YDL134C / PPH21

Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL134C / PPH21

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:2.85|P-value:8.65E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.38|P-value:8.46E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:2.84|P-value:8.99E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.73|P-value:2.16E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:4.70|P-value:1.28E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.02|P-value:6.78E-15 ID:SGTC_514|Compound:4466-0038|FD-Score:4.10|P-value:3.40E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:2.83|P-value:9.41E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.37|P-value:1.11E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.35|P-value:1.24E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:-2.92|P-value:7.00E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.30|P-value:1.16E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.48|P-value:6.57E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.63|P-value:3.44E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.96|P-value:6.83E-6 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.02|P-value:4.61E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:2.83|P-value:9.32E-4 ID:SGTC_1713|Compound:st033146|FD-Score:3.07|P-value:3.82E-4 ID:SGTC_1783|Compound:st039459|FD-Score:-5.38|P-value:1.95E-9 ID:SGTC_1784|Compound:st039334|FD-Score:9.35|P-value:5.81E-25 ID:SGTC_1800|Compound:st050079|FD-Score:3.01|P-value:4.81E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.46|P-value:7.33E-5 ID:SGTC_1996|Compound:st072960|FD-Score:3.40|P-value:9.52E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.10|P-value:3.38E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.33|P-value:1.37E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.01|P-value:4.94E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.49|P-value:6.48E-5 ID:SGTC_2395|Compound:5646831|FD-Score:2.83|P-value:9.61E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.05|P-value:4.12E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.01|P-value:4.94E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.14|P-value:2.95E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.87|P-value:4.72E-8 ID:SGTC_2824|Compound:7998404|FD-Score:3.40|P-value:9.55E-5 ID:SGTC_2825|Compound:7998094|FD-Score:-3.01|P-value:4.95E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.12|P-value:3.09E-4 ID:SGTC_3026|Compound:9090633|FD-Score:-3.47|P-value:7.44E-5 ID:SGTC_3188|Compound:9110789|FD-Score:3.47|P-value:7.02E-5 ID:SGTC_3194|Compound:9111903|FD-Score:3.36|P-value:1.12E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.14|P-value:2.81E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.12|P-value:3.09E-4 ID:SGTC_3311|Compound:9131146|FD-Score:2.94|P-value:6.15E-4 ID:SGTC_3316|Compound:9136639|FD-Score:-3.50|P-value:6.39E-5 ID:SGTC_3317|Compound:9138495|FD-Score:-3.54|P-value:5.48E-5 ID:SGTC_45|Compound:0kpi-0036|FD-Score:2.85|P-value:8.65E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.38|P-value:8.46E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:2.84|P-value:8.99E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.73|P-value:2.16E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:4.70|P-value:1.28E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.02|P-value:6.78E-15 ID:SGTC_514|Compound:4466-0038|FD-Score:4.10|P-value:3.40E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:2.83|P-value:9.41E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.37|P-value:1.11E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.35|P-value:1.24E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:-2.92|P-value:7.00E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.30|P-value:1.16E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.48|P-value:6.57E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.63|P-value:3.44E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.96|P-value:6.83E-6 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.02|P-value:4.61E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:2.83|P-value:9.32E-4 ID:SGTC_1713|Compound:st033146|FD-Score:3.07|P-value:3.82E-4 ID:SGTC_1783|Compound:st039459|FD-Score:-5.38|P-value:1.95E-9 ID:SGTC_1784|Compound:st039334|FD-Score:9.35|P-value:5.81E-25 ID:SGTC_1800|Compound:st050079|FD-Score:3.01|P-value:4.81E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.46|P-value:7.33E-5 ID:SGTC_1996|Compound:st072960|FD-Score:3.40|P-value:9.52E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.10|P-value:3.38E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.33|P-value:1.37E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.01|P-value:4.94E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.49|P-value:6.48E-5 ID:SGTC_2395|Compound:5646831|FD-Score:2.83|P-value:9.61E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.05|P-value:4.12E-4 ID:SGTC_2725|Compound:haloperidol|FD-Score:-3.01|P-value:4.94E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.14|P-value:2.95E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.87|P-value:4.72E-8 ID:SGTC_2824|Compound:7998404|FD-Score:3.40|P-value:9.55E-5 ID:SGTC_2825|Compound:7998094|FD-Score:-3.01|P-value:4.95E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.12|P-value:3.09E-4 ID:SGTC_3026|Compound:9090633|FD-Score:-3.47|P-value:7.44E-5 ID:SGTC_3188|Compound:9110789|FD-Score:3.47|P-value:7.02E-5 ID:SGTC_3194|Compound:9111903|FD-Score:3.36|P-value:1.12E-4 ID:SGTC_3209|Compound:9113616|FD-Score:3.14|P-value:2.81E-4 ID:SGTC_3292|Compound:9120825|FD-Score:3.12|P-value:3.09E-4 ID:SGTC_3311|Compound:9131146|FD-Score:2.94|P-value:6.15E-4 ID:SGTC_3316|Compound:9136639|FD-Score:-3.50|P-value:6.39E-5 ID:SGTC_3317|Compound:9138495|FD-Score:-3.54|P-value:5.48E-5

Top fitness defect scores for YDL134C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1784 st039334 9.35 36.3 µM 5.81E-25 2 4.31E-21
2 SGTC_453 enantio-paf c16 7.02 10.0 µM 6.78E-15 25 1.09E-12
3 SGTC_2809 7964133 4.87 13.0 µM 4.72E-8 60S ribosome export 17 5.70E-7
4 SGTC_435 c-PAF 4.70 6.7 µM 1.28E-7 19 1.31E-6
5 SGTC_1192 1488-0095 4.30 21.4 µM 1.16E-6 11 8.35E-6
6 SGTC_514 4466-0038 4.10 2.6 nM 3.40E-6 cell wall 142 2.06E-5
7 SGTC_1257 0669-0121 3.96 16.0 µM 6.83E-6 26 3.69E-5
8 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 3.73 8.0 µM 2.16E-5 32 9.70E-5
9 SGTC_1247 0448-0043 3.63 5.8 µM 3.44E-5 ERG2 62 1.43E-4
10 SGTC_2394 5221648 3.49 200.0 µM 6.48E-5 36 2.44E-4
11 SGTC_1226 3909-7979 3.48 35.4 µM 6.57E-5 58 2.47E-4
12 SGTC_3188 9110789 3.47 49.5 µM 7.02E-5 iron homeostasis 39 2.61E-4
13 SGTC_1809 st050081 3.46 57.6 µM 7.33E-5 28 2.71E-4
14 SGTC_1996 st072960 3.40 66.8 µM 9.52E-5 36 3.37E-4
15 SGTC_2824 7998404 3.40 52.0 µM 9.55E-5 50 3.38E-4
16 SGTC_1032 k061-1018 3.37 98.7 µM 1.11E-4 45 3.82E-4
17 SGTC_3194 9111903 3.36 49.5 µM 1.12E-4 43 3.88E-4
18 SGTC_3209 9113616 3.14 49.5 µM 2.81E-4 69 8.39E-4
19 SGTC_3292 9120825 3.12 59.8 µM 3.09E-4 39 9.07E-4
20 SGTC_2975 9084847 3.12 53.7 µM 3.09E-4 67 9.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.187 1.04E-27 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.175 1.57E-24 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.170 3.19E-23 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.152 7.58E-19 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.147 1.14E-17 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.145 3.36E-17 YHR155W YSP1 hom Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
0.144 5.14E-17 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
0.144 5.11E-17 YDL188C PPH22 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
0.141 1.95E-16 YER139C RTR1 hom CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
0.140 3.48E-16 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.137 1.47E-15 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.137 1.83E-15 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.128 8.50E-14 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.125 4.34E-13 YPL150W_p YPL150W_p hom Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
0.124 4.72E-13 YHR204W MNL1 hom Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation