YDL149W / ATG9

Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL149W / ATG9

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:3.86|P-value:7.38E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-2.87|P-value:5.70E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:2.94|P-value:4.80E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.63|P-value:2.34E-5 ID:SGTC_316|Compound:k060-0003|FD-Score:2.90|P-value:5.80E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.84|P-value:7.16E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.07|P-value:2.50E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.60|P-value:2.60E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:2.94|P-value:4.90E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.23|P-value:1.26E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.01|P-value:3.70E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.28|P-value:1.18E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-2.84|P-value:6.27E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.03|P-value:3.35E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.48|P-value:3.97E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.17|P-value:1.65E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-4.55|P-value:1.30E-7 ID:SGTC_2257|Compound:7960413|FD-Score:3.38|P-value:7.55E-5 ID:SGTC_2585|Compound:alliin|FD-Score:3.13|P-value:2.26E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.23|P-value:1.45E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:3.76|P-value:1.20E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.08|P-value:2.30E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.01|P-value:3.59E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.32|P-value:8.26E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:3.86|P-value:7.38E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:-2.87|P-value:5.70E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:2.94|P-value:4.80E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.63|P-value:2.34E-5 ID:SGTC_316|Compound:k060-0003|FD-Score:2.90|P-value:5.80E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:2.84|P-value:7.16E-4 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.07|P-value:2.50E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.60|P-value:2.60E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:2.94|P-value:4.90E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.23|P-value:1.26E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.01|P-value:3.70E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.28|P-value:1.18E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-2.84|P-value:6.27E-4 ID:SGTC_1919|Compound:st067113|FD-Score:3.03|P-value:3.35E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-3.48|P-value:3.97E-5 ID:SGTC_2058|Compound:5236698|FD-Score:-3.17|P-value:1.65E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-4.55|P-value:1.30E-7 ID:SGTC_2257|Compound:7960413|FD-Score:3.38|P-value:7.55E-5 ID:SGTC_2585|Compound:alliin|FD-Score:3.13|P-value:2.26E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.23|P-value:1.45E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:3.76|P-value:1.20E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.08|P-value:2.30E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.01|P-value:3.59E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.32|P-value:8.26E-5

Top fitness defect scores for YDL149W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2780 5215298 4.08 71.4 µM 2.30E-6 31 2.27E-5
2 SGTC_127 0180-0333 3.86 59.1 µM 7.38E-6 mitochondrial response to ROS 50 5.75E-5
3 SGTC_2647 atorvastatin 3.76 60.2 µM 1.20E-5 azole & statin 20 8.45E-5
4 SGTC_311 1315-0136 3.63 58.0 µM 2.34E-5 14 1.44E-4
5 SGTC_875 0709-0210 3.60 25.7 µM 2.60E-5 fatty acid desaturase (OLE1) 41 1.57E-4
6 SGTC_2257 7960413 3.38 200.0 µM 7.55E-5 NEO1-PIK1 82 3.68E-4
7 SGTC_1680 diphenhydramine 3.28 100.0 µM 1.18E-4 NEO1 61 5.26E-4
8 SGTC_2602 kawain 3.23 100.0 µM 1.45E-4 57 6.21E-4
9 SGTC_2585 alliin 3.13 100.0 µM 2.26E-4 68 8.87E-4
10 SGTC_1919 st067113 3.03 52.3 µM 3.35E-4 TSC3-RPN4 77 0.00122
11 SGTC_2805 7950783 3.01 58.4 µM 3.59E-4 69 0.00128
12 SGTC_1405 3909-7894 3.01 12.0 µM 3.70E-4 60S ribosome export 41 0.00132
13 SGTC_159 3970-0959 2.94 50.0 µM 4.80E-4 174 0.00162
14 SGTC_1078 idebenone 2.94 36.1 µM 4.90E-4 Golgi 103 0.00165
15 SGTC_316 k060-0003 2.90 58.6 µM 5.80E-4 47 0.00189
16 SGTC_563 tetrahydrosecaminediol 2.84 614.0 µM 7.16E-4 amide catabolism 73 0.00224
17 SGTC_551 k048-0028 2.69 34.9 µM 0.00129 87 0.00360
18 SGTC_330 1488-0319 2.68 80.1 µM 0.00132 RPP1 & pyrimidine depletion 78 0.00367
19 SGTC_1432 4049-0298 2.67 68.4 µM 0.00140 60 0.00384
20 SGTC_359 0081-0058 2.65 15.9 µM 0.00146 60S ribosome export 135 0.00398

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.168 1.23E-22 YHL040C ARN1 hom ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
0.142 1.65E-16 YHR106W TRR2 hom Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
0.103 2.11E-9 YML116W-A_d YML116W-A_d hom Putative protein of unknown function
0.102 3.70E-9 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.102 3.76E-9 YGR197C SNG1 hom Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.101 3.86E-9 YER115C SPR6 hom Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
0.100 6.84E-9 YPL109C_p YPL109C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.099 1.07E-8 YMR074C YMR074C hom Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
0.097 1.55E-8 YPR155C NCA2 hom Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
0.096 2.19E-8 YER067W RGI1 hom Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
0.095 4.12E-8 YCR073C SSK22 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p
0.090 1.75E-7 YBL057C PTH2 hom One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
0.089 2.60E-7 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
0.087 4.17E-7 YBR050C REG2 hom Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease
0.087 4.62E-7 YKL206C ADD66 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly