YDR206W / EBS1

Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR206W / EBS1

Click on Significant Values for Screen Details ID:SGTC_281|Compound:1672-3440|FD-Score:3.17|P-value:9.18E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.72|P-value:1.14E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.56|P-value:2.38E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.23|P-value:1.59E-5 ID:SGTC_455|Compound:paf C-16|FD-Score:3.26|P-value:6.90E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.26|P-value:6.86E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-3.23|P-value:6.78E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.43|P-value:3.35E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.86|P-value:6.63E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.29|P-value:5.64E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.15|P-value:9.80E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:-4.36|P-value:7.86E-6 ID:SGTC_1746|Compound:st038117|FD-Score:-3.62|P-value:1.70E-4 ID:SGTC_1747|Compound:st037496|FD-Score:9.23|P-value:4.85E-20 ID:SGTC_1753|Compound:st043925|FD-Score:3.33|P-value:5.36E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:-3.49|P-value:2.77E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.69|P-value:1.31E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.84|P-value:8.15E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.66|P-value:1.45E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.96|P-value:4.88E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.79|P-value:8.82E-5 ID:SGTC_2935|Compound:9038016|FD-Score:-3.22|P-value:7.22E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:3.17|P-value:9.18E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.72|P-value:1.14E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.56|P-value:2.38E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.23|P-value:1.59E-5 ID:SGTC_455|Compound:paf C-16|FD-Score:3.26|P-value:6.90E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.26|P-value:6.86E-4 ID:SGTC_512|Compound:prima-1|FD-Score:-3.23|P-value:6.78E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.43|P-value:3.35E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.86|P-value:6.63E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.29|P-value:5.64E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.15|P-value:9.80E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:-4.36|P-value:7.86E-6 ID:SGTC_1746|Compound:st038117|FD-Score:-3.62|P-value:1.70E-4 ID:SGTC_1747|Compound:st037496|FD-Score:9.23|P-value:4.85E-20 ID:SGTC_1753|Compound:st043925|FD-Score:3.33|P-value:5.36E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:-3.49|P-value:2.77E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.69|P-value:1.31E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.84|P-value:8.15E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.66|P-value:1.45E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.96|P-value:4.88E-5 ID:SGTC_2933|Compound:9026794|FD-Score:-3.79|P-value:8.82E-5 ID:SGTC_2935|Compound:9038016|FD-Score:-3.22|P-value:7.22E-4

Top fitness defect scores for YDR206W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1747 st037496 9.23 66.6 µM 4.85E-20 2 1.35E-20
2 SGTC_431 k089-0097 4.23 107.0 µM 1.59E-5 iron homeostasis 32 1.17E-5
3 SGTC_2930 9013230 3.96 14.9 µM 4.88E-5 heme biosynthesis & mitochondrial translocase 37 3.69E-5
4 SGTC_2762 leflunomide 3.84 42.1 µM 8.15E-5 36 6.26E-5
5 SGTC_374 0880-0231 3.56 82.3 µM 2.38E-4 iron homeostasis 35 1.89E-4
6 SGTC_1753 st043925 3.33 64.0 µM 5.36E-4 21 4.36E-4
7 SGTC_479 n-linoleoylglycine 3.26 10.0 µM 6.86E-4 22 5.61E-4
8 SGTC_455 paf C-16 3.26 10.0 µM 6.90E-4 53 5.65E-4
9 SGTC_281 1672-3440 3.17 14.9 µM 9.18E-4 Golgi 23 7.57E-4
10 SGTC_1255 0566-0045 3.15 221.0 µM 9.80E-4 excess fatty acid 61 8.11E-4
11 SGTC_1740 st038286 3.12 13.2 µM 0.00110 68 9.16E-4
12 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.10 3.7 µM 0.00118 185 9.81E-4
13 SGTC_1262 0737-0114 3.03 49.6 µM 0.00147 59 0.00123
14 SGTC_457 grayanotoxin iii 2.94 135.0 µM 0.00196 148 0.00166
15 SGTC_1412 3909-8563 2.93 178.0 µM 0.00199 52 0.00168
16 SGTC_3302 9125395 2.90 16.4 µM 0.00222 58 0.00188
17 SGTC_508 roscovitine 2.85 141.0 µM 0.00260 33 0.00221
18 SGTC_275 trichlorophene 2.81 5.7 µM 0.00287 mitochondrial stress 320 0.00245
19 SGTC_1057 3448-5381 2.74 134.0 µM 0.00362 calcium & mitochondrial duress 88 0.00311
20 SGTC_393 acivicin 2.73 6.2 µM 0.00367 66 0.00315

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.208 9.47E-34 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.203 1.21E-32 YDL220C CDC13 het Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
0.175 2.53E-24 YDR171W HSP42 hom Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
0.170 3.63E-23 YPL228W CET1 het Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
0.163 1.73E-21 YEL040W UTR2 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
0.155 1.40E-19 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.153 6.41E-19 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.150 2.11E-18 YDR143C SAN1 hom Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
0.138 8.49E-16 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.132 1.40E-14 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.131 2.64E-14 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.131 3.10E-14 YLR136C TIS11 hom mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.127 1.40E-13 YLR319C BUD6 hom Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
0.125 3.58E-13 YLR186W EMG1 het Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
0.124 5.40E-13 YGR074W SMD1 het Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress