HIPHOP chemogenomics database

YDL015C / TSC13

Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDL015C / TSC13

Click on Significant Values for Screen Details

Top fitness defect scores for YDL015C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2237	6667747	10.20	47.2 µM	5.81E-25		1	6.48E-25	4.04
2	SGTC_1632	st005650	5.85	90.0 µM	2.38E-9		1	2.39E-9	0.90
3	SGTC_2058	5236698	5.12	133.0 µM	1.51E-7		2	1.50E-7	1.23
4	SGTC_1629	st005085	5.10	44.9 µM	1.73E-7	RPP1 & pyrimidine depletion	9	1.72E-7	0.23
5	SGTC_1056	3448-1962	4.90	115.0 µM	4.76E-7	amide catabolism	2	4.72E-7	0.40
6	SGTC_1673	st016611	4.73	27.3 µM	1.14E-6		3	1.13E-6	0.38
7	SGTC_170	4197-0004	4.66	226.0 µM	1.58E-6		2	1.56E-6	0.64
8	SGTC_917	harmaline	4.62	728.1 nM	1.97E-6	ergosterol biosynthesis	5	1.95E-6	0.18
9	SGTC_2783	7738165	4.36	71.4 µM	6.71E-6		3	6.63E-6	0.38
10	SGTC_2238	6671620	3.91	73.2 µM	4.66E-5		5	4.59E-5	0.31
11	SGTC_225	nsc-122657	3.82	1.0 µM	6.87E-5		3	6.76E-5	0.18
12	SGTC_579	4491-1009	3.74	110.0 µM	9.41E-5	60S ribosome export	8	9.26E-5	0.10
13	SGTC_1159	4092-0538	3.62	61.2 µM	1.52E-4		9	1.50E-4	0.08
14	SGTC_362	0310-0100	3.56	9.0 µM	1.91E-4	RPP1 & pyrimidine depletion	12	1.88E-4	0.24
15	SGTC_1473	k844-0725	3.55	134.0 µM	1.93E-4		8	1.90E-4	0.06
16	SGTC_178	4092-0806	3.52	87.5 µM	2.19E-4	ergosterol depletion effects on membrane	28	2.15E-4	0.20
17	SGTC_708	3448-5400	3.46	146.0 µM	2.71E-4		30	2.66E-4	0.09
18	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	3.43	4.6 µM	3.08E-4	heat shock/prefoldin	27	3.03E-4	0.03
19	SGTC_2131	toluylene red	3.37	5.3 µM	3.87E-4	60S ribosome export	20	3.80E-4	0.12
20	SGTC_181	1447-1644	3.30	8.1 µM	4.94E-4	endomembrane recycling	17	4.85E-4	0.00

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.123	8.08E-13	YLR348C	DIC1	hom	Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
0.122	1.17E-12	YGL248W	PDE1	hom	Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
0.115	2.58E-11	YOR233W	KIN4	hom	Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.108	3.15E-10	YOR168W	GLN4	het	Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
0.103	2.34E-9	YIL045W	PIG2	hom	Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase
0.102	2.80E-9	YOL102C	TPT1	het	tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.092	8.30E-8	YDL161W	ENT1	hom	Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.090	1.79E-7	YNL243W	SLA2	hom	Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo
0.088	3.01E-7	YJR050W	ISY1	hom	Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
0.087	3.90E-7	YGR063C	SPT4	hom	Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
0.087	4.58E-7	YGR168C_p	YGR168C_p	hom	Putative protein of unknown function; YGR168C is not an essential gene
0.087	4.88E-7	YOL073C	YOL073C	hom	Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2
0.086	5.59E-7	YFR033C	QCR6	hom	Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
0.085	8.06E-7	YBR180W	DTR1	hom	Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation
0.085	9.24E-7	YDL133C-A	RPL41B	hom	Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication