YAL059W / ECM1

Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL059W / ECM1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.09|P-value:8.58E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.68|P-value:4.71E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.64|P-value:1.04E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.94|P-value:2.07E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:3.51|P-value:9.74E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:3.02|P-value:6.51E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:3.28|P-value:2.42E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:3.04|P-value:6.07E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.04|P-value:6.03E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.19|P-value:5.89E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.29|P-value:2.38E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-3.72|P-value:7.46E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.56|P-value:8.01E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.53|P-value:9.15E-5 ID:SGTC_1782|Compound:st039423|FD-Score:-3.55|P-value:1.47E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.01|P-value:6.87E-4 ID:SGTC_2063|Compound:5246145|FD-Score:2.97|P-value:7.67E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.73|P-value:3.89E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.00|P-value:6.98E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.28|P-value:4.27E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:5.75|P-value:6.91E-10 ID:SGTC_2703|Compound:st077852|FD-Score:2.93|P-value:9.01E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-3.49|P-value:1.91E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.13|P-value:7.47E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.58|P-value:7.18E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.09|P-value:8.58E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.68|P-value:4.71E-5 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.64|P-value:1.04E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.94|P-value:2.07E-7 ID:SGTC_358|Compound:0075-0058|FD-Score:3.51|P-value:9.74E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:3.02|P-value:6.51E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:3.28|P-value:2.42E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:3.04|P-value:6.07E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:3.04|P-value:6.03E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.19|P-value:5.89E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.29|P-value:2.38E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:-3.72|P-value:7.46E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.56|P-value:8.01E-5 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.53|P-value:9.15E-5 ID:SGTC_1782|Compound:st039423|FD-Score:-3.55|P-value:1.47E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.01|P-value:6.87E-4 ID:SGTC_2063|Compound:5246145|FD-Score:2.97|P-value:7.67E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.73|P-value:3.89E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.00|P-value:6.98E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.28|P-value:4.27E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:5.75|P-value:6.91E-10 ID:SGTC_2703|Compound:st077852|FD-Score:2.93|P-value:9.01E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-3.49|P-value:1.91E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.13|P-value:7.47E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.58|P-value:7.18E-5

Top fitness defect scores for YAL059W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2697 mebhydrolin 5.75 72.4 µM 6.91E-10 NEO1 33 4.36E-9
2 SGTC_2515 chrysin 3.73 20.8 µM 3.89E-5 RPP1 & pyrimidine depletion 12 9.66E-5
3 SGTC_280 3448-3607 3.68 3.0 µM 4.71E-5 60S ribosome export 14 1.15E-4
4 SGTC_3303 9125618 3.58 40.6 µM 7.18E-5 58 1.69E-4
5 SGTC_1224 0823-0416 3.56 294.0 µM 8.01E-5 21 1.87E-4
6 SGTC_1232 0279-0259 3.53 161.0 µM 9.15E-5 35 2.11E-4
7 SGTC_358 0075-0058 3.51 9.4 µM 9.74E-5 86 2.23E-4
8 SGTC_1220 1838-0075 3.29 193.0 µM 2.38E-4 26 5.05E-4
9 SGTC_645 0845-0943 3.28 5.4 µM 2.42E-4 60S ribosome export 26 5.11E-4
10 SGTC_832 4083-0019 3.04 164.0 µM 6.03E-4 translation 189 0.00118
11 SGTC_662 k832-1615 3.04 104.0 µM 6.07E-4 61 0.00118
12 SGTC_404 fumagillin 3.02 50.0 µM 6.51E-4 61 0.00126
13 SGTC_2008 4012253 3.01 172.0 µM 6.87E-4 75 0.00133
14 SGTC_2518 prenyletin 3.00 46.3 µM 6.98E-4 32 0.00135
15 SGTC_2063 5246145 2.97 200.0 µM 7.67E-4 69 0.00147
16 SGTC_2703 st077852 2.93 53.4 µM 9.01E-4 74 0.00170
17 SGTC_634 1611-4317 2.86 11.2 µM 0.00114 ERG2 65 0.00210
18 SGTC_1508 1080-0625 2.86 72.9 µM 0.00115 55 0.00212
19 SGTC_182 3448-4371 2.85 550.5 µM 0.00119 70 0.00219
20 SGTC_1727 st034309 2.81 9.9 µM 0.00138 127 0.00250

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.228 8.70E-41 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.184 9.68E-27 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.176 8.25E-25 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.173 4.93E-24 YFL001W DEG1 hom tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
0.155 1.32E-19 YLR402W_d YLR402W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.154 2.39E-19 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.147 9.73E-18 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.142 1.16E-16 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.138 8.46E-16 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.135 3.68E-15 YDR378C LSM6 hom Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
0.133 1.15E-14 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.131 2.94E-14 YKR092C SRP40 hom Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
0.125 3.75E-13 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.121 2.14E-12 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.120 2.80E-12 YDR057W YOS9 hom ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family