YDR049W / VMS1

Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR049W / VMS1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.76|P-value:3.12E-10 ID:SGTC_73|Compound:0327-0325|FD-Score:3.18|P-value:3.47E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:5.56|P-value:1.23E-9 ID:SGTC_88|Compound:0180-0332|FD-Score:3.17|P-value:3.60E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.62|P-value:5.45E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:3.07|P-value:5.17E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.79|P-value:2.55E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.70|P-value:2.37E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:3.78|P-value:2.60E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:18.10|P-value:7.43E-85 ID:SGTC_168|Compound:3970-0862|FD-Score:4.10|P-value:5.56E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.62|P-value:4.09E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.48|P-value:9.85E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:9.21|P-value:2.06E-23 ID:SGTC_310|Compound:1273-0058|FD-Score:3.32|P-value:1.97E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.87|P-value:8.75E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:3.47|P-value:1.04E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:-2.88|P-value:8.38E-4 ID:SGTC_448|Compound:monastrol|FD-Score:3.57|P-value:6.64E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.25|P-value:9.91E-12 ID:SGTC_514|Compound:4466-0038|FD-Score:7.24|P-value:3.82E-15 ID:SGTC_569|Compound:4130-2812|FD-Score:-3.01|P-value:5.16E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-5.11|P-value:1.47E-8 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.08|P-value:4.01E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:5.23|P-value:1.01E-8 ID:SGTC_676|Compound:falcarinol|FD-Score:3.88|P-value:1.65E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.59|P-value:6.26E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:-3.03|P-value:4.81E-4 ID:SGTC_812|Compound:4251-0442|FD-Score:3.42|P-value:1.29E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.17|P-value:3.51E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.21|P-value:3.00E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.04|P-value:5.81E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.04|P-value:7.64E-6 ID:SGTC_1093|Compound:fluphenazine|FD-Score:2.93|P-value:8.84E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:2.90|P-value:9.86E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.95|P-value:1.16E-5 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.09|P-value:4.79E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.66|P-value:4.60E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.39|P-value:1.14E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.33|P-value:1.89E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-4.23|P-value:2.15E-6 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-5.37|P-value:2.89E-9 ID:SGTC_1720|Compound:st037298|FD-Score:-2.98|P-value:5.80E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.24|P-value:2.13E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:9.62|P-value:2.21E-25 ID:SGTC_2004|Compound:4023728|FD-Score:3.48|P-value:9.78E-5 ID:SGTC_2063|Compound:5246145|FD-Score:-3.05|P-value:4.51E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.35|P-value:1.71E-4 ID:SGTC_2078|Compound:5212016|FD-Score:-3.04|P-value:4.67E-4 ID:SGTC_2080|Compound:5212524|FD-Score:2.90|P-value:9.96E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.29|P-value:2.19E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.26|P-value:2.52E-4 ID:SGTC_2174|Compound:5790901|FD-Score:3.06|P-value:5.47E-4 ID:SGTC_2186|Compound:6124449|FD-Score:5.72|P-value:4.26E-10 ID:SGTC_2190|Compound:6049624|FD-Score:3.23|P-value:2.80E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.98|P-value:1.01E-5 ID:SGTC_2373|Compound:9071805|FD-Score:3.11|P-value:4.40E-4 ID:SGTC_2381|Compound:9035592|FD-Score:4.42|P-value:1.08E-6 ID:SGTC_2384|Compound:9036245|FD-Score:3.36|P-value:1.62E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.38|P-value:1.49E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-5.22|P-value:7.59E-9 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.80|P-value:2.39E-5 ID:SGTC_2570|Compound:licareol|FD-Score:3.35|P-value:1.71E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.49|P-value:7.48E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.53|P-value:7.94E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:8.65|P-value:6.97E-21 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-2.91|P-value:7.51E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:8.38|P-value:1.12E-19 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.13|P-value:4.13E-4 ID:SGTC_2695|Compound:st077686|FD-Score:-3.21|P-value:2.34E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.39|P-value:1.43E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.69|P-value:4.00E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:2.91|P-value:9.49E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.71|P-value:2.76E-5 ID:SGTC_3024|Compound:9090631|FD-Score:2.93|P-value:8.77E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.33|P-value:1.46E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.78|P-value:1.94E-5 ID:SGTC_3044|Compound:9093193|FD-Score:3.19|P-value:3.22E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.53|P-value:6.15E-7 ID:SGTC_3056|Compound:9094900|FD-Score:-2.89|P-value:8.28E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.56|P-value:5.16E-7 ID:SGTC_3153|Compound:9100258|FD-Score:4.09|P-value:6.04E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.08|P-value:4.98E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.76|P-value:3.12E-10 ID:SGTC_73|Compound:0327-0325|FD-Score:3.18|P-value:3.47E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:5.56|P-value:1.23E-9 ID:SGTC_88|Compound:0180-0332|FD-Score:3.17|P-value:3.60E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.62|P-value:5.45E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:3.07|P-value:5.17E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.79|P-value:2.55E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:4.70|P-value:2.37E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:3.78|P-value:2.60E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:18.10|P-value:7.43E-85 ID:SGTC_168|Compound:3970-0862|FD-Score:4.10|P-value:5.56E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.62|P-value:4.09E-5 ID:SGTC_214|Compound:4373-3496|FD-Score:3.48|P-value:9.85E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:9.21|P-value:2.06E-23 ID:SGTC_310|Compound:1273-0058|FD-Score:3.32|P-value:1.97E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.87|P-value:8.75E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:3.47|P-value:1.04E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:-2.88|P-value:8.38E-4 ID:SGTC_448|Compound:monastrol|FD-Score:3.57|P-value:6.64E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.25|P-value:9.91E-12 ID:SGTC_514|Compound:4466-0038|FD-Score:7.24|P-value:3.82E-15 ID:SGTC_569|Compound:4130-2812|FD-Score:-3.01|P-value:5.16E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-5.11|P-value:1.47E-8 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.08|P-value:4.01E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:5.23|P-value:1.01E-8 ID:SGTC_676|Compound:falcarinol|FD-Score:3.88|P-value:1.65E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.59|P-value:6.26E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:-3.03|P-value:4.81E-4 ID:SGTC_812|Compound:4251-0442|FD-Score:3.42|P-value:1.29E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.17|P-value:3.51E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.21|P-value:3.00E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.04|P-value:5.81E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.04|P-value:7.64E-6 ID:SGTC_1093|Compound:fluphenazine|FD-Score:2.93|P-value:8.84E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:2.90|P-value:9.86E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.95|P-value:1.16E-5 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.09|P-value:4.79E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.66|P-value:4.60E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.39|P-value:1.14E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.33|P-value:1.89E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-4.23|P-value:2.15E-6 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-5.37|P-value:2.89E-9 ID:SGTC_1720|Compound:st037298|FD-Score:-2.98|P-value:5.80E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.24|P-value:2.13E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:9.62|P-value:2.21E-25 ID:SGTC_2004|Compound:4023728|FD-Score:3.48|P-value:9.78E-5 ID:SGTC_2063|Compound:5246145|FD-Score:-3.05|P-value:4.51E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.35|P-value:1.71E-4 ID:SGTC_2078|Compound:5212016|FD-Score:-3.04|P-value:4.67E-4 ID:SGTC_2080|Compound:5212524|FD-Score:2.90|P-value:9.96E-4 ID:SGTC_2111|Compound:5486399|FD-Score:3.29|P-value:2.19E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.26|P-value:2.52E-4 ID:SGTC_2174|Compound:5790901|FD-Score:3.06|P-value:5.47E-4 ID:SGTC_2186|Compound:6124449|FD-Score:5.72|P-value:4.26E-10 ID:SGTC_2190|Compound:6049624|FD-Score:3.23|P-value:2.80E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.98|P-value:1.01E-5 ID:SGTC_2373|Compound:9071805|FD-Score:3.11|P-value:4.40E-4 ID:SGTC_2381|Compound:9035592|FD-Score:4.42|P-value:1.08E-6 ID:SGTC_2384|Compound:9036245|FD-Score:3.36|P-value:1.62E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.38|P-value:1.49E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-5.22|P-value:7.59E-9 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.80|P-value:2.39E-5 ID:SGTC_2570|Compound:licareol|FD-Score:3.35|P-value:1.71E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.49|P-value:7.48E-7 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.53|P-value:7.94E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:8.65|P-value:6.97E-21 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-2.91|P-value:7.51E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:8.38|P-value:1.12E-19 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.13|P-value:4.13E-4 ID:SGTC_2695|Compound:st077686|FD-Score:-3.21|P-value:2.34E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.39|P-value:1.43E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.69|P-value:4.00E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:2.91|P-value:9.49E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.71|P-value:2.76E-5 ID:SGTC_3024|Compound:9090631|FD-Score:2.93|P-value:8.77E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-3.33|P-value:1.46E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.78|P-value:1.94E-5 ID:SGTC_3044|Compound:9093193|FD-Score:3.19|P-value:3.22E-4 ID:SGTC_3054|Compound:9094397|FD-Score:4.53|P-value:6.15E-7 ID:SGTC_3056|Compound:9094900|FD-Score:-2.89|P-value:8.28E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.56|P-value:5.16E-7 ID:SGTC_3153|Compound:9100258|FD-Score:4.09|P-value:6.04E-6 ID:SGTC_3180|Compound:9109287|FD-Score:3.08|P-value:4.98E-4

Top fitness defect scores for YDR049W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_159 3970-0959 18.10 50.0 µM 7.43E-85 1 1.72E-73
2 SGTC_1844 rapamycin 9.62 4.0 nM 2.21E-25 41 3.25E-22
3 SGTC_246 cycloheximide 9.21 667.0 µM 2.06E-23 2 1.63E-20
4 SGTC_2619 obliquin 8.65 87.9 µM 6.97E-21 cell wall 37 2.50E-18
5 SGTC_2679 rapamycin 8.38 1.0 nM 1.12E-19 37 2.75E-17
6 SGTC_514 4466-0038 7.24 2.6 nM 3.82E-15 cell wall 53 2.29E-13
7 SGTC_453 enantio-paf c16 6.25 10.0 µM 9.91E-12 37 2.06E-10
8 SGTC_58 0957-0393 5.76 9.0 µM 3.12E-10 cell wall 227 4.10E-9
9 SGTC_2186 6124449 5.72 200.0 µM 4.26E-10 RSC complex & mRNA processing 3 5.38E-9
10 SGTC_81 0411-0046 5.56 10.0 µM 1.23E-9 10 1.34E-8
11 SGTC_673 1082-0474 5.23 110.0 µM 1.01E-8 RSC complex & mRNA processing 32 8.39E-8
12 SGTC_312 4092-0653 4.87 6.8 µM 8.75E-8 ergosterol depletion effects on membrane 61 5.47E-7
13 SGTC_149 3970-0822 4.70 46.1 µM 2.37E-7 tubulin folding & SWR complex 55 1.30E-6
14 SGTC_3093 9117344 4.56 49.5 µM 5.16E-7 60S ribosome export 7 2.56E-6
15 SGTC_3054 9094397 4.53 49.5 µM 6.15E-7 fatty acid desaturase (OLE1) 17 2.98E-6
16 SGTC_2582 agaric acid 4.49 100.0 µM 7.48E-7 redox potentiating 258 3.53E-6
17 SGTC_2381 9035592 4.42 200.0 µM 1.08E-6 9 4.85E-6
18 SGTC_168 3970-0862 4.10 530.5 µM 5.56E-6 112 2.03E-5
19 SGTC_3153 9100258 4.09 49.5 µM 6.04E-6 16 2.18E-5
20 SGTC_916 4466-0038 4.04 42.0 nM 7.64E-6 cell wall 66 2.67E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.401 1.00E-129 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.354 1.80E-99 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.295 1.62E-68 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.293 1.29E-67 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.257 1.02E-51 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.255 3.97E-51 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.247 6.27E-48 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.247 1.07E-47 YOL054W PSH1 hom E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p
0.241 1.48E-45 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
0.237 3.54E-44 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.231 8.94E-42 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.230 2.94E-41 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.223 3.27E-39 YLL039C UBI4 hom Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress
0.223 6.11E-39 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.222 8.98E-39 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)