Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
Profile for YDL129W / YDL129W

Click on Significant Values for Screen Details

Top fitness defect scores for YDL129W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_2563 tomatine 4.26 8.5 µM 4.04E-7 33 1.04E-5
2 SGTC_1523 st012844 4.19 44.7 µM 5.97E-7 60S ribosome export 14 1.40E-5
3 SGTC_151 3013-0144 4.05 2.3 µM 1.34E-6 iron homeostasis 25 2.58E-5
4 SGTC_3330 9141424 3.93 71.4 µM 2.60E-6 amide catabolism 19 4.26E-5
5 SGTC_1468 k284-1086 3.87 134.0 µM 3.63E-6 DNA intercalators 40 5.49E-5
6 SGTC_955 1216-0110 3.78 33.0 µM 5.73E-6 sphingolipid biosynthesis & PDR1 77 7.77E-5
7 SGTC_1094 duloxetine 3.56 25.4 µM 1.81E-5 RPP1 & pyrimidine depletion 31 1.86E-4
8 SGTC_2909 7941780 3.49 10.5 µM 2.55E-5 35 2.41E-4
9 SGTC_628 1636-0253 3.26 16.0 µM 7.62E-5 sphingolipid biosynthesis & PDR1 59 5.56E-4
10 SGTC_1129 3453-1384 3.25 223.0 µM 8.08E-5 72 5.81E-4
11 SGTC_874 0599-0792 3.17 117.0 µM 1.18E-4 66 7.75E-4
12 SGTC_1113 artemisinin 3.06 1.2 µM 1.88E-4 heme biosynthesis & mitochondrial translocase 41 0.00110
13 SGTC_1741 st038134 3.01 10.2 µM 2.35E-4 78 0.00131
14 SGTC_3323 9138564 2.97 53.9 µM 2.79E-4 64 0.00149
15 SGTC_2230 bromochlorosalicylanilide 2.89 954.5 nM 3.84E-4 24 0.00191
16 SGTC_361 000s-0510 2.87 1.4 µM 4.31E-4 101 0.00208
17 SGTC_889 0919-1524 2.85 82.7 µM 4.58E-4 55 0.00218
18 SGTC_1620 st003704 2.85 16.5 µM 4.62E-4 53 0.00220
19 SGTC_1740 st038286 2.80 13.2 µM 5.57E-4 98 0.00253
20 SGTC_2736 butenafine 2.77 14.7 µM 6.35E-4 93 0.00280

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.85E-13 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.115 2.60E-11 YBR101C FES1 hom Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.111 1.05E-10 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.101 4.87E-9 YPR186C PZF1 het Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
0.101 5.34E-9 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.096 2.84E-8 YEL038W UTR4 hom Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus
0.095 3.13E-8 YJL118W YJL118W hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
0.093 7.21E-8 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.089 2.27E-7 YHR107C CDC12 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.087 4.03E-7 YNL212W VID27 hom Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
0.084 1.26E-6 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.084 1.27E-6 YGR249W MGA1 hom Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
0.081 2.91E-6 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.080 4.16E-6 YBL065W_d YBL065W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
0.079 4.13E-6 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall