YDL197C / ASF2

Anti-silencing protein that causes derepression of silent loci when overexpressed

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL197C / ASF2

Click on Significant Values for Screen Details ID:SGTC_319|Compound:1348-1596|FD-Score:3.37|P-value:7.10E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-2.74|P-value:9.13E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:2.83|P-value:6.97E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.47|P-value:4.62E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:-4.04|P-value:2.21E-6 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.95|P-value:4.47E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:-2.73|P-value:9.44E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:2.96|P-value:4.28E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.62|P-value:9.08E-8 ID:SGTC_1370|Compound:2001-0092|FD-Score:7.05|P-value:7.88E-16 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.76|P-value:8.25E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-2.76|P-value:8.36E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.79|P-value:2.54E-11 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.04|P-value:3.61E-12 ID:SGTC_1705|Compound:st027868|FD-Score:-3.57|P-value:2.52E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.05|P-value:2.63E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.25|P-value:1.10E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.65|P-value:1.64E-5 ID:SGTC_2037|Compound:5169046|FD-Score:4.71|P-value:5.27E-8 ID:SGTC_2048|Compound:5246248|FD-Score:2.88|P-value:5.92E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.39|P-value:6.51E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-3.16|P-value:1.66E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:2.93|P-value:4.78E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.39|P-value:5.92E-5 ID:SGTC_2824|Compound:7998404|FD-Score:2.99|P-value:3.69E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-2.79|P-value:7.35E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.14|P-value:2.00E-4 ID:SGTC_2898|Compound:9056270|FD-Score:2.91|P-value:5.17E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-2.76|P-value:8.47E-4 ID:SGTC_3038|Compound:9091290|FD-Score:2.98|P-value:3.97E-4 ID:SGTC_3042|Compound:9091578|FD-Score:2.97|P-value:4.02E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-2.80|P-value:7.06E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-3.60|P-value:2.17E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.04|P-value:2.98E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-4.98|P-value:7.86E-9 ID:SGTC_3217|Compound:9129673|FD-Score:4.64|P-value:7.95E-8 ID:SGTC_3235|Compound:9132602|FD-Score:-2.84|P-value:6.03E-4 ID:SGTC_3264|Compound:9137442|FD-Score:2.86|P-value:6.41E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.37|P-value:7.10E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-2.74|P-value:9.13E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:2.83|P-value:6.97E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:3.47|P-value:4.62E-5 ID:SGTC_1037|Compound:k284-1091|FD-Score:-4.04|P-value:2.21E-6 ID:SGTC_1262|Compound:0737-0114|FD-Score:2.95|P-value:4.47E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:-2.73|P-value:9.44E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:2.96|P-value:4.28E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:4.62|P-value:9.08E-8 ID:SGTC_1370|Compound:2001-0092|FD-Score:7.05|P-value:7.88E-16 ID:SGTC_1372|Compound:2752-0194|FD-Score:-2.76|P-value:8.25E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-2.76|P-value:8.36E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.79|P-value:2.54E-11 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.04|P-value:3.61E-12 ID:SGTC_1705|Compound:st027868|FD-Score:-3.57|P-value:2.52E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.05|P-value:2.63E-4 ID:SGTC_1750|Compound:st081859|FD-Score:-3.25|P-value:1.10E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.65|P-value:1.64E-5 ID:SGTC_2037|Compound:5169046|FD-Score:4.71|P-value:5.27E-8 ID:SGTC_2048|Compound:5246248|FD-Score:2.88|P-value:5.92E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.39|P-value:6.51E-5 ID:SGTC_2400|Compound:5203494|FD-Score:-3.16|P-value:1.66E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:2.93|P-value:4.78E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.39|P-value:5.92E-5 ID:SGTC_2824|Compound:7998404|FD-Score:2.99|P-value:3.69E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-2.79|P-value:7.35E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.14|P-value:2.00E-4 ID:SGTC_2898|Compound:9056270|FD-Score:2.91|P-value:5.17E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-2.76|P-value:8.47E-4 ID:SGTC_3038|Compound:9091290|FD-Score:2.98|P-value:3.97E-4 ID:SGTC_3042|Compound:9091578|FD-Score:2.97|P-value:4.02E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-2.80|P-value:7.06E-4 ID:SGTC_3179|Compound:9107801|FD-Score:-3.60|P-value:2.17E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.04|P-value:2.98E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-4.98|P-value:7.86E-9 ID:SGTC_3217|Compound:9129673|FD-Score:4.64|P-value:7.95E-8 ID:SGTC_3235|Compound:9132602|FD-Score:-2.84|P-value:6.03E-4 ID:SGTC_3264|Compound:9137442|FD-Score:2.86|P-value:6.41E-4

Top fitness defect scores for YDL197C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1370 2001-0092 7.05 44.6 µM 7.88E-16 3 8.91E-13
2 SGTC_2037 5169046 4.71 200.0 µM 5.27E-8 60S ribosome export 5 1.25E-6
3 SGTC_3217 9129673 4.64 49.5 µM 7.95E-8 17 1.73E-6
4 SGTC_1369 1866-0067 4.62 208.0 µM 9.08E-8 RPP1 & pyrimidine depletion 22 1.92E-6
5 SGTC_940 3232-1287 3.47 54.8 µM 4.62E-5 NEO1 57 2.65E-4
6 SGTC_2100 5377183 3.39 190.0 µM 6.51E-5 60S ribosome export 23 3.48E-4
7 SGTC_319 1348-1596 3.37 45.5 µM 7.10E-5 RSC & ERG11 33 3.72E-4
8 SGTC_2877 9040724 3.14 71.4 µM 2.00E-4 43 8.48E-4
9 SGTC_3197 9113331 3.04 49.5 µM 2.98E-4 86 0.00117
10 SGTC_2824 7998404 2.99 52.0 µM 3.69E-4 69 0.00138
11 SGTC_3038 9091290 2.98 49.5 µM 3.97E-4 61 0.00146
12 SGTC_3042 9091578 2.97 49.5 µM 4.02E-4 ERG2 28 0.00148
13 SGTC_1312 1218-2183 2.96 165.0 µM 4.28E-4 36 0.00155
14 SGTC_1262 0737-0114 2.95 49.6 µM 4.47E-4 69 0.00161
15 SGTC_2516 gossypol 2.93 71.5 µM 4.78E-4 63 0.00170
16 SGTC_2898 9056270 2.91 58.4 µM 5.17E-4 29 0.00181
17 SGTC_2048 5246248 2.88 200.0 µM 5.92E-4 35 0.00201
18 SGTC_3264 9137442 2.86 49.5 µM 6.41E-4 40 0.00214
19 SGTC_936 3005-4620 2.83 401.0 µM 6.97E-4 92 0.00229
20 SGTC_2876 9059903 2.73 26.0 µM 0.00104 68 0.00315

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.310 1.57E-75 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.304 9.19E-73 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.293 2.09E-67 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.286 8.65E-64 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.280 1.53E-61 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.263 4.22E-54 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.260 7.40E-53 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.257 1.51E-51 YLR213C CRR1 hom Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
0.248 4.81E-48 YFR042W KEG1 het Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
0.240 3.16E-45 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.240 2.97E-45 YLR136C TIS11 hom mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.239 8.71E-45 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.237 4.31E-44 YKL166C TPK3 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
0.236 1.01E-43 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.235 1.65E-43 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress