YBR170C / NPL4

Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR170C / NPL4

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-4.32|P-value:9.10E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.94|P-value:4.62E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:-8.18|P-value:2.37E-16 ID:SGTC_142|Compound:0335-0849|FD-Score:-4.12|P-value:2.18E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:4.75|P-value:1.27E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.27|P-value:5.90E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.57|P-value:1.68E-8 ID:SGTC_264|Compound:promethazine|FD-Score:7.62|P-value:2.15E-14 ID:SGTC_310|Compound:1273-0058|FD-Score:3.27|P-value:6.01E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.35|P-value:7.97E-6 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.48|P-value:2.79E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.41|P-value:3.65E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:5.28|P-value:8.22E-8 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.78|P-value:8.82E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:3.60|P-value:1.76E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:4.01|P-value:3.47E-5 ID:SGTC_987|Compound:1497-1013|FD-Score:-4.48|P-value:4.34E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.62|P-value:1.66E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.21|P-value:1.47E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.39|P-value:3.77E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.57|P-value:2.03E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:-5.92|P-value:2.13E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.49|P-value:2.62E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.55|P-value:2.18E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.41|P-value:3.58E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-3.72|P-value:1.09E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.91|P-value:5.26E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.21|P-value:7.43E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.83|P-value:7.20E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.93|P-value:4.94E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-4.85|P-value:7.50E-7 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.21|P-value:7.41E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.79|P-value:8.28E-5 ID:SGTC_2698|Compound:st077697|FD-Score:-3.65|P-value:1.44E-4 ID:SGTC_2713|Compound:st079149|FD-Score:4.18|P-value:1.72E-5 ID:SGTC_2746|Compound:daunorubicin|FD-Score:-3.34|P-value:4.58E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:4.12|P-value:2.25E-5 ID:SGTC_2803|Compound:7707263|FD-Score:-3.12|P-value:9.82E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.69|P-value:1.25E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.99|P-value:3.69E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.41|P-value:3.60E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.52|P-value:2.43E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.12|P-value:9.97E-4 ID:SGTC_3165|Compound:9101544|FD-Score:-4.27|P-value:1.13E-5 ID:SGTC_3175|Compound:9106517|FD-Score:3.55|P-value:2.15E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.78|P-value:1.05E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.17|P-value:8.29E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.32|P-value:9.10E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.94|P-value:4.62E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:-8.18|P-value:2.37E-16 ID:SGTC_142|Compound:0335-0849|FD-Score:-4.12|P-value:2.18E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:4.75|P-value:1.27E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.27|P-value:5.90E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:5.57|P-value:1.68E-8 ID:SGTC_264|Compound:promethazine|FD-Score:7.62|P-value:2.15E-14 ID:SGTC_310|Compound:1273-0058|FD-Score:3.27|P-value:6.01E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.35|P-value:7.97E-6 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.48|P-value:2.79E-4 ID:SGTC_556|Compound:4058-0063|FD-Score:3.41|P-value:3.65E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:5.28|P-value:8.22E-8 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.78|P-value:8.82E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:3.60|P-value:1.76E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:4.01|P-value:3.47E-5 ID:SGTC_987|Compound:1497-1013|FD-Score:-4.48|P-value:4.34E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:3.62|P-value:1.66E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.21|P-value:1.47E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.39|P-value:3.77E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.57|P-value:2.03E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:-5.92|P-value:2.13E-9 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.49|P-value:2.62E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.55|P-value:2.18E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.41|P-value:3.58E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-3.72|P-value:1.09E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.91|P-value:5.26E-5 ID:SGTC_2313|Compound:7726885|FD-Score:3.21|P-value:7.43E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.83|P-value:7.20E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.93|P-value:4.94E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-4.85|P-value:7.50E-7 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.21|P-value:7.41E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.79|P-value:8.28E-5 ID:SGTC_2698|Compound:st077697|FD-Score:-3.65|P-value:1.44E-4 ID:SGTC_2713|Compound:st079149|FD-Score:4.18|P-value:1.72E-5 ID:SGTC_2746|Compound:daunorubicin|FD-Score:-3.34|P-value:4.58E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:4.12|P-value:2.25E-5 ID:SGTC_2803|Compound:7707263|FD-Score:-3.12|P-value:9.82E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.69|P-value:1.25E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.99|P-value:3.69E-5 ID:SGTC_2878|Compound:9043973|FD-Score:-3.41|P-value:3.60E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.52|P-value:2.43E-4 ID:SGTC_3094|Compound:9117742|FD-Score:3.12|P-value:9.97E-4 ID:SGTC_3165|Compound:9101544|FD-Score:-4.27|P-value:1.13E-5 ID:SGTC_3175|Compound:9106517|FD-Score:3.55|P-value:2.15E-4 ID:SGTC_3218|Compound:9131285|FD-Score:4.78|P-value:1.05E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.17|P-value:8.29E-4

Top fitness defect scores for YBR170C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 7.62 12.5 mM 2.15E-14 DNA intercalators 14 1.32E-14
2 SGTC_257 daunorubicin 5.57 18.5 µM 1.68E-8 anthracycline transcription coupled DNA repair 12 1.29E-8
3 SGTC_568 1193-0097 5.28 7.5 µM 8.22E-8 ERAD & cell cycle 78 6.44E-8
4 SGTC_3218 9131285 4.78 49.5 µM 1.05E-6 endomembrane recycling 20 8.58E-7
5 SGTC_145 3737-0113 4.75 38.4 µM 1.27E-6 DNA intercalators 52 1.04E-6
6 SGTC_312 4092-0653 4.35 6.8 µM 7.97E-6 ergosterol depletion effects on membrane 74 6.73E-6
7 SGTC_1082 camptothecin 4.21 424.9 nM 1.47E-5 DNA damage response 40 1.25E-5
8 SGTC_2713 st079149 4.18 53.4 µM 1.72E-5 13 1.47E-5
9 SGTC_2777 climbazole 4.12 1.5 µM 2.25E-5 iron homeostasis 41 1.93E-5
10 SGTC_926 2268-1401 4.01 59.4 µM 3.47E-5 TSC3-RPN4 26 3.00E-5
11 SGTC_77 0828-0286 3.94 12.5 µM 4.62E-5 TSC3-RPN4 37 4.01E-5
12 SGTC_2489 cantharidin 3.93 73.4 µM 4.94E-5 cell wall signaling 42 4.29E-5
13 SGTC_1951 sophoraflavanone G 3.91 9.2 µM 5.26E-5 RNA processing & uracil transport 23 4.58E-5
14 SGTC_2315 7733312 3.83 200.0 µM 7.20E-5 31 6.30E-5
15 SGTC_1065 prochlorperazine maleate 3.62 2.5 µM 1.66E-4 20 1.48E-4
16 SGTC_867 0518-0118 3.60 155.0 µM 1.76E-4 DNA intercalators 29 1.57E-4
17 SGTC_1137 ixabepilone 3.57 100.0 µM 2.03E-4 42 1.80E-4
18 SGTC_3175 9106517 3.55 49.5 µM 2.15E-4 iron homeostasis 23 1.91E-4
19 SGTC_1299 1079-1439 3.55 3.7 µM 2.18E-4 TSC3-RPN4 20 1.94E-4
20 SGTC_2965 9082772 3.52 38.6 µM 2.43E-4 mitochondrial response to ROS 52 2.17E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.263 3.65E-54 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.259 1.77E-52 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.255 5.99E-51 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.243 4.99E-46 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.219 8.30E-38 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.218 1.44E-37 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.215 2.19E-36 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.207 8.00E-34 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.197 1.17E-30 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.183 1.05E-26 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.175 1.72E-24 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.172 1.02E-23 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.171 1.76E-23 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.148 7.76E-18 YLR133W CKI1 hom Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
0.142 1.55E-16 YOR064C YNG1 hom Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1