HIPHOP chemogenomics database

YCR053W / THR4

Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway

Zygosity: Homozygous strain

fixedexpanded
Profile for YCR053W / THR4

Click on Significant Values for Screen Details

Top fitness defect scores for YCR053W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	31.80	5.3 µM	1.55E-274		10	5.01E-222
2	SGTC_163	1327-0036	29.00	7.5 µM	7.48E-229		3	3.80E-185
3	SGTC_7	0192-1582	28.50	4.2 µM	3.27E-221		2	5.61E-179
4	SGTC_852	0418-0143	26.10	439.0 µM	3.28E-185	calcium & mitochondrial duress	1	6.51E-150
5	SGTC_2679	rapamycin	25.60	1.0 nM	4.50E-179		1	5.86E-145
6	SGTC_59	0330-0160	25.20	1.3 µM	6.56E-173		2	5.55E-140
7	SGTC_2651	1327-0036	21.80	5.0 µM	2.88E-130		1	1.49E-105
8	SGTC_386	1509-0011	18.50	3.1 µM	1.54E-94		10	1.05E-76
9	SGTC_310	1273-0058	16.30	1.1 µM	5.93E-74	mitochondrial stress	4	4.42E-60
10	SGTC_1635	st007035	14.70	42.4 µM	4.15E-60		42	6.72E-49
11	SGTC_3059	1327-0034	14.10	3.0 µM	7.60E-56		6	1.87E-45
12	SGTC_1844	rapamycin	12.50	4.0 nM	1.75E-44		19	2.82E-36
13	SGTC_3063	1327-0034	10.40	4.0 µM	1.91E-31		7	9.67E-26
14	SGTC_2430	5571206	9.45	200.0 µM	3.42E-26		9	1.71E-21
15	SGTC_177	1327-0068	8.81	8.4 µM	5.30E-23		11	6.49E-19
16	SGTC_3060	1327-0027	8.44	12.5 µM	2.73E-21		10	1.57E-17
17	SGTC_2233	sulbentine	8.21	40.4 µM	3.07E-20		12	1.12E-16
18	SGTC_18	0111-0177	6.77	500.0 nM	2.39E-14		12	6.53E-12
19	SGTC_513	4245-1575	5.99	41.7 µM	1.23E-11	unfolded protein response	46	1.03E-9
20	SGTC_64	000l-0018	5.68	272.1 µM	1.23E-10	ubiquinone biosynthesis & proteasome	87	6.66E-9

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.690	0	YER052C	HOM3	hom	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.503	1.12E-214	YHR025W	THR1	hom	Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.494	3.34E-205	YDR158W	HOM2	hom	Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.436	1.63E-155	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.422	1.07E-144	YBR068C	BAP2	hom	High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.420	3.40E-143	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.418	1.30E-141	YJR139C	HOM6	hom	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.412	1.16E-137	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.406	3.30E-133	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.404	1.06E-131	YGR208W	SER2	hom	Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.402	2.79E-130	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.402	4.46E-130	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.397	8.96E-127	YPL132W	COX11	hom	Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.388	1.98E-120	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.386	3.66E-119	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases