YDR128W / MTC5

Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR128W / MTC5

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-2.82|P-value:8.54E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:5.84|P-value:4.15E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:2.95|P-value:5.25E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-2.96|P-value:4.85E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.70|P-value:1.95E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.14|P-value:2.38E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.46|P-value:5.97E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:2.79|P-value:9.68E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:2.78|P-value:9.91E-4 ID:SGTC_447|Compound:ml7|FD-Score:-3.02|P-value:3.90E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:-2.90|P-value:6.26E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:4.49|P-value:3.00E-7 ID:SGTC_727|Compound:3032-0951|FD-Score:4.15|P-value:1.93E-6 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.02|P-value:3.81E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:2.80|P-value:9.16E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.05|P-value:3.51E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-2.86|P-value:7.30E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.07|P-value:3.25E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-2.89|P-value:6.49E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-2.84|P-value:7.71E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.24|P-value:1.57E-4 ID:SGTC_2014|Compound:4013930|FD-Score:5.08|P-value:8.17E-9 ID:SGTC_2393|Compound:9031399|FD-Score:3.85|P-value:9.25E-6 ID:SGTC_2398|Compound:5228213|FD-Score:2.90|P-value:6.29E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.83|P-value:8.15E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.56|P-value:3.64E-5 ID:SGTC_2424|Compound:5537928|FD-Score:-4.73|P-value:7.04E-8 ID:SGTC_2428|Compound:5615643|FD-Score:4.55|P-value:2.08E-7 ID:SGTC_2431|Compound:5490079|FD-Score:4.27|P-value:1.05E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.03|P-value:3.71E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.42|P-value:8.15E-10 ID:SGTC_2692|Compound:st077426|FD-Score:4.21|P-value:1.45E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.29|P-value:1.22E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.76|P-value:1.44E-5 ID:SGTC_2926|Compound:9010726|FD-Score:4.00|P-value:4.24E-6 ID:SGTC_3173|Compound:9105693|FD-Score:4.27|P-value:1.02E-6 ID:SGTC_3177|Compound:9107506|FD-Score:3.17|P-value:2.16E-4 ID:SGTC_3185|Compound:9108267|FD-Score:-3.37|P-value:8.85E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-4.98|P-value:1.48E-8 ID:SGTC_3242|Compound:9133940|FD-Score:-3.11|P-value:2.72E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.64|P-value:2.54E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.19|P-value:1.56E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:-2.82|P-value:8.54E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:5.84|P-value:4.15E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:2.95|P-value:5.25E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-2.96|P-value:4.85E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.70|P-value:1.95E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:3.14|P-value:2.38E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.46|P-value:5.97E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:2.79|P-value:9.68E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:2.78|P-value:9.91E-4 ID:SGTC_447|Compound:ml7|FD-Score:-3.02|P-value:3.90E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:-2.90|P-value:6.26E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:4.49|P-value:3.00E-7 ID:SGTC_727|Compound:3032-0951|FD-Score:4.15|P-value:1.93E-6 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.02|P-value:3.81E-4 ID:SGTC_1081|Compound:sertraline|FD-Score:2.80|P-value:9.16E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:3.05|P-value:3.51E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-2.86|P-value:7.30E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.07|P-value:3.25E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-2.89|P-value:6.49E-4 ID:SGTC_1694|Compound:st024011|FD-Score:-2.84|P-value:7.71E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.24|P-value:1.57E-4 ID:SGTC_2014|Compound:4013930|FD-Score:5.08|P-value:8.17E-9 ID:SGTC_2393|Compound:9031399|FD-Score:3.85|P-value:9.25E-6 ID:SGTC_2398|Compound:5228213|FD-Score:2.90|P-value:6.29E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-2.83|P-value:8.15E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.56|P-value:3.64E-5 ID:SGTC_2424|Compound:5537928|FD-Score:-4.73|P-value:7.04E-8 ID:SGTC_2428|Compound:5615643|FD-Score:4.55|P-value:2.08E-7 ID:SGTC_2431|Compound:5490079|FD-Score:4.27|P-value:1.05E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.15|P-value:2.33E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.03|P-value:3.71E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.42|P-value:8.15E-10 ID:SGTC_2692|Compound:st077426|FD-Score:4.21|P-value:1.45E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.29|P-value:1.22E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.76|P-value:1.44E-5 ID:SGTC_2926|Compound:9010726|FD-Score:4.00|P-value:4.24E-6 ID:SGTC_3173|Compound:9105693|FD-Score:4.27|P-value:1.02E-6 ID:SGTC_3177|Compound:9107506|FD-Score:3.17|P-value:2.16E-4 ID:SGTC_3185|Compound:9108267|FD-Score:-3.37|P-value:8.85E-5 ID:SGTC_3212|Compound:9128307|FD-Score:-4.98|P-value:1.48E-8 ID:SGTC_3242|Compound:9133940|FD-Score:-3.11|P-value:2.72E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.64|P-value:2.54E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.19|P-value:1.56E-6

Top fitness defect scores for YDR128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_46 0329-0187a 5.84 2.2 µM 4.15E-11 29 2.62E-9
2 SGTC_2651 1327-0036 5.42 5.0 µM 8.15E-10 92 2.94E-8
3 SGTC_2014 4013930 5.08 200.0 µM 8.17E-9 7 1.92E-7
4 SGTC_2428 5615643 4.55 7.8 µM 2.08E-7 25 2.66E-6
5 SGTC_716 1683-5115 4.49 658.0 µM 3.00E-7 10 3.60E-6
6 SGTC_3173 9105693 4.27 49.5 µM 1.02E-6 13 9.77E-6
7 SGTC_2431 5490079 4.27 200.0 µM 1.05E-6 23 9.99E-6
8 SGTC_2692 st077426 4.21 47.7 µM 1.45E-6 19 1.30E-5
9 SGTC_3328 9140624 4.19 29.0 µM 1.56E-6 14 1.38E-5
10 SGTC_727 3032-0951 4.15 202.0 µM 1.93E-6 25 1.64E-5
11 SGTC_2926 9010726 4.00 65.2 µM 4.24E-6 33 3.11E-5
12 SGTC_2393 9031399 3.85 185.9 µM 9.25E-6 RNA processing & uracil transport 29 5.89E-5
13 SGTC_2837 9008476 3.76 45.5 µM 1.44E-5 42 8.46E-5
14 SGTC_169 k015-0017 3.70 16.0 µM 1.95E-5 25 1.08E-4
15 SGTC_3327 9140648 3.64 26.9 µM 2.54E-5 16 1.34E-4
16 SGTC_356 1634-0012 3.46 32.5 µM 5.97E-5 40 2.70E-4
17 SGTC_1702 st027038 3.24 51.0 µM 1.57E-4 36 5.95E-4
18 SGTC_3177 9107506 3.17 49.5 µM 2.16E-4 ERG2 17 7.75E-4
19 SGTC_2584 androsterone 3.15 48.7 µM 2.33E-4 cell wall 197 8.25E-4
20 SGTC_354 2783-3500 3.14 4.9 µM 2.38E-4 cell wall 86 8.39E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 7.58E-35 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.162 3.63E-21 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.159 2.01E-20 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.134 5.78E-15 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.123 9.62E-13 YJL133W MRS3 hom Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
0.121 2.37E-12 YIL101C XBP1 hom Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.116 1.44E-11 YGR044C RME1 hom Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.112 9.32E-11 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.110 1.57E-10 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.106 7.77E-10 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.103 2.58E-9 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.101 4.29E-9 YLR066W SPC3 het Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
0.101 4.69E-9 YDR278C_d YDR278C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.101 5.43E-9 YGR092W DBF2 hom Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
0.100 6.53E-9 YGL100W SEH1 hom Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1