YDR257C / RKM4

Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR257C / RKM4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.42|P-value:1.97E-13 ID:SGTC_143|Compound:4032-1194|FD-Score:4.70|P-value:5.39E-8 ID:SGTC_148|Compound:3253-0744|FD-Score:-4.21|P-value:9.78E-7 ID:SGTC_325|Compound:2810-4341|FD-Score:3.32|P-value:8.76E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:-3.21|P-value:1.45E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:2.87|P-value:5.92E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.66|P-value:1.75E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.02|P-value:3.15E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.90|P-value:5.29E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.85|P-value:6.64E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:-3.48|P-value:4.10E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:2.91|P-value:5.04E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.21|P-value:1.41E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.57|P-value:2.67E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.86|P-value:6.55E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.12|P-value:2.10E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.45|P-value:4.83E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.24|P-value:1.24E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.91|P-value:5.00E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:-2.98|P-value:3.84E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:2.79|P-value:7.99E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:2.76|P-value:9.11E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-2.81|P-value:7.43E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-2.78|P-value:8.43E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.84|P-value:7.17E-6 ID:SGTC_1705|Compound:st027868|FD-Score:-3.16|P-value:1.79E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.48|P-value:4.18E-5 ID:SGTC_1786|Compound:berberine|FD-Score:-2.77|P-value:8.77E-4 ID:SGTC_1814|Compound:st052110|FD-Score:2.78|P-value:8.31E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.09|P-value:2.38E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.10|P-value:2.33E-4 ID:SGTC_1852|Compound:st056254|FD-Score:2.96|P-value:4.01E-4 ID:SGTC_1861|Compound:st057647|FD-Score:2.85|P-value:6.32E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.03|P-value:3.02E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.72|P-value:4.74E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:2.87|P-value:5.81E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.18|P-value:1.60E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.30|P-value:9.63E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.09|P-value:2.37E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.90|P-value:5.18E-6 ID:SGTC_2351|Compound:9026858|FD-Score:-2.85|P-value:6.50E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.43|P-value:2.71E-7 ID:SGTC_2447|Compound:5741742|FD-Score:3.33|P-value:8.13E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-3.53|P-value:3.26E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-5.04|P-value:6.18E-9 ID:SGTC_2576|Compound:guaiol|FD-Score:2.93|P-value:4.61E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.22|P-value:9.04E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.47|P-value:4.38E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.57|P-value:2.68E-5 ID:SGTC_2699|Compound:st077704|FD-Score:2.86|P-value:6.05E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.13|P-value:2.04E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:2.76|P-value:8.86E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.10|P-value:2.74E-12 ID:SGTC_2782|Compound:7736369|FD-Score:-3.13|P-value:1.99E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.40|P-value:6.15E-5 ID:SGTC_2814|Compound:7977482|FD-Score:3.76|P-value:1.05E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:6.42|P-value:1.97E-13 ID:SGTC_143|Compound:4032-1194|FD-Score:4.70|P-value:5.39E-8 ID:SGTC_148|Compound:3253-0744|FD-Score:-4.21|P-value:9.78E-7 ID:SGTC_325|Compound:2810-4341|FD-Score:3.32|P-value:8.76E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:-3.21|P-value:1.45E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:2.87|P-value:5.92E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.66|P-value:1.75E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.02|P-value:3.15E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:2.90|P-value:5.29E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.85|P-value:6.64E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:-3.48|P-value:4.10E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:2.91|P-value:5.04E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.21|P-value:1.41E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.57|P-value:2.67E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.86|P-value:6.55E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.12|P-value:2.10E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-3.45|P-value:4.83E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.24|P-value:1.24E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.91|P-value:5.00E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:-2.98|P-value:3.84E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:2.79|P-value:7.99E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:2.76|P-value:9.11E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-2.81|P-value:7.43E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-2.78|P-value:8.43E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.84|P-value:7.17E-6 ID:SGTC_1705|Compound:st027868|FD-Score:-3.16|P-value:1.79E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.48|P-value:4.18E-5 ID:SGTC_1786|Compound:berberine|FD-Score:-2.77|P-value:8.77E-4 ID:SGTC_1814|Compound:st052110|FD-Score:2.78|P-value:8.31E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.09|P-value:2.38E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-3.10|P-value:2.33E-4 ID:SGTC_1852|Compound:st056254|FD-Score:2.96|P-value:4.01E-4 ID:SGTC_1861|Compound:st057647|FD-Score:2.85|P-value:6.32E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.03|P-value:3.02E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.72|P-value:4.74E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:2.87|P-value:5.81E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.18|P-value:1.60E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-3.30|P-value:9.63E-5 ID:SGTC_2333|Compound:9017922|FD-Score:3.09|P-value:2.37E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.90|P-value:5.18E-6 ID:SGTC_2351|Compound:9026858|FD-Score:-2.85|P-value:6.50E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.43|P-value:2.71E-7 ID:SGTC_2447|Compound:5741742|FD-Score:3.33|P-value:8.13E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-3.53|P-value:3.26E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-5.04|P-value:6.18E-9 ID:SGTC_2576|Compound:guaiol|FD-Score:2.93|P-value:4.61E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.22|P-value:9.04E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.47|P-value:4.38E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.57|P-value:2.68E-5 ID:SGTC_2699|Compound:st077704|FD-Score:2.86|P-value:6.05E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.13|P-value:2.04E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:2.76|P-value:8.86E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.10|P-value:2.74E-12 ID:SGTC_2782|Compound:7736369|FD-Score:-3.13|P-value:1.99E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.40|P-value:6.15E-5 ID:SGTC_2814|Compound:7977482|FD-Score:3.76|P-value:1.05E-5

Top fitness defect scores for YDR257C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 6.42 9.0 µM 1.97E-13 cell wall 187 6.88E-11
2 SGTC_2752 diclazuril 6.10 33.1 µM 2.74E-12 55 5.44E-10
3 SGTC_2111 5486399 4.72 200.0 µM 4.74E-8 21 1.18E-6
4 SGTC_143 4032-1194 4.70 15.7 µM 5.39E-8 36 1.31E-6
5 SGTC_2444 5485484 4.43 36.4 µM 2.71E-7 23 4.68E-6
6 SGTC_2582 agaric acid 4.22 100.0 µM 9.04E-7 redox potentiating 292 1.21E-5
7 SGTC_2346 9031566 3.90 143.8 µM 5.18E-6 Golgi 18 4.82E-5
8 SGTC_1606 st000094 3.84 49.2 µM 7.17E-6 60S ribosome export 22 6.23E-5
9 SGTC_2814 7977482 3.76 43.0 µM 1.05E-5 58 8.46E-5
10 SGTC_1779 st039427 3.48 55.0 µM 4.18E-5 45 2.52E-4
11 SGTC_2651 1327-0036 3.47 5.0 µM 4.38E-5 200 2.61E-4
12 SGTC_2794 7785382 3.40 27.3 µM 6.15E-5 57 3.42E-4
13 SGTC_2447 5741742 3.33 33.8 µM 8.13E-5 49 4.27E-4
14 SGTC_325 2810-4341 3.32 181.0 µM 8.76E-5 amide catabolism 19 4.53E-4
15 SGTC_923 1900-2434 3.21 73.3 µM 1.41E-4 RNA processing & uracil transport 56 6.61E-4
16 SGTC_2130 NSC 7 3.18 2.5 µM 1.60E-4 copper-dependent oxidative stress 74 7.31E-4
17 SGTC_1125 tnp00248 3.12 1.3 µM 2.10E-4 92 9.07E-4
18 SGTC_2333 9017922 3.09 200.0 µM 2.37E-4 62 0.00100
19 SGTC_1941 st076221 3.03 61.8 µM 3.02E-4 sphingolipid biosynthesis & PDR1 140 0.00121
20 SGTC_1852 st056254 2.96 31.4 µM 4.01E-4 47 0.00152

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.246 1.39E-47 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.226 4.07E-40 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.214 5.86E-36 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.203 1.79E-32 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.200 1.58E-31 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.190 1.67E-28 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.178 3.18E-25 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.176 1.21E-24 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.173 4.65E-24 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.168 9.80E-23 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.166 3.20E-22 YNL135C FPR1 hom Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function
0.166 3.63E-22 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.166 4.30E-22 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.162 4.26E-21 YOR141C ARP8 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
0.162 4.66E-21 YIL123W SIM1 hom Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated