YDR014W / RAD61

Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR014W / RAD61

Click on Significant Values for Screen Details ID:SGTC_96|Compound:0929-0093|FD-Score:3.43|P-value:8.59E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.82|P-value:4.98E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.58|P-value:5.17E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.95|P-value:2.47E-8 ID:SGTC_579|Compound:4491-1009|FD-Score:-3.46|P-value:7.35E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.10|P-value:8.67E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.64|P-value:4.27E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.57|P-value:5.19E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.99|P-value:1.29E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.48|P-value:7.15E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.54|P-value:5.95E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:-3.66|P-value:3.83E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.76|P-value:2.84E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.48|P-value:1.94E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.64|P-value:4.35E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.76|P-value:2.87E-4 ID:SGTC_1691|Compound:st019933|FD-Score:-4.36|P-value:3.05E-5 ID:SGTC_1707|Compound:st032323|FD-Score:5.08|P-value:1.63E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:6.08|P-value:1.30E-8 ID:SGTC_1931|Compound:st074438|FD-Score:3.86|P-value:2.05E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.54|P-value:5.90E-4 ID:SGTC_2105|Compound:5350118|FD-Score:4.33|P-value:3.69E-5 ID:SGTC_2110|Compound:5483698|FD-Score:3.64|P-value:4.24E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.75|P-value:2.95E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.38|P-value:9.93E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.90|P-value:3.64E-6 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.54|P-value:5.95E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.74|P-value:3.04E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.44|P-value:2.39E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.92|P-value:1.68E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.94|P-value:1.44E-4 ID:SGTC_2803|Compound:7707263|FD-Score:6.13|P-value:9.59E-9 ID:SGTC_2889|Compound:9059656|FD-Score:3.86|P-value:2.07E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.50|P-value:6.78E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.43|P-value:8.59E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.82|P-value:4.98E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.58|P-value:5.17E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.95|P-value:2.47E-8 ID:SGTC_579|Compound:4491-1009|FD-Score:-3.46|P-value:7.35E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:4.10|P-value:8.67E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.64|P-value:4.27E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.57|P-value:5.19E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.99|P-value:1.29E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.48|P-value:7.15E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.54|P-value:5.95E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:-3.66|P-value:3.83E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:3.76|P-value:2.84E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.48|P-value:1.94E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.64|P-value:4.35E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.76|P-value:2.87E-4 ID:SGTC_1691|Compound:st019933|FD-Score:-4.36|P-value:3.05E-5 ID:SGTC_1707|Compound:st032323|FD-Score:5.08|P-value:1.63E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:6.08|P-value:1.30E-8 ID:SGTC_1931|Compound:st074438|FD-Score:3.86|P-value:2.05E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.54|P-value:5.90E-4 ID:SGTC_2105|Compound:5350118|FD-Score:4.33|P-value:3.69E-5 ID:SGTC_2110|Compound:5483698|FD-Score:3.64|P-value:4.24E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.75|P-value:2.95E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.38|P-value:9.93E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.90|P-value:3.64E-6 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.54|P-value:5.95E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.74|P-value:3.04E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.44|P-value:2.39E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.92|P-value:1.68E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.94|P-value:1.44E-4 ID:SGTC_2803|Compound:7707263|FD-Score:6.13|P-value:9.59E-9 ID:SGTC_2889|Compound:9059656|FD-Score:3.86|P-value:2.07E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.50|P-value:6.78E-4

Top fitness defect scores for YDR014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2803 7707263 6.13 71.4 µM 9.59E-9 5 4.31E-10
2 SGTC_1880 MG-132 6.08 525.0 µM 1.30E-8 3 6.19E-10
3 SGTC_268 camptothecin 5.95 39.6 µM 2.47E-8 DNA damage response 29 1.32E-9
4 SGTC_1707 st032323 5.08 6.3 µM 1.63E-6 DNA damage response 33 1.87E-7
5 SGTC_2520 obtusaquinone 4.90 4.7 µM 3.64E-6 4 4.86E-7
6 SGTC_114 0364-0001 4.82 490.3 µM 4.98E-6 19 7.05E-7
7 SGTC_2689 tacrolimus 4.44 100.0 µM 2.39E-5 34 4.50E-6
8 SGTC_2105 5350118 4.33 200.0 µM 3.69E-5 19 7.51E-6
9 SGTC_583 1488-1091 4.10 88.9 µM 8.67E-5 15 2.06E-5
10 SGTC_1079 idarubicin 3.99 2.4 µM 1.29E-4 DNA damage response 21 3.28E-5
11 SGTC_2697 mebhydrolin 3.92 72.4 µM 1.68E-4 NEO1 63 4.49E-5
12 SGTC_1931 st074438 3.86 57.1 µM 2.05E-4 19 5.67E-5
13 SGTC_2889 9059656 3.86 52.0 µM 2.07E-4 9 5.75E-5
14 SGTC_1210 3013-0140 3.76 750.0 nM 2.84E-4 6 8.34E-5
15 SGTC_1674 st016616 3.76 23.6 µM 2.87E-4 34 8.43E-5
16 SGTC_2148 5546263 3.75 113.6 µM 2.95E-4 27 8.71E-5
17 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 3.74 100.0 µM 3.04E-4 28 9.03E-5
18 SGTC_2110 5483698 3.64 200.0 µM 4.24E-4 TSC3-RPN4 30 1.34E-4
19 SGTC_584 0986-0246 3.64 15.4 µM 4.27E-4 heme requiring 18 1.35E-4
20 SGTC_1464 k208-0027 3.64 110.0 µM 4.35E-4 32 1.38E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 1.00E-25 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.168 1.03E-22 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.167 2.55E-22 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.139 6.89E-16 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.135 4.97E-15 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.130 4.36E-14 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.129 7.21E-14 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.128 9.95E-14 YAL062W GDH3 hom NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
0.126 2.60E-13 YJR025C BNA1 hom 3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
0.122 1.25E-12 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.120 3.12E-12 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.114 3.13E-11 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.114 4.16E-11 YOR215C AIM41 hom Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
0.106 7.26E-10 YPL003W ULA1 hom Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
0.103 2.55E-9 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p