YDR352W / YPQ2

Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR352W / YPQ2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR352W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2042 p-Dimethylaminoacetophenone 4.56 200.0 µM 1.35E-7 7 2.52E-6
2 SGTC_246 cycloheximide 4.54 667.0 µM 1.54E-7 8 2.80E-6
3 SGTC_966 1300-0360 4.41 30.2 µM 3.41E-7 heme requiring 17 5.26E-6
4 SGTC_2354 9060492 4.04 162.0 µM 2.71E-6 15 2.72E-5
5 SGTC_3217 9129673 4.00 49.5 µM 3.33E-6 37 3.21E-5
6 SGTC_1009 4106-0057 3.77 9.9 µM 1.07E-5 17 8.14E-5
7 SGTC_959 1222-0044 3.65 107.0 µM 1.92E-5 27 1.29E-4
8 SGTC_1716 st033144 3.63 30.6 µM 2.15E-5 29 1.41E-4
9 SGTC_933 2914-0560 3.46 114.0 µM 4.79E-5 40 2.68E-4
10 SGTC_1008 4106-0037 3.35 6.0 µM 8.19E-5 PDR1 32 4.11E-4
11 SGTC_3347 9188721 3.24 44.7 µM 1.29E-4 42 5.92E-4
12 SGTC_1370 2001-0092 3.16 44.6 µM 1.88E-4 77 7.98E-4
13 SGTC_1833 st055984 3.15 71.0 µM 1.97E-4 47 8.28E-4
14 SGTC_1203 3992-3522 3.06 103.0 µM 2.87E-4 82 0.00112
15 SGTC_2586 curcumin 3.04 80.0 µM 3.05E-4 iron homeostasis 112 0.00118
16 SGTC_2356 9061756 3.04 189.0 µM 3.06E-4 33 0.00118
17 SGTC_2046 4110841 2.98 106.0 µM 3.91E-4 iron homeostasis 65 0.00143
18 SGTC_3295 9122362 2.98 53.6 µM 3.91E-4 56 0.00143
19 SGTC_1908 st060353 2.95 56.5 µM 4.37E-4 RPP1 & pyrimidine depletion 90 0.00157
20 SGTC_1717 st033143 2.93 58.0 µM 4.76E-4 102 0.00168

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 2.11E-17 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.139 6.44E-16 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed
0.130 4.13E-14 YKL102C_d YKL102C_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
0.106 7.15E-10 YDR393W SHE9 hom Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex
0.105 1.29E-9 YNL145W MFA2 hom Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
0.104 1.35E-9 YBL035C POL12 het B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
0.102 2.69E-9 YBR085C-A YBR085C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
0.101 4.41E-9 YDR436W PPZ2 hom Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.100 6.44E-9 YBR087W RFC5 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.095 3.42E-8 YGR169C-A_p YGR169C-A_p hom Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication
0.095 3.85E-8 YOL084W PHM7 hom Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
0.095 4.16E-8 YLR285C-A_p YLR285C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.094 5.08E-8 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.092 9.37E-8 YLR448W RPL6B hom Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
0.092 1.00E-7 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication