YDR399W / HPT1

Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR399W / HPT1

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-4.36|P-value:1.72E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:4.57|P-value:6.15E-7 ID:SGTC_122|Compound:0236-0016|FD-Score:3.62|P-value:6.02E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.92|P-value:1.51E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:5.98|P-value:1.06E-10 ID:SGTC_735|Compound:3076-0345|FD-Score:-2.93|P-value:9.00E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:-4.80|P-value:1.64E-7 ID:SGTC_748|Compound:0136-0228|FD-Score:-3.40|P-value:1.47E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.54|P-value:8.31E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.96|P-value:6.62E-8 ID:SGTC_759|Compound:3486-0358|FD-Score:3.12|P-value:4.71E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.20|P-value:3.37E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.59|P-value:6.98E-5 ID:SGTC_950|Compound:1165-0508|FD-Score:4.09|P-value:6.87E-6 ID:SGTC_967|Compound:1306-0166|FD-Score:3.11|P-value:4.86E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.92|P-value:1.57E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:2.95|P-value:8.79E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.96|P-value:1.26E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.94|P-value:1.46E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.43|P-value:1.18E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.87|P-value:2.00E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.05|P-value:5.91E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:4.09|P-value:7.08E-6 ID:SGTC_1611|Compound:st001856|FD-Score:-3.11|P-value:4.68E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.37|P-value:1.76E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.10|P-value:5.06E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-2.94|P-value:8.57E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.61|P-value:6.31E-5 ID:SGTC_2149|Compound:5554786|FD-Score:3.26|P-value:2.67E-4 ID:SGTC_2305|Compound:7443522|FD-Score:4.78|P-value:1.93E-7 ID:SGTC_2314|Compound:7727897|FD-Score:4.06|P-value:8.00E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:4.81|P-value:1.61E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:5.51|P-value:2.41E-9 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.80|P-value:3.50E-10 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.09|P-value:4.92E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.26|P-value:3.04E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:6.45|P-value:3.52E-12 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.49|P-value:2.81E-9 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-5.10|P-value:2.82E-8 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.83|P-value:1.90E-13 ID:SGTC_2699|Compound:st077704|FD-Score:-3.04|P-value:5.94E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.78|P-value:2.80E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.09|P-value:5.04E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.23|P-value:2.96E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.99|P-value:5.84E-8 ID:SGTC_2792|Compound:7535235|FD-Score:3.75|P-value:3.42E-5 ID:SGTC_2804|Compound:7954240|FD-Score:4.56|P-value:6.47E-7 ID:SGTC_2815|Compound:7968870|FD-Score:-2.91|P-value:9.78E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.05|P-value:5.94E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.36|P-value:1.72E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:4.57|P-value:6.15E-7 ID:SGTC_122|Compound:0236-0016|FD-Score:3.62|P-value:6.02E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.92|P-value:1.51E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:5.98|P-value:1.06E-10 ID:SGTC_735|Compound:3076-0345|FD-Score:-2.93|P-value:9.00E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:-4.80|P-value:1.64E-7 ID:SGTC_748|Compound:0136-0228|FD-Score:-3.40|P-value:1.47E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.54|P-value:8.31E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.96|P-value:6.62E-8 ID:SGTC_759|Compound:3486-0358|FD-Score:3.12|P-value:4.71E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.20|P-value:3.37E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:3.59|P-value:6.98E-5 ID:SGTC_950|Compound:1165-0508|FD-Score:4.09|P-value:6.87E-6 ID:SGTC_967|Compound:1306-0166|FD-Score:3.11|P-value:4.86E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.92|P-value:1.57E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:2.95|P-value:8.79E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.96|P-value:1.26E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.94|P-value:1.46E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.43|P-value:1.18E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.87|P-value:2.00E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:3.05|P-value:5.91E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:4.09|P-value:7.08E-6 ID:SGTC_1611|Compound:st001856|FD-Score:-3.11|P-value:4.68E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.37|P-value:1.76E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.10|P-value:5.06E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-2.94|P-value:8.57E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.61|P-value:6.31E-5 ID:SGTC_2149|Compound:5554786|FD-Score:3.26|P-value:2.67E-4 ID:SGTC_2305|Compound:7443522|FD-Score:4.78|P-value:1.93E-7 ID:SGTC_2314|Compound:7727897|FD-Score:4.06|P-value:8.00E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:4.81|P-value:1.61E-7 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:5.51|P-value:2.41E-9 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.80|P-value:3.50E-10 ID:SGTC_2522|Compound:biochanin a|FD-Score:-3.09|P-value:4.92E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.26|P-value:3.04E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:6.45|P-value:3.52E-12 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.49|P-value:2.81E-9 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-5.10|P-value:2.82E-8 ID:SGTC_2638|Compound:epoxomicin|FD-Score:6.83|P-value:1.90E-13 ID:SGTC_2699|Compound:st077704|FD-Score:-3.04|P-value:5.94E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.78|P-value:2.80E-5 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.09|P-value:5.04E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.23|P-value:2.96E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.99|P-value:5.84E-8 ID:SGTC_2792|Compound:7535235|FD-Score:3.75|P-value:3.42E-5 ID:SGTC_2804|Compound:7954240|FD-Score:4.56|P-value:6.47E-7 ID:SGTC_2815|Compound:7968870|FD-Score:-2.91|P-value:9.78E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.05|P-value:5.94E-4

Top fitness defect scores for YDR399W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2638 epoxomicin 6.83 18.0 µM 1.90E-13 2 4.12E-12
2 SGTC_2630 antimycin a 6.45 100.0 µM 3.52E-12 mitochondrial processes 9 5.48E-11
3 SGTC_245 methotrexate 5.98 289.6 µM 1.06E-10 12 1.13E-9
4 SGTC_2514 salinomycin 5.80 42.7 µM 3.50E-10 16 3.25E-9
5 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 5.51 54.6 µM 2.41E-9 RPP1 & pyrimidine depletion 10 1.80E-8
6 SGTC_2633 5,7-dihydroxyisoflavone 5.49 100.0 µM 2.81E-9 9 2.06E-8
7 SGTC_2779 methotrexate 4.99 400.0 µM 5.84E-8 17 3.06E-7
8 SGTC_2508 celastrol 4.81 40.5 µM 1.61E-7 PDR1 13 7.52E-7
9 SGTC_2305 7443522 4.78 69.2 µM 1.93E-7 4 8.84E-7
10 SGTC_78 0336-0123 4.57 156.8 µM 6.15E-7 14 2.48E-6
11 SGTC_2804 7954240 4.56 19.5 µM 6.47E-7 22 2.59E-6
12 SGTC_2566 crystal violet carbinol 4.26 226.0 nM 3.04E-6 34 1.03E-5
13 SGTC_950 1165-0508 4.09 94.1 µM 6.87E-6 28 2.12E-5
14 SGTC_1359 1582-0056 4.09 520.0 nM 7.08E-6 18 2.18E-5
15 SGTC_2314 7727897 4.06 200.0 µM 8.00E-6 19 2.43E-5
16 SGTC_1239 0337-0584 3.94 35.4 µM 1.46E-5 unfolded protein response 54 4.15E-5
17 SGTC_1025 k007-0731 3.92 70.0 µM 1.57E-5 12 4.44E-5
18 SGTC_1250 0485-0349 3.87 40.2 µM 2.00E-5 39 5.51E-5
19 SGTC_2792 7535235 3.75 81.8 µM 3.42E-5 fatty acid desaturase (OLE1) 38 8.86E-5
20 SGTC_122 0236-0016 3.62 402.4 µM 6.02E-5 RNA processing & uracil transport 46 1.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.219 1.01E-37 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.167 1.79E-22 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.166 2.91E-22 YER056C FCY2 hom Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
0.162 4.49E-21 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.157 5.19E-20 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.145 2.93E-17 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.143 9.56E-17 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.140 4.27E-16 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.133 1.02E-14 YGL243W TAD1 hom tRNA-specific adenosine deaminase, deaminates adenosine-37 to inosine in tRNA-Ala
0.125 3.90E-13 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.124 4.55E-13 YLR267W_p BOP2_p hom Protein of unknown function
0.122 1.54E-12 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.119 4.55E-12 YDL008W APC11 het Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
0.116 1.75E-11 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.115 2.40E-11 YJR092W BUD4 hom Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p