YDL036C / PUS9

Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL036C / PUS9

Click on Significant Values for Screen Details ID:SGTC_316|Compound:k060-0003|FD-Score:3.38|P-value:1.34E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.13|P-value:3.63E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:-5.16|P-value:1.51E-8 ID:SGTC_668|Compound:1682-0499|FD-Score:-4.81|P-value:1.16E-7 ID:SGTC_669|Compound:0108-0021|FD-Score:2.91|P-value:8.41E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.78|P-value:2.35E-10 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.80|P-value:2.29E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.09|P-value:4.27E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.27|P-value:2.06E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.56|P-value:4.42E-7 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.78|P-value:2.21E-5 ID:SGTC_1291|Compound:0993-0025|FD-Score:-5.22|P-value:1.03E-8 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.08|P-value:4.68E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.29|P-value:2.08E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.59|P-value:5.75E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.09|P-value:4.25E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.78|P-value:2.29E-5 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.41|P-value:1.17E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-5.34|P-value:4.82E-9 ID:SGTC_2810|Compound:7959871|FD-Score:2.88|P-value:9.31E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-4.54|P-value:5.45E-7 ID:SGTC_316|Compound:k060-0003|FD-Score:3.38|P-value:1.34E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.13|P-value:3.63E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:-5.16|P-value:1.51E-8 ID:SGTC_668|Compound:1682-0499|FD-Score:-4.81|P-value:1.16E-7 ID:SGTC_669|Compound:0108-0021|FD-Score:2.91|P-value:8.41E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:5.78|P-value:2.35E-10 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.80|P-value:2.29E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.09|P-value:4.27E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.27|P-value:2.06E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.56|P-value:4.42E-7 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.78|P-value:2.21E-5 ID:SGTC_1291|Compound:0993-0025|FD-Score:-5.22|P-value:1.03E-8 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.08|P-value:4.68E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.29|P-value:2.08E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.59|P-value:5.75E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.09|P-value:4.25E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.78|P-value:2.29E-5 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.41|P-value:1.17E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-5.34|P-value:4.82E-9 ID:SGTC_2810|Compound:7959871|FD-Score:2.88|P-value:9.31E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-4.54|P-value:5.45E-7

Top fitness defect scores for YDL036C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_676 falcarinol 5.78 140.0 µM 2.35E-10 18 3.82E-9
2 SGTC_1283 0922-0125 4.56 16.4 µM 4.42E-7 13 2.60E-6
3 SGTC_1288 0973-0013 3.78 65.5 µM 2.21E-5 19 7.72E-5
4 SGTC_2326 6164455 3.78 177.6 µM 2.29E-5 mitochondrial processes 27 7.95E-5
5 SGTC_2675 theaflavin digallate 3.41 100.0 µM 1.17E-4 iron homeostasis 48 3.28E-4
6 SGTC_316 k060-0003 3.38 58.6 µM 1.34E-4 28 3.68E-4
7 SGTC_1281 0884-0031 3.27 11.8 µM 2.06E-4 fatty acid desaturase (OLE1) 51 5.36E-4
8 SGTC_391 gliotoxin 3.13 8.3 µM 3.63E-4 90 8.75E-4
9 SGTC_1529 apigenin 3.09 74.0 µM 4.25E-4 76 0.00100
10 SGTC_1239 0337-0584 3.09 35.4 µM 4.27E-4 unfolded protein response 100 0.00101
11 SGTC_669 0108-0021 2.91 26.2 µM 8.41E-4 fatty acid desaturase (OLE1) 160 0.00182
12 SGTC_2810 7959871 2.88 45.5 µM 9.31E-4 copper-dependent oxidative stress 104 0.00199
13 SGTC_6 0844-0013 2.83 19.8 µM 0.00113 242 0.00235
14 SGTC_689 0833-0039 2.79 98.0 µM 0.00130 ergosterol depletion effects on membrane 74 0.00266
15 SGTC_941 3237-0776 2.78 460.0 µM 0.00135 75 0.00275
16 SGTC_349 k015-0014 2.76 24.0 µM 0.00145 92 0.00292
17 SGTC_2755 lomerizine 2.74 42.7 µM 0.00153 100 0.00305
18 SGTC_963 1269-2853 2.70 151.0 µM 0.00178 90 0.00348
19 SGTC_3130 9127414 2.69 49.5 µM 0.00184 69 0.00359
20 SGTC_2593 nobiletin 2.66 86.2 µM 0.00206 98 0.00396

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.178 2.56E-25 YGR011W_d YGR011W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.147 1.01E-17 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.142 1.70E-16 YPL248C GAL4 hom DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p
0.128 1.18E-13 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.126 2.84E-13 YDR117C TMA64 hom Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
0.118 6.29E-12 YBR011C IPP1 het Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
0.114 3.75E-11 YDL027C_p YDL027C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.113 4.66E-11 YIL042C PKP1 hom Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p
0.113 4.88E-11 YJL132W_p YJL132W_p hom Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
0.110 1.63E-10 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.106 6.80E-10 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.102 3.39E-9 YPL060W MFM1 hom Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
0.101 5.15E-9 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.100 6.29E-9 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.100 6.63E-9 YLR355C ILV5 het Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids