Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
Profile for YDR089W / YDR089W

Click on Significant Values for Screen Details

Top fitness defect scores for YDR089W deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_828 2074-0507 7.50 139.0 µM 4.18E-18 RPP1 & pyrimidine depletion 5 3.30E-14
2 SGTC_2429 5902844 5.37 64.4 µM 3.75E-10 7 3.99E-8
3 SGTC_929 2805-0021 4.40 398.0 µM 2.23E-7 12 5.36E-6
4 SGTC_3145 9085516 4.32 49.5 µM 3.72E-7 fatty acid desaturase (OLE1) 18 7.94E-6
5 SGTC_6 0844-0013 4.09 19.8 µM 1.34E-6 76 2.13E-5
6 SGTC_823 0417-1665 3.68 178.0 µM 1.23E-5 46 1.17E-4
7 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.62 3.7 µM 1.64E-5 111 1.46E-4
8 SGTC_2704 st078022 3.59 71.1 µM 1.91E-5 21 1.65E-4
9 SGTC_2487 5918373 3.41 44.0 µM 4.50E-5 32 3.19E-4
10 SGTC_2944 9067346 3.40 25.6 µM 4.73E-5 49 3.31E-4
11 SGTC_2482 5981709 3.40 142.7 µM 4.81E-5 PDR1 59 3.36E-4
12 SGTC_437 s-farnesyl-l-cysteine methyl ester 3.23 6.7 µM 1.07E-4 32 6.23E-4
13 SGTC_778 3456-2434 3.23 196.0 µM 1.07E-4 mitochondrial response to ROS 116 6.25E-4
14 SGTC_453 enantio-paf c16 3.21 10.0 µM 1.15E-4 218 6.61E-4
15 SGTC_2106 5357045 3.20 167.3 µM 1.23E-4 43 6.94E-4
16 SGTC_2355 9061122 3.18 103.1 µM 1.33E-4 31 7.39E-4
17 SGTC_2427 5545055 3.10 42.8 µM 1.88E-4 50 9.65E-4
18 SGTC_781 4335-2806 3.05 94.4 µM 2.38E-4 49 0.00116
19 SGTC_335 4077-0256 3.03 27.6 µM 2.51E-4 43 0.00121
20 SGTC_1262 0737-0114 2.97 49.6 µM 3.33E-4 65 0.00150

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.258 4.14E-52 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.195 4.71E-30 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.194 9.72E-30 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.184 7.47E-27 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.170 3.21E-23 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.169 9.13E-23 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.161 7.92E-21 YDR026C NSI1 hom RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
0.159 2.33E-20 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.150 2.00E-18 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.150 2.76E-18 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.145 2.59E-17 YJR068W RFC2 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.144 4.73E-17 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.143 8.47E-17 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.143 9.76E-17 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.132 1.95E-14 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress