YDR321W / ASP1

Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR321W / ASP1

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:5.01|P-value:2.97E-7 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.14|P-value:1.84E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:3.63|P-value:1.43E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.34|P-value:4.26E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:-4.99|P-value:4.31E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:4.68|P-value:1.55E-6 ID:SGTC_362|Compound:0310-0100|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:4.01|P-value:3.11E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:3.77|P-value:8.51E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.38|P-value:3.74E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:4.09|P-value:2.28E-5 ID:SGTC_715|Compound:0868-0130|FD-Score:3.40|P-value:3.45E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.19|P-value:7.20E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.15|P-value:8.28E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:6.09|P-value:6.69E-10 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.33|P-value:4.40E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.18|P-value:7.46E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.20|P-value:1.51E-7 ID:SGTC_1731|Compound:st035964|FD-Score:3.78|P-value:7.95E-5 ID:SGTC_1750|Compound:st081859|FD-Score:-3.50|P-value:2.95E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-4.74|P-value:1.48E-6 ID:SGTC_1899|Compound:st063254|FD-Score:3.94|P-value:4.18E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.24|P-value:7.20E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.32|P-value:5.57E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.14|P-value:8.48E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-4.72|P-value:1.65E-6 ID:SGTC_2279|Compound:7948423|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.57|P-value:1.81E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.11|P-value:9.37E-4 ID:SGTC_2404|Compound:5161160|FD-Score:-4.15|P-value:2.24E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.92|P-value:4.68E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.86|P-value:7.18E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.44|P-value:2.93E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-6.34|P-value:1.99E-10 ID:SGTC_2785|Compound:7756463|FD-Score:-7.85|P-value:4.68E-15 ID:SGTC_2795|Compound:7780010|FD-Score:-4.01|P-value:4.05E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.86|P-value:5.88E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-4.03|P-value:3.59E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-3.66|P-value:1.60E-4 ID:SGTC_2926|Compound:9010726|FD-Score:4.03|P-value:2.91E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.81|P-value:7.12E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.31|P-value:5.68E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.96|P-value:3.96E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.34|P-value:5.06E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.36|P-value:3.89E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:5.01|P-value:2.97E-7 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.14|P-value:1.84E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:3.63|P-value:1.43E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.34|P-value:4.26E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:-4.99|P-value:4.31E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:4.68|P-value:1.55E-6 ID:SGTC_362|Compound:0310-0100|FD-Score:4.27|P-value:1.05E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:4.01|P-value:3.11E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:3.77|P-value:8.51E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.38|P-value:3.74E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:4.09|P-value:2.28E-5 ID:SGTC_715|Compound:0868-0130|FD-Score:3.40|P-value:3.45E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.19|P-value:7.20E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.15|P-value:8.28E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:6.09|P-value:6.69E-10 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.33|P-value:4.40E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.18|P-value:7.46E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.20|P-value:1.51E-7 ID:SGTC_1731|Compound:st035964|FD-Score:3.78|P-value:7.95E-5 ID:SGTC_1750|Compound:st081859|FD-Score:-3.50|P-value:2.95E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-4.74|P-value:1.48E-6 ID:SGTC_1899|Compound:st063254|FD-Score:3.94|P-value:4.18E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.24|P-value:7.20E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.32|P-value:5.57E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.14|P-value:8.48E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-4.72|P-value:1.65E-6 ID:SGTC_2279|Compound:7948423|FD-Score:3.10|P-value:9.78E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.57|P-value:1.81E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.11|P-value:9.37E-4 ID:SGTC_2404|Compound:5161160|FD-Score:-4.15|P-value:2.24E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.92|P-value:4.68E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.86|P-value:7.18E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.44|P-value:2.93E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-6.34|P-value:1.99E-10 ID:SGTC_2785|Compound:7756463|FD-Score:-7.85|P-value:4.68E-15 ID:SGTC_2795|Compound:7780010|FD-Score:-4.01|P-value:4.05E-5 ID:SGTC_2799|Compound:7812892|FD-Score:3.86|P-value:5.88E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-4.03|P-value:3.59E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-3.66|P-value:1.60E-4 ID:SGTC_2926|Compound:9010726|FD-Score:4.03|P-value:2.91E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.81|P-value:7.12E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.31|P-value:5.68E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.96|P-value:3.96E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.34|P-value:5.06E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.36|P-value:3.89E-4

Top fitness defect scores for YDR321W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 6.09 1.8 µM 6.69E-10 34 5.57E-10
2 SGTC_8 0354-0001 5.01 70.0 µM 2.97E-7 43 2.69E-7
3 SGTC_245 methotrexate 4.68 289.6 µM 1.55E-6 26 1.43E-6
4 SGTC_362 0310-0100 4.27 9.0 µM 1.05E-5 RPP1 & pyrimidine depletion 26 9.91E-6
5 SGTC_46 0329-0187a 4.14 2.2 µM 1.84E-5 68 1.75E-5
6 SGTC_526 4469-0568 4.09 170.0 µM 2.28E-5 azole & statin 32 2.18E-5
7 SGTC_2926 9010726 4.03 65.2 µM 2.91E-5 32 2.79E-5
8 SGTC_363 0455-0021 4.01 3.1 µM 3.11E-5 azole & statin 64 2.98E-5
9 SGTC_2991 9074669 3.96 71.4 µM 3.96E-5 azole & statin 63 3.81E-5
10 SGTC_1899 st063254 3.94 59.6 µM 4.18E-5 14 4.02E-5
11 SGTC_2563 tomatine 3.92 8.5 µM 4.68E-5 48 4.51E-5
12 SGTC_2799 7812892 3.86 81.8 µM 5.88E-5 superoxide 31 5.68E-5
13 SGTC_2929 9013348 3.81 24.6 µM 7.12E-5 26 6.89E-5
14 SGTC_1731 st035964 3.78 63.0 µM 7.95E-5 34 7.71E-5
15 SGTC_371 0195-0006 3.77 7.4 µM 8.51E-5 23 8.25E-5
16 SGTC_212 3807-4682 3.63 38.8 µM 1.43E-4 tubulin folding & SWR complex 87 1.40E-4
17 SGTC_2381 9035592 3.57 200.0 µM 1.81E-4 30 1.77E-4
18 SGTC_2662 daunorubicin 3.44 22.4 µM 2.93E-4 anthracycline transcription coupled DNA repair 26 2.88E-4
19 SGTC_715 0868-0130 3.40 184.0 µM 3.45E-4 18 3.40E-4
20 SGTC_470 aa-861 3.38 153.0 µM 3.74E-4 sphingolipid biosynthesis & PDR1 82 3.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.211 3.40E-35 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.210 9.24E-35 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.154 2.73E-19 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.143 7.82E-17 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.141 2.84E-16 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.140 4.16E-16 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.138 1.27E-15 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.136 2.43E-15 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.126 2.39E-13 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.126 2.56E-13 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.123 8.80E-13 YDR278C_d YDR278C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.120 3.16E-12 YDR098C GRX3 hom Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36
0.115 2.04E-11 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.113 5.95E-11 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.113 6.20E-11 YPL133C RDS2 hom Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole