YBR217W / ATG12

Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR217W / ATG12

Click on Significant Values for Screen Details ID:SGTC_597|Compound:k018-0003|FD-Score:-3.57|P-value:5.25E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.57|P-value:3.99E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.56|P-value:4.14E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-3.65|P-value:3.99E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.85|P-value:1.54E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.76|P-value:2.75E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.30|P-value:3.73E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.77|P-value:2.64E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.78|P-value:1.95E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.62|P-value:3.43E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.20|P-value:5.85E-7 ID:SGTC_1816|Compound:st051957|FD-Score:3.59|P-value:3.84E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.50|P-value:6.40E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-6.82|P-value:1.46E-10 ID:SGTC_1960|Compound:st011366|FD-Score:3.56|P-value:4.18E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-5.60|P-value:1.17E-7 ID:SGTC_2013|Compound:4024628|FD-Score:-3.53|P-value:5.90E-4 ID:SGTC_2108|Compound:5365811|FD-Score:4.52|P-value:1.16E-5 ID:SGTC_2114|Compound:5491910|FD-Score:-3.88|P-value:1.77E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.39|P-value:9.15E-4 ID:SGTC_2298|Compound:7976326|FD-Score:4.05|P-value:7.49E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.97|P-value:9.80E-5 ID:SGTC_2324|Compound:6149169|FD-Score:4.67|P-value:6.12E-6 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.86|P-value:2.69E-6 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.08|P-value:6.59E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:4.57|P-value:9.25E-6 ID:SGTC_2714|Compound:st079313|FD-Score:3.98|P-value:9.70E-5 ID:SGTC_2837|Compound:9008476|FD-Score:3.54|P-value:4.52E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.84|P-value:1.59E-4 ID:SGTC_2988|Compound:9073640|FD-Score:3.42|P-value:6.59E-4 ID:SGTC_2993|Compound:9054257|FD-Score:5.81|P-value:2.85E-8 ID:SGTC_3025|Compound:9087895|FD-Score:-3.94|P-value:1.44E-4 ID:SGTC_3042|Compound:9091578|FD-Score:-5.32|P-value:4.63E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.34|P-value:8.70E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.65|P-value:3.91E-4 ID:SGTC_3127|Compound:9126946|FD-Score:4.78|P-value:3.82E-6 ID:SGTC_3137|Compound:9097352|FD-Score:5.64|P-value:6.74E-8 ID:SGTC_3141|Compound:9094609|FD-Score:-5.72|P-value:6.40E-8 ID:SGTC_3193|Compound:9111931|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.24|P-value:3.53E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.57|P-value:5.25E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.57|P-value:3.99E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:3.56|P-value:4.14E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-3.65|P-value:3.99E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.85|P-value:1.54E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.76|P-value:2.75E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.30|P-value:3.73E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.77|P-value:2.64E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.78|P-value:1.95E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.62|P-value:3.43E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.20|P-value:5.85E-7 ID:SGTC_1816|Compound:st051957|FD-Score:3.59|P-value:3.84E-4 ID:SGTC_1912|Compound:st060209|FD-Score:-3.50|P-value:6.40E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-6.82|P-value:1.46E-10 ID:SGTC_1960|Compound:st011366|FD-Score:3.56|P-value:4.18E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-5.60|P-value:1.17E-7 ID:SGTC_2013|Compound:4024628|FD-Score:-3.53|P-value:5.90E-4 ID:SGTC_2108|Compound:5365811|FD-Score:4.52|P-value:1.16E-5 ID:SGTC_2114|Compound:5491910|FD-Score:-3.88|P-value:1.77E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.39|P-value:9.15E-4 ID:SGTC_2298|Compound:7976326|FD-Score:4.05|P-value:7.49E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.97|P-value:9.80E-5 ID:SGTC_2324|Compound:6149169|FD-Score:4.67|P-value:6.12E-6 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:4.86|P-value:2.69E-6 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.08|P-value:6.59E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:4.57|P-value:9.25E-6 ID:SGTC_2714|Compound:st079313|FD-Score:3.98|P-value:9.70E-5 ID:SGTC_2837|Compound:9008476|FD-Score:3.54|P-value:4.52E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.84|P-value:1.59E-4 ID:SGTC_2988|Compound:9073640|FD-Score:3.42|P-value:6.59E-4 ID:SGTC_2993|Compound:9054257|FD-Score:5.81|P-value:2.85E-8 ID:SGTC_3025|Compound:9087895|FD-Score:-3.94|P-value:1.44E-4 ID:SGTC_3042|Compound:9091578|FD-Score:-5.32|P-value:4.63E-7 ID:SGTC_3072|Compound:9116419|FD-Score:3.34|P-value:8.70E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.65|P-value:3.91E-4 ID:SGTC_3127|Compound:9126946|FD-Score:4.78|P-value:3.82E-6 ID:SGTC_3137|Compound:9097352|FD-Score:5.64|P-value:6.74E-8 ID:SGTC_3141|Compound:9094609|FD-Score:-5.72|P-value:6.40E-8 ID:SGTC_3193|Compound:9111931|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.24|P-value:3.53E-5

Top fitness defect scores for YBR217W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2993 9054257 5.81 71.4 µM 2.85E-8 fatty acid desaturase (OLE1) 18 3.15E-9
2 SGTC_3137 9097352 5.64 49.5 µM 6.74E-8 9 8.45E-9
3 SGTC_1635 st007035 5.20 42.4 µM 5.85E-7 161 1.01E-7
4 SGTC_2538 3',7'-epoxycaryophyllan-6-one 4.86 100.0 µM 2.69E-6 11 5.78E-7
5 SGTC_3127 9126946 4.78 49.5 µM 3.82E-6 ERG2 9 8.65E-7
6 SGTC_2324 6149169 4.67 200.0 µM 6.12E-6 13 1.49E-6
7 SGTC_2607 solasodine 4.57 47.4 µM 9.25E-6 41 2.38E-6
8 SGTC_2108 5365811 4.52 200.0 µM 1.16E-5 7 3.08E-6
9 SGTC_3198 9112019 4.24 49.5 µM 3.53E-5 mitochondrial processes 10 1.10E-5
10 SGTC_2539 epi(13)torulosol 4.08 80.0 µM 6.59E-5 22 2.25E-5
11 SGTC_2298 7976326 4.05 193.8 µM 7.49E-5 7 2.60E-5
12 SGTC_2714 st079313 3.98 56.9 µM 9.70E-5 9 3.49E-5
13 SGTC_2321 7390090 3.97 138.8 µM 9.80E-5 26 3.53E-5
14 SGTC_1255 0566-0045 3.85 221.0 µM 1.54E-4 excess fatty acid 33 5.94E-5
15 SGTC_2933 9026794 3.84 32.5 µM 1.59E-4 22 6.13E-5
16 SGTC_1528 arabinosylhypoxanthine 3.78 74.6 µM 1.95E-4 28 7.76E-5
17 SGTC_1630 st005209 3.62 31.3 µM 3.43E-4 32 1.48E-4
18 SGTC_1816 st051957 3.59 91.6 µM 3.84E-4 17 1.68E-4
19 SGTC_785 1270-0111 3.57 65.3 µM 3.99E-4 43 1.75E-4
20 SGTC_948 3448-0295 3.56 75.3 µM 4.14E-4 41 1.83E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 5.26E-18 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.146 1.90E-17 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.138 1.23E-15 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.134 7.66E-15 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.130 4.32E-14 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.128 9.09E-14 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.124 7.00E-13 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.123 1.09E-12 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.121 2.03E-12 YLR280C_d YLR280C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.119 4.09E-12 YDR197W CBS2 hom Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
0.117 1.16E-11 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.117 1.31E-11 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.116 1.81E-11 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.116 1.82E-11 YOR301W RAX1 hom Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily
0.115 2.64E-11 YKL128C PMU1 hom Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant