YBR228W / SLX1

Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR228W / SLX1

Click on Significant Values for Screen Details ID:SGTC_160|Compound:3453-0671|FD-Score:3.97|P-value:1.10E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.38|P-value:8.05E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.83|P-value:1.81E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.80|P-value:2.02E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.90|P-value:1.93E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.18|P-value:5.07E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.88|P-value:1.52E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.21|P-value:4.55E-5 ID:SGTC_1906|Compound:st060206|FD-Score:-4.03|P-value:1.22E-4 ID:SGTC_1932|Compound:st074806|FD-Score:4.03|P-value:8.80E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-5.45|P-value:3.22E-7 ID:SGTC_1950|Compound:st077106|FD-Score:4.45|P-value:1.74E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:6.18|P-value:4.91E-9 ID:SGTC_2089|Compound:5281248|FD-Score:3.37|P-value:8.36E-4 ID:SGTC_2125|Compound:5246583|FD-Score:5.51|P-value:1.54E-7 ID:SGTC_2264|Compound:7948757|FD-Score:-3.77|P-value:2.95E-4 ID:SGTC_2267|Compound:7931562|FD-Score:5.27|P-value:4.88E-7 ID:SGTC_2279|Compound:7948423|FD-Score:-5.34|P-value:5.45E-7 ID:SGTC_2293|Compound:7624732|FD-Score:3.43|P-value:6.99E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.81|P-value:2.66E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.71|P-value:6.02E-6 ID:SGTC_2477|Compound:5967686|FD-Score:3.65|P-value:3.29E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.93|P-value:1.27E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:4.05|P-value:8.14E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.51|P-value:1.36E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.46|P-value:1.97E-7 ID:SGTC_2794|Compound:7785382|FD-Score:-3.74|P-value:3.33E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.59|P-value:4.06E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.67|P-value:3.08E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.71|P-value:3.64E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-5.54|P-value:2.12E-7 ID:SGTC_2975|Compound:9084847|FD-Score:-5.45|P-value:3.13E-7 ID:SGTC_2990|Compound:9074105|FD-Score:3.32|P-value:9.80E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-4.48|P-value:2.24E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-4.24|P-value:5.46E-5 ID:SGTC_3348|Compound:9149464|FD-Score:4.43|P-value:1.91E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.35|P-value:8.95E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:3.97|P-value:1.10E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.38|P-value:8.05E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.83|P-value:1.81E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.80|P-value:2.02E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.90|P-value:1.93E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.18|P-value:5.07E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.88|P-value:1.52E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.21|P-value:4.55E-5 ID:SGTC_1906|Compound:st060206|FD-Score:-4.03|P-value:1.22E-4 ID:SGTC_1932|Compound:st074806|FD-Score:4.03|P-value:8.80E-5 ID:SGTC_1945|Compound:st075991|FD-Score:-5.45|P-value:3.22E-7 ID:SGTC_1950|Compound:st077106|FD-Score:4.45|P-value:1.74E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:6.18|P-value:4.91E-9 ID:SGTC_2089|Compound:5281248|FD-Score:3.37|P-value:8.36E-4 ID:SGTC_2125|Compound:5246583|FD-Score:5.51|P-value:1.54E-7 ID:SGTC_2264|Compound:7948757|FD-Score:-3.77|P-value:2.95E-4 ID:SGTC_2267|Compound:7931562|FD-Score:5.27|P-value:4.88E-7 ID:SGTC_2279|Compound:7948423|FD-Score:-5.34|P-value:5.45E-7 ID:SGTC_2293|Compound:7624732|FD-Score:3.43|P-value:6.99E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-3.81|P-value:2.66E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.71|P-value:6.02E-6 ID:SGTC_2477|Compound:5967686|FD-Score:3.65|P-value:3.29E-4 ID:SGTC_2485|Compound:5633444|FD-Score:3.93|P-value:1.27E-4 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:4.05|P-value:8.14E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.51|P-value:1.36E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.46|P-value:1.97E-7 ID:SGTC_2794|Compound:7785382|FD-Score:-3.74|P-value:3.33E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.59|P-value:4.06E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.67|P-value:3.08E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.71|P-value:3.64E-4 ID:SGTC_2967|Compound:9089538|FD-Score:-5.54|P-value:2.12E-7 ID:SGTC_2975|Compound:9084847|FD-Score:-5.45|P-value:3.13E-7 ID:SGTC_2990|Compound:9074105|FD-Score:3.32|P-value:9.80E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-4.48|P-value:2.24E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-4.24|P-value:5.46E-5 ID:SGTC_3348|Compound:9149464|FD-Score:4.43|P-value:1.91E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.35|P-value:8.95E-4

Top fitness defect scores for YBR228W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1955 wogonin 6.18 32.4 µM 4.91E-9 4 3.17E-10
2 SGTC_2125 5246583 5.51 200.0 µM 1.54E-7 tubulin folding & SWR complex 19 1.76E-8
3 SGTC_2581 avocatin a 5.46 64.0 µM 1.97E-7 sphingolipid biosynthesis & PDR1 26 2.34E-8
4 SGTC_2267 7931562 5.27 200.0 µM 4.88E-7 2 6.73E-8
5 SGTC_2414 st077232 4.71 92.9 µM 6.02E-6 12 1.25E-6
6 SGTC_2563 tomatine 4.51 8.5 µM 1.36E-5 27 3.22E-6
7 SGTC_1950 st077106 4.45 7.2 µM 1.74E-5 RNA processing & uracil transport 17 4.28E-6
8 SGTC_3348 9149464 4.43 3.5 µM 1.91E-5 8 4.77E-6
9 SGTC_1354 1498-1681 4.21 81.0 µM 4.55E-5 13 1.30E-5
10 SGTC_1342 1486-1464 4.18 234.0 µM 5.07E-5 11 1.48E-5
11 SGTC_2529 gangaleoidin 4.05 93.6 µM 8.14E-5 15 2.56E-5
12 SGTC_1932 st074806 4.03 40.9 µM 8.80E-5 25 2.80E-5
13 SGTC_160 3453-0671 3.97 289.7 µM 1.10E-4 39 3.64E-5
14 SGTC_2485 5633444 3.93 17.8 µM 1.27E-4 redox potentiating 45 4.28E-5
15 SGTC_1348 1493-0373 3.88 93.8 µM 1.52E-4 15 5.25E-5
16 SGTC_711 k292-0785 3.83 179.0 µM 1.81E-4 DNA intercalators 27 6.45E-5
17 SGTC_1285 0929-0069 3.80 65.5 µM 2.02E-4 19 7.30E-5
18 SGTC_2830 7992007 3.67 71.4 µM 3.08E-4 28 1.19E-4
19 SGTC_2477 5967686 3.65 25.2 µM 3.29E-4 34 1.29E-4
20 SGTC_2800 7887935 3.59 81.8 µM 4.06E-4 RPP1 & pyrimidine depletion 44 1.64E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.117 1.24E-11 YGL164C YRB30 hom RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
0.108 4.02E-10 YDL028C MPS1 het Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
0.104 1.69E-9 YNL162W-A_p YNL162W-A_p hom Putative protein of unknown function; identified by homology
0.098 1.28E-8 YIR015W RPR2 het Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
0.091 1.50E-7 YKL150W MCR1 hom Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis
0.089 2.41E-7 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.089 2.50E-7 YGR017W_p YGR017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
0.083 1.67E-6 YDR311W TFB1 het Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
0.080 3.73E-6 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.073 2.08E-5 YER036C ARB1 het ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
0.073 2.59E-5 YCL049C_p YCL049C_p hom Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene
0.072 2.70E-5 YBR249C ARO4 hom 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
0.071 3.72E-5 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.071 3.76E-5 YKL075C_p YKL075C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
0.071 4.05E-5 YFR028C CDC14 het Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis