YDR461W / MFA1

Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR461W / MFA1

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.27|P-value:5.70E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.23|P-value:1.28E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.57|P-value:1.88E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.24|P-value:6.27E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.64|P-value:1.32E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.18|P-value:7.73E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.11|P-value:9.06E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-3.36|P-value:3.80E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.76|P-value:9.03E-5 ID:SGTC_949|Compound:1165-0505|FD-Score:-3.96|P-value:3.64E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:3.49|P-value:2.60E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.82|P-value:6.97E-7 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.92|P-value:4.39E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:-3.76|P-value:8.45E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-4.24|P-value:1.08E-5 ID:SGTC_1089|Compound:terbinafine|FD-Score:5.05|P-value:2.52E-7 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.33|P-value:4.18E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.84|P-value:6.72E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.90|P-value:5.22E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.89|P-value:5.45E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.80|P-value:7.82E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.63|P-value:1.51E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.35|P-value:4.34E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.25|P-value:6.07E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:-3.33|P-value:4.18E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:-3.16|P-value:7.82E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-3.85|P-value:5.74E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-3.51|P-value:2.19E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.29|P-value:4.90E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.23|P-value:6.52E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.66|P-value:1.23E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.47|P-value:2.79E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.51|P-value:2.42E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-5.10|P-value:1.66E-7 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.68|P-value:1.26E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.64|P-value:1.44E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.32|P-value:4.40E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.17|P-value:7.39E-4 ID:SGTC_2942|Compound:9052777|FD-Score:-3.38|P-value:3.58E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.34|P-value:4.08E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.18|P-value:7.17E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:3.27|P-value:5.70E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.23|P-value:1.28E-5 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.57|P-value:1.88E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.24|P-value:6.27E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.64|P-value:1.32E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.18|P-value:7.73E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.11|P-value:9.06E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:-3.36|P-value:3.80E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.76|P-value:9.03E-5 ID:SGTC_949|Compound:1165-0505|FD-Score:-3.96|P-value:3.64E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:3.49|P-value:2.60E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.82|P-value:6.97E-7 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.92|P-value:4.39E-5 ID:SGTC_1067|Compound:clotrimazole|FD-Score:-3.76|P-value:8.45E-5 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-4.24|P-value:1.08E-5 ID:SGTC_1089|Compound:terbinafine|FD-Score:5.05|P-value:2.52E-7 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.33|P-value:4.18E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.84|P-value:6.72E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.90|P-value:5.22E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.89|P-value:5.45E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.80|P-value:7.82E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.63|P-value:1.51E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.35|P-value:4.34E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.25|P-value:6.07E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:-3.33|P-value:4.18E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:-3.16|P-value:7.82E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-3.85|P-value:5.74E-5 ID:SGTC_1870|Compound:st057514|FD-Score:-3.51|P-value:2.19E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.29|P-value:4.90E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.23|P-value:6.52E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.66|P-value:1.23E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.47|P-value:2.79E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.51|P-value:2.42E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-5.10|P-value:1.66E-7 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.68|P-value:1.26E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.64|P-value:1.44E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.32|P-value:4.40E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.17|P-value:7.39E-4 ID:SGTC_2942|Compound:9052777|FD-Score:-3.38|P-value:3.58E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.34|P-value:4.08E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.18|P-value:7.17E-4

Top fitness defect scores for YDR461W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1089 terbinafine 5.05 2.2 µM 2.52E-7 5 2.25E-7
2 SGTC_41 0478-0632 4.23 82.6 µM 1.28E-5 23 1.17E-5
3 SGTC_1199 1638-0010 3.90 4.5 µM 5.22E-5 endomembrane recycling 26 4.82E-5
4 SGTC_1259 0671-0132 3.89 43.0 µM 5.45E-5 25 5.03E-5
5 SGTC_1171 0368-0077 3.84 21.4 µM 6.72E-5 22 6.22E-5
6 SGTC_1339 1486-1321 3.80 22.7 µM 7.82E-5 ERG2 27 7.24E-5
7 SGTC_797 k781-0471 3.76 270.0 µM 9.03E-5 RPP1 & pyrimidine depletion 27 8.37E-5
8 SGTC_2519 cryptotanshinone 3.68 88.0 µM 1.26E-4 heme biosynthesis & mitochondrial translocase 19 1.17E-4
9 SGTC_2524 avocatin b 3.64 10.6 µM 1.44E-4 29 1.34E-4
10 SGTC_1375 2910-0797 3.63 186.0 µM 1.51E-4 35 1.40E-4
11 SGTC_265 mycophenolic acid 3.57 32.9 µM 1.88E-4 RPP1 & pyrimidine depletion 33 1.75E-4
12 SGTC_2346 9031566 3.51 143.8 µM 2.42E-4 Golgi 42 2.26E-4
13 SGTC_971 1315-0384 3.49 26.7 µM 2.60E-4 cell wall signaling 39 2.43E-4
14 SGTC_2308 7584454 3.47 197.6 µM 2.79E-4 38 2.61E-4
15 SGTC_1482 3346-2049 3.35 8.5 µM 4.34E-4 44 4.06E-4
16 SGTC_38 0929-0051 3.27 58.3 µM 5.70E-4 46 5.34E-4
17 SGTC_1505 k297-0037 3.25 20.6 µM 6.07E-4 41 5.69E-4
18 SGTC_334 1502-1024 3.24 68.5 µM 6.27E-4 30 5.88E-4
19 SGTC_2127 5253358 3.23 187.9 µM 6.52E-4 49 6.12E-4
20 SGTC_497 capsazepine 3.18 133.0 µM 7.73E-4 38 7.26E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.106 9.07E-10 YLL028W TPO1 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane
0.104 1.73E-9 YER152C YER152C hom Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
0.095 3.49E-8 YMR008C PLB1 hom Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol
0.087 4.47E-7 YML116W ATR1 hom Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.086 6.22E-7 YHR061C GIC1 hom Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress
0.086 6.52E-7 YDR035W ARO3 hom 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
0.083 1.76E-6 YOL034W SMC5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
0.082 1.79E-6 YLL023C POM33 hom Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
0.082 1.83E-6 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.078 5.54E-6 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.077 9.44E-6 YDR152W GIR2 hom Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein
0.076 9.73E-6 YOL043C NTG2 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
0.076 1.05E-5 YGL039W YGL039W hom Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols
0.076 1.07E-5 YPR106W ISR1 hom Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
0.073 2.58E-5 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV