Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YDL008W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.191 | 5.65E-29 | YML074C | FPR3 | hom | Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p |
0.174 | 2.88E-24 | YDL210W | UGA4 | hom | Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane |
0.163 | 2.36E-21 | YLR026C | SED5 | het | cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins |
0.161 | 7.48E-21 | YOR012W_p | YOR012W_p | hom | Putative protein of unknown function |
0.136 | 2.53E-15 | YGR246C | BRF1 | het | TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB |
0.127 | 1.91E-13 | YFR042W | KEG1 | het | Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability |
0.125 | 4.25E-13 | YBR262C | AIM5 | hom | Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture |
0.124 | 5.26E-13 | YEL049W | PAU2 | hom | Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme |
0.123 | 7.06E-13 | YPR026W | ATH1 | hom | Acid trehalase required for utilization of extracellular trehalose |
0.123 | 7.72E-13 | YML053C_p | YML053C_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene |
0.123 | 9.71E-13 | YKL131W_d | YKL131W_d | hom | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.123 | 9.96E-13 | YDR122W | KIN1 | hom | Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p |
0.120 | 2.57E-12 | YKR023W_p | YKR023W_p | hom | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
0.120 | 2.81E-12 | YNL042W | BOP3 | hom | Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury |
0.119 | 4.09E-12 | YHR122W | CIA2 | het | Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B |