Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
Profile for YBR085C-A / YBR085C-A

Click on Significant Values for Screen Details

Top fitness defect scores for YBR085C-A deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1718 roemerine 5.45 11.9 µM 3.53E-10 redox potentiating 17 2.49E-8
2 SGTC_1714 st033236 5.41 13.6 µM 4.72E-10 6 3.13E-8
3 SGTC_236 1509-0011 5.11 5.3 µM 3.77E-9 322 1.60E-7
4 SGTC_1716 st033144 5.06 30.6 µM 5.39E-9 5 2.12E-7
5 SGTC_3285 9112818 4.87 20.9 µM 1.85E-8 cell wall 19 5.58E-7
6 SGTC_1635 st007035 4.54 42.4 µM 1.41E-7 182 2.75E-6
7 SGTC_103 0844-0104 4.17 51.8 µM 1.27E-6 ERAD & cell cycle 135 1.55E-5
8 SGTC_1200 1643-0193 3.98 18.4 µM 3.53E-6 15 3.48E-5
9 SGTC_2977 9011197 3.94 71.4 µM 4.26E-6 18 4.04E-5
10 SGTC_2372 9071633 3.91 200.0 µM 5.07E-6 12 4.63E-5
11 SGTC_2843 9000894 3.86 45.5 µM 6.65E-6 12 5.74E-5
12 SGTC_3217 9129673 3.81 49.5 µM 8.58E-6 45 7.02E-5
13 SGTC_847 0342-0274 3.74 5.5 µM 1.19E-5 23 9.07E-5
14 SGTC_1507 4075-3230 3.65 155.0 µM 1.93E-5 14 1.33E-4
15 SGTC_709 3807-1509 3.56 243.0 µM 2.96E-5 RSC complex & mRNA processing 27 1.87E-4
16 SGTC_3280 9140961 3.55 49.5 µM 3.10E-5 60S ribosome export 26 1.94E-4
17 SGTC_2716 st080858 3.45 38.6 µM 4.91E-5 60S ribosome export 29 2.79E-4
18 SGTC_1247 0448-0043 3.32 5.8 µM 9.03E-5 ERG2 82 4.52E-4
19 SGTC_815 1582-0027 3.31 36.9 µM 9.21E-5 62 4.59E-4
20 SGTC_1505 k297-0037 3.20 20.6 µM 1.53E-4 43 6.87E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.282 1.52E-62 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.282 2.81E-62 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.277 5.12E-60 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.275 2.11E-59 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.275 2.92E-59 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.274 7.72E-59 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.263 6.39E-54 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.257 8.64E-52 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.253 4.99E-50 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.246 1.49E-47 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.240 5.00E-45 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.236 1.04E-43 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.230 1.21E-41 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.229 5.26E-41 YGR174C CBP4 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
0.229 5.47E-41 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p