YDL161W / ENT1

Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL161W / ENT1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.88|P-value:7.29E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:-2.84|P-value:7.51E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.21|P-value:1.24E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:2.81|P-value:8.51E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.78|P-value:1.20E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.33|P-value:1.01E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-3.30|P-value:1.17E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.20|P-value:1.79E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.28|P-value:1.25E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.97|P-value:4.47E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-2.86|P-value:6.93E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:-2.80|P-value:8.95E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.14|P-value:2.22E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.98|P-value:4.36E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:2.82|P-value:8.13E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.63|P-value:2.57E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:2.79|P-value:8.97E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.16|P-value:4.03E-9 ID:SGTC_1015|Compound:4130-4525|FD-Score:2.91|P-value:5.63E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.07|P-value:3.00E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.36|P-value:8.47E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.09|P-value:2.74E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:2.84|P-value:7.51E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.17|P-value:1.97E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-2.79|P-value:9.13E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.97|P-value:4.56E-6 ID:SGTC_1777|Compound:st045864|FD-Score:-2.95|P-value:4.90E-4 ID:SGTC_1798|Compound:st045719|FD-Score:4.63|P-value:1.18E-7 ID:SGTC_1854|Compound:st056233|FD-Score:3.01|P-value:3.82E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.29|P-value:1.20E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.18|P-value:1.51E-6 ID:SGTC_1949|Compound:st076024|FD-Score:-2.85|P-value:7.17E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.41|P-value:7.04E-5 ID:SGTC_2237|Compound:6667747|FD-Score:3.53|P-value:4.05E-5 ID:SGTC_3245|Compound:9134821|FD-Score:2.91|P-value:5.77E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.88|P-value:7.29E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:-2.84|P-value:7.51E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.21|P-value:1.24E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:2.81|P-value:8.51E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.78|P-value:1.20E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.33|P-value:1.01E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-3.30|P-value:1.17E-4 ID:SGTC_303|Compound:1348-1668|FD-Score:-3.20|P-value:1.79E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.28|P-value:1.25E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:2.97|P-value:4.47E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-2.86|P-value:6.93E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:-2.80|P-value:8.95E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.14|P-value:2.22E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:2.98|P-value:4.36E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:2.82|P-value:8.13E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.63|P-value:2.57E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:2.79|P-value:8.97E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.16|P-value:4.03E-9 ID:SGTC_1015|Compound:4130-4525|FD-Score:2.91|P-value:5.63E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.07|P-value:3.00E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:3.36|P-value:8.47E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.09|P-value:2.74E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:2.84|P-value:7.51E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.17|P-value:1.97E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-2.79|P-value:9.13E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.97|P-value:4.56E-6 ID:SGTC_1777|Compound:st045864|FD-Score:-2.95|P-value:4.90E-4 ID:SGTC_1798|Compound:st045719|FD-Score:4.63|P-value:1.18E-7 ID:SGTC_1854|Compound:st056233|FD-Score:3.01|P-value:3.82E-4 ID:SGTC_1913|Compound:st060213|FD-Score:3.29|P-value:1.20E-4 ID:SGTC_1948|Compound:st076329|FD-Score:4.18|P-value:1.51E-6 ID:SGTC_1949|Compound:st076024|FD-Score:-2.85|P-value:7.17E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.41|P-value:7.04E-5 ID:SGTC_2237|Compound:6667747|FD-Score:3.53|P-value:4.05E-5 ID:SGTC_3245|Compound:9134821|FD-Score:2.91|P-value:5.77E-4

Top fitness defect scores for YDL161W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 5.16 1.8 µM 4.03E-9 49 1.23E-7
2 SGTC_1798 st045719 4.63 61.0 µM 1.18E-7 6 1.87E-6
3 SGTC_145 3737-0113 4.21 38.4 µM 1.24E-6 DNA intercalators 67 1.25E-5
4 SGTC_1948 st076329 4.18 66.6 µM 1.51E-6 21 1.47E-5
5 SGTC_65 0798-0905 3.88 35.0 µM 7.29E-6 calcium & mitochondrial duress 88 5.24E-5
6 SGTC_214 4373-3496 3.78 70.0 µM 1.20E-5 66 7.83E-5
7 SGTC_2237 6667747 3.53 47.2 µM 4.05E-5 22 2.10E-4
8 SGTC_1413 3910-0535 3.36 34.4 µM 8.47E-5 27 3.83E-4
9 SGTC_264 promethazine 3.33 12.5 mM 1.01E-4 DNA intercalators 91 4.40E-4
10 SGTC_1913 st060213 3.29 96.1 µM 1.20E-4 63 5.08E-4
11 SGTC_1475 4092-1048 3.17 11.7 µM 1.97E-4 plasma membrane duress 74 7.61E-4
12 SGTC_890 0929-0006 3.14 47.2 µM 2.22E-4 41 8.37E-4
13 SGTC_1416 3966-0321 3.09 25.3 µM 2.74E-4 59 9.92E-4
14 SGTC_1194 1488-0194 3.07 225.0 µM 3.00E-4 72 0.00107
15 SGTC_1854 st056233 3.01 32.9 µM 3.82E-4 amide catabolism 52 0.00130
16 SGTC_901 1112-0062 2.98 60.1 µM 4.36E-4 33 0.00145
17 SGTC_651 3253-0966 2.97 34.4 µM 4.47E-4 calcium & mitochondrial duress 78 0.00148
18 SGTC_1015 4130-4525 2.91 82.0 µM 5.63E-4 60S ribosome export 75 0.00178
19 SGTC_3245 9134821 2.91 49.5 µM 5.77E-4 68 0.00182
20 SGTC_1468 k284-1086 2.84 134.0 µM 7.51E-4 DNA intercalators 121 0.00226

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.283 6.42E-63 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.225 6.88E-40 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.218 2.28E-37 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.213 8.11E-36 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.210 1.26E-34 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.206 2.63E-33 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.204 1.06E-32 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.196 2.28E-30 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.186 1.69E-27 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.184 6.82E-27 YBR067C TIP1 hom Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
0.184 8.20E-27 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.179 1.08E-25 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.178 3.04E-25 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.173 5.83E-24 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.173 6.85E-24 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data