YDL175C / AIR2

RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL175C / AIR2

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:6.28|P-value:7.03E-10 ID:SGTC_14|Compound:0354-0009|FD-Score:4.67|P-value:3.36E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:3.22|P-value:9.72E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.27|P-value:1.95E-5 ID:SGTC_698|Compound:k064-0052|FD-Score:-3.25|P-value:8.50E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.25|P-value:2.16E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.35|P-value:6.21E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.00|P-value:7.15E-7 ID:SGTC_1070|Compound:indatraline|FD-Score:-4.16|P-value:3.01E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:-3.21|P-value:9.73E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.04|P-value:5.72E-7 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.22|P-value:9.70E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.34|P-value:1.44E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.90|P-value:6.41E-9 ID:SGTC_1678|Compound:st019265|FD-Score:-4.07|P-value:4.38E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.23|P-value:9.32E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.53|P-value:3.32E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.31|P-value:7.20E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.24|P-value:2.20E-5 ID:SGTC_2252|Compound:omeprazole|FD-Score:5.76|P-value:1.40E-8 ID:SGTC_2259|Compound:7964009|FD-Score:-3.83|P-value:1.09E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-4.33|P-value:1.50E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.36|P-value:5.96E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-4.17|P-value:2.83E-5 ID:SGTC_2769|Compound:toremifene|FD-Score:-4.04|P-value:4.88E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.27|P-value:8.23E-4 ID:SGTC_2803|Compound:7707263|FD-Score:3.53|P-value:3.35E-4 ID:SGTC_2830|Compound:7992007|FD-Score:5.34|P-value:1.34E-7 ID:SGTC_2833|Compound:9001315|FD-Score:-6.20|P-value:1.15E-9 ID:SGTC_3075|Compound:9116269|FD-Score:-4.27|P-value:1.88E-5 ID:SGTC_3106|Compound:9121286|FD-Score:3.22|P-value:9.78E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.26|P-value:8.22E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-4.00|P-value:5.65E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.68|P-value:1.99E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.59|P-value:2.74E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:6.28|P-value:7.03E-10 ID:SGTC_14|Compound:0354-0009|FD-Score:4.67|P-value:3.36E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:3.22|P-value:9.72E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.27|P-value:1.95E-5 ID:SGTC_698|Compound:k064-0052|FD-Score:-3.25|P-value:8.50E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.25|P-value:2.16E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.35|P-value:6.21E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.00|P-value:7.15E-7 ID:SGTC_1070|Compound:indatraline|FD-Score:-4.16|P-value:3.01E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:-3.21|P-value:9.73E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-5.04|P-value:5.72E-7 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.22|P-value:9.70E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:4.34|P-value:1.44E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.90|P-value:6.41E-9 ID:SGTC_1678|Compound:st019265|FD-Score:-4.07|P-value:4.38E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.23|P-value:9.32E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.53|P-value:3.32E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.31|P-value:7.20E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.24|P-value:2.20E-5 ID:SGTC_2252|Compound:omeprazole|FD-Score:5.76|P-value:1.40E-8 ID:SGTC_2259|Compound:7964009|FD-Score:-3.83|P-value:1.09E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-4.33|P-value:1.50E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.36|P-value:5.96E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-4.17|P-value:2.83E-5 ID:SGTC_2769|Compound:toremifene|FD-Score:-4.04|P-value:4.88E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.27|P-value:8.23E-4 ID:SGTC_2803|Compound:7707263|FD-Score:3.53|P-value:3.35E-4 ID:SGTC_2830|Compound:7992007|FD-Score:5.34|P-value:1.34E-7 ID:SGTC_2833|Compound:9001315|FD-Score:-6.20|P-value:1.15E-9 ID:SGTC_3075|Compound:9116269|FD-Score:-4.27|P-value:1.88E-5 ID:SGTC_3106|Compound:9121286|FD-Score:3.22|P-value:9.78E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.26|P-value:8.22E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-4.00|P-value:5.65E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.68|P-value:1.99E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.59|P-value:2.74E-4

Top fitness defect scores for YDL175C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_8 0354-0001 6.28 70.0 µM 7.03E-10 25 1.64E-10
2 SGTC_1597 7-benzyloxygramine 5.90 47.5 µM 6.41E-9 calcium & mitochondrial duress 52 1.77E-9
3 SGTC_2252 omeprazole 5.76 400.0 µM 1.40E-8 1 4.11E-9
4 SGTC_2830 7992007 5.34 71.4 µM 1.34E-7 7 4.63E-8
5 SGTC_14 0354-0009 4.67 295.0 µM 3.36E-6 16 1.48E-6
6 SGTC_1577 3(2'-chlorophenyl)-7-ethoxycoumarin 4.34 66.5 µM 1.44E-5 13 7.02E-6
7 SGTC_159 3970-0959 4.27 50.0 µM 1.95E-5 64 9.77E-6
8 SGTC_753 1322-0200 4.25 289.0 µM 2.16E-5 11 1.09E-5
9 SGTC_3204 9114183 3.68 49.5 µM 1.99E-4 40 1.18E-4
10 SGTC_3272 9137413 3.59 49.5 µM 2.74E-4 21 1.66E-4
11 SGTC_2009 4012297 3.57 12.1 µM 2.89E-4 cell wall signaling 80 1.76E-4
12 SGTC_1821 st053465 3.53 65.6 µM 3.32E-4 15 2.04E-4
13 SGTC_2803 7707263 3.53 71.4 µM 3.35E-4 42 2.06E-4
14 SGTC_911 3456-4043 3.35 236.0 µM 6.21E-4 mitochondrial processes 50 4.00E-4
15 SGTC_1836 st055642 3.31 81.0 µM 7.20E-4 44 4.69E-4
16 SGTC_2777 climbazole 3.27 1.5 µM 8.23E-4 iron homeostasis 80 5.41E-4
17 SGTC_1820 st053765 3.23 18.6 µM 9.32E-4 RPP1 & pyrimidine depletion 47 6.18E-4
18 SGTC_1495 4469-0029 3.22 237.0 µM 9.70E-4 163 6.45E-4
19 SGTC_91 0417-1751 3.22 58.4 µM 9.72E-4 Golgi 87 6.46E-4
20 SGTC_3106 9121286 3.22 49.5 µM 9.78E-4 40 6.51E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 1.30E-23 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.122 1.36E-12 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.120 2.60E-12 YDL236W PHO13 hom Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity
0.115 1.99E-11 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.112 9.20E-11 YOL075C_p YOL075C_p hom Putative ABC transporter
0.108 3.20E-10 YLR404W FLD1 hom Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
0.103 2.43E-9 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.101 4.02E-9 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.098 1.22E-8 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.096 2.18E-8 YFR051C RET2 het Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.096 2.35E-8 YOR102W_d YOR102W_d het Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
0.091 1.11E-7 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.091 1.32E-7 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.090 2.09E-7 YDR276C PMP3 hom Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
0.088 2.94E-7 YHL029C_p OCA5_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts