YCR048W / ARE1

Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR048W / ARE1

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:4.05|P-value:1.23E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.10|P-value:7.95E-7 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.94|P-value:1.98E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:2.98|P-value:2.69E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:-3.31|P-value:5.20E-5 ID:SGTC_650|Compound:1328-0195|FD-Score:2.69|P-value:8.77E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.81|P-value:4.68E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.03|P-value:1.90E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.24|P-value:7.52E-5 ID:SGTC_959|Compound:1222-0044|FD-Score:3.44|P-value:3.08E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:3.80|P-value:4.99E-6 ID:SGTC_983|Compound:1491-0821|FD-Score:3.09|P-value:1.62E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:2.91|P-value:3.58E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.38|P-value:4.13E-5 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.10|P-value:1.43E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:-2.82|P-value:4.77E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.67|P-value:9.68E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-2.98|P-value:2.46E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-2.94|P-value:2.82E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.25|P-value:7.07E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:2.86|P-value:4.45E-4 ID:SGTC_1618|Compound:st003135|FD-Score:2.70|P-value:8.42E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-2.70|P-value:7.92E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.04|P-value:2.04E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-2.74|P-value:6.69E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.20|P-value:1.01E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-4.49|P-value:7.68E-8 ID:SGTC_2371|Compound:9071574|FD-Score:5.02|P-value:2.57E-9 ID:SGTC_2425|Compound:5628681|FD-Score:3.24|P-value:8.39E-5 ID:SGTC_2426|Compound:5582046|FD-Score:2.74|P-value:7.12E-4 ID:SGTC_2428|Compound:5615643|FD-Score:5.86|P-value:4.48E-12 ID:SGTC_2432|Compound:5630864|FD-Score:2.75|P-value:7.01E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.33|P-value:5.37E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:2.96|P-value:2.93E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.18|P-value:1.07E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.84|P-value:3.85E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.36|P-value:4.56E-5 ID:SGTC_3049|Compound:9092223|FD-Score:-3.30|P-value:5.64E-5 ID:SGTC_3164|Compound:9100705|FD-Score:-2.78|P-value:5.67E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.05|P-value:1.23E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.10|P-value:7.95E-7 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.94|P-value:1.98E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:2.98|P-value:2.69E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:-3.31|P-value:5.20E-5 ID:SGTC_650|Compound:1328-0195|FD-Score:2.69|P-value:8.77E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.81|P-value:4.68E-6 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.03|P-value:1.90E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.24|P-value:7.52E-5 ID:SGTC_959|Compound:1222-0044|FD-Score:3.44|P-value:3.08E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:3.80|P-value:4.99E-6 ID:SGTC_983|Compound:1491-0821|FD-Score:3.09|P-value:1.62E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:2.91|P-value:3.58E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.38|P-value:4.13E-5 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.10|P-value:1.43E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:-2.82|P-value:4.77E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.67|P-value:9.68E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-2.98|P-value:2.46E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-2.94|P-value:2.82E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.25|P-value:7.07E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:2.86|P-value:4.45E-4 ID:SGTC_1618|Compound:st003135|FD-Score:2.70|P-value:8.42E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-2.70|P-value:7.92E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.04|P-value:2.04E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-2.74|P-value:6.69E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.20|P-value:1.01E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-4.49|P-value:7.68E-8 ID:SGTC_2371|Compound:9071574|FD-Score:5.02|P-value:2.57E-9 ID:SGTC_2425|Compound:5628681|FD-Score:3.24|P-value:8.39E-5 ID:SGTC_2426|Compound:5582046|FD-Score:2.74|P-value:7.12E-4 ID:SGTC_2428|Compound:5615643|FD-Score:5.86|P-value:4.48E-12 ID:SGTC_2432|Compound:5630864|FD-Score:2.75|P-value:7.01E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.33|P-value:5.37E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:2.96|P-value:2.93E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.18|P-value:1.07E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.84|P-value:3.85E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.36|P-value:4.56E-5 ID:SGTC_3049|Compound:9092223|FD-Score:-3.30|P-value:5.64E-5 ID:SGTC_3164|Compound:9100705|FD-Score:-2.78|P-value:5.67E-4

Top fitness defect scores for YCR048W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2428 5615643 5.86 7.8 µM 4.48E-12 11 2.35E-9
2 SGTC_2371 9071574 5.02 200.0 µM 2.57E-9 4 2.61E-7
3 SGTC_103 0844-0104 4.05 51.8 µM 1.23E-6 ERAD & cell cycle 139 2.58E-5
4 SGTC_2752 diclazuril 3.84 33.1 µM 3.85E-6 128 6.04E-5
5 SGTC_745 0469-0705 3.81 296.0 µM 4.68E-6 mitochondrial processes 27 6.99E-5
6 SGTC_967 1306-0166 3.80 352.0 µM 4.99E-6 44 7.33E-5
7 SGTC_959 1222-0044 3.44 107.0 µM 3.08E-5 36 2.86E-4
8 SGTC_1298 1079-1278 3.38 263.0 µM 4.13E-5 DNA damage response 37 3.56E-4
9 SGTC_2923 7992654 3.36 58.1 µM 4.56E-5 Golgi 50 3.84E-4
10 SGTC_2546 rhodinyl acetate 3.33 100.0 µM 5.37E-5 27 4.34E-4
11 SGTC_2425 5628681 3.24 7.8 µM 8.39E-5 60 6.06E-4
12 SGTC_2119 5528445 3.20 120.6 µM 1.01E-4 46 6.99E-4
13 SGTC_2610 ononetin 3.18 44.2 µM 1.07E-4 83 7.25E-4
14 SGTC_983 1491-0821 3.09 12.2 µM 1.62E-4 37 9.96E-4
15 SGTC_1816 st051957 3.04 91.6 µM 2.04E-4 35 0.00118
16 SGTC_306 1672-3436 2.98 11.6 µM 2.69E-4 Golgi 50 0.00145
17 SGTC_2606 farnesol 2.96 9.8 µM 2.93E-4 74 0.00155
18 SGTC_1226 3909-7979 2.91 35.4 µM 3.58E-4 89 0.00180
19 SGTC_1597 7-benzyloxygramine 2.86 47.5 µM 4.45E-4 calcium & mitochondrial duress 247 0.00213
20 SGTC_2432 5630864 2.75 12.2 µM 7.01E-4 108 0.00300

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.92E-17 YDL239C ADY3 hom Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication
0.131 3.08E-14 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.123 8.18E-13 YBR256C RIB5 het Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
0.123 9.20E-13 YPL198W RPL7B hom Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
0.122 1.67E-12 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.121 2.28E-12 YKR039W GAP1 hom General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
0.119 4.44E-12 YLR204W QRI5 hom Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
0.114 4.07E-11 YMR276W DSK2 hom Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
0.111 1.26E-10 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.108 3.35E-10 YNR019W ARE2 hom Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
0.108 4.11E-10 YOR181W LAS17 het Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
0.103 2.43E-9 YJR099W YUH1 hom Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
0.100 6.36E-9 YAL051W OAF1 hom Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
0.099 7.98E-9 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.098 1.13E-8 YKR040C_d YKR040C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W