YBR158W / AMN1

Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR158W / AMN1

Click on Significant Values for Screen Details ID:SGTC_123|Compound:0083-0120|FD-Score:-3.16|P-value:6.05E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.94|P-value:4.51E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.58|P-value:1.25E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:4.48|P-value:4.01E-6 ID:SGTC_213|Compound:1246-0773|FD-Score:3.99|P-value:3.58E-5 ID:SGTC_252|Compound:miconazole|FD-Score:-3.28|P-value:3.93E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-4.35|P-value:4.72E-6 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.18|P-value:8.04E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.16|P-value:8.81E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.23|P-value:6.86E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.16|P-value:6.17E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.30|P-value:5.41E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.43|P-value:3.27E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.69|P-value:1.21E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.20|P-value:1.45E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.33|P-value:4.83E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.02|P-value:2.77E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.13|P-value:9.59E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.44|P-value:3.26E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.77|P-value:8.84E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.69|P-value:8.24E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.31|P-value:5.13E-4 ID:SGTC_1784|Compound:st039334|FD-Score:5.11|P-value:1.71E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.09|P-value:7.81E-4 ID:SGTC_1796|Compound:st049173|FD-Score:-3.60|P-value:1.18E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.14|P-value:9.44E-4 ID:SGTC_2156|Compound:5649887|FD-Score:-3.29|P-value:3.81E-4 ID:SGTC_2270|Compound:7924575|FD-Score:4.62|P-value:2.08E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.77|P-value:9.13E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.43|P-value:3.37E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:3.27|P-value:5.86E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.59|P-value:1.81E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.74|P-value:1.00E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-5.24|P-value:5.01E-8 ID:SGTC_3306|Compound:9127676|FD-Score:3.55|P-value:2.10E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:-3.16|P-value:6.05E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.94|P-value:4.51E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.58|P-value:1.25E-4 ID:SGTC_204|Compound:1543-0184|FD-Score:4.48|P-value:4.01E-6 ID:SGTC_213|Compound:1246-0773|FD-Score:3.99|P-value:3.58E-5 ID:SGTC_252|Compound:miconazole|FD-Score:-3.28|P-value:3.93E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-4.35|P-value:4.72E-6 ID:SGTC_585|Compound:0kpi-0023|FD-Score:3.18|P-value:8.04E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.16|P-value:8.81E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.23|P-value:6.86E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.16|P-value:6.17E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.30|P-value:5.41E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.43|P-value:3.27E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.69|P-value:1.21E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.20|P-value:1.45E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.33|P-value:4.83E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.02|P-value:2.77E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.13|P-value:9.59E-4 ID:SGTC_1645|Compound:Cervolide|FD-Score:3.44|P-value:3.26E-4 ID:SGTC_1649|Compound:st011737|FD-Score:3.77|P-value:8.84E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.69|P-value:8.24E-5 ID:SGTC_1744|Compound:st037937|FD-Score:3.31|P-value:5.13E-4 ID:SGTC_1784|Compound:st039334|FD-Score:5.11|P-value:1.71E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:-3.09|P-value:7.81E-4 ID:SGTC_1796|Compound:st049173|FD-Score:-3.60|P-value:1.18E-4 ID:SGTC_1921|Compound:5740136|FD-Score:3.14|P-value:9.44E-4 ID:SGTC_2156|Compound:5649887|FD-Score:-3.29|P-value:3.81E-4 ID:SGTC_2270|Compound:7924575|FD-Score:4.62|P-value:2.08E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.77|P-value:9.13E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.43|P-value:3.37E-4 ID:SGTC_2648|Compound:atorvastatin|FD-Score:3.27|P-value:5.86E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.59|P-value:1.81E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.74|P-value:1.00E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-5.24|P-value:5.01E-8 ID:SGTC_3306|Compound:9127676|FD-Score:3.55|P-value:2.10E-4

Top fitness defect scores for YBR158W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1784 st039334 5.11 36.3 µM 1.71E-7 6 1.59E-7
2 SGTC_1129 3453-1384 5.02 223.0 µM 2.77E-7 20 2.58E-7
3 SGTC_2270 7924575 4.62 90.9 µM 2.08E-6 RPP1 & pyrimidine depletion 8 1.92E-6
4 SGTC_204 1543-0184 4.48 62.4 µM 4.01E-6 TRP & mitochondrial translation 21 3.69E-6
5 SGTC_1029 k015-0030 4.20 64.7 µM 1.45E-5 21 1.32E-5
6 SGTC_213 1246-0773 3.99 967.0 nM 3.58E-5 19 3.28E-5
7 SGTC_141 0443-0274 3.94 32.3 µM 4.51E-5 mitochondrial response to ROS 40 4.12E-5
8 SGTC_1649 st011737 3.77 51.3 µM 8.84E-5 32 8.06E-5
9 SGTC_2541 muurolladie-3-one 3.77 83.1 µM 9.13E-5 excess fatty acid 38 8.33E-5
10 SGTC_2887 9044784 3.74 58.4 µM 1.00E-4 fatty acid desaturase (OLE1) 34 9.14E-5
11 SGTC_1011 4112-3381 3.69 17.4 µM 1.21E-4 48 1.11E-4
12 SGTC_2661 dactinomycin 3.59 13.0 µM 1.81E-4 redox potentiating 31 1.65E-4
13 SGTC_3306 9127676 3.55 59.8 µM 2.10E-4 34 1.91E-4
14 SGTC_1645 Cervolide 3.44 78.0 µM 3.26E-4 14 2.96E-4
15 SGTC_963 1269-2853 3.43 151.0 µM 3.27E-4 42 2.98E-4
16 SGTC_2561 acetosyringone 3.43 100.0 µM 3.37E-4 47 3.07E-4
17 SGTC_1128 3448-9350 3.33 235.0 µM 4.83E-4 43 4.39E-4
18 SGTC_1744 st037937 3.31 38.9 µM 5.13E-4 51 4.67E-4
19 SGTC_913 3487-1245 3.30 167.0 µM 5.41E-4 63 4.92E-4
20 SGTC_2648 atorvastatin 3.27 78.7 µM 5.86E-4 azole & statin 36 5.33E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 7.03E-18 YGL008C PMA1 het Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.096 2.26E-8 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.094 5.37E-8 YDL134C PPH21 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
0.093 7.16E-8 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.091 1.49E-7 YDL050C_d YDL050C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.086 5.42E-7 YLR099C ICT1 hom Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication
0.080 3.43E-6 YER173W RAD24 hom Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
0.077 8.51E-6 YER139C RTR1 hom CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
0.077 8.97E-6 YDR146C SWI5 hom Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
0.076 1.13E-5 YPR118W MRI1 hom 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
0.075 1.48E-5 YGR181W TIM13 hom Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
0.071 3.63E-5 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.070 4.97E-5 YLR276C DBP9 het DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
0.070 5.46E-5 YHR135C YCK1 hom Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
0.069 7.05E-5 YGL007W_d BRP1_d hom Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1