YBR159W / IFA38

Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR159W / IFA38

Click on Significant Values for Screen Details ID:SGTC_164|Compound:4100-3994|FD-Score:3.40|P-value:3.17E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-4.31|P-value:6.76E-6 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.46|P-value:3.65E-6 ID:SGTC_376|Compound:3970-0862|FD-Score:4.58|P-value:2.15E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.39|P-value:3.28E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.20|P-value:6.25E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.17|P-value:7.37E-4 ID:SGTC_615|Compound:k087-0205|FD-Score:3.63|P-value:1.36E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.37|P-value:3.33E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.39|P-value:3.38E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.50|P-value:2.17E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-3.18|P-value:6.53E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.31|P-value:4.41E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_1612|Compound:st002233|FD-Score:4.04|P-value:2.54E-5 ID:SGTC_1729|Compound:st035615|FD-Score:-5.24|P-value:6.30E-8 ID:SGTC_1733|Compound:st037357|FD-Score:3.66|P-value:1.19E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.16|P-value:7.52E-4 ID:SGTC_2144|Compound:5628481|FD-Score:3.68|P-value:1.11E-4 ID:SGTC_2172|Compound:5754775|FD-Score:-3.53|P-value:1.86E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.11|P-value:9.01E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.15|P-value:7.77E-4 ID:SGTC_2213|Compound:7173844|FD-Score:3.50|P-value:2.21E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.25|P-value:5.19E-4 ID:SGTC_2337|Compound:9009472|FD-Score:5.57|P-value:1.06E-8 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.54|P-value:1.92E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.37|P-value:3.62E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.67|P-value:1.32E-6 ID:SGTC_2481|Compound:5478948|FD-Score:3.74|P-value:8.58E-5 ID:SGTC_2485|Compound:5633444|FD-Score:4.40|P-value:4.91E-6 ID:SGTC_2706|Compound:st078239|FD-Score:3.59|P-value:1.57E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.29|P-value:8.25E-6 ID:SGTC_2933|Compound:9026794|FD-Score:-3.28|P-value:4.61E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.18|P-value:6.59E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.91|P-value:4.25E-5 ID:SGTC_3147|Compound:9097027|FD-Score:4.38|P-value:5.39E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-3.14|P-value:7.48E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.40|P-value:3.17E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-4.31|P-value:6.76E-6 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.46|P-value:3.65E-6 ID:SGTC_376|Compound:3970-0862|FD-Score:4.58|P-value:2.15E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.39|P-value:3.28E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.20|P-value:6.25E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.17|P-value:7.37E-4 ID:SGTC_615|Compound:k087-0205|FD-Score:3.63|P-value:1.36E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.37|P-value:3.33E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.39|P-value:3.38E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.39|P-value:3.30E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.50|P-value:2.17E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-3.18|P-value:6.53E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.31|P-value:4.41E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_1612|Compound:st002233|FD-Score:4.04|P-value:2.54E-5 ID:SGTC_1729|Compound:st035615|FD-Score:-5.24|P-value:6.30E-8 ID:SGTC_1733|Compound:st037357|FD-Score:3.66|P-value:1.19E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.16|P-value:7.52E-4 ID:SGTC_2144|Compound:5628481|FD-Score:3.68|P-value:1.11E-4 ID:SGTC_2172|Compound:5754775|FD-Score:-3.53|P-value:1.86E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.11|P-value:9.01E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.15|P-value:7.77E-4 ID:SGTC_2213|Compound:7173844|FD-Score:3.50|P-value:2.21E-4 ID:SGTC_2331|Compound:7980721|FD-Score:-3.25|P-value:5.19E-4 ID:SGTC_2337|Compound:9009472|FD-Score:5.57|P-value:1.06E-8 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.54|P-value:1.92E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.37|P-value:3.62E-4 ID:SGTC_2471|Compound:5466956|FD-Score:4.67|P-value:1.32E-6 ID:SGTC_2481|Compound:5478948|FD-Score:3.74|P-value:8.58E-5 ID:SGTC_2485|Compound:5633444|FD-Score:4.40|P-value:4.91E-6 ID:SGTC_2706|Compound:st078239|FD-Score:3.59|P-value:1.57E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.29|P-value:8.25E-6 ID:SGTC_2933|Compound:9026794|FD-Score:-3.28|P-value:4.61E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.18|P-value:6.59E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.91|P-value:4.25E-5 ID:SGTC_3147|Compound:9097027|FD-Score:4.38|P-value:5.39E-6 ID:SGTC_3202|Compound:9113761|FD-Score:-3.14|P-value:7.48E-4

Top fitness defect scores for YBR159W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2337 9009472 5.57 200.0 µM 1.06E-8 9 1.24E-8
2 SGTC_2471 5466956 4.67 24.8 µM 1.32E-6 mitochondrial processes 4 1.47E-6
3 SGTC_376 3970-0862 4.58 59.1 µM 2.15E-6 13 2.38E-6
4 SGTC_226 1,3-diallylurea 4.46 8.3 mM 3.65E-6 8 4.01E-6
5 SGTC_2485 5633444 4.40 17.8 µM 4.91E-6 redox potentiating 30 5.38E-6
6 SGTC_3147 9097027 4.38 49.5 µM 5.39E-6 10 5.89E-6
7 SGTC_2760 etidronate 4.29 98.0 µM 8.25E-6 17 8.98E-6
8 SGTC_1612 st002233 4.04 45.4 µM 2.54E-5 15 2.73E-5
9 SGTC_3146 9095609 3.91 49.5 µM 4.25E-5 13 4.55E-5
10 SGTC_2481 5478948 3.74 45.2 µM 8.58E-5 PDR1 42 9.11E-5
11 SGTC_2144 5628481 3.68 200.0 µM 1.11E-4 ERG2 30 1.18E-4
12 SGTC_1733 st037357 3.66 54.9 µM 1.19E-4 60S ribosome export 43 1.26E-4
13 SGTC_615 k087-0205 3.63 167.0 µM 1.36E-4 17 1.44E-4
14 SGTC_2706 st078239 3.59 82.5 µM 1.57E-4 24 1.65E-4
15 SGTC_2420 aureobasidin a 3.54 50.0 nM 1.92E-4 60 2.02E-4
16 SGTC_1315 1237-0019 3.50 3.7 µM 2.17E-4 14 2.29E-4
17 SGTC_2213 7173844 3.50 200.0 µM 2.21E-4 27 2.33E-4
18 SGTC_164 4100-3994 3.40 89.6 µM 3.17E-4 54 3.33E-4
19 SGTC_407 eburnamonine 3.39 100.0 µM 3.28E-4 30 3.44E-4
20 SGTC_1036 k216-0215 3.39 33.5 µM 3.30E-4 mitochondrial response to ROS 77 3.46E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.23E-14 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
0.100 6.76E-9 YGR050C_p YGR050C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.095 3.57E-8 YMR222C FSH2 hom Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
0.090 1.54E-7 YMR113W FOL3 het Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.085 7.80E-7 YBL078C ATG8 hom Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
0.085 9.00E-7 YBR232C_d YBR232C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.084 1.19E-6 YJR052W RAD7 hom Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
0.082 1.85E-6 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.081 2.31E-6 YKL105C SEG2 hom Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
0.080 3.05E-6 YBR285W_p YBR285W_p hom Putative protein of unknown function; YBR285W is not an essential gene
0.078 6.20E-6 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.077 7.11E-6 YBR072W HSP26 hom Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
0.075 1.53E-5 YLL049W LDB18 hom Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
0.074 1.60E-5 YKR077W MSA2 hom Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
0.074 1.61E-5 YGR268C HUA1 hom Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly