YDR066C / RTR2

Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR066C / RTR2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR066C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_794 k038-0031 6.07 316.0 µM 6.21E-10 ubiquinone biosynthesis & proteasome 57 6.33E-10
2 SGTC_2690 usnic acid 5.59 100.0 µM 1.14E-8 7 1.15E-8
3 SGTC_1124 fisetin 5.56 1.2 µM 1.34E-8 9 1.35E-8
4 SGTC_2780 5215298 5.07 71.4 µM 1.97E-7 13 1.97E-7
5 SGTC_523 1934-0222 4.89 13.7 µM 5.14E-7 16 5.14E-7
6 SGTC_1249 0481-0015 4.74 10.2 µM 1.08E-6 13 1.08E-6
7 SGTC_374 0880-0231 4.69 82.3 µM 1.38E-6 iron homeostasis 12 1.38E-6
8 SGTC_581 0958-0271 4.52 211.0 µM 3.11E-6 36 3.10E-6
9 SGTC_2730 imipramine 4.36 71.3 µM 6.52E-6 11 6.49E-6
10 SGTC_574 1315-0397 4.35 40.0 µM 6.74E-6 23 6.70E-6
11 SGTC_683 panaxydol 4.16 45.0 µM 1.57E-5 sphingolipid biosynthesis & PDR1 32 1.56E-5
12 SGTC_1246 0422-0085 4.03 105.0 µM 2.83E-5 28 2.81E-5
13 SGTC_382 3229-0966 4.03 23.2 µM 2.86E-5 17 2.84E-5
14 SGTC_2259 7964009 3.91 200.0 µM 4.74E-5 superoxide 30 4.69E-5
15 SGTC_1972 st070201 3.85 17.4 µM 5.93E-5 26 5.87E-5
16 SGTC_939 1165-0479 3.83 45.3 µM 6.56E-5 30 6.50E-5
17 SGTC_1227 3909-8010 3.82 46.6 µM 6.62E-5 22 6.56E-5
18 SGTC_258 haloperidol 3.80 50.8 µM 7.42E-5 fatty acid desaturase (OLE1) 37 7.35E-5
19 SGTC_687 k029-0044 3.77 34.9 µM 8.32E-5 PDR1 18 8.24E-5
20 SGTC_1437 4092-1038 3.67 11.6 µM 1.23E-4 24 1.22E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 2.92E-9 YOR277C_d YOR277C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
0.102 3.22E-9 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.097 2.03E-8 YIL100W_d YIL100W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A
0.084 1.23E-6 YPR050C_d YPR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.083 1.60E-6 YKL128C PMU1 hom Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
0.083 1.71E-6 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.082 1.89E-6 YJR057W CDC8 het Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
0.081 3.06E-6 YDL211C_p YDL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.080 3.25E-6 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.080 3.29E-6 YOL126C MDH2 hom Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
0.077 7.42E-6 YDR480W DIG2 hom MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p
0.077 8.12E-6 YDL119C_p YDL119C_p hom Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.077 9.15E-6 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.074 1.67E-5 YOL145C CTR9 hom Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
0.074 1.91E-5 YMR304C-A_d YMR304C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10