|double-strand/single-strand DNA junction binding|loop DNA binding|Y-form DNA binding MutSbeta complex|nucleus|cytoplasm YGR011W_d YGR011W YGR011W hom Dubious 0.300055293623109 0.382067489671838 no 1724 0.37750591280669 1219 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL017C YKL017C HCS1 hom Verified 0.299625716050764 0.382231333159981 no 1725 0.330442299486826 1081 NA FT NUCLEUS|CHROMOSOME Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities lagging strand elongation ATP-dependent 5'-3' DNA helicase activity DNA helicase A complex|alpha DNA polymerase:primase complex YFL011W YFL011W HXT10 hom Verified 0.299225040791821 0.382384172140641 no 1726 0.407633645052022 1356 NA FT MITOCHONDRION|PLASMA MEMBRANE Putative hexose transporter, expressed at low levels and expression is repressed by glucose hexose transport|transmembrane transport galactose transmembrane transporter activity|mannose transmembrane transporter activity|fructose transmembrane transporter activity|glucose transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YMR247W-A_p YMR247W-A YMR247W-A hom Uncharacterized 0.299222851178799 0.382385007426562 no 1727 0.375519506674494 1225 NA Putative protein of unknown function biological_process molecular_function cellular_component YHR001W-A YHR001W-A QCR10 hom Verified 0.298992015142009 0.382473069020217 no 1728 0.360451567351543 1202 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|mitochondrial respiratory chain complex III YGR217W YGR217W CCH1 hom Verified 0.298397375436121 0.382699945988065 no 1729 0.390546247885711 1294 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together calcium ion transport voltage-gated calcium channel activity|calcium channel activity integral to membrane|plasma membrane YDL001W YDL001W RMD1 hom Verified 0.29771642765518 0.382959802113503 no 1730 0.378075569633783 1270 NA FT CELL CYCLE Cytoplasmic protein required for sporulation biological_process molecular_function cytoplasm YBR272C YBR272C HSM3 hom Verified 0.297382503028329 0.383087250174872 no 1731 0.377239358142884 1263 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE NUCLEUS Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation mismatch repair|proteasome regulatory particle assembly molecular_function cytosol|proteasome regulatory particle, base subcomplex|nucleus|cytoplasm YFR054C_d YFR054C YFR054C hom Dubious 0.296830030601505 0.383298138539159 no 1732 0.35754826548364 1202 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR386W YDR386W MUS81 hom Verified 0.295248987986209 0.383901841003424 no 1733 0.431984891778283 1421 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p DNA repair|DNA topological change|reciprocal meiotic recombination|resolution of meiotic recombination intermediates|response to DNA damage stimulus crossover junction endodeoxyribonuclease activity|endonuclease activity Holliday junction resolvase complex|nucleus YER091C-A_d YER091C-A YER091C-A hom Dubious 0.295131820066902 0.383946591426218 no 1734 0.39441711144551 1297 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YIL134C-A_p YIL134C-A YIL134C-A hom Uncharacterized 0.294661098723345 0.384126391546165 no 1735 0.380938730226533 1282 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YPL006W YPL006W NCR1 hom Verified 0.293237162007475 0.384670440370733 no 1736 0.368905839477305 1224 NA FT LIPID METABOLISM Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein sphingolipid metabolic process sphingolipid transporter activity fungal-type vacuole membrane|integral to membrane YHL041W_d YHL041W YHL041W hom Dubious 0.292900774787538 0.384798998309629 no 1737 0.355527943559678 1209 NA Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Unknown Unknown Unknown YDR266C YDR266C HEL2 hom Verified 0.292573869195906 0.384923944767873 no 1738 0.39201997356643 1286 NA RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor biological_process molecular_function ribosome|cytoplasm YIL094C YIL094C LYS12 hom Verified 0.29253918370008 0.384937202599155 no 1739 0.387842702981631 1292 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate lysine biosynthetic process homoisocitrate dehydrogenase activity mitochondrion YKL188C YKL188C PXA2 hom Verified 0.291227589284901 0.385438631704658 no 1740 0.377387135752503 1240 NA FT NUCLEOTIDE METABOLISM Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder fatty acid transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|integral to peroxisomal membrane YOL129W YOL129W VPS68 hom Verified 0.289041100070141 0.386274962943712 no 1741 0.392079942922516 1300 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT MITOCHONDRION Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria late endosome to vacuole transport via multivesicular body sorting pathway|protein targeting to vacuole molecular_function Vps55/Vps68 complex|mitochondrion|fungal-type vacuole membrane|integral to membrane YFR053C YFR053C HXK1 hom Verified 0.288274426559025 0.386568340636545 no 1742 0.405619601729294 1334 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication mannose metabolic process|glucose import|glycolysis|fructose import|glucose metabolic process|fructose metabolic process hexokinase activity cytosol|mitochondrion|cytoplasm YGR077C YGR077C PEX8 hom Verified 0.287806498181976 0.386747431455952 no 1743 0.337183856564539 1149 NA FT PROTEIN LOCALIZATION|PEROXISOME ORGANIZATION Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p protein targeting to peroxisome|peroxisome organization peroxisome targeting sequence binding peroxisomal matrix|peroxisomal membrane|extrinsic to intraperoxisomal membrane YAL068C YAL068C PAU8 hom Verified 0.287310932060687 0.386937126402711 no 1744 0.375633290484988 1266 NA FT TRANSCRIPTION FROM RNA POL II Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YBL001C YBL001C ECM15 hom Verified 0.286725711381911 0.387161174489051 no 1745 0.380532594313655 1251 NA FT CELL WALL ORG/BIOGENESIS NUCLEUS Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis fungal-type cell wall organization molecular_function nucleus|cytoplasm YJR011C_p YJR011C YJR011C hom Uncharacterized 0.286648759482717 0.387190637842111 no 1746 0.382234938122991 1255 NA Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS biological_process molecular_function cellular_component YDL094C_d YDL094C YDL094C hom Dubious 0.286569254266042 0.387221079492315 no 1747 0.365891894808732 1228 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Unknown Unknown Unknown YGL208W YGL208W SIP2 hom Verified 0.286365984505779 0.387298912342574 no 1748 0.373911443292974 1258 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|SIGNALING|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS PLASMA MEMBRANE One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane cellular response to glucose starvation|signal transduction|protein phosphorylation|replicative cell aging|invasive growth in response to glucose limitation|regulation of protein complex assembly AMP-activated protein kinase activity AMP-activated protein kinase complex|plasma membrane|cytoplasm YPL102C_d YPL102C YPL102C hom Dubious 0.284697190785814 0.387938071526503 no 1749 0.381268512455352 1260 NA Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Unknown Unknown Unknown YOR351C YOR351C MEK1 hom Verified 0.281854771048572 0.3890274357295 no 1750 0.388883709882638 1298 NA FT CELL CYCLE|PROTEIN PHOSPHORYLATION NUCLEUS Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids meiosis|meiotic recombination checkpoint|protein phosphorylation protein serine/threonine kinase activity|protein kinase activity nucleus YPL154C YPL154C PEP4 hom Verified 0.281626349897149 0.389115016693949 no 1751 0.39361146286792 1317 NA FT MEMBRANE ORGANIZATION|PROTEOLYSIS|RESPONSE TO NUTRIENT LEVELS MITOCHONDRION Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates microautophagy|proteolysis involved in cellular protein catabolic process|cellular response to starvation peptidase activity fungal-type vacuole lumen|mitochondrion|fungal-type vacuole YJR153W YJR153W PGU1 hom Verified 0.280838149965743 0.389417270605692 no 1752 0.36523149767405 1203 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins pseudohyphal growth|pectin catabolic process polygalacturonase activity extracellular region YPL158C YPL158C AIM44 hom Verified 0.280753255633976 0.389449829340418 no 1753 0.42842621145206 1411 NA FT SITE OF POLARIZED GROWTH Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress biological_process molecular_function cellular bud neck YJL207C YJL207C LAA1 hom Verified 0.280667618759713 0.389482673641809 no 1754 0.392659716661012 1286 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene protein localization|retrograde transport, endosome to Golgi molecular_function AP-1 adaptor complex|ribosome|clathrin-coated vesicle YGL125W YGL125W MET13 hom Verified 0.280565434081002 0.389521865553877 no 1755 0.400772124270348 1329 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway methionine biosynthetic process methylenetetrahydrofolate reductase (NADPH) activity mitochondrion YPR159C-A_p YPR159C-A YPR159C-A hom Uncharacterized 0.28033368978006 0.389610752927111 no 1756 0.381581063075709 1250 NA Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YOR192C YOR192C THI72 hom Verified 0.279338566632755 0.389992505924234 no 1757 0.411430536818219 1347 NA Transporter of thiamine or related compound; shares sequence similarity with Thi7p thiamine transport transporter activity integral to membrane|membrane YBR239C_p YBR239C ERT1 hom Uncharacterized 0.277928409169026 0.390533657671546 no 1758 0.397890338017005 1303 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING|CARBOHYDRATE METABOLISM NUCLEUS Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition biological_process sequence-specific DNA binding nucleus|cytoplasm YGL080W YGL080W FMP37 hom Verified 0.277545326348378 0.390680703424803 no 1759 0.391227063979162 1293 NA FT MITOCHONDRION Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia mitochondrial pyruvate transport pyruvate transmembrane transporter activity mitochondrion|integral to mitochondrial inner membrane YKR070W_p YKR070W YKR070W hom Uncharacterized 0.277218767407429 0.390806064919189 no 1760 0.379961977126297 1223 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YOR296W_p YOR296W YOR296W hom Uncharacterized 0.277155275322858 0.390830439977032 no 1761 0.371965919406084 1227 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene biological_process molecular_function cytoplasm YPL098C YPL098C MGR2 hom Verified 0.276969795070727 0.390901649606894 no 1762 0.418803297636148 1385 NA FT MITOCHONDRION Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) protein import into mitochondrial matrix molecular_function mitochondrial inner membrane presequence translocase complex YLR392C YLR392C ART10 hom Verified 0.276829590837512 0.390955479282932 no 1763 0.361846487273322 1203 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene biological_process molecular_function cytoplasm YOR187W YOR187W TUF1 hom Verified 0.276069583410103 0.391247311040527 no 1764 0.456120327152274 1496 NA FT TRANSLATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans mitochondrial translational elongation|mitochondrial translation GTPase activity|translation elongation factor activity mitochondrion|mitochondrial matrix YPR078C_p YPR078C YPR078C hom Uncharacterized 0.27192610356865 0.392839419753644 no 1765 0.389966780299269 1288 NA Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible biological_process molecular_function cellular_component YHR167W YHR167W THP2 hom Verified 0.269908776327262 0.393615217574789 no 1766 0.411678187701345 1370 NA FT TRANSCRIPTION FROM RNA POL II|RNA LOCALIZATION NUCLEUS Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance mRNA export from nucleus|transcription elongation from RNA polymerase II promoter|DNA recombination nucleic acid binding THO complex part of transcription export complex|nucleoplasmic THO complex YER183C YER183C FAU1 hom Verified 0.269698615032498 0.393696063060911 no 1767 0.397969512127848 1313 NA FT COFACTOR METABOLISM MITOCHONDRION 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis folic acid-containing compound biosynthetic process 5-formyltetrahydrofolate cyclo-ligase activity mitochondrion YGR118W YGR118W RPS23A hom Verified 0.269013675652238 0.393959579412264 no 1768 0.398064528417737 1336 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit YMR001C-A_p YMR001C-A YMR001C-A hom Uncharacterized 0.26863427522093 0.394105566837673 no 1769 0.388814808187055 1267 NA Putative protein of unknown function biological_process molecular_function cellular_component YBR219C_p YBR219C YBR219C hom Uncharacterized 0.268067293304405 0.394323760448701 no 1770 0.396475207020271 1290 NA Putative protein of unknown function; YBR219C is not an essential gene biological_process molecular_function integral to membrane YML095C YML095C RAD10 hom Verified 0.267995998454096 0.394351199441157 no 1771 0.390926976480386 1287 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein mitotic recombination|meiotic mismatch repair|DNA strand renaturation|nucleotide-excision repair, DNA incision, 5'-to lesion|removal of nonhomologous ends|double-strand break repair via single-strand annealing, removal of nonhomologous ends single-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleotide-excision repair factor 1 complex|nucleus YER057C YER057C HMF1 hom Verified 0.266127529430026 0.395070496918414 no 1772 0.379113727526939 1237 NA FT NUCLEUS Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro biological_process molecular_function cytosol|nucleus|cytoplasm YLR307W YLR307W CDA1 hom Verified 0.265618532709168 0.395266505569538 no 1773 0.379528360222158 1244 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall ascospore wall assembly chitin deacetylase activity chitosan layer of spore wall YDR326C YDR326C YSP2 hom Verified 0.263909951974624 0.395924653435739 no 1774 0.399768545338042 1346 NA FT MITOCHONDRION Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication apoptotic process molecular_function mitochondrion YNL271C YNL271C BNI1 hom Verified 0.263390129721763 0.396124948723406 no 1775 0.423409681890554 1399 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 barbed-end actin filament capping|actin nucleation|actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assembly|establishment of mitotic spindle orientation|formin-nucleated actin cable assembly profilin binding incipient cellular bud site|actin filament|polarisome|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YPL003W YPL003W ULA1 hom Verified 0.26318447869362 0.396204196713989 no 1776 0.398776108153246 1316 NA Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation protein neddylation NEDD8 activating enzyme activity cellular_component YGL033W YGL033W HOP2 hom Verified 0.262852137320354 0.396332274120655 no 1777 0.411536242435388 1369 NA FF|FT CELL CYCLE NUCLEUS|CHROMOSOME Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair synapsis|reciprocal meiotic recombination protein binding|double-stranded DNA binding condensed nuclear chromosome YPL132W YPL132W COX11 hom Verified 0.261492675551712 0.396856298574974 no 1778 0.400328703081565 1298 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION RIBOSOME Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p protein complex assembly|aerobic respiration copper ion binding mitochondrial envelope|mitochondrion|mitochondrial ribosome|mitochondrial inner membrane YJL208C YJL208C NUC1 hom Verified 0.261407826930244 0.396889010905739 no 1779 0.400725399382822 1309 NA FT NUCLEUS|MITOCHONDRION Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy DNA catabolic process|RNA catabolic process|apoptotic process|DNA recombination ribonuclease activity|endodeoxyribonuclease activity|exodeoxyribonuclease activity mitochondrion|mitochondrial inner membrane|nucleus YMR038C YMR038C CCS1 hom Verified 0.261406183889625 0.396889644366777 no 1780 0.442293262025736 1455 NA FF|FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS NUCLEUS|MITOCHONDRION Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress intracellular copper ion transport superoxide dismutase copper chaperone activity cytosol|mitochondrial inner membrane|nucleus YGR268C YGR268C HUA1 hom Verified 0.260555840235825 0.397217523964459 no 1781 0.405015050600708 1340 NA Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly biological_process molecular_function cytoplasm YML009W-B_d YML009W-B YML009W-B hom Dubious 0.260320342931548 0.397308341004301 no 1782 0.401887859188808 1337 NA Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Unknown Unknown Unknown YIL114C YIL114C POR2 hom Verified 0.258525658437659 0.398000624613149 no 1783 0.394910344221479 1299 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication DNA transport|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to membrane YNL166C YNL166C BNI5 hom Verified 0.257829001384557 0.398269440561325 no 1784 0.386454539063067 1277 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner septin ring assembly|cytokinesis molecular_function cellular bud neck septin ring|cellular bud neck YLR170C YLR170C APS1 hom Verified 0.257348749083719 0.398454781499129 no 1785 0.412754794177719 1381 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YHL014C YHL014C YLF2 hom Verified 0.256711101083873 0.39870090062408 no 1786 0.400626642478359 1350 NA FT MITOCHONDRION Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process GTP binding mitochondrion YCR043C_p YCR043C YCR043C hom Uncharacterized 0.256115381462496 0.398930872655791 no 1787 0.405678975321492 1339 NA FT GOLGI APPARATUS|SITE OF POLARIZED GROWTH Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene biological_process molecular_function Golgi apparatus|mating projection tip YOR308C YOR308C SNU66 hom Verified 0.255938979690831 0.398998977660018 no 1788 0.397525460721775 1310 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 maturation of 5S rRNA|mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YLR361C-A_p YLR361C-A YLR361C-A hom Uncharacterized 0.255026242102031 0.399351415467837 no 1789 0.40741928571939 1351 NA Putative protein of unknown function biological_process molecular_function cellular_component YIL028W_d YIL028W YIL028W hom Dubious 0.251659143694687 0.400652269675453 no 1790 0.395407571562564 1303 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YML118W YML118W NGL3 hom Verified 0.251032740054647 0.400894398281741 no 1791 0.376996840705744 1232 NA 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication polyadenylation-dependent RNA catabolic process poly(A)-specific ribonuclease activity|endonuclease activity cellular_component YAL024C YAL024C LTE1 hom Verified 0.249690181504183 0.401413475889021 no 1792 0.421740528726474 1393 NA FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|SIGNALING|CELL DIVISION Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures regulation of exit from mitosis|mitotic cell cycle spindle orientation checkpoint|vesicle-mediated transport guanyl-nucleotide exchange factor activity cellular bud YKL092C YKL092C BUD2 hom Verified 0.249134212136748 0.401628483070747 no 1793 0.400189417378513 1327 NA FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM PLASMA MEMBRANE GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint bipolar cellular bud site selection|small GTPase mediated signal transduction|filamentous growth|cellular bud site selection|axial cellular bud site selection GTPase activator activity|Ras GTPase activator activity intracellular YKR014C YKR014C YPT52 hom Verified 0.249012599933418 0.401675517506369 no 1794 0.402298470994624 1340 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress endocytosis|protein targeting to vacuole GTPase activity late endosome YDR426C_d YDR426C YDR426C hom Dubious 0.247770967078725 0.402155809758929 no 1795 0.389858723889687 1276 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Unknown Unknown Unknown YDR216W YDR216W ADR1 hom Verified 0.247417345366381 0.402292625868924 no 1796 0.417913938280271 1362 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|LIPID METABOLISM|CHROMATIN ORGANIZATION|OXIDATION-REDUCTION PROCESS|RESPONSE TO NUTRIENT LEVELS|GENE SILENCING|PEROXISOME ORGANIZATION NUCLEUS Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization negative regulation of chromatin silencing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|chromatin organization|regulation of transcription by chromatin organization|positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter|positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter by oleic acid|cellular response to oleic acid|peroxisome organization|positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|TFIIB-class transcription factor binding|sequence-specific DNA binding|TFIID-class transcription factor binding|RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription nucleus YKL121W_p YKL121W DGR2 hom Uncharacterized 0.246079572299969 0.402810317943839 no 1797 0.401916619739762 1320 NA Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds biological_process molecular_function cellular_component YDR147W YDR147W EKI1 hom Verified 0.244536526074397 0.403407658361201 no 1798 0.417353764531411 1397 NA FT LIPID METABOLISM Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication phosphatidylethanolamine biosynthetic process|phosphatidylcholine biosynthetic process ethanolamine kinase activity|choline kinase activity cytoplasm YEL006W YEL006W YEA6 hom Verified 0.244341141998074 0.403483311091976 no 1799 0.406819685424551 1357 NA FT MITOCHONDRION Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog transport|NAD transmembrane transport transporter activity|NAD transporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YER087C-A_d YER087C-A YER087C-A hom Dubious 0.244027539933155 0.403604745387603 no 1800 0.395291103144901 1328 NA Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Unknown Unknown Unknown YDR368W YDR368W YPR1 hom Verified 0.243598180013538 0.403771018991017 no 1801 0.4046235071003 1359 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication cellular response to oxidative stress|D-xylose catabolic process|arabinose catabolic process glucose 1-dehydrogenase (NADP+) activity|alditol:NADP+ 1-oxidoreductase activity|carbonyl reductase (NADPH) activity nucleus|cytoplasm YML112W YML112W CTK3 hom Verified 0.243337138929151 0.403872118070391 no 1802 0.432027488294998 1419 NA FF|FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION NUCLEUS Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress mRNA 3'-end processing|positive regulation of translational fidelity|positive regulation of transcription from RNA polymerase I promoter|positive regulation of DNA-dependent transcription, elongation|protein phosphorylation cyclin-dependent protein kinase activity nucleoplasm|nucleolus|carboxy-terminal domain protein kinase complex YFL040W_p YFL040W YFL040W hom Uncharacterized 0.243158087080193 0.403941467102345 no 1803 0.405332290860878 1326 NA Putative transporter, member of the sugar porter family; YFL040W is not an essential gene transmembrane transport substrate-specific transmembrane transporter activity integral to membrane|membrane YCR060W YCR060W TAH1 hom Verified 0.24255561918995 0.404174832649655 no 1804 0.407177018197731 1321 NA FT PROTEIN COMPLEX ORG/BIOGENESIS NUCLEUS Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II protein folding|box C/D snoRNP assembly chaperone binding R2TP complex|nucleus|cytoplasm YOL159C-A YOL159C-A YOL159C-A hom Verified 0.242108521632172 0.404348037649581 no 1805 0.387946250723705 1254 NA FT NUCLEUS Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function nucleus|cytoplasm YGL226C-A YGL226C-A OST5 hom Verified 0.241992657848028 0.404392926186009 no 1806 0.404531169907627 1349 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YKR061W YKR061W KTR2 hom Verified 0.241446519140289 0.404604530960254 no 1807 0.39748857253523 1308 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication cell wall mannoprotein biosynthetic process|protein N-linked glycosylation mannosyltransferase activity Golgi apparatus YOR045W YOR045W TOM6 hom Verified 0.24088171840966 0.404823395799735 no 1808 0.406291020953149 1353 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial matrix protein channel activity mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YOR228C YOR228C YOR228C hom Verified 0.240392043738166 0.405013172788225 no 1809 0.413984178359551 1361 NA FT MITOCHONDRION Protein of unknown function, localized to the mitochondrial outer membrane biological_process molecular_function mitochondrial outer membrane|mitochondrion|integral to membrane YAL007C YAL007C ERP2 hom Verified 0.239335589836413 0.405422685236187 no 1810 0.410450430513257 1387 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles ER to Golgi vesicle-mediated transport molecular_function ER to Golgi transport vesicle YBR182C-A_p YBR182C-A YBR182C-A hom Uncharacterized 0.2393217738589 0.405428041400315 no 1811 0.4127002224013 1385 NA Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching biological_process molecular_function cellular_component YDR063W YDR063W AIM7 hom Verified 0.238795844521658 0.405631946311324 no 1812 0.403602573259508 1339 NA FT CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss actin filament debranching|negative regulation of Arp2/3 complex-mediated actin nucleation molecular_function nucleus|actin cortical patch|cytoplasm YKL135C YKL135C APL2 hom Verified 0.238648706202832 0.405688997013958 no 1813 0.420086247376704 1398 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Golgi to vacuole transport|vesicle-mediated transport clathrin binding AP-1 adaptor complex YHR206W YHR206W SKN7 hom Verified 0.237668894074414 0.406068955678625 no 1814 0.42169936122239 1399 NA FF|FT TRANSCRIPTION FROM RNA POL II|SIGNALING|RESPONSE TO OXIDATIVE STRESS NUCLEUS Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication response to singlet oxygen|regulation of transcription from RNA polymerase II promoter in response to oxidative stress|transcription, DNA-dependent|response to osmotic stress|regulation of cell size sequence-specific DNA binding|two-component response regulator activity|sequence-specific DNA binding transcription factor activity nucleus YDR127W YDR127W ARO1 hom Verified 0.23730381164461 0.406210552642692 no 1815 0.457965149359821 1490 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids aromatic amino acid family biosynthetic process shikimate kinase activity|shikimate 3-dehydrogenase (NADP+) activity|3-phosphoshikimate 1-carboxyvinyltransferase activity|3-dehydroquinate dehydratase activity|3-dehydroquinate synthase activity cytoplasm YMR029C YMR029C FAR8 hom Verified 0.237027455464514 0.406317745352011 no 1816 0.391384273784676 1271 NA FT CELL CYCLE|SIGNALING ENDOPLASMIC RETICULUM Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p re-entry into mitotic cell cycle after pheromone arrest molecular_function cellular_component YBR005W YBR005W RCR1 hom Verified 0.236583938922637 0.406489790699367 no 1817 0.390725683767776 1286 NA FT VESICLE-MEDIATED TRANSPORT|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication fungal-type cell wall organization|vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane YDR042C_p YDR042C YDR042C hom Uncharacterized 0.235952794966098 0.40673465007221 no 1818 0.399501345159913 1311 NA Putative protein of unknown function; expression is increased in ssu72-ts69 mutant biological_process molecular_function cellular_component YBL057C YBL057C PTH2 hom Verified 0.235904825305565 0.406753261932058 no 1819 0.424694644559187 1396 NA FT PROTEOLYSIS MITOCHONDRION One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth negative regulation of proteasomal ubiquitin-dependent protein catabolic process aminoacyl-tRNA hydrolase activity mitochondrial outer membrane|mitochondrion|cytoplasm YKL034W YKL034W TUL1 hom Verified 0.235744611882627 0.406815425035818 no 1820 0.407412853873805 1362 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|protein ubiquitination ubiquitin-protein ligase activity integral to membrane|Golgi apparatus YLR278C_p YLR278C YLR278C hom Uncharacterized 0.233363786397786 0.40773946558617 no 1821 0.415084278035748 1367 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene biological_process sequence-specific DNA binding nucleus YDR079W YDR079W PET100 hom Verified 0.232339445924113 0.408137188565866 no 1822 0.405029171261242 1348 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme mitochondrial respiratory chain complex IV assembly|aerobic respiration unfolded protein binding mitochondrion|mitochondrial inner membrane|integral to mitochondrial inner membrane YKR102W YKR102W FLO10 hom Verified 0.230771655565082 0.408746101246396 no 1823 0.403515636701479 1341 NA Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth filamentous growth of a population of unicellular organisms|flocculation via cell wall protein-carbohydrate interaction|flocculation|invasive growth in response to glucose limitation mannose binding fungal-type cell wall YCR020C-A YCR020C-A MAK31 hom Verified 0.23073201250827 0.408761501039632 no 1824 0.39342429492935 1314 NA Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity NatC complex YHR035W_p YHR035W YHR035W hom Uncharacterized 0.23061099811967 0.408808511317089 no 1825 0.380813192736852 1302 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM Putative protein of unknown function; not an essential gene biological_process molecular_function cellular_component YGR034W YGR034W RPL26B hom Verified 0.230547704567184 0.408833099390766 no 1826 0.415937988515672 1380 NA FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDL032W_d YDL032W YDL032W hom Dubious 0.229961432320857 0.409060869570268 no 1827 0.443415544728061 1472 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Unknown Unknown Unknown YKL025C YKL025C PAN3 hom Verified 0.228527179594188 0.409618214412261 no 1828 0.435269096310031 1437 NA FT RNA PROCESSING|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes DNA repair|mRNA 3'-end processing|postreplication repair poly(A)-specific ribonuclease activity PAN complex|cytoplasm YNL076W YNL076W MKS1 hom Verified 0.228164967741192 0.409758997421983 no 1829 0.410676989182024 1360 NA FT TRANSCRIPTION FROM RNA POL II|SIGNALING NUCLEUS Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling regulation of nitrogen utilization|negative regulation of transcription from RNA polymerase II promoter|Unknown|mitochondria-nucleus signaling pathway molecular_function cytoplasm YBR106W YBR106W PHO88 hom Verified 0.227353063769773 0.41007460715477 no 1830 0.439876628384168 1452 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations phosphate ion transport molecular_function mitochondrion|membrane YGL180W YGL180W ATG1 hom Verified 0.227237412099993 0.410119568931844 no 1831 0.41058036255933 1325 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEIN PHOSPHORYLATION|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p piecemeal microautophagy of nucleus|mitochondrion degradation|protein phosphorylation|CVT pathway|autophagic vacuole assembly protein serine/threonine kinase activity|protein kinase activity cytosol|pre-autophagosomal structure|Atg1p signaling complex YJL101C YJL101C GSH1 hom Verified 0.22570231994906 0.410716476792926 no 1832 0.402838527062682 1311 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|RESPONSE TO OXIDATIVE STRESS Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress response to cadmium ion|response to hydrogen peroxide|glutathione biosynthetic process glutamate-cysteine ligase activity intracellular YHR199C YHR199C AIM46 hom Verified 0.225181775361533 0.410918933271923 no 1833 0.486627552695062 1614 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YGR021W_p YGR021W YGR021W hom Uncharacterized 0.224902348279846 0.411027621215036 no 1834 0.417178863494874 1407 NA FT MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process molecular_function mitochondrion YBL086C_p YBL086C YBL086C hom Uncharacterized 0.224576267878502 0.411154464400722 no 1835 0.401736726338075 1359 NA Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery biological_process molecular_function cellular_component YLL030C_d YLL030C RRT7 hom Dubious 0.224335711821043 0.411248045119692 no 1836 0.402601544250554 1314 NA Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR143W YLR143W DPH6 hom Verified 0.223947500443402 0.411399077115939 no 1837 0.424977207141986 1410 NA Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene biological_process molecular_function cytoplasm YNL257C YNL257C SIP3 hom Verified 0.223595978450571 0.411535846590897 no 1838 0.443975779667176 1458 NA FT TRANSCRIPTION FROM RNA POL II Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication positive regulation of transcription from RNA polymerase II promoter transcription cofactor activity integral to membrane YER180C-A YER180C-A SLO1 hom Verified 0.223366215297524 0.411625248190383 no 1839 0.42018414101448 1363 NA FT PROTEIN LOCALIZATION Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO intracellular protein transport small GTPase regulator activity cytoplasm YGL043W YGL043W DST1 hom Verified 0.222979556317697 0.411775708828296 no 1840 0.414572516376658 1354 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION NUCLEUS General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress transcription elongation from RNA polymerase I promoter|transcription initiation from RNA polymerase II promoter|mRNA cleavage|maintenance of transcriptional fidelity during DNA-dependent transcription elongation from RNA polymerase II promoter|tRNA transcription from RNA polymerase III promoter|regulation of mRNA 3'-end processing|positive regulation of transcription elongation from RNA polymerase II promoter|transcription from RNA polymerase III promoter|transcription antitermination|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter RNA polymerase II regulatory region DNA binding|RNA polymerase II core binding|core RNA polymerase II recruiting transcription factor activity nucleus YGR254W YGR254W ENO1 hom Verified 0.222343000733311 0.412023439999374 no 1841 0.400369641369146 1338 NA FT MEMBRANE ORGANIZATION|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress regulation of vacuole fusion, non-autophagic|gluconeogenesis|glycolysis phosphopyruvate hydratase activity mitochondrion|phosphopyruvate hydratase complex|plasma membrane|fungal-type vacuole|cytoplasm YER032W YER032W FIR1 hom Verified 0.220492127065717 0.412743951603957 no 1842 0.437908057087739 1469 NA FT RNA PROCESSING SITE OF POLARIZED GROWTH Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate mRNA polyadenylation|RNA polyadenylation molecular_function cellular bud neck YJR126C YJR126C VPS70 hom Verified 0.219526455505693 0.413119986956662 no 1843 0.405685199135643 1343 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS|VACUOLAR TRANSPORT Protein of unknown function involved in vacuolar protein sorting protein targeting to vacuole molecular_function cellular_component YEL001C YEL001C IRC22 hom Verified 0.218855370985415 0.413381356220501 no 1844 0.407260222914393 1334 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci biological_process molecular_function endoplasmic reticulum YOR369C YOR369C RPS12 hom Verified 0.218709306896121 0.41343824932248 no 1845 0.400508101222814 1308 NA FT TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic small ribosomal subunit YHL025W YHL025W SNF6 hom Verified 0.217341550883922 0.413971088995246 no 1846 0.434472682848906 1444 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM NUCLEUS RSC COMPLEX Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions sucrose catabolic process|nucleotide-excision repair|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex|nucleus YBL013W YBL013W FMT1 hom Verified 0.216443329988633 0.414321097093925 no 1847 0.445667224830196 1473 NA FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate translational initiation|methionyl-tRNA aminoacylation methionyl-tRNA formyltransferase activity mitochondrion YPL015C YPL015C HST2 hom Verified 0.216149757343289 0.414435507780484 no 1848 0.419969441275811 1367 NA FT GENE SILENCING NUCLEUS Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export negative regulation of chromatin silencing at telomere|chronological cell aging|chromatin silencing at rDNA|negative regulation of mitotic recombination NAD-dependent histone deacetylase activity cytoplasm YLR036C_p YLR036C YLR036C hom Uncharacterized 0.21552861072216 0.414677604020648 no 1849 0.404501323505296 1350 NA Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein biological_process molecular_function integral to membrane YAL022C YAL022C FUN26 hom Verified 0.214672755535877 0.415011232645486 no 1850 0.417473707038851 1352 NA Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis nucleoside transport|nicotinamide riboside transport|transmembrane transport nicotinamide riboside transporter activity|nucleoside transmembrane transporter activity fungal-type vacuole membrane|integral to membrane|intracellular|membrane YHR117W YHR117W TOM71 hom Verified 0.213977839072315 0.415282169402067 no 1851 0.415022354769104 1374 NA FT MITOCHONDRION Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication biological_process protein transporter activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane YIR017C YIR017C MET28 hom Verified 0.213836216870671 0.415337390567853 no 1852 0.430570457670937 1451 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism regulation of transcription from RNA polymerase II promoter|regulation of sulfur amino acid metabolic process|Unknown RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YPR135W YPR135W CTF4 hom Verified 0.213584037684737 0.415435724125137 no 1853 0.487607515867382 1620 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion DNA repair|replicative cell aging|double-strand break repair via break-induced replication|DNA replication initiation|DNA-dependent DNA replication|mitotic sister chromatid cohesion chromatin binding|DNA binding nuclear chromosome|replication fork protection complex|nuclear replication fork|nucleus YML113W YML113W DAT1 hom Verified 0.21313869860984 0.41560939046656 no 1854 0.407599170622551 1361 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability negative regulation of transcription from RNA polymerase II promoter AT DNA binding nucleus YLR398C YLR398C SKI2 hom Verified 0.212035194127175 0.416039788784789 no 1855 0.41594255917727 1390 NA FT TRANSLATION Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear-transcribed mRNA catabolic process, non-stop decay RNA helicase activity Ski complex YNL025C YNL025C SSN8 hom Verified 0.211388616909521 0.416292019225089 no 1856 0.41936083309388 1355 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS NUCLEUS Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance positive regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription by galactose|positive regulation of transcription from RNA polymerase II promoter cyclin-dependent protein kinase regulator activity mediator complex YKR001C YKR001C VPS1 hom Verified 0.21099962218652 0.416443783085162 no 1857 0.452306839851538 1473 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT|PEROXISOME ORGANIZATION MITOCHONDRION|CYTOSKELETON Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis vacuolar transport|peroxisome fission|endocytosis|actin cytoskeleton organization|protein retention in Golgi apparatus|peroxisome organization|lipid tube assembly|protein targeting to vacuole GTPase activity mitochondrial outer membrane|fungal-type vacuole membrane|peroxisome|actin cortical patch YMR234W YMR234W RNH1 hom Verified 0.210994737592352 0.416445688858288 no 1858 0.404010304431758 1337 NA FT CELL WALL ORG/BIOGENESIS NUCLEUS Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) fungal-type cell wall organization|RNA catabolic process ribonuclease H activity intracellular|nucleus YGL211W YGL211W NCS6 hom Verified 0.210411654305752 0.416673198716369 no 1859 0.414849538248358 1380 NA FT RNA PROCESSING MITOCHONDRION Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae tRNA wobble position uridine thiolation|protein urmylation|tRNA wobble uridine modification tRNA binding cytosol|mitochondrion YOL100W YOL100W PKH2 hom Verified 0.209744463128942 0.416933560380526 no 1860 0.404071649483154 1320 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|PROTEIN PHOSPHORYLATION NUCLEUS Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p regulation of nuclear-transcribed mRNA poly(A) tail shortening|protein phosphorylation|MAPK cascade involved in cell wall biogenesis|cytoplasmic mRNA processing body assembly protein kinase activity cell cortex|eisosome|nucleus YKL137W YKL137W CMC1 hom Verified 0.209624235979924 0.416980481156645 no 1861 0.418218246463212 1390 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif mitochondrial respiratory chain complex IV assembly copper ion binding extrinsic to mitochondrial inner membrane|mitochondrial intermembrane space YHR175W-A_p YHR175W-A YHR175W-A hom Uncharacterized 0.208231856109863 0.417523968068014 no 1862 0.409779532627111 1339 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YJR078W YJR078W BNA2 hom Verified 0.207151177038379 0.417945897648668 no 1863 0.411672516344028 1366 NA FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp de novo NAD biosynthetic process from tryptophan tryptophan 2,3-dioxygenase activity|indoleamine 2,3-dioxygenase activity cytoplasm YML088W YML088W UFO1 hom Verified 0.206775627860845 0.418092545473699 no 1864 0.4176432475549 1365 NA FT RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|cellular response to methylmercury|response to DNA damage stimulus ubiquitin-protein ligase activity|protein binding, bridging SCF ubiquitin ligase complex|nucleus|cytoplasm YJL062W YJL062W LAS21 hom Verified 0.206477784559158 0.418208858106985 no 1865 0.422445472846054 1398 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity integral to endoplasmic reticulum membrane|integral to membrane|integral to plasma membrane YPL133C YPL133C RDS2 hom Verified 0.206392424877411 0.418242193764452 no 1866 0.401433688921292 1321 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS NUCLEUS Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source|positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter|cellular response to xenobiotic stimulus|positive regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus|cytoplasm YIL159W YIL159W BNR1 hom Verified 0.206059215820248 0.41837232806654 no 1867 0.414776022877164 1382 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 barbed-end actin filament capping|actin filament bundle assembly|formin-nucleated actin cable assembly profilin binding cellular bud neck YNL180C YNL180C RHO5 hom Verified 0.205801621739673 0.418472937176546 no 1868 0.410394499816762 1315 NA FT SIGNALING NUCLEUS|PLASMA MEMBRANE Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Rho protein signal transduction GTPase activity endomembrane system|fungal-type vacuole membrane|plasma membrane|nucleus|cytoplasm YLR182W YLR182W SWI6 hom Verified 0.205249822411878 0.418688472645908 no 1869 0.45117459842743 1479 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|positive regulation of reciprocal meiotic recombination|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity SBF transcription complex|MBF transcription complex|nucleus|cytoplasm YBR250W YBR250W SPO23 hom Verified 0.205070018571268 0.418758710166617 no 1870 0.421767456202316 1382 NA FT CELL CYCLE Protein of unknown function; associates with meiosis-specific protein Spo1p meiosis molecular_function cellular_component YEL040W YEL040W UTR2 hom Verified 0.204894756624154 0.418827175961199 no 1871 0.411515282011668 1374 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM SITE OF POLARIZED GROWTH|CYTOSKELETON Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck cell wall chitin metabolic process|fungal-type cell wall organization transferase activity, transferring glycosyl groups cellular bud neck septin ring|fungal-type cell wall YJR128W_d YJR128W YJR128W hom Dubious 0.204887917549658 0.418829847684383 no 1872 0.412437303926957 1386 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Unknown Unknown Unknown YPR065W YPR065W ROX1 hom Verified 0.204501493022416 0.418980812692596 no 1873 0.420766107823647 1380 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS|CHROMOSOME Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance negative regulation of transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter in response to stress RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|DNA binding, bending|sequence-specific DNA binding|RNA polymerase II transcription factor recruiting transcription factor activity nuclear chromatin YDL238C YDL238C GUD1 hom Verified 0.202884923711438 0.419612489178879 no 1874 0.415752394980628 1359 NA Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures guanine metabolic process guanine deaminase activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides cytoplasm YDR055W YDR055W PST1 hom Verified 0.202880476621818 0.419614227171248 no 1875 0.411798269783917 1323 NA FT CELL WALL ORG/BIOGENESIS PLASMA MEMBRANE Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 fungal-type cell wall organization molecular_function fungal-type cell wall|plasma membrane YNL091W YNL091W NST1 hom Verified 0.202603800544719 0.419722359603612 no 1876 0.417827260265648 1378 NA Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 response to salt stress molecular_function cytoplasm YLR053C_p YLR053C YLR053C hom Uncharacterized 0.201395261138678 0.420194760182717 no 1877 0.407449990235093 1328 NA Putative protein of unknown function biological_process molecular_function cellular_component YNL209W YNL209W SSB2 hom Verified 0.201114570486085 0.420304494579966 no 1878 0.379354481587305 1287 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RESPONSE TO NUTRIENT LEVELS Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication cellular response to glucose starvation|cytoplasmic translation|ribosomal subunit export from nucleus|rRNA processing|regulation of translational fidelity|'de novo' cotranslational protein folding unfolded protein binding|ATPase activity plasma membrane|polysome|cytoplasm YPR059C_d YPR059C YPR059C hom Dubious 0.200951802405288 0.420368130663788 no 1879 0.440320222534487 1451 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Unknown Unknown Unknown YNR048W YNR048W YNR048W hom Verified 0.20063533872879 0.420491861793494 no 1880 0.420636595165237 1402 NA Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication response to drug phospholipid-translocating ATPase activity integral to membrane YMR294W-A_d YMR294W-A YMR294W-A hom Dubious 0.200131567306336 0.420688842804024 no 1881 0.443892420586423 1458 NA Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Unknown Unknown Unknown YMR148W YMR148W OSW5 hom Verified 0.199195005928075 0.421055102918946 no 1882 0.399323447953651 1307 NA Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion biological_process molecular_function integral to membrane YOR140W YOR140W SFL1 hom Verified 0.198871612929607 0.421181587780643 no 1883 0.414774150883981 1371 NA FT TRANSCRIPTION FROM RNA POL II|GENE SILENCING NUCLEUS Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p gene silencing|Unknown|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus YGL107C YGL107C RMD9 hom Verified 0.198682676016462 0.4212554881965 no 1884 0.427583853447183 1403 NA FT TRANSLATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes translational initiation|regulation of mRNA stability|aerobic respiration molecular_function extrinsic to mitochondrial inner membrane|mitochondrion YHR030C YHR030C SLT2 hom Verified 0.198005099543152 0.421520536933326 no 1885 0.455093760145741 1511 NA FF|FT PROTEIN LOCALIZATION|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION NUCLEUS|SITE OF POLARIZED GROWTH Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway protein phosphorylation|endoplasmic reticulum unfolded protein response|signal transduction|UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|fungal-type cell wall organization|peroxisome degradation|regulation of transcription factor import into nucleus|barrier septum assembly|response to acid|regulation of cell size MAP kinase activity|protein serine/threonine kinase activity|protein kinase activity nucleus|cytoplasm|cellular bud tip YPL256C YPL256C CLN2 hom Verified 0.197338278815018 0.421781413047688 no 1886 0.414523614166687 1356 NA FT CELL CYCLE|SIGNALING|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) regulation of cyclin-dependent protein kinase activity|re-entry into mitotic cell cycle after pheromone arrest cyclin-dependent protein kinase regulator activity cyclin-dependent protein kinase holoenzyme complex|nucleus|cytoplasm YOR315W YOR315W SFG1 hom Verified 0.197158871555045 0.421851607296518 no 1887 0.420007007471494 1382 NA FT CELL CYCLE NUCLEUS Nuclear protein, putative transcription factor required for growth of superficial pseudohyphae (which do not invade the agar substrate) but not for invasive pseudohyphal growth; may act together with Phd1p; potential Cdc28p substrate mitotic cell cycle|pseudohyphal growth molecular_function nucleus|cytoplasm YDL240W YDL240W LRG1 hom Verified 0.196091439746316 0.42226929814372 no 1888 0.429399656324099 1420 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS MITOCHONDRION|SITE OF POLARIZED GROWTH Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis fungal-type cell wall biogenesis|small GTPase mediated signal transduction Rho GTPase activator activity mitochondrion|cellular bud neck|cytoplasm YLR207W YLR207W HRD3 hom Verified 0.195524604277798 0.422491138974403 no 1889 0.414845716995368 1360 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM UBIQUITIN LIGASE COMPLEX Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events ER-associated protein catabolic process ubiquitin-protein ligase activity Hrd1p ubiquitin ligase ERAD-M complex|luminal surveillance complex|Hrd1p ubiquitin ligase ERAD-L complex|endoplasmic reticulum membrane YDL022W YDL022W GPD1 hom Verified 0.194806239993807 0.422772318536091 no 1890 0.414036034825744 1407 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity NADH oxidation|intracellular accumulation of glycerol glycerol-3-phosphate dehydrogenase [NAD+] activity cytosol|peroxisome|nucleus YDR359C YDR359C EAF1 hom Verified 0.193554175236033 0.423262491198455 no 1891 0.45864134540285 1514 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS HISTONE ACETYLTRANSFERASE Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 DNA repair|cellular protein complex assembly|chromatin modification molecular_function histone acetyltransferase complex|NuA4 histone acetyltransferase complex YDL024C YDL024C DIA3 hom Verified 0.193503425297294 0.423282361876484 no 1892 0.436051897401253 1438 NA Protein of unknown function, involved in invasive and pseudohyphal growth pseudohyphal growth|invasive growth in response to glucose limitation acid phosphatase activity fungal-type cell wall YJL181W_p YJL181W YJL181W hom Uncharacterized 0.193461564060771 0.423298752410678 no 1893 0.392136743885075 1272 NA Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication biological_process molecular_function cellular_component YJL193W_p YJL193W YJL193W hom Uncharacterized 0.193434017819941 0.423309538060389 no 1894 0.426258030614154 1391 NA Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect biological_process molecular_function integral to membrane YPR047W YPR047W MSF1 hom Verified 0.193062904906011 0.423454851831603 no 1895 0.45203458113212 1517 NA FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase phenylalanyl-tRNA aminoacylation|mitochondrial phenylalanyl-tRNA aminoacylation phenylalanine-tRNA ligase activity mitochondrion YPL022W YPL022W RAD1 hom Verified 0.192556963960017 0.42365297592646 no 1896 0.425708821140594 1419 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein mitotic recombination|meiotic mismatch repair|nucleotide-excision repair, DNA incision, 5'-to lesion|double-strand break repair via single-strand annealing, removal of nonhomologous ends|removal of nonhomologous ends single-stranded DNA binding|single-stranded DNA specific endodeoxyribonuclease activity nucleotide-excision repair factor 1 complex|nucleus YDR101C YDR101C ARX1 hom Verified 0.192525561083733 0.423665273781841 no 1897 0.433283175631799 1424 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|PROTEOLYSIS NUCLEUS RIBOSOME Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex ribosomal large subunit biogenesis molecular_function nucleoplasm|cytosolic large ribosomal subunit|cytoplasm YJL051W YJL051W IRC8 hom Verified 0.192412941706477 0.423709377895137 no 1898 0.418879743458158 1369 NA Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc mitotic recombination molecular_function integral to membrane|cellular bud YDR247W YDR247W VHS1 hom Verified 0.191793196136476 0.423952100362177 no 1899 0.428933704009824 1396 NA FT CELL CYCLE|PROTEIN PHOSPHORYLATION Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p protein phosphorylation|G1/S transition of mitotic cell cycle protein serine/threonine kinase activity|protein kinase activity cytoplasm YOL164W-A_p YOL164W-A YOL164W-A hom Uncharacterized 0.191697129132211 0.423989727452257 no 1900 0.418318406320809 1365 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YPR126C_d YPR126C YPR126C hom Dubious 0.189806626122485 0.424730331696473 no 1901 0.425304853097892 1387 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YGL032C YGL032C AGA2 hom Verified 0.187748495731096 0.42553690604949 no 1902 0.426176232564062 1408 NA Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds agglutination involved in conjugation with cellular fusion cell adhesion molecule binding fungal-type cell wall YBR260C YBR260C RGD1 hom Verified 0.186771744915865 0.425919800674452 no 1903 0.420537026062665 1369 NA FT SIGNALING SITE OF POLARIZED GROWTH|CYTOSKELETON GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization response to acid|osmosensory signaling pathway Rho GTPase activator activity|phosphatidylinositol-4,5-bisphosphate binding|phosphatidylinositol-3,5-bisphosphate binding|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-4-phosphate binding|phosphatidylinositol-5-phosphate binding cellular bud|mating projection tip|actin cortical patch YPL164C YPL164C MLH3 hom Verified 0.186201328022812 0.42614344128012 no 1904 0.428062620433934 1403 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability mismatch repair|reciprocal meiotic recombination molecular_function nucleus|MutLbeta complex YOR357C YOR357C SNX3 hom Verified 0.186082298846866 0.426190111474173 no 1905 0.42550137894704 1392 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p protein localization|late endosome to Golgi transport phosphatidylinositol-3-phosphate binding cytosol|endosome YHL023C YHL023C NPR3 hom Verified 0.186043919118869 0.426205160016482 no 1906 0.444193133088909 1490 NA FT CELL CYCLE|SIGNALING|RESPONSE TO NUTRIENT LEVELS Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair meiosis|regulation of TOR signaling cascade|cellular response to amino acid starvation molecular_function Seh1-associated complex|extrinsic to fungal-type vacuolar membrane YER031C YER031C YPT31 hom Verified 0.185802700496933 0.426299743360504 no 1907 0.455412642860251 1496 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication early endosome to Golgi transport|vesicle-mediated transport|exocytosis GTPase activity mitochondrial outer membrane|endosome|Golgi apparatus YLR192C YLR192C HCR1 hom Verified 0.18470353199776 0.426730787856068 no 1908 0.455072930836798 1509 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) translation initiation factor activity eukaryotic translation initiation factor 3 complex YOR064C YOR064C YNG1 hom Verified 0.183811383122083 0.427080712927192 no 1909 0.429474256774698 1417 NA FT CHROMATIN ORGANIZATION NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 histone acetylation|chromatin modification histone acetyltransferase activity|methylated histone residue binding NuA3 histone acetyltransferase complex YLR046C_p YLR046C YLR046C hom Uncharacterized 0.183767715689214 0.42709784195828 no 1910 0.411429006635278 1363 NA Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance biological_process molecular_function integral to membrane YDL237W YDL237W AIM6 hom Verified 0.183624618428741 0.427153974396528 no 1911 0.430898573675818 1414 NA Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene biological_process molecular_function cellular_component YHR013C YHR013C ARD1 hom Verified 0.182968253433598 0.427411464088206 no 1912 0.429797290019024 1442 NA FT CHROMATIN ORGANIZATION RIBOSOME Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress histone acetylation|N-terminal protein amino acid acetylation peptide alpha-N-acetyltransferase activity cytosolic ribosome|NatA complex YIL095W YIL095W PRK1 hom Verified 0.182013973720441 0.427785879817489 no 1913 0.423710989051205 1408 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|actin cortical patch assembly|cytokinesis protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YHL015W-A_p YHL015W-A YHL015W-A hom Uncharacterized 0.181346052622017 0.428047980230383 no 1914 0.416620573531288 1372 NA Putative protein of unknown function biological_process molecular_function cellular_component YDR059C YDR059C UBC5 hom Verified 0.180496616250739 0.42838135530623 no 1915 0.431308419073005 1413 NA Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication protein polyubiquitination|response to stress ubiquitin-protein ligase activity proteasome complex YML102W YML102W CAC2 hom Verified 0.178876230782607 0.429017443420848 no 1916 0.429839816855326 1394 NA FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription DNA replication-dependent nucleosome assembly histone binding chromosome, centromeric region|CAF-1 complex YLR041W_d YLR041W YLR041W hom Dubious 0.178190480340746 0.429286692835615 no 1917 0.422301020634796 1407 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C Unknown Unknown Unknown YHR154W YHR154W RTT107 hom Verified 0.17715250711869 0.429694299686569 no 1918 0.443564355024418 1473 NA FT RESPONSE TO DNA DAMAGE NUCLEUS UBIQUITIN LIGASE COMPLEX Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress negative regulation of transposition, RNA-mediated|double-strand break repair molecular_function Cul8-RING ubiquitin ligase complex|nucleus YEL017C-A YEL017C-A PMP2 hom Verified 0.175388384686622 0.430387233402214 no 1919 0.436695580524476 1453 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication cation transport molecular_function membrane YKL221W YKL221W MCH2 hom Verified 0.174950945964639 0.430559089224916 no 1920 0.441037286073479 1472 NA Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport transport monocarboxylic acid transmembrane transporter activity|transporter activity integral to membrane|membrane YOR384W YOR384W FRE5 hom Verified 0.174667436202319 0.430670478260017 no 1921 0.439549013079034 1446 NA FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION|PLASMA MEMBRANE Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies biological_process ferric-chelate reductase activity mitochondrion|integral to membrane YMR194C-A_d YMR194C-A YMR194C-A hom Dubious 0.173992986350258 0.430935487120631 no 1922 0.429540716691867 1421 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR030C YDR030C RAD28 hom Verified 0.173547231799622 0.431110652735836 no 1923 0.433233743675879 1425 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair DNA repair molecular_function nucleus YMR158C-A_p YMR158C-A YMR158C-A hom Uncharacterized 0.173498307562658 0.431129879039193 no 1924 0.409299298780498 1317 NA Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene biological_process molecular_function cellular_component YNL291C YNL291C MID1 hom Verified 0.173132940210323 0.43127346668212 no 1925 0.425563076307524 1379 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOPLASMIC RETICULUM|PLASMA MEMBRANE N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer calcium ion transport stretch-activated, cation-selective, calcium channel activity|calcium channel activity endoplasmic reticulum|plasma membrane YIR028W YIR028W DAL4 hom Verified 0.172499394917951 0.431522468508014 no 1926 0.432174040106143 1432 NA Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation allantoin catabolic process|allantoin transport allantoin uptake transmembrane transporter activity integral to membrane|membrane YNL044W YNL044W YIP3 hom Verified 0.17225099876315 0.431620102892373 no 1927 0.434303171436371 1435 NA FT VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p ER to Golgi vesicle-mediated transport molecular_function Golgi apparatus|endoplasmic reticulum|ER to Golgi transport vesicle YNR029C_p YNR029C YNR029C hom Uncharacterized 0.172015087741926 0.431712833748521 no 1928 0.414618902536496 1385 NA Putative protein of unknown function, deletion confers reduced fitness in saline biological_process molecular_function cytoplasm YPL166W YPL166W ATG29 hom Verified 0.171893745859141 0.431760531742616 no 1929 0.430358012173123 1431 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress mitochondrion degradation|piecemeal microautophagy of nucleus|macroautophagy molecular_function pre-autophagosomal structure|cytoplasm YDL210W YDL210W UGA4 hom Verified 0.171129285296207 0.432061054589838 no 1930 0.423075171510343 1379 NA Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane gamma-aminobutyric acid transport|putrescine transport|transmembrane transport putrescine transmembrane transporter activity|gamma-aminobutyric acid:hydrogen symporter activity fungal-type vacuole membrane|integral to membrane YNL155W YNL155W YNL155W hom Verified 0.169799277611286 0.432583997530971 no 1931 0.422767688093805 1435 NA FT NUCLEUS Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus|cytoplasm YBR169C YBR169C SSE2 hom Verified 0.169487873163002 0.432706455092765 no 1932 0.391377927676309 1256 NA Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication protein folding|protein refolding adenyl-nucleotide exchange factor activity cytoplasm YNL055C YNL055C POR1 hom Verified 0.169240920690236 0.432803571974069 no 1933 0.445518432238665 1487 NA FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication DNA transport|mitochondrion organization|apoptotic process|ion transport|cell redox homeostasis voltage-gated anion channel activity mitochondrial outer membrane|mitochondrion|integral to mitochondrial outer membrane YNL020C YNL020C ARK1 hom Verified 0.168823784851622 0.432967624623995 no 1934 0.442160177628416 1446 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication regulation of endocytosis|actin filament organization|protein phosphorylation|cytokinesis|actin cortical patch assembly protein serine/threonine kinase activity|protein kinase activity mating projection tip|actin cortical patch YBL065W_d YBL065W YBL065W hom Dubious 0.168631127950509 0.433043397309675 no 1935 0.448100157041724 1465 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Unknown Unknown Unknown YDR484W YDR484W VPS52 hom Verified 0.168630813280597 0.433043521072552 no 1936 0.458382302587376 1511 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT GOLGI APPARATUS|CYTOSKELETON Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin actin filament-based process|Golgi to vacuole transport|retrograde transport, endosome to Golgi protein binding Golgi apparatus|GARP complex YIR009W YIR009W MSL1 hom Verified 0.168256081306455 0.433190911627269 no 1937 0.444945816041863 1475 NA FT RNA PROCESSING NUCLEUS U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members mRNA splicing, via spliceosome U2 snRNA binding U2 snRNP|U2-type prespliceosome YLR032W YLR032W RAD5 hom Verified 0.167912289402474 0.433326140927711 no 1938 0.444954114265776 1474 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress double-strand break repair|postreplication repair|protein polyubiquitination|free ubiquitin chain polymerization four-way junction helicase activity|four-way junction DNA binding|DNA-dependent ATPase activity|Y-form DNA binding nuclear chromatin YOL020W YOL020W TAT2 hom Verified 0.167846822145856 0.433351893121333 no 1939 0.447260520497721 1460 NA FT PLASMA MEMBRANE High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance aromatic amino acid transport|tryptophan transport high-affinity tryptophan transmembrane transporter activity|aromatic amino acid transmembrane transporter activity integral to membrane|plasma membrane YGR036C YGR036C CAX4 hom Verified 0.167483714182183 0.433494730368588 no 1940 0.456345491216946 1505 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation lipid biosynthetic process|protein N-linked glycosylation dolichyldiphosphatase activity integral to endoplasmic reticulum membrane|integral to membrane YDR291W YDR291W HRQ1 hom Verified 0.167313116596698 0.433561842030828 no 1941 0.430954441074803 1407 NA FT NUCLEUS 3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) biological_process molecular_function nucleus YHR037W YHR037W PUT2 hom Verified 0.166804993341804 0.433761744808818 no 1942 0.442762090386461 1464 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism glutamate biosynthetic process|proline catabolic process to glutamate 1-pyrroline-5-carboxylate dehydrogenase activity mitochondrion|mitochondrial matrix YKL084W YKL084W HOT13 hom Verified 0.166297689160863 0.433961342254743 no 1943 0.428337231023851 1431 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc protein import into mitochondrial intermembrane space zinc ion binding mitochondrial intermembrane space YHL022C YHL022C SPO11 hom Verified 0.165982277973347 0.434085448418728 no 1944 0.402454213873088 1324 NA FT CELL CYCLE NUCLEUS|CHROMOSOME Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation meiotic DNA double-strand break formation endodeoxyribonuclease activity, producing 3'-phosphomonoesters nuclear chromosome YNL194C YNL194C YNL194C hom Verified 0.165790082012894 0.434161075756748 no 1945 0.42778122334179 1396 NA FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate ascospore formation molecular_function cell cortex|integral to membrane|endoplasmic reticulum|plasma membrane|cytoplasm YPL188W YPL188W POS5 hom Verified 0.165122191579434 0.43442390320339 no 1946 0.437307146045831 1439 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress cellular response to oxidative stress|NADP biosynthetic process NADH kinase activity mitochondrion|mitochondrial matrix YMR115W YMR115W MGR3 hom Verified 0.161662905917365 0.435785658505274 no 1947 0.433942219094178 1428 NA FT PROTEOLYSIS MITOCHONDRION Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA misfolded or incompletely synthesized protein catabolic process misfolded protein binding mitochondrion|mitochondrial inner membrane|i-AAA complex YJR049C YJR049C UTR1 hom Verified 0.161485926702746 0.435855347374787 no 1948 0.447715481333048 1488 NA FF|FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS|COFACTOR METABOLISM NUCLEUS ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication cellular iron ion homeostasis|NADP biosynthetic process NAD+ kinase activity|NADH kinase activity nucleus|cytoplasm YMR037C YMR037C MSN2 hom Verified 0.161335261096946 0.435914676339231 no 1949 0.425033829664925 1415 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RESPONSE TO OXIDATIVE STRESS NUCLEUS Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress positive regulation of transcription from RNA polymerase II promoter in response to heat stress|positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment|positive regulation of transcription from RNA polymerase II promoter in response to alkalinity|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation|positive regulation of transcription from RNA polymerase II promoter in response to acidity|chromatin remodeling|positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide|regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction|positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter in response to increased salt|positive regulation of transcription from RNA polymerase II promoter in response to freezing|positive regulation of transcription from RNA polymerase II promoter in response to ethanol|age-dependent response to oxidative stress involved in chronological cell aging|replicative cell aging|positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure|cellular response to methylmercury|positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress|positive regulation of transcription from RNA polymerase II promoter in response to cold|positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding cytosol|nucleus YOR172W YOR172W YRM1 hom Verified 0.161323271370134 0.435919397704855 no 1950 0.437089384158823 1457 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding|nucleic acid binding transcription factor activity nucleus|cytoplasm YKR103W YKR103W NFT1 hom Verified 0.161180379228746 0.435975667082882 no 1951 0.443269247808363 1458 NA FT NUCLEOTIDE METABOLISM Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. transport ATPase activity, coupled to transmembrane movement of substances integral to membrane|membrane YLR148W YLR148W PEP3 hom Verified 0.160149107725927 0.436381808993625 no 1952 0.458241730994099 1535 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis regulation of SNARE complex assembly|regulation of vacuole fusion, non-autophagic|vacuole fusion, non-autophagic|late endosome to vacuole transport|vesicle docking involved in exocytosis|Golgi to endosome transport phosphatidylinositol binding|protein binding, bridging fungal-type vacuole membrane|HOPS complex|proteasome storage granule|CORVET complex|extrinsic to vacuolar membrane YHR207C YHR207C SET5 hom Verified 0.160025946938322 0.436430317451697 no 1953 0.4411857874706 1462 NA FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus histone lysine methylation S-adenosylmethionine-dependent methyltransferase activity nuclear chromatin|nucleus|cytoplasm YOR354C YOR354C MSC6 hom Verified 0.159927387731845 0.436469136949766 no 1954 0.439974906147439 1458 NA FT CELL CYCLE MITOCHONDRION Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies reciprocal meiotic recombination molecular_function mitochondrion YNL292W YNL292W PUS4 hom Verified 0.159702396326779 0.436557756568778 no 1955 0.437322535581759 1445 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) tRNA modification pseudouridine synthase activity mitochondrion|nucleus YLR436C YLR436C ECM30 hom Verified 0.159181468512913 0.436762951840345 no 1956 0.445940770456176 1483 NA FT CELL WALL ORG/BIOGENESIS Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm fungal-type cell wall organization molecular_function cytoplasm YDR048C_d YDR048C YDR048C hom Dubious 0.159145141524676 0.436777261802237 no 1957 0.443078112092737 1479 NA Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YDR206W YDR206W EBS1 hom Verified 0.159090797124703 0.436798669354612 no 1958 0.443012000584968 1454 NA FT TRANSLATION NUCLEUS|CHROMOSOME Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors negative regulation of translation|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay molecular_function cytoplasmic mRNA processing body|cytoplasm YER068C-A_d YER068C-A YER068C-A hom Dubious 0.159040604107088 0.436818441743103 no 1959 0.440738312989346 1468 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YKL123W_d YKL123W YKL123W hom Dubious 0.158943266644124 0.436856786054734 no 1960 0.440365512137995 1476 NA Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Unknown Unknown Unknown YBR178W_d YBR178W YBR178W hom Dubious 0.15888365900055 0.436880267687616 no 1961 0.440906710902723 1441 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Unknown Unknown Unknown YDR254W YDR254W CHL4 hom Verified 0.158691544664955 0.43695595007021 no 1962 0.436477595084353 1412 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 kinetochore assembly|chromosome segregation|establishment of meiotic sister chromatid cohesion|protein localization to chromosome, centromeric region|maintenance of meiotic sister chromatid cohesion|establishment of mitotic sister chromatid cohesion structural molecule activity condensed nuclear chromosome outer kinetochore YOR052C YOR052C YOR052C hom Verified 0.157785445042544 0.437312934076806 no 1963 0.440985019122117 1449 NA FT NUCLEUS Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress biological_process molecular_function nucleus YKL148C YKL148C SDH1 hom Verified 0.15745226419043 0.437444213106104 no 1964 0.448930708241897 1493 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits cellular respiration|mitochondrial electron transport, succinate to ubiquinone|tricarboxylic acid cycle succinate dehydrogenase (ubiquinone) activity|flavin adenine dinucleotide binding mitochondrial respiratory chain complex II|mitochondrion|mitochondrial membrane YKR028W YKR028W SAP190 hom Verified 0.157316814547337 0.437497584582479 no 1965 0.437090617209838 1449 NA FT CELL CYCLE|RNA PROCESSING|SIGNALING Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication TOR signaling cascade|dephosphorylation|G1/S transition of mitotic cell cycle|tRNA wobble uridine modification molecular_function cytoplasm YDL130W-A YDL130W-A STF1 hom Verified 0.157259265272056 0.437520261175434 no 1966 0.446761677159521 1485 NA FT NUCLEOTIDE METABOLISM MITOCHONDRION Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress negative regulation of ATPase activity molecular_function mitochondrion|mitochondrial proton-transporting ATP synthase complex YER166W YER166W DNF1 hom Verified 0.157003560011869 0.437621021208286 no 1967 0.445404226288523 1424 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase intracellular protein transport|phospholipid translocation|endocytosis|establishment or maintenance of cell polarity phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism mitochondrion|integral to membrane|plasma membrane YFR033C YFR033C QCR6 hom Verified 0.156603677629956 0.437778601990781 no 1968 0.450386042431792 1462 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space mitochondrial electron transport, ubiquinol to cytochrome c|aerobic respiration ubiquinol-cytochrome-c reductase activity mitochondrion|integral to membrane|mitochondrial respiratory chain complex III YIL157C YIL157C COA1 hom Verified 0.155963125398347 0.438031043573281 no 1969 0.445143693219095 1456 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly mitochondrial respiratory chain complex IV assembly molecular_function integral to mitochondrial membrane|mitochondrion|integral to mitochondrial inner membrane YDR099W YDR099W BMH2 hom Verified 0.155524497501367 0.438203921346245 no 1970 0.445328024194125 1469 NA FT CELL CYCLE|SIGNALING|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS 14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress glycogen metabolic process|ascospore formation|Ras protein signal transduction|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|DNA damage checkpoint|DNA replication initiation|signal transduction involved in filamentous growth|pseudohyphal growth|fungal-type cell wall chitin biosynthetic process|pre-replicative complex assembly DNA replication origin binding|phosphoserine binding nucleus|plasma membrane YPR067W YPR067W ISA2 hom Verified 0.155454271788074 0.438231600720453 no 1971 0.458131740014239 1511 NA FT KETONE METABOLISM|COFACTOR METABOLISM MITOCHONDRION Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations iron-sulfur cluster assembly|biotin biosynthetic process iron ion binding mitochondrion|mitochondrial intermembrane space YMR058W YMR058W FET3 hom Verified 0.155202481939601 0.438330845847129 no 1972 0.450852233320038 1479 NA FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS PLASMA MEMBRANE Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress iron assimilation by reduction and transport|high-affinity iron ion transport|response to copper ion ferroxidase activity|iron ion transmembrane transporter activity integral to membrane|high affinity iron permease complex|plasma membrane YLR020C YLR020C YEH2 hom Verified 0.155160263472543 0.438347486997582 no 1973 0.442423596185892 1473 NA FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS PLASMA MEMBRANE Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism cell wall mannoprotein biosynthetic process|sterol metabolic process sterol esterase activity integral to membrane|plasma membrane YML030W YML030W RCF1 hom Verified 0.154911688773712 0.438445469291361 no 1974 0.441526763426544 1489 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast mitochondrial respiratory chain complex IV assembly|mitochondrial respiratory chain supercomplex assembly molecular_function mitochondrion|mitochondrial respiratory chain supercomplex|integral to mitochondrial inner membrane|mitochondrial respiratory chain complex IV YKL006W YKL006W RPL14A hom Verified 0.154909323244734 0.438446401745323 no 1975 0.450382834051455 1480 NA FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YNR065C_p YNR065C YNR065C hom Uncharacterized 0.154658277731515 0.438545361841706 no 1976 0.409898601180803 1353 NA Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene biological_process molecular_function integral to membrane YIL025C_d YIL025C YIL025C hom Dubious 0.154022325483653 0.438796066218809 no 1977 0.469432311779296 1573 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJL066C YJL066C MPM1 hom Verified 0.153885335933078 0.438850073302898 no 1978 0.444386150150264 1457 NA FT MITOCHONDRION Mitochondrial intermembrane space protein of unknown function biological_process molecular_function mitochondrion|extrinsic to membrane YIL099W YIL099W SGA1 hom Verified 0.153532795145853 0.438989065048515 no 1979 0.441035721243009 1457 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation ascospore formation|glycogen catabolic process glucan 1,4-alpha-glucosidase activity fungal-type vacuole YOR381W YOR381W FRE3 hom Verified 0.153307654243102 0.439077832409212 no 1980 0.443924147621465 1452 NA FT ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY PLASMA MEMBRANE Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels cellular iron ion homeostasis|siderophore transport ferric-chelate reductase activity integral to membrane|plasma membrane YNL325C YNL325C FIG4 hom Verified 0.15312402096157 0.439150236634115 no 1981 0.445858989545387 1493 NA FT LIPID METABOLISM Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain phosphatidylinositol dephosphorylation phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity fungal-type vacuole membrane|PAS complex|extrinsic to membrane YBR144C_d YBR144C YBR144C hom Dubious 0.153052688392885 0.439178362691376 no 1982 0.429584254363153 1425 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Unknown Unknown Unknown YMR311C YMR311C GLC8 hom Verified 0.153043272989841 0.4391820751583 no 1983 0.447905234367704 1479 NA FT SIGNALING|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|CHROMOSOME SEGREGATION NUCLEUS Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress chromosome segregation|glycogen biosynthetic process enzyme activator activity nucleus|cytoplasm YAL037C-A_p YAL037C-A YAL037C-A hom Uncharacterized 0.153015225517218 0.439193134229525 no 1984 0.440907950298364 1475 NA Putative protein of unknown function biological_process molecular_function cellular_component YDR384C YDR384C ATO3 hom Verified 0.152919013701885 0.439231070746895 no 1985 0.453344021453817 1487 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters ammonium transport|nitrogen utilization|transmembrane transport ammonium transmembrane transporter activity mitochondrion|integral to membrane|plasma membrane YBR076W YBR076W ECM8 hom Verified 0.152210353505822 0.439510514098529 no 1986 0.450001016743646 1483 NA FT CELL WALL ORG/BIOGENESIS Non-essential protein of unknown function fungal-type cell wall organization molecular_function cellular_component YNL077W YNL077W APJ1 hom Verified 0.152019485134062 0.439585783675461 no 1987 0.449715948187557 1496 NA FT NUCLEUS|MITOCHONDRION Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress biological_process unfolded protein binding mitochondrion|nucleus|cytoplasm YER067C-A_d YER067C-A YER067C-A hom Dubious 0.152014741844771 0.439587654235185 no 1988 0.437772223083573 1434 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Unknown Unknown Unknown YPL067C_p YPL067C YPL067C hom Uncharacterized 0.151833188023225 0.439659252666669 no 1989 0.428263502449998 1396 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene biological_process molecular_function cytoplasm YKR091W YKR091W SRL3 hom Verified 0.151613988219875 0.439745699976998 no 1990 0.447108238854485 1467 NA Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate nucleobase-containing compound metabolic process molecular_function cytoplasm YML083C YML083C YML083C hom Verified 0.151371792345987 0.439841219744107 no 1991 0.438067725833252 1440 NA Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions biological_process molecular_function cellular_component YOR274W YOR274W MOD5 hom Verified 0.151254990093236 0.43988728670237 no 1992 0.439618150040091 1462 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis tRNA modification tRNA dimethylallyltransferase activity cytosol|mitochondrion|nucleolus|nucleus YJR135C YJR135C MCM22 hom Verified 0.151247554607892 0.439890219294885 no 1993 0.442068441560736 1468 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 chromosome segregation|establishment of mitotic sister chromatid cohesion molecular_function condensed nuclear chromosome kinetochore YDR447C YDR447C RPS17B hom Verified 0.150956594687337 0.440004977927113 no 1994 0.425124464099245 1411 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation|ribosomal small subunit assembly structural constituent of ribosome cytosolic small ribosomal subunit YLR375W YLR375W STP3 hom Verified 0.15076740044599 0.440079601467594 no 1995 0.446229992505277 1459 NA FT NUCLEUS Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids biological_process sequence-specific DNA binding nucleus YDR129C YDR129C SAC6 hom Verified 0.150651905251484 0.440125157073591 no 1996 0.484107399678111 1603 NA FF|FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress bipolar cellular bud site selection|actin filament organization|endocytosis|response to osmotic stress actin filament binding|protein binding, bridging actin filament bundle|mating projection tip|actin cortical patch YDR477W YDR477W SNF1 hom Verified 0.150592290149179 0.440148671800335 no 1997 0.441031696625969 1457 NA FT KETONE METABOLISM|SIGNALING|TRANSLATION|CARBOHYDRATE METABOLISM|PROTEIN PHOSPHORYLATION|RESPONSE TO NUTRIENT LEVELS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation negative regulation of translation|protein phosphorylation|replicative cell aging|signal transduction|biofilm formation|positive regulation of gluconeogenesis|pseudohyphal growth|cell adhesion|regulation of carbohydrate metabolic process|invasive growth in response to glucose limitation|cellular response to nitrogen starvation AMP-activated protein kinase activity|protein kinase activity mitochondrion|AMP-activated protein kinase complex|nucleus|nuclear envelope lumen|fungal-type vacuole|cytoplasm YDR391C_p YDR391C YDR391C hom Uncharacterized 0.150491075274769 0.440188595726349 no 1998 0.443202615525924 1468 NA FT NUCLEUS Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YDR220C_d YDR220C YDR220C hom Dubious 0.150194239336743 0.44030568534288 no 1999 0.434391808863971 1449 NA Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Unknown Unknown Unknown YOR231W YOR231W MKK1 hom Verified 0.150094851656967 0.440344890877817 no 2000 0.44491505656041 1483 NA FT SIGNALING|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION SITE OF POLARIZED GROWTH MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication signal transduction|peroxisome degradation|protein phosphorylation protein kinase activity|MAP kinase kinase activity cellular bud tip YGL034C_d YGL034C YGL034C hom Dubious 0.149833530995007 0.440447977031051 no 2001 0.451480069429502 1483 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOL096C YOL096C COQ3 hom Verified 0.149774046507358 0.44047144312212 no 2002 0.440236387672516 1469 NA FT KETONE METABOLISM|COFACTOR METABOLISM MITOCHONDRION O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein ubiquinone biosynthetic process hexaprenyldihydroxybenzoate methyltransferase activity mitochondrion|mitochondrial inner membrane|extrinsic to membrane YJL138C YJL138C TIF2 hom Verified 0.149747381154296 0.440481962430015 no 2003 0.44182960007455 1452 NA FT TRANSLATION RIBOSOME Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication translational initiation|regulation of translational initiation ATP-dependent RNA helicase activity|translation initiation factor activity ribosome|eukaryotic translation initiation factor 4F complex|plasma membrane|cytoplasm YJL055W_p YJL055W YJL055W hom Uncharacterized 0.149740810495454 0.440484554518531 no 2004 0.44284676099713 1458 NA FT NUCLEUS Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU response to chemical stimulus|response to purine-containing compound molecular_function nucleus|cytoplasm YBR179C YBR179C FZO1 hom Verified 0.149544299900034 0.440562078038099 no 2005 0.440698251821687 1446 NA FT MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system mitochondrial fusion GTPase activity|protein homodimerization activity mitochondrial outer membrane|mitochondrion|mitochondrial inner membrane|integral to mitochondrial outer membrane YER015W YER015W FAA2 hom Verified 0.149503122698995 0.440578322751505 no 2006 0.445822016348414 1468 NA FT KETONE METABOLISM|LIPID METABOLISM MITOCHONDRION Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome long-chain fatty acid metabolic process long-chain fatty acid-CoA ligase activity|very long-chain fatty acid-CoA ligase activity|medium-chain fatty acid-CoA ligase activity mitochondrion|peroxisome YDR117C YDR117C TMA64 hom Verified 0.149031529746995 0.44076437681847 no 2007 0.431603086501183 1415 NA FT TRANSLATION Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity biological_process RNA binding ribosome YKR024C YKR024C DBP7 hom Verified 0.148973736251606 0.440787178557519 no 2008 0.446465620457599 1468 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YDL033C YDL033C SLM3 hom Verified 0.148575281906399 0.440944389338369 no 2009 0.440944747017777 1466 NA FT RNA PROCESSING|MITOCHONDRION ORGANIZATION MITOCHONDRION tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) mitochondrial tRNA thio-modification tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity mitochondrion YGL254W YGL254W FZF1 hom Verified 0.148562354367885 0.440949490074784 no 2010 0.448431159150459 1480 NA FT TRANSCRIPTION FROM RNA POL II|ION HOMEOSTASIS|ION HOMEOSTASIS NUCLEUS Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress positive regulation of sulfite transport by positive regulation of transcription from RNA polymerase II promoter RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nucleus YPL106C YPL106C SSE1 hom Verified 0.148182311235115 0.441099445643238 no 2011 0.457706570899885 1506 NA ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication protein folding|protein refolding peptide binding|adenyl-nucleotide exchange factor activity|ATP binding polysome|cytoplasm YEL042W YEL042W GDA1 hom Verified 0.148022390041545 0.441162549094699 no 2012 0.444009561872565 1463 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate protein glycosylation guanosine-diphosphatase activity|uridine-diphosphatase activity Golgi apparatus YNL230C YNL230C ELA1 hom Verified 0.147895621795523 0.44121257175543 no 2013 0.448059581010598 1479 NA FT PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex protein ubiquitination involved in ubiquitin-dependent protein catabolic process ubiquitin-protein ligase activity elongin complex|Cul3-RING ubiquitin ligase complex YBR061C YBR061C TRM7 hom Verified 0.147893261441339 0.441213503158427 no 2014 0.440513363058333 1448 NA FT RNA PROCESSING|TRANSLATION 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively cytoplasmic translation|tRNA methylation tRNA methyltransferase activity cytoplasm YOL089C YOL089C HAL9 hom Verified 0.147845163971365 0.441232482638342 no 2015 0.382832224523717 1272 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS|MITOCHONDRION Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication response to salt stress|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding mitochondrion|nucleus YPR083W YPR083W MDM36 hom Verified 0.147783302088666 0.441256893816401 no 2016 0.451485431458722 1466 NA FT MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission mitochondrial fission|mitochondrion organization molecular_function mitochondrion|cytoplasm YHR127W YHR127W YHR127W hom Verified 0.147732726072418 0.441276851669961 no 2017 0.442354834192452 1450 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis mitotic spindle elongation molecular_function nucleus YPL104W YPL104W MSD1 hom Verified 0.147709628095908 0.441285966435729 no 2018 0.45165586141244 1502 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene mitochondrial aspartyl-tRNA aminoacylation aspartate-tRNA ligase activity mitochondrion YCR073W-A YCR073W-A SOL2 hom Verified 0.147694264761027 0.441292029027255 no 2019 0.445842734306379 1483 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|RNA LOCALIZATION|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication tRNA export from nucleus molecular_function|6-phosphogluconolactonase activity cytosol|cytoplasm YDL188C YDL188C PPH22 hom Verified 0.147690793492813 0.441293398841211 no 2020 0.439859463581913 1449 NA FT CELL CYCLE|TRANSLATION|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication actin filament organization|budding cell bud growth|G1/S transition of mitotic cell cycle|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|regulation of translation protein serine/threonine phosphatase activity condensed nuclear chromosome, centromeric region YOR289W_p YOR289W YOR289W hom Uncharacterized 0.147391137910913 0.441411650026351 no 2021 0.441999519210024 1445 NA FT NUCLEUS Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus biological_process molecular_function nucleus|cytoplasm YDR450W YDR450W RPS18A hom Verified 0.147386734974765 0.441413387568128 no 2022 0.445624414578208 1456 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION MITOCHONDRION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit|mitochondrion YDL230W YDL230W PTP1 hom Verified 0.147310437507694 0.441443497208673 no 2023 0.438363721711946 1441 NA FT MITOCHONDRION Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation pseudohyphal growth|invasive growth in response to glucose limitation|protein dephosphorylation protein tyrosine phosphatase activity mitochondrion|cytoplasm YDR252W YDR252W BTT1 hom Verified 0.147020964036969 0.441557736608641 no 2024 0.445203130119955 1474 NA FT PROTEIN LOCALIZATION NUCLEUS Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 'de novo' cotranslational protein folding unfolded protein binding nascent polypeptide-associated complex|CCR4-NOT complex YKR039W YKR039W GAP1 hom Verified 0.147010526619257 0.441561855779348 no 2025 0.436992611824954 1427 NA FT KETONE METABOLISM|AMINO ACID METABOLISM PLASMA MEMBRANE General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth amino acid transport|gamma-aminobutyric acid transport|ammonia assimilation cycle|polyamine transport amino acid transmembrane transporter activity|polyamine transmembrane transporter activity|L-proline transmembrane transporter activity endosome|fungal-type vacuole lumen|integral to membrane|plasma membrane|ER to Golgi transport vesicle|integral to plasma membrane|multivesicular body YOR302W YOR302W YOR302W hom Verified 0.146821582947847 0.441636424284681 no 2026 0.440829039928161 1469 NA FT TRANSLATION CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA regulation of translation translation regulator activity cytosol YLL047W_d YLL047W YLL047W hom Dubious 0.146530525409082 0.441751297093527 no 2027 0.44994184878301 1490 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Unknown Unknown Unknown YMR254C_d YMR254C YMR254C hom Dubious 0.14645768197861 0.441780047259928 no 2028 0.443709743723831 1472 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YLR404W YLR404W FLD1 hom Verified 0.146349537645293 0.441822730708011 no 2029 0.441695159869095 1437 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy lipid particle organization molecular_function integral to membrane|endoplasmic reticulum|cortical endoplasmic reticulum YDR018C_p YDR018C YDR018C hom Uncharacterized 0.14634594192403 0.441824149913551 no 2030 0.439442555004136 1468 NA FT LIPID METABOLISM Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication phospholipid biosynthetic process transferase activity, transferring acyl groups integral to membrane YGR189C YGR189C CRH1 hom Verified 0.146333944185438 0.441828885340819 no 2031 0.438157741409938 1458 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress fungal-type cell wall organization|cell wall chitin metabolic process transferase activity, transferring glycosyl groups incipient cellular bud site|fungal-type cell wall YMR274C YMR274C RCE1 hom Verified 0.14619370535915 0.441884237285016 no 2032 0.437714350328134 1427 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone CAAX-box protein processing|peptide pheromone maturation|protein processing metalloendopeptidase activity integral to endoplasmic reticulum membrane|integral to membrane YFR055W_p YFR055W IRC7 hom Uncharacterized 0.146135596830408 0.441907172921087 no 2033 0.433407761237805 1404 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner sulfur compound metabolic process|L-cysteine catabolic process to pyruvate cysteine-S-conjugate beta-lyase activity cellular_component YPR174C YPR174C YPR174C hom Verified 0.145932943280937 0.441987162497337 no 2034 0.434713126957766 1453 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication biological_process molecular_function nuclear envelope|spindle pole body YJL063C YJL063C MRPL8 hom Verified 0.145789581465163 0.442043750405574 no 2035 0.447952773984699 1513 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit mitochondrial genome maintenance|mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YDL018C YDL018C ERP3 hom Verified 0.145591620897724 0.442121891527395 no 2036 0.445922098408021 1468 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport vesicle-mediated transport molecular_function integral to membrane YNR041C YNR041C COQ2 hom Verified 0.145419840452306 0.442189700375405 no 2037 0.443433186290545 1468 NA FT KETONE METABOLISM|LIPID METABOLISM|COFACTOR METABOLISM MITOCHONDRION Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis ubiquinone biosynthetic process 4-hydroxybenzoate octaprenyltransferase activity mitochondrion|integral to mitochondrial membrane|integral to membrane YNL087W YNL087W TCB2 hom Verified 0.145417399929138 0.442190663763067 no 2038 0.438689627699612 1443 NA FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud biological_process lipid binding cellular bud|cortical endoplasmic reticulum YGL166W YGL166W CUP2 hom Verified 0.145402818367383 0.442196419788768 no 2039 0.449894269381845 1470 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter|response to copper ion RNA polymerase II core promoter proximal region sequence-specific DNA binding|copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity|copper ion binding nucleus YBR145W YBR145W ADH5 hom Verified 0.14534933126165 0.442217533760223 no 2040 0.442910689075878 1456 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM NUCLEUS Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication NADH oxidation|ethanol biosynthetic process involved in glucose fermentation to ethanol|amino acid catabolic process to alcohol via Ehrlich pathway alcohol dehydrogenase (NAD) activity nucleus|cytoplasm YOR190W YOR190W SPR1 hom Verified 0.145176145977898 0.442285899571168 no 2041 0.441478945241217 1475 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance ascospore formation glucan exo-1,3-beta-glucosidase activity fungal-type cell wall|ascospore wall YNR030W YNR030W ALG12 hom Verified 0.144956952852976 0.442372429686124 no 2042 0.468595628946074 1556 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation dolichol-linked oligosaccharide biosynthetic process|protein glycosylation alpha-1,6-mannosyltransferase activity integral to membrane|endoplasmic reticulum YGR161W-C_p YGR161W-C YGR161W-C hom Uncharacterized 0.144943362724993 0.442377794704699 no 2043 0.43110393625346 1457 NA Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species biological_process molecular_function cellular_component YGL251C YGL251C HFM1 hom Verified 0.144873713308331 0.442405290594984 no 2044 0.448757899177919 1469 NA FT CELL CYCLE NUCLEUS Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity meiosis|synapsis|DNA unwinding involved in replication|reciprocal meiotic recombination DNA helicase activity nucleus YOR038C YOR038C HIR2 hom Verified 0.144766512046857 0.442447611579456 no 2045 0.440311772918754 1468 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p DNA replication-independent nucleosome assembly|regulation of transcription from RNA polymerase II promoter|transcription elongation from RNA polymerase II promoter nucleosome binding|transcription corepressor activity|DNA binding HIR complex|nucleus YJL176C YJL176C SWI3 hom Verified 0.144644501026242 0.442495779969511 no 2046 0.449206027503975 1516 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS NUCLEUS RSC COMPLEX Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions cellular alcohol catabolic process|ATP-dependent chromatin remodeling|positive regulation of mating type switching|sucrose catabolic process|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter histone binding|DNA binding SWI/SNF complex|nucleus YGR045C_d YGR045C YGR045C hom Dubious 0.144581733446056 0.44252056013838 no 2047 0.439474577569243 1455 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YJR043C YJR043C POL32 hom Verified 0.144537516380422 0.442538016838553 no 2048 0.450395648739507 1492 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p RNA-dependent DNA replication|lagging strand elongation|postreplication repair|base-excision repair|DNA replication, removal of RNA primer|nucleotide-excision repair|mismatch repair|double-strand break repair via break-induced replication|leading strand elongation DNA-directed DNA polymerase activity|single-stranded DNA specific 3'-5' exodeoxyribonuclease activity delta DNA polymerase complex|nucleus YPL229W_p YPL229W YPL229W hom Uncharacterized 0.144457774854996 0.442569498718908 no 2049 0.443372884574205 1482 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication biological_process molecular_function cytoplasm YLL061W YLL061W MMP1 hom Verified 0.14436083439866 0.442607771209645 no 2050 0.444171060036357 1454 NA FT ENDOPLASMIC RETICULUM|PLASMA MEMBRANE High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p S-methylmethionine transport S-methylmethionine transmembrane transporter activity integral to membrane|plasma membrane YDL142C YDL142C CRD1 hom Verified 0.144344314919144 0.442614293221501 no 2051 0.443664927442889 1466 NA FT LIPID METABOLISM|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis lipid biosynthetic process|cellular ion homeostasis|mitochondrial membrane organization cardiolipin synthase activity mitochondrion|integral to membrane YGR010W YGR010W NMA2 hom Verified 0.144118020778585 0.442703637375357 no 2052 0.449853160430588 1477 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication NAD biosynthetic process nicotinamide-nucleotide adenylyltransferase activity nucleus YMR259C YMR259C TRM732 hom Verified 0.143851378621644 0.44280891524059 no 2053 0.449547970182137 1479 NA Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene biological_process molecular_function cytoplasm YDR169C-A_p YDR169C-A YDR169C-A hom Uncharacterized 0.143764369663272 0.442843269712516 no 2054 0.459554433782602 1520 NA Putative protein of unknown function; identified by fungal homology and RT-PCR biological_process molecular_function cellular_component YHL017W_p YHL017W YHL017W hom Uncharacterized 0.14342782787059 0.442976153355686 no 2055 0.437905775641729 1445 NA FT GOLGI APPARATUS Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication biological_process molecular_function integral to membrane|clathrin-coated vesicle YOR026W YOR026W BUB3 hom Verified 0.142949884167671 0.443164880611612 no 2056 0.442612419018784 1465 NA FT CELL CYCLE NUCLEUS|CHROMOSOME KINETOCHORE Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p mitotic cell cycle G2/M transition decatenation checkpoint|mitotic cell cycle spindle assembly checkpoint ubiquitin binding mitotic checkpoint complex|condensed nuclear chromosome kinetochore YOR189W YOR189W IES4 hom Verified 0.142555809573228 0.443320499888292 no 2057 0.433611906961671 1428 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses response to DNA damage stimulus molecular_function nucleus|Ino80 complex YKL162C_p YKL162C YKL162C hom Uncharacterized 0.142111763338782 0.443495863341208 no 2058 0.43912841457795 1459 NA FT MITOCHONDRION Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion biological_process molecular_function mitochondrion YGL078C YGL078C DBP3 hom Verified 0.14189758367864 0.44358045147527 no 2059 0.44311039026173 1488 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA-dependent ATPase activity nucleolus|preribosome, large subunit precursor YOR338W_p YOR338W YOR338W hom Uncharacterized 0.141847936458409 0.443600059520017 no 2060 0.435291685801035 1436 NA FT TRANSCRIPTION FROM RNA POL II Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication ascospore formation|positive regulation of transcription from RNA polymerase II promoter molecular_function cellular_component YGR067C_p YGR067C YGR067C hom Uncharacterized 0.141759216194035 0.443635099709099 no 2061 0.437221805379681 1439 NA FT NUCLEUS Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p biological_process sequence-specific DNA binding cellular_component YOR324C YOR324C FRT1 hom Verified 0.141745935644678 0.443640344918059 no 2062 0.441768572978621 1455 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication response to stress molecular_function endoplasmic reticulum YBR024W YBR024W SCO2 hom Verified 0.14115806539497 0.443872536630752 no 2063 0.441772636777279 1448 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication copper ion transport thioredoxin peroxidase activity mitochondrial envelope|mitochondrion YHR007C-A_p YHR007C-A YHR007C-A hom Uncharacterized 0.141002087504046 0.443934146615297 no 2064 0.438278428814069 1444 NA Putative protein of unknown function; identified by expression profiling and mass spectrometry biological_process molecular_function cellular_component YER158C_p YER158C YER158C hom Uncharacterized 0.140903546583528 0.443973070167276 no 2065 0.441166105122775 1471 NA Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication biological_process molecular_function cellular_component YOR104W YOR104W PIN2 hom Verified 0.140836939437004 0.443999380220839 no 2066 0.44994248984321 1489 NA Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated biological_process molecular_function cellular_component YCL069W YCL069W VBA3 hom Verified 0.140562517490245 0.444107780442134 no 2067 0.446695598191497 1481 NA Permease of basic amino acids in the vacuolar membrane basic amino acid transport|transmembrane transport basic amino acid transmembrane transporter activity fungal-type vacuole membrane|integral to membrane YAL018C_p YAL018C YAL018C hom Uncharacterized 0.140418035177724 0.444164854506684 no 2068 0.450300819080544 1473 NA Putative protein of unknown function biological_process molecular_function integral to membrane YJR040W YJR040W GEF1 hom Verified 0.140350600594985 0.444191493220693 no 2069 0.4385915778544 1457 NA FF|FT ION HOMEOSTASIS|ION HOMEOSTASIS ENDOPLASMIC RETICULUM|GOLGI APPARATUS|PLASMA MEMBRANE Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism cellular copper ion homeostasis|cellular iron ion homeostasis voltage-gated chloride channel activity endosome|integral to membrane|endoplasmic reticulum|Golgi apparatus|plasma membrane|fungal-type vacuole|Golgi medial cisterna YCR064C_d YCR064C YCR064C hom Dubious 0.140177682556105 0.444259802264406 no 2070 0.454344847454289 1503 NA FF Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Unknown Unknown Unknown YCR065W YCR065W HCM1 hom Verified 0.140131135328836 0.444278190429842 no 2071 0.441832966494835 1474 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO OXIDATIVE STRESS NUCLEUS|CHROMOSOME Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role positive regulation of transcription involved in S-phase of mitotic cell cycle|cellular response to oxidative stress|mitochondrion organization|spindle pole body organization|positive regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nuclear chromatin|nucleus|cytoplasm YJL185C YJL185C ATG36 hom Verified 0.140118076887989 0.444283349099598 no 2072 0.438490538936302 1457 NA Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene peroxisome degradation molecular_function peroxisome YML067C YML067C ERV41 hom Verified 0.139996334026622 0.44433144344363 no 2073 0.446535836588085 1471 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein ER to Golgi vesicle-mediated transport molecular_function integral to endoplasmic reticulum membrane|integral to Golgi membrane|ER to Golgi transport vesicle YCL034W YCL034W LSB5 hom Verified 0.139502117529309 0.444526691377876 no 2074 0.450526355315448 1485 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat endocytosis|actin filament organization|actin cortical patch localization|actin cytoskeleton organization molecular_function cell cortex|cytoplasm|actin cortical patch YJL094C YJL094C KHA1 hom Verified 0.139382371647387 0.444574000883655 no 2075 0.442971620073379 1447 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION|GOLGI APPARATUS Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies monovalent inorganic cation transport potassium:hydrogen antiporter activity mitochondrion|integral to membrane|Golgi apparatus YNR061C YNR061C YNR061C hom Verified 0.138921793761501 0.444755974512799 no 2076 0.434572470015467 1437 NA Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress biological_process molecular_function integral to membrane|fungal-type vacuole YGR210C_p YGR210C YGR210C hom Uncharacterized 0.138831195675361 0.444791771051865 no 2077 0.437345240011749 1424 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm biological_process molecular_function cytoplasm YER058W YER058W PET117 hom Verified 0.138658006345466 0.444860201770179 no 2078 0.457689478499068 1502 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Protein required for assembly of cytochrome c oxidase mitochondrial respiratory chain complex IV assembly molecular_function mitochondrion YHL046C YHL046C PAU13 hom Verified 0.13860725331293 0.444880255663875 no 2079 0.437898511215522 1446 NA Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock biological_process molecular_function cellular_component YER151C YER151C UBP3 hom Verified 0.138578153362444 0.444891753903766 no 2080 0.45187713077509 1493 NA FT VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress protein deubiquitination|ribophagy|regulation of ER to Golgi vesicle-mediated transport|regulation of response to osmotic stress ubiquitin-specific protease activity|mRNA binding cytoplasm YJR094C YJR094C IME1 hom Verified 0.138383778646139 0.444968558216939 no 2081 0.436168446185088 1426 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p pseudohyphal growth|regulation of transcription from RNA polymerase II promoter|regulation of meiosis protein binding transcription factor activity nucleus YGR282C YGR282C BGL2 hom Verified 0.13813502959052 0.445066850764629 no 2082 0.448482315418494 1485 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance fungal-type cell wall organization 1,3-beta-glucanosyltransferase activity|glucan endo-1,3-beta-D-glucosidase activity fungal-type cell wall YMR262W_p YMR262W YMR262W hom Uncharacterized 0.137968686287423 0.445132582776616 no 2083 0.439187031652752 1457 NA Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene biological_process molecular_function cellular_component YJL147C_p YJL147C YJL147C hom Uncharacterized 0.137407794485217 0.445354235189944 no 2084 0.441577710324062 1464 NA FT MITOCHONDRION Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene biological_process molecular_function mitochondrion YIL056W YIL056W VHR1 hom Verified 0.137054392848265 0.445493900716346 no 2085 0.434023579036766 1425 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO NUTRIENT LEVELS NUCLEUS Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication regulation of transcription from RNA polymerase II promoter in response to biotin starvation|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II regulatory region sequence-specific DNA binding|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity nucleus|cytoplasm YMR075C-A_d YMR075C-A YMR075C-A hom Dubious 0.136941486629734 0.445538523063828 no 2086 0.429696694694094 1426 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Unknown Unknown Unknown YOR385W_p YOR385W YOR385W hom Uncharacterized 0.136632367648733 0.445660695368993 no 2087 0.44718408985681 1474 NA Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene biological_process molecular_function cytoplasm YAL060W YAL060W BDH1 hom Verified 0.136572469193604 0.445684369479568 no 2088 0.447750661860733 1481 NA FT OXIDATION-REDUCTION PROCESS NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source alcohol metabolic process|butanediol biosynthetic process (R,R)-butanediol dehydrogenase activity cytoplasm YBR037C YBR037C SCO1 hom Verified 0.136537600958911 0.445698150799705 no 2089 0.452110057805898 1496 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|ION HOMEOSTASIS|ION HOMEOSTASIS MITOCHONDRION Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication copper ion transport|response to redox state|protein complex assembly copper ion binding|thioredoxin peroxidase activity mitochondrion|mitochondrial inner membrane YOR121C_d YOR121C YOR121C hom Dubious 0.136463226015986 0.445727546969348 no 2090 0.448739220481658 1450 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Unknown Unknown Unknown YER042W YER042W MXR1 hom Verified 0.136342393240811 0.445775305904324 no 2091 0.452606264272518 1510 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS NUCLEUS Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan cellular response to oxidative stress peptide-methionine-(S)-S-oxide reductase activity nucleus|cytoplasm YDR207C YDR207C UME6 hom Verified 0.136311847602742 0.445787379136923 no 2092 0.482953854665884 1606 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS NUCLEUS|CHROMOSOME Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p spore germination|positive regulation of transcription from RNA polymerase II promoter during meiosis|negative regulation of transcription from RNA polymerase II promoter during meiosis|lipid particle organization|nitrogen catabolite repression of transcription from RNA polymerase II promoter|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter during mitosis|Unknown RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|repressing transcription factor binding|transcription factor binding transcription factor activity Rpd3L-Expanded complex|Rpd3L complex YKL007W YKL007W CAP1 hom Verified 0.136208722237009 0.445828140042479 no 2093 0.442088839865315 1444 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress barbed-end actin filament capping actin filament binding actin filament|F-actin capping protein complex|mating projection tip|actin cortical patch YER081W YER081W SER3 hom Verified 0.13609162541714 0.445874423941355 no 2094 0.445705901543744 1455 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication serine family amino acid biosynthetic process phosphoglycerate dehydrogenase activity cytoplasm YLR063W_p YLR063W YLR063W hom Uncharacterized 0.135817826355212 0.445982649126786 no 2095 0.444704526474972 1450 NA Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene biological_process S-adenosylmethionine-dependent methyltransferase activity cytoplasm YBR270C_p YBR270C BIT2 hom Uncharacterized 0.135688766806271 0.44603366418789 no 2096 0.425011785568912 1418 NA Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication biological_process molecular_function cellular_component YHR198C YHR198C AIM18 hom Verified 0.135243818983622 0.446209551395415 no 2097 0.442403895377318 1476 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss biological_process molecular_function mitochondrion YDL155W YDL155W CLB3 hom Verified 0.135106840133295 0.446263701062093 no 2098 0.449935628303595 1483 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress G2/M transition of mitotic cell cycle|S phase of mitotic cell cycle|regulation of cyclin-dependent protein kinase activity|positive regulation of spindle pole body separation cyclin-dependent protein kinase regulator activity nucleus|cytoplasm YDR006C YDR006C SOK1 hom Verified 0.134123992580143 0.446652263859321 no 2099 0.437027503536156 1460 NA FT SIGNALING NUCLEUS Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 cAMP-mediated signaling molecular_function nucleus YKR051W_p YKR051W YKR051W hom Uncharacterized 0.134052341104428 0.44668059284186 no 2100 0.448600872136554 1483 NA Putative protein of unknown function biological_process molecular_function integral to membrane YHR014W YHR014W SPO13 hom Verified 0.13404960459123 0.446681674787771 no 2101 0.434957799132793 1412 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II protein localization to chromosome, centromeric region|meiotic sister chromatid cohesion|positive regulation of sister chromatid cohesion molecular_function nucleus|condensed nuclear chromosome, centromeric region YML001W YML001W YPT7 hom Verified 0.132755947488112 0.447193197351899 no 2102 0.440409445257472 1438 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT MITOCHONDRION Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 regulation of vacuole fusion, non-autophagic|piecemeal microautophagy of nucleus|vacuole inheritance|Golgi to vacuole transport|vesicle-mediated transport|endocytosis GTPase activity mitochondrial outer membrane|fungal-type vacuole YMR264W YMR264W CUE1 hom Verified 0.132743933701814 0.447197948113973 no 2103 0.45626210457015 1512 NA FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION UBIQUITIN LIGASE COMPLEX Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication ER-associated protein catabolic process|establishment of protein localization to endoplasmic reticulum membrane ubiquitin-protein ligase activator activity Doa10p ubiquitin ligase complex|mitochondrion|integral to endoplasmic reticulum membrane YNL213C YNL213C RRG9 hom Verified 0.132558022574917 0.447271466246109 no 2104 0.444193376651728 1468 NA FT MITOCHONDRION ORGANIZATION MITOCHONDRION Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies mitochondrion organization|mitochondrial genome maintenance molecular_function mitochondrion YDR073W YDR073W SNF11 hom Verified 0.132164114557911 0.447427242258408 no 2105 0.435980306801459 1421 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RSC COMPLEX Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions positive regulation of transcription from RNA polymerase II promoter molecular_function SWI/SNF complex YJR031C YJR031C GEA1 hom Verified 0.132114569518352 0.447446836058802 no 2106 0.438448487510049 1444 NA FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS GOLGI APPARATUS Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|actin cytoskeleton organization ARF guanyl-nucleotide exchange factor activity integral to membrane|Golgi cis cisterna|Golgi-associated vesicle|extrinsic to membrane YDR395W YDR395W SXM1 hom Verified 0.131667741573854 0.447623550911012 no 2107 0.444576680258492 1492 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 mRNA export from nucleus|nucleocytoplasmic transport protein transporter activity integral to membrane|nucleus|cytoplasm YPL054W YPL054W LEE1 hom Verified 0.131635682680125 0.447636230201857 no 2108 0.421851853195092 1378 NA Zinc-finger protein of unknown function biological_process molecular_function cellular_component YNR064C YNR064C YNR064C hom Verified 0.131157879261984 0.447825207764002 no 2109 0.446305838625032 1490 NA Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides response to toxin epoxide hydrolase activity cellular_component YGR166W YGR166W TRS65 hom Verified 0.130595592679191 0.448047614699694 no 2110 0.474887462312945 1580 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS GOLGI APPARATUS Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in