YDL128W / VCX1

Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL128W / VCX1

Click on Significant Values for Screen Details ID:SGTC_136|Compound:0097-0015|FD-Score:-2.83|P-value:4.97E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-2.77|P-value:6.50E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.08|P-value:1.68E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.70|P-value:8.56E-6 ID:SGTC_458|Compound:nppb|FD-Score:3.66|P-value:1.08E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:2.85|P-value:4.71E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-2.95|P-value:3.09E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.76|P-value:6.29E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:2.88|P-value:4.12E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.22|P-value:9.23E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:2.85|P-value:4.71E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:2.92|P-value:3.43E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:2.82|P-value:5.26E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.15|P-value:1.11E-9 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.22|P-value:9.02E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.16|P-value:1.20E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:2.82|P-value:5.38E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:4.32|P-value:2.59E-7 ID:SGTC_1463|Compound:k081-0032|FD-Score:-2.93|P-value:3.28E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.17|P-value:1.14E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.60|P-value:1.46E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-2.79|P-value:5.95E-4 ID:SGTC_1634|Compound:st006576|FD-Score:2.83|P-value:4.98E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.27|P-value:3.38E-7 ID:SGTC_1721|Compound:st082152|FD-Score:2.74|P-value:7.18E-4 ID:SGTC_1741|Compound:st038134|FD-Score:3.40|P-value:3.98E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.03|P-value:2.14E-4 ID:SGTC_2138|Compound:5575593|FD-Score:-2.72|P-value:7.77E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-2.78|P-value:6.19E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.64|P-value:1.19E-5 ID:SGTC_2320|Compound:7373003|FD-Score:-2.93|P-value:3.30E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-2.83|P-value:5.02E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.05|P-value:1.95E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.23|P-value:8.61E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-2.74|P-value:7.11E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-2.90|P-value:3.67E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.36|P-value:4.76E-5 ID:SGTC_3310|Compound:9130229|FD-Score:4.21|P-value:5.00E-7 ID:SGTC_136|Compound:0097-0015|FD-Score:-2.83|P-value:4.97E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-2.77|P-value:6.50E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.08|P-value:1.68E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.70|P-value:8.56E-6 ID:SGTC_458|Compound:nppb|FD-Score:3.66|P-value:1.08E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:2.85|P-value:4.71E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:-2.95|P-value:3.09E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.76|P-value:6.29E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:2.88|P-value:4.12E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.22|P-value:9.23E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:2.85|P-value:4.71E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:2.92|P-value:3.43E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:2.82|P-value:5.26E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.15|P-value:1.11E-9 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.22|P-value:9.02E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.16|P-value:1.20E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:2.82|P-value:5.38E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:4.32|P-value:2.59E-7 ID:SGTC_1463|Compound:k081-0032|FD-Score:-2.93|P-value:3.28E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.17|P-value:1.14E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.60|P-value:1.46E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-2.79|P-value:5.95E-4 ID:SGTC_1634|Compound:st006576|FD-Score:2.83|P-value:4.98E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-4.27|P-value:3.38E-7 ID:SGTC_1721|Compound:st082152|FD-Score:2.74|P-value:7.18E-4 ID:SGTC_1741|Compound:st038134|FD-Score:3.40|P-value:3.98E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.03|P-value:2.14E-4 ID:SGTC_2138|Compound:5575593|FD-Score:-2.72|P-value:7.77E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-2.78|P-value:6.19E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.64|P-value:1.19E-5 ID:SGTC_2320|Compound:7373003|FD-Score:-2.93|P-value:3.30E-4 ID:SGTC_2325|Compound:6158266|FD-Score:-2.83|P-value:5.02E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.05|P-value:1.95E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.23|P-value:8.61E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-2.74|P-value:7.11E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-2.90|P-value:3.67E-4 ID:SGTC_3303|Compound:9125618|FD-Score:3.36|P-value:4.76E-5 ID:SGTC_3310|Compound:9130229|FD-Score:4.21|P-value:5.00E-7

Top fitness defect scores for YDL128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1230 0170-0365 5.15 3.8 µM 1.11E-9 endomembrane recycling 35 1.32E-7
2 SGTC_1434 4073-0095 4.32 163.0 µM 2.59E-7 8 7.76E-6
3 SGTC_3310 9130229 4.21 44.1 µM 5.00E-7 Golgi 18 1.27E-5
4 SGTC_542 3381-0244 3.76 89.3 µM 6.29E-6 45 8.50E-5
5 SGTC_159 3970-0959 3.70 50.0 µM 8.56E-6 91 1.07E-4
6 SGTC_458 nppb 3.66 133.0 µM 1.08E-5 19 1.27E-4
7 SGTC_2270 7924575 3.64 90.9 µM 1.19E-5 RPP1 & pyrimidine depletion 22 1.38E-4
8 SGTC_1490 4089-0339 3.60 670.0 nM 1.46E-5 13 1.60E-4
9 SGTC_1741 st038134 3.40 10.2 µM 3.98E-5 50 3.41E-4
10 SGTC_3303 9125618 3.36 40.6 µM 4.76E-5 68 3.90E-4
11 SGTC_2808 7946468 3.23 45.5 µM 8.61E-5 59 6.10E-4
12 SGTC_1343 1487-0095 3.22 224.0 µM 9.02E-5 24 6.31E-4
13 SGTC_858 0438-0351 3.22 2.8 µM 9.23E-5 copper-dependent oxidative stress 46 6.43E-4
14 SGTC_1404 3909-7820 3.16 48.3 µM 1.20E-4 60S ribosome export 30 7.83E-4
15 SGTC_1818 st052136 3.03 56.3 µM 2.14E-4 NEO1-PIK1 105 0.00121
16 SGTC_913 3487-1245 2.92 167.0 µM 3.43E-4 92 0.00173
17 SGTC_545 4218-0007 2.88 221.0 µM 4.12E-4 71 0.00199
18 SGTC_862 0452-0056 2.85 10.2 µM 4.71E-4 azole & statin 77 0.00220
19 SGTC_523 1934-0222 2.85 13.7 µM 4.71E-4 84 0.00220
20 SGTC_1634 st006576 2.83 41.0 µM 4.98E-4 TSC3-RPN4 76 0.00230

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 1.43E-31 YDR501W PLM2 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
0.173 4.31E-24 YHR001W OSH7 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.173 4.55E-24 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.146 2.02E-17 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.127 1.72E-13 YEL047C FRD1 hom Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
0.125 4.17E-13 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.123 8.05E-13 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.116 1.48E-11 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.110 1.85E-10 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.107 4.80E-10 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.101 5.04E-9 YDL218W_p YDL218W_p hom Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
0.100 7.10E-9 YGR072W UPF3 hom Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
0.097 1.66E-8 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.096 2.85E-8 YDR274C_d YDR274C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 6.56E-8 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication