HIPHOP chemogenomics database

YBR256C / RIB5

Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway

Zygosity: Heterozygous strain

fixedexpanded
Profile for YBR256C / RIB5

Click on Significant Values for Screen Details

Top fitness defect scores for YBR256C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2428	5615643	6.30	7.8 µM	9.23E-11		2	1.51E-10	0.23
2	SGTC_3036	9090181	5.02	49.5 µM	1.83E-7		2	2.61E-7	0.83
3	SGTC_2599	2',3-dihydroxy-4,4',6'-trimethoxychalcone	4.52	100.0 µM	2.31E-6		4	3.16E-6	0.01
4	SGTC_426	0988-0037	4.29	14.6 µM	6.83E-6	ERAD & cell cycle	10	9.13E-6	0.17
5	SGTC_323	4013-0906	4.25	34.3 µM	8.03E-6	ERG2	2	1.07E-5	0.60
6	SGTC_1853	st056710	4.25	91.2 µM	8.14E-6	amide catabolism	5	1.09E-5	0.32
7	SGTC_1716	st033144	4.12	30.6 µM	1.41E-5		6	1.86E-5	0.31
8	SGTC_168	3970-0862	4.03	530.5 µM	2.12E-5		43	2.78E-5	0.03
9	SGTC_1715	st032160	3.99	10.2 µM	2.55E-5		5	3.32E-5	0.17
10	SGTC_1464	k208-0027	3.91	110.0 µM	3.50E-5		6	4.54E-5	0.47
11	SGTC_2620	irigenin	3.84	100.0 µM	4.68E-5		16	6.03E-5	0.15
12	SGTC_2350	9023895	3.84	38.9 µM	4.78E-5	ergosterol biosynthesis	9	6.16E-5	0.11
13	SGTC_2568	diosmetin	3.76	100.0 µM	6.71E-5		13	8.59E-5	0.04
14	SGTC_25	0118-0223	3.69	35.7 µM	8.94E-5		3	1.14E-4	0.39
15	SGTC_3239	9133757	3.66	49.5 µM	9.74E-5		1	1.24E-4	0.52
16	SGTC_1993	st073995	3.47	70.8 µM	2.05E-4		9	2.56E-4	0.04
17	SGTC_2076	5212012	3.44	200.0 µM	2.32E-4		3	2.89E-4	0.37
18	SGTC_2569	tetrandrine	3.44	100.0 µM	2.35E-4		8	2.93E-4	0.02
19	SGTC_2596	sinensetin	3.40	100.0 µM	2.73E-4		12	3.40E-4	0.09
20	SGTC_2723	salbutamol	3.26	83.6 µM	4.58E-4		2	5.64E-4	0.37

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.182	1.84E-26	YOR159C	SME1	het	Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.127	1.69E-13	YER136W	GDI1	het	GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.123	8.18E-13	YCR048W	ARE1	hom	Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
0.123	8.76E-13	YER013W	PRP22	het	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.119	4.30E-12	YDL060W	TSR1	het	Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.115	2.16E-11	YBR118W	TEF2	hom	Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.113	5.55E-11	YGR086C	PIL1	hom	Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.110	1.50E-10	YKL164C	PIR1	hom	O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.110	1.99E-10	YGR207C	CIR1	hom	Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.106	7.22E-10	YLR127C	APC2	het	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
0.103	1.87E-9	YDR044W	HEM13	het	Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.103	2.15E-9	YDL209C	CWC2	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.103	2.56E-9	YNL132W	KRE33	het	Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.101	4.29E-9	YGL208W	SIP2	hom	One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane
0.100	6.68E-9	YDR277C	MTH1	hom	Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation