YDL146W / LDB17

Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL146W / LDB17

Click on Significant Values for Screen Details ID:SGTC_149|Compound:3970-0822|FD-Score:6.29|P-value:1.35E-12 ID:SGTC_150|Compound:3970-0969|FD-Score:3.03|P-value:3.80E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.35|P-value:6.45E-7 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.88|P-value:2.81E-8 ID:SGTC_601|Compound:0986-0029|FD-Score:2.80|P-value:9.27E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:2.91|P-value:6.17E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:2.88|P-value:6.85E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:2.80|P-value:9.29E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:4.64|P-value:1.21E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:2.81|P-value:8.88E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.05|P-value:3.44E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.88|P-value:7.91E-6 ID:SGTC_1105|Compound:epirubicin|FD-Score:-2.79|P-value:9.05E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.98|P-value:4.48E-6 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.39|P-value:7.79E-5 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.07|P-value:3.26E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:2.79|P-value:9.54E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.92|P-value:5.54E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.57|P-value:1.67E-7 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.67|P-value:2.10E-5 ID:SGTC_1409|Compound:3909-8004|FD-Score:2.88|P-value:6.99E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.15|P-value:2.37E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.34|P-value:9.50E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:2.83|P-value:8.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.34|P-value:1.01E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-2.81|P-value:8.49E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.38|P-value:8.74E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.61|P-value:3.02E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:2.83|P-value:8.32E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.74|P-value:6.19E-8 ID:SGTC_1934|Compound:st074720|FD-Score:-4.29|P-value:8.44E-7 ID:SGTC_1937|Compound:st074714|FD-Score:-3.09|P-value:2.83E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.03|P-value:3.87E-4 ID:SGTC_2085|Compound:5307070|FD-Score:-3.81|P-value:1.07E-5 ID:SGTC_2086|Compound:5274770|FD-Score:-3.10|P-value:2.71E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.94|P-value:5.38E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.00|P-value:4.04E-4 ID:SGTC_2423|Compound:st077119|FD-Score:-3.45|P-value:5.77E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.00|P-value:3.98E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.37|P-value:5.93E-7 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.33|P-value:1.07E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.64|P-value:2.38E-5 ID:SGTC_2955|Compound:9076254|FD-Score:4.43|P-value:4.32E-7 ID:SGTC_3013|Compound:9080133|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.37|P-value:8.63E-5 ID:SGTC_3318|Compound:9139014|FD-Score:-3.03|P-value:3.66E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:6.29|P-value:1.35E-12 ID:SGTC_150|Compound:3970-0969|FD-Score:3.03|P-value:3.80E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.35|P-value:6.45E-7 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.88|P-value:2.81E-8 ID:SGTC_601|Compound:0986-0029|FD-Score:2.80|P-value:9.27E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:2.91|P-value:6.17E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:2.88|P-value:6.85E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:2.80|P-value:9.29E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:4.64|P-value:1.21E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:2.81|P-value:8.88E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.05|P-value:3.44E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.88|P-value:7.91E-6 ID:SGTC_1105|Compound:epirubicin|FD-Score:-2.79|P-value:9.05E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.98|P-value:4.48E-6 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.39|P-value:7.79E-5 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.07|P-value:3.26E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:2.79|P-value:9.54E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.92|P-value:5.54E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.57|P-value:1.67E-7 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.67|P-value:2.10E-5 ID:SGTC_1409|Compound:3909-8004|FD-Score:2.88|P-value:6.99E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.15|P-value:2.37E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.34|P-value:9.50E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:2.83|P-value:8.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.06|P-value:3.37E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.34|P-value:1.01E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-2.81|P-value:8.49E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.38|P-value:8.74E-5 ID:SGTC_1800|Compound:st050079|FD-Score:3.61|P-value:3.02E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:2.83|P-value:8.32E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.74|P-value:6.19E-8 ID:SGTC_1934|Compound:st074720|FD-Score:-4.29|P-value:8.44E-7 ID:SGTC_1937|Compound:st074714|FD-Score:-3.09|P-value:2.83E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.25|P-value:1.56E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.03|P-value:3.87E-4 ID:SGTC_2085|Compound:5307070|FD-Score:-3.81|P-value:1.07E-5 ID:SGTC_2086|Compound:5274770|FD-Score:-3.10|P-value:2.71E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.94|P-value:5.38E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.00|P-value:4.04E-4 ID:SGTC_2423|Compound:st077119|FD-Score:-3.45|P-value:5.77E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.00|P-value:3.98E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.37|P-value:5.93E-7 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.33|P-value:1.07E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.64|P-value:2.38E-5 ID:SGTC_2955|Compound:9076254|FD-Score:4.43|P-value:4.32E-7 ID:SGTC_3013|Compound:9080133|FD-Score:2.92|P-value:5.83E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.37|P-value:8.63E-5 ID:SGTC_3318|Compound:9139014|FD-Score:-3.03|P-value:3.66E-4

Top fitness defect scores for YDL146W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_149 3970-0822 6.29 46.1 µM 1.35E-12 tubulin folding & SWR complex 32 1.63E-10
2 SGTC_390 dec-rvkr-cmk 4.88 16.7 µM 2.81E-8 calcium & mitochondrial duress 12 5.21E-7
3 SGTC_739 1486-1183 4.64 203.0 µM 1.21E-7 10 1.71E-6
4 SGTC_2955 9076254 4.43 12.2 µM 4.32E-7 10 4.81E-6
5 SGTC_2584 androsterone 4.37 48.7 µM 5.93E-7 cell wall 100 6.23E-6
6 SGTC_212 3807-4682 4.35 38.8 µM 6.45E-7 tubulin folding & SWR complex 48 6.67E-6
7 SGTC_1100 amiodarone 3.88 5.1 µM 7.91E-6 16 5.14E-5
8 SGTC_1800 st050079 3.61 29.1 µM 3.02E-5 NEO1-PIK1 149 1.54E-4
9 SGTC_1784 st039334 3.38 36.3 µM 8.74E-5 33 3.66E-4
10 SGTC_1607 st000305 3.34 50.6 µM 1.01E-4 33 4.12E-4
11 SGTC_2741 miltefosine 3.33 3.0 µM 1.07E-4 53 4.34E-4
12 SGTC_1945 st075991 3.25 56.6 µM 1.56E-4 60S ribosome export 45 5.87E-4
13 SGTC_1434 4073-0095 3.15 163.0 µM 2.37E-4 34 8.30E-4
14 SGTC_1217 1183-1422 3.07 255.0 µM 3.26E-4 41 0.00108
15 SGTC_1597 7-benzyloxygramine 3.06 47.5 µM 3.37E-4 calcium & mitochondrial duress 215 0.00111
16 SGTC_1043 0916-0009 3.05 19.3 µM 3.44E-4 65 0.00113
17 SGTC_150 3970-0969 3.03 77.2 µM 3.80E-4 121 0.00122
18 SGTC_2009 4012297 3.03 12.1 µM 3.87E-4 cell wall signaling 110 0.00124
19 SGTC_2103 5378138 2.94 10.0 µM 5.38E-4 65 0.00162
20 SGTC_3013 9080133 2.92 71.4 µM 5.83E-4 68 0.00173

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.236 1.26E-43 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.147 1.09E-17 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
0.147 1.31E-17 YOR094W ARF3 hom Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity
0.144 5.38E-17 YMR138W CIN4 hom GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
0.137 1.47E-15 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.131 2.59E-14 YML035C AMD1 hom AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
0.128 1.13E-13 YNR040W_p YNR040W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.124 6.38E-13 YBR216C YBP1 hom Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
0.121 1.86E-12 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.115 2.26E-11 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.114 3.08E-11 YBL001C ECM15 hom Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
0.112 7.53E-11 YML094W GIM5 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.112 8.88E-11 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.112 9.52E-11 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.107 4.77E-10 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress