HIPHOP chemogenomics database

YDR170C / SEC7

Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR170C / SEC7

Click on Significant Values for Screen Details

Top fitness defect scores for YDR170C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_999	4022-0066	47.40	22.7 µM	0	Golgi	1	0	39.50
2	SGTC_2242	6630449	40.20	61.3 µM	0	Golgi	1	0	32.60
3	SGTC_3004	9076122	7.54	71.4 µM	1.56E-14		1	2.39E-14	1.97
4	SGTC_3037	9091026	5.22	49.5 µM	7.51E-8	NEO1-PIK1	16	9.05E-8	0.44
5	SGTC_2657	nystatin	4.70	74.4 µM	1.14E-6		1	1.33E-6	0.38
6	SGTC_2541	muurolladie-3-one	4.68	83.1 µM	1.22E-6	excess fatty acid	15	1.41E-6	0.33
7	SGTC_514	4466-0038	4.50	2.6 nM	2.91E-6	cell wall	32	3.33E-6	0.17
8	SGTC_1800	st050079	4.34	29.1 µM	6.16E-6	NEO1-PIK1	20	6.96E-6	0.01
9	SGTC_780	0109-0199	4.31	192.0 µM	7.20E-6		3	8.12E-6	0.48
10	SGTC_1838	st055369	4.28	84.5 µM	8.11E-6		4	9.14E-6	0.14
11	SGTC_2321	7390090	4.22	138.8 µM	1.09E-5		3	1.23E-5	0.08
12	SGTC_3159	9100096	4.21	49.5 µM	1.16E-5		4	1.30E-5	0.26
13	SGTC_1785	bathophenanthroline	4.12	24.0 µM	1.71E-5	heme requiring	21	1.91E-5	0.06
14	SGTC_1595	6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin	3.80	67.5 µM	6.73E-5		10	7.36E-5	0.02
15	SGTC_2428	5615643	3.76	7.8 µM	7.79E-5		7	8.50E-5	0.29
16	SGTC_2336	9001819	3.72	198.7 µM	9.03E-5	heme biosynthesis & mitochondrial translocase	42	9.83E-5	0.09
17	SGTC_1203	3992-3522	3.59	103.0 µM	1.54E-4		18	1.67E-4	0.10
18	SGTC_1556	plumbagin	3.56	1.5 µM	1.70E-4	superoxide	24	1.83E-4	0.03
19	SGTC_3013	9080133	3.54	71.4 µM	1.88E-4		4	2.02E-4	0.07
20	SGTC_3115	9123671	3.42	49.5 µM	2.89E-4		4	3.10E-4	0.23

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.232	5.25E-42	YJL124C	LSM1	hom	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.229	4.53E-41	YBR164C	ARL1	hom	Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.224	2.15E-39	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.206	1.23E-33	YNR051C	BRE5	hom	Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
0.179	1.48E-25	YDR389W	SAC7	hom	GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.160	9.73E-21	YPL051W	ARL3	hom	GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.153	5.54E-19	YEL022W	GEA2	hom	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.150	2.81E-18	YDL031W	DBP10	het	Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
0.144	5.27E-17	YCR006C_d	YCR006C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.140	3.58E-16	YNL147W	LSM7	hom	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
0.134	7.64E-15	YKR001C	VPS1	hom	Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.130	4.95E-14	YNL053W	MSG5	hom	Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p
0.127	1.41E-13	YCR021C	HSP30	hom	Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
0.127	1.81E-13	YKR040C_d	YKR040C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
0.121	2.30E-12	YDR162C	NBP2	hom	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)