YBR216C / YBP1

Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR216C / YBP1

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:3.14|P-value:6.11E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:5.04|P-value:1.08E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:6.04|P-value:2.67E-10 ID:SGTC_212|Compound:3807-4682|FD-Score:6.05|P-value:2.51E-10 ID:SGTC_213|Compound:1246-0773|FD-Score:3.95|P-value:2.36E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.59|P-value:1.07E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.01|P-value:9.52E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.45|P-value:1.89E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.10|P-value:6.88E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.60|P-value:1.13E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.64|P-value:8.94E-5 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.04|P-value:9.44E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.00|P-value:9.87E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.21|P-value:4.70E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.20|P-value:4.89E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:4.92|P-value:2.05E-7 ID:SGTC_990|Compound:3985-0011|FD-Score:3.31|P-value:3.26E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.47|P-value:2.07E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:4.00|P-value:1.94E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.34|P-value:2.95E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.52|P-value:1.56E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.67|P-value:7.67E-5 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.54|P-value:1.30E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.25|P-value:4.44E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.37|P-value:3.90E-6 ID:SGTC_1889|Compound:st059080|FD-Score:3.25|P-value:4.08E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.74|P-value:5.76E-5 ID:SGTC_1901|Compound:st060216|FD-Score:-5.05|P-value:1.14E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.76|P-value:5.62E-7 ID:SGTC_2077|Compound:5212015|FD-Score:3.11|P-value:6.77E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.31|P-value:3.24E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.24|P-value:4.18E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.38|P-value:2.45E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.00|P-value:9.81E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.34|P-value:2.89E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.36|P-value:2.65E-4 ID:SGTC_3003|Compound:9075612|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.17|P-value:5.50E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.22|P-value:4.61E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.48|P-value:1.89E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.27|P-value:3.74E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.02|P-value:9.16E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.14|P-value:6.11E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:5.04|P-value:1.08E-7 ID:SGTC_210|Compound:3551-0445|FD-Score:6.04|P-value:2.67E-10 ID:SGTC_212|Compound:3807-4682|FD-Score:6.05|P-value:2.51E-10 ID:SGTC_213|Compound:1246-0773|FD-Score:3.95|P-value:2.36E-5 ID:SGTC_216|Compound:k038-0035|FD-Score:3.59|P-value:1.07E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.01|P-value:9.52E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.45|P-value:1.89E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.10|P-value:6.88E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.60|P-value:1.13E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:3.64|P-value:8.94E-5 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.04|P-value:9.44E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.00|P-value:9.87E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:3.21|P-value:4.70E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.20|P-value:4.89E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:4.92|P-value:2.05E-7 ID:SGTC_990|Compound:3985-0011|FD-Score:3.31|P-value:3.26E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.47|P-value:2.07E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:4.00|P-value:1.94E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.34|P-value:2.95E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.52|P-value:1.56E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.67|P-value:7.67E-5 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:3.54|P-value:1.30E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.25|P-value:4.44E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.37|P-value:3.90E-6 ID:SGTC_1889|Compound:st059080|FD-Score:3.25|P-value:4.08E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.74|P-value:5.76E-5 ID:SGTC_1901|Compound:st060216|FD-Score:-5.05|P-value:1.14E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.76|P-value:5.62E-7 ID:SGTC_2077|Compound:5212015|FD-Score:3.11|P-value:6.77E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.31|P-value:3.24E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.24|P-value:4.18E-4 ID:SGTC_2428|Compound:5615643|FD-Score:3.38|P-value:2.45E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.00|P-value:9.81E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.34|P-value:2.89E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.36|P-value:2.65E-4 ID:SGTC_3003|Compound:9075612|FD-Score:3.53|P-value:1.37E-4 ID:SGTC_3084|Compound:9116877|FD-Score:3.17|P-value:5.50E-4 ID:SGTC_3096|Compound:9117468|FD-Score:3.22|P-value:4.61E-4 ID:SGTC_3191|Compound:9110352|FD-Score:-3.48|P-value:1.89E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.27|P-value:3.74E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.02|P-value:9.16E-4

Top fitness defect scores for YBR216C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_212 3807-4682 6.05 38.8 µM 2.51E-10 tubulin folding & SWR complex 26 7.46E-10
2 SGTC_210 3551-0445 6.04 56.8 µM 2.67E-10 26 7.91E-10
3 SGTC_149 3970-0822 5.04 46.1 µM 1.08E-7 tubulin folding & SWR complex 47 2.35E-7
4 SGTC_910 3454-3003 4.92 490.0 µM 2.05E-7 5 4.31E-7
5 SGTC_1002 4049-0206 4.47 172.0 µM 2.07E-6 18 3.86E-6
6 SGTC_1004 4084-0029 4.00 199.0 µM 1.94E-5 15 3.23E-5
7 SGTC_213 1246-0773 3.95 967.0 nM 2.36E-5 20 3.89E-5
8 SGTC_1892 st058411 3.74 7.4 µM 5.76E-5 28 9.06E-5
9 SGTC_1325 1431-0015 3.67 227.0 µM 7.67E-5 Golgi 25 1.19E-4
10 SGTC_646 4092-0839 3.64 27.3 µM 8.94E-5 plasma membrane duress 33 1.38E-4
11 SGTC_216 k038-0035 3.59 28.4 µM 1.07E-4 ubiquinone biosynthesis & proteasome 58 1.63E-4
12 SGTC_1552 6-methylflavone 3.54 10.6 µM 1.30E-4 superoxide 24 1.96E-4
13 SGTC_3003 9075612 3.53 71.4 µM 1.37E-4 29 2.07E-4
14 SGTC_283 0987-0079 3.45 11.7 µM 1.89E-4 44 2.80E-4
15 SGTC_2428 5615643 3.38 7.8 µM 2.45E-4 45 3.58E-4
16 SGTC_2752 diclazuril 3.36 33.1 µM 2.65E-4 165 3.86E-4
17 SGTC_2588 indole-3-carbinol 3.34 100.0 µM 2.89E-4 74 4.19E-4
18 SGTC_1083 didemethylchlorpromazine sulfoxide 3.34 707.4 nM 2.95E-4 redox potentiating 122 4.26E-4
19 SGTC_2130 NSC 7 3.31 2.5 µM 3.24E-4 copper-dependent oxidative stress 62 4.67E-4
20 SGTC_990 3985-0011 3.31 41.3 µM 3.26E-4 amide catabolism 38 4.70E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.228 1.11E-40 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.221 3.03E-38 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.161 7.87E-21 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.160 1.47E-20 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.156 1.24E-19 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.152 8.10E-19 YML007W YAP1 hom Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.132 1.53E-14 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 1.61E-14 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.126 2.03E-13 YDR334W SWR1 hom Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.126 2.51E-13 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.126 2.56E-13 YMR138W CIN4 hom GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
0.125 3.54E-13 YDR242W AMD2 hom Putative amidase
0.124 6.38E-13 YDL146W LDB17 hom Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
0.121 2.00E-12 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.121 2.09E-12 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C