YDR020C_p / DAS2

Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR020C / DAS2

Click on Significant Values for Screen Details ID:SGTC_5|Compound:0352-0601|FD-Score:-3.01|P-value:7.34E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.13|P-value:4.77E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.09|P-value:4.38E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.15|P-value:3.48E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.95|P-value:7.49E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.66|P-value:4.28E-5 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.14|P-value:4.59E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.63|P-value:6.18E-5 ID:SGTC_730|Compound:1436-0052|FD-Score:3.27|P-value:2.22E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.27|P-value:3.08E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.62|P-value:6.47E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:3.04|P-value:5.41E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.17|P-value:3.29E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.07|P-value:4.86E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.17|P-value:3.25E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:2.88|P-value:9.76E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:5.51|P-value:1.78E-9 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.07|P-value:5.91E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.04|P-value:5.44E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:2.91|P-value:8.77E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.99|P-value:6.40E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.12|P-value:3.92E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.01|P-value:1.08E-5 ID:SGTC_1745|Compound:st081495|FD-Score:7.51|P-value:4.58E-16 ID:SGTC_1814|Compound:st052110|FD-Score:-2.93|P-value:9.84E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.47|P-value:9.53E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-4.12|P-value:6.59E-6 ID:SGTC_1978|Compound:st071761|FD-Score:-3.39|P-value:1.72E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-3.53|P-value:9.30E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-3.24|P-value:3.09E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-3.12|P-value:4.83E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.34|P-value:1.67E-4 ID:SGTC_2083|Compound:5306046|FD-Score:4.25|P-value:2.59E-6 ID:SGTC_2084|Compound:5306078|FD-Score:3.52|P-value:7.74E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.31|P-value:2.35E-4 ID:SGTC_2192|Compound:5915882|FD-Score:4.63|P-value:3.40E-7 ID:SGTC_2248|Compound:tofa|FD-Score:2.90|P-value:8.82E-4 ID:SGTC_2320|Compound:7373003|FD-Score:6.22|P-value:1.33E-11 ID:SGTC_2325|Compound:6158266|FD-Score:3.62|P-value:5.11E-5 ID:SGTC_2327|Compound:6189656|FD-Score:4.69|P-value:2.50E-7 ID:SGTC_2402|Compound:5553605|FD-Score:7.27|P-value:3.50E-15 ID:SGTC_2404|Compound:5161160|FD-Score:-4.20|P-value:4.37E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.22|P-value:2.67E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.69|P-value:3.65E-5 ID:SGTC_2676|Compound:harmalol|FD-Score:3.58|P-value:5.88E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.79|P-value:2.38E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.00|P-value:6.21E-4 ID:SGTC_2787|Compound:5284540|FD-Score:2.93|P-value:8.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.70|P-value:3.45E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.57|P-value:6.37E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-4.23|P-value:3.81E-6 ID:SGTC_2812|Compound:7970281|FD-Score:3.85|P-value:1.75E-5 ID:SGTC_2814|Compound:7977482|FD-Score:12.20|P-value:4.22E-39 ID:SGTC_2970|Compound:9090938|FD-Score:3.92|P-value:1.27E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.20|P-value:2.92E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-2.99|P-value:7.87E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.61|P-value:6.83E-5 ID:SGTC_3194|Compound:9111903|FD-Score:3.43|P-value:1.11E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.12|P-value:4.01E-4 ID:SGTC_5|Compound:0352-0601|FD-Score:-3.01|P-value:7.34E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.13|P-value:4.77E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.09|P-value:4.38E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.15|P-value:3.48E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.95|P-value:7.49E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.66|P-value:4.28E-5 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.14|P-value:4.59E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.63|P-value:6.18E-5 ID:SGTC_730|Compound:1436-0052|FD-Score:3.27|P-value:2.22E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.27|P-value:3.08E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.62|P-value:6.47E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:3.04|P-value:5.41E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.17|P-value:3.29E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.07|P-value:4.86E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:3.17|P-value:3.25E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:2.88|P-value:9.76E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:5.51|P-value:1.78E-9 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.07|P-value:5.91E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.04|P-value:5.44E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:2.91|P-value:8.77E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:2.99|P-value:6.40E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.12|P-value:3.92E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.01|P-value:1.08E-5 ID:SGTC_1745|Compound:st081495|FD-Score:7.51|P-value:4.58E-16 ID:SGTC_1814|Compound:st052110|FD-Score:-2.93|P-value:9.84E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.47|P-value:9.53E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-4.12|P-value:6.59E-6 ID:SGTC_1978|Compound:st071761|FD-Score:-3.39|P-value:1.72E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-3.53|P-value:9.30E-5 ID:SGTC_1984|Compound:st072354|FD-Score:-3.24|P-value:3.09E-4 ID:SGTC_1985|Compound:st071382|FD-Score:-3.12|P-value:4.83E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.34|P-value:1.67E-4 ID:SGTC_2083|Compound:5306046|FD-Score:4.25|P-value:2.59E-6 ID:SGTC_2084|Compound:5306078|FD-Score:3.52|P-value:7.74E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.31|P-value:2.35E-4 ID:SGTC_2192|Compound:5915882|FD-Score:4.63|P-value:3.40E-7 ID:SGTC_2248|Compound:tofa|FD-Score:2.90|P-value:8.82E-4 ID:SGTC_2320|Compound:7373003|FD-Score:6.22|P-value:1.33E-11 ID:SGTC_2325|Compound:6158266|FD-Score:3.62|P-value:5.11E-5 ID:SGTC_2327|Compound:6189656|FD-Score:4.69|P-value:2.50E-7 ID:SGTC_2402|Compound:5553605|FD-Score:7.27|P-value:3.50E-15 ID:SGTC_2404|Compound:5161160|FD-Score:-4.20|P-value:4.37E-6 ID:SGTC_2443|Compound:5493264|FD-Score:3.22|P-value:2.67E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.69|P-value:3.65E-5 ID:SGTC_2676|Compound:harmalol|FD-Score:3.58|P-value:5.88E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.79|P-value:2.38E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.00|P-value:6.21E-4 ID:SGTC_2787|Compound:5284540|FD-Score:2.93|P-value:8.15E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.70|P-value:3.45E-5 ID:SGTC_2802|Compound:7925815|FD-Score:3.57|P-value:6.37E-5 ID:SGTC_2804|Compound:7954240|FD-Score:-4.23|P-value:3.81E-6 ID:SGTC_2812|Compound:7970281|FD-Score:3.85|P-value:1.75E-5 ID:SGTC_2814|Compound:7977482|FD-Score:12.20|P-value:4.22E-39 ID:SGTC_2970|Compound:9090938|FD-Score:3.92|P-value:1.27E-5 ID:SGTC_2977|Compound:9011197|FD-Score:3.20|P-value:2.92E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-2.99|P-value:7.87E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.61|P-value:6.83E-5 ID:SGTC_3194|Compound:9111903|FD-Score:3.43|P-value:1.11E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.12|P-value:4.01E-4

Top fitness defect scores for YDR020C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2814 7977482 12.20 43.0 µM 4.22E-39 2 1.72E-34
2 SGTC_1745 st081495 7.51 74.0 µM 4.58E-16 RNA processing & uracil transport 15 2.94E-14
3 SGTC_2402 5553605 7.27 200.0 µM 3.50E-15 1 1.76E-13
4 SGTC_2320 7373003 6.22 200.0 µM 1.33E-11 4 2.45E-10
5 SGTC_1305 1181-0477 5.51 144.0 µM 1.78E-9 6 1.81E-8
6 SGTC_2327 6189656 4.69 200.0 µM 2.50E-7 11 1.39E-6
7 SGTC_2192 5915882 4.63 198.5 µM 3.40E-7 RNA processing & uracil transport 20 1.82E-6
8 SGTC_2083 5306046 4.25 65.9 µM 2.59E-6 20 1.09E-5
9 SGTC_2970 9090938 3.92 41.9 µM 1.27E-5 RNA processing & uracil transport 39 4.40E-5
10 SGTC_2812 7970281 3.85 58.4 µM 1.75E-5 33 5.81E-5
11 SGTC_2708 st078559 3.79 56.0 µM 2.38E-5 7 7.65E-5
12 SGTC_2796 7949634 3.70 45.5 µM 3.45E-5 22 1.06E-4
13 SGTC_2619 obliquin 3.69 87.9 µM 3.65E-5 cell wall 156 1.11E-4
14 SGTC_309 k408-0051 3.66 1.5 µM 4.28E-5 67 1.28E-4
15 SGTC_2325 6158266 3.62 200.0 µM 5.11E-5 36 1.50E-4
16 SGTC_2676 harmalol 3.58 100.0 µM 5.88E-5 mitochondrial processes 20 1.69E-4
17 SGTC_2802 7925815 3.57 54.5 µM 6.37E-5 53 1.82E-4
18 SGTC_2084 5306078 3.52 60.6 µM 7.74E-5 29 2.16E-4
19 SGTC_1836 st055642 3.47 81.0 µM 9.53E-5 35 2.59E-4
20 SGTC_3194 9111903 3.43 49.5 µM 1.11E-4 38 2.96E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.293 2.03E-67 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.188 6.37E-28 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
0.183 1.16E-26 YLR277C YSH1 het Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
0.178 2.70E-25 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.168 1.23E-22 YMR171C EAR1 hom Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
0.152 9.80E-19 YOR060C SLD7 het Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.138 9.80E-16 YBR214W SDS24 hom Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
0.136 3.19E-15 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.134 7.09E-15 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.131 2.74E-14 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.130 3.75E-14 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.125 3.18E-13 YNR012W URK1 hom Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
0.120 3.65E-12 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.118 6.08E-12 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.114 3.66E-11 YIL165C_p YIL165C_p hom Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene