YDL085W / NDE2

Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL085W / NDE2

Click on Significant Values for Screen Details ID:SGTC_13|Compound:0199-0087|FD-Score:-2.84|P-value:8.43E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.10|P-value:3.01E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:4.04|P-value:3.96E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.05|P-value:3.88E-6 ID:SGTC_121|Compound:0557-0495|FD-Score:2.82|P-value:9.18E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.78|P-value:1.46E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.11|P-value:2.91E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:4.25|P-value:1.33E-6 ID:SGTC_427|Compound:1354-0053|FD-Score:2.84|P-value:8.36E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.12|P-value:2.80E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.20|P-value:2.05E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.39|P-value:9.11E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.11|P-value:2.88E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:2.23E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:-4.01|P-value:4.63E-6 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.03|P-value:4.04E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.71|P-value:2.09E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.15|P-value:2.53E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.65|P-value:1.38E-7 ID:SGTC_1753|Compound:st043925|FD-Score:3.08|P-value:3.35E-4 ID:SGTC_1792|Compound:st045773|FD-Score:-3.49|P-value:5.61E-5 ID:SGTC_1943|Compound:st076331|FD-Score:-3.27|P-value:1.52E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.32|P-value:9.21E-7 ID:SGTC_2029|Compound:5175081|FD-Score:3.27|P-value:1.52E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.05|P-value:3.70E-4 ID:SGTC_2187|Compound:6125751|FD-Score:-2.87|P-value:7.58E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.64|P-value:2.94E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.17|P-value:1.99E-6 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-2.92|P-value:6.36E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.84|P-value:4.49E-8 ID:SGTC_2468|Compound:5987765|FD-Score:3.91|P-value:7.70E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.18|P-value:2.25E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.55|P-value:4.29E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:3.47|P-value:6.15E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.13|P-value:2.66E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-2.92|P-value:6.26E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.60|P-value:3.46E-5 ID:SGTC_2786|Compound:7743617|FD-Score:2.93|P-value:6.06E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.14|P-value:2.59E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.23|P-value:1.79E-4 ID:SGTC_3029|Compound:9088683|FD-Score:4.51|P-value:3.03E-7 ID:SGTC_3094|Compound:9117742|FD-Score:-3.35|P-value:1.09E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.09|P-value:3.26E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-3.46|P-value:6.52E-5 ID:SGTC_3122|Compound:9126309|FD-Score:-3.09|P-value:3.15E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-2.86|P-value:7.93E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:-2.84|P-value:8.43E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.10|P-value:3.01E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:4.04|P-value:3.96E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.05|P-value:3.88E-6 ID:SGTC_121|Compound:0557-0495|FD-Score:2.82|P-value:9.18E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.78|P-value:1.46E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.11|P-value:2.91E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:4.25|P-value:1.33E-6 ID:SGTC_427|Compound:1354-0053|FD-Score:2.84|P-value:8.36E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.12|P-value:2.80E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.20|P-value:2.05E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.39|P-value:9.11E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:-3.03|P-value:4.09E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.11|P-value:2.88E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:2.23E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:-4.01|P-value:4.63E-6 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.03|P-value:4.04E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.71|P-value:2.09E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.19|P-value:2.14E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.15|P-value:2.53E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.65|P-value:1.38E-7 ID:SGTC_1753|Compound:st043925|FD-Score:3.08|P-value:3.35E-4 ID:SGTC_1792|Compound:st045773|FD-Score:-3.49|P-value:5.61E-5 ID:SGTC_1943|Compound:st076331|FD-Score:-3.27|P-value:1.52E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.32|P-value:9.21E-7 ID:SGTC_2029|Compound:5175081|FD-Score:3.27|P-value:1.52E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.05|P-value:3.70E-4 ID:SGTC_2187|Compound:6125751|FD-Score:-2.87|P-value:7.58E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.64|P-value:2.94E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.17|P-value:1.99E-6 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-2.92|P-value:6.36E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.84|P-value:4.49E-8 ID:SGTC_2468|Compound:5987765|FD-Score:3.91|P-value:7.70E-6 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.18|P-value:2.25E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.55|P-value:4.29E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:3.47|P-value:6.15E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.13|P-value:2.66E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-2.92|P-value:6.26E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:3.60|P-value:3.46E-5 ID:SGTC_2786|Compound:7743617|FD-Score:2.93|P-value:6.06E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.14|P-value:2.59E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.23|P-value:1.79E-4 ID:SGTC_3029|Compound:9088683|FD-Score:4.51|P-value:3.03E-7 ID:SGTC_3094|Compound:9117742|FD-Score:-3.35|P-value:1.09E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.09|P-value:3.26E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-3.46|P-value:6.52E-5 ID:SGTC_3122|Compound:9126309|FD-Score:-3.09|P-value:3.15E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-2.86|P-value:7.93E-4

Top fitness defect scores for YDL085W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2419 paclitaxel 4.84 166.5 µM 4.49E-8 17 6.61E-7
2 SGTC_1712 st032288 4.65 53.4 µM 1.38E-7 calcium & mitochondrial duress 135 1.66E-6
3 SGTC_3029 9088683 4.51 49.5 µM 3.03E-7 16 3.18E-6
4 SGTC_365 0121-0052 4.25 13.6 µM 1.33E-6 mitochondrial response to ROS 47 1.08E-5
5 SGTC_2301 7769476 4.17 200.0 µM 1.99E-6 21 1.51E-5
6 SGTC_102 gallobenzophenone 4.05 83.6 µM 3.88E-6 redox potentiating 41 2.61E-5
7 SGTC_95 0471-0001 4.04 561.1 µM 3.96E-6 24 2.66E-5
8 SGTC_2468 5987765 3.91 72.9 µM 7.70E-6 16 4.60E-5
9 SGTC_174 3553-0389 3.78 74.3 µM 1.46E-5 25 7.80E-5
10 SGTC_1230 0170-0365 3.71 3.8 µM 2.09E-5 endomembrane recycling 80 1.05E-4
11 SGTC_2736 butenafine 3.60 14.7 µM 3.46E-5 39 1.60E-4
12 SGTC_2569 tetrandrine 3.55 100.0 µM 4.29E-5 35 1.90E-4
13 SGTC_2576 guaiol 3.47 23.1 µM 6.15E-5 41 2.57E-4
14 SGTC_873 0591-4978 3.39 488.0 µM 9.11E-5 28 3.55E-4
15 SGTC_2029 5175081 3.27 200.0 µM 1.52E-4 45 5.42E-4
16 SGTC_2930 9013230 3.23 14.9 µM 1.79E-4 heme biosynthesis & mitochondrial translocase 73 6.20E-4
17 SGTC_528 3492-0019 3.20 172.0 µM 2.05E-4 calcium & mitochondrial duress 286 6.95E-4
18 SGTC_1359 1582-0056 3.15 520.0 nM 2.53E-4 51 8.29E-4
19 SGTC_2821 7996764 3.14 71.4 µM 2.59E-4 81 8.44E-4
20 SGTC_2693 st077581 3.13 40.7 µM 2.66E-4 81 8.63E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 6.70E-27 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.182 2.36E-26 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.174 2.78E-24 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.155 1.47E-19 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.150 1.93E-18 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.149 3.56E-18 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.124 5.47E-13 YGR128C UTP8 het Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.117 1.03E-11 YDL133C-A RPL41B hom Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
0.116 1.47E-11 YGL263W COS12 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.115 2.31E-11 YLR228C ECM22 hom Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
0.113 4.83E-11 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.113 6.09E-11 YDR491C_d YDR491C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.112 8.95E-11 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.111 1.38E-10 YGR237C_p YGR237C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.110 1.47E-10 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability