Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
Profile for YBR259W / YBR259W

Click on Significant Values for Screen Details

Top fitness defect scores for YBR259W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2588 indole-3-carbinol 9.04 100.0 µM 1.43E-19 2 8.14E-20
2 SGTC_943 3253-1379 6.98 105.0 µM 2.04E-12 2 1.45E-12
3 SGTC_2407 paclitaxel 5.45 400.0 µM 3.11E-8 11 2.52E-8
4 SGTC_2393 9031399 4.85 185.9 µM 7.44E-7 RNA processing & uracil transport 14 6.27E-7
5 SGTC_2966 9084041 4.58 48.0 µM 2.74E-6 20 2.36E-6
6 SGTC_2940 9060638 4.40 45.9 µM 6.19E-6 amide catabolism 14 5.37E-6
7 SGTC_70 0141-0289 4.18 43.3 µM 1.67E-5 mitochondrial stress 155 1.47E-5
8 SGTC_2981 9013952 4.16 39.0 µM 1.81E-5 36 1.60E-5
9 SGTC_178 4092-0806 4.07 87.5 µM 2.61E-5 ergosterol depletion effects on membrane 90 2.30E-5
10 SGTC_2802 7925815 4.02 54.5 µM 3.23E-5 39 2.86E-5
11 SGTC_1979 st071809 4.00 45.3 µM 3.64E-5 RPP1 & pyrimidine depletion 13 3.23E-5
12 SGTC_134 0269-0018 3.99 6.9 µM 3.75E-5 134 3.33E-5
13 SGTC_1273 0849-0096 3.80 14.1 µM 8.12E-5 RPP1 & pyrimidine depletion 22 7.29E-5
14 SGTC_2502 avocadyne acetate 3.76 18.6 µM 9.46E-5 28 8.51E-5
15 SGTC_122 0236-0016 3.72 402.4 µM 1.09E-4 RNA processing & uracil transport 39 9.82E-5
16 SGTC_2794 7785382 3.72 27.3 µM 1.10E-4 46 9.92E-5
17 SGTC_1861 st057647 3.60 17.4 µM 1.73E-4 30 1.57E-4
18 SGTC_131 0141-0277 3.54 33.9 µM 2.17E-4 54 1.97E-4
19 SGTC_890 0929-0006 3.52 47.2 µM 2.37E-4 23 2.16E-4
20 SGTC_1031 k035-0009 3.50 16.0 µM 2.58E-4 46 2.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.167 1.71E-22 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.137 1.61E-15 YKL012W PRP40 het U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
0.126 2.78E-13 YMR317W_p YMR317W_p hom Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene
0.118 7.82E-12 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.115 2.35E-11 YBR153W RIB7 het Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
0.114 4.14E-11 YDL135C RDI1 hom Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
0.107 5.42E-10 YCR015C_p YCR015C_p hom Putative protein of unknown function; YCR015C is not an essential gene
0.105 9.97E-10 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.104 1.27E-9 YLR457C NBP1 het Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
0.104 1.32E-9 YFR014C CMK1 hom Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
0.099 9.42E-9 YPR042C PUF2 hom Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
0.092 9.03E-8 YDR169C-A_p YDR169C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.092 9.66E-8 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.089 2.23E-7 YGR082W TOM20 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.089 2.58E-7 YNR063W_p YNR063W_p hom Putative zinc-cluster protein of unknown function