YDR258C / HSP78

Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR258C / HSP78

Click on Significant Values for Screen Details ID:SGTC_148|Compound:3253-0744|FD-Score:-3.04|P-value:2.96E-4 ID:SGTC_190|Compound:3970-0790|FD-Score:3.60|P-value:2.89E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:-3.20|P-value:1.52E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-2.99|P-value:3.67E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.00|P-value:4.09E-6 ID:SGTC_263|Compound:alizarin|FD-Score:2.93|P-value:5.52E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:3.21|P-value:1.75E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.03|P-value:3.12E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-2.94|P-value:4.44E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:5.15|P-value:4.32E-9 ID:SGTC_435|Compound:c-PAF|FD-Score:3.50|P-value:4.73E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:2.98|P-value:4.47E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.79|P-value:1.19E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:2.78|P-value:9.63E-4 ID:SGTC_918|Compound:glabranin|FD-Score:-4.06|P-value:2.38E-6 ID:SGTC_1179|Compound:1021-1431|FD-Score:2.82|P-value:8.40E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.20|P-value:1.81E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:2.90|P-value:6.09E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-2.81|P-value:7.58E-4 ID:SGTC_1518|Compound:st038567|FD-Score:-2.96|P-value:4.09E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.16|P-value:1.78E-4 ID:SGTC_1640|Compound:st009078|FD-Score:2.87|P-value:6.95E-4 ID:SGTC_1662|Compound:st014054|FD-Score:-3.17|P-value:1.75E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.68|P-value:1.97E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.92|P-value:1.47E-8 ID:SGTC_1963|Compound:st076565|FD-Score:-3.43|P-value:5.37E-5 ID:SGTC_1973|Compound:st070275|FD-Score:2.80|P-value:8.98E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.05|P-value:3.39E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-2.90|P-value:5.35E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.43|P-value:6.47E-5 ID:SGTC_2006|Compound:4011487|FD-Score:10.60|P-value:7.11E-33 ID:SGTC_2008|Compound:4012253|FD-Score:-4.68|P-value:6.83E-8 ID:SGTC_2011|Compound:4012611|FD-Score:-6.89|P-value:4.28E-15 ID:SGTC_2087|Compound:5276107|FD-Score:-2.94|P-value:4.47E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-3.19|P-value:1.54E-4 ID:SGTC_2153|Compound:5569715|FD-Score:4.19|P-value:1.48E-6 ID:SGTC_2516|Compound:gossypol|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.79|P-value:9.53E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.56|P-value:3.00E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.08|P-value:3.02E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.17|P-value:2.03E-4 ID:SGTC_2844|Compound:9010103|FD-Score:3.41|P-value:7.26E-5 ID:SGTC_2848|Compound:9016370|FD-Score:4.51|P-value:2.38E-7 ID:SGTC_2997|Compound:9067075|FD-Score:5.84|P-value:3.40E-11 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.04|P-value:2.96E-4 ID:SGTC_190|Compound:3970-0790|FD-Score:3.60|P-value:2.89E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:-3.20|P-value:1.52E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-2.99|P-value:3.67E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.00|P-value:4.09E-6 ID:SGTC_263|Compound:alizarin|FD-Score:2.93|P-value:5.52E-4 ID:SGTC_323|Compound:4013-0906|FD-Score:3.21|P-value:1.75E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.03|P-value:3.12E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-2.94|P-value:4.44E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:5.15|P-value:4.32E-9 ID:SGTC_435|Compound:c-PAF|FD-Score:3.50|P-value:4.73E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:2.98|P-value:4.47E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.79|P-value:1.19E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:2.78|P-value:9.63E-4 ID:SGTC_918|Compound:glabranin|FD-Score:-4.06|P-value:2.38E-6 ID:SGTC_1179|Compound:1021-1431|FD-Score:2.82|P-value:8.40E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.20|P-value:1.81E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:2.90|P-value:6.09E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-2.81|P-value:7.58E-4 ID:SGTC_1518|Compound:st038567|FD-Score:-2.96|P-value:4.09E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.16|P-value:1.78E-4 ID:SGTC_1640|Compound:st009078|FD-Score:2.87|P-value:6.95E-4 ID:SGTC_1662|Compound:st014054|FD-Score:-3.17|P-value:1.75E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.68|P-value:1.97E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.92|P-value:1.47E-8 ID:SGTC_1963|Compound:st076565|FD-Score:-3.43|P-value:5.37E-5 ID:SGTC_1973|Compound:st070275|FD-Score:2.80|P-value:8.98E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.05|P-value:3.39E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-2.90|P-value:5.35E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.43|P-value:6.47E-5 ID:SGTC_2006|Compound:4011487|FD-Score:10.60|P-value:7.11E-33 ID:SGTC_2008|Compound:4012253|FD-Score:-4.68|P-value:6.83E-8 ID:SGTC_2011|Compound:4012611|FD-Score:-6.89|P-value:4.28E-15 ID:SGTC_2087|Compound:5276107|FD-Score:-2.94|P-value:4.47E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-3.19|P-value:1.54E-4 ID:SGTC_2153|Compound:5569715|FD-Score:4.19|P-value:1.48E-6 ID:SGTC_2516|Compound:gossypol|FD-Score:3.21|P-value:1.70E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.79|P-value:9.53E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.56|P-value:3.00E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.08|P-value:3.02E-4 ID:SGTC_2839|Compound:9003951|FD-Score:3.17|P-value:2.03E-4 ID:SGTC_2844|Compound:9010103|FD-Score:3.41|P-value:7.26E-5 ID:SGTC_2848|Compound:9016370|FD-Score:4.51|P-value:2.38E-7 ID:SGTC_2997|Compound:9067075|FD-Score:5.84|P-value:3.40E-11

Top fitness defect scores for YDR258C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2006 4011487 10.60 200.0 µM 7.11E-33 endomembrane recycling 15 1.32E-26
2 SGTC_2997 9067075 5.84 71.4 µM 3.40E-11 unfolded protein response 21 2.68E-9
3 SGTC_431 k089-0097 5.15 107.0 µM 4.32E-9 iron homeostasis 10 1.31E-7
4 SGTC_2848 9016370 4.51 26.0 µM 2.38E-7 13 3.27E-6
5 SGTC_2153 5569715 4.19 200.0 µM 1.48E-6 iron homeostasis 17 1.43E-5
6 SGTC_252 miconazole 4.00 1.3 nM 4.09E-6 ergosterol depletion effects on membrane 34 3.23E-5
7 SGTC_685 1278-0033 3.79 54.9 µM 1.19E-5 60S ribosome export 25 7.62E-5
8 SGTC_1748 st037455 3.68 53.6 µM 1.97E-5 38 1.15E-4
9 SGTC_190 3970-0790 3.60 3.7 µM 2.89E-5 13 1.56E-4
10 SGTC_435 c-PAF 3.50 6.7 µM 4.73E-5 66 2.33E-4
11 SGTC_2002 4023503 3.43 131.0 µM 6.47E-5 mitochondrial processes 59 3.00E-4
12 SGTC_2844 9010103 3.41 26.0 µM 7.26E-5 51 3.30E-4
13 SGTC_2516 gossypol 3.21 71.5 µM 1.70E-4 44 6.56E-4
14 SGTC_323 4013-0906 3.21 34.3 µM 1.75E-4 ERG2 26 6.71E-4
15 SGTC_1370 2001-0092 3.20 44.6 µM 1.81E-4 74 6.92E-4
16 SGTC_2839 9003951 3.17 26.0 µM 2.03E-4 38 7.57E-4
17 SGTC_2810 7959871 3.08 45.5 µM 3.02E-4 copper-dependent oxidative stress 84 0.00105
18 SGTC_1976 st070609 3.05 63.5 µM 3.39E-4 37 0.00115
19 SGTC_669 0108-0021 2.98 26.2 µM 4.47E-4 fatty acid desaturase (OLE1) 151 0.00144
20 SGTC_263 alizarin 2.93 107.9 µM 5.52E-4 iron homeostasis 134 0.00171

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.207 8.24E-34 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.167 2.10E-22 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.154 3.47E-19 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.149 3.52E-18 YGR042W YGR042W hom Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
0.144 4.77E-17 YGL199C_d YGL199C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
0.142 1.37E-16 YNL135C FPR1 hom Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function
0.135 3.28E-15 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.134 6.84E-15 YNL293W MSB3 hom Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.132 1.55E-14 YFL034C-A RPL22B hom Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
0.131 3.82E-14 YDL090C RAM1 hom Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.129 5.78E-14 YBR014C GRX7 hom Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
0.128 1.21E-13 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.120 2.89E-12 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.120 3.07E-12 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.118 7.43E-12 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism