HIPHOP chemogenomics database

YBR035C / PDX3

Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR035C / PDX3

Click on Significant Values for Screen Details

Top fitness defect scores for YBR035C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_171	k064-0041	15.50	9.7 µM	3.98E-59	heme requiring	1	2.56E-54
2	SGTC_73	0327-0325	13.40	16.2 µM	5.36E-45	DNA damage response	2	2.21E-41
3	SGTC_902	1120-0002	8.68	2.1 µM	6.35E-20	azole & statin	7	2.04E-18
4	SGTC_236	1509-0011	8.56	5.3 µM	1.85E-19		235	5.43E-18
5	SGTC_438	sphingosine	8.22	6.7 µM	4.65E-18		2	1.05E-16
6	SGTC_1676	st018489	7.85	23.4 µM	1.19E-16	60S ribosome export	1	2.05E-15
7	SGTC_547	1120-0019	7.35	610.0 nM	8.52E-15	azole & statin	4	1.02E-13
8	SGTC_227	fluconazole	6.61	33.5 µM	2.48E-12	azole & statin	35	1.86E-11
9	SGTC_1055	3448-0672	5.62	86.0 µM	2.21E-9		13	9.51E-9
10	SGTC_1660	st012960	5.31	28.1 µM	1.45E-8	iron homeostasis	14	5.36E-8
11	SGTC_623	k072-0108	5.28	16.9 µM	1.74E-8	heme biosynthesis & mitochondrial translocase	4	6.33E-8
12	SGTC_660	4031-1742	5.23	102.0 µM	2.43E-8	redox potentiating	17	8.58E-8
13	SGTC_556	4058-0063	5.19	51.5 µM	3.11E-8		13	1.08E-7
14	SGTC_350	k064-0020	5.17	19.8 µM	3.36E-8	heme requiring	9	1.16E-7
15	SGTC_156	3474-0145	5.05	5.6 µM	6.69E-8	endomembrane recycling	32	2.18E-7
16	SGTC_1044	1222-0021	5.05	1.8 µM	6.90E-8	heme biosynthesis & mitochondrial translocase	22	2.24E-7
17	SGTC_2356	9061756	4.69	189.0 µM	4.90E-7		6	1.36E-6
18	SGTC_1290	0986-0249	4.69	10.2 µM	5.05E-7	DNA intercalators	12	1.40E-6
19	SGTC_157	1348-1695	4.43	65.8 µM	1.88E-6	iron homeostasis	47	4.67E-6
20	SGTC_3081	9117243	4.41	49.5 µM	2.12E-6	fatty acid desaturase (OLE1)	14	5.22E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.302	1.26E-71	YDL118W_d	YDL118W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.272	4.55E-58	YDL119C_p	YDL119C_p	hom	Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.230	1.24E-41	YER061C	CEM1	hom	Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
0.212	2.87E-35	YJL046W	AIM22	hom	Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
0.203	1.62E-32	YKL109W	HAP4	hom	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.198	4.93E-31	YHR050W-A_p	YHR050W-A_p	hom	Protein of unknown function; identified by expression profiling and mass spectrometry
0.197	1.36E-30	YDR232W	HEM1	het	5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.193	1.95E-29	YBR227C	MCX1	hom	Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins
0.190	1.08E-28	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.189	2.22E-28	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.189	2.51E-28	YBL021C	HAP3	hom	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
0.180	7.97E-26	YGL237C	HAP2	hom	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.177	4.75E-25	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.168	1.22E-22	YHR045W_p	YHR045W_p	hom	Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
0.164	1.01E-21	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases