YCR084C / TUP1

General repressor of transcription, forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes

Zygosity: Homozygous strain
fixedexpanded
Profile for YCR084C / TUP1

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.24|P-value:4.38E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.04|P-value:9.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.30|P-value:3.53E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.90|P-value:3.16E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.19|P-value:5.36E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-4.35|P-value:4.07E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:-3.22|P-value:4.76E-4 ID:SGTC_1607|Compound:st000305|FD-Score:-3.52|P-value:1.52E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.06|P-value:8.26E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.77|P-value:5.66E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.37|P-value:2.64E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.04|P-value:8.89E-4 ID:SGTC_2031|Compound:5180455|FD-Score:-3.85|P-value:4.00E-5 ID:SGTC_2306|Compound:7482397|FD-Score:-4.10|P-value:1.29E-5 ID:SGTC_2851|Compound:9015922|FD-Score:3.07|P-value:8.11E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-4.30|P-value:5.20E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.14|P-value:6.33E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.13|P-value:6.39E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-4.42|P-value:2.95E-6 ID:SGTC_3200|Compound:9114385|FD-Score:3.37|P-value:2.63E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.37|P-value:2.70E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.24|P-value:4.38E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:-3.04|P-value:9.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.30|P-value:3.53E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.90|P-value:3.16E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.19|P-value:5.36E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-4.35|P-value:4.07E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:-3.22|P-value:4.76E-4 ID:SGTC_1607|Compound:st000305|FD-Score:-3.52|P-value:1.52E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.06|P-value:8.26E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.77|P-value:5.66E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.37|P-value:2.64E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.04|P-value:8.89E-4 ID:SGTC_2031|Compound:5180455|FD-Score:-3.85|P-value:4.00E-5 ID:SGTC_2306|Compound:7482397|FD-Score:-4.10|P-value:1.29E-5 ID:SGTC_2851|Compound:9015922|FD-Score:3.07|P-value:8.11E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-4.30|P-value:5.20E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.14|P-value:6.33E-4 ID:SGTC_3197|Compound:9113331|FD-Score:3.13|P-value:6.39E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-4.42|P-value:2.95E-6 ID:SGTC_3200|Compound:9114385|FD-Score:3.37|P-value:2.63E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.37|P-value:2.70E-4

Top fitness defect scores for YCR084C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_176 1486-1328 3.45 72.8 µM 1.97E-4 79 2.83E-4
2 SGTC_3200 9114385 3.37 49.5 µM 2.63E-4 64 3.72E-4
3 SGTC_1779 st039427 3.37 55.0 µM 2.64E-4 51 3.74E-4
4 SGTC_3281 9140613 3.37 49.5 µM 2.70E-4 RPP1 & pyrimidine depletion 38 3.81E-4
5 SGTC_23 0213-0011 3.24 40.0 µM 4.37E-4 copper-dependent oxidative stress 46 6.03E-4
6 SGTC_173 1273-0061 3.24 2.7 µM 4.38E-4 mitochondrial stress 220 6.05E-4
7 SGTC_3180 9109287 3.14 49.5 µM 6.33E-4 39 8.58E-4
8 SGTC_3197 9113331 3.13 49.5 µM 6.39E-4 78 8.66E-4
9 SGTC_2851 9015922 3.07 71.4 µM 8.11E-4 88 0.00109
10 SGTC_1651 st011928 3.06 86.1 µM 8.26E-4 RSC complex & mRNA processing 38 0.00111
11 SGTC_1942 st076330 3.04 65.2 µM 8.89E-4 67 0.00119
12 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 3.00 54.6 µM 0.00102 RPP1 & pyrimidine depletion 72 0.00135
13 SGTC_2378 9074213 3.00 121.4 µM 0.00102 RSC complex & mRNA processing 71 0.00135
14 SGTC_869 0564-0080 3.00 44.1 µM 0.00103 66 0.00136
15 SGTC_427 1354-0053 2.98 47.3 µM 0.00109 66 0.00144
16 SGTC_3234 9132280 2.98 49.5 µM 0.00109 65 0.00144
17 SGTC_184 1494-0393 2.94 24.7 µM 0.00125 120 0.00164
18 SGTC_1664 st014217 2.93 9.0 µM 0.00128 60S ribosome export 44 0.00168
19 SGTC_3186 9110464 2.93 49.5 µM 0.00129 RPP1 & pyrimidine depletion 42 0.00169
20 SGTC_1324 1418-0026 2.92 34.4 µM 0.00132 52 0.00173

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.21E-12 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.102 2.65E-9 YLR208W SEC13 het Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
0.088 2.87E-7 YLR225C_p YLR225C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene
0.085 7.65E-7 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.082 2.04E-6 YPL064C CWC27 hom Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
0.079 5.14E-6 YEL024W RIP1 hom Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
0.074 1.69E-5 YNL235C_d YNL235C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
0.074 1.70E-5 YBR204C LDH1 hom Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
0.071 3.82E-5 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.069 5.89E-5 YGR021W_p YGR021W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.069 6.20E-5 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.069 6.75E-5 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.069 7.14E-5 YGR204C-A_p YGR204C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.068 8.51E-5 YJR002W MPP10 het Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
0.067 9.59E-5 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase