YDR477W / SNF1

AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR477W / SNF1

Click on Significant Values for Screen Details ID:SGTC_278|Compound:4092-0821|FD-Score:-3.42|P-value:2.22E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-3.04|P-value:8.78E-4 ID:SGTC_443|Compound:clozapine|FD-Score:-4.00|P-value:1.93E-5 ID:SGTC_444|Compound:curcumin|FD-Score:-4.15|P-value:1.01E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-3.32|P-value:3.17E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.36|P-value:2.76E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-4.34|P-value:4.07E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.23|P-value:4.49E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.21|P-value:4.87E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:-7.53|P-value:5.33E-15 ID:SGTC_1186|Compound:1319-0216|FD-Score:-3.43|P-value:2.07E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:-5.58|P-value:5.02E-9 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.29|P-value:3.62E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:-3.04|P-value:8.90E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.52|P-value:1.47E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.14|P-value:1.05E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.08|P-value:7.59E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:-3.09|P-value:7.47E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.31|P-value:3.29E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-5.25|P-value:3.47E-8 ID:SGTC_2282|Compound:7944268|FD-Score:-4.00|P-value:1.98E-5 ID:SGTC_2339|Compound:9000433|FD-Score:-4.22|P-value:7.18E-6 ID:SGTC_2809|Compound:7964133|FD-Score:-3.31|P-value:3.34E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-4.00|P-value:1.99E-5 ID:SGTC_2812|Compound:7970281|FD-Score:-3.78|P-value:5.11E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.52|P-value:1.51E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.38|P-value:2.60E-4 ID:SGTC_2928|Compound:9011484|FD-Score:-3.79|P-value:4.84E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.09|P-value:7.52E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.32|P-value:3.26E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-3.69|P-value:7.37E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-5.16|P-value:5.66E-8 ID:SGTC_3236|Compound:9132701|FD-Score:-5.74|P-value:1.85E-9 ID:SGTC_3278|Compound:9139605|FD-Score:-3.49|P-value:1.68E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:-3.42|P-value:2.22E-4 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:-3.04|P-value:8.78E-4 ID:SGTC_443|Compound:clozapine|FD-Score:-4.00|P-value:1.93E-5 ID:SGTC_444|Compound:curcumin|FD-Score:-4.15|P-value:1.01E-5 ID:SGTC_451|Compound:chloral hydrate|FD-Score:-3.32|P-value:3.17E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.36|P-value:2.76E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:-4.34|P-value:4.07E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.23|P-value:4.49E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.21|P-value:4.87E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:-7.53|P-value:5.33E-15 ID:SGTC_1186|Compound:1319-0216|FD-Score:-3.43|P-value:2.07E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:-5.58|P-value:5.02E-9 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.29|P-value:3.62E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:-3.04|P-value:8.90E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.52|P-value:1.47E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.14|P-value:1.05E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.08|P-value:7.59E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:-3.09|P-value:7.47E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.31|P-value:3.29E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-5.25|P-value:3.47E-8 ID:SGTC_2282|Compound:7944268|FD-Score:-4.00|P-value:1.98E-5 ID:SGTC_2339|Compound:9000433|FD-Score:-4.22|P-value:7.18E-6 ID:SGTC_2809|Compound:7964133|FD-Score:-3.31|P-value:3.34E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-4.00|P-value:1.99E-5 ID:SGTC_2812|Compound:7970281|FD-Score:-3.78|P-value:5.11E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.52|P-value:1.51E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.38|P-value:2.60E-4 ID:SGTC_2928|Compound:9011484|FD-Score:-3.79|P-value:4.84E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.09|P-value:7.52E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.32|P-value:3.26E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-3.69|P-value:7.37E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-5.16|P-value:5.66E-8 ID:SGTC_3236|Compound:9132701|FD-Score:-5.74|P-value:1.85E-9 ID:SGTC_3278|Compound:9139605|FD-Score:-3.49|P-value:1.68E-4

Top fitness defect scores for YDR477W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_48 0382-0018 2.65 55.0 µM 0.00330 72 0.00399
2 SGTC_2286 7945697 2.52 125.0 µM 0.00492 163 0.00585
3 SGTC_3224 9130643 2.52 49.5 µM 0.00494 RPP1 & pyrimidine depletion 130 0.00586
4 SGTC_3217 9129673 2.44 49.5 µM 0.00625 155 0.00735
5 SGTC_2952 9073731 2.44 13.8 µM 0.00627 tubulin folding & SWR complex 177 0.00737
6 SGTC_3047 9092122 2.39 49.5 µM 0.00719 134 0.00840
7 SGTC_54 0159-0055 2.38 9.0 µM 0.00732 ERG2 136 0.00855
8 SGTC_2258 7960471 2.37 200.0 µM 0.00755 99 0.00880
9 SGTC_686 1319-0111 2.37 510.0 µM 0.00765 60S ribosome export 137 0.00891
10 SGTC_2431 5490079 2.32 200.0 µM 0.00889 170 0.01030
11 SGTC_3299 9121982 2.28 43.1 µM 0.00971 170 0.01120
12 SGTC_1856 st057054 2.28 55.7 µM 0.00974 137 0.01120
13 SGTC_3306 9127676 2.28 59.8 µM 0.00975 154 0.01120
14 SGTC_2636 apigenin dimethyl ether 2.28 100.0 µM 0.00990 201 0.01140
15 SGTC_2290 7904846 2.24 200.0 µM 0.01110 196 0.01270
16 SGTC_2055 5235184 2.22 200.0 µM 0.01160 ERG2 186 0.01320
17 SGTC_2289 7946404 2.22 165.1 µM 0.01160 261 0.01330
18 SGTC_2424 5537928 2.20 59.2 µM 0.01220 206 0.01390
19 SGTC_1909 st060837 2.17 51.5 µM 0.01330 212 0.01510
20 SGTC_2411 st077133 2.16 39.9 µM 0.01340 RNA pol III & RNase P/MRP 223 0.01520

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.195 3.43E-30 YBL099W ATP1 hom Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.101 4.77E-9 YDR382W RPP2B hom Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
0.099 1.03E-8 YKL206C ADD66 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.096 2.46E-8 YNL118C DCP2 het Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
0.081 2.85E-6 YDL054C MCH1 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.072 3.01E-5 YJL097W PHS1 het Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
0.072 3.18E-5 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.072 3.27E-5 YDR450W RPS18A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
0.071 3.54E-5 YGR264C MES1 het Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
0.070 5.06E-5 YIR033W MGA2 hom ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
0.068 8.37E-5 YPL098C MGR2 hom Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
0.067 1.14E-4 YFR031C-A RPL2A hom Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
0.062 3.23E-4 YBR173C UMP1 hom Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
0.062 3.60E-4 YJR024C MDE1 hom 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
0.061 3.91E-4 YGR184C UBR1 hom E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)