YBR147W / RTC2

Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR147W / RTC2

Click on Significant Values for Screen Details ID:SGTC_74|Compound:000s-0487|FD-Score:-3.47|P-value:5.31E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.40|P-value:7.19E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.29|P-value:8.52E-7 ID:SGTC_277|Compound:nystatin|FD-Score:-4.04|P-value:3.28E-6 ID:SGTC_286|Compound:k060-0067|FD-Score:2.82|P-value:8.49E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:-2.85|P-value:7.37E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.81|P-value:8.80E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:2.89|P-value:6.49E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:-3.02|P-value:3.71E-4 ID:SGTC_406|Compound:estrone|FD-Score:-3.65|P-value:2.27E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.11|P-value:2.76E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.40|P-value:7.36E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.06|P-value:3.36E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.17|P-value:2.07E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:4.03|P-value:3.61E-6 ID:SGTC_716|Compound:1683-5115|FD-Score:3.71|P-value:1.83E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:2.84|P-value:7.88E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.43|P-value:6.92E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.19|P-value:1.90E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.69|P-value:2.02E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-2.90|P-value:5.97E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-5.12|P-value:5.45E-9 ID:SGTC_1264|Compound:0783-0040|FD-Score:-5.52|P-value:3.49E-10 ID:SGTC_1265|Compound:0819-1001|FD-Score:-5.77|P-value:6.17E-11 ID:SGTC_1300|Compound:1094-0077|FD-Score:2.92|P-value:5.85E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.09|P-value:6.21E-12 ID:SGTC_1385|Compound:3091-4834|FD-Score:2.84|P-value:7.94E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.58|P-value:3.14E-5 ID:SGTC_1742|Compound:st038235|FD-Score:2.91|P-value:6.13E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-2.81|P-value:8.59E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.28|P-value:1.31E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.05|P-value:3.52E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.90|P-value:6.40E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-3.78|P-value:1.19E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-3.11|P-value:2.58E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.21|P-value:1.71E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.26|P-value:1.44E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.92|P-value:6.49E-6 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.70|P-value:1.84E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-2.95|P-value:4.81E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.01|P-value:3.88E-4 ID:SGTC_2534|Compound:helenine|FD-Score:-4.67|P-value:9.36E-8 ID:SGTC_2601|Compound:securinine|FD-Score:-2.85|P-value:7.14E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.14|P-value:2.35E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:4.56|P-value:1.99E-7 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.71|P-value:1.87E-5 ID:SGTC_2808|Compound:7946468|FD-Score:3.26|P-value:1.43E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-3.53|P-value:3.99E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.71|P-value:1.74E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.17|P-value:2.12E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.20|P-value:1.73E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.82|P-value:1.05E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.08|P-value:2.66E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-4.50|P-value:2.59E-7 ID:SGTC_3269|Compound:9137823|FD-Score:-3.33|P-value:9.99E-5 ID:SGTC_3273|Compound:9137859|FD-Score:-3.89|P-value:6.85E-6 ID:SGTC_74|Compound:000s-0487|FD-Score:-3.47|P-value:5.31E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.40|P-value:7.19E-5 ID:SGTC_78|Compound:0336-0123|FD-Score:-4.29|P-value:8.52E-7 ID:SGTC_277|Compound:nystatin|FD-Score:-4.04|P-value:3.28E-6 ID:SGTC_286|Compound:k060-0067|FD-Score:2.82|P-value:8.49E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:-2.85|P-value:7.37E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.81|P-value:8.80E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:2.89|P-value:6.49E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:-3.02|P-value:3.71E-4 ID:SGTC_406|Compound:estrone|FD-Score:-3.65|P-value:2.27E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.11|P-value:2.76E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-3.40|P-value:7.36E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.06|P-value:3.36E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.17|P-value:2.07E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:4.03|P-value:3.61E-6 ID:SGTC_716|Compound:1683-5115|FD-Score:3.71|P-value:1.83E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:2.84|P-value:7.88E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.43|P-value:6.92E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.19|P-value:1.90E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.69|P-value:2.02E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-2.90|P-value:5.97E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-5.12|P-value:5.45E-9 ID:SGTC_1264|Compound:0783-0040|FD-Score:-5.52|P-value:3.49E-10 ID:SGTC_1265|Compound:0819-1001|FD-Score:-5.77|P-value:6.17E-11 ID:SGTC_1300|Compound:1094-0077|FD-Score:2.92|P-value:5.85E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.09|P-value:6.21E-12 ID:SGTC_1385|Compound:3091-4834|FD-Score:2.84|P-value:7.94E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.58|P-value:3.14E-5 ID:SGTC_1742|Compound:st038235|FD-Score:2.91|P-value:6.13E-4 ID:SGTC_1807|Compound:st050092|FD-Score:-2.81|P-value:8.59E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.28|P-value:1.31E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.05|P-value:3.52E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.90|P-value:6.40E-4 ID:SGTC_2019|Compound:4016004|FD-Score:-3.78|P-value:1.19E-5 ID:SGTC_2028|Compound:5153890|FD-Score:-3.11|P-value:2.58E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.21|P-value:1.71E-4 ID:SGTC_2057|Compound:5236571|FD-Score:3.26|P-value:1.44E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.92|P-value:6.49E-6 ID:SGTC_2528|Compound:xanthyletin|FD-Score:-3.70|P-value:1.84E-5 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:-2.95|P-value:4.81E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.01|P-value:3.88E-4 ID:SGTC_2534|Compound:helenine|FD-Score:-4.67|P-value:9.36E-8 ID:SGTC_2601|Compound:securinine|FD-Score:-2.85|P-value:7.14E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:3.14|P-value:2.35E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:4.56|P-value:1.99E-7 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.71|P-value:1.87E-5 ID:SGTC_2808|Compound:7946468|FD-Score:3.26|P-value:1.43E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-3.53|P-value:3.99E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.71|P-value:1.74E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.17|P-value:2.12E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.20|P-value:1.73E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.82|P-value:1.05E-5 ID:SGTC_2978|Compound:9011784|FD-Score:-4.08|P-value:2.66E-6 ID:SGTC_3216|Compound:9129716|FD-Score:-4.50|P-value:2.59E-7 ID:SGTC_3269|Compound:9137823|FD-Score:-3.33|P-value:9.99E-5 ID:SGTC_3273|Compound:9137859|FD-Score:-3.89|P-value:6.85E-6

Top fitness defect scores for YBR147W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1368 1852-0141 6.09 284.0 µM 6.21E-12 ERG2 9 5.82E-10
2 SGTC_2684 capsaicin 4.56 100.0 µM 1.99E-7 12 2.62E-6
3 SGTC_713 1488-1073 4.03 324.0 µM 3.61E-6 ERG2 12 2.76E-5
4 SGTC_2454 5-benzylidenerhodanine 3.92 10.4 µM 6.49E-6 TRP & mitochondrial translation 41 4.45E-5
5 SGTC_2966 9084041 3.82 48.0 µM 1.05E-5 40 6.57E-5
6 SGTC_716 1683-5115 3.71 658.0 µM 1.83E-5 30 1.03E-4
7 SGTC_2749 bexarotene 3.71 57.4 µM 1.87E-5 32 1.05E-4
8 SGTC_1128 3448-9350 3.69 235.0 µM 2.02E-5 27 1.12E-4
9 SGTC_1034 k072-0165 3.43 12.7 µM 6.92E-5 Golgi 39 3.06E-4
10 SGTC_1817 st052116 3.28 64.4 µM 1.31E-4 RNA processing & uracil transport 30 5.17E-4
11 SGTC_2808 7946468 3.26 45.5 µM 1.43E-4 57 5.56E-4
12 SGTC_2057 5236571 3.26 38.3 µM 1.44E-4 RPP1 & pyrimidine depletion 68 5.56E-4
13 SGTC_1043 0916-0009 3.19 19.3 µM 1.90E-4 51 7.00E-4
14 SGTC_538 2922-0838 3.17 53.3 µM 2.07E-4 Golgi 63 7.51E-4
15 SGTC_2850 9015429 3.17 58.4 µM 2.12E-4 42 7.65E-4
16 SGTC_340 k038-0059 3.15 454.0 µM 2.33E-4 ubiquinone biosynthesis & proteasome 71 8.27E-4
17 SGTC_2645 abamectin 3.14 100.0 µM 2.35E-4 heme requiring 60 8.33E-4
18 SGTC_418 lividomycin a 3.11 100.0 µM 2.76E-4 44 9.49E-4
19 SGTC_526 4469-0568 3.06 170.0 µM 3.36E-4 azole & statin 71 0.00112
20 SGTC_811 0416-0114 3.06 265.0 µM 3.38E-4 66 0.00112

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.166 3.93E-22 YJR058C APS2 hom Small subunit of the clathrin-associated adaptor complex AP-2, which is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
0.127 1.28E-13 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.113 4.53E-11 YBR261C TAE1 hom AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
0.113 4.68E-11 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.101 3.94E-9 YDL242W_d YDL242W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.101 3.99E-9 YDR134C YDR134C hom Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication
0.095 3.51E-8 YOR316C-A_p YOR316C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.093 6.07E-8 YKL146W AVT3 hom Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.093 6.21E-8 YCR001W_d YCR001W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene
0.092 8.24E-8 YLR285C-A_p YLR285C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.090 1.95E-7 YKR041W YKR041W hom Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
0.088 2.83E-7 YJL148W RPA34 hom RNA polymerase I subunit A34.5
0.087 5.09E-7 YPR195C_d YPR195C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.077 7.57E-6 YLR108C YLR108C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
0.075 1.37E-5 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed