YDL125C / HNT1

Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL125C / HNT1

Click on Significant Values for Screen Details ID:SGTC_5|Compound:0352-0601|FD-Score:-4.07|P-value:7.09E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.20|P-value:9.66E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.56|P-value:4.56E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:5.34|P-value:1.47E-7 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.49|P-value:3.73E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.01|P-value:7.40E-7 ID:SGTC_793|Compound:0488-0545|FD-Score:6.30|P-value:7.94E-10 ID:SGTC_811|Compound:0416-0114|FD-Score:-3.37|P-value:8.43E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-4.33|P-value:2.54E-5 ID:SGTC_950|Compound:1165-0508|FD-Score:3.54|P-value:3.12E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.21|P-value:9.39E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.28|P-value:7.50E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.39|P-value:5.33E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.79|P-value:2.07E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.71|P-value:1.72E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.82|P-value:1.14E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.35|P-value:6.05E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.07|P-value:4.42E-5 ID:SGTC_1623|Compound:st003709|FD-Score:-3.51|P-value:5.32E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.48|P-value:3.81E-4 ID:SGTC_1741|Compound:st038134|FD-Score:-3.50|P-value:5.50E-4 ID:SGTC_1744|Compound:st037937|FD-Score:3.32|P-value:6.72E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-4.85|P-value:2.73E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.56|P-value:2.98E-4 ID:SGTC_2067|Compound:5224629|FD-Score:-4.03|P-value:8.48E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.85|P-value:1.04E-4 ID:SGTC_2151|Compound:5563267|FD-Score:4.23|P-value:2.29E-5 ID:SGTC_2165|Compound:5727197|FD-Score:-8.08|P-value:1.44E-14 ID:SGTC_2210|Compound:7115885|FD-Score:3.20|P-value:9.74E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.11|P-value:3.71E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.67|P-value:3.61E-6 ID:SGTC_2426|Compound:5582046|FD-Score:3.29|P-value:7.32E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.51|P-value:3.50E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.21|P-value:9.35E-4 ID:SGTC_2835|Compound:9006588|FD-Score:3.63|P-value:2.26E-4 ID:SGTC_2894|Compound:9048625|FD-Score:-3.51|P-value:5.41E-4 ID:SGTC_2981|Compound:9013952|FD-Score:3.35|P-value:5.98E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.93|P-value:7.44E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.18|P-value:2.85E-5 ID:SGTC_3117|Compound:9124945|FD-Score:3.72|P-value:1.67E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.76|P-value:2.35E-6 ID:SGTC_3252|Compound:9135871|FD-Score:-3.35|P-value:9.18E-4 ID:SGTC_5|Compound:0352-0601|FD-Score:-4.07|P-value:7.09E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.20|P-value:9.66E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.56|P-value:4.56E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:5.34|P-value:1.47E-7 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.49|P-value:3.73E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.01|P-value:7.40E-7 ID:SGTC_793|Compound:0488-0545|FD-Score:6.30|P-value:7.94E-10 ID:SGTC_811|Compound:0416-0114|FD-Score:-3.37|P-value:8.43E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-4.33|P-value:2.54E-5 ID:SGTC_950|Compound:1165-0508|FD-Score:3.54|P-value:3.12E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.21|P-value:9.39E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.28|P-value:7.50E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.39|P-value:5.33E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.79|P-value:2.07E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.71|P-value:1.72E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.82|P-value:1.14E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.35|P-value:6.05E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:4.07|P-value:4.42E-5 ID:SGTC_1623|Compound:st003709|FD-Score:-3.51|P-value:5.32E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.48|P-value:3.81E-4 ID:SGTC_1741|Compound:st038134|FD-Score:-3.50|P-value:5.50E-4 ID:SGTC_1744|Compound:st037937|FD-Score:3.32|P-value:6.72E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-4.85|P-value:2.73E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.56|P-value:2.98E-4 ID:SGTC_2067|Compound:5224629|FD-Score:-4.03|P-value:8.48E-5 ID:SGTC_2137|Compound:5261020|FD-Score:3.85|P-value:1.04E-4 ID:SGTC_2151|Compound:5563267|FD-Score:4.23|P-value:2.29E-5 ID:SGTC_2165|Compound:5727197|FD-Score:-8.08|P-value:1.44E-14 ID:SGTC_2210|Compound:7115885|FD-Score:3.20|P-value:9.74E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.11|P-value:3.71E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.67|P-value:3.61E-6 ID:SGTC_2426|Compound:5582046|FD-Score:3.29|P-value:7.32E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.51|P-value:3.50E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.21|P-value:9.35E-4 ID:SGTC_2835|Compound:9006588|FD-Score:3.63|P-value:2.26E-4 ID:SGTC_2894|Compound:9048625|FD-Score:-3.51|P-value:5.41E-4 ID:SGTC_2981|Compound:9013952|FD-Score:3.35|P-value:5.98E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.93|P-value:7.44E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.18|P-value:2.85E-5 ID:SGTC_3117|Compound:9124945|FD-Score:3.72|P-value:1.67E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.76|P-value:2.35E-6 ID:SGTC_3252|Compound:9135871|FD-Score:-3.35|P-value:9.18E-4

Top fitness defect scores for YDL125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_793 0488-0545 6.30 106.0 µM 7.94E-10 1 1.51E-10
2 SGTC_121 0557-0495 5.34 61.3 µM 1.47E-7 calcium & mitochondrial duress 23 4.64E-8
3 SGTC_514 4466-0038 5.01 2.6 nM 7.40E-7 cell wall 106 2.71E-7
4 SGTC_1243 0416-0003 4.79 5.7 µM 2.07E-6 PDR1 11 8.38E-7
5 SGTC_3157 9099274 4.76 49.5 µM 2.35E-6 9 9.58E-7
6 SGTC_2405 ticlatone 4.67 3.5 µM 3.61E-6 44 1.54E-6
7 SGTC_2151 5563267 4.23 85.4 µM 2.29E-5 16 1.16E-5
8 SGTC_3059 1327-0034 4.18 3.0 µM 2.85E-5 47 1.46E-5
9 SGTC_2402 5553605 4.11 200.0 µM 3.71E-5 20 1.95E-5
10 SGTC_1511 0906-3109 4.07 28.5 µM 4.42E-5 excess fatty acid 41 2.36E-5
11 SGTC_3010 9080089 3.93 71.4 µM 7.44E-5 13 4.16E-5
12 SGTC_2137 5261020 3.85 140.0 µM 1.04E-4 40 6.01E-5
13 SGTC_1345 1488-0192 3.82 31.4 µM 1.14E-4 19 6.62E-5
14 SGTC_3117 9124945 3.72 49.5 µM 1.67E-4 8 1.00E-4
15 SGTC_1258 0671-0043 3.71 57.1 µM 1.72E-4 31 1.04E-4
16 SGTC_2835 9006588 3.63 71.4 µM 2.26E-4 54 1.40E-4
17 SGTC_2012 4023501 3.56 200.0 µM 2.98E-4 45 1.89E-4
18 SGTC_950 1165-0508 3.54 94.1 µM 3.12E-4 41 1.99E-4
19 SGTC_2638 epoxomicin 3.51 18.0 µM 3.50E-4 48 2.25E-4
20 SGTC_402 epigallocatechin gallate 3.49 10.0 µM 3.73E-4 44 2.40E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.135 5.13E-15 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.098 1.28E-8 YNR027W BUD17 hom Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
0.096 2.58E-8 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.094 5.00E-8 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.092 8.13E-8 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.089 2.08E-7 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.088 3.77E-7 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.087 4.49E-7 YOR343C_d YOR343C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.083 1.64E-6 YNL172W APC1 het Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
0.079 5.09E-6 YPR086W SUA7 het Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
0.078 6.81E-6 YNL260C LTO1 het Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.077 7.90E-6 YDL236W PHO13 hom Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity
0.077 8.53E-6 YDR139C RUB1 hom Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
0.076 9.41E-6 YFR031C-A RPL2A hom Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
0.076 9.70E-6 YDL138W RGT2 hom Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication