strainid orf gene zyg qualifier SGTC_2053|5265679.score SGTC_2053|5265679.expt.zyg.pval SGTC_2053|5265679.expt.zyg.significant SGTC_2053|5265679.expt.zyg.rank strain.pval strain.rank clearance Fclass Fterms.BP Fterms.CC Fterms.complex description GO_process GO_function GO_component YDL014W YDL014W NOP1 het Verified 5.58928883825787 1.14000717492944e-08 yes 1 8.80480930905975e-08 3 0.787 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin box C/D snoRNA 3'-end processing|rRNA processing|snoRNA processing|rRNA methylation rRNA methyltransferase activity small-subunit processome|box C/D snoRNP complex|ribosome|nucleolus|90S preribosome YOL040C YOL040C RPS15 het Verified 5.17891473972248 1.11590217262375e-07 yes 2 1.72246346412467e-05 11 0.787 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome cytosolic small ribosomal subunit YDL111C YDL111C RRP42 het Verified 5.09273738429616 1.76465200911484e-07 yes 3 1.44994743190014e-06 11 0.787 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) nuclear polyadenylation-dependent tRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YGR073C_d YGR073C YGR073C het Dubious 5.00043214266317 2.86009790571097e-07 yes 4 1.22715522208567e-06 6 0.787 Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Unknown Unknown Unknown YJL086C_d YJL086C YJL086C het Dubious 4.75084388053368 1.01284743492326e-06 yes 5 0.00010997669021385 60 0.787 FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Unknown Unknown Unknown YBR152W YBR152W SPP381 het Verified 3.96400240356958 3.6851754818159e-05 yes 6 8.59776309911154e-05 19 0.134 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step molecular_function U4/U6 x U5 tri-snRNP complex YBL092W YBL092W RPL32 het Verified 3.82974044465749 6.41392447537961e-05 yes 7 0.00172032550997891 104 0.056 FF|FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YJR007W YJR007W SUI2 het Verified 3.77395173848768 8.03409290727718e-05 yes 8 0.00237738680001963 77 0.186 FF|FT TRANSLATION RIBOSOME Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress translational initiation translation initiation factor activity ribosome|multi-eIF complex|eukaryotic translation initiation factor 2 complex|cytoplasm YLR195C YLR195C NMT1 het Verified 3.58746304654689 0.000166955518826911 yes 9 3.74723628379887e-05 10 0.149 N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction N-terminal peptidyl-glycine N-myristoylation|replicative cell aging glycylpeptide N-tetradecanoyltransferase activity cytosol YDR164C YDR164C SEC1 het Verified 3.43830334806146 0.000292685770350444 yes 10 0.000273902935514967 24 0.173 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function positive regulation of vesicle fusion|exocytosis|vesicle docking involved in exocytosis SNARE binding plasma membrane|cellular bud neck|cellular bud tip YDR429C YDR429C TIF35 het Verified 3.26494193642911 0.000547432588823125 yes 11 0.0131072341002884 113 0.015 FF|FT TRANSLATION eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity eukaryotic translation initiation factor 3 complex YGR128C YGR128C UTP8 het Verified 3.24975760273805 0.000577517071862182 yes 12 0.00182090561566808 50 0.137 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION NUCLEUS|CHROMOSOME Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|tRNA export from nucleus snoRNA binding|tRNA binding small-subunit processome|t-UTP complex|nucleolus|rDNA heterochromatin|90S preribosome YGL128C YGL128C CWC23 het Verified 3.11280479641146 0.000926592729753554 yes 13 0.000244966574639658 23 0.076 FT RNA PROCESSING NUCLEUS Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p spliceosomal complex disassembly molecular_function U2-type spliceosomal complex YHR007C YHR007C ERG11 het Verified 3.03636788049975 0.00119723510269128 no 14 0.0137442091082988 280 0.042 FF|FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOPLASMIC RETICULUM Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p ergosterol biosynthetic process sterol 14-demethylase activity endoplasmic reticulum YDR177W YDR177W UBC1 het Verified 2.99431609489901 0.00137530409409597 no 15 0.00345689538441043 63 0.069 FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|PROTEOLYSIS Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress spore germination|vesicle organization|endocytosis|ER-associated protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|protein polyubiquitination|protein monoubiquitination ubiquitin-protein ligase activity proteasome complex YPL238C_d YPL238C YPL238C het Dubious 2.92566927908492 0.00171858060318982 no 16 0.0122905458204189 140 0.027 FF Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Unknown Unknown Unknown YNL110C YNL110C NOP15 het Verified 2.89855384389279 0.00187443966469963 no 17 0.0094925239753744 111 0.115 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm cytokinesis, actomyosin contractile ring assembly|ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YOL077C YOL077C BRX1 het Verified 2.78364861496055 0.00268756156916465 no 18 0.00180674518633108 67 0.062 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif ribosomal large subunit assembly 5S rRNA binding|rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YOR217W YOR217W RFC1 het Verified 2.72170240427699 0.00324732997695851 no 19 0.00670237246120695 79 0.041 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION NUCLEUS|CHROMOSOME Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon DNA repair|mismatch repair|mitotic cell cycle|leading strand elongation DNA clamp loader activity DNA replication factor C complex YIL078W YIL078W THS1 het Verified 2.6806695586269 0.00367375151821577 no 20 0.00203719147309628 56 0.007 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Threonyl-tRNA synthetase, essential cytoplasmic protein cytoplasmic translation|threonyl-tRNA aminoacylation threonine-tRNA ligase activity mitochondrion|cytoplasm YJL143W YJL143W TIM17 het Verified 2.67378868540504 0.00374998468233398 no 21 0.00340008614437798 71 0.047 FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex protein import into mitochondrial matrix|mitochondrial genome maintenance protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane presequence translocase complex YPR178W YPR178W PRP4 het Verified 2.62671898893907 0.00431062492083882 no 22 0.00174062356381306 51 0.009 FT RNA PROCESSING NUCLEUS Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YLR086W YLR086W SMC4 het Verified 2.6178220946876 0.00442464641597313 no 23 0.00746016079915967 73 0.062 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate mitotic chromosome condensation|mitotic sister chromatid segregation|tRNA gene clustering chromatin binding|ATPase activity SMC loading complex|nuclear condensin complex YPL237W YPL237W SUI3 het Verified 2.55604713808001 0.00529343880438783 no 24 0.0176022892735799 150 0.003 FT TRANSLATION RIBOSOME Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding translational initiation translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YGR119C YGR119C NUP57 het Verified 2.55340666161005 0.00533374329800204 no 25 0.0180117833782825 143 0.001 FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) NLS-bearing substrate import into nucleus|protein import into nucleus|nuclear pore organization|protein targeting to nuclear inner membrane nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore YDR182W YDR182W CDC1 het Verified 2.55232002531439 0.00535040892524779 no 26 0.00153179617171128 57 0.031 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS ENDOPLASMIC RETICULUM Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution DNA repair|cellular manganese ion homeostasis|dephosphorylation|actin cytoskeleton organization molecular_function integral to membrane|endoplasmic reticulum YDL126C YDL126C CDC48 het Verified 2.52122510040981 0.00584735066543094 no 27 0.00499143020244471 109 0.028 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION|SITE OF POLARIZED GROWTH UBIQUITIN LIGASE COMPLEX AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP proteasomal ubiquitin-dependent protein catabolic process|mitotic spindle disassembly|ER-associated protein catabolic process|mitochondria-associated protein catabolic process|macroautophagy|sister chromatid biorientation|ER-associated misfolded protein catabolic process|nucleus-associated proteasomal ubiquitin-dependent protein catabolic process|retrograde protein transport, ER to cytosol|piecemeal microautophagy of nucleus|endoplasmic reticulum membrane fusion|ribophagy|Unknown|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process ubiquitin binding|ATPase activity mitochondrion|Cdc48p-Npl4p-Ufd1p AAA ATPase complex|Cdc48p-Npl4p-Vms1p AAA ATPase complex|cytosol|Doa10p ubiquitin ligase complex|nucleus|endoplasmic reticulum membrane|mating projection tip YPL016W YPL016W SWI1 het Verified 2.49316055720821 0.00633057897722782 no 28 0.0141128497040942 100 0.038 FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS NUCLEUS RSC COMPLEX Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer cellular alcohol catabolic process|ATP-dependent chromatin remodeling|positive regulation of mating type switching|sucrose catabolic process|carbon catabolite activation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation|positive regulation of transcription from RNA polymerase II promoter|DNA-dependent DNA replication RNA polymerase II activating transcription factor binding SWI/SNF complex|nucleus YPR176C YPR176C BET2 het Verified 2.45466639961333 0.00705076919065026 no 29 0.00149330107683153 43 0.016 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YJL167W YJL167W ERG20 het Verified 2.43863837882516 0.00737135649796493 no 30 0.00480633007369805 52 0.013 FT LIPID METABOLISM ENDOPLASMIC RETICULUM Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis farnesyl diphosphate biosynthetic process|ergosterol biosynthetic process|isoprenoid biosynthetic process dimethylallyltranstransferase activity|geranyltranstransferase activity endoplasmic reticulum|cytoplasm YHR062C YHR062C RPP1 het Verified 2.42568282778649 0.00763980869725362 no 31 0.141123037531386 611 0.014 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YDL152W_d YDL152W YDL152W het Dubious 2.41188393400764 0.00793516638830314 no 32 0.0257192550369127 195 0.005 FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Unknown Unknown Unknown YNL158W YNL158W PGA1 het Verified 2.40729329521227 0.00803562858511384 no 33 0.0040150306769192 60 0.002 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes GPI anchor biosynthetic process mannosyltransferase activity integral to endoplasmic reticulum membrane|nuclear envelope|mannosyltransferase complex YMR208W YMR208W ERG12 het Verified 2.4052016273544 0.00808177253992466 no 34 0.0203964041431207 203 0.066 FF|FT LIPID METABOLISM|COFACTOR METABOLISM Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate ergosterol biosynthetic process|isopentenyl diphosphate biosynthetic process, mevalonate pathway|farnesyl diphosphate biosynthetic process, mevalonate pathway mevalonate kinase activity cytosol YPR169W YPR169W JIP5 het Verified 2.33954711050595 0.00965356819834573 no 35 0.0125457660149682 104 0.021 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein ribosomal large subunit biogenesis molecular_function nucleolus|nucleus YJL026W YJL026W RNR2 het Verified 2.31884373746979 0.0102017553509416 no 36 0.00985780857614217 97 0.003 FT OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM NUCLEUS Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits deoxyribonucleotide biosynthetic process ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor ribonucleoside-diphosphate reductase complex|nucleus|cytoplasm YGR098C YGR098C ESP1 het Verified 2.31549506296074 0.0102929272177214 no 37 0.0237988630067961 172 0.004 FT CELL CYCLE|LIPID METABOLISM|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CYTOSKELETON Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress regulation of exit from mitosis|regulation of mitotic spindle elongation|negative regulation of protein phosphatase type 2A activity|apoptotic process|mitotic sister chromatid segregation cysteine-type endopeptidase activity mitochondrion|nucleus|cytoplasm|spindle YDR167W YDR167W TAF10 het Verified 2.31166699158186 0.0103980208762458 no 38 0.0206182788289104 136 0.017 FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|protein complex scaffold SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YDR288W YDR288W NSE3 het Verified 2.29429009674864 0.0108869205218521 no 39 0.0226814576234742 119 0.013 FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress DNA repair DNA binding Smc5-Smc6 complex|nucleus|cytoplasm YER148W YER148W SPT15 het Verified 2.28138270166458 0.0112629053727948 no 40 0.0172021822406757 122 0.069 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability transposon integration|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|RNA polymerase II transcriptional preinitiation complex assembly|RNA polymerase III transcriptional preinitiation complex assembly RNA polymerase III regulatory region DNA binding|RNA polymerase I transcription factor recruiting transcription factor activity|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding|DNA binding, bending|sequence-specific DNA binding|RNA polymerase II core promoter sequence-specific DNA binding|TFIIIB-type transcription factor activity|chromatin binding|RNA polymerase I transcription factor binding transcription factor TFIIIB complex|RNA polymerase I upstream activating factor complex|nucleus|RNA polymerase I core factor complex|transcription factor TFIID complex YGR116W YGR116W SPT6 het Verified 2.21261889979643 0.0134619654524782 no 41 0.0273665254236362 192 0.021 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|CHROMATIN ORGANIZATION|RESPONSE TO NUTRIENT LEVELS|RNA LOCALIZATION NUCLEUS|MITOCHONDRION|CHROMOSOME Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region poly(A)+ mRNA export from nucleus|regulation of transcription by chromatin organization|regulation of mRNA 3'-end processing|transcription antitermination|positive regulation of transcription involved in G1/S phase of mitotic cell cycle|regulation of transcriptional start site selection at RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter by glucose|negative regulation of transcription from RNA polymerase II promoter|nucleosome organization|chromatin maintenance|regulation of nucleosome density|nucleosome assembly|chromatin remodeling|regulation of histone H3-K36 methylation|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter in response to stress core RNA polymerase II binding transcription factor activity|nucleosome binding|histone binding|DNA binding transcription antitermination factor activity mitochondrion|nucleus|transcriptionally active chromatin YLR208W YLR208W SEC13 het Verified 2.19148973136005 0.0142081860127293 no 42 0.064930582938726 439 0.043 FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM NUCLEAR PORE Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress positive regulation of transcription, DNA-dependent|ER-associated protein catabolic process|positive regulation of GTPase activity|nuclear pore distribution|COPII-coated vesicle budding structural molecule activity COPII vesicle coat|nuclear pore outer ring|Seh1-associated complex YJL085W YJL085W EXO70 het Verified 2.14834977528937 0.015842989736521 no 43 0.0149194107385187 150 0.022 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Golgi to plasma membrane transport|exocytosis|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YPR094W YPR094W RDS3 het Verified 2.12683927250203 0.0167167199955438 no 44 0.0169222326809052 133 0.022 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance spliceosomal complex assembly|response to xenobiotic stimulus|mRNA splicing, via spliceosome molecular_function U2 snRNP YHR118C YHR118C ORC6 het Verified 2.10494423043717 0.0176480814679101 no 45 0.0116008276890009 106 0.002 FT GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p DNA replication initiation|pre-replicative complex assembly|chromatin silencing at silent mating-type cassette DNA replication origin binding pre-replicative complex|DNA replication preinitiation complex|nuclear origin of replication recognition complex YBR253W YBR253W SRB6 het Verified 2.10315655674262 0.0177260432189684 no 46 0.019706595507514 139 0.002 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YAL032C YAL032C PRP45 het Verified 2.10141514848678 0.0178022696604161 no 47 0.0152368243607945 130 0.008 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene generation of catalytic spliceosome for second transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity nucleus|post-mRNA release spliceosomal complex|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YKL153W_d YKL153W YKL153W het Dubious 2.09294596341785 0.0181769892741719 no 48 0.0175103229133989 141 0.001 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Unknown Unknown Unknown YLR076C_d YLR076C YLR076C het Dubious 2.09208506036953 0.0182154536643131 no 49 0.0492817351446306 212 0.017 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Unknown Unknown Unknown YDR280W YDR280W RRP45 het Verified 2.07552820906122 0.0189688036191509 no 50 0.0583337256817849 293 0.006 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|U1 snRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YBL076C YBL076C ILS1 het Verified 2.06913641654742 0.0192666436384499 no 51 0.0180679359214947 115 0.014 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A cytoplasmic translation|isoleucyl-tRNA aminoacylation isoleucine-tRNA ligase activity cytosol YLR051C YLR051C FCF2 het Verified 2.05525734981318 0.0199270772284913 no 52 0.0298833891856927 193 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus YJR123W YJR123W RPS5 het Verified 2.05381728986289 0.0199966894375642 no 53 0.0164289789310279 157 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION RIBOSOME Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 cytoplasmic translation|rRNA export from nucleus|regulation of translational fidelity structural constituent of ribosome cytosolic small ribosomal subunit|90S preribosome YLL034C YLL034C RIX7 het Verified 2.05377021374018 0.0199989685657684 no 54 0.00986608090575052 87 0.033 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus ATPase activity nucleolus|nucleus|preribosome, large subunit precursor YDR531W YDR531W CAB1 het Verified 2.020987930044 0.0216405072817245 no 55 0.0162199611805336 84 0.008 FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element coenzyme A biosynthetic process pantothenate kinase activity nucleus|cytoplasm YER126C YER126C NSA2 het Verified 2.01316422758491 0.0220486775946181 no 56 0.0182006887315524 150 0.027 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleus|preribosome, large subunit precursor YCL052C YCL052C PBN1 het Verified 1.98661698407805 0.0234824288215703 no 57 0.0264588663091058 173 0.000 FT LIPID METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X GPI anchor biosynthetic process|ER-associated protein catabolic process|protein processing mannosyltransferase activity endoplasmic reticulum YBR154C YBR154C RPB5 het Verified 1.98643473394249 0.0234925365375572 no 58 0.026382527479369 208 0.031 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YNL126W YNL126W SPC98 het Verified 1.95579356891206 0.0252447383911088 no 59 0.0513872603097758 287 0.015 FF|FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation|mitotic spindle organization in nucleus structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YNL267W YNL267W PIK1 het Verified 1.94104953386905 0.0261261347313304 no 60 0.0528378671299941 286 0.031 FF|FT LIPID METABOLISM|SIGNALING NUCLEUS|GOLGI APPARATUS Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity trans-Golgi network|nucleus YHR023W YHR023W MYO1 het Verified 1.90969627453801 0.0280861654679489 no 61 0.0225534044358069 145 0.014 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively cytokinesis, actomyosin contractile ring assembly|cytokinesis, completion of separation|actomyosin contractile ring contraction|cytokinesis microfilament motor activity cellular bud neck contractile ring|incipient cellular bud site|cellular bud neck|myosin II complex YDR308C YDR308C SRB7 het Verified 1.89533728444411 0.0290238658117391 no 62 0.0194978671084485 134 0.019 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity|RNA polymerase II repressing transcription factor binding|RNA polymerase II transcription corepressor activity core mediator complex YDR180W YDR180W SCC2 het Verified 1.87588427400515 0.0303355862547993 no 63 0.0257732426506009 152 0.003 FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin double-strand break repair|establishment of protein localization to chromatin|protein localization to chromatin|2-micrometer plasmid partitioning|mitotic chromosome condensation|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery|establishment of mitotic sister chromatid cohesion|rDNA condensation|tRNA gene clustering molecular_function nuclear chromatin|2-micrometer circle DNA|SMC loading complex YPL242C YPL242C IQG1 het Verified 1.8727139240671 0.0305539497785737 no 64 0.0274633247199102 153 0.012 FT CELL CYCLE|SIGNALING|CELL DIVISION|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress actin filament organization|response to osmotic stress|cytokinesis cytoskeletal protein binding cellular bud neck contractile ring YLR071C YLR071C RGR1 het Verified 1.86022225052295 0.0314270441406407 no 65 0.0252673586624379 106 0.001 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II repressing transcription factor binding core mediator complex YIL062C YIL062C ARC15 het Verified 1.85886416719431 0.0315231971577189 no 66 0.0220909804663182 167 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION MITOCHONDRION|CYTOSKELETON Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity mitochondrion inheritance|actin cortical patch assembly mRNA binding|structural molecule activity Arp2/3 protein complex|extrinsic to mitochondrial outer membrane YOR341W YOR341W RPA190 het Verified 1.85354505610064 0.03190213722179 no 67 0.0283285068718693 190 0.008 FT NUCLEUS RNA polymerase I largest subunit A190 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|nucleolus YJL087C YJL087C TRL1 het Verified 1.84546017030206 0.0324853136420716 no 68 0.0146167462464963 98 0.001 FT RNA PROCESSING|SIGNALING|TRANSLATION ENDOMEMBRANE SYSTEM|NUCLEUS tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response|tRNA splicing, via endonucleolytic cleavage and ligation|positive regulation of translation in response to stress GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity|RNA ligase (ATP) activity|2',3'-cyclic-nucleotide 3'-phosphodiesterase activity nuclear inner membrane|polysome YGL099W YGL099W LSG1 het Verified 1.84431381596386 0.0325687095827125 no 69 0.0757758016597438 537 0.003 FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS SITE OF POLARIZED GROWTH Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm conjugation with cellular fusion|ribosomal large subunit assembly|ribosomal subunit export from nucleus|ascospore formation GTPase activity mating projection tip|cytoplasm YDR045C YDR045C RPC11 het Verified 1.84090399671945 0.0328178143095283 no 70 0.0404481177452448 214 0.001 FT NUCLEUS RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS termination of RNA polymerase III transcription|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YEL032W YEL032W MCM3 het Verified 1.84003313618709 0.0328816863114405 no 71 0.0472099278406215 218 0.018 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|double-strand break repair via break-induced replication|DNA replication initiation|pre-replicative complex assembly|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette DNA helicase activity|chromatin binding|DNA replication origin binding|ATPase activity replication fork protection complex|DNA replication preinitiation complex|pre-replicative complex|nucleus|cytoplasm|MCM complex YLR409C YLR409C UTP21 het Verified 1.82226983840177 0.0342070261716416 no 72 0.0429479571856313 187 0.009 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma ribosomal small subunit biogenesis|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YBR110W YBR110W ALG1 het Verified 1.8132926061992 0.0348933515407636 no 73 0.0264631662489821 168 0.017 FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog oligosaccharide-lipid intermediate biosynthetic process|protein N-linked glycosylation beta-1,4-mannosyltransferase activity integral to membrane|endoplasmic reticulum YPL124W YPL124W SPC29 het Verified 1.79664098175996 0.0361963169674024 no 74 0.0222490767505731 156 0.009 FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication microtubule nucleation|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton central plaque of spindle pole body YDR381W YDR381W YRA1 het Verified 1.78782104740448 0.0369024420116283 no 75 0.0369615794714988 195 0.023 FT RESPONSE TO DNA DAMAGE|RNA LOCALIZATION NUCLEUS RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member mRNA export from nucleus|transcription-coupled nucleotide-excision repair RNA binding transcription export complex YKR086W YKR086W PRP16 het Verified 1.76522621839363 0.0387628769423774 no 76 0.0295545523240276 151 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity|RNA-dependent ATPase activity U2-type catalytic step 2 spliceosome YGR013W YGR013W SNU71 het Verified 1.76480789988601 0.0387980282561948 no 77 0.0339317317906484 189 0.026 FT RNA PROCESSING NUCLEUS Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart mRNA splicing, via spliceosome RNA binding U1 snRNP|U2-type prespliceosome YNR054C YNR054C ESF2 het Verified 1.73832080027946 0.041077151633381 no 78 0.033964712972708 159 0.017 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|small-subunit processome assembly|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATPase activator activity|RNA binding nucleolus|90S preribosome|cytoplasm YAL034W-A YAL034W-A MTW1 het Verified 1.72156426545594 0.0425742405747338 no 79 0.0278514786754656 141 0.002 FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly chromosome segregation|protein localization to kinetochore molecular_function nuclear MIS12/MIND type complex|spindle pole YPL043W YPL043W NOP4 het Verified 1.7195196855068 0.0427598931601965 no 80 0.0297523539884012 159 0.009 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) rRNA processing RNA binding nucleolus YBR257W YBR257W POP4 het Verified 1.71063007219136 0.0435747119709771 no 81 0.0519731233189109 226 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YOR224C YOR224C RPB8 het Verified 1.70928216877825 0.0436993473220211 no 82 0.117488617116961 435 0.015 FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YIL046W YIL046W MET30 het Verified 1.6947724933067 0.0450592937248708 no 83 0.0450324245832325 229 0.000 FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus response to cadmium ion|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|regulation of DNA-dependent DNA replication initiation|M phase of mitotic cell cycle|response to arsenic-containing substance|G1/S transition of mitotic cell cycle|protein polyubiquitination|regulation of transcription involved in G1 phase of mitotic cell cycle|protein ubiquitination ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus YOR372C YOR372C NDD1 het Verified 1.69449451640263 0.0450856764157413 no 84 0.0503305571208862 268 0.007 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE NUCLEUS Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes positive regulation of transcription involved in G2/M-phase of mitotic cell cycle transcription coactivator activity nucleus YNL258C YNL258C DSL1 het Verified 1.6875018909126 0.0457534433181283 no 85 0.0503318691348216 258 0.010 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene retrograde vesicle-mediated transport, Golgi to ER|ER-dependent peroxisome organization molecular_function Dsl1p complex|endoplasmic reticulum|peroxisome|nucleus YPR168W YPR168W NUT2 het Verified 1.67723075334707 0.0467486837560612 no 86 0.0322912151504563 188 0.008 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YPR165W YPR165W RHO1 het Verified 1.66945493104282 0.0475136275521931 no 87 0.11552115192594 546 0.016 FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|CYTOSKELETON ORGANIZATION MITOCHONDRION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) regulation of fungal-type cell wall (1->3)-alpha-glucan biosynthetic process|regulation of fungal-type cell wall organization|positive regulation of endocytosis|budding cell bud growth|small GTPase mediated signal transduction|regulation of protein localization|positive regulation of protein kinase C signaling cascade|actin cytoskeleton organization|regulation of vacuole fusion, non-autophagic|actin cytoskeleton reorganization|regulation of exocyst localization|regulation of cell size GTPase activity|signal transducer activity incipient cellular bud site|mitochondrion|Golgi apparatus|peroxisome|cellular bud neck|cellular bud tip|mitochondrial outer membrane|1,3-beta-D-glucan synthase complex|plasma membrane|mating projection tip YOR335C YOR335C ALA1 het Verified 1.65337926215374 0.0491268503506768 no 88 0.0288116997340615 170 0.011 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog alanyl-tRNA aminoacylation|mitochondrial alanyl-tRNA aminoacylation alanine-tRNA ligase activity mitochondrion|cytoplasm YOR210W YOR210W RPB10 het Verified 1.64287867172303 0.0502040193399279 no 89 0.0893173100955012 420 0.012 FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase II, core complex YIL068C YIL068C SEC6 het Verified 1.6310555614682 0.0514392981374848 no 90 0.0508120974947709 237 0.025 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p exocyst localization|Golgi to plasma membrane transport|exocytosis|vesicle fusion|negative regulation of SNARE complex assembly|vesicle docking involved in exocytosis protein homodimerization activity mating projection tip|cellular bud neck|cellular bud tip|exocyst YER104W YER104W RTT105 het Verified 1.60646948480644 0.0540853972354666 no 91 0.0655269324623514 278 0.009 FT NUCLEUS Protein with a role in regulation of Ty1 transposition negative regulation of transposition, RNA-mediated molecular_function nucleus|cytoplasm YIL004C YIL004C BET1 het Verified 1.59728073284464 0.0551015718175 no 92 0.0632411481688392 317 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum membrane|ER to Golgi transport vesicle YCL059C YCL059C KRR1 het Verified 1.59674282917524 0.055161522224001 no 93 0.0367519341068464 196 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit biogenesis|rRNA processing molecular_function small-subunit processome|nucleolus|90S preribosome YPR110C YPR110C RPC40 het Verified 1.59417546436503 0.0554483701592215 no 94 0.0768767209418288 271 0.004 FT NUCLEUS RNA polymerase subunit AC40, common to RNA polymerase I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex YBR049C YBR049C REB1 het Verified 1.58987340964947 0.0559316712203925 no 95 0.061377880961203 279 0.001 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication termination of RNA polymerase I transcription|regulation of transcription from RNA polymerase II promoter sequence-specific DNA binding nucleoplasm YKL108W YKL108W SLD2 het Verified 1.58916172856135 0.0560119422154848 no 96 0.0269094876689824 156 0.000 FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress DNA replication preinitiation complex assembly|DNA strand renaturation|DNA replication initiation|double-strand break repair via break-induced replication|mitotic cell cycle DNA replication checkpoint DNA replication origin binding|single-stranded DNA binding DNA replication preinitiation complex YOR340C YOR340C RPA43 het Verified 1.58905461851185 0.056024031093769 no 97 0.141652873079465 514 0.009 FF|FT NUCLEUS RNA polymerase I subunit A43 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex YGL130W YGL130W CEG1 het Verified 1.57984838256461 0.0570707962414615 no 98 0.0374214771819509 215 0.003 FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 7-methylguanosine mRNA capping|positive regulation of transcription from RNA polymerase II promoter mRNA guanylyltransferase activity mRNA cap methyltransferase complex|nucleus YJL025W YJL025W RRN7 het Verified 1.57641519104698 0.0574650748586827 no 99 0.0521269602090041 274 0.001 FT NUCLEUS Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I CORE element sequence-specific DNA binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity nucleolus|RNA polymerase I core factor complex YGL075C YGL075C MPS2 het Verified 1.57527935616671 0.0575959884462308 no 100 0.0677389819037857 333 0.026 FT CELL CYCLE|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p mitotic anaphase|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton nuclear envelope|spindle pole body YKR037C YKR037C SPC34 het Verified 1.54961005642862 0.0606175688843199 no 101 0.0596351514398048 265 0.010 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|DASH complex|condensed nuclear chromosome kinetochore|spindle pole body|spindle YLR275W YLR275W SMD2 het Verified 1.53930315851929 0.0618651516149611 no 102 0.0930680619220026 396 0.004 FT RNA PROCESSING NUCLEUS Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 mRNA splicing, via spliceosome molecular_function U1 snRNP|U4/U6 x U5 tri-snRNP complex|U2-type prespliceosome|U5 snRNP YNL114C_d YNL114C YNL114C het Dubious 1.53574347415683 0.0623006540425275 no 103 0.0946718901838776 410 0.003 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Unknown Unknown Unknown YMR061W YMR061W RNA14 het Verified 1.53312286453358 0.0626227925270049 no 104 0.0753591962318467 301 0.006 FT RNA PROCESSING NUCLEUS|MITOCHONDRION Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation RNA binding|protein heterodimerization activity mRNA cleavage factor complex|mitochondrion|nucleus YAL003W YAL003W EFB1 het Verified 1.52720541946527 0.0633549695589181 no 105 0.0468138459432612 224 0.018 FT TRANSLATION RIBOSOME Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site translational elongation translation elongation factor activity|guanyl-nucleotide exchange factor activity ribosome|eukaryotic translation elongation factor 1 complex YIL061C YIL061C SNP1 het Verified 1.50951328066859 0.0655838319994662 no 106 0.0750804970260846 332 0.005 FT RNA PROCESSING NUCLEUS Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding|U1 snRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YDR054C YDR054C CDC34 het Verified 1.50439012674733 0.0662404724877051 no 107 0.040059045168501 229 0.015 FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein autoubiquitination|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle|protein polyubiquitination ubiquitin-protein ligase activity|protein homodimerization activity SCF ubiquitin ligase complex|nucleus|cytoplasm YLL008W YLL008W DRS1 het Verified 1.4890964283414 0.06823098909923 no 108 0.0675880205448539 325 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles ribosomal large subunit assembly|rRNA processing ATP-dependent RNA helicase activity nucleolus|preribosome, large subunit precursor YIL142W YIL142W CCT2 het Verified 1.48166713779141 0.0692144426114571 no 109 0.0882305395294187 408 0.003 Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YKL125W YKL125W RRN3 het Verified 1.47896941154616 0.0695742451211402 no 110 0.0817777184079159 316 0.006 FT NUCLEUS Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo transcription initiation from RNA polymerase I promoter for nuclear large rRNA transcript core RNA polymerase I binding transcription factor activity|RNA polymerase I transcription factor binding transcription factor activity|RNA polymerase I transcription factor binding|RNA polymerase I core binding nucleolus YJL035C YJL035C TAD2 het Verified 1.47346672936992 0.0703126143278022 no 111 0.0437475607733686 220 0.007 FT RNA PROCESSING NUCLEUS Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YOL021C YOL021C DIS3 het Verified 1.46624026952362 0.071291420741695 no 112 0.123775089133508 425 0.009 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress nuclear mRNA surveillance|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nonfunctional rRNA decay|nuclear polyadenylation-dependent mRNA catabolic process|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ncRNA 3'-end processing 3'-5'-exoribonuclease activity|endoribonuclease activity|tRNA binding mitochondrion|nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YBR192W YBR192W RIM2 het Verified 1.45696150152473 0.0725635037843736 no 113 0.0914876917998949 460 0.005 FF|FT ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family pyrimidine nucleotide transport|mitochondrial genome maintenance|transmembrane transport pyrimidine nucleotide transmembrane transporter activity|transporter activity mitochondrion|integral to membrane YMR047C YMR047C NUP116 het Verified 1.45160932841538 0.0733051317397304 no 114 0.0544890248218214 252 0.006 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p) poly(A)+ mRNA export from nucleus|mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YHR005C-A YHR005C-A TIM10 het Verified 1.44598028796378 0.0740913653728093 no 115 0.0742319255636927 323 0.001 FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane protein import into mitochondrial inner membrane protein transporter activity|unfolded protein binding mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex|mitochondrial intermembrane space protein transporter complex YHR148W YHR148W IMP3 het Verified 1.44474007308245 0.0742654546832371 no 116 0.103611036948618 346 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA rRNA processing|ribosome biogenesis snoRNA binding small-subunit processome|Mpp10 complex|90S preribosome YHR172W YHR172W SPC97 het Verified 1.44075577581435 0.0748268456800759 no 117 0.0872290818272781 366 0.004 FT CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque microtubule nucleation structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YGR120C YGR120C COG2 het Verified 1.43724442179724 0.0753242774377129 no 118 0.0923772503360537 377 0.014 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|ER to Golgi vesicle-mediated transport|macroautophagy|intra-Golgi vesicle-mediated transport|retrograde transport, vesicle recycling within Golgi|CVT pathway molecular_function Golgi transport complex|extrinsic to membrane YGL123W YGL123W RPS2 het Verified 1.42276181978191 0.077402606434252 no 119 0.131785818525065 498 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION NUCLEUS RIBOSOME Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 positive regulation of translational fidelity|rRNA export from nucleus structural constituent of ribosome|SSU rRNA binding small-subunit processome|cytosolic small ribosomal subunit YKL060C YKL060C FBA1 het Verified 1.42258246584147 0.0774286149660333 no 120 0.0569673595685029 268 0.004 FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress gluconeogenesis|glycolysis fructose-bisphosphate aldolase activity cytosol|mitochondrion|cytoplasm YGR158C YGR158C MTR3 het Verified 1.4190129085166 0.0779476263628517 no 121 0.0885821892212133 373 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) nuclear mRNA surveillance|nuclear polyadenylation-dependent CUT catabolic process|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay molecular_function nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YIL026C YIL026C IRR1 het Verified 1.41666952959928 0.0782897848304505 no 122 0.108548915795528 455 0.002 FF|FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability spore germination|fungal-type cell wall organization|cytogamy|mitotic sister chromatid cohesion chromatin binding nucleus|nuclear mitotic cohesin complex YOR282W_d YOR282W YOR282W het Dubious 1.41450007553369 0.0786075626091065 no 123 0.0689287395378405 304 0.001 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Unknown Unknown Unknown YMR013C YMR013C SEC59 het Verified 1.41335286710204 0.0787759983484244 no 124 0.0622233828432165 278 0.006 FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation protein glycosylation dolichol kinase activity integral to membrane|endoplasmic reticulum membrane YJL015C_d YJL015C YJL015C het Dubious 1.4068612785542 0.0797342637456102 no 125 0.0731470120705318 352 0.001 Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Unknown Unknown Unknown YBL034C YBL034C STU1 het Verified 1.40626185150624 0.0798231918335335 no 126 0.0510595755542827 228 0.003 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME|CYTOSKELETON Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles microtubule nucleation structural constituent of cytoskeleton spindle pole body YML093W YML093W UTP14 het Verified 1.40310125349314 0.0802933237239276 no 127 0.0884173556856101 336 0.010 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YJR017C YJR017C ESS1 het Verified 1.39308091655987 0.0817976615833177 no 128 0.0683864440510677 270 0.013 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEOLYSIS|GENE SILENCING NUCLEUS Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 termination of RNA polymerase II transcription|positive regulation of protein dephosphorylation|negative regulation of histone deacetylation|negative regulation of transcription from RNA polymerase II promoter|protein peptidyl-prolyl isomerization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|Unknown|regulation of transcription involved in G1 phase of mitotic cell cycle|positive regulation of transcription from RNA polymerase II promoter|regulation of transcription involved in G2/M-phase of mitotic cell cycle peptidyl-prolyl cis-trans isomerase activity|RNA polymerase II core binding nucleus YPR016C YPR016C TIF6 het Verified 1.37959525101754 0.0838556502308231 no 129 0.134482444136667 512 0.009 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits ribosomal subunit export from nucleus|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing ribosomal large subunit binding nucleolus|nucleus|preribosome, large subunit precursor|cytoplasm YOR261C YOR261C RPN8 het Verified 1.37049831226623 0.0852657009408462 no 130 0.0664201563809926 297 0.003 FT PROTEOLYSIS Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p ubiquitin-dependent protein catabolic process molecular_function proteasome storage granule|proteasome regulatory particle, lid subcomplex YOR048C YOR048C RAT1 het Verified 1.36781799861681 0.0856845262045928 no 131 0.0973503879597021 400 0.013 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination termination of RNA polymerase II transcription, exosome-dependent|nuclear mRNA surveillance|termination of RNA polymerase II transcription, poly(A)-coupled|rRNA processing|RNA processing 5'-3' exoribonuclease activity mitochondrion|nucleus YDL004W YDL004W ATP16 het Verified 1.35467279426862 0.087760914726733 no 132 0.0837234192513287 374 0.002 FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS MITOCHONDRION Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated ATP synthesis coupled proton transport hydrogen ion transporting ATP synthase activity, rotational mechanism|ATPase activity|proton-transporting ATPase activity, rotational mechanism proton-transporting ATP synthase complex, catalytic core F(1)|mitochondrion|integral to membrane|mitochondrial proton-transporting ATP synthase, central stalk YDR412W YDR412W RRP17 het Verified 1.35233653664982 0.0881338406624551 no 133 0.049992172276223 241 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA rRNA processing molecular_function cytoplasm YNL221C YNL221C POP1 het Verified 1.35062661700815 0.08840753501893 no 134 0.088465099630867 409 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YNL172W YNL172W APC1 het Verified 1.34369037686974 0.0895242652213238 no 135 0.058800727778097 263 0.002 FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS|CYTOSKELETON UBIQUITIN LIGASE COMPLEX Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress mitotic metaphase/anaphase transition|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|exit from mitosis|cyclin catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YNL038W YNL038W GPI15 het Verified 1.3419377553066 0.0898080893393545 no 136 0.0752629189201531 326 0.029 FT LIPID METABOLISM ENDOPLASMIC RETICULUM Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YDR407C YDR407C TRS120 het Verified 1.31340429119023 0.0945233761495723 no 137 0.0952327892314065 396 0.000 FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic regulation of Rab GTPase activity|early endosome to Golgi transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity trans-Golgi network|TRAPP complex|early endosome YJL069C YJL069C UTP18 het Verified 1.313281995869 0.0945439714959518 no 138 0.0691911312898671 296 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YDR527W YDR527W RBA50 het Verified 1.30661110525169 0.0956724115417288 no 139 0.101924835756196 424 0.018 FF|FT TRANSCRIPTION FROM RNA POL II Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 transcription from RNA polymerase II promoter molecular_function cytoplasm YDR327W_d YDR327W YDR327W het Dubious 1.28889281032899 0.0987176769553446 no 140 0.0804439776774346 285 0.002 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Unknown Unknown Unknown YNR043W YNR043W MVD1 het Verified 1.28683106526677 0.0990765883041716 no 141 0.105605227844606 405 0.004 FT LIPID METABOLISM Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer sterol biosynthetic process diphosphomevalonate decarboxylase activity cytoplasm YBR004C YBR004C GPI18 het Verified 1.28254697502414 0.0998254187032034 no 142 0.102668135154274 487 0.002 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria GPI anchor biosynthetic process mannosyltransferase activity|dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to endoplasmic reticulum membrane|mitochondrion|mannosyltransferase complex|integral to membrane|endoplasmic reticulum YPL235W YPL235W RVB2 het Verified 1.28060079656336 0.100166960041481 no 143 0.167276272482957 586 0.002 FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly regulation of transcription from RNA polymerase II promoter|rRNA processing|chromatin remodeling|box C/D snoRNP assembly|histone exchange ATP-dependent 5'-3' DNA helicase activity|ATP-dependent 3'-5' DNA helicase activity nuclear telomeric heterochromatin|ASTRA complex|R2TP complex|Swr1 complex|nucleus|Ino80 complex YBR140C YBR140C IRA1 het Verified 1.278967819188 0.100454194220709 no 144 0.188501631406086 667 0.000 FF|FT SIGNALING|NUCLEOTIDE METABOLISM MITOCHONDRION|PLASMA MEMBRANE GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication negative regulation of cAMP biosynthetic process|negative regulation of Ras protein signal transduction|regulation of adenylate cyclase activity|positive regulation of Ras GTPase activity Ras GTPase activator activity mitochondrion|integral to membrane|membrane YLR458W_d YLR458W YLR458W het Dubious 1.27849186334238 0.100538025949242 no 145 0.0918266296215699 361 0.001 Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Unknown Unknown Unknown YMR309C YMR309C NIP1 het Verified 1.27791308335908 0.100640037199643 no 146 0.119156504438074 442 0.013 FT TRANSLATION eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YPR182W YPR182W SMX3 het Verified 1.26503905814764 0.102928663475288 no 147 0.0763184204670651 316 0.003 FT RNA PROCESSING NUCLEUS Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F mRNA splicing, via spliceosome molecular_function U1 snRNP|U4/U6 x U5 tri-snRNP complex|U5 snRNP YPL211W YPL211W NIP7 het Verified 1.26235157588139 0.10341115279881 no 148 0.0986392094696803 375 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RIBOSOME Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p ribosomal large subunit assembly|ribosomal large subunit biogenesis|rRNA processing molecular_function cytosolic large ribosomal subunit|nucleolus|preribosome, large subunit precursor YDR328C YDR328C SKP1 het Verified 1.2594362960281 0.103936393174123 no 149 0.0833792846756972 331 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|ION HOMEOSTASIS|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|ION HOMEOSTASIS NUCLEUS|CHROMOSOME UBIQUITIN LIGASE COMPLEX|KINETOCHORE Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress kinetochore assembly|protein complex assembly|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|regulation of protein complex assembly|G1/S transition of mitotic cell cycle|septin ring assembly|regulation of exit from mitosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein neddylation|vacuolar acidification|cytokinesis ubiquitin-protein ligase activity|DNA replication origin binding|protein binding RAVE complex|SCF ubiquitin ligase complex|nucleus|CBF3 complex|kinetochore|cytoplasm YDL166C YDL166C FAP7 het Verified 1.25845753548134 0.104113167662768 no 150 0.117935415830573 399 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO OXIDATIVE STRESS NUCLEUS Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D cellular response to oxidative stress|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit export from nucleus nucleoside-triphosphatase activity nucleus|preribosome|cytoplasm YAL041W YAL041W CDC24 het Verified 1.25139486794424 0.105395224373824 no 151 0.121495522302849 469 0.003 FT CELL CYCLE|SIGNALING NUCLEUS|SITE OF POLARIZED GROWTH Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of exit from mitosis|establishment of cell polarity|small GTPase mediated signal transduction Rho guanyl-nucleotide exchange factor activity incipient cellular bud site|nucleus|cellular bud neck|mating projection tip|cellular bud tip|cytoplasm YDL193W YDL193W NUS1 het Verified 1.24847378486501 0.105928801432715 no 152 0.173036442254375 595 0.001 FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY protein glycosylation prenyltransferase activity nuclear envelope|lipid particle|endoplasmic reticulum YHR036W YHR036W BRL1 het Verified 1.24757304447974 0.106093727507072 no 153 0.151991824233398 560 0.001 FF|FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export mRNA export from nucleus|protein export from nucleus|nuclear envelope organization molecular_function nuclear envelope|endoplasmic reticulum YGR005C YGR005C TFG2 het Verified 1.24636100613291 0.106315945072206 no 154 0.0837179149781596 366 0.001 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 transcriptional start site selection at RNA polymerase II promoter|transcription initiation from RNA polymerase II promoter|promoter clearance from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|core RNA polymerase II recruiting transcription factor activity transcription factor TFIIF complex YGL201C YGL201C MCM6 het Verified 1.24582895312535 0.106413598840544 no 155 0.110752644126819 398 0.001 FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|double-strand break repair via break-induced replication|pre-replicative complex assembly|DNA replication initiation four-way junction helicase activity|DNA helicase activity|DNA replication origin binding|single-stranded DNA-dependent ATPase activity|ATP-dependent 3'-5' DNA helicase activity replication fork protection complex|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YHR083W YHR083W SAM35 het Verified 1.24524383745203 0.106521066566466 no 156 0.126373278188819 451 0.001 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane molecular_function mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion YDL221W_d YDL221W YDL221W het Dubious 1.24434862029151 0.106685641993109 no 157 0.0945023585396347 378 0.008 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13 Unknown Unknown Unknown YBR124W_d YBR124W YBR124W het Dubious 1.23662853953691 0.108112509762438 no 158 0.0888581541646363 376 0.000 Putative protein of unknown function Unknown Unknown Unknown YDR437W YDR437W GPI19 het Verified 1.23653170714398 0.108130493679148 no 159 0.103037253790631 360 0.006 FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|nuclear envelope|integral to endoplasmic reticulum membrane|endoplasmic reticulum YLR274W YLR274W MCM5 het Verified 1.23076644648657 0.109205114133676 no 160 0.134204862512255 466 0.003 FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase S phase of mitotic cell cycle|establishment of chromatin silencing|DNA replication initiation|regulation of DNA-dependent DNA replication initiation|negative regulation of chromatin silencing at telomere|pre-replicative complex assembly|double-strand break repair via break-induced replication|chromatin silencing at telomere DNA helicase activity|chromatin binding|DNA replication origin binding|single-stranded DNA binding replication fork protection complex|nuclear chromosome, telomeric region|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YKL052C YKL052C ASK1 het Verified 1.22762432311484 0.109794013381893 no 161 0.0661741206465794 256 0.006 FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress attachment of spindle microtubules to kinetochore|attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization|mitotic sister chromatid segregation microtubule binding|structural constituent of cytoskeleton condensed nuclear chromosome kinetochore|DASH complex|condensed nuclear chromosome, centromeric region|mitotic spindle YPL011C YPL011C TAF3 het Verified 1.22182277062425 0.110887327804657 no 162 0.082942934670969 313 0.006 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly transcription factor TFIID complex YNL207W YNL207W RIO2 het Verified 1.21591582455655 0.112008494626902 no 163 0.127360773933173 461 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|PROTEIN PHOSPHORYLATION NUCLEUS Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) nucleocytoplasmic transporter activity|protein kinase activity cytosol|nucleus|cytoplasm YHR143W-A YHR143W-A RPC10 het Verified 1.21471924116626 0.112236595679181 no 164 0.133964114176692 474 0.002 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YPR142C_d YPR142C YPR142C het Dubious 1.21271345587416 0.112619696727729 no 165 0.0912533193095946 344 0.003 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Unknown Unknown Unknown YKL095W YKL095W YJU2 het Verified 1.20950876544619 0.113233722793544 no 166 0.104361094296457 398 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing generation of catalytic spliceosome for first transesterification step first spliceosomal transesterification activity nucleus|U2-type catalytic step 1 spliceosome YPL217C YPL217C BMS1 het Verified 1.20797510610946 0.113528418953837 no 167 0.143091922345259 511 0.016 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM NUCLEUS|MITOCHONDRION GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p ribosome assembly|rRNA processing U3 snoRNA binding|GTP binding|GTPase activity mitochondrion|nucleolus|nucleus|90S preribosome|cytoplasm YER147C YER147C SCC4 het Verified 1.19148985601049 0.116730668276997 no 168 0.141747333316483 442 0.003 FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX double-strand break repair|establishment of protein localization to chromatin|rDNA condensation|mitotic chromosome condensation|mitotic sister chromatid cohesion molecular_function nuclear chromatin|SMC loading complex YGR099W YGR099W TEL2 het Verified 1.18826253796412 0.117364995984862 no 169 0.151506460859593 492 0.013 FT PROTEIN LOCALIZATION NUCLEUS|CHROMOSOME Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 protein localization to chromosome|telomere maintenance via telomerase|telomere maintenance telomeric DNA binding ASTRA complex|nuclear telomere cap complex YMR001C YMR001C CDC5 het Verified 1.17557522745702 0.119882329790014 no 170 0.110261671553001 430 0.003 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate synaptonemal complex disassembly|spindle assembly involved in meiosis|protein phosphorylation|resolution of meiotic recombination intermediates|positive regulation of spindle pole body separation protein kinase activity nucleus|cellular bud neck|spindle pole YDL145C YDL145C COP1 het Verified 1.17266666306772 0.120464752508556 no 171 0.111088664008522 426 0.011 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YPR055W YPR055W SEC8 het Verified 1.16176553583226 0.122665358448561 no 172 0.128717041878786 429 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Golgi inheritance|endoplasmic reticulum inheritance|Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|cellular bud neck|mating projection tip|exocyst|cellular bud tip YML049C YML049C RSE1 het Verified 1.16059159589832 0.122904012373889 no 173 0.126303023052003 470 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING NUCLEUS Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport spliceosomal complex assembly|mRNA splicing, via spliceosome U2 snRNA binding U2 snRNP|U2-type prespliceosome YEL002C YEL002C WBP1 het Verified 1.15596950392884 0.123846815553492 no 174 0.129453799943856 417 0.002 FT CELL CYCLE|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum cell cycle|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity nuclear envelope|oligosaccharyltransferase complex|endoplasmic reticulum YJL061W YJL061W NUP82 het Verified 1.15374265250669 0.124302845830148 no 175 0.114898296897043 439 0.003 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) mRNA export from nucleus|protein export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|ribosomal small subunit export from nucleus structural constituent of nuclear pore integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore linkers|nuclear pore YNL282W YNL282W POP3 het Verified 1.15074429327595 0.124918724250238 no 176 0.114441522908859 458 0.008 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YOR236W YOR236W DFR1 het Verified 1.14261742934001 0.126598732903013 no 177 0.129952796184567 539 0.009 FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism tetrahydrofolate biosynthetic process|dihydrofolate metabolic process dihydrofolate reductase activity|mRNA binding cytosol YDR141C YDR141C DOP1 het Verified 1.13327999585146 0.128548344807323 no 178 0.137172790325223 504 0.002 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|MITOCHONDRION|GOLGI APPARATUS Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies endoplasmic reticulum organization|cell morphogenesis|Golgi to endosome transport molecular_function trans-Golgi network|endosome|mitochondrion|cytoplasm YCR057C YCR057C PWP2 het Verified 1.13101787657554 0.129023783673167 no 179 0.162643221856537 531 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION NUCLEUS Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|establishment of cell polarity|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cytokinesis snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|cytoplasm|90S preribosome YPL231W YPL231W FAS2 het Verified 1.129808322922 0.129278500204463 no 180 0.114122377499628 400 0.003 FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities fatty acid biosynthetic process holo-[acyl-carrier-protein] synthase activity|3-oxoacyl-[acyl-carrier-protein] synthase activity|fatty acid synthase activity|3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity cytosol|fatty acid synthase complex|mitochondrion|cytoplasm YHR196W YHR196W UTP9 het Verified 1.12715185810943 0.129839140429598 no 181 0.154200388321953 497 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|nucleus|90S preribosome YNL262W YNL262W POL2 het Verified 1.12394016159258 0.130519206025294 no 182 0.0913963662300019 398 0.006 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING NUCLEUS|CHROMOSOME Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p DNA replication involved in S phase|double-strand break repair via nonhomologous end joining|DNA synthesis involved in DNA repair|DNA-dependent DNA replication|mitotic cell cycle DNA replication checkpoint|mitotic sister chromatid cohesion|chromatin silencing at telomere double-stranded DNA binding|single-stranded DNA binding|DNA-directed DNA polymerase activity replication fork|epsilon DNA polymerase complex YFL039C YFL039C ACT1 het Verified 1.11843035777167 0.131691617382587 no 183 0.255591949441834 859 0.003 FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|CELL CYCLE|MEMBRANE ORGANIZATION|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|RESPON NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON HISTONE ACETYLTRANSFERASE Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions ascospore wall assembly|vacuole inheritance|protein secretion|DNA repair|budding cell isotropic bud growth|mitochondrion inheritance|fungal-type cell wall organization|vesicle transport along actin filament|establishment of cell polarity|chronological cell aging|cytokinesis|actin filament reorganization involved in cell cycle|histone acetylation|endocytosis|exocytosis|establishment of mitotic spindle orientation|regulation of transcription from RNA polymerase II promoter|actomyosin contractile ring contraction|cellular response to oxidative stress structural constituent of cytoskeleton cellular bud neck contractile ring|actin filament|histone acetyltransferase complex|Swr1 complex|actin filament bundle|NuA4 histone acetyltransferase complex|Ino80 complex|actin cortical patch YNL222W YNL222W SSU72 het Verified 1.11543203138678 0.132332664815881 no 184 0.0883443444861233 364 0.001 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo transcription initiation from RNA polymerase II promoter|mRNA 3'-end processing|mRNA cleavage|termination of RNA polymerase II transcription|termination of RNA polymerase II transcription, poly(A)-coupled|transcription antitermination|transcriptional start site selection at RNA polymerase II promoter|dephosphorylation of RNA polymerase II C-terminal domain|termination of RNA polymerase II transcription, exosome-dependent|snoRNA transcription|transcription elongation from RNA polymerase II promoter protein tyrosine phosphatase activity|phosphoprotein phosphatase activity|CTD phosphatase activity mRNA cleavage and polyadenylation specificity factor complex|nucleus YPL243W YPL243W SRP68 het Verified 1.11433991740036 0.132566694191648 no 185 0.112644363653127 376 0.002 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS NUCLEUS Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding signal recognition particle, endoplasmic reticulum targeting YER012W YER012W PRE1 het Verified 1.11208159479427 0.133051534720748 no 186 0.171714595298517 604 0.004 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activator activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YNL002C YNL002C RLP7 het Verified 1.10857730191344 0.133806287217975 no 187 0.140566594853965 495 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION NUCLEUS Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA binding nucleolus|preribosome, large subunit precursor YOL127W YOL127W RPL25 het Verified 1.10449143845019 0.134690007708785 no 188 0.177983643552031 642 0.002 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YDL235C YDL235C YPD1 het Verified 1.102222662796 0.135182440865217 no 189 0.143543102513542 438 0.007 FT SIGNALING NUCLEUS Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus osmosensory signaling pathway via two-component system protein histidine kinase binding|transferase activity, transferring phosphorus-containing groups nucleus|cytoplasm YDL143W YDL143W CCT4 het Verified 1.09517559697122 0.136719857259517 no 190 0.216226143330742 725 0.009 FF Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YCR035C YCR035C RRP43 het Verified 1.08576810764893 0.138790799810595 no 191 0.180230661390858 647 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nucleoplasm|nucleolus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex)|cytoplasm YLR029C YLR029C RPL15A het Verified 1.08204801869828 0.139615595809219 no 192 0.189822948732083 646 0.003 FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YNL113W YNL113W RPC19 het Verified 1.07927062998529 0.140233550838386 no 193 0.163145941054484 602 0.003 FT NUCLEUS RNA polymerase subunit AC19, common to RNA polymerases I and III tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase III complex YDR064W YDR064W RPS13 het Verified 1.07614564535885 0.14093106217035 no 194 0.165642979935369 609 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome|SSU rRNA binding cytosolic small ribosomal subunit|90S preribosome YFL029C YFL029C CAK1 het Verified 1.07389190941319 0.141435564951465 no 195 0.172697409576147 642 0.001 FT CELL CYCLE|PROTEIN PHOSPHORYLATION Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases G2/M transition of mitotic cell cycle|meiosis|G1 phase of mitotic cell cycle|protein phosphorylation cyclin-dependent protein kinase activating kinase activity cytoplasm YDR390C YDR390C UBA2 het Verified 1.07315852448419 0.141599998081042 no 196 0.133478586717818 453 0.000 FT NUCLEUS Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity SUMO activating enzyme complex|nucleus YFR050C YFR050C PRE4 het Verified 1.07301318966938 0.14163259914703 no 197 0.167456330707058 608 0.016 FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 7 subunit of the 20S proteasome proteasome assembly|proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YNL088W YNL088W TOP2 het Verified 1.05671453006226 0.145320945817604 no 198 0.135785566640205 486 0.000 FT CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CHROMOSOME Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation chromatin assembly or disassembly|chromatin remodeling at centromere|replication fork progression beyond termination site|mitotic cell cycle G2/M transition decatenation checkpoint|DNA topological change|DNA strand elongation involved in DNA replication|regulation of mitotic recombination|reciprocal meiotic recombination DNA topoisomerase (ATP-hydrolyzing) activity DNA replication termination region|synaptonemal complex|mitochondrion|nucleus YPL146C YPL146C NOP53 het Verified 1.05653917632252 0.145360976115594 no 199 0.131763281296017 478 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired ribosomal large subunit export from nucleus|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA binding nucleolus|nucleus YGL093W YGL093W SPC105 het Verified 1.05333944849559 0.146092722562252 no 200 0.205209961766036 792 0.002 FF|FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION|CHROMOSOME|CYTOSKELETON KINETOCHORE Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components microtubule nucleation|protein localization to kinetochore|sister chromatid biorientation|mitotic cell cycle spindle assembly checkpoint microtubule binding|structural constituent of cytoskeleton mitochondrion|condensed nuclear chromosome kinetochore|spindle pole body YMR128W YMR128W ECM16 het Verified 1.05158523033804 0.146494943500396 no 201 0.135089503579098 510 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosome biogenesis RNA helicase activity small-subunit processome|mitochondrion|nucleolus|90S preribosome YNL312W YNL312W RFA2 het Verified 1.04884433830968 0.147124883550701 no 202 0.106646387781414 383 0.003 FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YDR472W YDR472W TRS31 het Verified 1.04538779160858 0.147921888424897 no 203 0.172613241630479 550 0.005 FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM|GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YJR006W YJR006W POL31 het Verified 1.04062652392146 0.149024458115559 no 204 0.131645489758132 474 0.002 FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair mismatch repair|RNA-dependent DNA replication|lagging strand elongation|base-excision repair|postreplication repair|DNA replication, removal of RNA primer|leading strand elongation|nucleotide-excision repair single-stranded DNA specific 3'-5' exodeoxyribonuclease activity|DNA-directed DNA polymerase activity delta DNA polymerase complex YGR094W YGR094W VAS1 het Verified 1.03887161836156 0.14943222381124 no 205 0.153023028382738 530 0.009 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Mitochondrial and cytoplasmic valyl-tRNA synthetase valyl-tRNA aminoacylation valine-tRNA ligase activity mitochondrion|cytoplasm YLR147C YLR147C SMD3 het Verified 1.03019224579647 0.151459884521292 no 206 0.148499959470087 567 0.009 FT RNA PROCESSING NUCLEUS Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YNL290W YNL290W RFC3 het Verified 1.02163889950479 0.153475919763388 no 207 0.132353821461704 457 0.012 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity|ATPase activity Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|nucleus|Ctf18 RFC-like complex YPL122C YPL122C TFB2 het Verified 1.00914363202283 0.15645287762846 no 208 0.163498318907318 596 0.001 FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter double-stranded DNA binding|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YPR085C YPR085C ASA1 het Verified 1.00843924922606 0.156621819269435 no 209 0.193363302844092 648 0.012 FT CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 biological_process molecular_function ASTRA complex YPR086W YPR086W SUA7 het Verified 0.996539925929527 0.159493939008664 no 210 0.170663675535888 564 0.001 FT TRANSCRIPTION FROM RNA POL II|TRANSLATION NUCLEUS Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II transcriptional start site selection at RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|Unknown TBP-class protein binding|RNA polymerase II core binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|core RNA polymerase II recruiting transcription factor activity nucleus YOR174W YOR174W MED4 het Verified 0.996028819897565 0.159618071225019 no 211 0.201615042712273 700 0.003 FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation transcription from RNA polymerase II promoter molecular_function core mediator complex YBR109C YBR109C CMD1 het Verified 0.992691330939573 0.160430200453617 no 212 0.178731029236385 615 0.003 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|LIPID METABOLISM|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin karyogamy involved in conjugation with cellular fusion|phosphatidylinositol biosynthetic process|NLS-bearing substrate import into nucleus|microautophagy|cell budding|vacuole fusion, non-autophagic|receptor-mediated endocytosis|cytoskeleton organization|spindle pole body organization|transcription factor import into nucleus calcium-dependent protein binding|protein binding|calcium ion binding incipient cellular bud site|central plaque of spindle pole body|mating projection tip|cellular bud neck|cellular bud tip YFL002C YFL002C SPB4 het Verified 0.989942960542981 0.161100999797333 no 213 0.19102462107758 696 0.001 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients ribosomal large subunit assembly|rRNA processing ATP-dependent RNA helicase activity nucleolus|90S preribosome YDR016C YDR016C DAD1 het Verified 0.988968268478758 0.161339333510795 no 214 0.169446358196808 580 0.002 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton condensed nuclear chromosome kinetochore|DASH complex|spindle YOR362C YOR362C PRE10 het Verified 0.986871784553679 0.161852748966238 no 215 0.211194980148156 738 0.000 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process mRNA binding mitochondrion|proteasome core complex, alpha-subunit complex|nucleus|proteasome storage granule|nuclear outer membrane-endoplasmic reticulum membrane network YML015C YML015C TAF11 het Verified 0.986833609673269 0.161862107607001 no 216 0.171792147549044 599 0.014 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly transcription factor TFIID complex YNL137C YNL137C NAM9 het Verified 0.973149658285942 0.165239463323529 no 217 0.168900525426939 600 0.005 FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal component of the small subunit mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YER025W YER025W GCD11 het Verified 0.968453691989408 0.166408917791215 no 218 0.181581805361232 647 0.003 FT TRANSLATION|NUCLEOTIDE METABOLISM RIBOSOME Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met positive regulation of translational fidelity GTP binding|tRNA binding|translation initiation factor activity multi-eIF complex|ribosome|eukaryotic translation initiation factor 2 complex YMR197C YMR197C VTI1 het Verified 0.965671295945893 0.167104343531458 no 219 0.218069721050521 741 0.003 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Golgi to vacuole transport|vacuole fusion, non-autophagic|intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|integral to Golgi membrane YPL076W YPL076W GPI2 het Verified 0.962828870473933 0.1678167049107 no 220 0.178965557219839 593 0.002 FT LIPID METABOLISM ENDOPLASMIC RETICULUM Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane|endoplasmic reticulum YLR198C_d YLR198C YLR198C het Dubious 0.960700604457424 0.168351363609915 no 221 0.162575655824378 578 0.005 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Unknown Unknown Unknown YDL139C YDL139C SCM3 het Verified 0.956054101054892 0.169522451103286 no 222 0.158231921138067 579 0.003 FT PROTEIN LOCALIZATION|CELL CYCLE|PROTEOLYSIS|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP G2/M transition of mitotic cell cycle|chromosome segregation|protein localization to chromosome, centromeric region|Unknown centromere-specific nucleosome binding nucleus|condensed nuclear chromosome, centromeric region|cytoplasm YFL005W YFL005W SEC4 het Verified 0.952817231395671 0.170341341045911 no 223 0.158708454812137 586 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION ENDOMEMBRANE SYSTEM|MITOCHONDRION|PLASMA MEMBRANE|CYTOSKELETON Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane bipolar cellular bud site selection|autophagy|exocytosis|Golgi to plasma membrane transport|small GTPase mediated signal transduction|ascospore-type prospore assembly|membrane addition at site of cytokinesis|vesicle fusion|cytokinesis GTP binding|GTPase activity mitochondrial outer membrane|vesicle|incipient cellular bud site|mitochondrion|transport vesicle|actin cap|plasma membrane YML043C YML043C RRN11 het Verified 0.948459210284961 0.171447864368153 no 224 0.135731515069475 478 0.009 FT NUCLEUS Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I CORE element sequence-specific DNA binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity RNA polymerase I core factor complex YNL151C YNL151C RPC31 het Verified 0.939093559246771 0.173841355409308 no 225 0.165113510430487 568 0.001 FT NUCLEUS RNA polymerase III subunit C31 transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YAR019C YAR019C CDC15 het Verified 0.938283515873921 0.17404936437254 no 226 0.172941532250205 596 0.001 FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress regulation of exit from mitosis|protein phosphorylation|meiotic anaphase II|cytokinesis protein kinase activity spindle pole body|cellular bud neck YGR009C YGR009C SEC9 het Verified 0.937585739630084 0.17422867134468 no 227 0.153377818092835 565 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION PLASMA MEMBRANE t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Golgi to plasma membrane transport|vesicle fusion SNAP receptor activity SNARE complex|extrinsic to plasma membrane YOR249C YOR249C APC5 het Verified 0.936622996524626 0.174476259270591 no 228 0.205774425941257 646 0.001 FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress chromatin assembly|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YOL094C YOL094C RFC4 het Verified 0.93537353549963 0.174797915266588 no 229 0.140574430918806 488 0.001 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity Elg1 RFC-like complex|Rad17 RFC-like complex|DNA replication factor C complex|Ctf18 RFC-like complex YJL194W YJL194W CDC6 het Verified 0.933888048691831 0.175180822178812 no 230 0.177710328523416 612 0.002 FT CELL CYCLE|CELL DIVISION|GENE SILENCING NUCLEUS|PLASMA MEMBRANE Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress regulation of DNA-dependent DNA replication initiation|regulation of chromatin silencing at telomere|pre-replicative complex assembly|G1/S transition of mitotic cell cycle DNA replication origin binding|protein binding|chromatin binding|ATPase activity|GTP binding|GTPase activity|ATP binding DNA replication preinitiation complex|pre-replicative complex YPL028W YPL028W ERG10 het Verified 0.932333959544262 0.175581981346952 no 231 0.1518989659274 556 0.006 FT LIPID METABOLISM Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis ergosterol biosynthetic process acetyl-CoA C-acetyltransferase activity cytosol YLR100W YLR100W ERG27 het Verified 0.926487631341679 0.177096310997258 no 232 0.165919496246425 582 0.001 FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs ergosterol biosynthetic process 3-keto sterol reductase activity mitochondrial outer membrane|endoplasmic reticulum|endoplasmic reticulum membrane YPL007C YPL007C TFC8 het Verified 0.925150153701853 0.177443903875919 no 233 0.164648379716203 609 0.007 FT NUCLEUS One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter|transcription from RNA polymerase III promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YJR046W YJR046W TAH11 het Verified 0.918392855360489 0.1792066149097 no 234 0.199113676308706 625 0.003 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION NUCLEUS DNA replication licensing factor, required for pre-replication complex assembly regulation of DNA-dependent DNA replication initiation|double-strand break repair via break-induced replication|pre-replicative complex assembly DNA replication origin binding|ATP binding pre-replicative complex|nucleus|cytoplasm YBR123C YBR123C TFC1 het Verified 0.915705996610449 0.179910559491089 no 235 0.196091142430058 676 0.001 FT NUCLEUS One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YPL142C_d YPL142C YPL142C het Dubious 0.914987513368115 0.180099092669557 no 236 0.201106049587619 700 0.005 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Unknown Unknown Unknown YOR046C YOR046C DBP5 het Verified 0.909818615310246 0.18145908781279 no 237 0.1650721563631 535 0.001 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|SITE OF POLARIZED GROWTH NUCLEAR PORE Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p translational termination|mRNA export from nucleus RNA helicase activity|inositol hexakisphosphate binding|RNA-dependent ATPase activity nuclear pore cytoplasmic filaments|nucleus|polysome|nuclear pore|cellular bud tip|cytoplasm YDR413C_d YDR413C YDR413C het Dubious 0.908336664407037 0.181850188504283 no 238 0.196902652085014 664 0.001 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Unknown Unknown Unknown YGL092W YGL092W NUP145 het Verified 0.907565945966204 0.182053796548955 no 239 0.190697502279016 678 0.003 FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 double-strand break repair|poly(A)+ mRNA export from nucleus|maintenance of chromatin silencing at telomere|posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery|tRNA export from nucleus|protein targeting to nuclear inner membrane|telomere tethering at nuclear periphery|nuclear pore distribution|positive regulation of transcription, DNA-dependent|NLS-bearing substrate import into nucleus|protein import into nucleus nucleocytoplasmic transporter activity|RNA binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore|nuclear pore outer ring YPL083C YPL083C SEN54 het Verified 0.904335269277497 0.182908825246315 no 240 0.179108462235577 615 0.008 FT RNA PROCESSING ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YER006W YER006W NUG1 het Verified 0.896722545731558 0.184933493479989 no 241 0.17580897406485 614 0.004 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus ribosomal large subunit export from nucleus|rRNA processing RNA binding|GTPase activity nucleolus|nucleus|preribosome, large subunit precursor YJL081C YJL081C ARP4 het Verified 0.893003967129659 0.185927524095151 no 242 0.218816887542307 761 0.002 FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME HISTONE ACETYLTRANSFERASE Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes DNA repair|histone acetylation|kinetochore assembly|chromatin organization|regulation of transcription, DNA-dependent|regulation of transcription from RNA polymerase II promoter|chromatin remodeling chromatin binding|histone acetyltransferase activity|ATP-dependent 3'-5' DNA helicase activity nuclear chromatin|Swr1 complex|nucleus|Ino80 complex|NuA4 histone acetyltransferase complex YLR276C YLR276C DBP9 het Verified 0.890915791969623 0.186487173794255 no 243 0.153313095386025 530 0.008 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent DNA helicase activity|ATP-dependent DNA/RNA helicase activity|ATP-dependent RNA helicase activity nucleolus YOR181W YOR181W LAS17 het Verified 0.882528213996993 0.188745614639144 no 244 0.175821939192459 589 0.001 FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) bipolar cellular bud site selection|endocytosis|actin polymerization or depolymerization|actin filament organization|actin cortical patch localization|positive regulation of actin filament bundle assembly|response to osmotic stress|cytokinesis cytoskeletal protein binding mating projection tip|cytoplasm|actin cortical patch YIL118W YIL118W RHO3 het Verified 0.88135055518152 0.189064055445633 no 245 0.175118459177721 555 0.004 FT VESICLE-MEDIATED TRANSPORT|SIGNALING|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p positive regulation of formin-nucleated actin cable assembly|establishment or maintenance of actin cytoskeleton polarity|positive regulation of exocytosis GTP binding|GTPase activity cytosol|plasma membrane|cellular bud YLR005W YLR005W SSL1 het Verified 0.87700934840037 0.190240780219947 no 246 0.223087393512016 805 0.004 FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|TRANSLATION|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p phosphorylation of RNA polymerase II C-terminal domain|translational initiation|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YDL132W YDL132W CDC53 het Verified 0.873257449290774 0.191261383840986 no 247 0.211163128810705 710 0.009 FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|G2/M transition of mitotic cell cycle|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity|DNA replication origin binding|protein binding, bridging SCF ubiquitin ligase complex YJL011C YJL011C RPC17 het Verified 0.864233297768225 0.193729871941521 no 248 0.206503137813399 733 0.004 FT NUCLEUS RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YKR071C YKR071C DRE2 het Verified 0.860180166891015 0.194844867816219 no 249 0.234836679329122 757 0.001 FT COFACTOR METABOLISM MITOCHONDRION Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress iron-sulfur cluster assembly electron carrier activity cytosol|cytoplasm|mitochondrial intermembrane space YGL207W YGL207W SPT16 het Verified 0.858983244497063 0.195174880129419 no 250 0.229075111589192 782 0.001 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome DNA replication-independent nucleosome organization|regulation of transcription by chromatin organization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription initiation from RNA polymerase II promoter|nucleosome assembly|transcription elongation from RNA polymerase II promoter|DNA-dependent DNA replication chromatin binding|nucleosome binding|histone binding nuclear chromatin|FACT complex|replication fork protection complex|alpha DNA polymerase:primase complex|transcription elongation factor complex YGR280C YGR280C PXR1 het Verified 0.858320748341802 0.195357687794741 no 251 0.186454619149006 628 0.013 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain box C/D snoRNA 3'-end processing|negative regulation of telomere maintenance via telomerase telomerase inhibitor activity nucleoplasm|nucleolus YDR170C YDR170C SEC7 het Verified 0.845465470842874 0.198925501748725 no 252 0.199945895513672 676 0.006 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|RESPONSE TO NUTRIENT LEVELS GOLGI APPARATUS Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|autophagic vacuole assembly ARF guanyl-nucleotide exchange factor activity trans-Golgi network|cytosol|late endosome|integral to membrane|Golgi-associated vesicle YBL030C YBL030C PET9 het Verified 0.8399072450946 0.200480197537103 no 253 0.22904322188534 729 0.009 FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication heme transport|transmembrane transport|aerobic respiration|anaerobic respiration|ATP transport|ADP transport|apoptotic process|mitochondrial transport ATP:ADP antiporter activity mitochondrion|integral to membrane|mitochondrial inner membrane YAL025C YAL025C MAK16 het Verified 0.831285338371002 0.202906227614874 no 254 0.20562169448425 718 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|preribosome, large subunit precursor YLR060W YLR060W FRS1 het Verified 0.831218345383946 0.202925146480349 no 255 0.189228209494541 648 0.001 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control phenylalanyl-tRNA aminoacylation phenylalanine-tRNA ligase activity phenylalanine-tRNA ligase complex|cytoplasm YDR292C YDR292C SRP101 het Verified 0.830645181423336 0.203087051438469 no 256 0.166210808766871 639 0.005 FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p SRP-dependent cotranslational protein targeting to membrane|protein targeting to ER GTP binding|signal recognition particle binding endoplasmic reticulum membrane|signal recognition particle receptor complex YPL010W YPL010W RET3 het Verified 0.825915868123265 0.204425909252146 no 257 0.178482029976572 609 0.002 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER molecular_function COPI vesicle coat YJL010C YJL010C NOP9 het Verified 0.82399614891781 0.204970873077473 no 258 0.201778286149483 671 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit export from nucleus RNA binding preribosome, small subunit precursor|nucleolus|nucleus|90S preribosome YER094C YER094C PUP3 het Verified 0.82353352321791 0.205102330828489 no 259 0.261037806086194 866 0.003 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activator activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YDR021W YDR021W FAL1 het Verified 0.820063529856118 0.206089945738646 no 260 0.16977854110438 556 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus|FAl1-SGD1 complex YNR003C YNR003C RPC34 het Verified 0.818423217127028 0.206557784527602 no 261 0.181943861140006 657 0.002 FT NUCLEUS|MITOCHONDRION RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity mitochondrion|DNA-directed RNA polymerase III complex|nucleus|cytoplasm YJR076C YJR076C CDC11 het Verified 0.81610460359934 0.207220155586911 no 262 0.216036394141005 708 0.002 FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells maintenance of cell polarity|cell cycle cytokinesis|positive regulation of protein kinase activity structural molecule activity|GTP binding|1-phosphatidylinositol binding spindle microtubule|septin complex|prospore membrane|ascospore wall|mating projection base|meiotic spindle|cellular bud neck septin ring|septin filament array|cytoplasmic microtubule|mating projection tip YMR005W YMR005W TAF4 het Verified 0.814212257344343 0.207761682370063 no 263 0.223799259246558 716 0.006 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|RNA polymerase II activating transcription factor binding transcription factor TFIID complex YLR141W YLR141W RRN5 het Verified 0.807722026757864 0.209625310271797 no 264 0.172751331963876 585 0.009 FT CHROMATIN ORGANIZATION NUCLEUS Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p transcription of nuclear large rRNA transcript from RNA polymerase I promoter|chromatin organization RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YPL093W YPL093W NOG1 het Verified 0.798949255242465 0.21215991837884 no 265 0.24665287526239 876 0.001 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|rRNA processing GTP binding nucleolus|preribosome, large subunit precursor YBR087W YBR087W RFC5 het Verified 0.798229800924148 0.212368573317269 no 266 0.195090507533455 654 0.005 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|leading strand elongation DNA clamp loader activity Elg1 RFC-like complex|Rad17 RFC-like complex|DNA replication factor C complex|Ctf18 RFC-like complex YDL016C_d YDL016C YDL016C het Dubious 0.79307779740095 0.213866248832401 no 267 0.219788580451456 768 0.000 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Unknown Unknown Unknown YAL033W YAL033W POP5 het Verified 0.792808604558954 0.213944671264738 no 268 0.218230721721677 749 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YJL125C YJL125C GCD14 het Verified 0.788839206675297 0.21510299558892 no 269 0.171107603446314 583 0.002 FT RNA PROCESSING NUCLEUS Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression tRNA methylation tRNA (adenine-N1-)-methyltransferase activity tRNA (m1A) methyltransferase complex|nucleus YPR186C YPR186C PZF1 het Verified 0.787216335611053 0.215577618046887 no 270 0.206268053899785 714 0.000 FT NUCLEUS Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA 5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|TFIIIC-class transcription factor binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIC recruiting transcription factor activity nucleus YML065W YML065W ORC1 het Verified 0.787039484997845 0.215629376184883 no 271 0.170196105514235 542 0.007 FT GENE SILENCING NUCLEUS|CHROMOSOME Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding|ATPase activity|ATP binding DNA replication preinitiation complex|nucleus|pre-replicative complex|nuclear origin of replication recognition complex YHR186C YHR186C KOG1 het Verified 0.780076943728168 0.217672793339061 no 272 0.217345216059452 761 0.015 FT SIGNALING|RESPONSE TO NUTRIENT LEVELS MITOCHONDRION|PLASMA MEMBRANE Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors TOR signaling cascade|regulation of cell growth|cellular response to starvation ubiquitin binding TORC1 complex|fungal-type vacuole membrane|mitochondrion|plasma membrane YBR142W YBR142W MAK5 het Verified 0.764915482649176 0.222160936909735 no 273 0.214995365189235 744 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus YDL148C YDL148C NOP14 het Verified 0.764074948031779 0.222411290211202 no 274 0.234327322969468 799 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit biogenesis|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing snoRNA binding small-subunit processome|mitochondrion|nucleolus|90S preribosome|Noc4p-Nop14p complex YNL103W YNL103W MET4 het Verified 0.761107710463274 0.223296367729901 no 275 0.238401092883719 804 0.000 FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|AMINO ACID METABOLISM NUCLEUS Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p response to cadmium ion|sulfur amino acid metabolic process|response to arsenic-containing substance|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity nucleus YMR227C YMR227C TAF7 het Verified 0.760950283004296 0.223343381647344 no 276 0.235982445636424 812 0.000 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YLR359W YLR359W ADE13 het Verified 0.760508981944582 0.223475201204233 no 277 0.21630602120523 761 0.003 FT NUCLEOTIDE METABOLISM Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency purine nucleotide biosynthetic process|'de novo' IMP biosynthetic process N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity cellular_component YJL050W YJL050W MTR4 het Verified 0.757378919876894 0.224411440822995 no 278 0.21256502310799 738 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|OXIDATION-REDUCTION PROCESS|RNA LOCALIZATION NUCLEUS ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|ncRNA polyadenylation|nuclear polyadenylation-dependent mRNA catabolic process|U4 snRNA 3'-end processing|nuclear mRNA surveillance of mRNA 3'-end processing|nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent 3'-5' RNA helicase activity|poly(A) RNA binding nucleolus|nucleus|TRAMP complex YJR057W YJR057W CDC8 het Verified 0.756506040685533 0.224672926015571 no 279 0.214145817052063 717 0.000 FT NUCLEOTIDE METABOLISM NUCLEUS Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p dTDP biosynthetic process|dTTP biosynthetic process|dUDP biosynthetic process uridylate kinase activity|thymidylate kinase activity nucleus|cytoplasm YFL024C YFL024C EPL1 het Verified 0.756456702173373 0.224687711329558 no 280 0.218769686052678 747 0.002 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS HISTONE ACETYLTRANSFERASE Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb DNA repair|histone acetylation|regulation of transcription from RNA polymerase II promoter histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YNL308C YNL308C KRI1 het Verified 0.75429899298961 0.225334853437683 no 281 0.230836386655935 799 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YKL028W YKL028W TFA1 het Verified 0.749506262224945 0.226776062828853 no 282 0.218117959855227 738 0.000 FT TRANSCRIPTION FROM RNA POL II NUCLEUS|MITOCHONDRION RNA POL II, HOLOENZYME TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening transcriptional open complex formation at RNA polymerase II promoter|transcription from RNA polymerase II promoter single-stranded DNA binding|TFIIH-class transcription factor binding|RNA polymerase II core binding mitochondrion|transcription factor TFIIE complex YLR457C YLR457C NBP1 het Verified 0.749166527227732 0.226878420662008 no 283 0.217147210942088 704 0.002 FT CELL CYCLE|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication establishment of spindle pole body localization to nuclear envelope|spindle pole body duplication associated with nuclear envelope lipid binding integral to nuclear inner membrane|central plaque of spindle pole body|spindle pole body YKL180W YKL180W RPL17A het Verified 0.747060213592969 0.227513607496968 no 284 0.221674273195256 776 0.003 FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit|cytoplasm YOR143C YOR143C THI80 het Verified 0.743795158779591 0.228500204727288 no 285 0.189660781920487 673 0.009 FT COFACTOR METABOLISM Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) thiamine diphosphate biosynthetic process thiamine diphosphokinase activity cytoplasm YDR211W YDR211W GCD6 het Verified 0.73460669696857 0.231289529114144 no 286 0.227264989291573 771 0.013 FT TRANSLATION Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress regulation of translational initiation guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YGR145W YGR145W ENP2 het Verified 0.721258142278812 0.235375352571654 no 287 0.245480229330491 823 0.006 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p ribosomal small subunit biogenesis|rRNA processing molecular_function nucleolus|90S preribosome YOL069W YOL069W NUF2 het Verified 0.715365337094236 0.237191662689432 no 288 0.202432333288622 700 0.004 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton spindle microtubule|Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region|spindle pole body YFL017C YFL017C GNA1 het Verified 0.711466245424693 0.23839768033647 no 289 0.233158267873963 807 0.009 FT CARBOHYDRATE METABOLISM NUCLEUS Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA UDP-N-acetylglucosamine biosynthetic process glucosamine 6-phosphate N-acetyltransferase activity nucleus|cytoplasm YLR166C YLR166C SEC10 het Verified 0.702795369956908 0.24109164153064 no 290 0.269227107374138 914 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion exocytosis|establishment of cell polarity|Golgi to plasma membrane transport|vesicle fusion|vesicle docking involved in exocytosis molecular_function mating projection tip|exocyst YMR240C YMR240C CUS1 het Verified 0.702170243417778 0.241286500196157 no 291 0.245786143818596 843 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p spliceosomal complex assembly|mRNA splicing, via spliceosome molecular_function U2 snRNP|U2-type prespliceosome YBR196C YBR196C PGI1 het Verified 0.700508868946284 0.241804784196616 no 292 0.225285564484102 803 0.006 FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation gluconeogenesis|pentose-phosphate shunt|glycolysis glucose-6-phosphate isomerase activity cytosol|mitochondrion|plasma membrane YPL126W YPL126W NAN1 het Verified 0.694360877345658 0.243727961023979 no 293 0.225976873849052 788 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|RENT complex|90S preribosome YPL143W YPL143W RPL33A het Verified 0.689636229116121 0.245211489109719 no 294 0.249825941013871 880 0.008 FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YIL147C YIL147C SLN1 het Verified 0.681930225353344 0.247641536047896 no 295 0.224370951306296 764 0.000 FT SIGNALING|PROTEIN PHOSPHORYLATION PLASMA MEMBRANE Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators negative regulation of protein kinase activity|protein autophosphorylation|peptidyl-histidine phosphorylation|osmosensory signaling pathway via two-component system osmosensor activity|protein histidine kinase activity|histidine phosphotransfer kinase activity plasma membrane|integral to plasma membrane YGR190C_d YGR190C YGR190C het Dubious 0.681887302690354 0.247655107407311 no 296 0.241347157917549 812 0.008 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Unknown Unknown Unknown YOR159C YOR159C SME1 het Verified 0.674261058624758 0.250072678428288 no 297 0.222722248960553 755 0.003 FT RNA PROCESSING NUCLEUS Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YDL103C YDL103C QRI1 het Verified 0.670994072512156 0.25111215281094 no 298 0.2680198935886 946 0.001 FT CARBOHYDRATE METABOLISM NUCLEUS UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress UDP-N-acetylglucosamine biosynthetic process UDP-N-acetylglucosamine diphosphorylase activity nucleus|cytoplasm YER136W YER136W GDI1 het Verified 0.670044049531628 0.251414855080836 no 299 0.206724741865436 714 0.007 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins vesicle-mediated transport Rab GDP-dissociation inhibitor activity cellular_component YOR057W YOR057W SGT1 het Verified 0.663327518500553 0.25356040793689 no 300 0.249731412032838 844 0.006 FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|SIGNALING UBIQUITIN LIGASE COMPLEX Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress regulation of cell cycle|kinetochore assembly|cAMP-mediated signaling|protein complex assembly|protein ubiquitination protein binding, bridging|chaperone binding ubiquitin ligase complex YKL144C YKL144C RPC25 het Verified 0.65744583606622 0.255447143690686 no 301 0.227713660807591 778 0.001 FT NUCLEUS RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p transcription initiation from RNA polymerase III promoter|tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YPL151C YPL151C PRP46 het Verified 0.656937494119816 0.255610554178017 no 302 0.211428108935212 724 0.004 FT CELL CYCLE|RNA PROCESSING NUCLEUS Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs mRNA splicing, via spliceosome molecular_function Prp19 complex|spliceosomal complex YDR526C_d YDR526C YDR526C het Dubious 0.65253245594894 0.257028871218271 no 303 0.312071182575576 1054 0.000 FF Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YPR108W YPR108W RPN7 het Verified 0.652449334635868 0.257055673591218 no 304 0.28103000110563 928 0.024 FT PROTEOLYSIS NUCLEUS Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits ubiquitin-dependent protein catabolic process structural molecule activity nucleus|proteasome regulatory particle, lid subcomplex YMR290C YMR290C HAS1 het Verified 0.6285826162786 0.264811171997099 no 305 0.252309682752082 826 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS ENDOMEMBRANE SYSTEM|NUCLEUS ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles rRNA processing RNA binding|RNA-dependent ATPase activity|ATP-dependent RNA helicase activity nuclear envelope|nucleolus|preribosome, large subunit precursor|90S preribosome YDR356W YDR356W SPC110 het Verified 0.626547732531618 0.265477869422296 no 306 0.246409405681301 852 0.001 FT CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner karyogamy involved in conjugation with cellular fusion|microtubule nucleation structural constituent of cytoskeleton central plaque of spindle pole body|inner plaque of spindle pole body YLL018C YLL018C DPS1 het Verified 0.625346965693221 0.265871680797689 no 307 0.310893607183837 1062 0.000 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION NUCLEUS Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation cytoplasmic translation|aspartyl-tRNA aminoacylation RNA binding|aspartate-tRNA ligase activity nucleus|cytoplasm YIR008C YIR008C PRI1 het Verified 0.625097499345957 0.265953534543785 no 308 0.23686573816999 828 0.008 FT NUCLEUS|CHROMOSOME Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA replication initiation|DNA replication single-stranded DNA binding|DNA primase activity alpha DNA polymerase:primase complex|nuclear replication fork YBR211C YBR211C AME1 het Verified 0.616826660054811 0.268674532621861 no 309 0.242906724881959 853 0.001 FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress attachment of spindle microtubules to kinetochore|protein localization to kinetochore molecular_function COMA complex|spindle pole body|kinetochore YGL001C YGL001C ERG26 het Verified 0.615365163992901 0.269156795914485 no 310 0.247797526800578 863 0.002 FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis ergosterol biosynthetic process C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity endoplasmic reticulum|endoplasmic reticulum membrane YBR038W YBR038W CHS2 het Verified 0.613378024282599 0.269813206633302 no 311 0.285742538296849 960 0.007 FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM SITE OF POLARIZED GROWTH Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis actomyosin contractile ring contraction|cytokinesis chitin synthase activity integral to membrane|cellular bud neck YBL084C YBL084C CDC27 het Verified 0.606534183741989 0.272080045006405 no 312 0.293289149769729 990 0.002 FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YDR050C YDR050C TPI1 het Verified 0.604219073875349 0.272849000673636 no 313 0.264599327223902 904 0.002 FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease glycolysis triose-phosphate isomerase activity mitochondrion|plasma membrane|cytoplasm YMR093W YMR093W UTP15 het Verified 0.60180742747382 0.273651165230336 no 314 0.308301264194261 994 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|rDNA heterochromatin|nucleolus|t-UTP complex|90S preribosome YBL074C YBL074C AAR2 het Verified 0.599204287172526 0.274518332118345 no 315 0.278086480002259 948 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron assembly of spliceosomal tri-snRNP molecular_function U5 snRNP YJL203W YJL203W PRP21 het Verified 0.597683319392431 0.27502562880882 no 316 0.262583937997548 877 0.007 FT RNA PROCESSING NUCLEUS Subunit of the SF3a splicing factor complex, required for spliceosome assembly mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YLR088W YLR088W GAA1 het Verified 0.590694479868304 0.277362573640883 no 317 0.27663041308724 963 0.004 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|GPI-anchor transamidase complex YNL075W YNL075W IMP4 het Verified 0.587106323951867 0.278566150299877 no 318 0.256486259272804 878 0.010 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs rRNA processing snoRNA binding|single-stranded telomeric DNA binding|rRNA primary transcript binding small-subunit processome|Mpp10 complex|90S preribosome YJR041C YJR041C URB2 het Verified 0.576965371379123 0.281981424235757 no 319 0.248777461837223 844 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis rRNA metabolic process|ribosome biogenesis molecular_function nucleolus YNL263C YNL263C YIF1 het Verified 0.575350000062706 0.282527306151744 no 320 0.272430213929338 876 0.000 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 ER to Golgi vesicle-mediated transport molecular_function integral to membrane|integral to Golgi membrane|endoplasmic reticulum membrane|ER to Golgi transport vesicle YOL120C YOL120C RPL18A het Verified 0.575212950784495 0.282573642542713 no 321 0.311145882381794 1073 0.005 FF|FT TRANSLATION RIBOSOME Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication cytoplasmic translation structural constituent of ribosome cytosolic large ribosomal subunit YOR151C YOR151C RPB2 het Verified 0.570399203180517 0.284203484946849 no 322 0.295234813977602 995 0.002 FT TRANSCRIPTION FROM RNA POL II NUCLEUS|MITOCHONDRION RNA POL II, HOLOENZYME RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity mitochondrion|DNA-directed RNA polymerase II, core complex YGL044C YGL044C RNA15 het Verified 0.568198836945012 0.284949980559322 no 323 0.275653566649877 924 0.006 FT RNA PROCESSING NUCLEUS Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping mRNA cleavage|mRNA polyadenylation mRNA binding|protein heterodimerization activity mRNA cleavage factor complex YJL174W YJL174W KRE9 het Verified 0.562341297326819 0.286941753216311 no 324 0.283769320245395 984 0.001 FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects (1->6)-beta-D-glucan metabolic process|fungal-type cell wall organization molecular_function extracellular region YOR074C YOR074C CDC21 het Verified 0.561242777064784 0.287316022527882 no 325 0.299073082898342 1032 0.004 FT NUCLEOTIDE METABOLISM NUCLEUS Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S dTMP biosynthetic process thymidylate synthase activity nucleus YBR029C YBR029C CDS1 het Verified 0.557312536752842 0.288656955806112 no 326 0.335564857630181 1084 0.004 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids phosphatidylserine metabolic process|phosphatidylglycerol biosynthetic process phosphatidate cytidylyltransferase activity mitochondrion|integral to membrane|endoplasmic reticulum YDR267C YDR267C CIA1 het Verified 0.553060656773306 0.290110937952903 no 327 0.280816071663074 988 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|COFACTOR METABOLISM NUCLEUS Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein iron-sulfur cluster assembly|ribosomal large subunit export from nucleus|tRNA wobble uridine modification molecular_function cytosol|nucleus|cytoplasm YBR080C YBR080C SEC18 het Verified 0.549917661820994 0.291187924800227 no 328 0.266166111588133 906 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RESPONSE TO NUTRIENT LEVELS SITE OF POLARIZED GROWTH ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Golgi vesicle docking|SNARE complex disassembly|Golgi to plasma membrane protein transport|vesicle fusion with Golgi apparatus|vacuole fusion, non-autophagic|inter-Golgi cisterna vesicle-mediated transport|autophagic vacuole assembly ATPase activity mating projection tip|cytoplasm YGR274C YGR274C TAF1 het Verified 0.54622299688789 0.292456330040449 no 329 0.294148967913027 1017 0.004 FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter TBP-class protein binding|chromatin binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly|histone acetyltransferase activity transcription factor TFIID complex YBL014C YBL014C RRN6 het Verified 0.541921101635885 0.293936429678379 no 330 0.29095750505335 988 0.007 FT NUCLEUS Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p transcription initiation from RNA polymerase I promoter|transcription of nuclear large rRNA transcript from RNA polymerase I promoter RNA polymerase I regulatory region DNA binding|RNA polymerase I transcription factor binding|RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity RNA polymerase I core factor complex YDR362C YDR362C TFC6 het Verified 0.534651575587761 0.296445406196575 no 331 0.284340975411972 985 0.002 FT NUCLEUS One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity transcription factor TFIIIC complex YLR440C YLR440C SEC39 het Verified 0.532727084278881 0.297111257356961 no 332 0.27852607574866 952 0.003 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope retrograde vesicle-mediated transport, Golgi to ER|vesicle-mediated transport|ER-dependent peroxisome organization molecular_function Dsl1p complex|nuclear envelope|endoplasmic reticulum|peroxisome|endoplasmic reticulum membrane YOL133W YOL133W HRT1 het Verified 0.529474616198714 0.29823812435193 no 333 0.303587488131066 1028 0.001 FT CELL CYCLE|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity SCF ubiquitin ligase complex|nucleus|cytoplasm YJL039C YJL039C NUP192 het Verified 0.528474834998812 0.298584904417287 no 334 0.310877891496067 1035 0.001 FT PROTEIN LOCALIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 nucleocytoplasmic transport|nuclear pore organization structural constituent of nuclear pore integral to membrane|nuclear pore nuclear basket|nuclear pore|nuclear pore inner ring YHL015W YHL015W RPS20 het Verified 0.527616400890252 0.298882803649655 no 335 0.334467632966603 1104 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) structural constituent of ribosome cytosolic small ribosomal subunit YIL075C YIL075C RPN2 het Verified 0.527553227029123 0.29890473197563 no 336 0.265420602715311 912 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress proteasome assembly|ubiquitin-dependent protein catabolic process endopeptidase activity|protein binding, bridging proteasome regulatory particle, base subcomplex|nucleus|proteasome storage granule YBR153W YBR153W RIB7 het Verified 0.523053550519009 0.300468494103471 no 337 0.28235758260026 922 0.005 FT OXIDATION-REDUCTION PROCESS Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway riboflavin biosynthetic process 5-amino-6-(5-phosphoribosylamino)uracil reductase activity cellular_component YFR002W YFR002W NIC96 het Verified 0.517740126874758 0.302319798659015 no 338 0.350736306797468 1212 0.001 FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore organization structural constituent of nuclear pore integral to membrane|nuclear pore nuclear basket|nuclear pore linkers|nuclear pore YPL085W YPL085W SEC16 het Verified 0.516426386548706 0.302778320270361 no 339 0.287739072958784 966 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p COPII vesicle coating protein anchor ER to Golgi transport vesicle membrane YLR009W YLR009W RLP24 het Verified 0.512785626186818 0.304050642731945 no 340 0.345885831172006 1142 0.002 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp ribosomal large subunit biogenesis molecular_function nucleolus|preribosome, large subunit precursor YML031W YML031W NDC1 het Verified 0.510377708947685 0.304893435266387 no 341 0.312197379806218 1065 0.017 FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON NUCLEAR PORE Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 nucleocytoplasmic transport|nuclear pore organization|spindle pole body duplication associated with nuclear envelope structural constituent of nuclear pore integral to membrane|nuclear pore transmembrane ring|spindle pole body|nuclear pore YGR114C_d YGR114C YGR114C het Dubious 0.493513763463802 0.310824808625156 no 342 0.385713397874815 1277 0.010 FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Unknown Unknown Unknown YBR190W_d YBR190W YBR190W het Dubious 0.483051367583722 0.314529631851599 no 343 0.328932544995174 1089 0.001 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Unknown Unknown Unknown YGL142C YGL142C GPI10 het Verified 0.481953603985926 0.314919452750561 no 344 0.295253249338632 997 0.004 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog GPI anchor biosynthetic process dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to membrane YLL004W YLL004W ORC3 het Verified 0.478383578186592 0.316188609804097 no 345 0.303908086276942 1064 0.000 FT GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette chromatin binding|DNA replication origin binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YPL169C YPL169C MEX67 het Verified 0.477884066803018 0.316366361190864 no 346 0.368970324419925 1246 0.002 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP mRNA export from nucleus|ribosomal large subunit export from nucleus protein binding|RNA binding|structural constituent of nuclear pore integral to membrane|nuclear RNA export factor complex|nuclear pore|cytoplasm YDR464W YDR464W SPP41 het Verified 0.475505146152556 0.317213483058868 no 347 0.342087838417953 1169 0.012 FT TRANSCRIPTION FROM RNA POL II NUCLEUS Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 negative regulation of transcription from RNA polymerase II promoter molecular_function nucleus YDR041W YDR041W RSM10 het Verified 0.463092336399138 0.321649091470827 no 348 0.311224058724606 1038 0.001 FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YFR051C YFR051C RET2 het Verified 0.461911040026002 0.322072558327708 no 349 0.320537712538059 1088 0.013 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER retrograde vesicle-mediated transport, Golgi to ER|Golgi inheritance|ER to Golgi vesicle-mediated transport|Golgi localization molecular_function COPI vesicle coat YCL054W YCL054W SPB1 het Verified 0.449355522734351 0.326587605398723 no 350 0.338454288018859 1155 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA methylation rRNA (uridine-2'-O-)-methyltransferase activity|rRNA (guanine) methyltransferase activity nucleolus|nucleus|preribosome, large subunit precursor YHR020W YHR020W YHR020W het Verified 0.442591404816546 0.329030651440263 no 351 0.314478253546429 1032 0.004 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments chromosome organization|tRNA aminoacylation for protein translation proline-tRNA ligase activity ribosome YDR478W YDR478W SNM1 het Verified 0.438731231863345 0.330428147011173 no 352 0.342199918066314 1144 0.014 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP mRNA cleavage|rRNA processing ribonuclease MRP activity|RNA binding ribonuclease MRP complex YDL108W YDL108W KIN28 het Verified 0.424840657054168 0.335476418544797 no 353 0.330180020461196 1121 0.001 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase I promoter|protein phosphorylation|transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activating kinase activity|protein kinase activity TFIIK complex|holo TFIIH complex YMR298W YMR298W LIP1 het Verified 0.423425682343084 0.335992355131247 no 354 0.32628339899195 1073 0.006 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis ceramide biosynthetic process sphingosine N-acyltransferase activity nuclear envelope|endoplasmic reticulum|endoplasmic reticulum membrane YJL074C YJL074C SMC3 het Verified 0.417689212078555 0.338087180386808 no 355 0.302758626972613 1033 0.003 FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member synaptonemal complex assembly|ascospore formation|reciprocal meiotic recombination|mitotic sister chromatid cohesion|meiotic sister chromatid cohesion ATPase activity nucleus|nuclear mitotic cohesin complex YOR353C YOR353C SOG2 het Verified 0.415056104647649 0.339050416043684 no 356 0.366079272365005 1250 0.002 FT CELL CYCLE|SIGNALING|CELL DIVISION SITE OF POLARIZED GROWTH Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis signal transduction|cytokinetic cell separation|budding cell apical bud growth molecular_function incipient cellular bud site|cellular bud|mating projection tip|cytoplasm YNL178W YNL178W RPS3 het Verified 0.413068758934814 0.339778118824329 no 357 0.372224528952816 1244 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION|RNA LOCALIZATION RIBOSOME Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 cytoplasmic translation|rRNA export from nucleus structural constituent of ribosome|DNA-(apurinic or apyrimidinic site) lyase activity preribosome, small subunit precursor|cytosolic small ribosomal subunit|90S preribosome YIL022W YIL022W TIM44 het Verified 0.411658875090242 0.340294735954473 no 358 0.321848398896088 1064 0.001 FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) protein import into mitochondrial matrix protein binding, bridging|chaperone binding mitochondrion|integral to membrane|presequence translocase-associated import motor YDR172W YDR172W SUP35 het Verified 0.410973050907495 0.340546147781903 no 359 0.348949483925008 1183 0.002 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype translational termination|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay translation release factor activity cytosol|translation release factor complex YPR183W YPR183W DPM1 het Verified 0.408632069371773 0.341404846210558 no 360 0.358313874673676 1184 0.004 FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation GPI anchor biosynthetic process|protein O-linked glycosylation|protein N-linked glycosylation dolichyl-phosphate beta-D-mannosyltransferase activity mitochondrial outer membrane|mitochondrion|endoplasmic reticulum|nuclear outer membrane-endoplasmic reticulum membrane network YNL261W YNL261W ORC5 het Verified 0.404752163298469 0.342829847402242 no 361 0.320288067599062 1099 0.005 FT GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing regulation of chromatin silencing at telomere|DNA replication initiation|pre-replicative complex assembly|chromatin silencing at silent mating-type cassette DNA replication origin binding|ATP binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YJL076W YJL076W NET1 het Verified 0.400132726412555 0.344529380512734 no 362 0.353435952563557 1171 0.000 FT CELL CYCLE|CELL DIVISION|GENE SILENCING NUCLEUS Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication regulation of exit from mitosis|nucleolus organization|chromatin silencing at rDNA rDNA binding nucleolus|RENT complex YJR112W YJR112W NNF1 het Verified 0.399998171858572 0.344578931639822 no 363 0.336421305578504 1107 0.003 FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore YHR069C YHR069C RRP4 het Verified 0.396792721172994 0.345760159362731 no 364 0.40391992677857 1330 0.003 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription|polyadenylation-dependent snoRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YHR040W YHR040W BCD1 het Verified 0.393966346926819 0.346802942678134 no 365 0.319698309968784 1075 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein required for the accumulation of box C/D snoRNA snoRNA metabolic process nucleic acid binding nucleus|cytoplasm YDR376W YDR376W ARH1 het Verified 0.389260828288094 0.348541605080045 no 366 0.336148571998594 1139 0.001 FT KETONE METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability ubiquinone biosynthetic process|cellular iron ion homeostasis NADPH-adrenodoxin reductase activity mitochondrion|mitochondrial inner membrane YMR277W YMR277W FCP1 het Verified 0.388340084814564 0.348882188472169 no 367 0.371225570259864 1238 0.001 FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) dephosphorylation of RNA polymerase II C-terminal domain|regulation of transcription from RNA polymerase II promoter protein serine/threonine phosphatase activity nucleus YDL163W_d YDL163W YDL163W het Dubious 0.38768118856689 0.349125989249979 no 368 0.353953401879832 1187 0.001 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Unknown Unknown Unknown YNL112W YNL112W DBP2 het Verified 0.386822757236263 0.349443714204286 no 369 0.368578850939257 1245 0.001 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites nuclear-transcribed mRNA catabolic process, nonsense-mediated decay|rRNA processing RNA helicase activity mitochondrion|nucleus|cytoplasm YDR189W YDR189W SLY1 het Verified 0.385550778496049 0.349914696417624 no 370 0.351367730100774 1212 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|positive regulation of SNARE complex assembly SNARE binding|syntaxin binding SNARE complex|endoplasmic reticulum|ER to Golgi transport vesicle|Golgi membrane YPR025C YPR025C CCL1 het Verified 0.384934150320884 0.350143101740808 no 371 0.352138117798069 1174 0.003 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|PROTEIN PHOSPHORYLATION NUCLEUS|MITOCHONDRION RNA POL II, HOLOENZYME Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|cyclin-dependent protein kinase regulator activity mitochondrion|holo TFIIH complex|TFIIK complex YKR083C YKR083C DAD2 het Verified 0.382025091213018 0.351221376754333 no 372 0.379677476363477 1256 0.000 FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton DASH complex|condensed nuclear chromosome kinetochore|mitotic spindle YBR121C YBR121C GRS1 het Verified 0.381609372121655 0.351375565740427 no 373 0.350355808427223 1184 0.005 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication mitochondrial glycyl-tRNA aminoacylation|glycyl-tRNA aminoacylation|DNA-dependent transcription, termination glycine-tRNA ligase activity mitochondrion|cytoplasm YHR024C YHR024C MAS2 het Verified 0.376782968249841 0.353167449450266 no 374 0.373947456436458 1204 0.000 FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion|mitochondrial processing peptidase complex YNL317W YNL317W PFS2 het Verified 0.376404394762669 0.353308139644493 no 375 0.376858144318722 1255 0.002 FT RNA PROCESSING NUCLEUS Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YDR118W YDR118W APC4 het Verified 0.374189370360986 0.354131715875702 no 376 0.348603855941334 1160 0.004 FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YDR212W YDR212W TCP1 het Verified 0.370675434228777 0.355439644771949 no 377 0.38732181311505 1296 0.003 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein protein folding unfolded protein binding plasma membrane|chaperonin-containing T-complex YOL066C YOL066C RIB2 het Verified 0.368111157314475 0.356395175898963 no 378 0.35791771415695 1179 0.002 FT RNA PROCESSING Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs riboflavin biosynthetic process|tRNA pseudouridine synthesis pseudouridine synthase activity|2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity cytoplasm YER082C YER082C UTP7 het Verified 0.366031551178149 0.357170766200289 no 379 0.364542469769515 1229 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YKL013C YKL013C ARC19 het Verified 0.361718710530907 0.358781121775634 no 380 0.366138355550342 1229 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin cortical patch assembly structural molecule activity Arp2/3 protein complex YKL033W YKL033W TTI1 het Verified 0.357038390829529 0.360531532780816 no 381 0.37696628671552 1271 0.004 FT CHROMATIN ORGANIZATION NUCLEUS|MITOCHONDRION|CHROMOSOME Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies biological_process molecular_function ASTRA complex|mitochondrion|cytoplasm YLR438C-A YLR438C-A LSM3 het Verified 0.353181827279169 0.361976065433461 no 382 0.36276993099688 1232 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YNL313C YNL313C EMW1 het Verified 0.352448305811253 0.362251040128808 no 383 0.35137338262087 1164 0.005 FT CELL WALL ORG/BIOGENESIS NUCLEUS Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress fungal-type cell wall organization molecular_function nucleus|cytoplasm YMR281W YMR281W GPI12 het Verified 0.347167280086639 0.36423282531128 no 384 0.359457770117591 1216 0.000 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp GPI anchor biosynthetic process N-acetylglucosaminylphosphatidylinositol deacetylase activity integral to membrane|endoplasmic reticulum membrane YDR324C YDR324C UTP4 het Verified 0.346948565555403 0.364314980025908 no 385 0.383722962719116 1303 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|t-UTP complex|90S preribosome YGL120C YGL120C PRP43 het Verified 0.344207735112867 0.365345032779359 no 386 0.363166086097018 1177 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|spliceosomal complex disassembly|ribosomal large subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA|rRNA processing ATP-dependent RNA helicase activity mitochondrion|post-mRNA release spliceosomal complex|90S preribosome YIL150C YIL150C MCM10 het Verified 0.343487262390274 0.365615960874002 no 387 0.384076777491605 1286 0.001 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|double-strand break repair via break-induced replication|DNA replication initiation|chromatin silencing at telomere|chromatin silencing at silent mating-type cassette double-stranded DNA binding|protein binding|DNA replication origin binding|single-stranded DNA binding replication fork protection complex|replication fork|nucleus YGL103W YGL103W RPL28 het Verified 0.342601090787633 0.365949290676806 no 388 0.383042458383699 1270 0.001 FT TRANSLATION NUCLEUS RIBOSOME Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance cytoplasmic translation structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit|nucleus YBR202W YBR202W MCM7 het Verified 0.341716956149385 0.366281955150699 no 389 0.392885599890933 1291 0.000 FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex S phase of mitotic cell cycle|regulation of transcription from RNA polymerase II promoter|DNA replication initiation|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|pre-replicative complex assembly|double-strand break repair via break-induced replication|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere DNA replication origin binding|single-stranded DNA-dependent ATPase activity|transcription cofactor activity|single-stranded DNA binding|ATP-dependent 3'-5' DNA helicase activity|ATP binding|four-way junction helicase activity|DNA helicase activity|chromatin binding replication fork protection complex|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YMR112C YMR112C MED11 het Verified 0.341494194592407 0.366365787270781 no 390 0.329883221741828 1109 0.001 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein negative regulation of transcription from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter TFIIH-class transcription factor binding|TFIIH-class transcription factor recruiting transcription factor activity core mediator complex YGR218W YGR218W CRM1 het Verified 0.340654431121004 0.36668187375738 no 391 0.358276492217563 1188 0.002 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin mRNA export from nucleus|protein export from nucleus|protein localization to kinetochore|ribosomal large subunit export from nucleus protein transporter activity integral to membrane|nucleus|spindle pole body|kinetochore YGR065C YGR065C VHT1 het Verified 0.33851371012076 0.367488048559147 no 392 0.359519473404599 1192 0.008 FT PLASMA MEMBRANE High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin biotin transport biotin transporter activity integral to membrane|plasma membrane YBL023C YBL023C MCM2 het Verified 0.33088897885669 0.370364173549263 no 393 0.342612502239461 1175 0.009 FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|pre-replicative complex assembly|double-strand break repair via break-induced replication chromatin binding|DNA replication origin binding|DNA helicase activity|single-stranded DNA binding replication fork protection complex|nucleus|DNA replication preinitiation complex|pre-replicative complex|cytoplasm|MCM complex YPL175W YPL175W SPT14 het Verified 0.322308817641277 0.373609376884749 no 394 0.386810772004195 1307 0.002 FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins GPI anchor biosynthetic process UDP-glycosyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|endoplasmic reticulum YGR091W YGR091W PRP31 het Verified 0.320209828486851 0.374404636545503 no 395 0.363712758706073 1212 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS|MITOCHONDRION Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome|spliceosomal snRNP assembly molecular_function mitochondrion|U4/U6 x U5 tri-snRNP complex YGR186W YGR186W TFG1 het Verified 0.316395638708676 0.375851113438929 no 396 0.390643682681092 1291 0.002 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 transcription initiation from RNA polymerase II promoter|transcriptional start site selection at RNA polymerase II promoter|dephosphorylation of RNA polymerase II C-terminal domain|promoter clearance from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly|transcription elongation from RNA polymerase II promoter|regulation of protein serine/threonine phosphatase activity core RNA polymerase II binding transcription factor activity|protein phosphatase activator activity|core RNA polymerase II recruiting transcription factor activity transcription factor TFIIF complex YGL047W YGL047W ALG13 het Verified 0.314243501833686 0.376668052579039 no 397 0.370125165337603 1234 0.004 FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress dolichol-linked oligosaccharide biosynthetic process N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity cytosol|UDP-N-acetylglucosamine transferase complex|extrinsic to endoplasmic reticulum membrane|endoplasmic reticulum|nucleus|cytoplasm YBR088C YBR088C POL30 het Verified 0.310216422004025 0.378198191589313 no 398 0.367514470000739 1221 0.001 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION|GENE SILENCING NUCLEUS|CHROMOSOME Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair lagging strand elongation|postreplication repair|base-excision repair|nucleotide-excision repair|mismatch repair|meiotic mismatch repair|leading strand elongation|chromatin silencing at telomere|mitotic sister chromatid cohesion|chromatin silencing at silent mating-type cassette DNA polymerase processivity factor activity PCNA complex|nucleus|replication fork YNR011C YNR011C PRP2 het Verified 0.309598745083655 0.378433055357321 no 399 0.385757458107452 1288 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 generation of catalytic spliceosome for first transesterification step RNA-dependent ATPase activity U2-type catalytic step 1 spliceosome YOR102W_d YOR102W YOR102W het Dubious 0.307138864286505 0.379368838486562 no 400 0.387952289937518 1270 0.000 Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Unknown Unknown Unknown YOR310C YOR310C NOP58 het Verified 0.307049678650965 0.37940277963209 no 401 0.390632700205095 1303 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function box C/D snoRNP complex|small-subunit processome|nucleolus|90S preribosome YDR188W YDR188W CCT6 het Verified 0.306634238837896 0.379560894726905 no 402 0.394219321704421 1310 0.003 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif protein folding unfolded protein binding chaperonin-containing T-complex YKL006C-A YKL006C-A SFT1 het Verified 0.303804449850706 0.380638438197571 no 403 0.36362550584911 1215 0.000 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment intra-Golgi vesicle-mediated transport|vesicle fusion SNAP receptor activity SNARE complex|Golgi membrane YML077W YML077W BET5 het Verified 0.303742317958344 0.380662107566311 no 404 0.371664375616357 1231 0.011 FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM|GOLGI APPARATUS Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YJR023C_d YJR023C YJR023C het Dubious 0.29270536301432 0.384873685129552 no 405 0.364196397648737 1210 0.004 Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Unknown Unknown Unknown YGR278W YGR278W CWC22 het Verified 0.288507896790269 0.386478993310598 no 406 0.355489795625399 1197 0.003 FT RNA PROCESSING NUCLEUS Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex|U2-type catalytic step 1 spliceosome YPL128C YPL128C TBF1 het Verified 0.285265772436212 0.387720266746037 no 407 0.390303979212076 1257 0.002 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|GENE SILENCING NUCLEUS|CHROMOSOME Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation telomere maintenance via telomere lengthening|negative regulation of chromatin silencing|snoRNA transcription from an RNA polymerase II promoter|negative regulation of telomere maintenance via telomerase RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|chromatin insulator sequence binding|telomeric DNA binding nuclear chromosome, telomeric region|nucleus YOR169C_d YOR169C YOR169C het Dubious 0.283129276057474 0.388538870160952 no 408 0.365668392778361 1239 0.000 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Unknown Unknown Unknown YNL216W YNL216W RAP1 het Verified 0.28309261170999 0.388552922521443 no 409 0.397976570487055 1304 0.003 FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY|GENE SILENCING NUCLEUS|CHROMOSOME Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) negative regulation of chromatin silencing|establishment of protein localization to chromatin|establishment of chromatin silencing at telomere|regulation of transcription by chromatin organization|protection from non-homologous end joining at telomere|negative regulation of transcription from RNA polymerase II promoter|telomere maintenance via telomere lengthening|telomere maintenance|regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter|establishment of protein localization to telomere|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere core promoter proximal region sequence-specific DNA binding|DNA binding, bending|sequence-specific DNA binding|TFIID-class transcription factor binding|TBP-class protein binding RNA polymerase II transcription factor activity|telomeric DNA binding|RNA polymerase II transcription factor binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity|G-quadruplex DNA binding|nucleosomal DNA binding nuclear chromosome|nuclear nucleosome|nuclear chromosome, telomeric region|nuclear telomere cap complex YLL011W YLL011W SOF1 het Verified 0.28030236753238 0.389622767267897 no 410 0.363394403518912 1187 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|90S preribosome YLR316C YLR316C TAD3 het Verified 0.279655452281603 0.389870929489991 no 411 0.396546183768579 1313 0.004 FT RNA PROCESSING NUCLEUS Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs tRNA modification tRNA-specific adenosine deaminase activity nucleus|cytoplasm YGR246C YGR246C BRF1 het Verified 0.275925844494583 0.391302511576574 no 412 0.36409709306977 1213 0.000 FT TRANSLATION NUCLEUS TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB DNA-dependent transcriptional open complex formation|transposon integration|RNA polymerase III transcriptional preinitiation complex assembly|negative regulation of protein homodimerization activity TFIIIC-class transcription factor binding|RNA polymerase III core binding|DNA binding|TFIIIB-type transcription factor activity transcription factor TFIIIB complex YOR146W_d YOR146W YOR146W het Dubious 0.27589586822351 0.39131402374006 no 413 0.388395809063902 1293 0.007 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Unknown Unknown Unknown YPR181C YPR181C SEC23 het Verified 0.269295734671834 0.393851057113063 no 414 0.420597526853416 1394 0.001 FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p regulation of COPII vesicle coating GTPase activator activity COPII vesicle coat YNL039W YNL039W BDP1 het Verified 0.268762713620071 0.394056144058687 no 415 0.400365093602112 1334 0.000 FT NUCLEUS Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs DNA-dependent transcriptional open complex formation|positive regulation of transposon integration|RNA polymerase III transcriptional preinitiation complex assembly TFIIIC-class transcription factor binding|TFIIIB-type transcription factor activity transcription factor TFIIIB complex YCL043C YCL043C PDI1 het Verified 0.268414225453481 0.394190245408167 no 416 0.379540256654745 1247 0.000 FT ENDOPLASMIC RETICULUM Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication protein folding protein disulfide oxidoreductase activity|protein disulfide isomerase activity endoplasmic reticulum lumen YPL210C YPL210C SRP72 het Verified 0.268372538483665 0.394206287765677 no 417 0.402186484531945 1356 0.001 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding signal recognition particle, endoplasmic reticulum targeting YDL212W YDL212W SHR3 het Verified 0.267230882878012 0.394645699696345 no 418 0.403828130870027 1359 0.002 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface protein folding|ER to Golgi vesicle-mediated transport unfolded protein binding integral to endoplasmic reticulum membrane|integral to membrane|mating projection tip YER159C YER159C BUR6 het Verified 0.265670508555259 0.395246489066736 no 419 0.386347619760805 1263 0.000 FT TRANSCRIPTION FROM RNA POL II NUCLEUS Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity|transcription corepressor activity nucleus|negative cofactor 2 complex YJL014W YJL014W CCT3 het Verified 0.265205500885832 0.395425578747474 no 420 0.425877762008107 1399 0.004 FF Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YGR030C YGR030C POP6 het Verified 0.261340472950509 0.39691497891156 no 421 0.395437129930468 1330 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YPR137W YPR137W RRP9 het Verified 0.260042410618526 0.397415529818184 no 422 0.416590479191096 1372 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein rRNA modification|rRNA processing snoRNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|90S preribosome YGR185C YGR185C TYS1 het Verified 0.257572306097948 0.398368502606919 no 423 0.354369807666389 1143 0.000 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION NUCLEUS Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress tyrosyl-tRNA aminoacylation tyrosine-tRNA ligase activity nucleus|cytoplasm YPL218W YPL218W SAR1 het Verified 0.257490920277881 0.398399911819929 no 424 0.392019453783128 1279 0.003 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport regulation of COPII vesicle coating GTPase activity COPII vesicle coat YPR112C YPR112C MRD1 het Verified 0.254420508985286 0.399585354127109 no 425 0.403835998304973 1330 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|small-subunit processome assembly|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|90S preribosome YER008C YER008C SEC3 het Verified 0.253672886431629 0.399874141252371 no 426 0.38976632685799 1289 0.002 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress exocytosis|Golgi to plasma membrane transport|endoplasmic reticulum inheritance|exocyst localization|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis Rho GTPase binding|phosphatidylinositol-4,5-bisphosphate binding incipient cellular bud site|cellular bud neck|mating projection tip|exocyst|cellular bud tip YJL111W YJL111W CCT7 het Verified 0.251402730791552 0.400751378313853 no 427 0.442791694258172 1461 0.001 FF Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YCL017C YCL017C NFS1 het Verified 0.250769610529344 0.400996119118518 no 428 0.405656296884564 1335 0.000 FT KETONE METABOLISM|RNA PROCESSING|AMINO ACID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|COFACTOR METABOLISM NUCLEUS|MITOCHONDRION Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria cellular iron ion homeostasis|iron-sulfur cluster assembly|mitochondrial tRNA thio-modification|tRNA thio-modification|tRNA wobble uridine modification cysteine desulfurase activity mitochondrion|nucleus YPL020C YPL020C ULP1 het Verified 0.250560700701642 0.401076884438677 no 429 0.417393092999461 1386 0.004 FT CELL CYCLE|PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions G2/M transition of mitotic cell cycle|protein desumoylation cysteine-type peptidase activity|SUMO-specific protease activity nuclear envelope|nucleolus|nuclear pore YKL195W YKL195W MIA40 het Verified 0.246923299774799 0.402483791967586 no 430 0.423640939237792 1425 0.005 FF|FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p protein import into mitochondrial intermembrane space molecular_function mitochondrion|mitochondrial inner membrane|mitochondrial outer membrane translocase complex|mitochondrial intermembrane space YGR147C YGR147C NAT2 het Verified 0.241466098774305 0.404596944228973 no 431 0.406202313456288 1338 0.001 FT MITOCHONDRION Protein with an apparent role in acetylation of N-terminal methionine residues N-terminal peptidyl-methionine acetylation molecular_function mitochondrion YDL150W YDL150W RPC53 het Verified 0.240088391039044 0.405130866830297 no 432 0.416947589986965 1386 0.002 FF|FT NUCLEUS RNA polymerase III subunit C53 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YER127W YER127W LCP5 het Verified 0.237742061599861 0.40604057918252 no 433 0.394458536057205 1321 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus rRNA modification|rRNA processing RNA binding small nuclear ribonucleoprotein complex|small nucleolar ribonucleoprotein complex YGL008C YGL008C PMA1 het Verified 0.237675468754202 0.406066405806249 no 434 0.393966964068371 1310 0.003 FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS MITOCHONDRION|PLASMA MEMBRANE Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] proton transport|regulation of pH|transmembrane transport hydrogen-exporting ATPase activity, phosphorylative mechanism mitochondrion|integral to membrane|membrane raft|plasma membrane YPR019W YPR019W MCM4 het Verified 0.235129624212619 0.40705406317736 no 435 0.390821482506735 1286 0.001 FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p S phase of mitotic cell cycle|DNA strand elongation involved in DNA replication|DNA unwinding involved in replication|double-strand break repair via break-induced replication|DNA replication initiation|pre-replicative complex assembly four-way junction helicase activity|DNA helicase activity|DNA replication origin binding|single-stranded DNA-dependent ATPase activity|single-stranded DNA binding|ATP-dependent 3'-5' DNA helicase activity replication fork protection complex|nuclear replication fork|DNA replication preinitiation complex|nucleus|pre-replicative complex|cytoplasm|MCM complex YNL161W YNL161W CBK1 het Verified 0.234451707382722 0.407317160163022 no 436 0.394091714717547 1293 0.002 FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS|SITE OF POLARIZED GROWTH Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress regulation of fungal-type cell wall organization|establishment or maintenance of cell polarity|protein phosphorylation|establishment or maintenance of actin cytoskeleton polarity|budding cell apical bud growth|Unknown protein serine/threonine kinase activity incipient cellular bud site|cellular bud|cellular bud neck|cellular bud tip|cytoplasm|cell cortex|nucleus|mating projection tip YLL035W YLL035W GRC3 het Verified 0.232881973555922 0.407926528325087 no 437 0.401218710905037 1339 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated termination of RNA polymerase I transcription|rRNA processing polynucleotide 5'-hydroxyl-kinase activity nucleolar chromatin YMR079W YMR079W SEC14 het Verified 0.232732590619394 0.407984530174906 no 438 0.41523143159384 1384 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Golgi vesicle budding|phosphatidylinositol metabolic process|Golgi to plasma membrane protein transport|Golgi to vacuole transport|ascospore formation|Unknown|phospholipid transport phosphatidylinositol transporter activity|phosphatidylcholine transporter activity cytosol|Golgi apparatus|Golgi membrane|cytoplasm YBR198C YBR198C TAF5 het Verified 0.229103813582177 0.409394114740319 no 439 0.426919526322923 1397 0.001 FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter ubiquitin binding|chromatin binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YDL207W YDL207W GLE1 het Verified 0.228377545342579 0.409676372224653 no 440 0.388895028396805 1312 0.001 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|TRANSLATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION NUCLEAR PORE Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export poly(A)+ mRNA export from nucleus|mRNA export from nucleus|regulation of translational initiation|regulation of translational termination translation initiation factor binding|inositol hexakisphosphate binding|phospholipid binding|enzyme activator activity mitochondrion|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore|cytoplasm YHR170W YHR170W NMD3 het Verified 0.227545310390898 0.409999870255838 no 441 0.436840463803329 1444 0.004 FF|FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS RIBOSOME Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex ribosomal large subunit export from nucleus ribosomal large subunit binding cytosol|cytosolic large ribosomal subunit YKL154W YKL154W SRP102 het Verified 0.223962488640219 0.411393245777456 no 442 0.40288005212464 1348 0.001 FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane protein targeting to ER|SRP-dependent cotranslational protein targeting to membrane signal recognition particle binding|GTP binding|GTPase activity integral to endoplasmic reticulum membrane|signal recognition particle receptor complex YJR072C YJR072C NPA3 het Verified 0.222710029241227 0.411880597594345 no 443 0.391971004688685 1302 0.000 FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress cellular protein complex localization|nucleocytoplasmic transport|mitotic sister chromatid cohesion ATPase activity|GTPase activity cytoplasm YIL126W YIL126W STH1 het Verified 0.22230500231437 0.412038229123258 no 444 0.434356461125784 1444 0.003 FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS RSC COMPLEX ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p double-strand break repair|ATP-dependent chromatin remodeling|nucleosome positioning|chromosome segregation|chromatin remodeling at centromere|nucleosome disassembly|regulation of transcription, DNA-dependent|G2/M transition of mitotic cell cycle|meiosis|cytoskeleton organization|transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|DNA translocase activity|helicase activity|DNA-dependent ATPase activity RSC complex|nucleus YDR427W YDR427W RPN9 het Verified 0.219456644071035 0.413147174829247 no 445 0.40130184293018 1317 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress proteasome assembly|ubiquitin-dependent protein catabolic process structural molecule activity proteasome storage granule|proteasome regulatory particle, lid subcomplex YOR206W YOR206W NOC2 het Verified 0.215207658008519 0.414802710292235 no 446 0.428282308056588 1418 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors ribosomal subunit export from nucleus|ribosome assembly molecular_function mitochondrion|nucleus|Noc2p-Noc3p complex|Noc1p-Noc2p complex YOR176W YOR176W HEM15 het Verified 0.213266079725732 0.415559714693929 no 447 0.431995041232595 1432 0.003 FT COFACTOR METABOLISM MITOCHONDRION Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway heme biosynthetic process ferrochelatase activity mitochondrion|mitochondrial inner membrane YMR218C YMR218C TRS130 het Verified 0.210338367995019 0.416701795849672 no 448 0.411933193420939 1326 0.008 FT VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy regulation of Rab GTPase activity|early endosome to Golgi transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex|trans-Golgi network|early endosome YBR234C YBR234C ARC40 het Verified 0.202264458367938 0.419854991904318 no 449 0.421847144114886 1391 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches mitochondrion inheritance|actin nucleation|actin filament organization ubiquitin binding Arp2/3 protein complex YLR075W YLR075W RPL10 het Verified 0.199256608397881 0.421031010000196 no 450 0.432286249197826 1438 0.002 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects cytoplasmic translation|translational termination|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YLR145W YLR145W RMP1 het Verified 0.197695546623211 0.421641637114977 no 451 0.414711095078526 1378 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mRNA cleavage ribonuclease MRP activity nucleolus|nucleus|ribonuclease MRP complex|cytoplasm YDR404C YDR404C RPB7 het Verified 0.196386023450527 0.422154017465956 no 452 0.393309031955147 1300 0.002 FF|FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|TRANSLATION NUCLEUS RNA POL II, HOLOENZYME RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation transcription initiation from RNA polymerase II promoter|positive regulation of translational initiation|positive regulation of nuclear-transcribed mRNA poly(A) tail shortening|nuclear-transcribed mRNA catabolic process, exonucleolytic|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|translation initiation factor binding|single-stranded RNA binding|single-stranded DNA binding nucleus|DNA-directed RNA polymerase II, core complex|cytoplasmic mRNA processing body|cytoplasm YBL050W YBL050W SEC17 het Verified 0.194011212464249 0.423083551218525 no 453 0.413367110445326 1342 0.001 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP autophagy|SNARE complex disassembly|vesicle fusion with Golgi apparatus|vacuole fusion, non-autophagic ATPase activator activity|soluble NSF attachment protein activity SNARE complex|cytosol|extrinsic to membrane YER048W-A YER048W-A ISD11 het Verified 0.19315677088726 0.423418096481531 no 454 0.407362074158509 1348 0.001 FT COFACTOR METABOLISM MITOCHONDRION Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis iron-sulfur cluster assembly enzyme activator activity extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial matrix YOR075W YOR075W UFE1 het Verified 0.192344869665559 0.42373603679943 no 455 0.418276216786048 1388 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER retrograde vesicle-mediated transport, Golgi to ER|endoplasmic reticulum membrane fusion|vesicle fusion with endoplasmic reticulum SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum YIR015W YIR015W RPR2 het Verified 0.188811309877268 0.425120353640924 no 456 0.427862523697359 1406 0.003 FT RNA PROCESSING NUCLEUS Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress tRNA processing|intronic box C/D snoRNA processing ribonuclease P activity nucleolar ribonuclease P complex YGR115C_d YGR115C YGR115C het Dubious 0.186290164698785 0.426108609948699 no 457 0.44367053794696 1495 0.003 FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Unknown Unknown Unknown YDR187C_d YDR187C YDR187C het Dubious 0.183261871424974 0.427296274948127 no 458 0.43972287208513 1466 0.001 FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Unknown Unknown Unknown YPL228W YPL228W CET1 het Verified 0.18216664465091 0.427725974345717 no 459 0.426086314428202 1414 0.003 FT RNA PROCESSING NUCLEUS Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide 7-methylguanosine mRNA capping polynucleotide 5'-phosphatase activity mRNA cap methyltransferase complex|nucleus YLR323C YLR323C CWC24 het Verified 0.179263615738762 0.428865357236963 no 460 0.433963236955007 1439 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p generation of catalytic spliceosome for first transesterification step|snoRNA splicing molecular_function nucleus|U2-type spliceosomal complex YER168C YER168C CCA1 het Verified 0.178328937552485 0.429232327075749 no 461 0.422551751952044 1396 0.000 FT RNA PROCESSING NUCLEUS|MITOCHONDRION ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites tRNA 3'-terminal CCA addition tRNA adenylyltransferase activity mitochondrion|nucleus|mitochondrial matrix|cytoplasm YJL019W YJL019W MPS3 het Verified 0.1778540655506 0.429418792991355 no 462 0.413029758593201 1350 0.008 FT CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME|CYTOSKELETON Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p meiotic telomere clustering|synapsis|nuclear migration involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope|telomere tethering at nuclear periphery|mitotic sister chromatid cohesion molecular_function nuclear envelope|nuclear periphery|integral to membrane|nuclear chromosome, telomeric region|half bridge of spindle pole body YNL118C YNL118C DCP2 het Verified 0.17021737077084 0.432419595584564 no 463 0.435801791563649 1439 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA|deadenylation-independent decapping of nuclear-transcribed mRNA|stress granule assembly hydrolase activity|mRNA binding|m7G(5')pppN diphosphatase activity nucleus|cytoplasmic mRNA processing body|cytoplasm YLR186W YLR186W EMG1 het Verified 0.169866753859992 0.432557463834109 no 464 0.44577840263371 1464 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CYTOSKELETON Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal small subunit biogenesis|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA base methylation|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing rRNA (pseudouridine) methyltransferase activity small-subunit processome|nucleolus|nucleus|nuclear microtubule|cytoplasm|90S preribosome YER003C YER003C PMI40 het Verified 0.167549362343422 0.433468905442237 no 465 0.429575010623197 1441 0.001 FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM NUCLEUS Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation protein glycosylation|cell wall mannoprotein biosynthetic process|GDP-mannose biosynthetic process mannose-6-phosphate isomerase activity nucleus|cytoplasm YMR229C YMR229C RRP5 het Verified 0.166925751198046 0.43371423544653 no 466 0.404258544871773 1313 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) poly(U) RNA binding small-subunit processome|nucleolus|90S preribosome YGR048W YGR048W UFD1 het Verified 0.165593073213826 0.434238599403472 no 467 0.444906420917881 1467 0.001 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) retrograde protein transport, ER to cytosol|proteasomal ubiquitin-dependent protein catabolic process|ER-associated protein catabolic process|ER-associated misfolded protein catabolic process|Unknown|cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process ubiquitin binding|polyubiquitin binding nucleus|Cdc48p-Npl4p-Ufd1p AAA ATPase complex YGR277C YGR277C CAB4 het Verified 0.164442881441798 0.434691254252543 no 468 0.429907075876874 1431 0.001 FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved coenzyme A biosynthetic process pantetheine-phosphate adenylyltransferase activity nucleus|cytoplasm YGL030W YGL030W RPL30 het Verified 0.163820430434197 0.434936253900453 no 469 0.46479797782222 1555 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog cytoplasmic translation|negative regulation of mRNA splicing, via spliceosome|negative regulation of translation|rRNA processing structural constituent of ribosome|RNA binding|pre-mRNA 5'-splice site binding cytosolic large ribosomal subunit|cytoplasm YOL010W YOL010W RCL1 het Verified 0.163798412322068 0.434944920791133 no 470 0.412305582709965 1379 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA-3'-phosphate cyclase activity|endoribonuclease activity|enzyme activator activity nucleolus|90S preribosome YOR116C YOR116C RPO31 het Verified 0.162622837419166 0.435407702347707 no 471 0.41669370220459 1386 0.000 FF|FT NUCLEUS RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YGL011C YGL011C SCL1 het Verified 0.162441245235802 0.435479196563992 no 472 0.452141868216818 1508 0.001 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|nucleus|proteasome storage granule|nuclear outer membrane-endoplasmic reticulum membrane network YLR197W YLR197W NOP56 het Verified 0.161075102703996 0.436017124667589 no 473 0.461354187194784 1515 0.003 FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects rRNA modification|rRNA processing molecular_function box C/D snoRNP complex|small-subunit processome|nucleolus|nucleus|90S preribosome YKL172W YKL172W EBP2 het Verified 0.158322958840198 0.437101158880048 no 474 0.447728483467131 1489 0.004 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering nuclear division|rRNA processing molecular_function nuclear periphery|nucleolus|preribosome, large subunit precursor YDR235W YDR235W PRP42 het Verified 0.154070456215301 0.438777091315907 no 475 0.415038860218757 1374 0.002 FT RNA PROCESSING NUCLEUS U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats mRNA splicing, via spliceosome RNA binding U1 snRNP|U2-type prespliceosome YNL150W_d YNL150W YNL150W het Dubious 0.15233070646637 0.4394630536324 no 476 0.455842556083433 1520 0.002 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Unknown Unknown Unknown YIL063C YIL063C YRB2 het Verified 0.150412631742974 0.440219537977843 no 477 0.437703087751127 1444 0.000 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|GENE SILENCING ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential protein export from nucleus|regulation of chromatin silencing at telomere|ribosomal small subunit export from nucleus molecular_function integral to membrane|nucleus|nuclear pore YGR175C YGR175C ERG1 het Verified 0.15019006526226 0.4403073318815 no 478 0.421758097952038 1404 0.000 FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine ergosterol biosynthetic process drug binding|squalene monooxygenase activity endoplasmic reticulum|lipid particle YBR236C YBR236C ABD1 het Verified 0.150080927170619 0.440350383727438 no 479 0.44072264895967 1450 0.002 FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS RNA POL II, HOLOENZYME Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA 7-methylguanosine mRNA capping|transcription from RNA polymerase II promoter mRNA (guanine-N7-)-methyltransferase activity nucleus|DNA-directed RNA polymerase II, holoenzyme YDL043C YDL043C PRP11 het Verified 0.14843119485661 0.44100124140031 no 480 0.445583699004633 1488 0.006 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Subunit of the SF3a splicing factor complex, required for spliceosome assembly spliceosomal complex assembly RNA binding U2 snRNP|U2-type prespliceosome YDR311W YDR311W TFB1 het Verified 0.142230930560003 0.443448800542748 no 481 0.45409215441677 1502 0.002 FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators phosphorylation of RNA polymerase II C-terminal domain|transcription from RNA polymerase I promoter|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity|phosphatidylinositol-3-phosphate binding|phosphatidylinositol-5-phosphate binding core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YBR167C YBR167C POP7 het Verified 0.140415002560776 0.444166052477326 no 482 0.430142108713119 1415 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YGR047C YGR047C TFC4 het Verified 0.138178430551436 0.445049700743469 no 483 0.447537588390195 1474 0.002 FT NUCLEUS One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex YML046W YML046W PRP39 het Verified 0.136587233759715 0.445678533952634 no 484 0.436281227562027 1450 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats mRNA 5'-splice site recognition pre-mRNA 5'-splice site binding commitment complex|U1 snRNP|U2-type prespliceosome YPR048W YPR048W TAH18 het Verified 0.135526193887799 0.446097927789626 no 485 0.443545865895518 1457 0.007 FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM MITOCHONDRION Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance oxidation-reduction process|iron-sulfur cluster assembly oxidoreductase activity, acting on NADH or NADPH mitochondrion YCL031C YCL031C RRP7 het Verified 0.128591334422741 0.448840511752838 no 486 0.458431743502451 1512 0.005 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress ribosomal small subunit assembly|rRNA processing molecular_function nucleolus|nucleus|UTP-C complex|CURI complex YOL026C YOL026C MIM1 het Verified 0.12383917286349 0.450721307534391 no 487 0.444618471660633 1486 0.000 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex mitochondrial outer membrane translocase complex assembly|protein import into mitochondrial outer membrane molecular_function mitochondrial outer membrane|integral to mitochondrial outer membrane YMR113W YMR113W FOL3 het Verified 0.123743506807986 0.450759181458502 no 488 0.441557253878811 1475 0.001 FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis folic acid-containing compound biosynthetic process dihydrofolate synthase activity cytoplasm YOR157C YOR157C PUP1 het Verified 0.12307950472859 0.451022070363879 no 489 0.465879533569203 1514 0.002 FF|FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity proteasome storage granule|nucleus|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YGR253C YGR253C PUP2 het Verified 0.121496233268569 0.451648999010034 no 490 0.466053884475671 1555 0.001 FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YOR278W YOR278W HEM4 het Verified 0.120721641209522 0.451955758576666 no 491 0.465516080757228 1555 0.000 FT COFACTOR METABOLISM Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria heme biosynthetic process uroporphyrinogen-III synthase activity cellular_component YIL115C YIL115C NUP159 het Verified 0.120690346461678 0.451968152753861 no 492 0.439002960350299 1436 0.003 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|protein export from nucleus|ribosomal large subunit export from nucleus|ncRNA export from nucleus|nuclear pore distribution|ribosomal small subunit export from nucleus nucleocytoplasmic transporter activity|adenyl-nucleotide exchange factor activity nuclear pore central transport channel|integral to membrane|nuclear pore cytoplasmic filaments|nuclear pore YGR195W YGR195W SKI6 het Verified 0.117970998951716 0.453045318436832 no 493 0.451130356156013 1496 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) nuclear mRNA surveillance|U5 snRNA 3'-end processing|nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent CUT catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|U4 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|rRNA catabolic process|polyadenylation-dependent snoRNA 3'-end processing|U1 snRNA 3'-end processing|nuclear-transcribed mRNA catabolic process, non-stop decay|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YBR002C YBR002C RER2 het Verified 0.116700578005719 0.453548666112237 no 494 0.477995215365735 1781 0.001 FF|FT VESICLE-MEDIATED TRANSPORT|LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting dolichol biosynthetic process|protein glycosylation|ER to Golgi vesicle-mediated transport dehydrodolichyl diphosphate synthase activity|prenyltransferase activity endoplasmic reticulum YLR347C YLR347C KAP95 het Verified 0.115547677210567 0.454005516282252 no 495 0.456320232627488 1507 0.005 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p protein targeting to membrane|regulation of protein desumoylation|protein import into nucleus|phosphatidylcholine biosynthetic process|nuclear pore complex assembly protein transporter activity|Ran guanyl-nucleotide exchange factor activity integral to membrane|nuclear exosome (RNase complex)|nuclear pore|cytoplasm YJL031C YJL031C BET4 het Verified 0.110681907746376 0.455934296672789 no 496 0.437709082583637 1445 0.003 FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN PRENYLATION Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport Rab geranylgeranyltransferase activity Rab-protein geranylgeranyltransferase complex YKR038C YKR038C KAE1 het Verified 0.108085527088566 0.456963924301123 no 497 0.447933686515665 1475 0.002 FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|PROTEOLYSIS NUCLEUS|CHROMOSOME Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription chromosome organization|threonylcarbamoyladenosine metabolic process|telomere maintenance|positive regulation of transcription from RNA polymerase II promoter chromatin DNA binding nuclear chromatin|EKC/KEOPS complex|nucleus|cytoplasm YLR115W YLR115W CFT2 het Verified 0.106472219876912 0.457603847713501 no 498 0.467301481645813 1560 0.003 FT RNA PROCESSING NUCLEUS Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YDR325W YDR325W YCG1 het Verified 0.103529337962356 0.458771433133077 no 499 0.478323344361703 1606 0.005 FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation rDNA condensation|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation molecular_function nuclear condensin complex YHR164C YHR164C DNA2 het Verified 0.0986021494548746 0.460727081660923 no 500 0.45259146674621 1490 0.000 FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress DNA repair|lagging strand elongation|meiotic DNA double-strand break processing|DNA strand renaturation|replicative cell aging|telomere maintenance|DNA-dependent DNA replication ATP-dependent DNA helicase activity|single-stranded DNA specific endodeoxyribonuclease activity|5'-flap endonuclease activity nuclear chromosome, telomeric region|nucleus|cytoplasm YDL015C YDL015C TSC13 het Verified 0.0983532580476741 0.460825894665449 no 501 0.465702163102515 1544 0.001 FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress very long-chain fatty acid metabolic process oxidoreductase activity mitochondrion|integral to membrane|endoplasmic reticulum membrane YLR116W YLR116W MSL5 het Verified 0.0977787028927282 0.461054009481807 no 502 0.475896679591387 1566 0.005 FT RNA PROCESSING NUCLEUS Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA mRNA splicing, via spliceosome pre-mRNA branch point binding commitment complex YIL143C YIL143C SSL2 het Verified 0.0932670594803096 0.462845700355467 no 503 0.460077074123348 1527 0.001 FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION|RNA LOCALIZATION NUCLEUS RNA POL II, HOLOENZYME Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 poly(A)+ mRNA export from nucleus|promoter clearance from RNA polymerase II promoter|transcriptional open complex formation at RNA polymerase II promoter|nucleotide-excision repair, DNA incision|transcription from RNA polymerase II promoter|phosphorylation of RNA polymerase II C-terminal domain|regulation of transposition, RNA-mediated|regulation of mitotic recombination ATP-dependent DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YOL134C_d YOL134C YOL134C het Dubious 0.0920971481480262 0.463310427136216 no 504 0.463143043522476 1537 0.000 Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Unknown Unknown Unknown YDL208W YDL208W NHP2 het Verified 0.0920605932446352 0.463324948741216 no 505 0.462367731992002 1539 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing cleavage involved in rRNA processing|rRNA pseudouridine synthesis|snRNA pseudouridine synthesis snoRNA binding box H/ACA snoRNP complex YGL247W YGL247W BRR6 het Verified 0.090671971724147 0.463876621096348 no 506 0.469734885408913 1578 0.007 FT PROTEIN LOCALIZATION|LIPID METABOLISM|MEMBRANE ORGANIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism mRNA export from nucleus|protein export from nucleus|cellular lipid metabolic process molecular_function nuclear envelope YFL038C YFL038C YPT1 het Verified 0.0836335802189968 0.466673883650851 no 507 0.478010506184072 1586 0.006 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|SIGNALING|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Golgi vesicle budding|Golgi vesicle docking|early endosome to Golgi transport|macroautophagy|endocytic recycling|protein complex assembly|CVT pathway|retrograde vesicle-mediated transport, Golgi to ER|SNARE complex disassembly|ER to Golgi vesicle-mediated transport|COPII-coated vesicle budding SNARE binding|GTPase activity trans-Golgi network|mitochondrion|Golgi stack|endoplasmic reticulum membrane|Golgi membrane|pre-autophagosomal structure YDR160W YDR160W SSY1 het Verified 0.0771537236332595 0.469250627524121 no 508 0.459306983196877 1510 0.001 FT PLASMA MEMBRANE Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes response to amino acid stimulus molecular_function integral to membrane|plasma membrane YIL051C YIL051C MMF1 het Verified 0.0759274044480519 0.469738427094459 no 509 0.470389573759048 1578 0.001 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes isoleucine biosynthetic process|mitochondrial translation molecular_function mitochondrion|mitochondrial matrix YPR187W YPR187W RPO26 het Verified 0.0745499639216697 0.47028639313951 no 510 0.462251957415099 1524 0.008 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit tRNA transcription from RNA polymerase III promoter|transcription from RNA polymerase I promoter|transcription from RNA polymerase III promoter|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex|DNA-directed RNA polymerase I complex|DNA-directed RNA polymerase II, core complex YJL034W YJL034W KAR2 het Verified 0.066196500456467 0.473610691448323 no 511 0.478700453721801 1607 0.000 FT PROTEIN LOCALIZATION|PROTEOLYSIS ENDOPLASMIC RETICULUM ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p karyogamy involved in conjugation with cellular fusion|posttranslational protein targeting to membrane, translocation|ER-associated protein catabolic process|SRP-dependent cotranslational protein targeting to membrane, translocation|response to unfolded protein unfolded protein binding|ATPase activity endoplasmic reticulum|luminal surveillance complex YIR010W YIR010W DSN1 het Verified 0.0659334847828992 0.473715390786636 no 512 0.477403311017391 1585 0.003 FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YLR430W YLR430W SEN1 het Verified 0.0633541719849405 0.474742239678406 no 513 0.47779023000028 1600 0.001 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS tRNA processing|mRNA 3'-end processing|termination of RNA polymerase II transcription|mRNA polyadenylation|snoRNA 3'-end processing|rRNA processing|snRNA processing ATP-dependent 5'-3' DNA helicase activity|DNA-dependent ATPase activity|RNA-dependent ATPase activity|ATP-dependent 5'-3' RNA helicase activity|protein domain specific binding Nrd1 complex|nucleus YLR336C YLR336C SGD1 het Verified 0.0624793304315069 0.475090560851175 no 514 0.47306454732934 1580 0.002 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia ribosomal small subunit biogenesis|hyperosmotic response molecular_function nucleolus|nucleus|FAl1-SGD1 complex YPL255W YPL255W BBP1 het Verified 0.0600428345904829 0.476060759570297 no 515 0.475066367119211 1780 0.000 FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p microtubule nucleation|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton spindle pole body YLR223C YLR223C IFH1 het Verified 0.0600258461546282 0.476067524773337 no 516 0.456315796599682 1518 0.000 FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|GENE SILENCING NUCLEUS|CHROMOSOME Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|chromatin silencing at telomere transcription coactivator activity nuclear chromatin|nucleolus|nucleus|CURI complex YLR045C YLR045C STU2 het Verified 0.0596892614267887 0.47620156231302 no 517 0.471771390052686 1568 0.003 FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p microtubule nucleation|mitotic spindle organization in nucleus microtubule binding|structural constituent of cytoskeleton cell cortex|spindle microtubule|condensed nuclear chromosome kinetochore|spindle pole body|kinetochore YLL036C YLL036C PRP19 het Verified 0.0569113609794027 0.477307902114839 no 518 0.484414778942572 1623 0.007 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain generation of catalytic spliceosome for first transesterification step ubiquitin-protein ligase activity|first spliceosomal transesterification activity mitochondrion|nucleus|Prp19 complex|cytoplasm|U2-type catalytic step 1 spliceosome YBR193C YBR193C MED8 het Verified 0.049749512977381 0.480160999815305 no 519 0.479358839936038 1599 0.003 FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter TBP-class protein binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II transcription corepressor activity|structural molecule activity core mediator complex YBR254C YBR254C TRS20 het Verified 0.0470192800686755 0.481248930624717 no 520 0.48203493851723 1615 0.002 FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM|GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder ER to Golgi vesicle-mediated transport molecular_function TRAPP complex YOR294W YOR294W RRS1 het Verified 0.0453174972489024 0.481927120488872 no 521 0.485356674142298 1622 0.000 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus molecular_function nucleoplasm|nuclear periphery|nucleolus|preribosome, large subunit precursor YFR037C YFR037C RSC8 het Verified 0.0452231937751812 0.481964703600483 no 522 0.470818971666605 1550 0.000 FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters ATP-dependent chromatin remodeling|double-strand break repair via nonhomologous end joining|nucleosome disassembly|regulation of transcription, DNA-dependent|transcription elongation from RNA polymerase II promoter|G1/S transition of mitotic cell cycle DNA translocase activity|DNA binding RSC complex|nucleus YNL189W YNL189W SRP1 het Verified 0.0447678773400299 0.482146164789853 no 523 0.484163704949036 1619 0.004 FT PROTEIN LOCALIZATION NUCLEUS Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation protein targeting to membrane|protein import into nucleus protein transporter activity integral to membrane|nuclear exosome (RNase complex)|nucleus|cytoplasm YJL009W_d YJL009W YJL009W het Dubious 0.0412604089972264 0.483544147609181 no 524 0.479954123470837 1765 0.000 Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Unknown Unknown Unknown YER146W YER146W LSM5 het Verified 0.0407647763243921 0.483741710212629 no 525 0.482450430271835 1614 0.001 FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|U4/U6 x U5 tri-snRNP complex YLR272C YLR272C YCS4 het Verified 0.0402089975079024 0.483963252229291 no 526 0.466463138602865 1552 0.001 FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation maintenance of rDNA|meiotic chromosome condensation|mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation|chromatin silencing at silent mating-type cassette molecular_function nucleolus|nuclear condensin complex YAL038W YAL038W CDC19 het Verified 0.0396187651676491 0.4841985333717 no 527 0.4565027176246 1517 0.000 FT KETONE METABOLISM|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication pyruvate metabolic process|glycolysis pyruvate kinase activity cytosol|plasma membrane YFL022C YFL022C FRS2 het Verified 0.0392097581905208 0.4843615768548 no 528 0.49502435991957 1655 0.002 FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar phenylalanyl-tRNA aminoacylation phenylalanine-tRNA ligase activity phenylalanine-tRNA ligase complex|cytoplasm YKL186C YKL186C MTR2 het Verified 0.0370953153172083 0.485204503647352 no 529 0.497585457619616 1688 0.003 FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA poly(A)+ mRNA export from nucleus|ribosomal large subunit export from nucleus protein binding integral to membrane|nuclear RNA export factor complex|nuclear pore YPR107C YPR107C YTH1 het Verified 0.0337523569000002 0.486537313981686 no 530 0.480260349446951 1591 0.001 FF|FT RNA PROCESSING NUCLEUS Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YDR081C YDR081C PDC2 het Verified 0.0328178147970385 0.486909935855322 no 531 0.496738630961389 1665 0.001 FT TRANSCRIPTION FROM RNA POL II|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions positive regulation of thiamine biosynthetic process|Unknown|positive regulation of transcription from RNA polymerase II promoter transcription regulatory region sequence-specific DNA binding nucleus YPR161C YPR161C SGV1 het Verified 0.0318314414632711 0.487303236336588 no 532 0.490049208154432 1626 0.001 FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins phosphorylation of RNA polymerase II C-terminal domain|protein phosphorylation|transcription, DNA-dependent|positive regulation of histone H3-K4 methylation RNA polymerase II carboxy-terminal domain kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|nucleus YLR459W YLR459W GAB1 het Verified 0.0308048964009587 0.487712567755688 no 533 0.470678269882248 1564 0.002 FT CELL CYCLE|LIPID METABOLISM|CELL DIVISION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|GPI-anchor transamidase complex YER157W YER157W COG3 het Verified 0.0284561049118977 0.488649188522197 no 534 0.496506962951546 1666 0.000 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|ER to Golgi vesicle-mediated transport|macroautophagy|retrograde transport, vesicle recycling within Golgi|intra-Golgi vesicle-mediated transport|CVT pathway protein transporter activity Golgi transport complex YLR321C YLR321C SFH1 het Verified 0.0280854482845547 0.488797000041502 no 535 0.490784965450394 1643 0.001 FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME RSC COMPLEX Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog ATP-dependent chromatin remodeling|double-strand break repair|chromosome segregation|regulation of DNA replication involved in S phase|chromatin remodeling at centromere|nucleosome disassembly|G2/M transition of mitotic cell cycle|transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YDL017W YDL017W CDC7 het Verified 0.0272043836669439 0.489148359670434 no 536 0.488957506854862 1634 0.004 FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|PROTEIN PHOSPHORYLATION|GENE SILENCING NUCLEUS DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p negative regulation of exit from mitosis|regulation of chromatin silencing at telomere|protein phosphorylation|double-strand break repair via break-induced replication|DNA replication initiation protein serine/threonine kinase activity nucleus|Dbf4-dependent protein kinase complex YBR135W YBR135W CKS1 het Verified 0.0232772992197089 0.490714539702881 no 537 0.499129482123305 1673 0.001 FT CELL CYCLE|CHROMATIN ORGANIZATION|CELL DIVISION NUCLEUS Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters positive regulation of transcription, DNA-dependent|regulation of cell cycle|transcription, DNA-dependent|chromatin remodeling ubiquitin binding|protein kinase activator activity|histone binding cyclin-dependent protein kinase holoenzyme complex|nucleus|cytoplasm YKL145W YKL145W RPT1 het Verified 0.0227446359753996 0.490926985336821 no 538 0.49308016859355 1651 0.003 FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of protein catabolic process|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YDR246W YDR246W TRS23 het Verified 0.0201151901715809 0.49197574129632 no 539 0.495862875639494 1663 0.004 FT VESICLE-MEDIATED TRANSPORT ENDOPLASMIC RETICULUM|GOLGI APPARATUS One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 chromosome organization|ER to Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex YER133W YER133W GLC7 het Verified 0.0158443418488518 0.493679286594453 no 540 0.496273931755572 1658 0.000 FF|FT PROTEIN LOCALIZATION|TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CELL DIVISION|CARBOHYDRATE METABOLISM NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON KINETOCHORE Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest replication fork processing|glycogen metabolic process|chromosome segregation|regulation of cell shape during vegetative growth phase|termination of RNA polymerase II transcription, poly(A)-coupled|protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint|rRNA processing|regulation of cell cycle|ascospore formation|protein localization to kinetochore|cell budding|DNA damage checkpoint|meiosis|termination of RNA polymerase II transcription, exosome-dependent|DNA replication checkpoint|response to heat|cellular ion homeostasis|histone dephosphorylation|regulation of carbohydrate metabolic process protein serine/threonine phosphatase activity protein phosphatase type 1 complex|mating projection base|condensed nuclear chromosome kinetochore|mRNA cleavage and polyadenylation specificity factor complex|nucleolus|spindle pole body|cellular bud neck YKL210W YKL210W UBA1 het Verified 0.015530681647612 0.493804403513433 no 541 0.497354582351916 1669 0.002 FT NUCLEUS Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress protein ubiquitination ubiquitin activating enzyme activity nucleus|cytoplasm YGR002C YGR002C SWC4 het Verified 0.0131612054303867 0.494749590270198 no 542 0.495233209901556 1702 0.000 FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME HISTONE ACETYLTRANSFERASE Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex DNA repair|histone acetylation|chromosome organization|chromatin modification|chromatin remodeling|histone exchange|establishment of mitotic sister chromatid cohesion DNA binding Swr1 complex|histone acetyltransferase complex|nucleus|NuA4 histone acetyltransferase complex YPR088C YPR088C SRP54 het Verified 0.0128331554269723 0.494880452232553 no 543 0.482974973701351 1743 0.003 FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|SRP-dependent cotranslational protein targeting to membrane|protein targeting to ER 7S RNA binding|GTP binding|signal sequence binding signal recognition particle, endoplasmic reticulum targeting YGR191W YGR191W HIP1 het Verified 0.00975390605380945 0.496108816176521 no 544 0.487567303628015 1633 0.002 FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE High-affinity histidine permease, also involved in the transport of manganese ions manganese ion transport|histidine transport high affinity L-histidine transmembrane transporter activity integral to membrane|plasma membrane YOR326W YOR326W MYO2 het Verified 0.00727009218239796 0.497099678395097 no 545 0.483925874445258 1606 0.004 FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|PEROXISOME ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication vacuole inheritance|establishment of mitotic spindle orientation|peroxisome inheritance|budding cell apical bud growth|unidimensional cell growth|mitochondrion inheritance|membrane addition at site of cytokinesis|vesicle transport along actin filament|vesicle-mediated transport|Golgi inheritance microfilament motor activity|actin filament binding|calmodulin binding fungal-type vacuole membrane|incipient cellular bud site|filamentous actin|myosin V complex|actin filament bundle|cellular bud neck|cellular bud tip|vesicle|mating projection tip YLR310C YLR310C CDC25 het Verified 0.00332416270052253 0.498673853394161 no 546 0.492961159910935 1638 0.002 FT CELL CYCLE|SIGNALING|CELL DIVISION NUCLEUS|PLASMA MEMBRANE Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 regulation of cell cycle|replicative cell aging|Ras protein signal transduction|traversing start control point of mitotic cell cycle Ras guanyl-nucleotide exchange factor activity cytosol|nucleus|plasma membrane|cytoplasm YJR022W YJR022W LSM8 het Verified 0.00120483585929905 0.499519340151072 no 547 0.486210789785843 1629 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|nucleus|U4/U6 x U5 tri-snRNP complex YGR113W YGR113W DAM1 het Verified -0.00120483585929905 0.499519340151072 no 548 0.467713458035479 1549 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle midzone|DASH complex|condensed nuclear chromosome kinetochore|kinetochore microtubule|spindle YFR031C YFR031C SMC2 het Verified -0.00198875972969733 0.499206600181269 no 549 0.498548925405265 1671 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CHROMOSOME Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus mitotic chromosome condensation|mitotic sister chromatid segregation|tRNA gene clustering double-stranded DNA binding|ATPase activity|AT DNA binding|DNA secondary structure binding mitochondrion|nuclear condensin complex YLR066W YLR066W SPC3 het Verified -0.00282336511373551 0.498873641779561 no 550 0.491766423475325 1648 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex YMR146C YMR146C TIF34 het Verified -0.00744425482086462 0.497030199435438 no 551 0.435744534015896 1441 NA FT TRANSLATION eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation translation reinitiation|translational initiation translation initiation factor activity multi-eIF complex|eukaryotic translation initiation factor 3 complex YKR008W YKR008W RSC4 het Verified -0.00922108478175268 0.496321371540785 no 552 0.496979103366277 1660 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|transcription elongation from RNA polymerase II promoter histone acetyl-lysine binding|DNA translocase activity RSC complex YOL144W YOL144W NOP8 het Verified -0.0121326236994007 0.495159902178583 no 553 0.486327679905529 1737 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein required for 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus YDL105W YDL105W NSE4 het Verified -0.0126724785206691 0.494944547831608 no 554 0.481122115132764 1753 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair molecular_function Smc5-Smc6 complex|nucleus YIL021W YIL021W RPB3 het Verified -0.0131177427373236 0.494766927879383 no 555 0.49316018188303 1712 NA FF|FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity nucleoplasm|DNA-directed RNA polymerase II, core complex YNL102W YNL102W POL1 het Verified -0.0216712773571134 0.491355087869467 no 556 0.476230775704238 1770 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|MITOCHONDRION|CHROMOSOME Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis RNA-dependent DNA replication|lagging strand elongation|DNA synthesis involved in DNA repair|DNA replication|DNA replication initiation|premeiotic DNA replication DNA-directed DNA polymerase activity mitochondrion|alpha DNA polymerase:primase complex|replication fork YKL165C YKL165C MCD4 het Verified -0.0230201306684743 0.490817107626016 no 557 0.484744994473965 1745 NA FF|FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes GPI anchor biosynthetic process|ATP transport mannose-ethanolamine phosphotransferase activity integral to membrane|fungal-type cell wall|endoplasmic reticulum|fungal-type vacuole YIL083C YIL083C CAB2 het Verified -0.0249424980258023 0.490050414622327 no 558 0.488174513159446 1742 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) coenzyme A biosynthetic process phosphopantothenate--cysteine ligase activity nucleus|cytoplasm YHR042W YHR042W NCP1 het Verified -0.0251356290484772 0.489973390640903 no 559 0.491698952290579 1642 NA FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|PLASMA MEMBRANE NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p ergosterol biosynthetic process electron carrier activity mitochondrial outer membrane|mitochondrion YGL055W YGL055W OLE1 het Verified -0.0261891918074133 0.489553218307715 no 560 0.473356902270523 1775 NA FF|FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria unsaturated fatty acid biosynthetic process electron carrier activity|stearoyl-CoA 9-desaturase activity integral to endoplasmic reticulum membrane|integral to membrane YDR397C YDR397C NCB2 het Verified -0.0271148036959156 0.489184083730101 no 561 0.4892369180466 1731 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter transcription coactivator activity|transcription corepressor activity nucleus|negative cofactor 2 complex YDR145W YDR145W TAF12 het Verified -0.0272039217911331 0.489148543864052 no 562 0.483894463177271 1753 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter TBP-class protein binding|chromatin binding|RNA polymerase II activating transcription factor binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YOL146W YOL146W PSF3 het Verified -0.0280065733969815 0.488828454195992 no 563 0.487475886563617 1728 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function replication fork protection complex|GINS complex|DNA replication preinitiation complex YEL026W YEL026W SNU13 het Verified -0.0293698561497416 0.488284806868483 no 564 0.485799896449976 1742 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|mRNA splicing, via spliceosome RNA binding box C/D snoRNP complex|small-subunit processome|nucleolus|U4/U6 x U5 tri-snRNP complex YLL031C YLL031C GPI13 het Verified -0.0377329063979309 0.484950319579644 no 565 0.489741220355275 1720 NA FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity|transferase activity, transferring phosphorus-containing groups integral to membrane|endoplasmic reticulum YML125C YML125C PGA3 het Verified -0.0448178570375246 0.482126245768161 no 566 0.47481141790307 1779 NA FT PROTEIN LOCALIZATION|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication protein transport|chronological cell aging|replicative cell aging molecular_function endoplasmic reticulum|plasma membrane YJL202C_d YJL202C YJL202C het Dubious -0.0472016305424509 0.481176263993714 no 567 0.478076190223907 1772 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Unknown Unknown Unknown YGL111W YGL111W NSA1 het Verified -0.0497473680626461 0.48016185445425 no 568 0.473484937413746 1773 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis ribosomal large subunit biogenesis molecular_function nucleolus|nucleus|preribosome, large subunit precursor YDL084W YDL084W SUB2 het Verified -0.0520932921954223 0.47922717888175 no 569 0.490380644922337 1719 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress mRNA 3'-end processing|mRNA export from nucleus|transcription-coupled nucleotide-excision repair|mRNA splicing, via spliceosome|transcription elongation from RNA polymerase II promoter|chromatin silencing at telomere ATP-dependent RNA helicase activity chromosome, telomeric region|nucleus|transcription export complex|spliceosomal complex YPL204W YPL204W HRR25 het Verified -0.0547518979060836 0.47816806139388 no 570 0.482053647700273 1736 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CHROMOSOME|CYTOSKELETON KINETOCHORE Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) ribosomal small subunit biogenesis|DNA repair|mitosis|ER to Golgi vesicle-mediated transport|protein phosphorylation|attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation|tRNA wobble uridine modification protein serine/threonine kinase activity|protein kinase activity chromosome, centromeric region|plasma membrane|nucleus|spindle pole body|monopolin complex|cellular bud neck|cellular bud tip YLL050C YLL050C COF1 het Verified -0.0556570688662569 0.477807500269286 no 571 0.468834805558716 1816 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes actin filament depolymerization|actin filament severing actin filament binding plasma membrane|actin cortical patch YKL192C YKL192C ACP1 het Verified -0.0558972815660338 0.477711818220996 no 572 0.468639383990651 1815 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p lipoate biosynthetic process ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process mitochondrion YDL195W YDL195W SEC31 het Verified -0.078682893280036 0.468642426237257 no 573 0.472996693382761 1795 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance COPII-coated vesicle budding structural molecule activity COPII vesicle coat|mating projection tip YJL091C YJL091C GWT1 het Verified -0.0833493152443237 0.466786894391606 no 574 0.454534599104788 1844 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors GPI anchor biosynthetic process glucosaminyl-phosphotidylinositol O-acyltransferase activity integral to membrane|nuclear outer membrane-endoplasmic reticulum membrane network YDR339C YDR339C FCF1 het Verified -0.0838365289744862 0.46659320215959 no 575 0.469633151884828 1774 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YER093C YER093C TSC11 het Verified -0.0865683343935838 0.46550731843605 no 576 0.471068572952546 1805 NA FT LIPID METABOLISM|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain fungal-type cell wall organization|TOR signaling cascade|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|sphingolipid biosynthetic process protein binding TORC2 complex YMR301C YMR301C ATM1 het Verified -0.0882467601637087 0.464840276509618 no 577 0.468891560778466 1816 NA FT ION HOMEOSTASIS|NUCLEOTIDE METABOLISM|ION HOMEOSTASIS MITOCHONDRION Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol cellular iron ion homeostasis ATPase activity, coupled to transmembrane movement of substances|ATPase activity mitochondrion|integral to membrane|mitochondrial inner membrane YCL004W YCL004W PGS1 het Verified -0.0904413960688824 0.463968231078306 no 578 0.465616444951944 1801 NA FT LIPID METABOLISM MITOCHONDRION Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis cardiolipin biosynthetic process CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity mitochondrion YPL012W YPL012W RRP12 het Verified -0.0933212062525368 0.462824192721664 no 579 0.459347091388829 1847 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RIBOSOME Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosome biogenesis molecular_function ribosome|nucleus|90S preribosome YPR041W YPR041W TIF5 het Verified -0.0972882558118625 0.461248741130866 no 580 0.460267764320594 1839 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 mature ribosome assembly|negative regulation of translational initiation|regulation of translational initiation translation initiation factor binding|GDP-dissociation inhibitor activity|GTPase activator activity|translation initiation factor activity cytosolic small ribosomal subunit|multi-eIF complex YLR175W YLR175W CBF5 het Verified -0.0990915566443689 0.460532787957758 no 581 0.45983462122679 1836 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION NUCLEUS|CHROMOSOME|CYTOSKELETON Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita box H/ACA snoRNA 3'-end processing|rRNA pseudouridine synthesis|rRNA processing|snRNA pseudouridine synthesis pseudouridine synthase activity box H/ACA snoRNP complex|nucleolus|90S preribosome YOL123W YOL123W HRP1 het Verified -0.100191039990541 0.460096329636877 no 582 0.45444447573166 1857 NA FT RNA PROCESSING NUCLEUS Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences mRNA cleavage|mRNA polyadenylation mRNA binding|RNA binding mRNA cleavage factor complex|nucleus|cytoplasmic stress granule|cytoplasm YBR160W YBR160W CDC28 het Verified -0.107056186713841 0.457372202638683 no 583 0.451023709488238 1868 NA FT CELL CYCLE|LIPID METABOLISM|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION NUCLEUS|ENDOPLASMIC RETICULUM|SITE OF POLARIZED GROWTH|CYTOSKELETON Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress regulation of budding cell apical bud growth|negative regulation of meiotic cell cycle|positive regulation of triglyceride catabolic process|protein phosphorylation|regulation of filamentous growth|negative regulation of transcription, DNA-dependent|positive regulation of meiotic cell cycle|positive regulation of transcription, DNA-dependent|regulation of double-strand break repair via homologous recombination|vesicle-mediated transport|positive regulation of DNA replication involved in S phase|positive regulation of mitotic cell cycle|positive regulation of spindle pole body separation|chromatin remodeling|negative regulation of mitotic cell cycle histone binding|protein kinase activity|cyclin-dependent protein kinase activity cyclin-dependent protein kinase holoenzyme complex|ribosome|endoplasmic reticulum|nucleus|spindle pole body|cellular bud neck|astral microtubule|cytoplasm YDL058W YDL058W USO1 het Verified -0.108673098080753 0.456730890038796 no 584 0.436155908126124 1887 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS|CYTOSKELETON Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Golgi vesicle docking|ER to Golgi vesicle-mediated transport|SNARE complex assembly molecular_function integral to membrane|ER to Golgi transport vesicle membrane|Golgi membrane YPR105C YPR105C COG4 het Verified -0.109396164627551 0.456444137838164 no 585 0.447975548555424 1858 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT|RESPONSE TO NUTRIENT LEVELS|PEROXISOME ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments peroxisome degradation|intra-Golgi vesicle-mediated transport|retrograde transport, vesicle recycling within Golgi|macroautophagy|CVT pathway molecular_function Golgi transport complex YDR053W_d YDR053W YDR053W het Dubious -0.114232086668665 0.454526908449186 no 586 0.440716823505341 1890 NA Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Unknown Unknown Unknown YMR314W YMR314W PRE5 het Verified -0.114469781130695 0.454432700037618 no 587 0.464210696669745 1815 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YBR143C YBR143C SUP45 het Verified -0.114665237369 0.45435523434848 no 588 0.452433162649337 1854 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|CELL DIVISION Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p translational termination|cytokinesis translation release factor activity, codon specific|translation release factor activity cytosol|translation release factor complex YOR122C YOR122C PFY1 het Verified -0.118616505456125 0.452789594106266 no 589 0.455872889258361 1869 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE|CYTOSKELETON Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress positive regulation of formin-nucleated actin cable assembly|sequestering of actin monomers|intracellular transport actin monomer binding|phosphatidylinositol-4,5-bisphosphate binding|proline-rich region binding cytosol|extrinsic to plasma membrane YAL043C YAL043C PTA1 het Verified -0.120167111406171 0.452175385122232 no 590 0.433400741683142 1917 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII tRNA processing|termination of RNA polymerase II transcription, exosome-dependent|mRNA cleavage|termination of RNA polymerase II transcription, poly(A)-coupled|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YOR373W YOR373W NUD1 het Verified -0.124259963367537 0.4505547230813 no 591 0.450594431880159 1877 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON Component of the spindle pole body outer plaque; acts through the mitotic exit network to specify asymmetric spindle pole body inheritance meiotic chromosome segregation|spindle pole body separation|exit from mitosis|establishment of spindle localization structural constituent of cytoskeleton spindle pole body YNL306W YNL306W MRPS18 het Verified -0.128345236241944 0.448937883877903 no 592 0.427673589576509 1942 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial small ribosomal subunit YER172C YER172C BRR2 het Verified -0.128943776891208 0.448701068417713 no 593 0.424824751075655 1955 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) ATP-dependent RNA helicase activity U4/U6 x U5 tri-snRNP complex|U5 snRNP YOR319W YOR319W HSH49 het Verified -0.135943133417948 0.445933118215719 no 594 0.431907629802247 1953 NA FF|FT RNA PROCESSING NUCLEUS U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YDR091C YDR091C RLI1 het Verified -0.141398515024142 0.443777563661941 no 595 0.449774941601091 1892 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM NUCLEUS RIBOSOME Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species translational termination|ribosomal subunit export from nucleus|ribosomal large subunit biogenesis|translational initiation ATPase activity|iron ion binding cytosolic ribosome|nucleus|cytoplasm YDR190C YDR190C RVB1 het Verified -0.141914246226325 0.443573870674933 no 596 0.430232958952474 1948 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly regulation of transcription from RNA polymerase II promoter|box C/D snoRNP assembly|chromatin remodeling|histone exchange ATP-dependent 5'-3' DNA helicase activity|ATP-dependent 3'-5' DNA helicase activity ASTRA complex|R2TP complex|Swr1 complex|nucleus|Ino80 complex YPR177C_d YPR177C YPR177C het Dubious -0.142651235802295 0.443282815456379 no 597 0.451819309356313 1875 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Unknown Unknown Unknown YOL097C YOL097C WRS1 het Verified -0.147554972697333 0.441346996294022 no 598 0.437156671553042 1927 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA tryptophanyl-tRNA aminoacylation tryptophan-tRNA ligase activity cytoplasm YGR265W_d YGR265W YGR265W het Dubious -0.150712895427346 0.440101100178022 no 599 0.418039890639513 1969 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Unknown Unknown Unknown YOL038W YOL038W PRE6 het Verified -0.153551566682454 0.438981664045171 no 600 0.438726243476555 1908 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YMR268C YMR268C PRP24 het Verified -0.153552436944011 0.438981320930001 no 601 0.450562194965896 1857 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling assembly of spliceosomal tri-snRNP|spliceosomal complex assembly U6 snRNA binding|snRNA binding U6 snRNP YNL310C YNL310C ZIM17 het Verified -0.154498548993094 0.438608327609754 no 602 0.423505638709946 1963 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix protein folding|protein stabilization|protein import into mitochondrial matrix|response to unfolded protein|mitochondrion organization chaperone binding mitochondrion|mitochondrial matrix YBR091C YBR091C TIM12 het Verified -0.159701807450629 0.436557988519508 no 603 0.437754143492622 1912 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins protein import into mitochondrial inner membrane phospholipid binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YKL088W YKL088W CAB3 het Verified -0.161919958319549 0.435684442875025 no 604 0.446167206687199 1877 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC response to salt stress|coenzyme A biosynthetic process phosphopantothenoylcysteine decarboxylase activity|purine nucleotide binding phosphopantothenoylcysteine decarboxylase complex|cytoplasm YOR259C YOR259C RPT4 het Verified -0.1729021444303 0.431364173015276 no 605 0.4779245415408 1772 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization ER-associated protein catabolic process|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription elongation from RNA polymerase II promoter|nucleotide-excision repair|proteasome regulatory particle assembly ATPase activity|protein domain specific binding proteasome regulatory particle, base subcomplex|nucleus YPR113W YPR113W PIS1 het Verified -0.173121032700895 0.431278146429994 no 606 0.43883239544321 1906 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins phosphatidylinositol biosynthetic process CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity mitochondrial outer membrane|mitochondrion|integral to membrane|Golgi apparatus YDR082W YDR082W STN1 het Verified -0.176165632436247 0.430081909918269 no 607 0.422570581088125 1959 NA FT NUCLEUS|CHROMOSOME Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping telomere capping|negative regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YAR007C YAR007C RFA1 het Verified -0.184219445353521 0.426920652675958 no 608 0.424876506876714 1929 NA FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|DNA replication|double-strand break repair via homologous recombination|protein ubiquitination double-stranded DNA binding|single-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex|cytoplasm YDL209C YDL209C CWC2 het Verified -0.187094671574624 0.425793202931014 no 609 0.436220549597899 1913 NA FT CELL CYCLE|RNA PROCESSING NUCLEUS Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 mRNA cis splicing, via spliceosome|mRNA splicing, via spliceosome|spliceosomal snRNP assembly pre-mRNA binding|first spliceosomal transesterification activity|U6 snRNA binding|second spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome|U2-type catalytic step 2 spliceosome YDR365C YDR365C ESF1 het Verified -0.187870157820502 0.425489218385997 no 610 0.404045989931947 2009 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels rRNA processing RNA binding nucleolus|nucleus YER165W YER165W PAB1 het Verified -0.190413239589326 0.424492661887233 no 611 0.435028040303469 1906 NA FF|FT RNA PROCESSING|TRANSLATION|RNA LOCALIZATION NUCLEUS RIBOSOME Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G regulation of nuclear-transcribed mRNA poly(A) tail shortening|regulation of translational initiation poly(A) RNA binding ribosome|nucleus|cytoplasmic stress granule|cytoplasm YPR082C YPR082C DIB1 het Verified -0.191013973846713 0.424257322592142 no 612 0.413625110319303 1963 NA FT CELL CYCLE|RNA PROCESSING|CELL DIVISION NUCLEUS 17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex|U5 snRNP YPR035W YPR035W GLN1 het Verified -0.196304971125121 0.422185735348126 no 613 0.409570802647289 1986 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nitrogen compound metabolic process|glutamine biosynthetic process glutamate-ammonia ligase activity cytoplasm YGR140W YGR140W CBF2 het Verified -0.200516667698502 0.420538261879523 no 614 0.406691673755261 2009 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress chromosome segregation|septin ring assembly|mitotic spindle elongation DNA binding, bending|centromeric DNA binding spindle midzone|condensed nuclear chromosome kinetochore|CBF3 complex|condensed nuclear chromosome, centromeric region|spindle YPL075W YPL075W GCR1 het Verified -0.200829830353466 0.420415818427604 no 615 0.422353737469542 1976 NA FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|CARBOHYDRATE METABOLISM|RESPONSE TO NUTRIENT LEVELS|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|NUCLEUS Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p negative regulation of transcription from RNA polymerase II promoter by glucose|nucleosome organization|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter|regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from a mobile element promoter|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II distal enhancer sequence-specific DNA binding|RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity|RNA polymerase II core promoter proximal region sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription nuclear envelope YPL094C YPL094C SEC62 het Verified -0.204377515850773 0.419029249555772 no 616 0.394946527324633 2079 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation protein transporter activity integral to membrane|Sec62/Sec63 complex|endoplasmic reticulum membrane YJR067C YJR067C YAE1 het Verified -0.208272186470353 0.417508223718153 no 617 0.411439511767664 1980 NA FT NUCLEUS Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species biological_process molecular_function nucleus|cytoplasm YML127W YML127W RSC9 het Verified -0.208945743198107 0.417245297140915 no 618 0.47869774834698 1766 NA FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway G2/M transition of mitotic cell cycle|ATP-dependent chromatin remodeling|nucleosome disassembly|rRNA transcription|regulation of transcription from RNA polymerase II promoter|chromatin remodeling|transcription elongation from RNA polymerase II promoter|response to stress chromatin binding|DNA translocase activity RSC complex YDL028C YDL028C MPS1 het Verified -0.21476118484927 0.414976758373994 no 619 0.410069413774077 1994 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p protein phosphorylation|sister chromatid biorientation|spindle assembly|spindle pole body duplication associated with nuclear envelope|mitotic cell cycle spindle assembly checkpoint protein serine/threonine/tyrosine kinase activity|protein kinase activity condensed nuclear chromosome kinetochore|spindle pole body YKL045W YKL045W PRI2 het Verified -0.218515419915882 0.41351377262081 no 620 0.410855720012336 1988 NA FT RESPONSE TO DNA DAMAGE ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair lagging strand elongation|DNA replication, synthesis of RNA primer|DNA synthesis involved in DNA repair|DNA replication|DNA replication initiation single-stranded DNA binding|DNA primase activity nuclear envelope|alpha DNA polymerase:primase complex|nucleus YMR094W YMR094W CTF13 het Verified -0.22329500018478 0.411652959156955 no 621 0.390872530601187 2066 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME KINETOCHORE Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis kinetochore assembly|septin ring assembly DNA binding, bending|centromeric DNA binding condensed nuclear chromosome kinetochore|CBF3 complex YLR243W YLR243W GPN3 het Verified -0.224362050639896 0.411237798589714 no 622 0.413715290157839 1978 NA FT PROTEIN LOCALIZATION Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p biological_process signal sequence binding cellular_component YMR033W YMR033W ARP9 het Verified -0.224660841867192 0.411121564768602 no 623 0.448454121268261 1882 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation nucleosome positioning|ATP-dependent chromatin remodeling|nucleosome mobilization|chromatin organization|nucleosome disassembly|transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA translocase activity RSC complex|SWI/SNF complex|nucleus YGL225W YGL225W VRG4 het Verified -0.225186970945873 0.410916912425257 no 624 0.419562889703951 1962 NA FT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION|GOLGI APPARATUS Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi GDP-mannose transport GDP-mannose transmembrane transporter activity mitochondrion|integral to membrane|Golgi apparatus YIL144W YIL144W TID3 het Verified -0.22573476357688 0.410703859212778 no 625 0.408780564477133 2020 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering microtubule nucleation|chromosome segregation|protein localization to kinetochore structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YKL018W YKL018W SWD2 het Verified -0.225901385512858 0.410639060097256 no 626 0.436663789372308 1914 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|CHROMATIN ORGANIZATION NUCLEUS|CHROMOSOME Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination mRNA 3'-end processing|termination of RNA polymerase II transcription, exosome-dependent|histone H3-K4 methylation|termination of RNA polymerase II transcription, poly(A)-coupled|telomere maintenance|snoRNA 3'-end processing|histone H3-K79 methylation histone methyltransferase activity (H3-K4 specific) mRNA cleavage and polyadenylation specificity factor complex|Set1C/COMPASS complex YHR074W YHR074W QNS1 het Verified -0.227745467942158 0.409922061414521 no 627 0.410037195049647 2022 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM NUCLEUS Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide NAD biosynthetic process NAD+ synthase (glutamine-hydrolyzing) activity|hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides nucleus|cytoplasm YDR302W YDR302W GPI11 het Verified -0.229526926110321 0.409229697580119 no 628 0.413719536333289 2000 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog GPI anchor biosynthetic process mannose-ethanolamine phosphotransferase activity integral to membrane|endoplasmic reticulum|endoplasmic reticulum membrane YFR005C YFR005C SAD1 het Verified -0.231047190220781 0.408639070590652 no 629 0.402925629376204 2002 NA FT RNA PROCESSING|PROTEOLYSIS NUCLEUS Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle mRNA splicing, via spliceosome molecular_function nucleus YHR072W YHR072W ERG7 het Verified -0.234261380392564 0.407391032879476 no 630 0.397944975942662 2039 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis ergosterol biosynthetic process lanosterol synthase activity endoplasmic reticulum|lipid particle|plasma membrane YCR052W YCR052W RSC6 het Verified -0.235934072995959 0.406741914027764 no 631 0.462328505144949 1819 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p nucleosome positioning|ATP-dependent chromatin remodeling|G2/M transition of mitotic cell cycle|G1 phase of mitotic cell cycle|nucleosome disassembly|positive regulation of transcription elongation from RNA polymerase II promoter DNA translocase activity RSC complex YJR013W YJR013W GPI14 het Verified -0.237485343523436 0.406140144096401 no 632 0.408319348586676 2024 NA FT LIPID METABOLISM|CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M fungal-type cell wall organization|GPI anchor biosynthetic process mannosyltransferase activity integral to membrane|endoplasmic reticulum membrane YLR106C YLR106C MDN1 het Verified -0.237810905799414 0.406013879848753 no 633 0.397854960866324 2055 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM NUCLEUS|MITOCHONDRION Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus ribosomal large subunit assembly|rRNA processing ATPase activity nucleoplasm|mitochondrion|nucleus YLR339C_d YLR339C YLR339C het Dubious -0.240887348259729 0.404821214040372 no 634 0.386860488412914 2080 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Unknown Unknown Unknown YKL014C YKL014C URB1 het Verified -0.245317546576837 0.403105283286081 no 635 0.389983754681374 2068 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus YGL069C_d YGL069C YGL069C het Dubious -0.249482231446752 0.401493891857568 no 636 0.406370559884206 2008 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Unknown Unknown Unknown YOR060C YOR060C SLD7 het Verified -0.253724692567132 0.399854128123194 no 637 0.389409227055116 2073 NA FT CELL CYCLE ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|CYTOSKELETON Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria regulation of DNA-dependent DNA replication initiation molecular_function nuclear envelope|endoplasmic reticulum|DNA replication preinitiation complex|nucleus|spindle pole body|cytoplasm YHR190W YHR190W ERG9 het Verified -0.255995035768064 0.39897733525726 no 638 0.391841065053501 2067 NA FT LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway ergosterol biosynthetic process squalene synthase activity|farnesyl-diphosphate farnesyltransferase activity mitochondrial outer membrane|integral to membrane|endoplasmic reticulum YOR095C YOR095C RKI1 het Verified -0.259501215680815 0.397624272081355 no 639 0.384489021008555 2061 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|NUCLEOTIDE METABOLISM|COFACTOR METABOLISM|RESPONSE TO OXIDATIVE STRESS NUCLEUS Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis pyridoxine biosynthetic process|pentose-phosphate shunt ribose-5-phosphate isomerase activity nucleus|cytoplasm YKR004C YKR004C ECM9 het Verified -0.260474292819158 0.397248971231073 no 640 0.358469406657113 2180 NA FT CELL WALL ORG/BIOGENESIS Non-essential protein of unknown function fungal-type cell wall organization molecular_function cellular_component YML069W YML069W POB3 het Verified -0.263722574349567 0.395996849672423 no 641 0.391680727517353 2077 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress DNA replication-independent nucleosome organization|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|regulation of transcription by chromatin organization|DNA-dependent DNA replication chromatin binding|nucleosome binding|histone binding nuclear chromatin|FACT complex|replication fork protection complex|alpha DNA polymerase:primase complex YJL001W YJL001W PRE3 het Verified -0.263725947868595 0.395995549830518 no 642 0.406841000834173 1985 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YPR190C YPR190C RPC82 het Verified -0.265159617732336 0.395443251050183 no 643 0.415845520396773 1994 NA FT NUCLEUS RNA polymerase III subunit C82 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YLR008C YLR008C PAM18 het Verified -0.266719682008659 0.394842499337189 no 644 0.375318104580909 2107 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p protein import into mitochondrial matrix unfolded protein binding|ATPase activator activity mitochondrion|presequence translocase-associated import motor|mitochondrial inner membrane presequence translocase complex YBR252W YBR252W DUT1 het Verified -0.268937676270687 0.393988821607607 no 645 0.375638926799505 2123 NA FT NUCLEOTIDE METABOLISM NUCLEUS deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate pyrimidine deoxyribonucleoside triphosphate catabolic process|dUTP catabolic process|dITP catabolic process dITP diphosphatase activity|dUTP diphosphatase activity nucleus|cytoplasm YNL006W YNL006W LST8 het Verified -0.273137453043487 0.392373778772389 no 646 0.394682760119574 2077 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat transport|TOR signaling cascade|fungal-type cell wall organization|signal transduction|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|mitochondria-nucleus signaling pathway protein kinase activator activity|protein binding TORC1 complex|TORC2 complex|endosome membrane|extrinsic to internal side of plasma membrane|Golgi membrane YOR063W YOR063W RPL3 het Verified -0.27519645874546 0.391582653743278 no 647 0.397938445590493 2018 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome cytosolic large ribosomal subunit YDR468C YDR468C TLG1 het Verified -0.278505389657893 0.390312214148151 no 648 0.40372136120684 2015 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION GOLGI APPARATUS Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p endocytosis|vesicle fusion SNAP receptor activity trans-Golgi network|SNARE complex|endosome YJR141W_p YJR141W YJR141W het Uncharacterized -0.279755064307814 0.389832714537976 no 649 0.366656805721454 2174 NA Essential protein of unknown function biological_process molecular_function cellular_component YNL287W YNL287W SEC21 het Verified -0.280459830181219 0.389562370054657 no 650 0.397000823878225 2045 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo retrograde vesicle-mediated transport, Golgi to ER|ER to Golgi vesicle-mediated transport molecular_function endosome|COPI vesicle coat YOR361C YOR361C PRT1 het Verified -0.282573460981897 0.388751913293975 no 651 0.383186274881827 2105 NA FT TRANSLATION eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes translational initiation translation initiation factor activity eukaryotic translation initiation factor 3 complex|multi-eIF complex|cytoplasmic stress granule|cytoplasm YBR247C YBR247C ENP1 het Verified -0.282844853108876 0.388647884890905 no 652 0.382760112505948 2091 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing snoRNA binding preribosome, small subunit precursor|nucleolus|nucleus|cytoplasm|90S preribosome YJL090C YJL090C DPB11 het Verified -0.286320718897761 0.387316245352099 no 653 0.378404856475876 2079 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS|CHROMOSOME DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress lagging strand elongation|recombinational repair|nucleotide-excision repair|DNA replication initiation|mismatch repair|positive regulation of protein phosphorylation|mating type switching|DNA replication checkpoint|double-strand break repair via break-induced replication|mitotic cell cycle G2/M transition DNA damage checkpoint|leading strand elongation protein kinase activator activity|protein binding DNA replication preinitiation complex|replication fork|epsilon DNA polymerase complex YDR487C YDR487C RIB3 het Verified -0.286593277206256 0.387211881306488 no 654 0.373937923390195 2107 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION 3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration riboflavin biosynthetic process|aerobic respiration 3,4-dihydroxy-2-butanone-4-phosphate synthase activity cytosol|mitochondrial intermembrane space YPR175W YPR175W DPB2 het Verified -0.289677655759846 0.386031425930891 no 655 0.358429432840122 2167 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate DNA-dependent DNA replication double-stranded DNA binding|single-stranded DNA binding|DNA-directed DNA polymerase activity nucleus|epsilon DNA polymerase complex|cytoplasm YDL031W YDL031W DBP10 het Verified -0.296263748828645 0.383514334065322 no 656 0.379485586204492 2131 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus|preribosome, large subunit precursor YDR060W YDR060W MAK21 het Verified -0.299311117960766 0.38235133615681 no 657 0.382319211958987 2118 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein ribosomal large subunit assembly molecular_function Noc1p-Noc2p complex YJL156C YJL156C SSY5 het Verified -0.301293157149885 0.381595479222329 no 658 0.380274700098547 2117 NA FT PROTEOLYSIS PLASMA MEMBRANE Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p protein processing|response to amino acid stimulus serine-type endopeptidase activity extrinsic to plasma membrane YNR035C YNR035C ARC35 het Verified -0.301391276120555 0.381558072941405 no 659 0.39537394720791 2069 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin mitochondrion inheritance|receptor-mediated endocytosis|microtubule cytoskeleton organization|actin cytoskeleton organization structural molecule activity cytosol|Arp2/3 protein complex YNL232W YNL232W CSL4 het Verified -0.301805199606422 0.381400283443694 no 660 0.371117132008699 2151 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nucleus|nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex)|cytoplasm YIL104C YIL104C SHQ1 het Verified -0.302538662526442 0.381120732497367 no 661 0.391429659273661 2072 NA FT PROTEIN COMPLEX ORG/BIOGENESIS NUCLEUS Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones box H/ACA snoRNP assembly unfolded protein binding nucleoplasm YPR056W YPR056W TFB4 het Verified -0.30335694014591 0.380808928610196 no 662 0.389643920370494 2062 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YDR166C YDR166C SEC5 het Verified -0.307124295793398 0.379374382716597 no 663 0.3671581286004 2133 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Golgi to plasma membrane transport|endoplasmic reticulum inheritance|exocytosis|exocyst assembly|vesicle fusion|vesicle docking involved in exocytosis molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|exocyst|cellular bud tip YEL055C YEL055C POL5 het Verified -0.30843659304821 0.378875070536845 no 664 0.361989304465794 2159 NA FT NUCLEUS DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA rRNA transcription|DNA replication DNA-directed DNA polymerase activity nucleolus YMR235C YMR235C RNA1 het Verified -0.313122358266285 0.37709385158374 no 665 0.359674413923913 2175 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION|GENE SILENCING NUCLEUS GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport rRNA metabolic process|ribosomal subunit export from nucleus|tRNA metabolic process|rRNA export from nucleus|RNA import into nucleus|protein import into nucleus|chromatin silencing at telomere Ran GTPase activator activity cytosol|integral to membrane|nucleus YGR156W YGR156W PTI1 het Verified -0.313465536728514 0.37696349996959 no 666 0.369665478567259 2133 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 termination of RNA polymerase II transcription, exosome-dependent|mRNA cleavage|termination of RNA polymerase II transcription, poly(A)-coupled|mRNA polyadenylation|snoRNA 3'-end processing RNA binding mRNA cleavage and polyadenylation specificity factor complex|nucleus YCR054C YCR054C CTR86 het Verified -0.317740660831276 0.375340832704913 no 667 0.365798962256581 2152 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress biological_process molecular_function cytoplasm YPL131W YPL131W RPL5 het Verified -0.327337307793887 0.371706388881944 no 668 0.388912278680612 2092 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|RNA binding cytosolic large ribosomal subunit YJL104W YJL104W PAM16 het Verified -0.335361554548822 0.368676182718256 no 669 0.355701197864637 2178 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain protein import into mitochondrial matrix protein domain specific binding mitochondrion|presequence translocase-associated import motor|mitochondrial inner membrane presequence translocase complex YDR088C YDR088C SLU7 het Verified -0.337392919240899 0.367910360846086 no 670 0.31821853870243 2304 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity spliceosomal complex YDL164C YDL164C CDC9 het Verified -0.339125729039502 0.367257508360045 no 671 0.348340238006467 2194 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION NUCLEUS|MITOCHONDRION|CHROMOSOME DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination lagging strand elongation|base-excision repair|mitotic cell cycle|DNA ligation|DNA recombination|nucleotide-excision repair DNA ligase (ATP) activity mitochondrion|nucleus|replication fork YDR168W YDR168W CDC37 het Verified -0.343106553443929 0.365759150562009 no 672 0.361429974991376 2173 NA FT CELL CYCLE|SIGNALING|CELL DIVISION|CYTOSKELETON ORGANIZATION Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding regulation of stress-activated MAPK cascade|regulation of cell cycle|MAPK cascade involved in osmosensory signaling pathway|protein stabilization|spindle pole body duplication associated with nuclear envelope unfolded protein binding cytoplasm YKL182W YKL182W FAS1 het Verified -0.343210341744239 0.365720112549275 no 673 0.410409561231803 2002 NA FF|FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS MITOCHONDRION Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities fatty acid biosynthetic process palmitoyltransferase activity|enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity|fatty acid synthase activity|3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity|[acyl-carrier-protein] S-acetyltransferase activity|[acyl-carrier-protein] S-malonyltransferase activity cytosol|fatty acid synthase complex|mitochondrion|lipid particle|cytoplasm YLR317W_d YLR317W YLR317W het Dubious -0.346005729648863 0.364669204380576 no 674 0.34727963619276 2221 NA Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Unknown Unknown Unknown YDR398W YDR398W UTP5 het Verified -0.346071693041179 0.364644418107423 no 675 0.384782992313597 2078 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|t-UTP complex|rDNA heterochromatin|nucleolus YGR245C YGR245C SDA1 het Verified -0.346126944700804 0.364623657289099 no 676 0.360325077818919 2134 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|CYTOSKELETON ORGANIZATION NUCLEUS Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis ribosomal large subunit biogenesis|ribosomal large subunit export from nucleus|traversing start control point of mitotic cell cycle|actin cytoskeleton organization molecular_function nucleus YNL132W YNL132W KRE33 het Verified -0.346490417177547 0.364487092357969 no 677 0.346952884253284 2208 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance ribosomal small subunit biogenesis molecular_function nucleolus|90S preribosome YGL238W YGL238W CSE1 het Verified -0.34678745979074 0.364375499417109 no 678 0.37026261489164 2128 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION ENDOMEMBRANE SYSTEM|NUCLEUS Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation protein export from nucleus importin-alpha export receptor activity nuclear envelope|integral to membrane YOR329C YOR329C SCD5 het Verified -0.34830037177002 0.363807307336264 no 679 0.345270078205216 2198 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency protein secretion|endocytosis|actin filament organization|cortical actin cytoskeleton organization protein phosphatase 1 binding nucleus|actin cortical patch YGR090W YGR090W UTP22 het Verified -0.353022886398236 0.362035641453438 no 680 0.363312005114737 2165 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|UTP-C complex|90S preribosome|CURI complex YOR250C YOR250C CLP1 het Verified -0.353642768706877 0.361803309067459 no 681 0.373404787075759 2138 NA FT RNA PROCESSING NUCLEUS Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation mRNA 3'-end processing|mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage factor complex YKL021C YKL021C MAK11 het Verified -0.355996353837279 0.360921647883941 no 682 0.340916148900913 2192 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS ENDOMEMBRANE SYSTEM|NUCLEUS Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats ribosomal large subunit assembly|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit biogenesis|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function membrane YLR132C YLR132C USB1 het Verified -0.3580948799973 0.360136155258595 no 683 0.339689234725549 2227 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants U6 snRNA 3'-end processing phosphoric diester hydrolase activity mitochondrion|nucleus|cytoplasm YDL097C YDL097C RPN6 het Verified -0.361637115482861 0.358811612563005 no 684 0.385589054300084 2103 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress proteasome assembly|ubiquitin-dependent protein catabolic process structural molecule activity proteasome storage granule|proteasome regulatory particle, lid subcomplex YJR002W YJR002W MPP10 het Verified -0.367694007999457 0.356550704482652 no 685 0.386383824412888 2089 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|Mpp10 complex|nucleolus|nucleus|90S preribosome YJR093C YJR093C FIP1 het Verified -0.367987818742144 0.356441158562594 no 686 0.362465376057999 2129 NA FT RNA PROCESSING NUCLEUS Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p mRNA cleavage|mRNA polyadenylation RNA binding|protein binding, bridging mRNA cleavage and polyadenylation specificity factor complex YHR197W YHR197W RIX1 het Verified -0.369425005736194 0.355905481122575 no 687 0.354512106852415 2180 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene ribosomal large subunit assembly|ribosomal subunit export from nucleus|rRNA processing molecular_function nucleoplasm|nucleus YML130C YML130C ERO1 het Verified -0.36950757118955 0.355874715431278 no 688 0.330480865969778 2257 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds protein folding|protein thiol-disulfide exchange thiol oxidase activity endoplasmic reticulum YHR068W YHR068W DYS1 het Verified -0.370560805181895 0.355482339856869 no 689 0.353698262120154 2167 NA FT KETONE METABOLISM|AMINO ACID METABOLISM Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric peptidyl-lysine modification to hypusine transferase activity, transferring alkyl or aryl (other than methyl) groups cytoplasm YGR211W YGR211W ZPR1 het Verified -0.370804220857106 0.355391678675023 no 690 0.338265877908284 2235 NA FT CELL CYCLE|RESPONSE TO NUTRIENT LEVELS NUCLEUS Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress regulation of mitotic cell cycle|cellular response to starvation protein binding nucleus|cytoplasm YNL260C YNL260C LTO1 het Verified -0.371562043999297 0.355109476703958 no 691 0.354988305544901 2175 NA FT NUCLEUS Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species biological_process molecular_function nucleus|cytoplasm YKR022C YKR022C NTR2 het Verified -0.376290339304216 0.353350530285366 no 692 0.351167173114466 2190 NA FT RNA PROCESSING NUCLEUS|ENDOPLASMIC RETICULUM Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly spliceosomal complex disassembly|mRNA splicing, via spliceosome molecular_function endoplasmic reticulum|U2-type post-mRNA release spliceosomal complex|nucleus|spliceosomal complex|cytoplasm YML025C YML025C YML6 het Verified -0.379100389212874 0.352306658642036 no 693 0.334339273308351 2251 NA FT TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION RIBOSOME Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins mitochondrial translation structural constituent of ribosome mitochondrion|mitochondrial large ribosomal subunit YDR355C_d YDR355C YDR355C het Dubious -0.380260249030461 0.351876119915752 no 694 0.321151867910012 2271 NA Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Unknown Unknown Unknown YOR056C YOR056C NOB1 het Verified -0.380971288802251 0.351612276545371 no 695 0.331312787222962 2257 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|ENDOPLASMIC RETICULUM Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress proteasome assembly|ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA endoribonuclease activity|SSU rRNA binding preribosome, small subunit precursor|nucleus|cytoplasm YPR104C YPR104C FHL1 het Verified -0.382299865469192 0.351119477231441 no 696 0.379599133533656 2143 NA FT TRANSCRIPTION FROM RNA POL II|RIBOSOME BIOGENESIS NUCLEUS|CHROMOSOME Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter|positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter RNA polymerase II transcription corepressor binding|RNA polymerase II transcription coactivator binding|sequence-specific DNA binding|DNA binding nuclear chromatin|nucleolus|nucleus|CURI complex YOR098C YOR098C NUP1 het Verified -0.383129430112932 0.350811899688055 no 697 0.329316651076096 2268 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|nuclear pore nuclear basket|integral to membrane|nuclear pore YPL209C YPL209C IPL1 het Verified -0.383436251653542 0.350698164268144 no 698 0.34739028262233 2221 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest spindle checkpoint|attachment of spindle microtubules to kinetochore|chromosome segregation|mitotic spindle disassembly|protein phosphorylation|meiotic sister chromatid segregation|homologous chromosome segregation|regulation of cytokinesis|mitotic cell cycle G2/M transition decatenation checkpoint protein kinase activity spindle microtubule|spindle midzone|chromosome passenger complex|condensed nuclear chromosome kinetochore|kinetochore microtubule|spindle YDL003W YDL003W MCD1 het Verified -0.384258505318606 0.350393429745441 no 699 0.34890497757502 2204 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|MITOCHONDRION|CHROMOSOME Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase double-strand break repair|apoptotic process|response to DNA damage stimulus|mitotic chromosome condensation|establishment of mitotic sister chromatid cohesion chromatin binding condensed nuclear chromosome|mitochondrion|nucleus|nuclear mitotic cohesin complex YKL099C YKL099C UTP11 het Verified -0.384360311632989 0.35035570611368 no 700 0.346805668452126 2255 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus YGR082W YGR082W TOM20 het Verified -0.389094116843498 0.34860326267116 no 701 0.318917035631146 2288 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION|RNA LOCALIZATION MITOCHONDRION Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins tRNA import into mitochondrion|protein import into mitochondrial matrix|mitochondrial outer membrane translocase complex assembly protein channel activity|mitochondrion targeting sequence binding mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YMR260C YMR260C TIF11 het Verified -0.391851673536724 0.347583906025338 no 702 0.351390884136355 2189 NA FT TRANSLATION RIBOSOME Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 translational initiation double-stranded RNA binding|translation initiation factor activity ribosome YKL078W YKL078W DHR2 het Verified -0.391964309440867 0.347542292466039 no 703 0.341444842726811 2237 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosome biogenesis RNA helicase activity nucleolus YLR249W YLR249W YEF3 het Verified -0.39284459904286 0.347217131038826 no 704 0.332543769766879 2234 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION|NUCLEOTIDE METABOLISM RIBOSOME Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication translational elongation|translational termination ATPase activity|translation elongation factor activity cytosolic ribosome|ribosome YAL001C YAL001C TFC3 het Verified -0.393753689978323 0.346881448980387 no 705 0.396302826137282 2062 NA FF|FT PROTEIN LOCALIZATION NUCLEUS|MITOCHONDRION|CHROMOSOME Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding protein localization to chromatin|transcription from a RNA polymerase III hybrid type promoter|transcription from RNA polymerase III type 2 promoter|5S class rRNA transcription from RNA polymerase III type 1 promoter RNA polymerase III type 1 promoter sequence-specific DNA binding|RNA polymerase III type 2 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|RNA polymerase III type 2 promoter sequence-specific DNA binding|RNA polymerase III type 1 promoter sequence-specific DNA binding TFIIIB recruiting transcription factor activity|DNA binding, bending transcription factor TFIIIC complex|mitochondrion|nuclear condensin complex YDL087C YDL087C LUC7 het Verified -0.397408759586922 0.345533028514273 no 706 0.347841892023155 2208 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA mRNA splice site selection mRNA binding U1 snRNP|U2-type prespliceosome YKR079C YKR079C TRZ1 het Verified -0.397576467371414 0.345471204964027 no 707 0.323253036578038 2270 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 tRNA 3'-trailer cleavage, endonucleolytic 3'-tRNA processing endoribonuclease activity mitochondrion|nucleus|cytoplasm YMR270C YMR270C RRN9 het Verified -0.39833422101496 0.345191919188407 no 708 0.343823153751432 2207 NA FT NUCLEUS Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I transcription of nuclear large rRNA transcript from RNA polymerase I promoter TBP-class protein binding|RNA polymerase I upstream control element sequence-specific DNA binding transcription factor activity nucleolus|RNA polymerase I upstream activating factor complex YFL009W YFL009W CDC4 het Verified -0.400294450875287 0.344469827311739 no 709 0.339158888521119 2247 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p meiosis|G2/M transition of mitotic cell cycle|SCF-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|G1/S transition of mitotic cell cycle ubiquitin-protein ligase activity|ubiquitin binding nuclear SCF ubiquitin ligase complex|SCF ubiquitin ligase complex|nucleus|nuclear matrix YAR008W YAR008W SEN34 het Verified -0.401056814381106 0.344189147531094 no 710 0.329393094440981 2244 NA FT RNA PROCESSING ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YDR228C YDR228C PCF11 het Verified -0.407564348838619 0.341796771277309 no 711 0.374111822921879 2097 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping mRNA cleavage|termination of RNA polymerase II transcription|mRNA polyadenylation mRNA binding|RNA polymerase II core binding mRNA cleavage factor complex YNR038W YNR038W DBP6 het Verified -0.413184410383881 0.339735754542513 no 712 0.343399207611553 2242 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ATP-dependent RNA helicase activity nucleolus|preribosome, large subunit precursor YBR256C YBR256C RIB5 het Verified -0.41437446932105 0.339299941732041 no 713 0.353196452476893 2179 NA FT OXIDATION-REDUCTION PROCESS Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway riboflavin biosynthetic process riboflavin synthase activity cellular_component YJL195C_d YJL195C YJL195C het Dubious -0.415343151617006 0.338945358108987 no 714 0.35630123886493 2150 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Unknown Unknown Unknown YDR196C YDR196C CAB5 het Verified -0.416979534105812 0.33834668870781 no 715 0.313694445934844 2314 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies coenzyme A biosynthetic process dephospho-CoA kinase activity nuclear envelope|mitochondrion|endoplasmic reticulum YGL061C YGL061C DUO1 het Verified -0.419087677753682 0.337576027916298 no 716 0.297050193704906 2371 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|DASH complex|condensed nuclear chromosome kinetochore|spindle pole body|spindle YNR016C YNR016C ACC1 het Verified -0.420628273434845 0.337013272304369 no 717 0.369468325395449 2128 NA FF|FT PROTEIN LOCALIZATION|KETONE METABOLISM|LIPID METABOLISM|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; nuclear envelope organization|protein import into nucleus|fatty acid biosynthetic process biotin carboxylase activity|acetyl-CoA carboxylase activity mitochondrion|endoplasmic reticulum membrane YJL109C YJL109C UTP10 het Verified -0.426187637251441 0.334985562579471 no 718 0.331696249203032 2245 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION|CHROMOSOME Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|positive regulation of transcription from RNA polymerase I promoter|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding mitochondrion|90S preribosome|small-subunit processome|preribosome, small subunit precursor|integral to membrane|t-UTP complex|rDNA heterochromatin|nucleolus YKL024C YKL024C URA6 het Verified -0.429311667749816 0.333848213430821 no 719 0.327121897726308 2263 NA FT NUCLEOTIDE METABOLISM NUCLEUS Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) 'de novo' pyrimidine nucleobase biosynthetic process|nucleobase-containing compound metabolic process uridylate kinase activity|adenylate kinase activity nucleus|cytoplasm YGL233W YGL233W SEC15 het Verified -0.430950491008084 0.333252184778248 no 720 0.313840278507935 2357 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Golgi to plasma membrane transport|exocytosis|vesicle fusion|vesicle docking involved in exocytosis Rab GTPase binding incipient cellular bud site|cellular bud neck|mating projection tip|cellular bud tip|exocyst YDL147W YDL147W RPN5 het Verified -0.43133537732117 0.333112265265698 no 721 0.369326142810734 2158 NA FT PROTEOLYSIS NUCLEUS Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein cullin deneddylation|ubiquitin-dependent protein catabolic process molecular_function proteasome complex|signalosome|proteasome storage granule|proteasome regulatory particle, lid subcomplex YBR070C YBR070C ALG14 het Verified -0.433014317013999 0.33250218437264 no 722 0.334374329344622 2246 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases dolichol-linked oligosaccharide biosynthetic process protein anchor|N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity UDP-N-acetylglucosamine transferase complex|integral to endoplasmic reticulum membrane|nuclear outer membrane-endoplasmic reticulum membrane network|intrinsic to endoplasmic reticulum membrane YLR196W YLR196W PWP1 het Verified -0.438191380822381 0.330623778073402 no 723 0.301309525412606 2350 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily rRNA processing molecular_function nucleolus|nucleus|cytoplasm YNR053C YNR053C NOG2 het Verified -0.439581757259536 0.330120027879046 no 724 0.35289950171018 2179 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation ribosomal large subunit export from nucleus GTPase activity nucleoplasm|nucleolus|nucleus|preribosome, large subunit precursor YKL193C YKL193C SDS22 het Verified -0.443876972182171 0.328565767135305 no 725 0.311429799094521 2280 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function maintenance of protein location in nucleus|chromosome segregation protein serine/threonine phosphatase inhibitor activity|protein phosphatase type 1 activator activity protein phosphatase type 1 complex|nucleus|cytoplasm YGR172C YGR172C YIP1 het Verified -0.444747512032734 0.328251114811817 no 726 0.333852209445606 2239 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 vesicle-mediated transport|ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus molecular_function integral to membrane|ER to Golgi transport vesicle|integral to Golgi membrane|endoplasmic reticulum membrane YGR251W YGR251W NOP19 het Verified -0.446292698184516 0.32769291500574 no 727 0.334873799592591 2233 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|nucleolus|nucleus|90S preribosome YFR027W YFR027W ECO1 het Verified -0.447843083998455 0.327133223567798 no 728 0.304769719463691 2329 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress double-strand break repair|regulation of DNA replication|mitotic chromosome condensation|internal peptidyl-lysine acetylation|establishment of mitotic sister chromatid cohesion|DNA repair|chromosome organization|DNA replication|regulation of mitosis|telomere organization|tRNA gene clustering acetyltransferase activity|chromatin binding nuclear chromatin|nuclear replication fork YER013W YER013W PRP22 het Verified -0.447907116624016 0.327110116046964 no 729 0.301495006311634 2360 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes spliceosomal complex disassembly|generation of catalytic spliceosome for second transesterification step second spliceosomal transesterification activity|ATP-dependent RNA helicase activity U2-type post-spliceosomal complex|U2-type catalytic step 2 spliceosome YKL004W YKL004W AUR1 het Verified -0.447996765759527 0.327077765382752 no 730 0.332643573584263 2272 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance inositolphosphoceramide metabolic process|sphingolipid biosynthetic process inositol phosphoceramide synthase activity integral to membrane|Golgi apparatus|inositol phosphoceramide synthase complex YNL245C YNL245C CWC25 het Verified -0.454327826637422 0.324796445331569 no 731 0.313972922203694 2313 NA FT RNA PROCESSING NUCLEUS Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p mRNA splicing, via spliceosome molecular_function U2-type spliceosomal complex YLR230W_d YLR230W YLR230W het Dubious -0.455263581769143 0.324459811528278 no 732 0.331622716931812 2257 NA Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Unknown Unknown Unknown YNL152W YNL152W INN1 het Verified -0.458224122988214 0.323395715328304 no 733 0.338779887284001 2245 NA FT CELL CYCLE|CELL DIVISION SITE OF POLARIZED GROWTH|CYTOSKELETON Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis cytokinesis after mitosis|chromosome organization|barrier septum assembly involved in cell cycle cytokinesis|cytokinesis phospholipid binding cellular bud neck contractile ring|cytoplasm YMR211W YMR211W DML1 het Verified -0.459895307999241 0.32279568409764 no 734 0.359577587348902 2152 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family plasmid maintenance|mitochondrial genome maintenance molecular_function mitochondrion|cytoplasm YDL060W YDL060W TSR1 het Verified -0.460574732261566 0.322551871696632 no 735 0.308027586042432 2329 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress rRNA processing|ribosome biogenesis ribonucleoprotein complex binding nucleolus|cytoplasm|90S preribosome YBR136W YBR136W MEC1 het Verified -0.460612415714424 0.322538351165857 no 736 0.300675099247691 2373 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS|MITOCHONDRION Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres histone phosphorylation|telomere maintenance via recombination|telomere maintenance|nucleobase-containing compound metabolic process|reciprocal meiotic recombination|DNA recombination|DNA damage checkpoint|DNA replication|DNA damage induced protein phosphorylation protein kinase activity mitochondrion|nucleus YER021W YER021W RPN3 het Verified -0.461184167759972 0.322333239765278 no 737 0.356048264054172 2193 NA FT PROTEOLYSIS Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control ubiquitin-dependent protein catabolic process molecular_function proteasome regulatory particle, lid subcomplex YHR166C YHR166C CDC23 het Verified -0.463260921092427 0.321588676712065 no 738 0.309132432934275 2294 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS|CHROMOSOME UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity|cyclin binding anaphase-promoting complex YKR062W YKR062W TFA2 het Verified -0.466229735634156 0.320525533518095 no 739 0.326885939836267 2271 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIIE small subunit, involved in RNA polymerase II transcription initiation transcription from RNA polymerase II promoter single-stranded DNA binding|RNA polymerase II core binding|TFIIH-class transcription factor binding transcription factor TFIIE complex YGR075C YGR075C PRP38 het Verified -0.468231308340803 0.319809592590771 no 740 0.315181626642898 2313 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) molecular_function U4/U6 x U5 tri-snRNP complex YPR162C YPR162C ORC4 het Verified -0.470464228663565 0.319011692643351 no 741 0.321187818257407 2288 NA FT GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing pre-replicative complex assembly|DNA replication initiation|chromatin silencing at silent mating-type cassette DNA replication origin binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YGR095C YGR095C RRP46 het Verified -0.471611245246014 0.318602149307649 no 742 0.34574411052208 2237 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) nuclear polyadenylation-dependent tRNA catabolic process|nuclear polyadenylation-dependent rRNA catabolic process|nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay|nuclear polyadenylation-dependent mRNA catabolic process|nonfunctional rRNA decay|nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'|polyadenylation-dependent snoRNA 3'-end processing|exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|nuclear-transcribed mRNA catabolic process, non-stop decay|ncRNA 3'-end processing molecular_function nuclear exosome (RNase complex)|cytoplasmic exosome (RNase complex) YKL089W YKL089W MIF2 het Verified -0.471660990849017 0.318584392601947 no 743 0.330446872275114 2279 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 chromosome segregation|mitotic spindle organization in nucleus centromeric DNA binding condensed nuclear chromosome, centromeric region|condensed nuclear chromosome inner kinetochore YOR117W YOR117W RPT5 het Verified -0.472310657546293 0.31835253217052 no 744 0.355336031394934 2183 NA FF|FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YGL097W YGL097W SRM1 het Verified -0.474757642180873 0.317479863667727 no 745 0.314311745401222 2318 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|CELL DIVISION|RNA LOCALIZATION NUCLEUS|CHROMOSOME Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p ribosomal subunit export from nucleus|rRNA export from nucleus signal transducer activity nuclear chromatin|integral to membrane|nucleus YMR239C YMR239C RNT1 het Verified -0.476715076751346 0.316782512714535 no 746 0.308424223916858 2290 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION NUCLEUS Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes U5 snRNA 3'-end processing|mRNA cleavage|chromatin organization|termination of RNA polymerase II transcription, exosome-dependent|U4 snRNA 3'-end processing|U1 snRNA 3'-end processing|rRNA transcription|rRNA processing ribonuclease III activity nucleoplasm|nucleolus YFR052W YFR052W RPN12 het Verified -0.477190618421958 0.316613195500172 no 747 0.316561771780626 2297 NA FT PROTEOLYSIS Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress ubiquitin-dependent protein catabolic process molecular_function proteasome storage granule|proteasome regulatory particle, lid subcomplex YMR168C YMR168C CEP3 het Verified -0.480725983088751 0.315355630902614 no 748 0.308384465890983 2334 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME KINETOCHORE Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain septin ring assembly|mitotic cell cycle spindle assembly checkpoint sequence-specific DNA binding|DNA binding, bending|centromeric DNA binding condensed nuclear chromosome kinetochore|CBF3 complex YBR237W YBR237W PRP5 het Verified -0.481021230870273 0.31525070476071 no 749 0.292356551706748 2378 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA mRNA branch site recognition RNA-dependent ATPase activity spliceosomal complex YGR267C YGR267C FOL2 het Verified -0.483817940799678 0.314257540699223 no 750 0.301451600228743 2349 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM NUCLEUS GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway folic acid-containing compound biosynthetic process GTP cyclohydrolase I activity nucleus|cytoplasm YMR213W YMR213W CEF1 het Verified -0.487692161923381 0.312883952215893 no 751 0.282402457516638 2383 NA FT CELL CYCLE|RNA PROCESSING NUCLEUS Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p mRNA splicing, via spliceosome first spliceosomal transesterification activity Prp19 complex|U2-type catalytic step 1 spliceosome YPL266W YPL266W DIM1 het Verified -0.487982384722227 0.312781159164077 no 752 0.299846234547363 2388 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing rRNA modification|rRNA processing rRNA (adenine-N6,N6-)-dimethyltransferase activity nucleolus|90S preribosome YLR022C YLR022C SDO1 het Verified -0.489636851021844 0.312195447447695 no 753 0.306668358233475 2324 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes mature ribosome assembly molecular_function nucleus|preribosome, large subunit precursor|polysome|cytoplasm YBR060C YBR060C ORC2 het Verified -0.492758549026852 0.311091601216714 no 754 0.289771551181476 2401 NA FT CHROMATIN ORGANIZATION|GENE SILENCING NUCLEUS|CHROMOSOME Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p histone H3-K4 methylation|DNA replication initiation|pre-replicative complex assembly|chromatin silencing at silent mating-type cassette|chromatin silencing at telomere chromatin binding|DNA replication origin binding DNA replication preinitiation complex|pre-replicative complex|nuclear origin of replication recognition complex YBL026W YBL026W LSM2 het Verified -0.495019857448238 0.310293051757875 no 755 0.345666496302345 2208 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome RNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YDR232W YDR232W HEM1 het Verified -0.498572144864277 0.309040416327805 no 756 0.311917361907836 2332 NA FT COFACTOR METABOLISM MITOCHONDRION 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p heme biosynthetic process 5-aminolevulinate synthase activity mitochondrion|mitochondrial matrix YGR046W YGR046W TAM41 het Verified -0.500732226222512 0.308279794469381 no 757 0.31998847495468 2268 NA FT PROTEIN LOCALIZATION|LIPID METABOLISM|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition cardiolipin biosynthetic process|protein import into mitochondrial matrix molecular_function extrinsic to mitochondrial inner membrane|mitochondrion|mitochondrial matrix YOR004W YOR004W UTP23 het Verified -0.501380440409691 0.30805170136307 no 758 0.270478355070374 2468 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|mitochondrion|nucleolus YML085C YML085C TUB1 het Verified -0.502310103253795 0.307724701716975 no 759 0.356404607171373 2186 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION NUCLEUS|CYTOSKELETON Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|cytoplasmic microtubule|kinetochore microtubule|nuclear microtubule|spindle pole body|polar microtubule YGL122C YGL122C NAB2 het Verified -0.502759454068683 0.307566701759133 no 760 0.282296404906138 2424 NA FT RNA PROCESSING|RNA LOCALIZATION NUCLEUS Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress poly(A)+ mRNA export from nucleus|mRNA polyadenylation|regulation of mRNA stability poly(A) RNA binding nucleus|cytoplasm YJL033W YJL033W HCA4 het Verified -0.50280872838763 0.307549378179171 no 761 0.308005768381339 2321 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis rRNA processing RNA-dependent ATPase activity|ATP-dependent RNA helicase activity nucleolus YLR129W YLR129W DIP2 het Verified -0.503519935713816 0.307299383842613 no 762 0.293928724056722 2405 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YNL251C YNL251C NRD1 het Verified -0.505864465037243 0.306475899831781 no 763 0.294036041304281 2383 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites mRNA 3'-end processing|nuclear mRNA surveillance|termination of RNA polymerase II transcription, exosome-dependent|antisense RNA transcript catabolic process|tRNA 3'-end processing|snRNA 3'-end processing|snoRNA 3'-end processing|CUT catabolic process RNA binding|transcription regulatory region RNA binding|protein domain specific binding Nrd1 complex|nucleus YDL153C YDL153C SAS10 het Verified -0.50745230707167 0.305918746105853 no 764 0.300664040862361 2329 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|GENE SILENCING NUCLEUS Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function small-subunit processome|nucleolus|nucleus YNL247W YNL247W YNL247W het Verified -0.509464976745639 0.305213170760869 no 765 0.29073838320301 2410 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments cysteinyl-tRNA aminoacylation cysteine-tRNA ligase activity ribosome|cytoplasm YHR065C YHR065C RRP3 het Verified -0.509963655379531 0.305038462240889 no 766 0.258600702963857 2511 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) RNA-dependent ATPase activity|ATP-dependent RNA helicase activity nucleolus YDR353W YDR353W TRR1 het Verified -0.512807670018538 0.304042931992519 no 767 0.30753916499512 2335 NA FT OXIDATION-REDUCTION PROCESS|RESPONSE TO OXIDATIVE STRESS Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication cellular response to oxidative stress|cell redox homeostasis ferrous iron binding|thioredoxin-disulfide reductase activity cytosol YOL130W YOL130W ALR1 het Verified -0.514564475399157 0.303428697358325 no 768 0.299150330705123 2364 NA FT ION HOMEOSTASIS|ION HOMEOSTASIS PLASMA MEMBRANE Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions magnesium ion transport|cation transport inorganic cation transmembrane transporter activity integral to membrane|plasma membrane YBL105C YBL105C PKC1 het Verified -0.515119236097394 0.303234850736819 no 769 0.357338547010747 2183 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION|PEROXISOME ORGANIZATION NUCLEUS|SITE OF POLARIZED GROWTH|CYTOSKELETON Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) intracellular protein kinase cascade|regulation of nuclear-transcribed mRNA poly(A) tail shortening|fungal-type cell wall organization|signal transduction|actin filament organization|peroxisome degradation|protein phosphorylation|cytoplasmic mRNA processing body assembly protein kinase C activity site of polarized growth|cytoskeleton|nucleus|plasma membrane|cytoplasm YBL097W YBL097W BRN1 het Verified -0.517759600568074 0.30231300429464 no 770 0.305326973241185 2345 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission mitotic chromosome condensation|tRNA gene clustering|mitotic sister chromatid segregation molecular_function nucleus|SMC loading complex|nuclear condensin complex YLR424W YLR424W SPP382 het Verified -0.524193493842169 0.300071982837336 no 771 0.31222834945494 2318 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS|MITOCHONDRION Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation spliceosomal complex disassembly|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step RNA binding|ATP-dependent RNA helicase activity mitochondrion|U2-type post-mRNA release spliceosomal complex|nucleus|spliceosomal complex|cytoplasm YIL031W YIL031W ULP2 het Verified -0.524784306387237 0.29986657120314 no 772 0.312553123229086 2292 NA FT CELL CYCLE|PROTEOLYSIS NUCLEUS Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate plasmid maintenance|protein desumoylation|chromosome condensation|mitotic cell cycle spindle assembly checkpoint cysteine-type peptidase activity|SUMO-specific protease activity nucleus YLR378C YLR378C SEC61 het Verified -0.527442144387552 0.298943291726357 no 773 0.285686129431934 2457 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER posttranslational protein targeting to membrane, translocation|retrograde protein transport, ER to cytosol|ER-associated protein catabolic process|SRP-dependent cotranslational protein targeting to membrane, translocation|peptide transport peptide transporter activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity|signal sequence binding integral to membrane|Sec61 translocon complex YJL072C YJL072C PSF2 het Verified -0.532099624527445 0.297328498716825 no 774 0.307502601759525 2331 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|nuclear replication fork|nucleus|DNA replication preinitiation complex YGL098W YGL098W USE1 het Verified -0.534036647570768 0.296658089635113 no 775 0.272694522174885 2441 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p retrograde vesicle-mediated transport, Golgi to ER|vesicle fusion with endoplasmic reticulum SNAP receptor activity SNARE complex|integral to cytosolic side of endoplasmic reticulum membrane YOR244W YOR244W ESA1 het Verified -0.534654551161508 0.296444377213103 no 776 0.29209555087955 2380 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS HISTONE ACETYLTRANSFERASE Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy DNA repair|histone acetylation|regulation of cell cycle|regulation of transcription by chromatin organization|DNA-dependent transcription, elongation|chromatin silencing at rDNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter H4 histone acetyltransferase activity|histone acetyltransferase activity Piccolo NuA4 histone acetyltransferase complex|NuA4 histone acetyltransferase complex YOR262W YOR262W GPN2 het Verified -0.534973342395593 0.296334145466726 no 777 0.30302117528006 2361 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMOSOME SEGREGATION Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion establishment of mitotic sister chromatid cohesion molecular_function cytoplasm YDL030W YDL030W PRP9 het Verified -0.539848760836592 0.294650668331145 no 778 0.290646066696943 2390 NA FT RNA PROCESSING NUCLEUS Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex mRNA splicing, via spliceosome RNA binding U2 snRNP|U2-type prespliceosome YLL037W_d YLL037W YLL037W het Dubious -0.54004385973181 0.294583392769731 no 779 0.282737496415176 2439 NA Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Unknown Unknown Unknown YNL124W YNL124W NAF1 het Verified -0.540792407454645 0.294325338340032 no 780 0.282354769801694 2437 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p box H/ACA snoRNP assembly RNA binding nucleoplasm|nucleus YDR224C YDR224C HTB1 het Verified -0.541824282625279 0.293969780814049 no 781 0.302352313477056 2362 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation chromatin assembly or disassembly|negative regulation of transcription from RNA polymerase II promoter|postreplication repair DNA binding replication fork protection complex|nuclear nucleosome YDR243C YDR243C PRP28 het Verified -0.544369186963046 0.293093722563455 no 782 0.279527937336053 2413 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site cis assembly of pre-catalytic spliceosome ATP-dependent RNA helicase activity spliceosomal complex|U5 snRNP YKL019W YKL019W RAM2 het Verified -0.546739000141565 0.292279028165296 no 783 0.275900936793574 2433 NA FT PROTEIN PRENYLATION Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor protein farnesylation|protein geranylgeranylation|peptide pheromone maturation protein geranylgeranyltransferase activity|protein farnesyltransferase activity|CAAX-protein geranylgeranyltransferase activity CAAX-protein geranylgeranyltransferase complex|protein farnesyltransferase complex YMR117C YMR117C SPC24 het Verified -0.548783181186374 0.291577126890541 no 784 0.26597532929282 2498 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YKL152C YKL152C GPM1 het Verified -0.550409879207946 0.291019137079523 no 785 0.267998634386684 2491 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis gluconeogenesis|glycolysis phosphoglycerate mutase activity cytosol|mitochondrion YKL058W YKL058W TOA2 het Verified -0.550838986620002 0.290872027890469 no 786 0.296594060100988 2348 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly|TBP-class protein binding RNA polymerase II transcription factor activity transcription factor TFIIA complex YOR257W YOR257W CDC31 het Verified -0.551401432860447 0.290679259335586 no 787 0.339542884991397 2223 NA FF|FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|PROTEOLYSIS|CYTOSKELETON ORGANIZATION|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|CYTOSKELETON NUCLEAR PORE Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation microtubule nucleation|proteasomal ubiquitin-dependent protein catabolic process|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton integral to membrane|transcription export complex 2|nucleotide-excision repair factor 2 complex|half bridge of spindle pole body|nuclear pore YIL106W YIL106W MOB1 het Verified -0.551744962571463 0.290561549971501 no 788 0.30113917038081 2372 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS|SITE OF POLARIZED GROWTH|CHROMOSOME|CYTOSKELETON Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress regulation of exit from mitosis|protein phosphorylation kinase regulator activity spindle pole body|cellular bud neck YLR397C YLR397C AFG2 het Verified -0.552157957636812 0.290420068056564 no 789 0.269325448314934 2472 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs ribosomal large subunit biogenesis|response to drug ATPase activity intracellular|preribosome, large subunit precursor YDR201W YDR201W SPC19 het Verified -0.558076707278822 0.28839600268721 no 790 0.248512887843326 2505 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body microtubule nucleation|mitotic spindle organization in nucleus|regulation of microtubule polymerization or depolymerization microtubule binding|structural constituent of cytoskeleton spindle microtubule|condensed nuclear chromosome kinetochore|DASH complex|spindle pole body|spindle YGR029W YGR029W ERV1 het Verified -0.559604949734788 0.287874463978929 no 791 0.294128700173293 2384 NA FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS|RESPONSE TO OXIDATIVE STRESS MITOCHONDRION Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) cellular response to oxidative stress|cellular iron ion homeostasis|protein import into mitochondrial intermembrane space thiol oxidase activity|flavin-linked sulfhydryl oxidase activity mitochondrion|mitochondrial intermembrane space YKL059C YKL059C MPE1 het Verified -0.562334651060681 0.286944016925455 no 792 0.269359170913415 2488 NA FT RNA PROCESSING NUCLEUS Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif mRNA cleavage|mRNA polyadenylation RNA binding mRNA cleavage and polyadenylation specificity factor complex YBL077W_d YBL077W YBL077W het Dubious -0.563679243212508 0.28648622305546 no 793 0.270607579108924 2480 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Unknown Unknown Unknown YOL102C YOL102C TPT1 het Verified -0.566678075970264 0.28546645940485 no 794 0.263832367160887 2458 NA FT RNA PROCESSING NUCLEUS tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide tRNA splicing, via endonucleolytic cleavage and ligation tRNA 2'-phosphotransferase activity nucleus|cytoplasm YDL220C YDL220C CDC13 het Verified -0.56675955080179 0.285438777733313 no 795 0.277219060915475 2424 NA FT CELL CYCLE|CELL DIVISION NUCLEUS|CHROMOSOME Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation telomere capping|telomere maintenance via telomerase|negative regulation of telomerase activity|telomere maintenance|regulation of telomere maintenance via telomerase single-stranded telomeric DNA binding|telomerase inhibitor activity nuclear telomere cap complex YNR046W YNR046W TRM112 het Verified -0.573761385028496 0.283064641370048 no 796 0.247774265641131 2532 NA FT RNA PROCESSING NUCLEUS Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress rRNA (guanine-N7)-methylation|peptidyl-glutamine methylation|tRNA methylation protein methyltransferase activity|zinc ion binding|tRNA (guanine-N2-)-methyltransferase activity|rRNA (guanine) methyltransferase activity eRF1 methyltransferase complex|nucleolus|nucleus|tRNA (m2G10) methyltransferase complex|cytoplasm YDR510W YDR510W SMT3 het Verified -0.578597060637631 0.281430544269588 no 797 0.260654588193284 2477 NA FT NUCLEUS|CHROMOSOME|CYTOSKELETON Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 protein sumoylation protein tag condensed nuclear chromosome|nucleus|septin ring YIL003W YIL003W CFD1 het Verified -0.578623652626217 0.281421570763357 no 798 0.321493405427883 2292 NA FT RNA PROCESSING|COFACTOR METABOLISM Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol iron-sulfur cluster assembly|tRNA wobble uridine modification 4 iron, 4 sulfur cluster binding|ATPase activity cytoplasm YNL131W YNL131W TOM22 het Verified -0.58136394876789 0.280497594733877 no 799 0.328203944984669 2258 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes protein import into mitochondrial matrix|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|integral to mitochondrial outer membrane|mitochondrial outer membrane translocase complex YDR044W YDR044W HEM13 het Verified -0.585527793667903 0.279096441900462 no 800 0.255401369352858 2495 NA FT OXIDATION-REDUCTION PROCESS|COFACTOR METABOLISM Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) heme biosynthetic process coproporphyrinogen oxidase activity cytosol YMR185W YMR185W RTP1 het Verified -0.589015633364469 0.277925393228231 no 801 0.26680667325935 2500 NA Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene biological_process molecular_function cellular_component YML126C YML126C ERG13 het Verified -0.589576214012359 0.277737401167235 no 802 0.271532772997875 2453 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis ergosterol biosynthetic process hydroxymethylglutaryl-CoA synthase activity endoplasmic reticulum YEL035C_p YEL035C UTR5 het Uncharacterized -0.590058901886116 0.277575580406856 no 803 0.299469787379816 2367 NA Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions biological_process molecular_function cellular_component YLR222C YLR222C UTP13 het Verified -0.590410540617872 0.277457722788869 no 804 0.303938282305397 2331 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YCR093W YCR093W CDC39 het Verified -0.590561518733327 0.277407127448419 no 805 0.303607402437036 2338 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING NUCLEUS Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|regulation of transcription from RNA polymerase II promoter|positive regulation of transcription elongation from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|pseudohyphal growth|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter molecular_function CCR4-NOT core complex|nucleus|cytoplasm YDR460W YDR460W TFB3 het Verified -0.590645281484853 0.277379059067891 no 806 0.260914622487701 2478 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair|transcription from RNA polymerase II promoter core RNA polymerase binding transcription factor activity holo TFIIH complex|TFIIK complex YOR168W YOR168W GLN4 het Verified -0.593258524356141 0.276504075625873 no 807 0.2340723912733 2583 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions glutaminyl-tRNA aminoacylation glutamine-tRNA ligase activity cytosol|mitochondrion YHR085W YHR085W IPI1 het Verified -0.594241204683485 0.276175398510666 no 808 0.272417960725743 2439 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene ribosomal large subunit assembly|rRNA processing molecular_function nucleoplasm|nucleus YLR127C YLR127C APC2 het Verified -0.595257272954243 0.275835755895824 no 809 0.244929678594239 2552 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p mitotic metaphase/anaphase transition|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|exit from mitosis|cyclin catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YIL129C YIL129C TAO3 het Verified -0.59554060862924 0.275741081476466 no 810 0.279379082272147 2412 NA FT CELL DIVISION MITOCHONDRION|SITE OF POLARIZED GROWTH Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p cell morphogenesis|cell budding|budding cell apical bud growth molecular_function incipient cellular bud site|mitochondrion|cellular bud|mating projection tip YBR102C YBR102C EXO84 het Verified -0.597123951389679 0.275212313954808 no 811 0.254783818949295 2531 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING SITE OF POLARIZED GROWTH Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate spliceosomal complex assembly|Golgi to plasma membrane transport|exocyst localization|exocytosis|exocyst assembly molecular_function incipient cellular bud site|mating projection tip|cellular bud neck|cellular bud tip|exocyst YHR088W YHR088W RPF1 het Verified -0.59986969958289 0.274296538752009 no 812 0.287403044679979 2402 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|ribosomal large subunit assembly|ribosomal large subunit export from nucleus|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA primary transcript binding nucleolus|preribosome, large subunit precursor YGR179C YGR179C OKP1 het Verified -0.599934910585075 0.27427480756803 no 813 0.263502950608714 2474 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 attachment of spindle microtubules to kinetochore|chromosome segregation molecular_function COMA complex|condensed nuclear chromosome kinetochore YDR489W YDR489W SLD5 het Verified -0.60182211855369 0.273646275136687 no 814 0.27288484232083 2432 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YPR180W YPR180W AOS1 het Verified -0.604241725508231 0.27284147179418 no 815 0.270669548638351 2458 NA FT RESPONSE TO DNA DAMAGE NUCLEUS Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability protein sumoylation SUMO activating enzyme activity nucleus|SUMO activating enzyme complex YGL169W YGL169W SUA5 het Verified -0.609247190557852 0.271180302557369 no 816 0.278336165258459 2410 NA FT RNA PROCESSING|TRANSLATION NUCLEUS|CHROMOSOME Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family threonylcarbamoyladenosine metabolic process|telomere maintenance|regulation of translational fidelity single-stranded telomeric DNA binding cytoplasm YER018C YER018C SPC25 het Verified -0.61016523757198 0.270876177431811 no 817 0.269912165200666 2485 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering microtubule nucleation|chromosome segregation structural constituent of cytoskeleton Ndc80 complex|condensed nuclear chromosome kinetochore|condensed nuclear chromosome, centromeric region YOL022C YOL022C TSR4 het Verified -0.616885744888925 0.268655044990855 no 818 0.242077418045224 2548 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 maturation of SSU-rRNA molecular_function cytoplasm YML091C YML091C RPM2 het Verified -0.619948564043299 0.267645825630913 no 819 0.277577166521057 2412 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING|TRANSLATION|MITOCHONDRION ORGANIZATION NUCLEUS|MITOCHONDRION Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus cytoplasmic translation|tRNA 5'-leader removal|mRNA processing|mitochondrion organization|RNA processing|positive regulation of transcription from RNA polymerase II promoter ribonuclease P activity mitochondrion|nucleus|mitochondrial matrix|cytoplasmic mRNA processing body|mitochondrial ribonuclease P complex YIL109C YIL109C SEC24 het Verified -0.627625375339558 0.265124690774283 no 820 0.246659800113329 2550 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p cargo loading into COPII-coated vesicle signal sequence binding COPII vesicle coat YGL074C_d YGL074C YGL074C het Dubious -0.629862958622442 0.264392124804664 no 821 0.263524907441513 2472 NA Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Unknown Unknown Unknown YDR047W YDR047W HEM12 het Verified -0.63293447887939 0.263388215487012 no 822 0.275175268702104 2448 NA FF|FT COFACTOR METABOLISM NUCLEUS Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda heme biosynthetic process uroporphyrinogen decarboxylase activity nucleus|cytoplasm YLR355C YLR355C ILV5 het Verified -0.634730078892352 0.262802236329433 no 823 0.29659369183851 2380 NA FF|FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION MITOCHONDRION Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids mitochondrial genome maintenance|branched-chain amino acid biosynthetic process double-stranded DNA binding|ketol-acid reductoisomerase activity mitochondrial nucleoid|mitochondrion YNL272C YNL272C SEC2 het Verified -0.63621401668046 0.262318469249332 no 824 0.25033127830144 2551 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT SITE OF POLARIZED GROWTH Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles autophagy|exocytosis guanyl-nucleotide exchange factor activity|phosphatidylinositol-4-phosphate binding cytosol|transport vesicle|mating projection tip|cellular bud neck|cellular bud tip YGL040C YGL040C HEM2 het Verified -0.640355445365844 0.260970770960942 no 825 0.258653929899131 2529 NA FT COFACTOR METABOLISM NUCLEUS Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus heme biosynthetic process porphobilinogen synthase activity nucleus|cytoplasm YGR060W YGR060W ERG25 het Verified -0.640492843934837 0.260926119997326 no 826 0.285361039617261 2420 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|PLASMA MEMBRANE C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol ergosterol biosynthetic process C-4 methylsterol oxidase activity plasma membrane|endoplasmic reticulum membrane YNR026C YNR026C SEC12 het Verified -0.640919477568976 0.260787500199865 no 827 0.247434303429006 2575 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|NUCLEOTIDE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER regulation of COPII vesicle coating Sar guanyl-nucleotide exchange factor activity Golgi apparatus|endoplasmic reticulum YHR188C YHR188C GPI16 het Verified -0.642133464963619 0.260393264516246 no 828 0.23130868509989 2586 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog attachment of GPI anchor to protein GPI-anchor transamidase activity GPI-anchor transamidase complex YKL112W YKL112W ABF1 het Verified -0.645301945273195 0.259365766172752 no 829 0.281201841128633 2401 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair negative regulation of transcription from RNA polymerase II promoter|global genome nucleotide-excision repair|chromatin remodeling|DNA-dependent DNA replication|positive regulation of transcription from RNA polymerase II promoter|chromatin silencing at silent mating-type cassette DNA replication origin binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific DNA binding, bending nucleotide-excision repair factor 4 complex|nucleus YKR025W YKR025W RPC37 het Verified -0.651207357617333 0.257456320732161 no 830 0.249354847899494 2501 NA FT NUCLEUS RNA polymerase III subunit C37 tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YKL141W YKL141W SDH3 het Verified -0.651579038237314 0.257336386940855 no 831 0.251880869509674 2516 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|OXIDATION-REDUCTION PROCESS|MITOCHONDRION ORGANIZATION|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex mitochondrial electron transport, succinate to ubiquinone|cellular respiration|tricarboxylic acid cycle|protein import into mitochondrial inner membrane succinate dehydrogenase (ubiquinone) activity mitochondrial respiratory chain complex II|mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YGR083C YGR083C GCD2 het Verified -0.652457611846981 0.257053004548731 no 832 0.292004356958443 2383 NA FT TRANSLATION Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|guanyl-nucleotide exchange factor activity|translation initiation factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YJR042W YJR042W NUP85 het Verified -0.653139444706721 0.256833192457516 no 833 0.289016090113798 2388 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 positive regulation of transcription, DNA-dependent|mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|nuclear pore distribution structural constituent of nuclear pore integral to membrane|nuclear pore|nuclear pore outer ring YKL083W_d YKL083W YKL083W het Dubious -0.653216951643301 0.256808211645171 no 834 0.265002595788084 2459 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 Unknown Unknown Unknown YDR087C YDR087C RRP1 het Verified -0.653621921361908 0.256677708767412 no 835 0.256295905887213 2513 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles rRNA processing molecular_function nucleolus|nucleus|preribosome, large subunit precursor YMR203W YMR203W TOM40 het Verified -0.654589357437405 0.256366089015202 no 836 0.330130525896811 2282 NA FF|FT PROTEIN LOCALIZATION|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS MITOCHONDRION Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore protein import into mitochondrial matrix protein channel activity mitochondrial outer membrane|mitochondrion|integral to membrane|mitochondrial outer membrane translocase complex YOR287C YOR287C RRP36 het Verified -0.657051482245929 0.255573907056928 no 837 0.227464787138325 2628 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cleavage involved in rRNA processing molecular_function nucleolus|90S preribosome YBR011C YBR011C IPP1 het Verified -0.662823177636601 0.253721903772896 no 838 0.281638028499343 2423 NA Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase phosphate-containing compound metabolic process inorganic diphosphatase activity|protein homodimerization activity cytosol YKL036C_d YKL036C YKL036C het Dubious -0.664204138033193 0.253279832680836 no 839 0.250592082826985 2584 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Unknown Unknown Unknown YNL007C YNL007C SIS1 het Verified -0.669888336100745 0.251464487858372 no 840 0.238894685593144 2553 NA FT CELL CYCLE|TRANSLATION NUCLEUS RIBOSOME Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress protein folding|translational initiation unfolded protein binding cytosolic small ribosomal subunit YHR101C YHR101C BIG1 het Verified -0.670074695716977 0.251405087391993 no 841 0.228668281037093 2586 NA FT CELL WALL ORG/BIOGENESIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan fungal-type cell wall biogenesis molecular_function integral to endoplasmic reticulum membrane YDL141W YDL141W BPL1 het Verified -0.671499976244487 0.250951037298557 no 842 0.222723864593149 2593 NA FT NUCLEUS Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation protein biotinylation biotin-[pyruvate-carboxylase] ligase activity|biotin-[acetyl-CoA-carboxylase] ligase activity nucleus|cytoplasm YOL034W YOL034W SMC5 het Verified -0.67174842317854 0.250871934266163 no 843 0.218406615984779 2610 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair DNA repair|resolution of recombination intermediates|recombinational repair|rDNA separation damaged DNA binding|single-stranded DNA binding|ATPase activity Smc5-Smc6 complex|nucleus YGL018C YGL018C JAC1 het Verified -0.672956613795897 0.250487446626934 no 844 0.236465584865413 2571 NA FT OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix iron-sulfur cluster assembly|aerobic respiration chaperone binding mitochondrion YOL139C YOL139C CDC33 het Verified -0.674720460328433 0.249926691429489 no 845 0.234274175330538 2564 NA FT CELL CYCLE|TRANSLATION NUCLEUS RIBOSOME mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth regulation of cell cycle|translational initiation|nuclear-transcribed mRNA catabolic process, nonsense-mediated decay phosphatidylinositol-3-phosphate binding|translation initiation factor activity ribosome|nucleus|eukaryotic translation initiation factor 4F complex|cytoplasmic stress granule|cytoplasm YPR143W YPR143W RRP15 het Verified -0.67679231257699 0.249268868155345 no 846 0.248615806432348 2540 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|preribosome, large subunit precursor YBL073W_d YBL073W YBL073W het Dubious -0.681747477405271 0.24769932035451 no 847 0.231459146325148 2615 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Unknown Unknown Unknown YCR072C YCR072C RSA4 het Verified -0.682387043842858 0.24749712302705 no 848 0.203983096350104 2698 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus ribosomal large subunit assembly molecular_function ribosome|nucleolus YDR367W YDR367W KEI1 het Verified -0.684384586347945 0.246866173261445 no 849 0.277918767859054 2461 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene inositolphosphoceramide metabolic process inositol phosphoceramide synthase regulator activity integral to membrane|Golgi apparatus|integral to Golgi membrane|inositol phosphoceramide synthase complex|cytoplasm YNL062C YNL062C GCD10 het Verified -0.686507056659191 0.246196707977136 no 850 0.27715668294763 2440 NA FT RNA PROCESSING|TRANSLATION NUCLEUS Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression tRNA methylation tRNA (adenine-N1-)-methyltransferase activity tRNA (m1A) methyltransferase complex|nucleus YDL098C YDL098C SNU23 het Verified -0.687235870682882 0.245967051678104 no 851 0.23248107987733 2587 NA FT RNA PROCESSING NUCLEUS Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YDR301W YDR301W CFT1 het Verified -0.687249991138127 0.24596260332243 no 852 0.250607607247425 2538 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS|MITOCHONDRION RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF mRNA cleavage|termination of RNA polymerase II transcription|mRNA polyadenylation RNA binding mRNA cleavage factor complex|mitochondrion|mRNA cleavage and polyadenylation specificity factor complex YDR238C YDR238C SEC26 het Verified -0.690000648286571 0.245096889832534 no 853 0.282588878265729 2422 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) ER to Golgi vesicle-mediated transport molecular_function coated vesicle|COPI vesicle coat YGL113W YGL113W SLD3 het Verified -0.690626141208067 0.244900257592926 no 854 0.275165523557277 2437 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress DNA unwinding involved in replication|double-strand break repair via break-induced replication|DNA replication initiation chromatin binding nuclear chromatin|DNA replication preinitiation complex YKR081C YKR081C RPF2 het Verified -0.696114145625928 0.243178674220504 no 855 0.263927690348076 2480 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles ribosomal large subunit assembly|maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) 7S RNA binding|5S rRNA binding|rRNA binding nucleolus|preribosome, large subunit precursor YGR216C YGR216C GPI1 het Verified -0.699052739554829 0.242259536065588 no 856 0.205230830744029 2642 NA FT LIPID METABOLISM ENDOPLASMIC RETICULUM Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs GPI anchor biosynthetic process phosphatidylinositol N-acetylglucosaminyltransferase activity glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex|integral to membrane YNL182C YNL182C IPI3 het Verified -0.699577429838074 0.242095620931482 no 857 0.224811507821095 2619 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene ribosomal large subunit assembly|rRNA processing molecular_function nucleoplasm|nucleus YKL012W YKL012W PRP40 het Verified -0.700782597888557 0.241719349901845 no 858 0.216502640943282 2682 NA FT RNA PROCESSING NUCLEUS U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex mRNA splicing, via spliceosome RNA binding U1 snRNP|nucleus|U2-type prespliceosome YNL026W YNL026W SAM50 het Verified -0.703248606840297 0.240950416240936 no 859 0.224039596002002 2622 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 protein complex assembly|protein import into mitochondrial outer membrane protein channel activity mitochondrial outer membrane|mitochondrial sorting and assembly machinery complex|mitochondrion|integral to mitochondrial outer membrane YGL116W YGL116W CDC20 het Verified -0.705175777899956 0.240350426583624 no 860 0.271990114340902 2446 NA FF|FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress spindle checkpoint|activation of mitotic anaphase-promoting complex activity|meiotic anaphase I|positive regulation of protein catabolic process|activation of anaphase-promoting complex activity involved in meiotic cell cycle|positive regulation of mitotic metaphase/anaphase transition|cyclin catabolic process anaphase-promoting complex binding|ubiquitin-protein ligase activator activity mitotic checkpoint complex|anaphase-promoting complex YML092C YML092C PRE8 het Verified -0.71051289027373 0.238693070144072 no 861 0.248301726338931 2522 NA FF|FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Alpha 2 subunit of the 20S proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function mitochondrion|proteasome core complex, alpha-subunit complex|proteasome storage granule|nucleus|nuclear outer membrane-endoplasmic reticulum membrane network YGL112C YGL112C TAF6 het Verified -0.719384492973492 0.235952024373904 no 862 0.259914433948724 2513 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|RNA polymerase II activating transcription factor binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YDR373W YDR373W FRQ1 het Verified -0.719802995948973 0.235823150025729 no 863 0.225609310340645 2600 NA FT SIGNALING ENDOMEMBRANE SYSTEM|GOLGI APPARATUS|PLASMA MEMBRANE N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily regulation of signal transduction enzyme activator activity|calcium ion binding Golgi membrane YDL092W YDL092W SRP14 het Verified -0.72009154701817 0.235734315843155 no 864 0.258081100141045 2498 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION NUCLEUS Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding signal recognition particle, endoplasmic reticulum targeting YNL181W_p YNL181W YNL181W het Uncharacterized -0.723026762287641 0.234831721190401 no 865 0.188659265003993 2754 NA FT OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Putative oxidoreductase; required for cell viability biological_process oxidoreductase activity|oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor nuclear envelope|endoplasmic reticulum YJL008C YJL008C CCT8 het Verified -0.724010855955005 0.234529535167709 no 866 0.219974566136461 2656 NA Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex|cytoplasm YJR065C YJR065C ARP3 het Verified -0.724629649599634 0.234339632171962 no 867 0.223829415491626 2671 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION CYTOSKELETON Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity mitochondrion inheritance|Arp2/3 complex-mediated actin nucleation|actin filament organization ATP binding Arp2/3 protein complex|plasma membrane YHR019C YHR019C DED81 het Verified -0.73398069192318 0.231480252907858 no 868 0.272210332502377 2453 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA asparaginyl-tRNA aminoacylation asparagine-tRNA ligase activity cytosol YNL240C YNL240C NAR1 het Verified -0.734335998040709 0.231371991694684 no 869 0.229999371878309 2626 NA FT COFACTOR METABOLISM NUCLEUS Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf iron-sulfur cluster assembly iron-sulfur cluster binding|ferredoxin hydrogenase activity cytosol|membrane YPL117C YPL117C IDI1 het Verified -0.73516159462958 0.23112054278666 no 870 0.219309566443064 2690 NA FT LIPID METABOLISM NUCLEUS Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability farnesyl diphosphate biosynthetic process isopentenyl-diphosphate delta-isomerase activity nucleus|cytoplasm YDL196W_d YDL196W YDL196W het Dubious -0.741226903257631 0.229277936881472 no 871 0.302277404041391 2354 NA FF Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Unknown Unknown Unknown YDR052C YDR052C DBF4 het Verified -0.742320077816328 0.228946714939501 no 872 0.248160170688955 2512 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION|CHROMOSOME SEGREGATION Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress negative regulation of exit from mitosis|DNA replication initiation|positive regulation of protein kinase activity DNA replication origin binding|protein serine/threonine kinase activator activity Dbf4-dependent protein kinase complex YLR002C YLR002C NOC3 het Verified -0.746122896743695 0.227796589514533 no 873 0.207021335356215 2675 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION NUCLEUS Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing rRNA processing|DNA replication initiation chromatin binding nucleoplasm|nucleolus|Noc2p-Noc3p complex|nucleus YML064C YML064C TEM1 het Verified -0.747183866220452 0.227476290756265 no 874 0.221564532455008 2618 NA FT CELL CYCLE|SIGNALING|CELL DIVISION CYTOSKELETON GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis regulation of exit from mitosis protein binding|GTPase activity spindle pole body YDL102W YDL102W POL3 het Verified -0.749688473653444 0.226721175579624 no 875 0.226388767467082 2603 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) lagging strand elongation|RNA-dependent DNA replication|base-excision repair|postreplication repair|DNA replication, removal of RNA primer|nucleotide-excision repair|mismatch repair|maintenance of fidelity involved in DNA-dependent DNA replication|DNA replication|leading strand elongation single-stranded DNA specific 3'-5' exodeoxyribonuclease activity|DNA-directed DNA polymerase activity delta DNA polymerase complex|replication fork YCL041C_d YCL041C YCL041C het Dubious -0.750197079136571 0.226568008858006 no 876 0.222297518073831 2641 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Unknown Unknown Unknown YLR167W YLR167W RPS31 het Verified -0.751953559287235 0.226039493685068 no 877 0.235409731335601 2576 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|TRANSLATION NUCLEUS RIBOSOME Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog cytoplasmic translation|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S)|ribosomal small subunit assembly|ribosome biogenesis structural constituent of ribosome|protein tag cytosolic small ribosomal subunit|cytoplasm YGR024C YGR024C THG1 het Verified -0.75212224220164 0.225988774630454 no 878 0.217955278500847 2673 NA FT RNA PROCESSING NUCLEUS tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes tRNA modification tRNA guanylyltransferase activity nucleus|cytoplasm YER043C YER043C SAH1 het Verified -0.760475352788303 0.223485248271076 no 879 0.20809917551423 2677 NA FT KETONE METABOLISM|AMINO ACID METABOLISM S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor methionine metabolic process|selenocysteine metabolic process adenosylhomocysteinase activity cytoplasm YGR074W YGR074W SMD1 het Verified -0.764104749461996 0.222402411102288 no 880 0.212302534605715 2673 NA FT RNA PROCESSING NUCLEUS Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U4/U6 x U5 tri-snRNP complex|U2-type prespliceosome|U5 snRNP YPR033C YPR033C HTS1 het Verified -0.765267787470907 0.222056050743463 no 881 0.217383475867179 2676 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome histidyl-tRNA aminoacylation|mitochondrial translation aminoacyl-tRNA ligase activity mitochondrion|cytoplasm YPL153C YPL153C RAD53 het Verified -0.767336773756107 0.221440654813299 no 882 0.229794099129602 2593 NA FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|NUCLEOTIDE METABOLISM|PROTEIN PHOSPHORYLATION NUCLEUS Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication DNA repair|protein localization|protein phosphorylation|nucleobase-containing compound metabolic process|DNA damage checkpoint|DNA replication initiation|deoxyribonucleoside triphosphate biosynthetic process protein serine/threonine/tyrosine kinase activity|DNA replication origin binding|protein kinase activity nucleus YJR068W YJR068W RFC2 het Verified -0.774302236269104 0.219376040776032 no 883 0.193058947695932 2720 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon mismatch repair|sister chromatid cohesion|cell cycle checkpoint|leading strand elongation DNA clamp loader activity|purine nucleotide binding Elg1 RFC-like complex|DNA replication factor C complex|Rad17 RFC-like complex|Ctf18 RFC-like complex YML098W YML098W TAF13 het Verified -0.779408876350269 0.217869448351993 no 884 0.188922785369996 2711 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YPL251W_d YPL251W YPL251W het Dubious -0.784443290199016 0.216390022668725 no 885 0.204317355127425 2701 NA Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Unknown Unknown Unknown YKR063C YKR063C LAS1 het Verified -0.78783759921819 0.21539585251349 no 886 0.200963286764698 2708 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability maturation of LSU-rRNA|maturation of 5.8S rRNA molecular_function mitochondrion|nucleus|preribosome|cytoplasm YMR220W YMR220W ERG8 het Verified -0.793445619796107 0.213759120273816 no 887 0.214968080084027 2660 NA FT LIPID METABOLISM|COFACTOR METABOLISM NUCLEUS Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate organic acid phosphorylation|ergosterol biosynthetic process|isopentenyl diphosphate biosynthetic process, mevalonate pathway|farnesyl diphosphate biosynthetic process, mevalonate pathway phosphomevalonate kinase activity nucleus|cytoplasm YLR105C YLR105C SEN2 het Verified -0.799234362481648 0.212077265224101 no 888 0.190438233271031 2718 NA FT RNA PROCESSING ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity mitochondrial outer membrane|mitochondrion|tRNA-intron endonuclease complex YOR160W YOR160W MTR10 het Verified -0.802377543981044 0.2111672994262 no 889 0.206091958251627 2713 NA FT PROTEIN LOCALIZATION|RNA LOCALIZATION NUCLEUS Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress RNA localization|tRNA transport|RNA import into nucleus|protein import into nucleus|mRNA-binding (hnRNP) protein import into nucleus nuclear localization sequence binding integral to membrane|nucleus|cytoplasm YER023W YER023W PRO3 het Verified -0.803792484949112 0.210758415319791 no 890 0.179282943992274 2770 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|OXIDATION-REDUCTION PROCESS Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis proline biosynthetic process pyrroline-5-carboxylate reductase activity cytoplasm YOR119C YOR119C RIO1 het Verified -0.804113386170473 0.210665747185911 no 891 0.209644058258198 2671 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA regulation of exit from mitosis|S phase of mitotic cell cycle|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) protein kinase activity nucleus|cytoplasm YDR394W YDR394W RPT3 het Verified -0.805077236128922 0.210387555530665 no 892 0.257122320081084 2482 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|proteasome regulatory particle assembly|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex YML023C YML023C NSE5 het Verified -0.806262506536125 0.210045752138605 no 893 0.176563852358475 2738 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair molecular_function Smc5-Smc6 complex|nucleus YMR049C YMR049C ERB1 het Verified -0.811892529254916 0.208426650788617 no 894 0.212006864868072 2656 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 ribosomal large subunit biogenesis|rRNA processing molecular_function PeBoW complex|nucleolus|nucleus|preribosome, large subunit precursor YGL102C_d YGL102C YGL102C het Dubious -0.822266198231068 0.205462705052722 no 895 0.209755653973229 2679 NA Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Unknown Unknown Unknown YGL068W YGL068W MNP1 het Verified -0.823734915098684 0.205045098010879 no 896 0.196250287348159 2733 NA FT TRANSLATION|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth aerobic respiration structural constituent of ribosome mitochondrial nucleoid|mitochondrion YNL163C YNL163C RIA1 het Verified -0.829630657526528 0.203373818910399 no 897 0.187149926484779 2721 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes mature ribosome assembly GTPase activity cytoplasm YNR017W YNR017W TIM23 het Verified -0.837444862145037 0.20117127883484 no 898 0.202657203654923 2718 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel protein import into mitochondrial matrix mitochondrion targeting sequence binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane presequence translocase complex YNL061W YNL061W NOP2 het Verified -0.85116584742168 0.197338615761037 no 899 0.211679110042963 2650 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles rRNA processing S-adenosylmethionine-dependent methyltransferase activity|RNA methyltransferase activity nucleolus|preribosome, large subunit precursor YER112W YER112W LSM4 het Verified -0.852403608217144 0.196995059098653 no 900 0.223050855248754 2634 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress nuclear-transcribed mRNA catabolic process|mRNA splicing, via spliceosome|cytoplasmic mRNA processing body assembly U6 snRNA binding U6 snRNP|nucleolus|small nucleolar ribonucleoprotein complex|cytoplasmic mRNA processing body|U4/U6 x U5 tri-snRNP complex YMR290W-A_d YMR290W-A YMR290W-A het Dubious -0.85673596089096 0.195795412233231 no 901 0.257398565781591 2553 NA FF Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Unknown Unknown Unknown YGL172W YGL172W NUP49 het Verified -0.858423314527654 0.195329379113923 no 902 0.227034549580199 2641 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) poly(A)+ mRNA export from nucleus|ribosomal large subunit export from nucleus|protein import into nucleus|tRNA export from nucleus nucleocytoplasmic transporter activity|structural constituent of nuclear pore nuclear pore central transport channel|nuclear envelope|integral to membrane|nuclear pore YDR113C YDR113C PDS1 het Verified -0.860572181110641 0.194736856500325 no 903 0.201594170318751 2696 NA FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CYTOSKELETON Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation protein localization|recombinational repair|meiosis I|mitotic sister chromatid segregation enzyme binding nucleus|cytoplasm|spindle YKR002W YKR002W PAP1 het Verified -0.8695063977173 0.192285105136933 no 904 0.195760252946485 2718 NA FT RNA PROCESSING NUCLEUS Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping mRNA polyadenylation|snoRNA polyadenylation polynucleotide adenylyltransferase activity nucleoplasm|mRNA cleavage and polyadenylation specificity factor complex YNL188W YNL188W KAR1 het Verified -0.872688441968123 0.191416459618565 no 905 0.193749187258275 2704 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p karyogamy involved in conjugation with cellular fusion|spindle pole body duplication associated with nuclear envelope protein binding half bridge of spindle pole body YMR028W YMR028W TAP42 het Verified -0.874109228879246 0.1910293860232 no 906 0.188872778173876 2734 NA FT SIGNALING Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits TOR signaling cascade|positive regulation of transcription from RNA polymerase I promoter molecular_function cytosol|extrinsic to membrane YJL041W YJL041W NSP1 het Verified -0.874811087287063 0.190838351747983 no 907 0.188982529410366 2751 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RIBOSOME BIOGENESIS|RNA LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS NUCLEAR PORE FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress poly(A)+ mRNA export from nucleus|NLS-bearing substrate import into nucleus|ncRNA export from nucleus|ribosomal large subunit export from nucleus|tRNA export from nucleus|protein import into nucleus|ribosomal small subunit export from nucleus nucleocytoplasmic transporter activity|phospholipid binding|structural constituent of nuclear pore nuclear pore central transport channel|integral to membrane|nuclear pore nuclear basket|nuclear pore YDR416W YDR416W SYF1 het Verified -0.880525359280479 0.189287386770859 no 908 0.150237285913998 2849 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING NUCLEUS Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans generation of catalytic spliceosome for first transesterification step first spliceosomal transesterification activity|second spliceosomal transesterification activity U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 1 spliceosome|U2-type catalytic step 2 spliceosome YJR012C_p YJR012C YJR012C het Uncharacterized -0.910342465863713 0.181320964810378 no 909 0.148419341201624 2880 NA Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W biological_process molecular_function cellular_component YFL034C-B YFL034C-B MOB2 het Verified -0.915342942224982 0.180005811069405 no 910 0.134646102395574 2900 NA FT CELL CYCLE|CELL DIVISION|PROTEIN PHOSPHORYLATION NUCLEUS|SITE OF POLARIZED GROWTH Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p positive regulation of protein phosphorylation|establishment or maintenance of cell polarity|Unknown|budding cell apical bud growth protein kinase activator activity nucleus|cellular bud|cellular bud neck|mating projection tip|cellular bud tip YCR013C_d YCR013C YCR013C het Dubious -0.920362891202771 0.178691576942611 no 911 0.145668192786855 2851 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Unknown Unknown Unknown YOL078W YOL078W AVO1 het Verified -0.927980205270823 0.176708918857144 no 912 0.141532755646264 2891 NA FT SIGNALING|CELL WALL ORG/BIOGENESIS|CYTOSKELETON ORGANIZATION PLASMA MEMBRANE Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth TOR signaling cascade|fungal-type cell wall organization|regulation of cell growth|negative regulation of Ras protein signal transduction|establishment or maintenance of actin cytoskeleton polarity phosphatidylinositol-4,5-bisphosphate binding|Ras GTPase binding TORC2 complex|plasma membrane|cytoplasm YDL140C YDL140C RPO21 het Verified -0.929915625519147 0.176207386013975 no 913 0.148723003191061 2890 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS|MITOCHONDRION RNA POL II, HOLOENZYME RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity mitochondrion|DNA-directed RNA polymerase II, core complex YDL029W YDL029W ARP2 het Verified -0.933610859905577 0.175252330674078 no 914 0.18905533367221 2744 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|CELL WALL ORG/BIOGENESIS|NUCLEOTIDE METABOLISM|MITOCHONDRION ORGANIZATION|CYTOSKELETON ORGANIZATION|VACUOLAR TRANSPORT MITOCHONDRION|CYTOSKELETON Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity mitochondrion inheritance|Arp2/3 complex-mediated actin nucleation|ascospore wall assembly|actin filament organization|establishment of mitochondrion localization|ATP catabolic process|CVT pathway ATPase activity|ATP binding Arp2/3 protein complex|mitochondrion YLR140W_d YLR140W YLR140W het Dubious -0.9449033843246 0.172354101789052 no 915 0.177784884345329 2771 NA Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Unknown Unknown Unknown YOR207C YOR207C RET1 het Verified -0.950541603757794 0.170918562368502 no 916 0.20417305949246 2702 NA FF|FT NUCLEUS Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs tRNA transcription from RNA polymerase III promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase III complex YLR291C YLR291C GCD7 het Verified -0.960165413083163 0.168485985505506 no 917 0.169267579290967 2824 NA FT TRANSLATION MITOCHONDRION Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation enzyme regulator activity|translation initiation factor activity|guanyl-nucleotide exchange factor activity mitochondrion|eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YEL058W YEL058W PCM1 het Verified -0.96261578034583 0.167870187680466 no 918 0.105657131500982 2964 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM NUCLEUS Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors fungal-type cell wall chitin biosynthetic process phosphoacetylglucosamine mutase activity nucleus|cytoplasm YLR101C_d YLR101C YLR101C het Dubious -0.962631043942908 0.167866356357109 no 919 0.129035049429146 2911 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W Unknown Unknown Unknown YOR254C YOR254C SEC63 het Verified -0.965579736505735 0.167127259489568 no 920 0.155196319062372 2855 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM|MITOCHONDRION Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER posttranslational protein targeting to membrane, translocation|cytosol to ER transport|SRP-dependent cotranslational protein targeting to membrane|posttranslational protein targeting to membrane protein transporter activity mitochondrion|integral to membrane|Sec62/Sec63 complex|endoplasmic reticulum membrane YLR153C YLR153C ACS2 het Verified -0.970986342598961 0.165777539811652 no 921 0.201385270438554 2700 NA FT CHROMATIN ORGANIZATION|COFACTOR METABOLISM NUCLEUS Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions histone acetylation|acetyl-CoA biosynthetic process|replicative cell aging acetate-CoA ligase activity|acid-ammonia (or amide) ligase activity cytosol|nucleolus|nucleus YHR122W YHR122W CIA2 het Verified -0.971548953902658 0.165637493938891 no 922 0.157830365138286 2825 NA FT CELL CYCLE|CHROMOSOME SEGREGATION NUCLEUS Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B establishment of mitotic sister chromatid cohesion molecular_function nucleus|cytoplasm YML010W YML010W SPT5 het Verified -0.971602889075266 0.165624072351644 no 923 0.150906916793042 2842 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RESPONSE TO DNA DAMAGE|RNA LOCALIZATION NUCLEUS|MITOCHONDRION|CHROMOSOME Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair 7-methylguanosine mRNA capping|intracellular mRNA localization|mRNA splicing, via spliceosome|positive regulation of transcription elongation from RNA polymerase II promoter|Unknown|regulation of transcription-coupled nucleotide-excision repair core RNA polymerase I binding transcription factor activity|core RNA polymerase II binding transcription factor activity|rRNA binding|RNA polymerase II core binding|RNA polymerase I transcription factor binding|RNA polymerase I core binding DSIF complex|mitochondrion|rDNA heterochromatin|nucleus YPL252C YPL252C YAH1 het Verified -0.98205436950414 0.163036531855855 no 924 0.188951864373402 2737 NA FT KETONE METABOLISM|OXIDATION-REDUCTION PROCESS|GEN. OF PRECURSOR METABOLITES AND ENERGY|COFACTOR METABOLISM MITOCHONDRION Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin ubiquinone biosynthetic process|iron-sulfur cluster assembly|heme a biosynthetic process oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor mitochondrial matrix YOL149W YOL149W DCP1 het Verified -0.990803690282544 0.160890723499863 no 925 0.165310881393921 2863 NA FT RNA PROCESSING Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress deadenylation-dependent decapping of nuclear-transcribed mRNA mRNA binding|enzyme activator activity|m7G(5')pppN diphosphatase activity cytoplasmic mRNA processing body YDR498C YDR498C SEC20 het Verified -0.997972442362131 0.159146360891367 no 926 0.154382144607772 2869 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p retrograde vesicle-mediated transport, Golgi to ER|vesicle fusion with endoplasmic reticulum SNAP receptor activity SNARE complex|integral to membrane|endoplasmic reticulum|peroxisome YBR265W YBR265W TSC10 het Verified -1.00744681097063 0.156860052866112 no 927 0.171082759639285 2814 NA FT KETONE METABOLISM|LIPID METABOLISM|OXIDATION-REDUCTION PROCESS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|MITOCHONDRION 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family 3-keto-sphinganine metabolic process|sphingolipid biosynthetic process oxidoreductase activity, acting on NADH or NADPH mitochondrial outer membrane|endoplasmic reticulum|cytoplasm YOL005C YOL005C RPB11 het Verified -1.0108125793616 0.156053070619637 no 928 0.169682180006937 2836 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit termination of RNA polymerase II transcription|transcription from RNA polymerase II promoter RNA-directed RNA polymerase activity|DNA-directed RNA polymerase activity DNA-directed RNA polymerase II, core complex YLR298C YLR298C YHC1 het Verified -1.01674180993613 0.154638135242074 no 929 0.145493940127555 2901 NA FT RNA PROCESSING NUCLEUS Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YDR449C YDR449C UTP6 het Verified -1.01744309822828 0.154471344164232 no 930 0.208552949686081 2663 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) snoRNA binding small-subunit processome|nucleolus|Pwp2p-containing subcomplex of 90S preribosome|90S preribosome YKL049C YKL049C CSE4 het Verified -1.02093249432248 0.153643211332989 no 931 0.11029744806042 2988 NA FT CELL CYCLE|CHROMATIN ORGANIZATION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME KINETOCHORE Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions 2-micrometer plasmid partitioning|mitotic sister chromatid segregation sequence-specific DNA binding|centromeric DNA binding extrachromosomal circular DNA|nuclear nucleosome|condensed nuclear chromosome, centromeric region|centromere-specific nucleosome|kinetochore YBL041W YBL041W PRE7 het Verified -1.02941847962718 0.151641534500981 no 932 0.15595324292462 2836 NA FT PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 6 subunit of the 20S proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasomal ubiquitin-independent protein catabolic process molecular_function nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YGL171W YGL171W ROK1 het Verified -1.03163891526603 0.151120651489347 no 933 0.156914928804897 2857 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS|RNA LOCALIZATION NUCLEUS RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|snoRNA localization RNA-dependent ATPase activity nucleolus|90S preribosome YMR059W YMR059W SEN15 het Verified -1.03713446221265 0.149836598129223 no 934 0.116020274190704 2951 NA FT RNA PROCESSING ENDOMEMBRANE SYSTEM|NUCLEUS|MITOCHONDRION Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p tRNA-type intron splice site recognition and cleavage tRNA-intron endonuclease activity tRNA-intron endonuclease complex YOR260W YOR260W GCD1 het Verified -1.04707910511085 0.147531547708252 no 935 0.172595904702342 2816 NA FT TRANSLATION Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression regulation of translational initiation translation initiation factor activity|guanyl-nucleotide exchange factor activity eukaryotic translation initiation factor 2B complex|guanyl-nucleotide exchange factor complex YLR163C YLR163C MAS1 het Verified -1.04811180931994 0.147293548002672 no 936 0.220289686991451 2630 NA FF|FT PROTEIN LOCALIZATION|PROTEOLYSIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins protein processing involved in protein targeting to mitochondrion metalloendopeptidase activity mitochondrion|mitochondrial processing peptidase complex YIR012W YIR012W SQT1 het Verified -1.04972204743687 0.14692296203028 no 937 0.129524708478386 2935 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS RIBOSOME Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress ribosomal large subunit assembly molecular_function cytosol|cytosolic ribosome|cytoplasm YKL104C YKL104C GFA1 het Verified -1.07062895412475 0.142168149610993 no 938 0.149578593871522 2932 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication fungal-type cell wall chitin biosynthetic process glutamine-fructose-6-phosphate transaminase (isomerizing) activity cellular_component YDR299W YDR299W BFR2 het Verified -1.0792173197668 0.140245430237315 no 939 0.105847860271629 2995 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock rRNA processing molecular_function nucleolus|90S preribosome YLR277C YLR277C YSH1 het Verified -1.07982133452889 0.140110874419049 no 940 0.128649951114919 2955 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication termination of RNA polymerase II transcription|mRNA cleavage|mRNA polyadenylation|RNA splicing|snoRNA splicing|snoRNA 3'-end processing endoribonuclease activity|RNA binding mRNA cleavage factor complex|mRNA cleavage and polyadenylation specificity factor complex YDL205C YDL205C HEM3 het Verified -1.08128174564449 0.139785902450696 no 941 0.142911497696787 2891 NA FT COFACTOR METABOLISM NUCLEUS Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme heme biosynthetic process hydroxymethylbilane synthase activity nucleus|cytoplasm YLR033W YLR033W RSC58 het Verified -1.08353552241967 0.139285396277705 no 942 0.271701804502023 2483 NA FF|FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance nucleosome disassembly|transcription elongation from RNA polymerase II promoter|G1/S transition of mitotic cell cycle|chromatin remodeling molecular_function RSC complex YDR062W YDR062W LCB2 het Verified -1.09527846348007 0.136697329856948 no 943 0.149603958279414 2858 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine sphingolipid biosynthetic process serine C-palmitoyltransferase activity SPOTS complex|serine C-palmitoyltransferase complex YLR314C YLR314C CDC3 het Verified -1.09932811670217 0.135812486277251 no 944 0.129754822755345 2931 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION SITE OF POLARIZED GROWTH|CYTOSKELETON Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells septin ring disassembly|maintenance of cell polarity|cell cycle cytokinesis|septin ring assembly structural molecule activity|GTP binding|1-phosphatidylinositol binding mating projection base|septin filament array|cellular bud neck septin ring|septin complex|prospore membrane|ascospore wall YGL065C YGL065C ALG2 het Verified -1.10391093934034 0.134815886517036 no 945 0.123535666482449 2936 NA FT LIPID METABOLISM|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol oligosaccharide-lipid intermediate biosynthetic process GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity|glycolipid 6-alpha-mannosyltransferase activity integral to membrane|endoplasmic reticulum YCR012W YCR012W PGK1 het Verified -1.11874357119721 0.131624775631727 no 946 0.0861551288576406 3085 NA FT CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY MITOCHONDRION 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis gluconeogenesis|glycolysis phosphoglycerate kinase activity mitochondrion|plasma membrane|cytoplasm YFL045C YFL045C SEC53 het Verified -1.11891299281524 0.131588629732873 no 947 0.117870214302538 2963 NA FT PROTEIN LOCALIZATION|CARBOHYDRATE METABOLISM Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen protein targeting to ER phosphomannomutase activity cytosol YDR002W YDR002W YRB1 het Verified -1.12640393032105 0.129997291970873 no 948 0.146534159819059 2903 NA FT PROTEIN LOCALIZATION|CELL CYCLE|PROTEOLYSIS|RNA LOCALIZATION NUCLEUS Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 RNA export from nucleus|protein import into nucleus|G1/S transition of mitotic cell cycle|ubiquitin-dependent protein catabolic process Ran GTPase binding nucleus|cytoplasm YMR134W YMR134W ERG29 het Verified -1.13422474710484 0.128350142963163 no 949 0.130471768247647 2881 NA FT LIPID METABOLISM|ION HOMEOSTASIS|MITOCHONDRION ORGANIZATION|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes cellular iron ion homeostasis|mitochondrion organization molecular_function nuclear envelope|endoplasmic reticulum|cytoplasm YOR077W YOR077W RTS2 het Verified -1.13939689099292 0.127268826058385 no 950 0.0953426613411332 3032 NA FT NUCLEUS Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication biological_process molecular_function nucleus|cytoplasm YDR086C YDR086C SSS1 het Verified -1.14487616955369 0.126130228419788 no 951 0.135363768405389 2952 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p posttranslational protein targeting to membrane, translocation|SRP-dependent cotranslational protein targeting to membrane, translocation structural molecule activity|P-P-bond-hydrolysis-driven protein transmembrane transporter activity Sec61 translocon complex|Ssh1 translocon complex YDR341C YDR341C YDR341C het Verified -1.15366080009173 0.124319630491228 no 952 0.135516809185149 2955 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies cytoplasmic translation|arginyl-tRNA aminoacylation arginine-tRNA ligase activity mitochondrion|cytoplasm YFL017W-A YFL017W-A SMX2 het Verified -1.16261234737811 0.122493409420722 no 953 0.105381892208348 3004 NA FT RNA PROCESSING NUCLEUS Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G mRNA splicing, via spliceosome molecular_function U1 snRNP|U2-type prespliceosome|U4/U6 x U5 tri-snRNP complex|U5 snRNP YLR117C YLR117C CLF1 het Verified -1.16623602799162 0.121759514873669 no 954 0.0888352180722702 3045 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING NUCLEUS|CHROMOSOME Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins cis assembly of pre-catalytic spliceosome|DNA replication initiation DNA replication origin binding|chromatin binding|first spliceosomal transesterification activity|second spliceosomal transesterification activity chromatin|U2-type post-mRNA release spliceosomal complex|Prp19 complex|U2-type prespliceosome|U2-type catalytic step 2 spliceosome|U2-type catalytic step 1 spliceosome YJL002C YJL002C OST1 het Verified -1.16653824092601 0.121698448348643 no 955 0.104687963236081 2992 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation via asparagine|protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex YHR107C YHR107C CDC12 het Verified -1.17524625582921 0.119948104654861 no 956 0.137985350097807 2960 NA FF|FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION|PROTEIN PHOSPHORYLATION SITE OF POLARIZED GROWTH|CYTOSKELETON Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells maintenance of cell polarity|cell cycle cytokinesis|positive regulation of protein kinase activity|septin ring assembly protein complex scaffold|structural molecule activity|GTP binding|GTPase activity|1-phosphatidylinositol binding septin filament array|cellular bud neck septin ring|septin complex|cytoplasm|mating projection YLR340W YLR340W RPP0 het Verified -1.18875387760285 0.117268266215467 no 957 0.146142501769196 2885 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|TRANSLATION RIBOSOME Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 translational elongation|cytoplasmic translation|ribosomal large subunit assembly structural constituent of ribosome|LSU rRNA binding cytosolic large ribosomal subunit|preribosome, large subunit precursor|90S preribosome|cytoplasm YGL155W YGL155W CDC43 het Verified -1.19518916792562 0.116006564858338 no 958 0.0992321337536722 3025 NA FT SIGNALING|ION HOMEOSTASIS|ION HOMEOSTASIS|PROTEIN PRENYLATION Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis cellular calcium ion homeostasis|protein geranylgeranylation|establishment of cell polarity|small GTPase mediated signal transduction protein geranylgeranyltransferase activity|CAAX-protein geranylgeranyltransferase activity CAAX-protein geranylgeranyltransferase complex|intracellular YPR010C YPR010C RPA135 het Verified -1.20084567576672 0.114905535097487 no 959 0.0976425653932179 3065 NA FT NUCLEUS RNA polymerase I second largest subunit A135 transcription of nuclear large rRNA transcript from RNA polymerase I promoter DNA-directed RNA polymerase activity DNA-directed RNA polymerase I complex YLR215C YLR215C CDC123 het Verified -1.20299753100876 0.114488637995076 no 960 0.0972957650986146 3033 NA FT CELL CYCLE|TRANSLATION|CELL DIVISION Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein regulation of mitotic cell cycle|regulation of translation molecular_function cytoplasm YFR029W YFR029W PTR3 het Verified -1.21076326743142 0.112993073452768 no 961 0.0885725831390201 3082 NA FT PLASMA MEMBRANE Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes response to amino acid stimulus molecular_function extrinsic to plasma membrane YPL190C YPL190C NAB3 het Verified -1.21792798785354 0.111625669700108 no 962 0.100315105636711 3021 NA FT TRANSCRIPTION FROM RNA POL II|RNA PROCESSING NUCLEUS RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing nuclear mRNA surveillance|mRNA 3'-end processing|antisense RNA transcript catabolic process|CUT catabolic process|termination of RNA polymerase II transcription, exosome-dependent|tRNA 3'-end processing|snRNA 3'-end processing|snoRNA 3'-end processing RNA binding|transcription regulatory region RNA binding Nrd1 complex|nucleus YKL042W YKL042W SPC42 het Verified -1.23850677238415 0.107764106248001 no 963 0.142347462322183 2906 NA FT CELL CYCLE|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane microtubule nucleation|spindle pole body duplication associated with nuclear envelope structural constituent of cytoskeleton central plaque of spindle pole body|intermediate layer of spindle pole body YML114C YML114C TAF8 het Verified -1.24510565108718 0.106546458578544 no 964 0.105600827402107 3002 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation RNA polymerase II transcriptional preinitiation complex assembly|transcription from RNA polymerase II promoter RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly nucleus|transcription factor TFIID complex YBR055C YBR055C PRP6 het Verified -1.25373594283664 0.104968999530643 no 965 0.0939590825263434 2988 NA FT RNA PROCESSING NUCLEUS Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YPR034W YPR034W ARP7 het Verified -1.25466414811004 0.104800352910872 no 966 0.199059041131429 2738 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation nucleosome mobilization|ATP-dependent chromatin remodeling|chromatin organization|nucleosome disassembly|transcription elongation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA translocase activity RSC complex|SWI/SNF complex|nucleus YIL091C YIL091C UTP25 het Verified -1.25902696147266 0.104010296817567 no 967 0.0973221589692845 3026 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis ribosomal small subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) U3 snoRNA binding|rRNA binding small-subunit processome|nucleolus|nucleus YER171W YER171W RAD3 het Verified -1.28621894447362 0.0991833306364127 no 968 0.0749457051697354 3053 NA FT TRANSCRIPTION FROM RNA POL II|RESPONSE TO DNA DAMAGE|PROTEIN PHOSPHORYLATION NUCLEUS RNA POL II, HOLOENZYME 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress regulation of transposition, RNA-mediated|phosphorylation of RNA polymerase II C-terminal domain|nucleotide-excision repair, DNA incision|regulation of mitotic recombination|transcription from RNA polymerase II promoter damaged DNA binding|ATP-dependent 5'-3' DNA helicase activity|core RNA polymerase binding transcription factor activity core TFIIH complex|holo TFIIH complex|nucleotide-excision repair factor 3 complex YBR089W_d YBR089W YBR089W het Dubious -1.28727010481714 0.0990000798406624 no 969 0.0713462675738434 3071 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Unknown Unknown Unknown YLR379W_d YLR379W YLR379W het Dubious -1.28877800473085 0.0987376374162147 no 970 0.0956084650732513 3025 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Unknown Unknown Unknown YKL082C YKL082C RRP14 het Verified -1.29183242488472 0.0982075918362228 no 971 0.0792630027627103 3087 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS RIBOSOME Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family ribosomal small subunit biogenesis|ribosomal large subunit biogenesis molecular_function cytosolic large ribosomal subunit|nucleolus YNL149C YNL149C PGA2 het Verified -1.29292422814723 0.0980186334885288 no 972 0.0596170094063461 3130 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect protein transport molecular_function nuclear envelope|endoplasmic reticulum YIL019W YIL019W FAF1 het Verified -1.29584153053741 0.0975150433014557 no 973 0.0943934461479008 3038 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Protein required for pre-rRNA processing and 40S ribosomal subunit assembly maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleolus|nucleus|cytoplasm YBL035C YBL035C POL12 het Verified -1.31452717463772 0.0943344296896792 no 974 0.0840011174923162 3090 NA FT ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation telomere capping|lagging strand elongation|DNA replication initiation DNA-directed DNA polymerase activity nuclear envelope|alpha DNA polymerase:primase complex|nucleus YKL022C YKL022C CDC16 het Verified -1.3216176932289 0.0931477443133074 no 975 0.057256490641934 3112 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|negative regulation of DNA-dependent DNA replication initiation|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YER038C YER038C KRE29 het Verified -1.3249508952348 0.092593719299441 no 976 0.0821803182162265 3079 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance DNA repair molecular_function Smc5-Smc6 complex|nucleus|cytoplasm YMR288W YMR288W HSH155 het Verified -1.32619105229489 0.0923882111635192 no 977 0.0812477860722837 3055 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING NUCLEUS U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 spliceosomal complex assembly|mRNA splicing, via spliceosome mRNA binding U2 snRNP|U2-type prespliceosome YMR076C YMR076C PDS5 het Verified -1.3317662958157 0.0914684982440456 no 978 0.0950663154774349 3015 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis double-strand break repair|meiosis|synapsis|mitotic chromosome condensation|mitotic sister chromatid cohesion structural molecule activity condensed nuclear chromosome YDL165W YDL165W CDC36 het Verified -1.34300698042376 0.0896348567824587 no 979 0.0902836446693858 3065 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|RNA PROCESSING NUCLEUS Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor response to pheromone involved in conjugation with cellular fusion|regulation of cell cycle|deadenylation-independent decapping of nuclear-transcribed mRNA|positive regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay|negative regulation of transcription from RNA polymerase II promoter|nuclear-transcribed mRNA poly(A) tail shortening|transcription elongation from RNA polymerase II promoter|protein ubiquitination ubiquitin-protein ligase activity CCR4-NOT core complex|nucleus|cytoplasm YLR007W YLR007W NSE1 het Verified -1.35226753297316 0.0881448733323157 no 980 0.0700196418111394 3117 NA FT RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair DNA repair|postreplication repair molecular_function Smc5-Smc6 complex|nucleus YPR144C YPR144C NOC4 het Verified -1.3558792342022 0.0875687978195501 no 981 0.0973245772290983 3053 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits ribosomal small subunit biogenesis|endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|rRNA processing molecular_function small-subunit processome|nucleolus|nucleus|90S preribosome|Noc4p-Nop14p complex YIR011C YIR011C STS1 het Verified -1.36642641899548 0.0859025806473697 no 982 0.0839304171831151 3060 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS|CHROMOSOME SEGREGATION NUCLEUS Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation proteasome localization|chromosome segregation|nucleus-associated proteasomal ubiquitin-dependent protein catabolic process molecular_function nucleus YNL256W YNL256W FOL1 het Verified -1.37317015874643 0.0848497271638274 no 983 0.0574090568450967 3138 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|COFACTOR METABOLISM MITOCHONDRION Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities tetrahydrofolate biosynthetic process dihydroneopterin aldolase activity|2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity|dihydropteroate synthase activity mitochondrial envelope|mitochondrion|cytoplasm YEL019C YEL019C MMS21 het Verified -1.39258736649796 0.0818723027583998 no 984 0.0699608604303073 3092 NA FT RESPONSE TO DNA DAMAGE ENDOMEMBRANE SYSTEM|NUCLEUS|CHROMOSOME SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination DNA repair SUMO ligase activity nuclear envelope|Smc5-Smc6 complex|nucleus|cytoplasm YDR396W_d YDR396W YDR396W het Dubious -1.40129934130012 0.0805622890494695 no 985 0.0794558083522954 3079 NA Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Unknown Unknown Unknown YDR499W YDR499W LCD1 het Verified -1.42669688896123 0.0768336413858626 no 986 0.0423143301373745 3196 NA FT PROTEIN LOCALIZATION|CELL CYCLE|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress establishment of protein localization|telomere maintenance via telomerase|DNA damage checkpoint damaged DNA binding nuclear chromosome YKL111C_d YKL111C YKL111C het Dubious -1.42849868833614 0.0765741859436827 no 987 0.0651185555022425 3111 NA Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1 Unknown Unknown Unknown YGL145W YGL145W TIP20 het Verified -1.44152868164491 0.0747176902042403 no 988 0.0576328683998581 3099 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p retrograde vesicle-mediated transport, Golgi to ER molecular_function Dsl1p complex|endoplasmic reticulum YIL048W YIL048W NEO1 het Verified -1.44825438520515 0.0737729598353651 no 989 0.0939600646935312 3077 NA FF|FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|ION HOMEOSTASIS|ION HOMEOSTASIS ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus retrograde vesicle-mediated transport, Golgi to ER|vacuole organization|endocytosis phospholipid-translocating ATPase activity|ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism endosome|COPI-coated vesicle|integral to membrane|Golgi apparatus|Golgi membrane YDR240C YDR240C SNU56 het Verified -1.44896270329576 0.0736739992734927 no 990 0.0949628060722301 3050 NA FT RNA PROCESSING NUCLEUS Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex mRNA splicing, via spliceosome mRNA binding commitment complex|U1 snRNP|U2-type prespliceosome YOR232W YOR232W MGE1 het Verified -1.46645674487534 0.0712619484903341 no 991 0.095111014887773 3093 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE protein refolding|protein import into mitochondrial matrix adenyl-nucleotide exchange factor activity mitochondrion|presequence translocase-associated import motor|mitochondrial matrix YGL048C YGL048C RPT6 het Verified -1.46685183683248 0.0712081824197176 no 992 0.082541552643725 3116 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|PROTEOLYSIS NUCLEUS ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress proteasomal ubiquitin-dependent protein catabolic process|positive regulation of transcription elongation from RNA polymerase II promoter|positive regulation of sequence-specific DNA binding transcription factor activity|nucleotide-excision repair|proteasome regulatory particle assembly|positive regulation of RNA polymerase II transcriptional preinitiation complex assembly|negative regulation of sequence-specific DNA binding transcription factor activity|chromatin remodeling ATPase activity|protein domain specific binding proteasome regulatory particle, base subcomplex|proteasome storage granule|nucleus YFR003C YFR003C YPI1 het Verified -1.47190508460883 0.0705232551827724 no 993 0.0746639109734549 3047 NA FT CELL CYCLE|LIPID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|ION HOMEOSTASIS|GEN. OF PRECURSOR METABOLITES AND ENERGY NUCLEUS Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance negative regulation of phosphoprotein phosphatase activity|glycogen metabolic process|cellular ion homeostasis|Unknown|positive regulation of phosphoprotein phosphatase activity|negative regulation of protein dephosphorylation|mitotic cell cycle spindle assembly checkpoint protein serine/threonine phosphatase inhibitor activity|protein phosphatase type 1 activator activity nucleus YLR305C YLR305C STT4 het Verified -1.47695185453454 0.069844271731102 no 994 0.0496294967892474 3139 NA FT LIPID METABOLISM|SIGNALING MITOCHONDRION|PLASMA MEMBRANE Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization phosphatidylinositol phosphorylation 1-phosphatidylinositol 4-kinase activity mitochondrion|plasma membrane YLR078C YLR078C BOS1 het Verified -1.49085106054206 0.0680003012982732 no 995 0.0647544800237015 3150 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi ER to Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|ER to Golgi transport vesicle membrane|endoplasmic reticulum membrane YLR229C YLR229C CDC42 het Verified -1.4982361261908 0.067035956332997 no 996 0.0853593085230299 3074 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|SIGNALING|CELL DIVISION SITE OF POLARIZED GROWTH|PLASMA MEMBRANE Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins budding cell apical bud growth|Rho protein signal transduction|budding cell isotropic bud growth|regulation of initiation of mating projection growth|regulation of vacuole fusion, non-autophagic|regulation of exit from mitosis|pheromone-dependent signal transduction involved in conjugation with cellular fusion|pseudohyphal growth|establishment of cell polarity|invasive growth in response to glucose limitation|positive regulation of exocytosis GTPase activity|signal transducer activity nuclear membrane|fungal-type vacuole membrane|incipient cellular bud site|plasma membrane|cellular bud neck|mating projection tip|cellular bud tip YER009W YER009W NTF2 het Verified -1.50922756305971 0.0656203193586707 no 997 0.0606436748355202 3171 NA FT PROTEIN LOCALIZATION ENDOMEMBRANE SYSTEM|NUCLEUS Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport nucleocytoplasmic transport|protein import into nucleus Ran GTPase binding nuclear envelope|integral to membrane YKL203C YKL203C TOR2 het Verified -1.51831344186168 0.0644677003286893 no 998 0.0374088760498011 3189 NA FT VESICLE-MEDIATED TRANSPORT|PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|MEMBRANE ORGANIZATION|RIBOSOME BIOGENESIS|SIGNALING|CYTOSKELETON ORGANIZATION MITOCHONDRION|PLASMA MEMBRANE PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication G1 phase of mitotic cell cycle|regulation of cell cycle|actin filament reorganization involved in cell cycle|positive regulation of endocytosis|regulation of cell growth|establishment or maintenance of actin cytoskeleton polarity|Rho protein signal transduction|ribosome biogenesis|signal transduction|TOR signaling cascade|cytoskeleton organization|negative regulation of autophagy protein binding|protein serine/threonine kinase activity TORC1 complex|TORC2 complex|mitochondrion|fungal-type vacuole membrane|extrinsic to internal side of plasma membrane|plasma membrane|cytoplasm YBR155W YBR155W CNS1 het Verified -1.53202613493687 0.062757992798572 no 999 0.0417206875841835 3206 NA TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion protein folding Hsp70 protein binding|Hsp90 protein binding cytoplasm YJL097W YJL097W PHS1 het Verified -1.55860677398978 0.0595447393829361 no 1000 0.0465800697886156 3189 NA FT KETONE METABOLISM|LIPID METABOLISM|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking vacuolar transport|sphingolipid biosynthetic process enoyl-CoA hydratase activity fungal-type vacuole membrane|integral to endoplasmic reticulum membrane|integral to membrane|endoplasmic reticulum|fungal-type vacuole|nuclear outer membrane-endoplasmic reticulum membrane network YFL037W YFL037W TUB2 het Verified -1.55992564166205 0.0593887270844641 no 1001 0.0588397634531553 3137 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CHROMOSOME SEGREGATION NUCLEUS|CYTOSKELETON Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria nuclear migration along microtubule|nuclear migration involved in conjugation with cellular fusion|homologous chromosome segregation|mitotic sister chromatid segregation structural constituent of cytoskeleton tubulin complex|kinetochore microtubule|cytoplasmic microtubule|nuclear microtubule|spindle pole body|polar microtubule YJL054W YJL054W TIM54 het Verified -1.56571242703494 0.0587079778374917 no 1002 0.0543191524595629 3153 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane protein import into mitochondrial inner membrane protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YFR028C YFR028C CDC14 het Verified -1.57690167063405 0.0574090760442501 no 1003 0.0560564981998375 3136 NA FT CELL CYCLE NUCLEUS|CYTOSKELETON Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis regulation of exit from mitosis|mitotic cell cycle|protein dephosphorylation phosphoprotein phosphatase activity nucleolus|RENT complex|nucleus|spindle pole body YLR010C YLR010C TEN1 het Verified -1.58741421779862 0.0562094306356778 no 1004 0.0645175632806041 3133 NA FT NUCLEUS|CHROMOSOME Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p negative regulation of telomere maintenance|telomere capping|telomere maintenance via telomerase single-stranded telomeric DNA binding nuclear telomere cap complex YDR454C YDR454C GUK1 het Verified -1.59121264360273 0.0557808641901471 no 1005 0.0213285628858157 3230 NA FT NUCLEOTIDE METABOLISM NUCLEUS Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins GMP metabolic process guanylate kinase activity nucleus|cytoplasm YGL137W YGL137W SEC27 het Verified -1.59904605663208 0.0549051846702703 no 1006 0.0696732233092655 3128 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|VACUOLAR TRANSPORT ENDOMEMBRANE SYSTEM|GOLGI APPARATUS Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP retrograde vesicle-mediated transport, Golgi to ER|late endosome to vacuole transport via multivesicular body sorting pathway|ER to Golgi vesicle-mediated transport ubiquitin binding COPI vesicle coat YGR198W YGR198W YPP1 het Verified -1.60420771837773 0.0543341369888273 no 1007 0.0387297863935051 3170 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION|VACUOLAR TRANSPORT PLASMA MEMBRANE|CYTOSKELETON Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene receptor-mediated endocytosis|response to pheromone|protein targeting to vacuole molecular_function endosome|cytosol|ribosome|plasma membrane|actin cortical patch|cytoplasm YOL135C YOL135C MED7 het Verified -1.61621377493132 0.0530240507791656 no 1008 0.0532105946602444 3185 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation negative regulation of transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter molecular_function core mediator complex YDR208W YDR208W MSS4 het Verified -1.62983182755127 0.0515685225210858 no 1009 0.0314418558500731 3233 NA FT LIPID METABOLISM NUCLEUS|PLASMA MEMBRANE Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation phosphatidylinositol phosphorylation|ascospore-type prospore assembly 1-phosphatidylinositol-4-phosphate 5-kinase activity nucleus|plasma membrane YJR064W YJR064W CCT5 het Verified -1.63119141258154 0.0514249683690206 no 1010 0.0942341295480522 3012 NA Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo protein folding unfolded protein binding chaperonin-containing T-complex YGR264C YGR264C MES1 het Verified -1.65487577205765 0.0489748498746341 no 1011 0.0550054403022224 3151 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs methionyl-tRNA aminoacylation methionine-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|cytoplasm YFR004W YFR004W RPN11 het Verified -1.65832740004442 0.0486257007213599 no 1012 0.0653917322768649 3092 NA FT PROTEOLYSIS|MITOCHONDRION ORGANIZATION|PEROXISOME ORGANIZATION NUCLEUS|MITOCHONDRION Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress protein deubiquitination|proteasomal ubiquitin-dependent protein catabolic process|peroxisome fission|mitochondrial fission metallopeptidase activity|ubiquitin-specific protease activity cytosol|mitochondrion|proteasome storage granule|nucleus|proteasome regulatory particle, lid subcomplex YOR256C YOR256C TRE2 het Verified -1.66511842565928 0.0479445656775169 no 1013 0.0663487381383925 3114 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|PROTEOLYSIS|VACUOLAR TRANSPORT Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway molecular_function cellular_component YLL003W YLL003W SFI1 het Verified -1.66547435340621 0.0479090780826216 no 1014 0.035545922055624 3206 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION CYTOSKELETON Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C G2/M transition of mitotic cell cycle|spindle assembly|spindle pole body duplication associated with nuclear envelope molecular_function half bridge of spindle pole body YPL063W YPL063W TIM50 het Verified -1.66851823887142 0.0476064471800233 no 1015 0.0477411720061322 3174 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel protein import into mitochondrial matrix mitochondrion targeting sequence binding|protein channel activity mitochondrion|mitochondrial inner membrane presequence translocase complex YDL217C YDL217C TIM22 het Verified -1.67769590267756 0.0467032398664485 no 1016 0.0398786123882426 3224 NA FT PROTEIN LOCALIZATION|MEMBRANE ORGANIZATION|MITOCHONDRION ORGANIZATION MITOCHONDRION Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported protein import into mitochondrial inner membrane mitochondrion targeting sequence binding|protein channel activity mitochondrion|integral to membrane|mitochondrial inner membrane protein insertion complex YPL082C YPL082C MOT1 het Verified -1.68128281347224 0.0463539972701525 no 1017 0.0330888980081336 3203 NA FT TRANSCRIPTION FROM RNA POL II|PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS|MITOCHONDRION|CHROMOSOME Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA regulation of RNA polymerase II transcriptional preinitiation complex assembly|transcription of nuclear large rRNA transcript from RNA polymerase I promoter|regulation of transcription from RNA polymerase II promoter|rRNA processing TBP-class protein binding|ATPase activity nuclear chromosome|mitochondrion YOR103C YOR103C OST2 het Verified -1.68647789566542 0.0458518945444778 no 1018 0.0525104940409645 3209 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YJL005W YJL005W CYR1 het Verified -1.68818707696183 0.0456876615705065 no 1019 0.0321893658228392 3209 NA FT SIGNALING|NUCLEOTIDE METABOLISM MITOCHONDRION|PLASMA MEMBRANE Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation adenylate cyclase-modulating G-protein coupled receptor signaling pathway|Ras protein signal transduction adenylate cyclase activity mitochondrion|plasma membrane YOR148C YOR148C SPP2 het Verified -1.69591363381784 0.0449511186469374 no 1020 0.0412465659425426 3217 NA FT RNA PROCESSING NUCLEUS Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction mRNA splicing, via spliceosome molecular_function spliceosomal complex YPL233W YPL233W NSL1 het Verified -1.69770800657861 0.0447814429343865 no 1021 0.0512770869393078 3149 NA FT CELL CYCLE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME|CYTOSKELETON KINETOCHORE Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation chromosome segregation molecular_function nuclear MIS12/MIND type complex|kinetochore|spindle pole YGR103W YGR103W NOP7 het Verified -1.74503559233962 0.0404893368175462 no 1022 0.0273224597869848 3245 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation ribosomal large subunit biogenesis|maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|cell cycle molecular_function PeBoW complex|nucleolus|nucleus|preribosome, large subunit precursor YEL034W YEL034W HYP2 het Verified -1.75070266037681 0.0399985704732808 no 1023 0.0802183240377294 3134 NA FF|FT KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION RIBOSOME Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication positive regulation of translational initiation|translational frameshifting|positive regulation of translational termination|positive regulation of translational elongation ribosome binding|RNA binding|translation elongation factor activity|translation initiation factor activity mitochondrion|cytosolic ribosome|cytoplasm YKL035W YKL035W UGP1 het Verified -1.76628806676017 0.0386737662943702 no 1024 0.0364121384794017 3224 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication (1->6)-beta-D-glucan biosynthetic process|UDP-glucose metabolic process|trehalose biosynthetic process|glycogen biosynthetic process UTP:glucose-1-phosphate uridylyltransferase activity plasma membrane|cytoplasm YHR070W YHR070W TRM5 het Verified -1.8121790369045 0.0349792685414324 no 1025 0.0376131149359421 3199 NA FT RNA PROCESSING NUCLEUS|MITOCHONDRION tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya tRNA methylation tRNA (guanine) methyltransferase activity nucleus|mitochondrial matrix|cytoplasm YBR079C YBR079C RPG1 het Verified -1.81280362835138 0.0349310570772195 no 1026 0.0227545808966695 3244 NA FT TRANSLATION eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation translation reinitiation|translational initiation translation initiation factor activity incipient cellular bud site|eukaryotic translation initiation factor 3 complex|cytoplasmic stress granule|cytoplasm YPR136C_d YPR136C YPR136C het Dubious -1.81580397888033 0.0347002233664989 no 1027 0.0389406655950829 3163 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Unknown Unknown Unknown YLR212C YLR212C TUB4 het Verified -1.83629407583673 0.0331570877326796 no 1028 0.0104444958824104 3293 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|CELL CYCLE|NUCLEOTIDE METABOLISM|CYTOSKELETON ORGANIZATION NUCLEUS|CYTOSKELETON Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress microtubule nucleation|mitotic spindle organization in nucleus structural constituent of cytoskeleton outer plaque of spindle pole body|gamma-tubulin small complex, spindle pole body|inner plaque of spindle pole body YMR296C YMR296C LCB1 het Verified -1.83989758349358 0.0328916374368635 no 1029 0.0235640783192873 3248 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine sphingolipid biosynthetic process serine C-palmitoyltransferase activity SPOTS complex|serine C-palmitoyltransferase complex YDR023W YDR023W SES1 het Verified -1.84346671793201 0.0326304483657823 no 1030 0.013251006355436 3276 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p seryl-tRNA aminoacylation serine-tRNA ligase activity cytoplasm YMR236W YMR236W TAF9 het Verified -1.85136410708521 0.0320585945562436 no 1031 0.0264503917058709 3270 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION NUCLEUS RNA POL II, HOLOENZYME|HISTONE ACETYLTRANSFERASE Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 histone acetylation|RNA polymerase II transcriptional preinitiation complex assembly|chromatin modification|transcription from RNA polymerase II promoter chromatin binding|protein complex scaffold|RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly SLIK (SAGA-like) complex|SAGA complex|transcription factor TFIID complex YOR204W YOR204W DED1 het Verified -1.86251804208794 0.031265052364592 no 1032 0.0440146841124003 3202 NA FT TRANSLATION ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility translational initiation RNA strand annealing activity|ATP-dependent RNA helicase activity cytoplasm YIR022W YIR022W SEC11 het Verified -1.93731702218735 0.0263533013691373 no 1033 0.0220807076555561 3258 NA FT PROTEIN LOCALIZATION|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum signal peptide processing|protein targeting to ER peptidase activity signal peptidase complex|endoplasmic reticulum YLR259C YLR259C HSP60 het Verified -1.94461747661212 0.0259105171524956 no 1034 0.013018372819333 3293 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|MITOCHONDRION ORGANIZATION MITOCHONDRION Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated protein refolding|protein stabilization|chaperone-mediated protein complex assembly|'de novo' protein folding|protein maturation|protein import into mitochondrial intermembrane space unfolded protein binding|DNA replication origin binding|single-stranded DNA binding|ATPase activity|chaperone binding mitochondrial nucleoid|mitochondrion YNL244C YNL244C SUI1 het Verified -1.95454424175553 0.0253184440321442 no 1035 0.0222074795877864 3255 NA FT TRANSLATION Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase translational initiation translation initiation factor binding|translation initiation factor activity multi-eIF complex YDL064W YDL064W UBC9 het Verified -1.96204968133908 0.0248783502082045 no 1036 0.0154037930910508 3256 NA FT CELL CYCLE|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|CHROMOSOME SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) G2/M transition of mitotic cell cycle|protein sumoylation|mitotic spindle elongation SUMO ligase activity condensed nuclear chromosome|nucleus YJR016C YJR016C ILV3 het Verified -1.96330334757521 0.02480546835872 no 1037 0.0162954936864005 3284 NA FT KETONE METABOLISM|AMINO ACID METABOLISM MITOCHONDRION Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids branched-chain amino acid biosynthetic process dihydroxy-acid dehydratase activity mitochondrion YER036C YER036C ARB1 het Verified -1.98209308657022 0.0237344115886334 no 1038 0.0128485629412362 3274 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS|NUCLEOTIDE METABOLISM NUCLEUS ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p ribosomal small subunit export from nucleus|ribosome biogenesis ATPase activity, uncoupled ribosome|nucleus|cytoplasm YLR103C YLR103C CDC45 het Verified -1.99575292945882 0.0229804113289501 no 1039 0.0124896801844182 3277 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes S phase of mitotic cell cycle|regulation of chromatin silencing at telomere|DNA replication initiation|pre-replicative complex assembly|double-strand break repair via break-induced replication DNA replication origin binding|chromatin binding replication fork protection complex|nucleus|DNA replication preinitiation complex|nuclear replication fork|pre-replicative complex YKR068C YKR068C BET3 het Verified -2.03079580536935 0.0211378563655482 no 1040 0.0181386363071742 3284 NA FT VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM|GOLGI APPARATUS Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport Rab guanyl-nucleotide exchange factor activity TRAPP complex|cis-Golgi network membrane YBL020W YBL020W RFT1 het Verified -2.0425591107137 0.0205480511544276 no 1041 0.0342952508570885 3234 NA FT MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein glycolipid translocation molecular_function|glycolipid-translocating activity integral to membrane|endoplasmic reticulum membrane YDR236C YDR236C FMN1 het Verified -2.05181064178186 0.0200940343436377 no 1042 0.0117665039602528 3271 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM ENDOPLASMIC RETICULUM|MITOCHONDRION Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane FMN biosynthetic process riboflavin kinase activity|FMN adenylyltransferase activity intracellular membrane-bounded organelle|mitochondrial inner membrane YKL196C YKL196C YKT6 het Verified -2.0641521993091 0.0195016440232372 no 1043 0.0105081664309291 3276 NA FT VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION MITOCHONDRION|GOLGI APPARATUS|PLASMA MEMBRANE Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus intra-Golgi vesicle-mediated transport|vacuole fusion, non-autophagic|vesicle fusion palmitoyltransferase activity|SNAP receptor activity SNARE complex|endosome|mitochondrion|Golgi apparatus|fungal-type vacuole|membrane YML105C YML105C SEC65 het Verified -2.11409149656247 0.0172537266661554 no 1044 0.0137576648068317 3281 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER 7S RNA binding signal recognition particle, endoplasmic reticulum targeting YGL091C YGL091C NBP35 het Verified -2.19685772873425 0.0140153043108806 no 1045 0.00452234623084209 3316 NA FT RNA PROCESSING|COFACTOR METABOLISM NUCLEUS Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases iron-sulfur cluster assembly|tRNA wobble uridine modification 4 iron, 4 sulfur cluster binding|ATPase activity|iron ion binding mitochondrion|nucleus|cytoplasm YMR131C YMR131C RRB1 het Verified -2.21211772139681 0.0134792659777133 no 1046 0.0134109514357943 3277 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p ribosome biogenesis molecular_function nucleolus YOR149C YOR149C SMP3 het Verified -2.23388251382524 0.0127454022018232 no 1047 0.00568888534000629 3328 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure GPI anchor biosynthetic process|plasmid maintenance dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity integral to membrane|endoplasmic reticulum YDR361C YDR361C BCP1 het Verified -2.23497573800688 0.0127094708048743 no 1048 0.0110139871118538 3284 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|RIBOSOME BIOGENESIS NUCLEUS Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport protein export from nucleus|ribosomal large subunit export from nucleus molecular_function nucleus|cytoplasm YBL004W YBL004W UTP20 het Verified -2.24108336661607 0.0125103377100081 no 1049 0.011131953247004 3291 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)|endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) molecular_function nucleoplasm|small-subunit processome|preribosome, small subunit precursor|nucleolus|90S preribosome|cytoplasm YGL245W YGL245W GUS1 het Verified -2.24818471916076 0.012282206829137 no 1050 0.0121011707831155 3324 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION MITOCHONDRION Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress glutamyl-tRNA aminoacylation glutamate-tRNA ligase activity methionyl glutamyl tRNA synthetase complex|mitochondrion|cytoplasm YDR331W YDR331W GPI8 het Verified -2.29064746444381 0.0109919056941948 no 1051 0.0127279732211375 3288 NA FT LIPID METABOLISM|PROTEOLYSIS ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog attachment of GPI anchor to protein GPI-anchor transamidase activity endoplasmic reticulum membrane|GPI-anchor transamidase complex YKL122C YKL122C SRP21 het Verified -2.36828354394757 0.00893541704451521 no 1052 0.00462737029444484 3304 NA FT PROTEIN LOCALIZATION|PROTEIN COMPLEX ORG/BIOGENESIS|TRANSLATION NUCLEUS Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition|protein targeting to ER signal sequence binding nucleus|signal recognition particle, endoplasmic reticulum targeting YMR308C YMR308C PSE1 het Verified -2.40181492166469 0.0081569800197534 no 1053 0.00480718112178444 3311 NA FT PROTEIN LOCALIZATION|CELL CYCLE|RNA LOCALIZATION NUCLEUS Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p mRNA export from nucleus|regulation of protein desumoylation|protein import into nucleus|regulation of mitosis protein transporter activity|nuclear localization sequence binding integral to membrane|nucleus|cytoplasm YOR020C YOR020C HSP10 het Verified -2.42595511071312 0.0076340793343225 no 1054 0.0088528444714871 3304 NA FT MITOCHONDRION Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES protein folding|protein refolding unfolded protein binding|chaperone binding mitochondrion|mitochondrial matrix YDL045C YDL045C FAD1 het Verified -2.43242175503249 0.00749911583242472 no 1055 0.0046188933270408 3334 NA FT NUCLEOTIDE METABOLISM|COFACTOR METABOLISM Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin FAD biosynthetic process FMN adenylyltransferase activity cytoplasm YDL007W YDL007W RPT2 het Verified -2.44402744769659 0.00726216074502064 no 1056 0.00649927282600047 3303 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS NUCLEUS One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle positive regulation of protein catabolic process|proteasome regulatory particle assembly|peptide catabolic process|ubiquitin-dependent protein catabolic process ATPase activity proteasome regulatory particle, base subcomplex|nucleus YMR149W YMR149W SWP1 het Verified -2.45564365015727 0.00703162667847519 no 1057 0.00248420249696681 3334 NA FT CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum protein N-linked glycosylation dolichyl-diphosphooligosaccharide-protein glycotransferase activity oligosaccharyltransferase complex|integral to membrane YDR303C YDR303C RSC3 het Verified -2.49795241365748 0.00624564802790112 no 1058 0.00865556003231525 3273 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|CHROMATIN ORGANIZATION NUCLEUS RSC COMPLEX Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p nucleosome positioning|ATP-dependent chromatin remodeling|regulation of cell cycle|nucleosome disassembly|regulation of DNA replication involved in S phase|regulation of transcription, DNA-dependent|G1/S transition of mitotic cell cycle|G2/M transition of mitotic cell cycle|transcription elongation from RNA polymerase II promoter sequence-specific DNA binding|DNA translocase activity|DNA binding RSC complex|nucleus YBL018C YBL018C POP8 het Verified -2.50800699333469 0.0060707132083709 no 1059 0.00338554641129427 3311 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs tRNA processing|intronic box C/D snoRNA processing|mRNA cleavage|rRNA processing ribonuclease MRP activity|ribonuclease P activity nucleolar ribonuclease P complex|ribonuclease MRP complex YGL073W YGL073W HSF1 het Verified -2.56128813270998 0.00521424133429428 no 1060 0.00220801086678175 3321 NA FT TRANSCRIPTION FROM RNA POL II|CELL CYCLE|SIGNALING|CYTOSKELETON ORGANIZATION NUCLEUS|MITOCHONDRION Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated spindle pole body duplication|negative regulation of TOR signaling cascade|response to heat|regulation of transcription from RNA polymerase II promoter|response to stress sequence-specific DNA binding|sequence-specific DNA binding transcription factor activity mitochondrion|nucleus YOR218C_d YOR218C YOR218C het Dubious -2.56976034440665 0.00508844455698949 no 1061 0.00154502075297298 3316 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Unknown Unknown Unknown YJL032W_d YJL032W YJL032W het Dubious -2.57480180133871 0.00501487709399995 no 1062 0.00217508050760369 3327 NA Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase Unknown Unknown Unknown YIR006C YIR006C PAN1 het Verified -2.57730998256888 0.00497863054688066 no 1063 0.0017784869275366 3338 NA FT VESICLE-MEDIATED TRANSPORT|CELL CYCLE|MEMBRANE ORGANIZATION|CELL DIVISION|CYTOSKELETON ORGANIZATION NUCLEUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE|CYTOSKELETON Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease bipolar cellular bud site selection|endocytosis|axial cellular bud site selection|cytokinesis|actin cortical patch assembly protein binding, bridging nucleus|plasma membrane|mating projection tip|cytoplasm|actin cortical patch YDL055C YDL055C PSA1 het Verified -2.60670461807114 0.00457090923836138 no 1064 0.00328155623190787 3327 NA FT CELL CYCLE|CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure protein glycosylation|cell wall mannoprotein biosynthetic process|GDP-mannose biosynthetic process mannose-1-phosphate guanylyltransferase activity cytoplasm YMR043W YMR043W MCM1 het Verified -2.61894004269901 0.00441017244092534 no 1065 0.00470495333273111 3326 NA FT TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|CELL CYCLE|AMINO ACID METABOLISM NUCLEUS|CHROMOSOME Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes regulation of mating type switching|positive regulation of transcription involved in G2/M-phase of mitotic cell cycle|DNA replication initiation|negative regulation of mating-type specific transcription from RNA polymerase II promoter|negative regulation of transcription from RNA polymerase II promoter|Unknown|positive regulation of mating-type specific transcription from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter DNA replication origin binding|RNA polymerase II activating transcription factor binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription|RNA polymerase II core promoter proximal region sequence-specific DNA binding, bending|RNA polymerase II core promoter proximal region sequence-specific DNA binding|sequence-specific DNA binding|RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription|sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity|RNA polymerase II repressing transcription factor binding nuclear chromatin YJR045C YJR045C SSC1 het Verified -2.62702708724972 0.00430672385646029 no 1066 0.0070231387865628 3305 NA FT PROTEIN LOCALIZATION|MITOCHONDRION ORGANIZATION NUCLEUS|MITOCHONDRION Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication protein refolding|positive regulation of endodeoxyribonuclease activity|protein import into mitochondrial matrix|protein unfolding enzyme regulator activity|ATPase activity mitochondrial nucleoid|mitochondrion|TRC complex|mitochondrial inner membrane|presequence translocase-associated import motor YPL044C_d YPL044C YPL044C het Dubious -2.6951897373824 0.00351742797511447 no 1067 0.00158905827863336 3339 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Unknown Unknown Unknown YHR058C YHR058C MED6 het Verified -2.71485870170533 0.00331520265229319 no 1068 0.00568652198522747 3307 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress RNA polymerase II transcriptional preinitiation complex assembly|positive regulation of transcription from RNA polymerase II promoter RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly core mediator complex YBR243C YBR243C ALG7 het Verified -2.73473988967938 0.00312147934223929 no 1069 0.00120769171439404 3327 NA FT OXIDATION-REDUCTION PROCESS|CARBOHYDRATE METABOLISM|GEN. OF PRECURSOR METABOLITES AND ENERGY ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin protein N-linked glycosylation|aerobic respiration UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity UDP-N-acetylglucosamine transferase complex|integral to membrane YPR103W YPR103W PRE2 het Verified -2.80801167519108 0.00249242097743814 no 1070 0.0040311542947026 3296 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|PROTEOLYSIS ENDOMEMBRANE SYSTEM|NUCLEUS|ENDOPLASMIC RETICULUM Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome proteasomal ubiquitin-dependent protein catabolic process|proteasome core complex assembly|proteasomal ubiquitin-independent protein catabolic process endopeptidase activity nucleus|proteasome storage granule|endoplasmic reticulum membrane|proteasome core complex, beta-subunit complex YMR200W YMR200W ROT1 het Verified -2.83207754217011 0.00231233160397192 no 1071 0.00108838962223489 3340 NA FT CELL CYCLE|SIGNALING|CELL WALL ORG/BIOGENESIS|CELL DIVISION|CARBOHYDRATE METABOLISM|CYTOSKELETON ORGANIZATION ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation budding cell apical bud growth|cell cycle cytokinesis|establishment or maintenance of actin cytoskeleton polarity|protein O-linked mannosylation|endoplasmic reticulum unfolded protein response|protein folding|fungal-type cell wall biogenesis|'de novo' protein folding|protein N-linked glycosylation unfolded protein binding endoplasmic reticulum|endoplasmic reticulum membrane YOR370C YOR370C MRS6 het Verified -2.84254673401528 0.00223773345509954 no 1072 0.00123102967322163 3338 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|SIGNALING|NUCLEOTIDE METABOLISM|PROTEIN PRENYLATION Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress protein targeting to membrane|protein geranylgeranylation|ER to Golgi vesicle-mediated transport|activation of Rab GTPase activity Rab geranylgeranyltransferase activity|Rab GTPase binding Rab-protein geranylgeranyltransferase complex|membrane|cytoplasm YOR272W YOR272W YTM1 het Verified -2.8897473264023 0.00192775792395184 no 1073 0.00177599030301714 3341 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|RIBOSOME BIOGENESIS NUCLEUS Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats chromosome organization|ribosomal large subunit biogenesis molecular_function PeBoW complex|nucleolus|nucleus|preribosome, large subunit precursor YAL034C-B_d YAL034C-B YAL034C-B het Dubious -2.90155989220996 0.00185654896547044 no 1074 0.00178077155656542 3322 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Unknown Unknown Unknown YOR336W YOR336W KRE5 het Verified -2.97779309969382 0.00145165949949892 no 1075 0.000967601606135821 3349 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOPLASMIC RETICULUM Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects (1->6)-beta-D-glucan biosynthetic process UDP-glucose:glycoprotein glucosyltransferase activity endoplasmic reticulum YDR037W YDR037W KRS1 het Verified -3.30755100963045 0.00047057784937401 yes 1076 1.72118398426669e-05 3345 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Lysyl-tRNA synthetase lysyl-tRNA aminoacylation lysine-tRNA ligase activity cytoplasm YOR194C YOR194C TOA1 het Verified -3.33033758123494 0.000433703757469272 yes 1077 0.000343087051402635 3351 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA transcription initiation from RNA polymerase II promoter|RNA polymerase II transcriptional preinitiation complex assembly TBP-class protein binding|TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly transcription factor TFIIA complex YER022W YER022W SRB4 het Verified -3.36396221793295 0.000384160303030632 yes 1078 0.00018511428475837 3335 NA FT TRANSCRIPTION FROM RNA POL II NUCLEUS RNA POL II, HOLOENZYME Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination transcription initiation from RNA polymerase II promoter|positive regulation of transcription from RNA polymerase II promoter activating transcription factor binding|structural molecule activity|core RNA polymerase II recruiting transcription factor activity core mediator complex YFL008W YFL008W SMC1 het Verified -3.54604119212063 0.000195532536217187 yes 1079 0.000297558996717233 3333 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE|CELL DIVISION|CHROMOSOME SEGREGATION NUCLEUS|CHROMOSOME Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure double-strand break repair|mitotic sister chromatid cohesion|mitotic sister chromatid segregation double-stranded DNA binding|ATPase activity|AT DNA binding|DNA secondary structure binding nuclear mitotic cohesin complex YJL173C YJL173C RFA3 het Verified -3.56431363245457 0.000182404772224906 yes 1080 0.000123270533689131 3347 NA FT PROTEIN LOCALIZATION|CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress establishment of protein localization|telomere maintenance via telomerase|telomere maintenance via recombination|nucleotide-excision repair|DNA repair|mitotic recombination|heteroduplex formation|DNA unwinding involved in replication|reciprocal meiotic recombination|double-strand break repair via homologous recombination|DNA replication|protein ubiquitination double-stranded DNA binding|sequence-specific DNA binding condensed nuclear chromosome|chromosome, telomeric region|DNA replication factor A complex YDL008W YDL008W APC11 het Verified -3.67976045768845 0.000116726571359546 yes 1081 2.27181384614951e-05 3352 NA FT CELL CYCLE|CELL DIVISION|PROTEOLYSIS NUCLEUS UBIQUITIN LIGASE COMPLEX Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|protein ubiquitination ubiquitin-protein ligase activity anaphase-promoting complex YGL150C YGL150C INO80 het Verified -3.7731254467516 8.06075874278249e-05 yes 1082 0.000239646024286571 3347 NA FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RESPONSE TO DNA DAMAGE|GENE SILENCING NUCLEUS|CHROMOSOME ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription nucleosome positioning|histone exchange|transcription from RNA polymerase II promoter|DNA repair|nucleosome mobilization|chromatin remodeling|chromatin silencing at telomere|regulation of transcription from RNA polymerase II promoter in response to stress|positive regulation of transcription from RNA polymerase II promoter ATPase activity|ATP-dependent 3'-5' DNA helicase activity Ino80 complex YPL160W YPL160W CDC60 het Verified -3.7805063107008 7.82548760023102e-05 yes 1083 2.04101771767009e-05 3352 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|TRANSLATION Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA leucyl-tRNA aminoacylation|DNA replication initiation leucine-tRNA ligase activity cytoplasm YLR026C YLR026C SED5 het Verified -3.79552508685823 7.36656476398763e-05 yes 1084 5.76366163407715e-05 3345 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|MEMBRANE ORGANIZATION ENDOMEMBRANE SYSTEM|GOLGI APPARATUS cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins ER to Golgi vesicle-mediated transport|intra-Golgi vesicle-mediated transport|vesicle fusion with Golgi apparatus|vesicle fusion SNAP receptor activity SNARE complex|integral to membrane|cis-Golgi network YKL173W YKL173W SNU114 het Verified -3.83478213760446 6.28378196899172e-05 yes 1085 8.4192883388541e-05 3348 NA FT PROTEIN COMPLEX ORG/BIOGENESIS|RNA PROCESSING|NUCLEOTIDE METABOLISM NUCLEUS GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 assembly of spliceosomal tri-snRNP|spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)|mRNA splicing, via spliceosome|generation of catalytic spliceosome for first transesterification step GTP binding|GTPase activity|U5 snRNA binding U4/U6 x U5 tri-snRNP complex|U5 snRNP YDR473C YDR473C PRP3 het Verified -3.9173013849335 4.47728845571268e-05 yes 1086 0.000332930899903999 3345 NA FT RNA PROCESSING NUCLEUS Splicing factor, component of the U4/U6-U5 snRNP complex mRNA splicing, via spliceosome molecular_function U4/U6 x U5 tri-snRNP complex YER125W YER125W RSP5 het Verified -4.11779505047246 1.9125731874694e-05 yes 1087 7.01217391738174e-05 3348 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT|TRANSCRIPTION FROM RNA POL II|KETONE METABOLISM|PROTEIN COMPLEX ORG/BIOGENESIS|LIPID METABOLISM|MEMBRANE ORGANIZATION|RNA PROCESSING|RIBOSOME BIOGENESIS|CHROMATIN ORGANIZATION|PROTEOLYSIS|MITOCHONDRION ORGAN NUCLEUS|MITOCHONDRION|GOLGI APPARATUS|SITE OF POLARIZED GROWTH|PLASMA MEMBRANE UBIQUITIN LIGASE COMPLEX E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p cellular response to UV|late endosome to vacuole transport via multivesicular body sorting pathway|chromatin assembly or disassembly|response to drug|protein ubiquitination involved in ubiquitin-dependent protein catabolic process|ubiquitin-dependent endocytosis|protein monoubiquitination|positive regulation of fatty acid biosynthetic process|positive regulation of receptor-mediated endocytosis|regulation of phosphate metabolic process|regulation of ubiquinone biosynthetic process|regulation of dolichol biosynthetic process|regulation of multivesicular body size|positive regulation of transcription from RNA polymerase II promoter|regulation of nitrogen utilization|proteasomal ubiquitin-dependent protein catabolic process|positive regulation of endocytosis|regulation of actin cytoskeleton organization|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|regulation of protein localization|mitochondrion organization|regulation of mRNA export from nucleus|regulation of ergosterol biosynthetic process|ribophagy|Unknown|protein polyubiquitination ubiquitin binding|ubiquitin-protein ligase activity|phosphatidylinositol binding mitochondrion|ubiquitin ligase complex|Golgi apparatus|cytoplasm|cellular bud tip|endosome membrane|extrinsic to internal side of plasma membrane|plasma membrane|nucleus YKL189W YKL189W HYM1 het Verified -4.28206914137492 9.25817121202096e-06 yes 1088 2.3435573186609e-08 3355 NA FT CELL DIVISION SITE OF POLARIZED GROWTH Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response cytokinesis, completion of separation|regulation of cell shape|regulation of transcription, DNA-dependent|budding cell apical bud growth molecular_function incipient cellular bud site|intracellular|cellular bud|mating projection tip YDL120W YDL120W YFH1 het Verified -4.33270197170404 7.36452176530214e-06 yes 1089 8.18901746540416e-07 3355 NA FT KETONE METABOLISM|AMINO ACID METABOLISM|ION HOMEOSTASIS|OXIDATION-REDUCTION PROCESS|ION HOMEOSTASIS|COFACTOR METABOLISM MITOCHONDRION Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia cellular iron ion homeostasis|iron-sulfur cluster assembly|glutathione metabolic process ferrous iron binding|ferroxidase activity|iron chaperone activity mitochondrion|mitochondrial matrix YDR434W YDR434W GPI17 het Verified -4.50822481139809 3.26861440767398e-06 yes 1090 3.30504846166895e-08 3355 NA FT LIPID METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog attachment of GPI anchor to protein GPI-anchor transamidase activity integral to membrane|endoplasmic reticulum|GPI-anchor transamidase complex YOR203W_d YOR203W YOR203W het Dubious -4.87162578215326 5.53418311813687e-07 yes 1091 5.57277901768402e-07 3356 NA Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Unknown Unknown Unknown YDR013W YDR013W PSF1 het Verified -5.39268467624773 3.47063498081808e-08 yes 1092 7.95289384813787e-09 3351 NA FT CELL CYCLE|RESPONSE TO DNA DAMAGE NUCLEUS|CHROMOSOME Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery double-strand break repair via break-induced replication|DNA-dependent DNA replication molecular_function GINS complex|replication fork protection complex|DNA replication preinitiation complex YPR133C YPR133C SPN1 het Verified -5.57704609378812 1.22318531945903e-08 yes 1093 5.70834200125829e-06 3348 NA FF|FT TRANSCRIPTION FROM RNA POL II|CHROMATIN ORGANIZATION|RNA LOCALIZATION NUCLEUS Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype poly(A)+ mRNA export from nucleus|regulation of transcription elongation from RNA polymerase II promoter|regulation of transcription by chromatin organization|regulation of transcription from RNA polymerase II promoter core RNA polymerase II binding transcription factor activity|RNA polymerase II transcription factor binding transcription factor activity nucleus YBL040C YBL040C ERD2 het Verified -5.88615670117556 1.97639778643797e-09 yes 1094 4.65801112706764e-11 3350 NA FT PROTEIN LOCALIZATION|VESICLE-MEDIATED TRANSPORT ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins protein retention in ER lumen|ER to Golgi vesicle-mediated transport HDEL sequence binding integral to endoplasmic reticulum membrane|integral to membrane YFR042W YFR042W KEG1 het Verified -7.23118711744113 2.39395054027387e-13 yes 1095 1.58689016713559e-14 3355 NA FT CELL WALL ORG/BIOGENESIS|CARBOHYDRATE METABOLISM ENDOMEMBRANE SYSTEM|ENDOPLASMIC RETICULUM Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability chromosome organization|(1->6)-beta-D-glucan biosynthetic process molecular_function integral to endoplasmic reticulum membrane|integral to membrane