YDL171C / GLT1

NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL171C / GLT1

Click on Significant Values for Screen Details ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.99|P-value:2.69E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.19|P-value:6.27E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-5.27|P-value:4.57E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.72|P-value:8.58E-5 ID:SGTC_938|Compound:3126-1755|FD-Score:3.02|P-value:9.19E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:6.10|P-value:1.96E-10 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.19|P-value:4.98E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.13|P-value:1.07E-5 ID:SGTC_1746|Compound:st038117|FD-Score:6.15|P-value:1.43E-10 ID:SGTC_1786|Compound:berberine|FD-Score:-3.08|P-value:9.44E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.69|P-value:7.16E-5 ID:SGTC_1946|Compound:st076057|FD-Score:3.35|P-value:2.74E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-5.03|P-value:1.75E-7 ID:SGTC_1996|Compound:st072960|FD-Score:3.78|P-value:4.92E-5 ID:SGTC_1997|Compound:st073024|FD-Score:3.10|P-value:6.83E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.41|P-value:2.18E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.55|P-value:1.28E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.45|P-value:2.37E-6 ID:SGTC_2359|Compound:9039622|FD-Score:3.92|P-value:2.78E-5 ID:SGTC_2430|Compound:5571206|FD-Score:3.24|P-value:4.24E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-3.07|P-value:9.72E-4 ID:SGTC_2693|Compound:st077581|FD-Score:5.23|P-value:4.03E-8 ID:SGTC_2794|Compound:7785382|FD-Score:4.51|P-value:1.82E-6 ID:SGTC_2850|Compound:9015429|FD-Score:-5.91|P-value:1.01E-9 ID:SGTC_2859|Compound:9028643|FD-Score:5.26|P-value:3.27E-8 ID:SGTC_2886|Compound:9058944|FD-Score:3.69|P-value:7.35E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.70|P-value:6.93E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.95|P-value:2.39E-5 ID:SGTC_2956|Compound:9076196|FD-Score:-3.66|P-value:1.07E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-5.98|P-value:6.63E-10 ID:SGTC_2962|Compound:9082311|FD-Score:-3.15|P-value:7.41E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.53|P-value:1.81E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.44|P-value:1.96E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.17|P-value:6.82E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.40|P-value:2.29E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.99|P-value:2.69E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.19|P-value:6.27E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-5.27|P-value:4.57E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.72|P-value:8.58E-5 ID:SGTC_938|Compound:3126-1755|FD-Score:3.02|P-value:9.19E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:6.10|P-value:1.96E-10 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.19|P-value:4.98E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.13|P-value:1.07E-5 ID:SGTC_1746|Compound:st038117|FD-Score:6.15|P-value:1.43E-10 ID:SGTC_1786|Compound:berberine|FD-Score:-3.08|P-value:9.44E-4 ID:SGTC_1924|Compound:st058459|FD-Score:3.69|P-value:7.16E-5 ID:SGTC_1946|Compound:st076057|FD-Score:3.35|P-value:2.74E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-5.03|P-value:1.75E-7 ID:SGTC_1996|Compound:st072960|FD-Score:3.78|P-value:4.92E-5 ID:SGTC_1997|Compound:st073024|FD-Score:3.10|P-value:6.83E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.41|P-value:2.18E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.55|P-value:1.28E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.45|P-value:2.37E-6 ID:SGTC_2359|Compound:9039622|FD-Score:3.92|P-value:2.78E-5 ID:SGTC_2430|Compound:5571206|FD-Score:3.24|P-value:4.24E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-3.07|P-value:9.72E-4 ID:SGTC_2693|Compound:st077581|FD-Score:5.23|P-value:4.03E-8 ID:SGTC_2794|Compound:7785382|FD-Score:4.51|P-value:1.82E-6 ID:SGTC_2850|Compound:9015429|FD-Score:-5.91|P-value:1.01E-9 ID:SGTC_2859|Compound:9028643|FD-Score:5.26|P-value:3.27E-8 ID:SGTC_2886|Compound:9058944|FD-Score:3.69|P-value:7.35E-5 ID:SGTC_2932|Compound:9016711|FD-Score:3.70|P-value:6.93E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.95|P-value:2.39E-5 ID:SGTC_2956|Compound:9076196|FD-Score:-3.66|P-value:1.07E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-5.98|P-value:6.63E-10 ID:SGTC_2962|Compound:9082311|FD-Score:-3.15|P-value:7.41E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.53|P-value:1.81E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.44|P-value:1.96E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.17|P-value:6.82E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.40|P-value:2.29E-4

Top fitness defect scores for YDL171C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1746 st038117 6.15 42.0 µM 1.43E-10 5 3.95E-10
2 SGTC_996 3970-0799 6.10 1.8 µM 1.96E-10 32 5.34E-10
3 SGTC_2859 9028643 5.26 45.5 µM 3.27E-8 4 7.12E-8
4 SGTC_2693 st077581 5.23 40.7 µM 4.03E-8 10 8.70E-8
5 SGTC_2794 7785382 4.51 27.3 µM 1.82E-6 18 3.32E-6
6 SGTC_2316 5844804 4.45 197.4 µM 2.37E-6 16 4.26E-6
7 SGTC_1739 st037196 4.13 92.5 µM 1.07E-5 17 1.80E-5
8 SGTC_2934 9036370 3.95 36.9 µM 2.39E-5 33 3.88E-5
9 SGTC_2359 9039622 3.92 200.0 µM 2.78E-5 endomembrane recycling 25 4.49E-5
10 SGTC_1996 st072960 3.78 66.8 µM 4.92E-5 16 7.73E-5
11 SGTC_2932 9016711 3.70 44.5 µM 6.93E-5 54 1.07E-4
12 SGTC_1924 st058459 3.69 75.1 µM 7.16E-5 16 1.11E-4
13 SGTC_2886 9058944 3.69 52.0 µM 7.35E-5 2 1.13E-4
14 SGTC_2121 5257820 3.55 149.6 µM 1.28E-4 16 1.93E-4
15 SGTC_3018 9082602 3.44 49.5 µM 1.96E-4 63 2.90E-4
16 SGTC_2002 4023503 3.41 131.0 µM 2.18E-4 mitochondrial processes 60 3.21E-4
17 SGTC_3354 9151612 3.40 71.4 µM 2.29E-4 32 3.35E-4
18 SGTC_1946 st076057 3.35 65.1 µM 2.74E-4 16 3.98E-4
19 SGTC_2430 5571206 3.24 200.0 µM 4.24E-4 89 6.04E-4
20 SGTC_1270 0831-1170 3.19 17.3 µM 4.98E-4 32 7.04E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.268 2.71E-56 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.249 1.74E-48 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.245 6.70E-47 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.195 5.17E-30 YGL083W SCY1 hom Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase
0.193 2.46E-29 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.185 2.60E-27 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.184 4.78E-27 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.179 1.11E-25 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.176 1.07E-24 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.175 1.35E-24 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.174 2.34E-24 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.174 2.97E-24 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.169 7.31E-23 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.168 9.20E-23 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
0.168 1.11E-22 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress